FEMS MICROBIOLOGY LETTERS
·
2018
·Vol. 365
·期 5
·
IF 1.735
Ke Xia,Xia Xiao-Yuan,Zheng Ren-Chao,Zheng Yu-Guo
The Erg28p protein is localized to the endoplasmic reticulum, where it acts as a scaffold to tether the C-4 demethylase complex involved in the sterol biosynthesis pathway of Saccharomyces cerevisiae. However, due to the challenges involved in characterizing the interactions of membrane proteins, the precise region of Erg28p that is responsible for the assembly of this enzyme complex remains unknown. To address this question, mutants with serial truncations in the C-terminus of Erg28p were constructed based on a topology prediction of its transmembrane domain. Sterol profiles demonstrated that intermediates involved in the stepwise removal of the two C-4 methyl groups from the tetracyclic sterol ring were accumulated in the ERG28Δ135-447 strain. Homologous alignment of Erg28p further identified a highly conserved 10-amino acid sequence (63LS/QARTFGT/LWT72) within the truncated region of ERG28Δ136-273. Complementation of the BY4741/erg28 strain with the ScERG28Δ175-204 plasmid resulted both in a significant growth inhibition and a reduction of ergosterol biosynthesis compared with the plasmid without the Δ175-204 truncation. Furthermore, homology modeling of the Erg28p mutant indicated that the deletion of residues 63–72 significantly disrupted the 3D structure of the four parallel helices in Erg28p. Taken together, the data indicate that the region spanning amino acids 63–72 constitutes a key consensus motif within Erg28p that is required for sterol C-4 demethylation during ergosterol biosynthesis in S. cerevisiae.
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MATERIALS CHEMISTRY AND PHYSICS
·
2018
·
IF 2.21
Jianwei Fu,Shaomin Wang,Jianhua Zhu,Kai Wang,Meng Gao,Xuzhe Wang,Qun Xu
Highly dispersed Au-Ag bimetallic nanoparticles were facilely synthesized on the multi-amino poly(cyclotriphosphazene-co-polyethyleneimine) (PCP) microspheres through in situ co-reduction method and then used as catalysts for the reduction of 4-nitrophenol (4-NP). Characterization results showed that the as-synthesized PCP microspheres possessed regular spherical morphology and relatively smooth surface, which could be well modified by highly dispersed Au-Ag bimetallic nanoparticles with size of 2–4 nm. For the reduction of 4-NP, it was found that the as-synthesized PCP@Au-Ag composites showed much better catalytic activity than PCP microspheres supported monometallic counterparts, which could be ascribed to a synergistic effect between Au and Ag in Au-Ag bimetals . For the PCP@Au-Ag catalysts, the rate constant k and intrinsic activity parameter κ of the reaction could be up to 2.87 × 10 −3 s −1 and 144 s −1 g −1 , respectively, and the conversion of the 4-NP could still reach 83% after six cycles. In addition, the possible catalytic mechanism of PCP@Au-Ag composites towards the reduction of 4-AP was also proposed.
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POLYMERS FOR ADVANCED TECHNOLOGIES
·
2018
·Vol. 29
·期 11
·2838-2847
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IF 2.137
Shuchen Shi,Dongyi Li,Chunxiao Chai,Yufeng Wu,Ying Xu
A novel modified polyaspartic acid polymer, polyaspartic acid/4-(2-aminoethyl) morpholine graft copolymer (PASP/AEM), was prepared through a ring-opening reaction of polysuccinimide and 4-(2-aminoethyl) morpholine and characterized by 1 H NMR. The scale inhibition performance of this copolymer and its ability to disperse Fe 2 O 3 were investigated in static tests. Scanning electron microscopy and X-ray powder diffraction were used to analyze the effects of PASP/AEM on scale formation. The performance and mechanism of corrosion inhibition by the synthesized copolymer were studied via weight loss measurements and electrochemical measurements. The results showed that PASP/AEM possessed outstanding scale and corrosion inhibition efficiencies and had potential for use as a green scale and pickling inhibitor.
