ENERGY & FUELS
·
2024
·
IF 5.2
Jun Yang,Guancheng Jiang,Shengming Huang,Jingtian Yi,Tengfei Dong,Yinbo He,Lili Yang,Qi Feng,Ge Wang
In this study, a nanobiocatalyst [α-amylase/γ-aminopropyltriethoxysilane-modified silica nanoparticles (A-SNPs)] was prepared for efficient mudcake removal and reservoir damage control during horizontal drilling and openhole completion. The molecular structure, thermal stability, and colloidal properties of the nanobiocatalyst were characterized by using FTIR, TGA, particle size, zeta potential, SEM, and TEM tests. The results confirmed that A-SNPs were securely encapsulated on the surface of α-amylase via covalent imine bonding (C═N), significantly enhancing the stability of α-amylase. Mudcake and polymer plugging removal experiments demonstrated that soaking mudcake in a 1% nanobiocatalyst solution for 4 h reduced the filtrate return time to under 10 min. Additionally, polymer removal efficiency exceeded 63.17% at 500 psi. Core flow experiments combined with NMR analysis revealed that the nanobiocatalyst effectively mitigated permeability damage at 95 °C, achieving a core permeability restoration value of 82.88%. The nanobiocatalyst significantly reduced the fluid flow resistance and improved hydrocarbon availability within the reservoir porous medium. In conclusion, nanobiocatalysts have great potential for application in oil and gas drilling and hydrocarbon recovery.
研究领域:
石油工程
·
纳米技术
·
生物催化
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2024
·
IF 9.7
Li Chen,Zhigang Yi,Yaoning Chen,Yuanping Li,Hongjuan Jiang,Jun Wang,Yanrong Chen,Yaoqin Nie,Mengwei Luo,Qianruyu Wang,Wei Zhang,Yanting Wu
The current research studied how Fe 3 O 4 nanomaterials (NMs) and CaO 2 affect humification and Cr(VI) immobilization and reduction during the composting of oil-tea Camellia meal and Cr-contaminated soil. The results showed that Fe 3 O 4 NMs and CaO 2 successfully construct a Fenton-like reaction in this system. The excitation-emission matrix-parallel factor (EEM-PARAFAC) demonstrated that this Fenton-like treatment increased the generation of humic acids and accelerated the humification. Meantime, RES-Cr increased by 5.91 % and Cr(VI) decreased by 16.36 % in the treatment group with CaO 2 and Fe 3 O 4 NMs after 60 days. Moreover, the microbial results showed that Fe 3 O 4 NMs and CaO 2 could promote the enrichment of Cr(VI) reducing bacteria, e.g., Bacillus , Pseudomonas , and Psychrobacter , and promote Cr(VI) reduction. This study gives a novel view and theoretical reference to remediate Cr(VI) pollution through composting.
研究领域:
环境科学
·
土壤学
·
微生物学
·
废弃物管理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL GEOLOGY
·
2025
·
IF 3.6
Chaorong Chen,Sailan Wang,Yixuan Yang,Gaofeng Wang,Jing Liu,Qingze Chen,Fei Ge,Runliang Zhu
Ferrihydrite is an important geosorbent and catalyst that is ubiquitous in natural environments, and plays a critical role in regulating the speciation and migration of hexavalent chromium (Cr(VI)). Phosphate has a strong affinity for ferrihydrite surfaces, but it remains unclear how phosphate influences the immobilization of Cr(VI) by ferrihydrite in sunlight-influenced surface environments. Herein, the surface interactions of ferrihydrite and Cr(VI) in the presence of phosphate were studied under mercury lamp irradiation. Results showed that mercury lamp irradiation significantly attenuated the inhibitory effect of phosphate on Cr(VI) removal by ferrihydrite compared to that under dark conditions. X-ray photoelectron spectroscopic (XPS) analysis revealed that Cr(III) was the predominant chromium species immobilized on ferrihydrite surfaces. Specifically, the reduction of Cr(VI) produced CrPO 4 precipitates, whereas the released Fe(III) can also co-adsorb with phosphate (i.e., FePO 4 ) onto ferrihydrite surfaces. The reduction of Cr(VI) mediated by the generated Fe(II) species and photoelectrons effectively reduces the electrostatic repulsion between Cr(VI) and phosphate, thereby enhancing the immobilization of these two ions on ferrihydrite. Energy dispersive X-ray spectrometer (EDS) results provide direct quantitative evidence that mercury lamp irradiation substantially enhances the immobilization of Cr(VI) and phosphate by ferrihydrite. The above-described results account for the effect of phosphate on the light-induced coupling process of ferrihydrite reductive dissolution and Cr(VI) reductive immobilization, advancing our understanding of the geochemical cycle of Cr(VI) pollution in sunlight-influenced surface environments.
