MOLECULES
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2019
·Vol. 24
·期 24
·4487
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IF 3.06
Wenhui Li,Lidan Ran,Fei Liu,Ran Hou,Wei Zhao,Yingbiao Li,Chunyan Wang,Juan Dong
Grape seed extract (GSE) displays strong antioxidant activity, but its instability creates barriers to its applications. Herein, three HP-β-CD/GSE inclusion complexes with host–guest ratios of 1:0.5, 1:1, and 1:2 were successfully prepared by co-precipitation method to improve stability. Successful embedding of GSE in the HP-β-CD cavity was confirmed by fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), differential scanning calorimetry (DSC), and scanning electron microscopy (SEM) analyses. The Autodock Tools 1.5.6 was used to simulate the three-dimensional supramolecular structure of the inclusion complex of 2-hydroxypropyl-β-cyclodextrin and grape seed extract (HP-β-CD/GSE) by molecular docking. The MALDI-TOF-MS technology and chemical database Pubchem, and structural database PDB were combined to reconstitute the three-dimensional structure of target protein. The binding mode of the HP-β-CD/GSE inclusion complex to target protein was studied at the molecular level, and the antioxidant ability of the resulting HP-β-CD/GSE inclusion complexes was investigated by measuring 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging. The effects of HP-β-CD/GSE on myofibrillar protein from lamb tripe were also investigated under oxidative conditions. The positions and interactions of the binding sites of HP-β-CD/GSE inclusion complexes and target protein receptors were simulated by molecular docking. The results showed that HP-β-CD/GSE inclusion complexes were successfully prepared, optimally at a molar ratio of 1:2. At low (5 μmol/g) to medium (105 μmol/g) concentrations, HP-β-CD/GSE inclusion complexes decreased the carbonyl content, hydrophobicity, and protein aggregation of myofibrillar protein from lamb tripe, and increased the sulphydryl content. Furthermore, high concentration (155 μmol/g) of HP-β-CD/GSE inclusion complexes promoted protein oxidation.Keywords:grape seed extract;inclusion complex;hydroxypropyl cyclodextrin;antioxidant;myofibrillar protein;oxidation
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JOURNAL OF ETHNOPHARMACOLOGY
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2019
·
IF 3.414
Lei Peng,Chenxi Hu,Chaozheng Zhang,Yingying Lu,Shuli Man,Long Ma
Ethnopharmacological relevance Conyza blinii H.Lév. is a type of natural plant distributed in southwest of China. Its dried overground section can be used in traditional Chinese medicine (TCM) for treating infections, inflammations and occasionally cancers. CBS ( Conyza blinii saponin), mainly composed of triterpenoidal saponins of Conyza blinii H.Lév. CBS is considered as the major active fraction of this species. The current investigation have focused on the mechanisms of CBS with regard to its anti-cancer activity. Hence it is of high relevance of identifying the anti-cancer efficacy of ethnomedicine . Aim of the study To understand the anti-cancer mechanism of CBS using both in vitro and in vivo experiments. Materials and methods CBS ( Conyza blinii saponin) was obtained as described previously. We tested the anti-cancer activity of CBS using in vitro HeLa cell models and in vivo animal models. We adopted immunoblot , RT-PCR (reverse transcription polymerase chain reaction), luciferase reporter assay and flow cytometry to study relevant proteins, genes, pathways and cellular ROS (reactive oxygen species) responsible for anti-cancer activity of CBS. More, 24 tumour-xenografted mice were grouped randomly as ‘control’, ‘cisplatin’ (as positive control), ‘low dose’ and ‘high dose’ groups. The IL-1β, TNF-α, PGE2 and IL-2 in the blood serum and the tumour tissue of mice were measured. Results and conclusions We have found that CBS is capable of inducing apoptotic cancer cell death via both caspase-dependent and -independent pathways. CBS inhibits the activation of TGF-β signaling pathway in a dose- and time-dependent manner. Phospho-ERK, phospho-JNK and phospho-p38 MAPK are significantly suppressed by CBS. Furthermore, some inflammation mediators including IL-1β, TNF-α and PGE2 from animal samples were found decreased in CBS-treated mice models. In contrast, the level of IL-2, a cytokine commonly used for treating cancers, increased reversely. Last, we have discovered that CBS is able to decrease the expression of Nrf2, inhibit the activation of ARE and increase ROS level in HeLa cells. In summary, we have confirmed that the anti-cancer activity of CBS is possibly related to its TGF-β, MAPK, Nrf2 signaling pathways as well as some cancer related inflammation mediators and cytokines.
