JOURNAL OF MEMBRANE SCIENCE
·
2018
·
IF 6.578
Weicheng Fu,Xueye Wang,Junjian Zheng,Mingxian Liu,Zhiwei Wang
Membrane fouling remains as a critical issue limiting the widespread applications of membrane-based technologies for water and wastewater treatment . In this work, an electrochemical ceramic membrane reactor (ECMR), in which ceramic membrane with built-in Ti mesh acted as cathode/filter and Ti/SnO 2 -Sb-Ce as anodes, was developed for mitigating membrane fouling during wastewater treatment. Linear sweep voltammetry (LSV) and electrochemical impedance spectroscopy (EIS) of Ti/SnO 2 -Sb-Ce electrode demonstrated favorable electrochemical properties . Filtration tests with influent wastewater containing 50 mg/L sodium alginate (SA) showed that TMP increase rate for the ECMR was decreased by 23.9–72.7% compared to the control, dependent on the exertion modes of electric field in this reactor. It was found that all the oxidants contributed to the enhanced antifouling performance of the ECMR due to in-situ membrane cleaning effects while the hydrogen peroxide and active chlorine (Cl T ) played a more important role compared to superoxide anions and hydroxyl radicals. SA was oxidized to a smaller size after reacting with reactive oxygen species (ROS) and Cl T , benefiting the mitigation of membrane fouling in ECMR.
✓ 已验证
产品关联
DOI
高影响因子
MOLECULAR IMAGING AND BIOLOGY
·
2019
·Vol. 22
·期 2
·265-273
·
IF 3.341
Zhao Chao,Liu Chunyi,Tang Jie,Xu Yingjiao,Xie Minhao,Chen Zhengping
Purpose The aim of this study was to optimize the radiolabeling method of [ 18 F]fluoropropyl-(+)-dihydrotetrabenazine ([ 18 F]FP-(+)-DTBZ) to fulfill the demand of preclinical and clinical application. Procedures Optimized labeling conditions were performed by altering the molar ratio of precursor to base (P/B), base species, solvents, reaction temperature, reaction time, and precursor concentration through manual radiosynthesis of [ 18 F]FP-(+)-DTBZ. The conditions with the highest radiochemical yield (RCY) were applied to automated radiosynthesis, and the crude product was purified with a Sep-Pak Plus C18 cartridge. Quality control and stability of [ 18 F]FP-(+)-DTBZ were carried out by HPLC. In vitro cellular uptake and blocking assays were conducted in human neuroblastoma cell line SH-SY5Y. In vivo imaging with small animal positron emission tomography (microPET) was performed with Sprague–Dawley rats. Results Under the optimized conditions (P/K 2 CO 3 = 1:8, heating at 120 °C for 3 min in dimethyl sulfoxide), an RCY of 88.7 % was obtained with 1.0 mg precursor. The optimized reaction conditions were successfully applied to an automated module and gave a high activity yield (AY) of 30–55 % in about 40 min with a > 99.0 % radiochemical purity (RCP) and a > 44.4 GBq/μmol molar activity ( A m ). Stability test displayed that the RCP retained > 98.0 % in 8 h in saline and in phosphate buffer saline (PBS, pH 7.4). In vitro cellular uptake assay showed accumulation of [ 18 F]FP-(+)-DTBZ in SH-SY5Y cells, which could be significantly inhibited by vesicular monoamine transporter 2 (VMAT2) inhibitor DTBZ. MicroPET images of rat brain displayed that the striatum showed the highest uptake with a standardized uptake value (SUV) of 3.91 ± 0.30 at ~ 70 min. Co-injection with DTBZ (1.0 mg/kg) resulted in a 75 % decrease of the striatal SUV, confirming the specificity of [ 18 F]FP-(+)-DTBZ to VMAT2. Conclusions We obtained an optimized radiolabeling method of [ 18 F]FP-(+)-DTBZ and successfully applied it to a commercial available module. The automated synthesis gave a high AY and RCP of [ 18 F]FP-(+)-DTBZ with high and specific binding to VMAT2, facilitating its routine application for VMAT2 tracing.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLoS One
·
2019
·Vol. 14
·期 4
·e0215084
·
IF 2.776
Xiaoqin Wang,Zhi-Min Li,Qingyue Li,Mingsong Shi,Lingling Bao,Dingguo Xu,Zhimin Li
Fermentation-respiration switch protein (FrsA) was thought to play an important role in controlling the metabolic flux between respiration and fermentation pathways, whereas the biochemical function of FrsA was unclear yet. A gene coding for FrsA protein from Vibrio vulnificus was chemically synthesized. The recombinant VvFrsA was expressed as a soluble protein and purified by Ni-NTA affinity chromatography. The protein had a subunit molecular weight of ca . 45 kDa by SDS-PAGE and preferred short-chain esters when p-nitrophenyl alkanoate esters were used as substrates. Optimum condition for VvFrsA was found to be at pH 9.0 and 50 °C. The protein retained high esterase activity at alkaline condition and would denature slowly at over 50 °C. With p-nitrophenyl acetate as the substrate, the K m and k cat were determined to be 18.6 mM and 0.67 s -1 , respectively, by steady-state kinetic assay. Molecular dynamics simulation and docking model structure revealed that p-nitrophenyl acetate could be the substrate of VvFrsA. In conclusion our results demonstrated that the protein was able to catalyze the hydrolysis of esters, especially p-nitrophenyl acetate, for the first time.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Smart Materials and Structures
