NEW JOURNAL OF CHEMISTRY
·
2025
·
IF 2.5
Ruifang Wang,Jinbao Li,Xiaohua Tian,Zhuo Yan,Xiaohui Dai,Jiangdong Dai
This study presents a novel, eco-friendly method for enhancing the oil-fouling resistance of polyvinylidene fluoride (PVDF) membranes, employing ovalbumin (OVA) and tannic acid (TA) for surface modification. The modification process imparts superhydrophilic and underwater superoleophobic properties to the membranes, significantly improving their performance in oil-water emulsion separation. Traditional separation techniques often fall short in effectively handling complex and stable oil-in-water emulsions produced by various industrial sectors. Membrane technology, while promising, is frequently hampered by fouling issues that reduce permeance and increase operational costs. Our method leverages the unique molecular structure of TA, known for its hydrophilic hydroxyl and carboxyl groups and hydrophobic phenolic ester groups, to achieve robust membrane modifications through simple immersion techniques. The interaction between OVA and TA forms a micro-nano rough structure on the membrane surface, enhancing its superhydrophilic and underwater superoleophobic characteristics. The modified PVDF@OVA-TA membranes exhibit a sliding contact angle of 2.6°, indicating extremely low oil adhesion. These membranes achieve high permeance values for various oil-in-water emulsions of 4812.2 L m-2 h-1 bar-1 for hexane-in-water, 3720.1 L m-2 h-1 bar-1 for petroleum ether-water, 3127.4 L m-2 h-1 bar-1 for dichloromethane-water, and 5587.4 L m-2 h-1 bar-1 for soybean oil-in-water with separation efficiencies exceeding 99%. Moreover, the membranes demonstrate exceptional stability and reusability, maintaining high separation efficiency and permeance after multiple cycles. This study underscores the importance of developing sustainable membrane technologies that align with green chemistry principles, utilizing non-toxic, naturally derived materials. The findings highlight the potential of OVA and TA in advancing oil-water separation technology, offering a practical solution to industrial wastewater challenges and contributing to more sustainable environmental practices.
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Xiaomei Ye,Shiru Mo,Mingyue Shen,Qiang Yu,Yi Chen,Chengyuan Wang,Xianxiang Chen,Jianhua Xie
Allergic asthma accounts for more than 50 percent of adult asthma in life. Glucocorticoids and leukotriene receptor antagonists are commonly used to treat asthma, but these drugs not only have high side effects but also cannot cure the disease. In contrast, natural active ingredients are relatively safe to the organism and show great potential for improving the organism. Polysaccharides, as the main active ingredient in aqueous extract, may be equally beneficial for biological activity. The aim of this study is to investigate the alleviating effect and mechanism of action of Ficus pumila fruit polysaccharide (FPP) and aqueous extract of Ficus pumila fruit (FPW) on mice with allergic asthma. The results showed that FPP and FPW could regulate the secretion of the cytokines IL-4, IL-5, IL-13, and IFN-γ. FPP and FPW also reduced the inflammation, mucus secretion, and collagen deposition in mouse lung tissue, and down-regulated the expression of matrix metallopeptidase-9 (MMP-9), tissue inhibitor of metalloproteinases-1 (TIMP-1), and α-smooth muscle actin (α-SMA) proteins. In addition, FPP and FPW modulated the intestinal microbiota by augmenting the alpha diversity and adjusting the taxonomic composition at the phylum and family levels, thereby ensured the sustenance of regular physiological processes in the body. This study demonstrated that FPP and FPW may attenuate asthma by inducing beneficial microbiota in the gut-lung axis and provide an effective basis for the future clinical treatment of allergic asthma with Ficus pumila and the development and utilization of new anti-asthma drugs.