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Catalysts
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2019
·Vol. 9
·期 10
·813
·
IF 3.444
Tao Wen,Doudou Yan,Jie Meng,Jian Liu,Haiyan Xu
With co-reduction method, a new nanocomposite consisting of Cu and Pt (CuPt) was prepared in household. The morphology of CuPt alloy was characterized by scanning electron microscope (SEM) and transmission electron microscope (TEM). The diameter of CuPt was ca. 125 nm measured by dynamic light scattering (DLS). The atom ratio of Cu to Pt was determined to be about 1.2 by energy-dispersive X-ray (EDX). Enzyme-like activities of CuPt, including peroxidase (POD)-like and ascorbic acid oxidase (AAO)-like activities were examined with UV-Vis-NIR spectrometer. The CuPt was found to interact with α, α-diphenyl-β-picrylhydrazyl (DPPH) in the presence or absence of AA. With irradiation by light emitting diode (LED) light, the photocatalysis effect of CuPt on DPPH was investigated. With the addition of histidine, it was proved that singlet oxygen had an important role in the interaction involving CuPt. The new nanocomposite and the properties suggest various potentials of application.Keywords:CuPt;peroxidase (POD)-like;ascorbic acid oxidase (AAO)-like;DPPH
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DESALINATION
·
2022
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IF 11.211
Ying Wang,Yue Yang,Jixiao Wang,Zhi Wang,Song Zhao
Nanofiltration (NF) is regarded as a great potential technique for efficient treatment of industrial dyes and pharmaceutical effluents, however, its application still faces the challenges due to the trade-off among permeance , selectivity and anti-fouling property. Herein, a novel supramolecular enzymatic membrane was designed and successfully fabricated based on β-cyclodextrin (CD) and laccase (Lac), with the former providing supramolecular transport channels and the latter offering the pollutant degradation property . Specifically, after the host-guest complexation of CD with polyethylene glycol (PEG), the generated [email protected] supramolecules were introduced into interfacial polymerization (IP) via reacting with terephthaloyl chloride (TPC) to fabricate supramolecular membrane with “bead-like” channels. Furthermore, the laccase was immobilized onto the modified polysulfone (mPSf) surface to generate [email protected] @Lac-TPC/mPSf supramolecular enzymatic membrane for high-efficiency catalytic degradation of membrane foulants. The [email protected] /mPSf supramolecular membrane displayed excellent performance and operation stability for removal and desalination of small molecules, with pure water permeance up to 26.8 L·m −2 ·h −1 ·bar −1 , and satisfactory NaCl/dye and NaCl/medicine separation factors. Moreover, [email protected] @Lac-TPC/mPSf supramolecular enzymatic membrane showed an effective anti-fouling property with only 8.0 % permeance decline rate after 24 h continuous separation of tetracycline hydrochloride solution, suggesting its potential application for removal and desalination of small molecules.
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EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
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2015
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IF 3.447
Xu-Meng Zhang,Hui Guo,Zai-Shun Li,Fu-Hang Song,Wei-Min Wang,Huan-Qin Dai,Li-Xin Zhang,Jian-Guo Wang
Methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE) have caused an increasing mortality rate, which means that antibiotic resistance is becoming an important health issue. In the course to screen new agents for resistant bacteria, we identified that a series of isatin-β-thiosemicarbazones (IBTs) could inhibit the growth of MRSA and VRE. This was the first time that the “familiar” IBT compounds exhibited significant anti Gram-positive pathogen activity. Against a clinical isolated MRSA strain, 20 of the 51 synthesized compounds showed minimum inhibitory concentration (MIC) data of 0.78 mg/L and another 12 novel compounds had MICs of 0.39 mg/L. Moreover, these compounds also inhibited Enterococcus faecalis and VRE at similar levels, indicating that IBTs might have different mode of action compared with vancomycin . For these IBTs, comparative field analysis (CoMFA) models were further established to understand the structure–activity relationships in order to design new compounds from steric and electrostatic contributions. This work has suggested that IBTs can be considered as potential lead compounds to discover antibacterial inhibitors to combat drug resistance.