研究领域:
环境化学
·
地球化学
·
污染修复
·
光催化
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2018
·
IF 3.141
Yan Li,Jiangtao Liu,Kehu Zhang,Lei Lei,Zhongli Lei
A hybrid polymer–MOFs architecture UiO-66-NH2@PMAA was synthesized by tethering polymethacrylic acid (PMAA) onto the surface of the metal–organic frameworks UiO-66-NH2, and it was further employed for pectinase immobilization by electrostatic interactions. The hybrid architecture was prepared through a combination of atom transfer radical polymerization (ATRP), click chemistry and postsynthetic modification (PSM). The optimal immobilization parameters were at 25 °C and pH 4.0 with 10 U/mL of pectinase for 2 h, under which pectinase showed the highest enzymatic activity (1.215 U/mg) and protein loading (448.5 mg/g), respectively. Compared with free pectinase, the immobilized was found to exhibit higher pH, thermo, and storage stability. Additionally, the immobilized pectinase had remarkable reusability, the residual activity reached as high as 81% after 8 cycles continuously. Meanwhile, the desorption of immobilized pectinase can be achieved by changing pH values of the medium. Obviously, the UiO-66-NH2@PMAA with attractive properties is highly promising for enzyme immobilization.
研究领域:
生物化学
·
材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
·
2023
·Vol. 333
·期 1
·545-556
·
IF 1.6
Tang Haoming,Zhang Mingzhu,Ran Fengya,Sun Shiyong,Golubev Yevgeny Aleksandrovich,Zhao Qian,Lin Sen,Duan Tao,Kotova Olga Borisovna
The Cu 2 O/Cu modified shungite (ShC) absorbent (Cu 2 O/Cu-ShC) was synthesized by hydrothermal method, benefiting from the mineral backbone and Cu 2 O/Cu particles, Cu 2 O/Cu-ShC can remove ~ 96% of I − within 60 min, the batch experiments showed that the I − adsorption process follows pseudo-second-order model and Langmuir model with maximum adsorption capacity of 10.99 mg/g. The high binding affinity between Cu + and I − contributes effective removal of I − and excellent selectivity at high concentrations of competing ions. This work highlights the feasibility of Cu 2 O/Cu-ShC for the radioactive iodine separation, and provides a promising sorbent material for large-scale purification of radioactive iodine anions in wastewater.
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
·
2012
·Vol. 87
·期 8
·1083-1088
·
IF 2.168
Liwei Sun,Hao Liang,Qipeng Yuan,Tianxin Wang,Hongchao Zhang
BACKGROUND: Most enzymes, including protease, play a key role in biotechnology, but their use is quite limited due to poor recovery, limited reusability and instability. Immobilized enzymes offer advantages over free enzymes. This paper reports a simple method for the preparation of immobilized papain, an endolytic cysteine protease (EC: 3.4.22.2), on carboxyl-activated silica nanoparticles. RESULTS: The carboxyl-activated carriers produced reactive carboxyl groups which then react with the free amino groups of enzyme to give peptide bonds ( CO NH ). The results showed that the thermal and pH stabilities of the immobilized papain were higher than those of free enzyme. And the apparent K m value of the immobilized papain was 1.26 times higher than that of free enzyme. Moreover, the immobilized papain retained more than 45% of the original activity after ten reuses continuously. CONCLUSION: The results indicated that papain was successfully immobilized on the surface of the activated carriers. The immobilized papain had not only higher activity recovery, but also better stability, reusability and environmental adaptability. Copyright © 2012 Society of Chemical Industry
研究领域:
酶工程
·
纳米生物技术
·
生物催化
·
应用微生物学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Sizhu Ren,Ziyuan Wang,Muhammad Bilal,Yuxiao Feng,Yunhong Jiang,Shiru Jia,Jiandong Cui
