ANALYTICAL CHEMISTRY
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2024
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IF 6.7
Xiaoyu Zhou,Yanqi Zou,Hongjuan Ru,Fei Yan,Jiyang Liu
Herein, we, for the first time, synthesize silver nanoparticles (Ag NPs) within the nanochannels of amino group-functionalized vertically ordered mesoporous silica films (NH2–VMSF) and investigate their coreaction accelerator role in the luminol-dissolved oxygen (O2) electrochemical stripping chemiluminescence (ESCL) system. The synthesized Ag NPs are capable of electrocatalytic reduction of O2 to superoxide radicals, and meanwhile, sliver ions (Ag+) electrochemically stripped from Ag NPs can promote the amount of luminol anion radicals, generating the boosted ECL intensity of the luminol-dissolved O2 system. This proposed Ag NPs@NH2–VMSF on the indium tin oxide electrode was applied to construct the ESCL aptasensor for quantitative determination of prostate-specific antigen (PSA), yielding a low detection limit [0.19 pg/mL (S/N = 3)] and a broad linear dynamic range (1 pg/mL to 100 ng/mL). Furthermore, good analytical performance of PSA in serum with satisfactory recoveries and low relative standard deviation values is achieved by our developed ESCL aptasensor, rendering it a convenient and sensitive method for PSA determination in clinical applications and further broadening the strategy of ESCL techniques.
研究领域:
纳米技术
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分析化学
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生物传感器
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电化学
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2021
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IF 10.618
Yan Sun,Peipei Li,Yu Zhu,Xiaohua Zhu,Youyu Zhang,Meiling Liu,Yang Liu
A sensitive electrogenerated chemiluminescence (ECL) biosensor for glucose was developed based on in situ growth of TiO 2 nanowires on Ti 3 C 2 MXenes (TiO 2 –Ti 3 C 2 ) as the nanoplatform. Via tuning the alkaline oxidation time, different amount of TiO 2 nanowires can be found on MXenes. An ECL biosensor for glucose was constructed by covalent immobilization of glucose oxidase (GODx) on the glycine functional TiO 2 –Ti 3 C 2 surface, with the ECL signal depending on the in-situ formation of H 2 O 2 via the specifically catalysis of glucose by GODx, resulting in the apparent increase of ECL signal. The TiO 2 –Ti 3 C 2 can also act as the catalyst for the oxidation of H 2 O 2 into O 2 to enhance the ECL of luminol. Based on this strategy, a highly sensitive ECL biosensor for glucose was obtained in wide concentration range of 20 nM-12 mM with a low detection limit of 1.2 nM (S/N = 3). The synergistic effects of large surface area, excellent conductivity, and high catalytic activity of the TiO 2 –Ti 3 C 2 make the sensor highly sensitive toward glucose; the specific enzyme catalysis reaction promises excellent selectivity of the ECL sensor. The proposed biosensor has been employed to detect glucose in human serum, fruits, and sweat samples with excellent performance, providing a universal approach for glucose in various samples, which shows great prospect in clinical diagnostics and wearable sensors .
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高影响因子
ANALYTICAL CHEMISTRY
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2019
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IF 6.35
Shanshan Wang,Yangyang Zhao,Minmin Wang,Haijuan Li,Muhammad Saqib,Chunhua Ge,Xiangdong Zhang,Yongdong Jin
Electrochemiluminescence (ECL) has emerged as one of the most important methods for in vitro diagnosis and detection, but it is still limited in sensitivity for ultrasensitive biodetections. Fast and ultrasensitive detection of biomolecules is critical, especially for the clinical detection of cardiac troponin I (cTnI) for cardiac infarction diagnosis. In this study, an effective tactic was developed to enhance ECL efficiency of the luminol system, by combined use of Co2+-based metal organic frameworks (MOF), zeolitic imidazolate frameworks (ZIF-67), and luminol-capped Ag nanoparticles (luminol-AgNPs). The integration leads to a pronounced ∼115-fold enhancement in luminol ECL. On the basis of this fascinating sensing platform, a robust label-free ECL immunosensor was constructed for ultrasensitive detection of cTnI, the main marker of myocardial infarction, with good stability and a detection limit as low as 0.58 fg mL–1 (S/N = 3).
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高影响因子
TALANTA
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2022
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IF 6.556
Sijie Yuan,Ru Yu,Ying Tu,Yanhua Du,Xuan Feng,Fei Nie
Here, sulfonated polyaniline (SPAN) was decorated on the surface of copper-based metal organic frame (HKUST-1) and the composite was functionalized by luminol to construct a chemiluminescence (CL) hybrids (SPAN/HKUST-1@Luminol). The as-prepared SPAN/HKUST-1@Luminol demonstrated a great dispersion and stability performance in aqueous solution. Moreover, the resultant SPAN/HKUST-1@Luminol hybrids exhibited extremely strong CL properties, and the CL quantum yield was 136 times higher than that of luminol. In particular, it exhibited outstanding CL activity not only under alkaline conditions, but also under neutral conditions. The sensitive response of the hybrid to hydrogen peroxide was used to construct CL methods for the detection of hydrogen peroxide at a wide range of pH, with the detection limit of 60 nM at a neutral condition and 25 pM at alkaline condition. Due to strong and stable signal of the SPAN/HKUST-1@Luminol, the CL method provides a viable tool for determination of H 2 O 2 in biological systems and enabled the monitoring of stimulated production of H 2 O 2 released by living cells.
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高影响因子
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