ACTA PHARMACOLOGICA SINICA
·
2024
·
IF 6.9
Hu You-tian,Liu Zhi-wei,Zhang Tong-hui,Ma Yu-e,He Lei,Zhang Jie,Zhou Yue-yang,Vidal-Puig Antonio,Pan De-jing,Wu Fang
Thymic egress is a crucial process for thymocyte maturation, strictly regulated by sphingosine-1-phosphate lyase (S1PL). Recently, cystathionine γ-lyase (CSE), one of the enzymes producing hydrogen sulfide (H 2 S), has emerged as a vital immune process regulator. However, the molecular connection between CSE, H 2 S and thymic egress remains largely unexplored. In this study, we investigated the regulatory function of CSE in the thymic egress of immune cells. We showed that genetic knockout of CSE or pharmacological inhibition by CSE enzyme inhibitor NSC4056 or D , L -propargylglycine (PAG) significantly enhanced the migration of mature lymphocytes and monocytes from the thymus to the peripheral blood, and this redistribution effect could be reversed by treatment with NaHS, an exogenous donor of H 2 S. In addition, the CSE-generated H 2 S significantly increased the levels of S1P in the peripheral blood, thymus and spleen of mice, suppressed the production of proinflammatory cytokines and rescued pathogen-induced sepsis in cells and in vivo. Notably, H 2 S or polysulfide inhibited S1PL activity in cells and an in vitro purified enzyme assay. We found that this inhibition relied on a newly identified C 203 XC 205 redox motif adjacent to the enzyme’s active site, shedding light on the biochemical mechanism of S1PL regulation. In conclusion, this study uncovers a new function and mechanism for CSE-derived H 2 S in thymic egress and provides a potential drug target for treating S1P-related immune diseases.
✓ 已验证
DOI
PubMed
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 3
·420
·
IF 3.06
Bang-Jin Wang,Ai-Hong Duan,Jun-Hui Zhang,Sheng-Ming Xie,Qiu-E Cao,Li-Ming Yuan
Porous organic cages (POCs) have attracted extensive attention due to their unique structures and tremendous application potential in numerous areas. In this study, an enantioselective potentiometric sensor composed of a polyvinyl chloride (PVC) membrane electrode modified with CC3-R POC material was used for the recognition of enantiomers of 2-amino-1-butanol. After optimisation, the developed sensor exhibited enantioselectivity towardS-2-amino-1-butanol (logKS,RPot= −0.98) with acceptable sensitivity, and a near-Nernstian response of 25.8 ± 0.3 mV/decade within a pH range of 6.0–9.0.Keywords:enantioselective potentiometric sensor;2-amino-1-butanol;chiral porous organic cage;CC3-R;PVC membrane electrode
✓ 已验证
DOI
PubMed
高影响因子
ACS Synthetic Biology
·
2025
·
IF 3.9
Hong-Rui Li,Ying Zhou,Bang-Ce Ye
Bacteriophages, leveraging phage display and chemical modification, have the potential to deliver large payloads of antitumor agents with precision and to advance vaccine development. However, systemic phage administration often induces neutralizing antibodies, which accelerate phage clearance and reduce accumulation at the target site. To address this limitation, we propose a genetically modified nonpathogenic bacterial strain that specifically targets tumors and releases programmed death ligand 1 (PD-L1)-specific M13 bacteriophage within tumor tissue. We assessed the antitumor efficacy of this phage-expressing strain as an adjunctive therapeutic strategy along with a therapeutic bacterial strain engineered for the controlled release of an immunotoxin. The combination of these strains demonstrated synergistic effects in eliciting antitumor immune responses and inhibiting tumor growth in a murine model of colorectal cancer (CRC). Moreover, when combined with Folfox, the phage-expressing strain significantly extended the survival. This strategy of in vivo expression and tumor-specific release mediated by nonpathogenic bacterial strains provides an effective and safe method for targeted therapeutic phage delivery to tumors.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2014
·
IF 3.916
Yajuan Zhang,Airong Xu,Benlian Lu,Zhiyong Li,Jianji Wang
The development of highly efficient cellulose solvents is imperative to the effective utilization of cellulose. In this work, ionic liquids (ILs) with the same 1-allyl-3-methylimidazolium cation ([Amim] + ) but different carboxylate anions, such as formate ([HCOO] − ), acetate ([CH 3 COO] − ), propionate ([CH 3 CH 2 COO] − ), butyrate ([CH 3 CH 2 CH 2 COO] − ), glycollate ([HOCH2COO] − ), lactate ([CH 3 CHOHCOO] − ) and benzoate ([C 6 H 5 COO] − ) were synthesized, and their thermal properties and viscosities were determined. Then these ILs were used to investigate the effect of anion structure on solubility of cellulose in the ILs. It was shown that the viscosity and cellulose solubility depended strongly on the anion structure of the ILs. For example, at 30 °C solubility of cellulose in [Amim][CH 3 CH 2 COO] was as high as 19.0%, whereas cellulose was not soluble in [Amim][HOCH 2 COO], [Amim][CH 3 CHOHCOO] and [Amim][C 6 H 5 COO]. In addition, solvatochromic UV/vis probe and 13 C NMR measurements were performed to demonstrate dissolution mechanism of cellulose in the ILs. The results suggested that although cations of the ILs have un-negligible contribution to the highly efficient dissolution of cellulose, hydrogen bonding interactions of anions of the ILs with cellulose is predominant.