·
2019
·Vol. 28
·期 10
·105006
·
IF 3.543
Guangming Tian,Guangming Zhu,Shuogui Xu,Tianning Ren
Plasmonic materials possess good optical properties in effectively converting solar energy into heat, here a series of sunlight responsive shape memory composites films based on acrylate-terminated Poly (ε-caprolactone) (PCL) and titanium nitride nanoparticles (TiN NPs) are prepared via base-catalyzed Michael addition. Compared with conventional free radical polymerization or physical blending, this paper constructs a network architecture from view of structural controllability, favoring a subsequent flexible and elastic materials as well as fine dispersion of TiN NPs in the cross-linked networks, the resultant materials show an outstanding mechanical and the incorporated TiN NPs enable rapid temperature increase over the melting temperature of PCL and trigger the shape change of the composite with good shape recovery rate. The effect of light density and TiN NPs concentration on composite film are elucidated and investigated systematically, it is found that the amount of TiN NPs content virtually has a negligible effect on the surface temperature of the films when subjected to a specific illumination, however, light intensity has a remarkable influence on it. Furthermore, an electronics device switch actuated by 160 mw cm−2 simulated sunlight irradiation is demonstrated and envisioned for flexible electronic applications.
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2019
·
IF 5.714
Xiaoxin Han,Xueqiang Lu,Rolf D. Vogt
Microplastic pollution in the environment has received growing attention worldwide. A major impediment for accurate measurements of microplastics in environmental matrixes is to extract the particles. The most commonly-used method for separation from soil or sediment is flotation in dense liquid based on the relatively low density of plastic particles. This study provides an improved and optimized process for extraction of microplastic particles by modifying the floatation technique and floatation solution. Microplastics in soils and sediments are extracted by adding 200 g dry soil or sediment sample to 1.3 L mix of the saturated NaCl and NaI solutions in a volume ratio of 1:1 and aerating for 40 s then filtering the supernatant. The accuracy and precision of the new approach is validated by recovery experiments using soil and sediment samples spiked with six common microplastic compounds: polyethylene (PE), polyethylene terephthalate (PET), polypropylene (PP), polyvinyl chloride (PVC), polystyrene (PS) and expanded polystyrene (EPS), and comparison with the previous method. The optimized approach is further compared with the previous approach using the real soil and sediment samples.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COMBUSTION AND FLAME
·
2018
·
IF 4.494
Xiaoyuan Zhang,Lili Ye,Yuyang Li,Yan Zhang,Chuangchuang Cao,Jiuzhong Yang,Zhongyue Zhou,Zhen Huang,Fei Qi
Acetaldehyde oxidation was investigated in a jet-stirred reactor at temperatures from 460 to 900 K, equivalence ratios from 0.5 to 4.0 and pressures of 710–720 Torr. Reactive intermediates under low-temperature conditions, such as methylperoxy, methylhydroperoxide and ketohydroperoxide were detected using synchrotron vacuum ultraviolet photoionization mass spectrometry (SVUV-PIMS). A kinetic model for acetaldehyde oxidation was constructed by incorporating recent theoretical and modeling progress of acetaldehyde kinetics, as well as the calculated results of H-atom abstraction reactions of acetaldehyde by acetylperoxy, methylperoxy and methoxy in this work. The present model was then comprehensively validated against the measurements in this work and the experimental data from literature. Modeling analysis reveals that the main chain-branching pathways of acetaldehyde under low-temperature oxidation conditions are the decomposition of acetylhydroperoxide and methylhydroperoxide. The second O 2 -addition process was verified to exist in the low-temperature oxidation of acetaldehyde as ketohydroperoxide was observed, while its contribution to the overall reactivity was found to be minor under present investigated conditions. On the other hand, the sub-mechanism of acetylperoxy is crucial in the low-temperature regime while the methyl oxidation mechanism dominates in the negative temperature coefficient (NTC) region at all equivalence ratios investigated in this work.
