Biomedical Engineering Online
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2021
·Vol. 20
·期 1
·1-13
·
IF 2.819
Ge Jinlin,Yang Huanhuan,Zeng Yufeng,Liu Yunjie
Background Wogonin (5, 7-dihydroxy-8-methoxyflavone) is a natural di-hydroxyl flavonoid extracted from the root of Scutellaria baicalensis Georgi. This paper was intended to investigate the mechanism of action of wogonin in alleviating the inflammation and apoptosis in acute lung injury (ALI).Materials and methodsLipopolysaccharide (LPS) was used to establish the in vitro model of ALI. After wogonin treatment, the cell viability and apoptosis of LPS-induced A549 cells were, respectively, measured by CCK-8, TUNEL assays and acridine orange/ethidium bromide dual staining, while the contents of inflammatory cytokines and oxidative stress markers were estimated by RT-qPCR, ELISA assay, western blot analysis and commercial kits. Western blot was also conducted to assess the expression of proteins involved. Subsequently, the effect of wogonin on the sirtuin 1 (SIRT1)-mediated high-mobility group box 1 protein (HMGB1) deacetylation was investigated. SIRT1 inhibitor EX527 was used to evaluate the regulatory effects of wogonin on SIRT1-mediated HMGB1 deacetylation in A549 cells under LPS stimulation.Results LPS induced inflammation, oxidative stress and apoptosis of A549 cells, which was abolished by wogonin. It was also found that wogonin promoted the HMGB1 deacetylation, accompanied by upregulated SIRT1 expression. However, SIRT1 inhibitor EX527 partially reversed the protective effects of wogonin on the inflammation and apoptosis of LPS-induced A549 cells.Conclusion Wogonin alleviated the inflammation and apoptosis in LPS-induced A549 cells by SIRT1-mediated HMGB1 deacetylation, which might represent the identification of a novel mechanism by which wogonin exerts protective effects on ALI and provide ideas for the application of wogonin to ALI treatment.
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Parasites & Vectors
·
2021
·Vol. 14
·期 1
·1-6
·
IF 3.876
Li Meilin,Liu Shunlong,Yin Zhijuan,Bernigaud Charlotte,Guillot Jacques,Fang Fang
Background The limited ovicidal activity of currently available acaricides is a significant obstacle to efficacious scabies treatment. Several essential oils or their respective components have proved to be active against the eggs of arthropods, mainly lice and ticks. Information on the activity of these oils and/or components against the eggs of mites remains very limited. The aim of this study was to assess the activity of six terpenes (carvacrol, eugenol, geraniol, citral, terpinen-4-ol and linalool) commonly found in essential oils against the eggs of Sarcoptes scabiei . Methods Sarcoptes eggs were exposed to paraffin oil containing 1, 2.5, or 5% of each terpene tested. After a 12-h exposure period, the eggs were washed and placed in paraffin oil for hatching. Embryonic development following treatment was assessed every day to determine the stage of developmental arrest. Results The median effective concentration to obtain 50% egg mortality (EC 50 ) was 0.5, 0.9, 2.0, 4.8, 5.1 and 9.8% for carvacrol, eugenol, geraniol, citral, terpinen-4-ol and linalool, respectively. The microscopic images of eggs after each treatment indicated that these six terpenes may act by penetrating through the aeropyles on the egg surface. Conclusions In conclusion, carvacrol, eugenol and geraniol possess significant ovicidal activities, which should be considered as promising ovicidal agents for the treatment of scabies. Graphical Abstract
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 414
·期 4
·1631-1640
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IF 4.157
Qian Jingqi,Wang Mian,Wang Zhaoxiang,Feng Rui,Zhang Jiaqi,Ye Chencheng,Zhang Man,Wang Baomin,Cui Liwang