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INTERNATIONAL IMMUNOPHARMACOLOGY
·
2019
·
IF 3.361
Sen Yang,Ge Kuang,Rong Jiang,Shengwang Wu,Tao Zeng,Yuanyuan Wang,Fangzhi Xu,Lingyi Xiong,Xia Gong,Jingyuan Wan
Acetaminophen (APAP) is a widely used over-the-counter drug for antipyretic and analgesic , but an overdose will induce acute liver injury. APAP hepatotoxicity has been the most common cause of acute liver failure in western countries with high morbidity and mortality. Geniposide (GP), an iridoid glycoside extracted from the fruit of Gardenia jasminoides , has been reported to exert a profound anti-inflammatory activity on acute and chronic diseases. However, it is never demonstrated whether GP can protect hepatocytes from APAP hepatotoxicity. In this study, we investigated the protective effect and underlying mechanism of GP against AILI. The results showed that GP pretreatment reduced the levels of ALT and AST in a dose-dependent manner and alleviated hepatocyte necrosis and apoptosis in mice exposed at APAP. Moreover, it suppressed the expression of CYP 2E1 and attenuated the exhaustion of GSH and accumulation of MDA in the liver. Furthermore, GP remarkably inhibited inflammatory cells infiltration and mitigated the release of IL-1β and TNF-α, and inhibited Toll-like receptor 4 (TLR4) expression and nuclear factor kappa (NF-κB) activation. These data suggested that GP could effectively protect hepatocytes from APAP hepatotoxicity through the down-regulation of CYP 2E1 expression and the inhibition of TLR4/NF-κB signaling pathway .
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Environmental Science-Nano
·
2019
·Vol. 6
·期 10
·2986-2999
·
IF 7.704
Yuanhong Zhong,Zhi-Feng Chen,Shi-Chao Yan,Wen-Wen Wei,Qianxin Zhang,Guoguang Liu,Zongwei Cai,Lin Yu
Climbazole (CBZ) is an emerging contaminant with adverse effects on aquatic organisms. In this study, a floral array of chromium-substituted magnetite nanoparticles was synthesized, characterized, and used in photocatalytic degradation of CBZ. The results showed that Cr incorporation did not change the spinel structure of magnetite, but resulted in the obvious decrease of its crystallinity. The Cr content greatly influenced the morphology and micro-structure of magnetite nanoparticles, which led to a significantly larger surface area, and more abundant pores and surface hydroxyl groups. Factors such as the UV light source, catalyst concentration, initial level of CBZ, solution pH, peroxymonosulfate (PMS) dosage, and Cr level in magnetite affected the transformation of CBZ. Under the optimized photocatalytic conditions, i.e., 0.2 g L−1 Fe2.52Cr0.48O4, 2.0 μmol L−1 CBZ, 1.0 mmol L−1 PMS, and pH = 3.08, CBZ was almost completely eliminated within 30 min, which was much faster than under photodegradation, biodegradation, or chlorination reported previously. The reactive oxygen species including ˙OH and SO4˙− coexisted and participated in the photocatalytic reaction. The GC-MS analysis revealed the generation of 4-chlorophenol (4-CP) as a primary intermediate following CBZ transformation, based on the stoichiometric relationship between CBZ and 4-CP. The resultant 4-CP in the present study does not increase the risk to aquatic life. The results highlight an environmentally friendly strategy for effective elimination of CBZ.
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TALANTA
·
2017
·
IF 4.162
Huijuan Wang,Siyu Zhang,Shufang Li,Jianying Qu
Herein, a newly developed electrochemical sensor base on the nanohybrid of palladium-reduced graphene oxide modified with gold nanoparticles (Au/Pd/rGO) was established, which was prepared by electrodeposing Au nanoparticles on Pd/rGO modified on a glass carbon electrode. The morphologies and microstructures of the as-prepared nanohybrid were characterized by X-ray photoelectron spectroscopy, Scanning electron microscopy and Infrared spectroscopy . And, experiment results showed that the prepared Au/Pd/rGO nanohybrid exhibited excellent electrocatalytic- activity toward the redox of acetaminophen (PA) and 4-aminophenol (4-AP) simultaneously. The linear detection ranges were 1.00–250.00 μM for PA and 1.00–300.00 μM for 4-AP, with the detection limits of 0.30 μM for PA and 0.12 μM for 4-AP, respectively. Because of the excellent performance of lower detection, wider linear range and better selectivity, the prepared Au/Pd/rGO nanohybrid with more potential applications was a promising candidate for advanced electrode material in electrochemical sensing field.