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CHEMICAL ENGINEERING JOURNAL
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2023
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IF 15.1
Chao Wang,Baokang Dang,Hanwei Wang,Yipeng Chen,Yushan Yang,Yingying Li,Ye Xiong
The assembly of heterogeneous photocatalysts with unique structures by 2D heterojunctions is an advanced strategy for efficient solar energy conversion. However, heterogeneous photocatalysts constructed from 2D materials will suffer from high photogenerated charge recombination and low light absorption due to the arbitrary stacking and weak bonding of lamellae. Here, flower-like Bi 4 O 5 I 2 / β -Bi 2 O 3 (FL-Bi 4 O 5 I 2 / β -Bi 2 O 3 ) heterojunctions are developed in situ using FL-δ-Bi 2 O 3 as the starting material. It is noteworthy that the basic compositional form of the material was well maintained throughout the synthesis. Besides, due to the random reaction of iodide ions with the exposed surface of δ-Bi 2 O 3 , multiple heterogeneous regions are displayed on the basic lamellar units constituting the Bi 4 O 5 I 2 /β-Bi 2 O 3 nanoflowers. Theoretical and experimental results reveal that FL-Bi 4 O 5 I 2 /δ-Bi 2 O 3 possesses not only improved photogenerated carrier separation, enhanced light harvesting, but also considerable CO 2 adsorption. Importantly, FL-Bi 4 O 5 I 2 / β -Bi 2 O 3 achieves S-scheme CO 2 conversion due to its reasonable energy band structure, and with the CO generation rate of up to 31.74 μmol g −1 h −1 under simulated sunlight.
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ANALYST
·
2023
·
IF 4.2
Zhaohong Su,Shiyu Hu,Yuhang Zhang,Zhanning Liang,Yi Peng,Qinyi Cao,Xia Yu,Zhiyang Zhu,Pei He,Zhenjie Li
Herein, the electrodeposition of paracetamol oxide (PA ox) for the intelligent portable ratiometric detection of nicotine (NIC) and ethyl vanillin β-D-glucoside (EVG) is reported. PA ox electrodeposited on a screen-printed carbon electrode (SPCE) was used as a new fixed state ratiometric reference probe. A portable electrochemical workstation combined with a smart phone was applied as an intelligent portable electrochemical sensing platform. The sensor was studied by scanning electron microscopy (SEM), Fourier transform infrared spectrophotometry (FT-IR), ultraviolet-visible spectrophotometry (UV-vis), theoretical calculation, chronoamperometry, cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and square wave voltammetry (SWV). Under optimized conditions, the detection range of NIC is 10–200 μmol L−1, and the detection limit is 0.256 μmol L−1. The detection range of EVG was 10–180 μmol L−1, and the detection limit was 0.058 μmol L−1. The sensor can realize the real-time detection of NIC and EVG concentration in cigarette samples quickly and accurately, and has good anti-interference, repeatability and stability.
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Pharmaceutics
·
2023
·Vol. 15
·期 12
·2684
·
IF 5.4
Yuanfan He,Jiani Xiang,Jie Chen,Sheng Fang,Zili Guo,Xianrui Liang
Chickpea isoflavones have diverse pharmacological activities but with low water solubility and bioavailability. In this work, the isoflavone content in chickpeas was first increased by germination, and then the bioaccessibility and bioavailability of isoflavones in chickpea sprout extracts (CSE) were enhanced using β-cyclodextrin (β-CD) inclusion techniques. Firstly, the total content of isoflavones was increased by 182 times through sprouting, and isoflavones were presented mostly in the germ and radicle. Then, the chickpea sprout extract/β-cyclodextrin (CSE/β-CD) inclusion complex was prepared and characterized. The in vitro test showed that the cumulative release of two isoflavones, formononetin (FMN) and biochanin A (BCA), in the CSE/β-CD was significantly increased in a simulated digestive fluid. The in vivo rat pharmacokinetics demonstrated that the inclusion of FMN and BCA by β-CD effectively increased their bioavailability in rat plasma and tissues, especially in the liver. The study provides a feasible strategy for improving the bioavailability of isoflavones from chickpeas and is also beneficial to the utilization of other legume resources.Keywords:chickpea isoflavones;cyclodextrin inclusion;pharmacokinetics
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BIOORGANIC & MEDICINAL CHEMISTRY
·
2016
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IF 2.923
Shenqi Wei,Wei Chen,Jingfang Qin,Yingzi Huangli,Li Wang,Yue Shen,Huang Tang