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Zhen Sun,Yanqing Lai,Na lv,Yaqi Hu,Bingqin Li,Liangxing Jiang,Jiong Wang,Shuo Yin,Kui Li,Fangyang Liu
Argyrodite sulfide solid electrolytes, such as Li6PS5Cl (LPSC), have received much attention due to their high ionic conductivity (>1 mS cm–1) and success in all-solid-state batteries (long cycle performance, high energy density, etc.). Numerous efforts are spent on modifying the properties of the electrolyte itself. Here, we combine first-principles calculations with experiments to investigate O-doped argyrodite sulfide solid electrolytes (Li6PS5–xClOx,x = 0–1). It is found that Li6PS4.75ClO0.25 (LPSCO0.25) with x = 0.25 and cubic phase (F4̅3 m) shows the highest ion conductivity of 4.7 mS cm–1 (cold-pressed), higher than that of undoped Li6PS5Cl (4.2 mS cm–1). The bare LiCoO2/LPSCO0.25/Li–In all-solid-state battery exhibits an initial capacity of 131 mA h g–1 at 0.1 C and satisfactory cycling stability with 86% capacity retention after 250 cycles to the 4th cycle at 0.3 C under 25 °C. In addition, the NCM811/LPSCO0.25/Li–In cell is assembled using bare LiNi0.83Co0.06Mn0.11O2 cathode and shows an initial discharge capacity of 181 mA h g–1 at 0.1 C and 160 mA h g–1 at 0.3 C. The doping of oxygen-forming Li6PS5–xClOx also improves the stability to Li metal, proven by cyclic voltammetry and powder X-ray diffraction tests. The calculation results for the band structure reveals that LPSC has the lowest unoccupied molecular orbital than LPSCO0.25, further confirming the above conclusion.
✓ 已验证
DOI
高影响因子
ACS Applied Energy Materials
·
2023
·
IF 6.4
Lixin Chen,Xiaorui Yu,Zhe Hua,Qing Liu,Vladimir An,Lizhi Feng,Jiaming Guo,Xinglai Zhang,Jing Li,Baodan Liu
Photoelectrochemical water splitting based on suitable catalysts has attracted wide attention as a promising strategy to utilize solar energy to produce clean and renewable hydrogen fuel. Herein, we reported cobalt phosphate-modified (GaN)1–x(ZnO)x/GaN nanowire arrays on a high-temperature conductive GaN substrate toward enhanced photoelectrochemical water splitting by a facile two-step Au-assisted chemical vapor deposition method. The highly conductive Si-doped GaN substrate is designed to serve as a current collector and epitaxial substrate to grow GaN nanowires at high temperature. Meanwhile, high density of branched (GaN)1–x(ZnO)x nanowires with a tunable band gap, strong visible-light absorption, and high catalytic activity is deposited on the surface of GaN nanowires to act as active components to harvest light toward the oxygen evolution reaction. Such multi-junction heterostructures effectively enhance wide spectral utilization and light absorption and simultaneously accelerate the separation of electrons and holes and the transfer of photogenerated electrons from (GaN)1–x(ZnO)x nanowires to the GaN substrate collector and Pt electrode. The photocurrent density of the (GaN)1–x(ZnO)x/GaN nanowire array photoanode can reach 37.5 μA cm–2 at 1.23 V vs reversible hydrogen electrode and could be further enhanced to 186 μA cm–2 by modifying the cobalt phosphate cocatalyst, showing promising potential in clean hydrogen production.
