BIOSENSORS & BIOELECTRONICS
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2016
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IF 7.476
Wei Wang,Ting Bao,Xi Zeng,Huayu Xiong,Wei Wen,Xiuhua Zhang,Shengfu Wang
In this work, a novel and ultrasensitive electrochemical biosensor was constructed for DNA detection based on functionalized gold clusters/graphene nanohybrids (AuNCs/GR nanobybrids) and exonuclease III (Exo III)-aided cascade target recycling. By utilizing the capacity of GR as universal template, different metal nanoclusters including AuNCs/GR nanobybrids and PtNCs/GR nanohybrids were synthesized through convenient ultrasonic method. Exo III-aided cascade recycling was initiated by target DNA, generating the final cleavage product (S2), which acted as a linkage between capture probe and the functionalized metal nanoclusters/GR conjugates in the construction of the biosensor. The AuNCs/GR-DNA-enzyme conjugates acted as interfaces of enzyme-catalyzed silver deposition reaction, achieving DNA detection ranging from 0.02 fM to 20 pM with a detection limit of 0.057 fM. In addition, PtNCs/GR-DNA conjugates presented peroxidase-like activity and the functionalized PtNCs/GR nanohybrids-based electrochemical biosensor also realized DNA detection by catalyzing the 3,3′,5,5′-tetramethylbenzidine-hydrogen peroxide (TMB-H 2 O 2 ) system to produce electrochemical signal. This metal clusters/GR-based multiple-amplified electrochemical biosensor provided an universal method for DNA detection.
研究领域:
生物化学
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纳米技术
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分析化学
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF ENVIRONMENTAL ENGINEERING
·
2020
·Vol. 146
·期 4
·04020019
·
IF 1.264
Junru Bu,Xinkai Zhou,Huan Liu,Chunmian Lin
The roles of H2O2 in supercritical water oxidation (SCWO) were investigated. The results showed that some of the H2O2 could persist for a short time in SCW, and organic pollutants were mainly oxidized by the O2 generated from the H2O2 in SCWO. However, the introduction of Fe2+ into the SCWO system with H2O2 formed a new Fenton oxidation environment, i.e., supercritical Fenton oxidation (SCFO), which showed the cooperative effect of Fenton oxidation and SCWO. Comparative experiments of phenol and p-aminophenol (PAP) in SCWO and SCFO, respectively, were carried out. The results showed that, in the SCFO system, the CO2 yield from the oxidative degradation of phenol-simulated wastewater (766.0 mg/L) in 45s could reach 73.3%, much higher than that in the SCWO (49.2%) under the same conditions. After 8 min, the CO2 yield in SCFO (97.4%) was also significantly higher than that in SCWO (89.4%). The degradation efficiency of PAP-simulated wastewater (272.5 mg/L) in the SCFO system exceeded 94.3%, especially under acidic conditions, and the degradation efficiency reached 97.4%, much higher than that in the SCWO system (86.2%).
研究领域:
环境工程
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化学工程
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水处理技术
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高级氧化工艺
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污染控制
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DOI
高影响因子
ACS Nano
·
2019
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IF 13.903
Peiru Wang,Weitao Yang,Shuzhan Shen,Chao Wu,Long Wen,Qian Cheng,Bingbo Zhang,Xiuli Wang
Elevated hydrogen peroxide (H2O2) in biological tissues is generally recognized to be relevant to the carcinogenesis process that regulates the proliferative activity of cancer cells and the transformation of malignant features. Inspired by this observation, it can be hypothesized that imaging H2O2 in the tumor microenvironment (TME) could help diagnose tumor types and malignancy, and even guide precise therapy. Thus, in this study, a noninvasive photomedicine strategy is demonstrated that leverages the different levels of H2O2 in the TME, and two representative skin cancers, malignant melanoma (MM, clinically higher incidence of metastasis and recurrence) and cutaneous squamous cell carcinoma (cSCC, relatively less dangerous), are differentially diagnosed. The working probe used here is one we previously developed, namely, intelligent H2O2 responsive ABTS-loaded HRP@Gd nanoprobes (iHRANPs). In this study, iHRANPs have advantages over ratiometric imaging due to their bimodal imaging elements, in which the inherent magnetic resonance imaging (MR) mode can be used as the internal imaging reference and the H2O2 responsive photoacoustic (PA) imaging modality can be used for differential diagnosis. Results showed that after intravenous injection of iHRANPs, the tumor signals on both MM and cSCC are obviously enhanced without significant difference under the MR modality. However, under the PA modality, MM and cSCC can be easily distinguished with obvious variations in signal enhancement. Particularly, guided by PA imaging, photothermal therapy (PTT) can be precisely applied on MM, and a strong antitumor effect was achieved owing to the excessive H2O2 in the TME of MM. Furthermore, exogenous H2O2 was injected into cSCC to remedy H2O2 deficiency in the TME of cSCC, and an evident therapeutic efficacy on cSCC can also be realized. This study demonstrated that MM can be differentially diagnosed from cSCC by noninvasive imaging of H2O2 in the TME with iHRANPs; meanwhile, it further enabled imaging-guided precision PTT ablation, even for those unsatisfactory tumor types (cSCC) through exogenously delivering H2O2.