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JOURNAL OF ALLOYS AND COMPOUNDS
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2017
·
IF 3.133
Ying-Hua Zhou,Suqin Wang,Yu Wan,Jingjing Liang,Yu Chen,Shizhong Luo,Cheng Yong
Low-cost metallic nanoparticles as catalysts with good performance are highly desired for the development of hydrogen storage materials . In this work, ceria-doped CuNi nanoparticles were successfully immobilized on the surfaces of reduced graphene oxide (rGO) by a simple co-reduction approach in aqueous solution at room temperature, characterized by ICP-OES, XRD , TEM, SEM-EDS and XPS techniques. The as-synthesized CuNi-CeO 2 /rGO nanocomposites with various metal contents had been employed as heterogeneous catalysts for the hydrolytic dehydrogenation of ammonia borane (NH 3 BH 3 , AB) under mild conditions. The optimal catalyst of Cu 0.8 Ni 0.2 -CeO 2 /rGO with CeO 2 content of 13.9 mol% exhibited an excellent catalytic activity with the exceedingly high TOF value of 34.4·mol H2 ·mol catalyst −1 ·min −1 at 298 K. The apparent activation energy was calculated to be 19.1 kJ mol −1 , even lower than most of noble-metal-based catalysts for AB dehydrogenation. The detailed kinetics of AB hydrolysis catalyzed by Cu 0.8 Ni 0.2 -CeO 2 /rGO had been investigated on different concentrations of catalyst, substrate and temperatures. Moreover, the obtained in situ hydrogen from AB hydrolysis can be further applied to reduce 4-nitrophenol with a high TOF value of 8.1 min −1 at room temperature. The high catalytic activity of Cu 0.8 Ni 0.2 -CeO 2 /rGO would be contributed to the small size of CuNi-CeO 2 NPs, metal-metal synergy, and the metal-support interaction.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Nuo Chen,Zhiyuan Xing,Hongying Liu,Lu Fang,Yuqing Zhang,Lihua Li,Zheng-Zhi Yin,Maofeng Wang
Reduced nicotinamide adenine dinucleotide (NADH) plays crucial roles in maintaining cell growth and differentiation, cytoprotection and energy metabolism. And it is imperative to precisely monitor the variable quantity of NADH. Herein, the connotative feature of acupuncture needle (AN) was explored as electrochemical matrix. Poly-3, 4-ethylenedioxythiophene-liganded gold nanoparticle modified AN electrode (ANE/AuNPs/PEDOT) was prepared via a simple electrosynthesis technology. Working together by charge transfer and reaction has a strong response to the electron-transfer of “N” in NADH, which is coordinately presented as a current peak. Interestingly, the behavior of electron-transfer was affected by ambient environment. After the conditional optimization, the fabricated sensor exhibited good linear range of 20 ∼ 500 μmol/L with a detection limit of 18.5 μmol/L. In addition, the sensor can be successfully applied to analysis NADH in serum. Furthermore, the microelectrode exhibits the real-time monitor ability for NADH that released from MDA-MB231 cells. This sensor also possesses the significant performance of high selectivity, stability and reproducibility. Based on the acupuncture history, the developed ANE should be a fascinating microsensor besides the low price, fast response and user-friendly operation.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Taotao Zhao,Chenyang Shen,Gui Liu,Jun Yao,Xianghao Wang,Huajie Tao,Yu He,Yuchi Liu,Zhewei Zhang,Feifei Mei,Xiangke Guo,Luming Peng,Yan Zhu,Xuefeng Guo,Nianhua Xue,Weiping Ding
We report here a high-performance catalyst ReMoS x @HSSZ-39 compounded with SAPO-34 for conversion of CO 2 to C 2-4 hydrocarbons, in which the ReMoS x clusters are responsible for highly efficient hydrogenation of CO 2 to methanol as the intermediate and then the surrounding zeolitic acid sites for further conversion of methanol to hydrocarbons. The investigation finds that uniform ReMoS x clusters can be reliably fabricated in zeolitic cages by co-introduction of rhenium and molybdenum species into the HSSZ-39 zeolite and followed by sulfidation, which constitutes an intimate bifunction catalyst through better cooperation of ReMoS x clusters and the zeolitic acid sites. Compared with MoS x @HSSZ-39, the introduction of rhenium significantly suppresses CO generation. By mixing some SAPO-34 zeolite, with increased density of protonic acid, more than 86 % C 2-4 hydrocarbons in organic products and only 21 % CO at 32.5 % of CO 2 conversion are obtained and the composite catalyst shows excellent stability with more than 95 % C 2-4 hydrocarbons in organic products, less than 30 % CO and above 26.6 % of CO 2 conversion after 1000 h on stream.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2024
·
IF 8.1
Zhaoyan Deng,Yuhan Chen,Qiang Tian,Tianyi Guo,Yanjuan Zhang,Zuqiang Huang,Huayu Hu,Tao Gan