Multienzymatic conversion of carbon dioxide (CO 2 ) into chemicals has been extensively studied. However, regeneration and reuse of co-factor are still the main problems for the efficient conversion of CO 2 . In this study, a nanoscale multienzyme reactor was constructed by encapsulating simultaneously carbonic anhydrase (CA), formate dehydrogenase (FateDH), co-factor (NADH), and glutamate dehydrogenases (GDH) into ZIF-8. In the multienzyme reactors, cationic polyelectrolyte (polyethyleneimine, PEI) was doped in the ZIF-8 by dissolving it in the precursors of ZIF-8. Co-factor (NADH) was anchored in ZIF-8 by ion exchange between PEI (positive charge) and co-factor (negative charge), and regenerated through GDH embedded in the ZIF-8, thus keeping high activity of FateDH. Activity recovery of FateDH in the multienzyme reactors reached 50%. Furthermore, the dissolution of CO 2 in the reaction solution was increased significantly by the combination of CA and ZIF-8. As a result, the nanoscale multienzyme reactor exhibited superior capacity for conversion of CO 2 to formate. Compared with free multienzyme system, formate yield was increased 4.6-fold by using the nanoscale multienzyme reactor. Furthermore, the nanoscale multienzyme reactor still retained 50% of its original productivity after 8 cycles, indicating excellent reusability.
研究领域:
生物技术
·
酶工程
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DALTON TRANSACTIONS
·
2017
·Vol. 46
·期 7
·2114-2121
·
IF 4.029
Gen Liu,Yan Xu,Yide Han,Junbiao Wu,Junli Xu,Hao Meng,Xia Zhang
A hierarchical zeolitic imidazolate framework-8 (micro/meso-ZIF-8) was fabricated by using cetyltrimethylammonium bromide as a structure-controlling agent and L-histidine as co-templates. Compared to the conventional microporous ZIF-8 (micro-ZIF-8), the hierarchical porous structure of micro/meso-ZIF-8 contains micropores and maximum mesopores of around 35.6 nm. The as-prepared hierarchical micro/meso-ZIF-8 featured a large surface area and superior spontaneous adsorption activity than micro-ZIF-8 towards lysozyme (LZM), bovine hemoglobin (BHb) and bovine serum albumin (BSA), and the adsorption capacity increased with the decreasing of the protein size due to the molecule cutoff effects. The maximum adsorption capacity of LZM on micro/meso-ZIF-8 was higher than most of the reported results under similar adsorption conditions. The analyses of adsorption kinetics and thermodynamics implied that the adsorption mechanism mainly involved physical adsorption. Moreover, the micro/meso-ZIF-8 showed good thermal stability against temperature and excellent regeneration ability in the recycling adsorption experiments. This work proposed herein opens a broad application prospect of hierarchical MOFs in biological molecule separation, immobilization and enrichment.
研究领域:
生物化学
·
材料科学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2021
·
IF 3.171
Junyang Xu,Yanjun Jiang,Liya Zhou,Li Ma,Zhihong Huang,Jiafu Shi,Jing Gao,Ying He
Immobilization is an effective method to promote the application of enzyme industry for improving the stability and realizing recovery of enzyme. To some extent, the performance of immobilized enzyme depends on the choice of carrier material. Therefore, the development of new carrier materials has been one of the key issues concerned by enzyme immobilization researchers. In this work, a novel organic–inorganic hybrid material, nickel-carnosine complex (NiCar), was synthesized for the first time by solvothermal method. The obtained NiCar exhibits spherical morphology, hierarchical porosity and abundant unsaturated coordination nickel ions , which provide excellent anchoring sites for the immobilization of proteins. His-tagged organophosphate-degrading enzyme (OpdA) and ω-transaminase (ω-TA) were used as model enzymes to evaluate the performance of NiCar as a carrier. By a simple adsorption process , the enzyme molecules can be fixed on the particles of NiCar, and the stability and reusability are significantly improved. The analysis of protein adsorption on NiCar verified that the affinity adsorption between the imidazole functional group on the protein and the unsaturated coordination nickel ions on NiCar was the main force in the immobilization process, which provided an idea way for the development of new enzyme immobilization carriers.