研究领域:
肿瘤学
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光医学
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生物医学工程
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分子影像
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诊疗一体化
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产品关联
DOI
PubMed
高影响因子
Applied Materials Today
·
2021
·
IF 10.041
Xu Chen,Xufeng Zhu,Youcong Gong,Guanglong Yuan,Jieqiong Cen,Qiaoshan Lie,Yida Hou,Gang Ye,Shengming Liu,Jie Liu
Excessive reactive oxygen species (ROS) is the main factor leading to high mortality in local inflammation and sepsis. Therefore, developing efficient ROS scavenger in pro-inflammatory immune cells is an urgent strategy for therapy local inflammatory and sepsis injury. Herein, we constructed a novel mesoporous selenium-hyaluronic acid nanoenzyme therapeutic system (MSe-HA NPs) for therapy local inflammatory and sepsis injury by targeting eliminate ROS in inflammatory macrophage. In detail manners, the high specific surface area of mesoporous selenium nanoenzyme exhibited ultra-high activity like glutathione peroxidase (GPx), which catalytic speed of H 2 O 2 scavenging was increasing to 2.02 times compare with solid Se NPs. Moreover, the broad spectrum of ROS eliminating capacity further promoted ability of MSe NPs in eliminating various ROS. More interestingly, we found that choose HA as the modification not only endowed the MSe NPs targeting to inflammatory macrophage, but the ROS degradable property of HA could enhance the MSe NPs to eliminating ROS. In vivo , MSe-HA NPs after injection showed that simultaneously cured the local inflammation and sepsis induced with LPS by quickly eliminating excessive ROS. Especially in sepsis mouse models, the survival rate and the organic dysfunction significantly improved after treated with MSe-HA NPs. Thus, this novel mesoporous nanozyme provides an effective strategy for the design of nanomedicine and the treatment of inflammation-related diseases.
研究领域:
纳米技术
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药学
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免疫学
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炎症研究
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败血症治疗
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2014
·
IF 6.451
Pengjuan Ni,Haichao Dai,Yilin Wang,Yujing Sun,Yan Shi,Jingting Hu,Zhuang Li
In this study, a facile method to sensitively detect melamine and highly improve the peroxidase-like activity of bare gold nanoparticles (Au NPs) at the same time is proposed for the first time. It is interesting to find that the addition of melamine could improve the peroxidase-like activity of Au NPs. By coupling with 3,3′,5,5′-tetramethlybenzidine (TMB)–H 2 O 2 chormogenic reaction, a novel method for colorimetic detection of melamine is developed. The detection limit of this method is as low as 0.2 nM with the help of UV–vis spectroscopy and 0.5 µM by naked-eye observation, both which are far below the US food and Drug Administration estimated melamine safety limit of 20 µM. In addition, the present method is successfully applied for the detection of melamine in raw milk and milk powder. More importantly, the proposed method could also improve the peroxidase-like activity of Au NPs, which may not only provide a new approach to develop effective nanomaterials-based mimetic enzyme , but also irradiative to develop new applications for Au NPs in varieties of cost-effective and simple sensors in medicine, biotechnology and environmental chemistry.