✓ 已验证
产品关联
DOI
高影响因子
BMC Pharmacology & Toxicology
·
2024
·Vol. 25
·期 1
·1-12
·
IF 2.8
Tian Chunyan,Liu Qi,Zhang Xiaoyu,Li Zhuying
Background Calycosin, a flavonoid compound extracted from Astragalus membranaceus , has shown anti-asthma benefits in house dust mite-induced asthma. Recent studies have suggested that innate-type cells, including group 2 innate lymphoid cells (ILC2s) and macrophages, serve as incentives for type 2 immunity and targets for drug development in asthma. This work focuses on the effects of calycosin on the dysregulated ILC2s and macrophages in allergic asthma. Methods In vivo, the asthmatic mouse model was established with ovalbumin (OVA) sensitization and challenge, and calycosin was intraperitoneally administered at doses of 20 and 40 mg/kg. In vivo, mouse primary ILC2s were stimulated with interleukin (IL)-33 and mouse RAW264.7 macrophages were stimulated with IL-4 and IL-13 to establish the cell models. Cells were treated with calycosin at doses of 5 and 10 µM. Results In vivo, we observed significantly reduced numbers of eosinophils, neutrophils, monocyte macrophages and lymphocytes in the bronchoalveolar lavage fluid (BALF) of OVA-exposed mice with 40 mg/kg calycosin. Histopathological assessment showed that calycosin inhibited the airway inflammation and remodeling caused by OVA. Calycosin markedly decreased the up-regulated IL-4, IL-5, IL-13, IL-33, and suppression tumorigenicity 2 (ST2) induced by OVA in BALF and/or lung tissues of asthmatic mice. Calycosin repressed the augment of arginase 1 (ARG1), IL-10, chitinase-like 3 (YM1) and mannose receptor C-type 1 (MRC1) levels in the lung tissues of asthmatic mice. In vivo, calycosin inhibited the IL-33-induced activation as well as the increase of IL-4, IL-5, IL-13 and ST2 in ILC2s. Calycosin also repressed the increase of ARG1, IL-10, YM1 and MRC1 induced by IL-4 and IL-13 in RAW264.7 macrophages. In addition, we found that these changes were more significant in 40 mg/kg calycosin treatment than 20 mg/kg calycosin. Conclusions Collectively, this study showed that calycosin might attenuate OVA-induced airway inflammation and remodeling in asthmatic mice via preventing ILC2 activation and macrophage M2 polarization. Our study might contribute to further study of asthmatic therapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food & Function
·
2023
·Vol. 14
·期 8
·3779-3792
·
IF 6.1
Ruiling Li,Na Wu,Hui Xue,Binghong Gao,Huilan Liu,Tianfeng Han,Xiaobo Hu,Yonggang Tu,Yan Zhao
This study was designed to investigate the influence and effect mechanism of the filler type on the physicochemical properties, microbial numbers, and digestibility of ovalbumin emulsion gels (OEGs) during storage. Sunflower oil was emulsified with ovalbumin (20 mg mL−1) and Tween 80 (20 mg mL−1) separately to prepare ovalbumin emulsion gels (OEGs) that contained active and inactive fillers, respectively. The formed OEGs were stored at 4 °C for 0, 5, 10, 15, and 20 days. The active filler enhanced the gel hardness, water holding capacity, fat holding capacity, and surface hydrophobicity and decreased the digestibility and free sulfhydryl content during storage compared to control (unfilled) ovalbumin gel, whereas the inactive filler had the opposite effects. Protein aggregation diminished, lipid particle aggregation increased, and the amide A band shifted to a higher wavenumber for all three types of gel during storage, suggesting that the compact network structure of the OEG became rough and disordered with storage. The OEG with the active filler did not inhibit microbial growth, and the OEG with the inactive filler did not significantly promote the development of bacteria. In addition, the active filler delayed the in vitro digestion of the protein in the OEG throughout storage. Emulsion gels containing active filler facilitated the retention of the gel properties during storage, whereas emulsion gels containing inactive filler exacerbated the loss of the gel properties during storage.