✓ 已验证
产品关联
DOI
高影响因子
Journal of Cleaner Production
·
2018
·
IF 5.651
Li Cui,Jiugang Yuan,Ping Wang,Xingping Wang,Xuerong Fan,Qiang Wang
Phosphorylated wool with enhanced ability to absorb cationic dyes is helpful to dye wool fabrics using cationic dyes with good dyeing properties, which is more economic and ecofriendly compared to the traditional dyeing process of acid dyes with poor wet fastness. Phosphorylation can be performed by applying chemical reagents which tend to cause a problem of environmental pollution and the fibers can be damaged seriously under harsh reaction conditions, or using enzymes which are currently somewhat discouraged due to significant cost of the enzymes and their decreased stability at the reaction conditions. In this study, non-toxic glucose 6-phosphate was applied to phosphorylated modification of wool via Maillard reaction under a mild condition to enhance the dyeing properties of wool fabric for cationic dyes. The reaction between glucose 6-phosphate and wool keratin was demonstrated from the results of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), particle diameter, Fourier transform infrared spectra (FTIR) and Energy Dispersive X-ray test (EDS). It was found that phosphorylation modification scarcely changed the mechanical properties of the wool fabric. Maillard browning on the wool could be avoided by accurately controlling the reaction time. Dyeing properties of phosphorylated wool fabric with cationic dyes were markedly enhanced. The exhaustion of phosphorylated wool fabric for two cationic dyes: Cationic Brilliant Red 5 GN and Brilliant Yellow B 6 GL increased from 38.6% to 18.9%–84.5% and 67% respectively by comparing with that of the wool fabric without modification and better color strength (K/S) and very good wash fastness properties obtained. The results indicated that phosphorylated modification of wool with glucose 6-phosphate could be a potential, safe and cleaner approach to develop a more efficient and economic dyeing process for wool fibers with cationic dyes.
✓ 已验证
DOI
高影响因子
International Journal of Environmental Research and Public Health
·
2018
·Vol. 15
·期 2
·239
·
IF 2.145
Lin Ma,Chunhai Jiang,Zhenyu Lin,Zhimin Zou
Biosorption using agricultural wastes has been proven as a low cost and efficient way for wastewater treatment. Herein, grape peel treated by microwave- and conventional-hydrothermal processes was used as low cost biosorbent to remove methylene blue (MB) from aqueous solutions. The adsorption parameters including the initial pH value, dosage of biosorbents, contact time, and initial MB concentration were investigated to find the optimum adsorption conditions. The biosorbent obtained by microwave-hydrothermal treatment only for 3 min at 180 °C (microwave-hydrothermal treated grape peel, MGP) showed faster kinetics and higher adsorption capability than that produced by a conventional-hydrothermal process (hydrothermal treated grape peel, HGP) with a duration time of 16 h. The maximum adsorption capability of MGP under the optimum conditions (pH = 11, a dosage of 2.50 g/L) as determined with the Langmuir model reached 215.7 mg/g, which was among the best values achieved so far on biosorbents. These results demonstrated that the grape peel treated by a quick microwave-hydrothermal process can be a very promising low cost and efficient biosorbent for organic dye removal from aqueous solutions.Keywords:grape peel;biosorbent;biosorption;methylene blue;wastewater treatment
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 6
·4324-4330
·
IF 3.201
Zhonghua Xue,Lulu Xiong,Hao Peng,Honghong Rao,Xiuhui Liu,Xiaoquan Lu
Rapid determination of cysteine in aqueous solution is important for the diagnosis and treatment of some diseases. We herein present a simple yet facile colorimetric indicator-displacement assay for cysteine in aqueous solution with a sensitive optical readout based on the competitive binding affinity between the 1-(2-pyridylazo)-2-naphthol (PAN) indicator and cysteine target towards the host Zn2+. In the absence of cysteine, Zn2+ can induce an appreciable yellow-to-pink color change for the PAN solution through the formation of a PAN–Zn2+ complex. However, adding cysteine to the above PAN–Zn2+ complex solution resulted in a ligand exchange reaction between PAN and cysteine with about 57 nm blue-shift in the absorbance maximum (from 527 nm to 470 nm), accompanied by a visible color change of the solution from pink to yellow. The obtained indicator-displacement assay provides a good detecting performance for cysteine with a detection limit of 7.32 μM (S/N = 3) using a UV/vis spectrometer as well as with a good linear range from 25 to 1000 μM. In particular, the proposed sensing system could be successfully used for the fabrication of a colorimetric INHIBIT logic gate and converted into the test paper and image capture analytical devices for the onsite visual determination of cysteine in practical applications.