Amodiaquine (AQ) is a commonly used antimalarial drug, and N -desethyl-AQ (N-DEAQ) is an active metabolite of AQ. Given the significance of drug quality in the management of malaria cases, this study aims to develop antibody-based assays for the detection and quantitation of AQ without the need for sophisticated equipment. Two monoclonal antibodies (mAbs) against AQ, designated as JUN7 and TE7, were selected, which showed 72.7% and 9.5% cross-reactivity to N-DEAQ, respectively. These mAbs showed <0.1% cross-reactivity to other commonly used antimalarial drugs. An indirect competitive enzyme-linked immunosorbent assay (icELISA) based on JUN7 showed a 50% inhibitory concentration (IC 50 ) of 0.16 ng/mL and a working range of 0.06–0.46 ng/mL. A lateral flow immunoassay (LFIA) based on JUN7 was also developed with a working range of 2.58–30.86 ng/mL. The icELISA and LFIA were applied for the quantification of AQ in commercial drugs, and the results were comparable to those determined using high-performance liquid chromatography. In addition, a combination dipstick for simultaneous, qualitative analysis of AQ and artesunate was developed. All immunoassays based on JUN7 can be applied for quality control of AQ-containing artemisinin-based combination therapies. As TE7 showed low cross-reactivity to N-DEAQ, an icELISA based on TE7 was developed with an IC 50 of 0.38 ng/mL and a working range of 0.14–1.67 ng/mL. The TE7 icELISA was applied for the study of pharmacokinetics of AQ in rat serum after intragastric administration, and the results were consistent with those of previous studies. Graphical abstract
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CATALYSIS LETTERS
·
2021
·Vol. 152
·期 9
·2688-2694
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IF 3.186
Cai Mingzhong,Liu Rong,Xu Caifeng,Huang Bin
Pd(OAc) 2 /XPhos–SO 3 Na in a mixture of poly(ethylene glycol) (PEG-400) and water is shown to be a highly efficient catalyst for the cyanation of aryl chlorides with potassium ferrocyanide. The reaction proceeded smoothly at 100 or 120 ºC with K 2 CO 3 or KOAc as base, delivering a variety of aromatic nitriles in good to excellent yields. The isolation of the crude products is facilely performed by extraction with cyclohexane and more importantly, both expensive Pd(OAc) 2 and XPhos–SO 3 Na in PEG-400/H 2 O system could be easily recycled and reused at least six times without any apparent loss of catalytic efficiency. Graphical Abstract Palladium-catalyzed cyanation of aryl chlorides with potassium ferrocyanide leading to aryl nitriles by using Pd(OAc) 2 /XPhos–SO 3 Na/PEG-400/H 2 O as a highly efficient and recyclable catalytic system is described.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 29
·期 17
·25581-25590
·
IF 4.223
Li Haocong,Li Yong,Wang Wenfeng,Wan Qun,Yu Xiangyang,Sun Wenjing
Triazole pesticides are widely used for the control of pathogenic fungi in crops, which were frequently detected in edible parts. Its extensive use has caused many environmental pollution and food safety problems. In this study, the uptake, translocation, and subcellular distribution of three triazole pesticides (triadimefon, tebuconazole, and epoxiconazole) in rice were investigated. The results showed that the three triazole pesticides could be taken up by rice roots, but their distribution in plant tissues were different. The accumulation of the three pesticides in rice root followed the order of epoxiconazole (4.26 mg/kg, 24 h) > tebuconazole (2.63 mg/kg, 24 h) > triadimefon (1.37 mg/kg, 24 h), while a reversed order was observed in rice shoots, triadimefon (0.48 mg/kg, 24 h) > tebuconazole (0.40 mg/kg, 24 h) > epoxiconazole (0.21 mg/kg, 24 h). The translocation of triazole pesticides within rice tissues involved both symplast and apoplast pathways, with triadimefon preferentially through by the apoplast pathway and epoxiconazole through by the symplast pathway. The proportions of triadimefon, tebuconazole, and epoxiconazole in the symplast and apoplast of rice plants were 15–33%, 6–31%, 7–37%, and 67–85%, 69–94%, 63–93%, respectively. The subcellular distribution revealed that all pesticides have a higher proportion in cell walls than in cell organelles and soluble components. Epoxiconazole has the highest accumulated capacity in the cell wall (45–67%) and triadimefon was more concentrated in the soluble components (24–29%). However, there were no significant differences in the amount of three pesticides in cell organelles. The distribution of the three pesticides in aboveground and underground parts of rice plant, uptake and transportation in symplast and apoplast pathways, and distribution in the subcellular tissue are all related to their hydrophobicity.