✓ 已验证
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Yiting Zhang,Bing Li,Qianying Ding,Xiaoli Liu,Jiaxuan Ma,Xuan Yao,Zhijun Xia,Yuanyuan Liu,Yongguo Jin
Compared with natural proteins, protein microgels better enhance the performance and stability of high internal phase Pickering emulsions (HIPPEs), with promising food industry applications. In this study, ovalbumin-resveratrol (OVA-RES) composite microgel particles were synthesized via a top-down approach to fabricate HIPPEs, which showed significant improvements in viscoelasticity, storage, centrifugal, and thermal stability. To clarify the stabilization mechanism, the morphology, structure, and physicochemical properties of composite microgels under different RES concentrations were characterized. The results revealed that the optimal performance was attained at a RES concentration of 0.12 mM: at this concentration, RES induced a conformational transition of OVA primarily through hydrophobic interactions. Specifically, the α-helix content decreased from 23.65 % to 12.60 %, the β-sheet content increased from 36.55 % to 49.20 %, and the free sulfhydryl group content rose from 19.12 μmol/g to 21.57 μmol/g. Furthermore, the surface activity of the composite microgels was significantly enhanced, as evidenced by increased hydrophobicity, reduced surface tension, and an elevation in contact angle from 53.6° to 72.85°. In the present study, the emulsifying properties of OVA microgel particles were successfully enhanced through the incorporation of RES, providing new insights into natural protein-polyphenol composite microgels as efficient emulsifiers. Meanwhile, this work develops a feasible food-grade emulsion stabilization and delivery platform for the incorporation of RES into emulsion-based food systems. Future research will further focus on the bioactive functions of OVA-RES composite microgels in food systems, as well as their specific applications and industrialization potential in the food sector.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2026
·Vol. 15
·期 10
·1740
·
IF 6
Xiufang Huang,Yifan Shi,Yaobing Chen,Jianquan Kan,Kai Luo
This study aims to improve the utilization ofIdesia polycarpacrude oil (IPCO) in the food industry by developing high-internal-phase emulsions (HIPEs) stabilized through ternary complexes (ovalbumin (OVA), xanthan gum (XG), and tannic acid (TA)). IPCO is highly prone to oxidation due to its polyunsaturated fatty acid (PUFA) content. Optimal formulations were obtained by varying the component concentrations and assessing the structure, stability, and fat-substitution potential. Under conditions of 0.6%w/vXG and 2.5%w/vOVA-TA, HIPEs exhibited a smaller particle size (3.31 μm), high centrifugal oil retention (99.29%), strong emulsifying activity (49.91 m2/g), and excellent stability (99.69%). Additionally, a formulation with 1.5%w/vOVA-TA and 0.8%w/vXG showed good wettability, particle size, and stability, possibly due to excessive self-aggregation of XG, which caused a decrease in emulsion stability and wettability. Structural analysis (FTIR, XRD, SEM, CLSM) revealed that the stability of the emulsions was mainly attributed to strong non-covalent interactions and a dense interfacial adsorption layer. In cookie applications, substituting 25%w/wbutter or 50%w/wshortening with HIPEs resulted in comparable texture to the control group. GC–MS analysis of relative fatty acid composition showed that partial replacement with IPCO-based HIPEs shifted the final biscuits toward a lower relative proportion of palmitic acid (C16:0) and a higher relative proportion of linoleic acid (C18:2n6c). Overall, OVA–TA–XG-stabilized HIPEs effectively delayed the oxidation of IPCO and enabled partial replacement of conventional solid fats in biscuits, thereby shifting the relative fatty acid composition of the final products toward a higher proportion of unsaturated fatty acids.