✓ 已验证
DOI
高影响因子
JOURNAL OF POWER SOURCES
·
2016
·
IF 6.333
Lingchao Pan,Yonggao Xia,Bao Qiu,Hu Zhao,Haocheng Guo,Kai Jia,Qingwen Gu,Zhaoping Liu
Migration of transition metal (TM) ions to tetrahedral sites plays a crucial role on structural transformation and electrochemical behaviors for Li-rich layered oxides . Here, incorporating small B 3+ in the tetrahedral interstice is employed to block the migration channel of TM ions and stabilize the crystal structure. Benefiting from their good structural stability, Li-rich layered materials with B-doped Li 1.198 Ni 0.129 Co 0.129 Mn 0.535 B 0.01 O 2 and Li 1.196 Ni 0.127 Co 0.127 Mn 0.529 B 0.02 O 2 , exhibit excellent cycling performance and voltage stability. After 51 cycles at 0.2 C, 1 mol.% boron incorporated sample can deliver 211 mAh g −1 with capacity retention of 89.9%, which is much higher than that of the undoped sample of 177 mAh g −1 with the retention of 79.2%. Moreover, the declined voltage per cycle decreases from 3.6885 mV to 2.7530 mV after 2 mol.% boron doping. XRD patterns after extended cycling verified the suppression of the structural transformation by the incorporation of boron.
✓ 已验证
DOI
高影响因子
JOURNAL OF POLYMER SCIENCE
·
2022
·Vol. 60
·期 15
·2298-2308
·
IF 3.046
Zhaolai Huang,Jiaqi Li,Mengxiao Zhang,Weilin Feng,Chuanjie Fang,Liping Zhu
Polymers of intrinsic microporosity (PIMs) are promising materials for membrane separation because their special rigid and contorted structures contribute to high permeability. However, their chain rearrangement to fill excessive free volume makes the permeability stability a tough challenge. In this work, we report on a new use of rutile nano-TiO 2 to mitigate the physical aging of PIM-1 (a typical PIM) nanofilms for stable permeability by mixing matrix. It was shown that the PIM-1 membrane incorporated with nano-TiO 2 displayed remarkably higher aging resistance with a lower swelling degree in long-term ethanol soaking, having more stable ethanol permeance with only a 5% decrease after 35 days, lower than 25% of the pure one. The mechanism of anti-aging was revealed by molecular simulation, thermal, tensile mechanical, and dynamic mechanical analysis. It was found that nano-TiO 2 had good compatibility with PIM-1 due to strong coordination interaction, making its uniform dispersion in polymer. Additional solvent permeation channels were also created to increase solvent permeance without compromising solute rejection. Due to the reliable interaction of nano-TiO 2 , which makes particles serve as physical crosslinking points, the movement of PIM-1 chains was limited partially to mitigate aging, enabling PIM-1-based membranes to have robust solvent permeation. Graphical
✓ 已验证
DOI
高影响因子
ELECTROPHORESIS
·
2018
·Vol. 39
·期 22
·2912-2918
·
IF 2.569
Deying Kong,Zilin Chen
Because of unique properties like permanent porosity, low density and large surface area, covalent organic frameworks are attractive microporous materials as stationary phase for CEC. Among them, COF-TpPa-1 possesses extraordinary chemical stability and common features of covalent organic frameworks. So, COF-TpPa-1 could be a promising material as stationary phase to enhance separation selectivity and tolerate the acid or alkaline electrochromatographic separation conditions. Here, we report the preparation of COF-TpPa-1 modified capillary column by in situ growth for electrochromatographic separation. The immobilization of COF-TpPa-1 on the inner wall of capillary column was confirmed by Fourier transform infrared spectroscopy, scanning electron microscopy and X-ray diffraction. The fabricated COF-TpPa-1 modified capillary column indicated good resolution for the separation of neutral analytes, nonsteroidal anti-inflammatory drugs and food additives in open-tubular CEC mode. Compared with bare capillary column, the COF-TpPa-1 modified capillary column improved separation selectivity remarkably for tested analytes, including hydrophobic, π–π and hydrogen bond interactions. Besides, the prepared capillary column showed excellent repeatability and stability. The intraday, interday and column-to-column relative standard deviations of migration time for neutral analytes were less than 3.54%. This work shows enamine-linked covalent organic frameworks have great potential as stationary phases in CEC.