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AAPS PHARMSCITECH
·
2021
·Vol. 23
·期 1
·1-13
·
IF 3.246
Wu Wenqing,Cao Wenxuan,Chen Jingbao,Cai Ye,Dong Baoqi,Chu Xiaoqin
The purpose of this study was to design an in situ liquid crystal gel (ISLG) as an ophthalmic drug delivery system for dexamethasone (DEX) to enhance its eye retention and ocular bioavailability. The in situ liquid crystal gels (ISLGs) were prepared using a phytantriol/PEG400/water (65:30:5, w/w) ternary system. Polarized light microscope (PLM), small-angle X-ray scattering (SAXS), and rheology analysis confirmed that the internal structure of the preparations was Pn3m cubic phase liquid crystal gels with pseudoplastic fluid properties. Meanwhile, in vitro release behavior of the preparations conforms to the Higuchi equation. Corneal penetration experiments showed that compared with DEX sodium phosphate eye drops, DEX-ISLGs(F2) produced a 5.45-fold increase in the P app value, indicating a significant enhancement of corneal penetration. In addition, in vivo experiments have confirmed that the ISLGs have better biocompatibility and longer retention time in the cornea. Simultaneously, corneal hydration level, eye irritation experiments, and histological observations proved the safety of the preparations. Pharmacokinetic studies have shown that the ISLG could maintain the DEX concentration in aqueous humor for at least 12 h after administration, which significantly improves the bioavailability of the drug. Collectively, these results indicated that ISLG would be a potential drug carrier for the treatment of diabetic retinopathy (DR).
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TRIBOLOGY LETTERS
·
2021
·Vol. 70
·期 1
·1-16
·
IF 3.106
Sheng Dezun,Yu Hongliang,Li Hongyue,Zhou Jinxi,Zhang Huichen,Wang Weiwei
In this paper, the coupling agent of APTES was employed to improve the lubricating properties of hydrated HEC. The synergistic effect between APTES and HEC was analyzed by means of Raman, FT-IR, and optical observations. The lubricating properties of water, HEC, APTES, and APTES–HEC solutions were separately investigated by the tribological tests with Si 3 N 4 ball and textured Ti–6Al–4V. It was found that APTES can promote the interaction between HEC molecules, resulting in the formation of homogeneous films without clustering, while thickening the solutions. The APTES-enhanced HEC exhibits excellent lubrication behavior with minimal coefficient of friction, which decreases from 0.029 to 0.017 (down by 38%) as friction goes on. In addition, by measuring the wear loss of the balls, it was shown that the mixture of APTES and HEC provided the best anti-wear capability. The film thickness calculated by the Dowson–Hamrock theory matches the trend of the friction coefficient. However, when the friction velocity exceeds a threshold value, the theory is not applicable anymore. The SEM images detected the presence of two structures in the wear area, namely plow and asperity, and suggested two corresponding lubrication states. The hydrodynamic lubrication occurs within the plow. XPS results suggest that APTES can form Si–O–Ti via chemical reaction, which induces the adsorption of APTES–HEC chains onto the asperities of worn Ti–6Al–4V. The adsorption of APTES–HEC produces an adsorbed lubrication layer, which prevents direct contact between the asperities where boundary lubrication occurs. Graphical Abstract
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Reaction Kinetics Mechanisms and Catalysis
·
2021
·Vol. 134
·期 2
·837-849
·
IF 2.081
Zhao Xinhong,Niu Li,Hao Zongyi,Long Xuefeng,Wang Dongliang,Li Guixian