研究领域:
分析化学
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纳米技术
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食品科学
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环境化学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2020
·Vol. 10
·期 11
·6647-6653
·
IF 3.119
Huali Jin,Di Zhang,Yong Liu,Min Wei
An electrochemical aptasensor is fabricated for lead ion (Pb2+) detection based on catalytic hairpin assembly as signal amplification. Biomass porous carbon derived from soybean straw (PCs) is used to load platinum nanoparticles (PtNPs), which are introduced as mimetic enzymes to catalyze the hydroquinone–H2O2 system. In the presence of Pb2+, the complementary DNA (cDNA) releases from aptamer-cDNA and hybridizes with hairpin DNA1 (HP1) on the electrode surface. After bio-hairpin DNA2 (bio-HP2) is added, hybridization between HP1 and bio-HP2 further releases cDNA, which participates in the next cycle and triggers amplification, eventually forming a large number of bio-HP2/HP1 on the electrode surface. Then PtNPs@PCs immobilize on the electrode surface by specific binding of streptavidin with biotin, and catalyze the oxidation of hydroquinone in the presence of H2O2. The produced electrochemical signal depends on the concentration of Pb2+. The developed biosensor exhibits a wide linear range from 50 pM to 1000 nM with a detection limit of 18 pM, and high selectivity for Pb2+ over other environmentally relevant metal ions at concentration ratios of 100. The recoveries of Pb2+ in real samples were 93.5–108%. Thus, the proposed biosensor can provide an efficient method for sensitive and selective detection of Pb2+ in real samples.
研究领域:
分析化学
·
电化学
·
生物传感器
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环境科学
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产品关联
DOI
高影响因子
Nanoscale
·
2024
·Vol. 16
·期 16
·8162-8176
·
IF 5.8
Xinmeng Zhang,Mingyue Bai,Lei Ge,Yuanyuan Yao
The slow redox rate of hydrogen peroxide (H2O2) in neutral environments makes the H2O2 sensor inadequate for the detection of low levels of signalling molecules. The aim of this study is to fabricate a flexible sensing electrode by hydrothermally loading micro-nanometer Ni and Co(OH)2 on carbon cloth (CC) and electrochemically depositing poly(3,4-ethylenedioxythiophene) (PEDOT) on the surface of the electrode. The sensor presented high sensitivity (10.43 mA mM−1 cm−2), a wide detection range (0.033–120.848 mM), a low detection limit (0.92 nM), high stability, and excellent anti-interference performance in neutral solutions. Ni–Co(OH)2 provides abundant active sites while CC solves their agglomeration phenomenon and conductivity. The PEDOT film offers heightened conductivity, hydrophilicity, interfacial stability, and an electrochemically active surface area (ECSA). The side area of the chrysanthemum petal like PEDOT is 39 ± 7 times the bottom area, and PEDOT increases the ECSA of the composite to six times that of CC. Electrochemical precise control of PEDOT morphology to improve sensor performance provides a new strategy for the application of PEDOT in sensors.