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Jing-jing Zhang,Zong-cai Tu,Hui Wang,Yue-ming Hu,Peng-cheng Du,Yi-peng Yang
In this work, the mechanism of the effect of lipid oxidation on the IgG/IgE binding ability of ovalbumin (OVA) was investigated via the peroxyl radicals produced by 2, 2′-azobis (2-amidinopropane) dihydrochloride to simulate lipid oxidation. Results showed that the structure of OVA unfolded partially with an increase in oxidation degree, leading to the exposure of the allergenic epitopes and increasing the IgG/IgE binding ability of OVA. Nine oxidation sites were found on the α-helix, and these sites may unwind the α-helix and expose the allergenic epitopes on the OVA surface, leading to antibody recognition and combination. Consequently, the IgG/IgE binding ability of OVA was increased. In conclusion, the allergenic capacity of OVA can be promoted by modifying peroxyl radical oxidation in processing egg products.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Xiefei Li,Yingmei Wu,Fei Liu,Wenshan Duan,Lei Chen,Lujie Cheng,Yujie Huang,Xin Li,Chao Ai,Qun Huang
Unfavorable environmental factors limit the utilization of egg yolk lecithin in functional foods. Therefore, it is necessary to enhance its stability and effectiveness to cater to the diverse food needs of individuals. This study aimed to explore the feasibility of protecting and delivering egg yolk lecithin through O/W emulsions, which were stabilized using ovalbumin-fucoidan (OVA-FUC) binary complexes as emulsifiers. This study investigated the protective effect of OVA-FUC emulsion on egg yolk lecithin and its impact on delivery by examining the storage stability, in vitro release yield, and stability during in vitro digestion of egg yolk lecithin in the emulsion. The results indicate that egg yolk lecithin, when encapsulated in the OVA-FUC emulsion, exhibits a relatively stable behavior during simulated in vitro digestion. Additionally, encapsulation enhanced both the delivery efficiency and drug release of egg yolk lecithin. Furthermore, the thermal and light stability of egg yolk lecithin embedded in OVA-FUC emulsion was improved, significantly improving its storage performance. The emulsion, prepared using the OVA-FUC binary complex, effectively safeguarded and enhanced the delivery efficiency of bioactive substances as an encapsulating material, thus exhibiting immense potential for application in the active protection and delivery of such substances.
✓ 已验证
产品关联
DOI
高影响因子
PROGRESS IN ORGANIC COATINGS
·
2024
·
IF 6.5
Zhikun Yang,Biqiong Chen,Haroon Elrasheid Tahir,Zhihua Li,Xiaowei Huang,Mingrui Li,Ke Zhang,Bingxin Li,Xiaodong Zhai,Jiyong Shi,Xiaobo Zou,Jianbo Xiao
This work aims to develop a biodegradable film with enhanced mechanical, water-barrier, and antimicrobial characteristics for the preservation of cherry tomatoes . Here, a persimmon pectin/ovalbumin (PN/OVA) stabilized-neem essential oil Pickering emulsion (NOPE) was added into the gelatin/sodium alginate-based blend matrix (GS). Even the biodegradability and transmittance of NOPE-rich film were decreased as compared to the control film, its flexibility, oxygen-barrier, and water-barrier performances significantly ( P < 0.05) increased by 45.23 %, 32.32 %, and 37.02 %, respectively. The NOPE-loaded films destroyed the hyphal structure and reduced spore germination of Botrytis cinerea through slow-releasing neem essential oil (NO), in turn inhibiting the growth of B. cinerea . Finally, the prepared films were applied for cherry tomato preservation. Compared with the control, 2.0 wt%-loaded GS films greatly reduced the weight loss and disease incidence of cherry tomatoes by 57.33 and 72.4 %, respectively, owing to their excellent water resistance and slow-released properties. Thus, the addition of 2.0 wt% NOPE into GS film is of particular interest in the design of active biodegradable packaging films for fruit preservation.