研究领域:
生物化学
·
材料科学
·
酶工程
✓ 已验证
产品关联
DOI
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2018
·Vol. 41
·期 7
·1051-1060
·
IF 2.139
Han Juan,Rong Junhui,Wang Yun,Liu Qian,Tang Xu,Li Cheng,Ni Liang
Abstract Magnetic double-shell hybrid microspheres (Fe 3 O 4 @SiO 2 @p(NIPAM- co -GMA)) have been developed as a promising supported substrate for the immobilization of cellulase. Since the surface of the magnetic microspheres not only contains an epoxy group from GMA (glycidyl methacrylate) that can covalently bind to the enzyme, but also has an intelligent temperature response property from NIPAM ( N -isopropylacrylamide), the cellulase can be covalently bonded to the magnetic microspheres and have a temperature-sensitive capability. The immobilized cellulase has the recovery ability of cellulase activity after a high-temperature inactivation. The average amount and activity of immobilized enzymes, respectively, was 233 mg g −1 , 57.4 U mg −1 under the optimized conditions. The experimental results show that the immobilized cellulase has a wider catalytic temperature range, better temperature and storage stability. The residual activity still remained about 65.6% of the initial activity after the sixth catalysis run, which indicated that the immobilized enzyme had high reproducibility. Graphical abstract
研究领域:
生物化学
·
生物技术
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AICHE JOURNAL
·
2020
·Vol. 66
·期 9
·e16292
·
IF 3.519
Xin Yuan,Yu Liu,Fan Cao,Panliang Zhang,Jian Ou,Kewen Tang
Enzyme immobilization enhances the catalytic activity and stability of the enzyme, and also improves reusability. Metal–organic frameworks (MOFs), which possess diversified structures and porosity, have been used as excellent carriers for enzyme immobilization. Pseudomonas fluorescens lipase (PFL) has been successfully immobilized onto MOFs by covalent cross-linking to obtain a series of immobilized lipase (PFL@MOFs). PFL@MOFs are used for catalytic enantioselective hydrolysis of 2-(4-hydroxyphenyl) propionic acid ethyl ester enantiomers (2-HPPAEE) in aqueous medium and transesterification of 4-methoxymandelic acid enantiomers (4-MMA) in organic medium. The experimental results indicated that PFL@Uio-66(Zr) exhibits excellent enzymatic catalysis performances and high enantioselectives. In addition, to improve catalytic activity and reusability, PFL is modified by the polyethylene glycol (PEG) to prepare PEG-modified lipase (PFL-PEG), then PFL-PEG is immobilized onto Uio-66(Zr) to prepare PFL-PEG@Uio-66(Zr), demonstrating better reusability and catalytic activity compared with PFL@Uio-66(Zr).
研究领域:
生物化学
·
酶工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2018
·
IF 3.235
Xuejiao Liu,Wanwan Chen,Meiling Lian,Xu Chen,Yanluo Lu,Wensheng Yang
Metal-organic frameworks (MOFs) as a type of potential matrix for enzyme immobilization have attracted much interest. However, the sensitivity of most MOF-enzymatic electrochemical biosensors has not been satisfactory so far due to the poor conductivity and low apparent affinity of MOFs. In this work, new strategies have been presented: (i) using MOFs that themselves possess catalytic properties for substrates, so the synergistic catalysis by combining the MOFs and immobilized enzymes can be reinforced; (ii) introducing hydrophilic carbon nanomaterials and preparing MOF/carbon composites, not only the conductivity of the matrix but also the apparent affinity of immobilized enzyme for hydrophilic substrate can be remarkably enhanced. Horseradish peroxidase (HRP) and zeolite imidazolate framework-67(Co)/multi-walled carbon nanotube composite (ZIF-67(Co)/MWCNT) were adopted as the model enzyme and the immobilized matrix to illustrate and examine the proposed strategies. Compared with single component (ZIF-67(Co) or MWCNTs) based HRP electrochemical biosensors, the composite electrochemical biosensor displayed increased apparent substrate affinity (Michaelis constant K m reduced by ~75%) and 1.3-fold increased sensitivity in the detection of H 2 O 2 . The sensitivity (315 μA mM −1 cm −2 ) was also much larger than that of most previously reported MOF-HRP electrochemical biosensors. The proposed strategies are envisaged to be useful for other MOF-enzymes systems, which will be beneficial to the development of biocatalysis and biosensing.
研究领域:
生物化学
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）