SSZ-13 has been widely used in catalysis and adsorption due to its excellent hydrothermal stability and large specific surface area. It was reported that the long crystallization time was one of the primary challenges limiting its industrial progress. In this paper, we reported a novel solid-state grinding route of synthesizing SSZ-13 zeolite, which was based on interzeolite transformation strategy and promoted by sodium persulfate (Na 2 S 2 O 8 ). The effects of the amount of Na 2 S 2 O 8 and crystallization time on the purity and crystallinity of SSZ-13 zeolite were studied by XRD measurements in detail. The results indicated that the crystallization time could be significantly shortened from 72 to 24 h when the molar ratio of Na 2 S 2 O 8 /SiO 2 was changed from 0 to 0.01, proving that Na 2 S 2 O 8 has the ability of accelerating the crystallization of SSZ-13 as a hydroxyl radical reagent. Three typical SSZ-13 materials and a reference sample were used as the catalysts for methanol-to-olefins reaction (MTO) and their physicochemical properties were analyzed by SEM, N 2 physisorption and NH 3 –TPD techniques. The SEM characterization results showed that the crystal size of SSZ-13 synthesized in the presence of Na 2 S 2 O 8 was larger than that of the one in the absence of it. The NH 3 –TPD results showed that the introduction of Na 2 S 2 O 8 could modulate the acid properties of SSZ-13 zeolites. The MTO tests revealed that the catalytic lifetime of one sample with Na 2 S 2 O 8 promoter (SSZ-13-0.01M-24h) was much longer than that of the other three samples. The observed difference in catalytic stability was mainly attributed to their various concentrations of medium strong acid sites.
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Tungsten
·
2021
·Vol. 4
·期 2
·99-108
·
IF 0
Li Feng-Rui,Ji Tuo,Chen Wei-Lin
A purely inorganic tri-vanadium-capped Keggin phosphomolybdate [Na 3 PMo 12 V 3 O 43 ]·4H 2 O (compound 1 ) has been synthesized by hydrothermal means and characterized via powder X-ray diffraction, infrared spectrum, thermogravimetric analysis, single-crystal X-ray diffraction, scanning electron microscope, ultraviolet–visible absorption spectroscopy, ultraviolet–visible diffuse reflection spectra as well as electrochemical cyclic voltammetry tests. The structure analysis displays that compound 1 is a symmetric tri-vanadium-capped cluster through six half occupancy {VO 5 } units. Compound 1 remains a purely inorganic Keggin phosphomolybdate, which presents electrocatalytic properties for the oxidization of ascorbic acid and reduction of H 2 O 2 . At the same time, it possesses high-efficiency degradation capability for typical methylene blue, Rhodamine B and methyl orange dyes under ultraviolet light.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2021
·Vol. 32
·期 24
·28417-28425
·
IF 2.478
Wang Jinlin,Zhou Xiaojun,Ni Jian,Guan Jiayi,Hu Minghao,Wang Rufeng,Zhang Yaofang,Li Juan,Cai Hongkun,Zhang Jianjun
Mesoporous TiO 2 (m-TiO 2 ) is widely used in the preparation of perovskite solar cells (PSCs) due to its fast carrier transfer and strong light scattering capacity, but the trap states of TiO 2 will cause serious recombination, which will affect the performance of the PSCs to a great extent. Surface treatment is an efficient way to passivate the surface defects of TiO 2 and improve the performance of devices. In this study, we introduce a TiO 2 composite treatment strategy that treats TiO 2 films with low-concentration hydrochloric (HCl) solution and ultraviolet ozone (UVO). The composite treatment has no adverse impact on the structure of TiO 2 , which can be confirmed by X-ray diffraction (XRD), atomic force microscope (AFM), and scanning electron microscope (SEM) measurement. X-ray photoelectron spectroscopy (XPS) shows a 4.62% decrease of surface oxygen vacancies in TiO 2 films, which means the composite treatment can reduce the surface defects resulting from oxygen vacancies. To further explore the carrier transfer performance of treated TiO 2 films, the time-resolved photoluminescence (TRPL) spectroscopy and space charge-limited currents (SCLC) measurement were used. The measurements show a decrease to 59.03 ns of average charge carrier lifetime and a 0.023 V reduction of limit voltage, indicating a stronger electron extraction capability and less defect states. Finally, we achieve a power conversion efficiency (PCE) of 18.9% compared to 17.6% for the control PSCs.