研究领域:
材料科学
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生物医学工程
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分析化学
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Jun Cong,Shao-hua Luo,Qi Sun,Xin Liu,Yi-Kai Wang,Sheng-xue Yan,Peng-wei Li
Efficient leaching and recovery of LiFePO 4 (LFP) in spent lithium-ion batteries (LIBs) is a key step to realize the recycling of lithium resources. Herein, H 2 SO 4 -H 2 O 2 is selected as the leaching agent to achieve selective leaching of lithium from spent LFP cathode materials, and the response surface method (RSM) serves to define the optimal leaching process. The experimental findings signify that when the concentration of H 2 SO 4 is 0.315 mol·L -1 , H 2 O 2 /Li molar ratio of 2.13, H 2 SO 4 /Li molar ratio of 0.62 (solid–liquid ratio of 96.87 g·L -1 ), leaching temperature of 62 °C and leaching time of 90 min, the leaching rate of Li + can reach as high as 95.23 %. Thermodynamic analysis, infrared spectroscopy and Mössbauer spectrum are used to clarify the reaction mechanism, that is, Fe 2+ in LiFePO 4 is oxidized to Fe 3+ by H 2 O 2 in situ to form FePO 4 , and Li + is released into the solution. Moreover, chemical precipitation method is used to efficiently recover the lithium resources in the form of lithium carbonate. By applying Box-Behnken design (BBD) and RSM to optimize the recovery technology to reach the global optimal solution to achieve the highest recovery rate of Li + in spent LFP. The experimental results show that under the optimal conditions of a Na 2 CO 3 /Li molar ratio of 0.6, a reaction temperature of 90 °C, and a C (Li + ) of 20.28 g·L -1 , the precipitation rate of Li + reaches 89.22 %. Moreover, the physical properties of the recovered Li 2 CO 3 are analyzed, and the content of Li 2 CO 3 in the recovered product is as high as 99.62 %. Additionally, the economic analysis and environmental impact analysis of the whole process of hydrometallurgical recovery of cathode materials are carried out by EverBatt 2023 model, which confirmed that hydrometallurgy is the extremely satisfactory method for recovering spent LFP cathode materials.
研究领域:
环境科学
·
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Weitong Wang,Yongdong Tang,Xiaozhe Liu,Bei An,Huijie He,Meijing Cao,Rongzhe Zhao,Yaxuan Chen,Xiaojing Yu,Shengchun Yang,Zhimao Yang,Bin Wang
B N pairs create push-pull coupling to upshift p-band center for optimal *OOH adsorption. • B,N co-doped carbon achieves 99% H 2 O 2 selectivity over wide window in 2e − ORR. • The catalyst yields 788 mmol g cat −1 h −1 H 2 O 2 in H-cell and stable 250 mA cm −2 operation for 14 h. • B N synergy breaks π-conjugation uniformity to enable near-unity 2e − ORR. Selective electrocatalytic two-electron oxygen reduction (2e − ORR) offers a green alternative to the energy-intensive anthraquinone process for hydrogen peroxide (H 2 O 2 ) production. However, the inert π-conjugation of carbon catalysts homogenizes the electronic environment of active sites, making it intrinsically difficult to achieve the optimal *OOH adsorption energy essential for high 2e − selectivity. In this study, we developed B,N co-doped carbon catalysts through a cost-effective pyrolysis method, achieving 99% H 2 O 2 selectivity across a remarkably wide potential window (0.20–0.70 V vs. RHE), an H-cell production rate of 788 mmol g cat −1 h −1 , and sustained operation in a flow cell at 250 mA cm −2 for 14 h with a Faradaic efficiency above 80%. It was demonstrated that engineering adjacent B N pairs within a carbon matrix established a localized push-pull electronic coupling that precisely upshifts the p-band center of active carbon atoms into the theoretically predicted “golden zone” for *OOH adsorption via combined experimental and theoretical analysis. These findings highlight the critical role of heteroatom doping in tailoring electronic structures and provide a scalable strategy for efficient, environmentally friendly H 2 O 2 production using carbon-based catalysts. This work develops B,N co-doped carbon catalysts that achieve 99% H 2 O 2 selectivity and 8 mol g cat −1 h −1 yield via 2e − ORR, leveraging synergistic doping and pyrrolic nitrogen optimization for scalable production. Download: Download high-res image (113KB) Download: Download full-size image
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产品关联
DOI
高影响因子
ChemistrySelect
·
2025
·Vol. 10
·期 43
·e04409
·
IF 2
Jialong Yang,Guangqun Zhai
The effect of aerobic dioxygen (O 2 ) residues and hydrogen peroxide (H 2 O 2 ) on dithiobenzoate-mediated reversible addition-fragmentation chain transfer (RAFT) polymerization of methyl methacrylate (MMA) was studied. 2-cyano-2-propyl dithiobenzoate (CPDB) displayed excellent thermally oxidative stability against O 2 in O 2 -rarefied dimethylsulfoxide (DMSO) but was readily oxidized by H 2 O 2 . Azo-initiated CPDB-mediated RAFT polymerization of MMA in DMSO under different media led to multi-component products, consisting of PMMA macro-CTAs, MMA-terminated oligomers and low-MW byproducts, while nitrogen deoxygenating and headspace eliminating gave rise to a comparable CTA efficiency, higher than that headspace limiting. H 2 O 2 -initiated CPDB-mediated headspace-free RAFT polymerization of MMA smoothly proceeded in DMSO even at 50 °C, suggesting the co-initiation by CPDB, and also formed predominant PMMA macro-CTAs, negligible oligomers and some low-MW byproducts. H 2 O 2 -initiated CPDB-mediated headspace-free RAFT polymerization of MMA at 70 °C displayed well-controlled behaviors with 2–5 wt.% byproducts, and the high fidelity of the PMMA macro-CTAs was confirmed by the chain extension via azo-initiated RAFT polymerization and Cu I -catalyzed concurrent ATRP/RAFT polymerization. Graphical AIBN-initiated dithiobenzoate-mediated RAFT polymerization of MMA in O 2 -rarefied DMSO under headspace-free media led to well-controlled behaviors comparable to that under N 2 deoxygenating. H 2 O 2 -initiated RAFT polymerization manifested the unprecedented acceleration by dithiobenzoate; ascribed to its oxidation by H 2 O 2 ; and well-controlled polymerization behaviors.