✓ 已验证
产品关联
DOI
高影响因子
Micromachines
·
2018
·Vol. 9
·期 6
·274
·
IF 2.222
Zihao Li,Chenggang Zhu,Zhihe Guo,Bowen Wang,Xiang Wu,Yiyan Fei
The detection of small molecules has increasingly attracted the attention of researchers because of its important physiological function. In this manuscript, we propose a novel optical sensor which uses an optofluidic microbubble resonator (OFMBR) for the highly sensitive detection of small molecules. This paper demonstrates the binding of the small molecule biotin to surface-immobilized streptavidin with a detection limit reduced to 0.41 pM. Furthermore, binding specificity of four additional small molecules to surface-immobilized streptavidin is shown. A label-free OFMBR-based optical sensor has great potential in small molecule detection and drug screening because of its high sensitivity, low detection limit, and minimal sample consumption.Keywords:label-free sensor;optofluidic microbubble resonator;detection of small molecules
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2018
·
IF 6.578
Hui Wang,Wenjia Hou,Fu Liu,Qiu Han,Tiantian Li,Haibo Lin,Gang Deng,Jidong He
It is challenging to improve the hemocompatibility of commercial dialyzers packaging more than 10,000 fibers. Herein, we report the preparation and evaluation of a self-anticlotting Polysulfone dialyzer via an interface crosslinking approach. By modulating the conformation of sulfate, carboxyl groups and pyrrolidone (VP) in heparin-like polymers, the hemocompatibility of the dialyzer was significantly improved. It was found that the sequential structure of functional groups influenced the anticoagulant performances substantially. The hollow fiber modified by the blended polymer (Poly (N-vinyl-2-pyrrolidone- co -triethoxyvinylsilane) and poly (Sodium 4-vinylbenzenssulfonate- co -triethoxyvinylsilane- co -Acrylic acid) (P(VP-VTES)- b -P(SSNa-VTES-AA))) exhibited better hemocompatibility than the contrast membrane modified by poly (Acrylic acid- co -triethoxyvinylsilane- co -N-vinyl-2-pyrrolidone- co - - co -Sodium 4-vinylbenzenssulfonate) (P(AA-VTES-VP-SSNa)). The chemical composition was determined by X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectra (FTIR). The morphology of the hollow fiber membranes was observed by a scanning electron microscopy (SEM). The thrombin time (TT), plasma fibrinogen (FIB), activated partial thromboplastin time (APTT), prothrombin time (PT) and protein adsorption were measured to examine the hemocompatibility. The modified hollow fiber showed self-anticlotting properties with excellent protein resistance, remarkably prolonged coagulation time (APTT > 600 s), suppressed complement activation and nontoxicity to osteoblasts. The modified dialyzer showed practicable ultrafiltration coefficient and clearance performance, indicating its great potential application in dialysis treatment.
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 18
·9762-9769
·
IF 2.936
Chen Li,Yun-shan Wei,Peng Wen,Kun Feng,Min-hua Zong,Hong Wu
A novel electrospun colon-specific delivery system for salmon calcitonin (SCT) was developed to improve its stability and bioavailability. Firstly, the pectin-coated SCT liposomes were prepared by film dispersion method and then a liposomes/sodium alginate/polyvinyl alcohol fiber mat was fabricated by electrospining. Scanning electron microscopy analysis indicated that the obtained nanofibers were uniform and smooth with an average diameter of about 350 nm. The release of SCT in different simulated digestive fluids was studied and corresponding release kinetics models were built. It was found that the fiber mat containing pectin-coated SCT liposomes had better stability and colon-specific properties compared with that containing uncoated SCT liposomes and the release of SCT in the colon followed the case II transport mechanism. In addition, there is no significant change in the bioactivity of released SCT measured by ELISA. This study shows that the electrospun colon-specific fiber mat is a potential delivery system for bioactive peptides.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）