✓ 已验证
产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Hedi Wen,Deju Zhang,Zhenzhen Ning,Zihao Li,Yan Zhang,Jingbo Liu,Ting Yu,Ting Zhang
To broaden the application of ovalbumin (OVA) in food formulations, it is meaningful to improve its foaming characteristics. This study aimed to investigate the effect of benzoic acid-based (3,4-dihydroxybenzoic acid, DA) and cinnamic acid-based polyphenols (trans-2-hydroxycinnamic acid, T2A) on the foaming properties of OVA at acidic (pH 3.0), neutral (pH 7.4) and alkaline (pH 9.0) pH conditions. Both the addition of polyphenols and acid treatment enhanced the foaming properties of OVA. Surface hydrophobicity, circular dichroism, free sulfhydryl groups, and Fourier transform infrared spectroscopy results indicated that after acidic workup, the presence of stronger hydrophobic interactions in OVA-polyphenol aggregates induced more disordered protein conformation and conversion or breakage of disulfide bonds. Particle size and zeta potential indicated that acidic treatment neutralized protein surface charges, further inducing self-aggregation and swelling of OVA, ultimately enhancing foaming properties. Comparatively, T2A exhibited better foam-inducing capacity due to its stronger interaction with OVA, leading to the unfolding of the OVA structure and the exposure of more hydrophobic groups. The intrinsic and 3-D fluorescence spectra experiments also confirmed that OVA-T2A aggregates at pH 3.0 had greater altered non-covalent interaction forces and protein secondary and tertiary structures compared to other complexes. This study provides a theoretical basis for designing protein formulations with excellent foaming properties.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 7
·1-10
·
IF 6.232
Xia Hongjun,Wang Lin,Li Chunyang,Tian Boshi,Li Qingfeng,Zhao Hui,Bai Quan
Fully porous silica microspheres (FPSM) with high specific surface area and hierarchical pore as matrix for HPLC were prepared. First, the porous silica nanospheres with controllable particle size and pore diameter were successfully synthesized using a dual-templating approach, the pore size of nanospheres can be increased to 18.4 nm by changing the molar ratios of octyltrimethylammonium bromide (TOMAB) and cetyltrimethyl ammonium bromide (CTAB), which is suitable for separation and analysis of biomolecules without pore enlargement. Then, the micron FPSM with hierarchical pore were synthesized by polymerization-induced colloid aggregation (PICA) using the porous nanospheres as a silicon source, which has a large mesoporous structure (35.2 nm) and high specific surface area (560 m 2 g −1 ). Subsequently, the FPSM modified with octadecyltrichlorosilane were studied as stationary phase for separation of cytochrome C, lysozyme, ribonuclease A, and ovalbumin, bovine serum albumin, and the baseline separation of five proteins was achieved within 1 min. The prepared column was also applied to the fast separation of digests of ovalbumin, and more chromatographic peaks were obtained compared to a commercial column under the same gradient elution conditions. In addition, the static-binding capacity of the functionalized FPSM for bovine serum albumin (BSA) was measured to be 276 mg g −1 , which was nearly twice the static adsorption given in literature. Therefore, these FPSM with high specific surface area and hierarchical pore structure are expected to have great potential for the separation of complex biological samples using HPLC.
A synthetic strategy was provided towards FPSM with hierarchical pores and high specific surface area using porous nanospheres as silicon source. The outstanding performance of the FPSM is that it has a high specific surface area while maintaining a large mesoporous size, which overcomes the disadvantage of sacrificing the specific surface area when increasing the pore size of porous silica microspheres prepared by using the traditional PICA method.
✓ 已验证
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2020
·
IF 4.972
Jian Gao,Chengzhi Liu,Jieyu Shi,Fangfang Ni,Qing Shen,Hujun Xie,Kuiwu Wang,Qunfang Lei,Wenjun Fang,Gerui Ren
The ovalbumin (OVA)-pectin (PEC)-sodium alginate (SA)-Vitamin D 3 (VD 3 ) complex nanoparticles were fabricated by antisolvent precipitation method, and the excellent encapsulation efficiency and loading capacity of VD 3 were obtained by 96.6% and 2.8%, respectively. Compared with ternary OVA-PEC-VD 3 complexes, the addition of SA with strong negative charge effectively regulated the OVA-PEC complexes and significantly improved the stability of OVA-PEC-SA-VD 3 complex nanoparticles with preferable size as small as 126 nm. The storage stability was also investigated after low temperature storage for 31 d, and the particle size of quaternary complexes was increased only 40 nm. In vitro digestion results elucidated that the complex nanoparticles had good stability in the simulated gastric fluid, and almost completely released in the simulated intestinal fluid confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) experiments and scanning electron microscope (SEM) images. The release kinetics study clarified that it was close to Fick release. Fluorescence and Fourier transform infrared spectroscopy (FTIR) experiments showed that quaternary complex nanoparticles were mainly combined by electrostatic, hydrophobic and hydrogen bonding interactions. The novel quaternary protein-polysaccharide complexes have excellent stability and great sustained-release performance for VD 3 , which may be helpful for the digestion and absorption of vitamin by human body, thus have potential applications in the food and drug industry.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）