✓ 已验证
DOI
高影响因子
COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING
·
2024
·Vol. 27
·期 13
·1919-1929
·
IF 1.6
Zhang Fei,Chu Mingliang,Liu Jiemin,Zhao Qi,Zhu Yanqiu,Wu Xuefang
Aim: The study aimed to study the potential roles and mechanisms of shikonin in gastric cancer by network pharmacology and biological experiments. Methods: The key genes and targets of shikonin in gastric cancer were predicted by network pharmacology and molecular docking study.
The effect of shikonin on the proliferation, migration, and invasion of gastric cancer cells was detected by the CCK8 method, and wound healing and transwell assays. The expression levels of c-Myc and Yap-1 were detected via western blotting in gastric cancer cells after shikonin intervention.
Results: The results of network pharmacology revealed the key target genes of shikonin on gastric cancer cells to be c-Myc, Yap-1, AKT1, etc. GO and KEGG analysis showed regulation of cell migration, proliferation, adhesion, and other biological processes, including the PI3K-Akt signaling
pathway, HIF-1 signaling pathway, necroptosis, and other cancer pathways. Molecular docking showed shikonin to be most closely combined with protooncogenes c-Myc and Yap-1. In vitro experiments showed that the proliferation rate, migration, and invasion ability of the gastric cancer cell group
decreased significantly after shikonin intervention for 24h. The expression levels of c-Myc and Yap-1 in gastric cancer cells were found to be significantly decreased after shikonin intervention. Conclusion: This study showed protooncogenes c-Myc and Yap-1 to be the core target genes
of shikonin on gastric cancer cells. Shikonin may suppress gastric cancer cells by inhibiting the protooncogenes c-Myc and Yap-1. This suggests that shikonin may be a good candidate for the treatment of gastric cancer.
✓ 已验证
DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Zhengshan Gao,Xiang Liu,Chengli Zhang,Zhiyong Tang,Jun Chen,Chao Yu
A novel label-free electrochemical sensing strategy for detecting monocyte chemoattractant protein-1 (MCP-1) was developed by using single-walled carbon nanohorns (SWCNHs) functionalized with Pt nanoparticles (PtNPs-SWCNHs). After modification with the PtNPs-SWCNHs, the electrode could serve as an effective platform for antibody immobilization and electron transfer owing to the large surface area and excellent conductivity of PtNPs-SWCNHs. Moreover, the PtNPs-SWCNHs composite could provide an obvious electrochemical signal through amperometric i–t curve method, due to its high catalytic activity for the reduction of hydrogen peroxide (H 2 O 2 ). Under optimal conditions, the amperometric i-t curve signals had a strong linear relation with the logarithm of the MCP-1 concentration ranging from 0.06 pg mL -1 to 450 pg mL -1 , and the detection limit was 0.02 pg mL -1 . Furthermore, the proposed immunosensor could detect MCP-1 in serum samples with desirable results. Overall, this immunosensor may provide a new alternative strategy for rapid detection of MCP-1.
✓ 已验证
DOI
高影响因子
Chemical Engineering Journal Advances
·
2022
·
IF 0
Hiu Lam So,Wei Chu
Degradation of 1-Naphthol by combined photocatalysis and adsorption in column reactor was studied, and the performance could be predicted by employing a proposed multiple regression model. The model includes four variables: concentration of 1-Naphthol, concentration of peroxymonosulfate (PMS), FeOOH dosage and pH, along with their second-order effects and interactions. Statistical analyses were performed to verify the statistical significance of the regressors, and extra experimental sets were conducted to validate the proposed model. The mathematical model proposed exhibited good reproducibility with no significant statistical difference between the experimental and predicted values. Response surface methodology was used to explore the relationship between the four variables and the response. The process optimal ranges can be further refined at pH 2–2.5; [PMS] = 1.2–1.4 mM; and FeOOH 2.75–3 g respectively, according to the proposed overlaid contour plots.
✓ 已验证
DOI
危险品化学品经营许可证（带存储）