A series of 8- and 11-substituted oxoisoaporphine derivatives have been designed, synthesized, and tested for their ability to inhibit cholinesterase (ChE) in vitro and in vivo, and self-induced β-amyloid (Aβ) aggregation. Their autophagy activity and blood–brain barrier (BBB) permeability were also assessed. The new derivatives exhibited high AChE inhibitory activity in vivo and in intro. Over half the derivatives exhibited a significant in vitro inhibitory activity toward the self-induced Aβ aggregation. While, treatment of SH-SY5Y cells overexpressing the Swedish mutant form of human β-amyloid precursor protein (APPsw) with derivatives was associated with significant reduction of Aβ secretion levels. Moreover, one-third of the synthetic compounds were predicted to be able to cross the BBB to reach their targets in the central nervous system (CNS) according to a parallel artificial membrane permeation assay for BBB. Compounds 5b and 6b were chosen for assessing their autophagy activity. The fluorescence intensity of the BC12921 was decreased significantly after treatment with compounds. The result encourages us to study such compounds thoroughly and systematically.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Dongdong Zeng,Yutian Zhang,Xiaojun Ma,Jianing Li,Fen Yin,Dongna Li,Wenxuan Bie
Co-fermentation of multiple substrates has emerged as the most effective method to improve the yield of bioproducts. Herein, sustainable rubberwood enzymatic hydrolysates (RWH) were co -fermented by Aureobasidium pullulans to produce poly(β-L-malic acid) (PMA), and RWH + glucose/xylose was also investigated as co-substrates. Owing to low inhibitor concentration and abundant natural nitrogen source content of RWH, a high PMA yield of 0.45 g/g and a productivity of 0.32 g/L/h were obtained by RWH substrate fermentation. After optimization, PMA yields following the fermentation of RWH + glucose and RWH + xylose reached 59.92 g/L and 53.71 g/L, respectively, which were 52 % and 36 % higher than that after the fermentation of RWH. RWH + glucose more significantly affected the correlation between PMA yield and substrate concentration than RWH + xylose. The results demonstrated that the co-fermentation of RWH co-substrate is a promising method for the synthesis of bioproducts.
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Journal of Materials Research and Technology-JMR&T
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2023
·
IF 6.4
Bo Dong,Chengji Deng,Jun Ding,Hongxi Zhu,Qian Wang,Yong Zhang,Chao Yu
In this investigation, we have employed a novel approach to fabricate β–Sialon/Ti(C, N) composites through the nitridation treatment of Ti 3 SiC 2 powder. At temperatures ranging from 1373 to 1773 K, the reaction process was subjected to a comprehensive analysis to explore the transformation of phase and microstructure. The findings unequivocally highlight the influence of α–Al 2 O 3 presence and the high specific surface area of Ti 3 SiC 2 powder, resulting in distinctive phase and structural evolutions post nitridation, distinctly different from those observed in bulk Ti 3 SiC 2 . The β–Sialon/Ti(C, N) composites produced via this innovative method exhibit uniform local chemical composition and meticulous regulation of grain morphology. Moreover, the synthesis mechanism of β–Sialon/Ti(C, N) composites under nitrogen ambiance was thoroughly discussed. This straightforward approach has the potential for extensive–scale synthesis of β–Sialon/Ti(C, N) composites, offering promising avenues for their application in developing high–performance refractories or structural ceramics tailored to withstand severe high–temperature environments.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Yufan Huang,Jiaofen Lin,Bihua Shen,Chenmin Zheng,Bingqing Huang,Jinmei Zou,Guoguang Zhang,Peng Fei
In this study, chlorogenic acid-chitosan (CA-CS) copolymers were prepared with varying Chitosan (CS): chlorogenic acid (CA)ratios and characterized for their water solubility, antioxidant capacity, and emulsions stability. Results showed that CA-CS samples exhibited up to 90.5 % increase in DPPH scavenging efficiency and 20 % increase in hydroxyl radical scavenging efficiency compared to CS alone. CA-CS copolymers used to stabilize oil in water (O/W) emulsions, which were evaluated for their potential in encapsulating and protecting β-carotene. Microscopic observations revealed homogeneous spherical droplets in stable emulsions, suggesting effective interfacial structures. The selected CA-CS-stabilized O/W emulsions demonstrated encapsulation efficiencies of 74.8 % and 75.26 % for β-carotene. The CA-CS stabilized O/W emulsions provided the most effective protection against β-carotene degradation under UV exposure, retaining over 80 % of β-carotene content after 12 h of testing. These findings indicate that CA-CS-based O/W emulsions show promise as carriers and protectors for bioactive compounds, due to their improved antioxidant capacity, emulsions stability, and protection against degradation.
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