Modulation of the electronic structure of heterogeneous catalysts by introducing heterojunctions is an effective means to improve their catalytic performance. Although Co-based nanocatalysts have been widely used in the reduction of 4-nitrophenol (4-NP) wastewater, Co single-atom catalysts (SACs) have rarely been reported. Herein, we successfully fabricate Co SACs supported on CNT/MoS 2 heterojunction nanocomposite (Co 1 -MoS 2 /CNT) by a hydrothermal synthesis combined with the subsequent impregnation method. The catalytic performance of Co 1 -MoS 2 /CNT is evaluated using the reduction of 4-NP in an aqueous media containing sodium borohydride. Complete reduction of 4-NP catalyzed by Co1-MoS 2 /CNT occurs within 5 min at room temperature, and the apparent rate constant k can reach 1.16 min −1 , which is 4.1 and 38.6 times higher than those of Co 1 -CNT and Co 1 -MoS 2 , respectively. The improved catalytic performance of Co 1 -MoS 2 /CNT is attributed to the p-n heterojunctions of MoS 2 /CNT increasing the electron density of the Co single-atom sites, as well as the CNTs with excellent conductivity, accelerating the electron transfer. Moreover, Co 1 -MoS 2 /CNT exhibits excellent stability and favorable applicability, endowing its potential for practical applications. Our findings not only provide useful insights into the enhancement of catalytic performance of SACs, but also open a new avenue for the treatment of 4-NP wastewater.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2016
·
IF 2.76
Yu Zheng,Ping Zhang,Hairong Yue,Gang Xiang,Zexin Qian,Hongtai Li,Wei Jiang,Bin Liang,Simo O. Pehkonen,Shaojun Yuan
Ni 3 Si 2 O 5 (OH) 4 nanotubes (NTs) are synthesized by a facile one-pot hydrothermal reaction, and further functionalized by grafting of poly(methacrylic acid) (PMAA) brushes via distillation-precipitation polymerization. The resultant PMAA-grafted NTs are used as a novel nanosorbent to uptake copper(II) ions. Success in each functionalization step is ascertained by the characterization of TEM, FTIR, XPS, SEM and TGA. Batch adsorption results indicate that the adsorption of the pristine and PMAA-grafted silicate NTs is fairly fast and the equilibrium is established within 20 min, and the kinetics follow the pseudo-second-order mechanism, evidencing chemisorption as the rate-limiting step. The best interpretation for the equilibrium data is given by Langmuir isotherm, and the maximum adsorption capacity is 2.13 mmol g −1 (136 mg g −1 ) at pH 5 and 25 °C. The successive adsorption-desorption studies reveal that the PMAA-grafted silicate NTs are readily regenerated, and keep their adsorption and desorption efficiencies constant over five cycles. The Ni 3 Si 2 O 5 (OH) 4 - g -PMAA NTs could be directly reduced via hydrothermal reaction to achieve magnetic property for easy separation. With the inherent advantages of pH-sensitivity of PMAA, large surface area and magnetic properties of Ni 3 Si 2 O 5 (OH) 4 NTs, the PMAA-grafted silicate NTs offer a promising and highly efficient nanocomposite sorbent to remove heavy metal ions from aqueous solution.
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TALANTA
·
2016
·
IF 4.035
Zhuomin Zhang,Cheng Zhao,Gongke Li
Achieving reproducible signals is a key point to improve the analytical precision and accuracy of surface enhanced Raman scattering (SERS) technique and further expand the application scope of SERS for on-site and rapid analysis of real sample with complex matrice. In this work, a novel Au@hydroxyl-functionalized polystyrene (Au@PS-OH) substrate was prepared by atom transfer radical polymerization and chemical assembly method, which possessed promised potential for the rapid and sequential analysis of multisamples coupling with SERS technique. Au@PS-OH substrate with regular nanoarrayed morphology possessed excellent anti-agglomeration capability even for testing solutions with strong basicity or acidity, mechanic and chemical stability due to the large amount of Au nanoparticles homogeneously and stably fixed on substrate surface. Moreover, excellent hydrophobicity of Au@PS-OH substrate could keep testing droplets of multiple samples stable and uniform spherical shape with similar contact angles to substrate, which guaranteed the reproducible SERS light paths and SERS signals during real sequential analysis. Then, an Au@PS-OH based SERS analytical method was developed and practically applied for the sequential determination of trace 4-aminoazobenzene in various textiles. It was satisfactory that the contents of trace 4-aminoazobenzene in black woolen, green woolen and yellow fiber cloth could be actually found and calculated to be 106.4, 120.9 and 140.8 mg/kg with good recoveries of 76.0-118.9% and relative standard deviations of 1.6-5.1%. It is expected that this SERS method is suitable for on-site and rapid analysis of multiple samples in a short period.
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