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Food Analytical Methods
·
2023
·
IF 2.9
Qiao Fanglin,Chen Yin,Fei Yunjie,Jiang Yanjun,Xiong Xiaohui,Liu Yuanjian
Due to the complex characteristics of food matrix, false positives may occur when using traditional methods to detect aflatoxin B1 (AFB1). In this paper, we utilize the advantages of closed bipolar electrode (BPE) that can effectively avoid the impact of complex food matrices on signal collection, and design a biosensor based on immune competition to detect highly toxic AFB1. The biosensor uses cathode of closed BPE as a functional sensing interface and anode as a signal collection interface. On the functional sensing interface, AFB1 connects to the monoclonal antibody at the top of DNA tetrahedron structure. Under the action of horseradish peroxidase (HRP), 4-chloro-1-naphthol (4-CN) is catalyzed by hydrogen peroxide (H 2 O 2 ) to generate precipitations (4-CD). AFB1 competes with HRP-AFB1 to bind monoclonal antibody, resulting in a decrease in the content of HRP involved in catalytic oxidation reactions and a synchronous reduction in precipitations produced, causing an increase in the intensity of [Ru(bpy) 3 ] 2+ /TPA system electrochemiluminescence (ECL) on the BPE anode. Analysis shows that the relative deviation between the detection results and ELISA is between −4.5 and 9.8%, and there is no significant difference in the performance of the two in detecting AFB1. The BPE-ECL sensor exhibits high sensitivity and specificity in detecting AFB1, with a linear range of 0.01–40 ng mL −1 and a detection limit of 3 pg mL −1 . In the future, it can be applied to the detection of other toxins, with broad application prospects.
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IRANIAN POLYMER JOURNAL
·
2023
·
IF 3.1
Wan Songhan,Chen Songbo,Yang Qingshan,He Xianru
The migration of commercial rubber antioxidants causes significant environmental pollution; thus, environmentally friendly antioxidants are receiving increasing attention in rubber production. Herein, we prepared a green anti-aging composite filler called ZPa via an in situ intercalation polymerization procedure of polydopamine (PDA) on zirconium phosphate ( α -ZrP) for realizing the enhancement of mechanical performance and aging resistance in nitrile butadiene rubber (NBR). Different from commercial antioxidants, the biocompatibility of α -ZrP and PDA made ZPa non-toxic and harmless to the environment. Results showed that PDA increased the interlayer spacing of α -ZrP and promoted its dispersion in matrix. The best reinforcement effect of the composite filler on NBR can be achieved with 5 phr of ZPa. The tensile strength of ZPa/NBR increased by 51% compared to the neat NBR. The outstanding free radical capture capability and the barrier function of ZPa greatly enhanced the ability of NBR to resist aging. The mechanical properties after aging of ZPa-5/NBR were 64% and 21% higher than those of the neat NBR in tensile strength and elongation-at-break values, respectively. The present work also exhibited that ZPa/NBR showed powerful thermal oxidative stability. In brief, ZPa enabled NBR highly competitive for mechanical performance and anti-aging property, which is expected to be utilized in the field of “green” rubber. Graphical Abstract
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