研究领域:
高分子化学
·
自由基聚合
·
可控/活性聚合
·
有机合成
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Zhewen Shao,Kexin Xiang,Shuchang Liu,Feng Hong,Ye Gao,Enhong Lei,Jinping Jia,Yingping Huang
Presently, fast degradation of organic wastewater has been achieved by the synergetic method of hydrodynamic cavitation and H2O2 at a laboratory scale, while the physico-chemical effect, especially the turbulence characteristics on the degradation process remains lacking. This paper presents investigation on the degradation of dyeing wastewater containing methylene blue (MB) under neutral condition, within a vortex-based hydrodynamic cavitation (VBHC) reactor. Operating conditions are firstly optimized and then used to collaborate with H2O2 to study the synergetic effects of this hybrid treatment method. The turbulence characteristics are subsequently obtained by a computational fluid dynamics (CFD) simulation, and the correlation between them and the degradation process of MB is revealed. The results indicate that the highest degradation rate of 91.95 % is achieved under optimal conditions. Higher inlet pressure (4.5 bar) owns the thickest vapor volume fraction, greatest vapor transfer rate, and largest size of cloud cavity in the vortex core region, indicating the strongest cavitation intensity; meanwhile, moderate solution temperature (30 ℃) also favors for the greatest cavitation intensity inside the reactor. The optimal cavitation intensity activates the considerable production of reactive oxygen species, and results in good consistency with the MB degradation performance, which predominately occurs in the annular region around the vortex rope. This research unveils the rationale behind the physical essences of the VBHC process and its influence on the degradation efficacy of MB, which can provide reliable guidance for further development of cavitational reactors to degrade organic pollutants by the VBHC/H2O2 hybrid treatment method.
研究领域:
环境科学
·
化学工程
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Xin Zhang,Hongye Wu,Mao Chen,Zhili Li
Magnetic iron oxide confined in carbon capsules/biochar composite (FeOx@g-BC) was created using in-situ synchronous pyrolysis of alkali lignin as a low-cost carbon source. Characterization results indicated the FeOx was confined in carbon nanotubes and carbon capsules, inhibiting growing of nanoparticles and deactivation. The composite catalyst demonstrated significant activity in activating H 2 O 2 for the degradation of persistent organic pollutants in water over a wide pH range. Particularly, tetracycline (TC) could be completely degraded within 25 min, even at a high pH of 6.8, which performed much better than previously reported Fenton-like catalysts. Moreover, the excellent magnetism of FeOx@g-BC aided in its recovery and reuse. The stability of FeOx@g-BC recycling was also measured by continuous cycles of reactions. According to ESR analysis and free radical quenching studies, OH and 1 O 2 were discovered as the dominant active species governing the degradation of TC, and two pathways of TC degradation were proposed. This study developed a novel heterogeneous catalyst for catalytic degradation of persistent organic contaminants in water by the value-added usage of lignin.
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）