SENSORS AND ACTUATORS B-CHEMICAL
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2022
·
IF 9.221
Ailing Su,Yuan Liu,Xiumian Cao,Weiqing Xu,Chongyang Liang,Shuping Xu
A new gene detection platform based on surface-enhanced Raman spectroscopy (SERS) and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a system is developed, which is applied for virus gene detections. CRISPR/Cas12a activated by CRISPR ribonucleic acid (crRNA) can recognize and lock the complementary target double-stranded deoxyribonucleic acid (dsDNA), followed by the cis -cleavage on the dsDNA and the trans -cleavage on the nearby linker single-stranded DNA (ssDNA). Target-induced trans -ssDNA cleavage leads to a failure in an aggregation behavior of the designed SERS probe pair that was bridged by the linker ssDNA, which causes the SERS intensity reduced relative to the control trial that gives much stronger SERS due to the severe aggregation state of SERS probes caused by intact linker ssDNA. This sensing method achieves the highly sensitive detections of human papillomavirus (HPV) genes in serum and pseudovirus within 40 min. The detection sensitivity of this CRISPR/Cas-SERS platform can reach pM. Owing to the flexible designs of the DNA probe and crRNA, this CRISPR/Cas-SERS sensing system is a universal gene detection tool, which can be applied for the in-vitro diagnosis field and point-of-care testing.
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高影响因子
VIBRATIONAL SPECTROSCOPY
·
2022
·
IF 2.382
Chang Wang,Qingqiang Cui,Tian Yue,Anxin Jiao,Hui Ma,Mengya Zhang,Linqi Zheng,Shuang Li,Guanhua Li,Ming Chen
As for the ultrasensitive diagnosis of biomolecules via surface-enhanced Raman scattering spectroscopy (SERS), the primary requirement is to effectively enhance the activity of nano-substrates. Herein, we report an interesting strategy for further boosting SERS activity by thermal annealing treatment of as-prepared hybrid substrates. Initially, highly dense and monodisperse plasmonic Au nanoparticles (NPs) were loaded on two-dimensional (2D) graphitic carbon nitride (g-C 3 N 4 ) nanosheets . By adopting uric acids (UAs) as probe biomolecules, the g-C 3 N 4 /Au NPs with appropriate ~2.1% Au compositions exhibit much higher Raman signals than that of the hybrids with other Au contents. Besides the enhancement by routinely controlling hybrid components, the further maximizing SERS activity with a ~2.6-fold improvement than the original one can be obtained by thermal annealing of g-C 3 N 4 /Au NPs at 350 ℃ condition. It enables the thermal modified g-C 3 N 4 /Au NPs to be applied for highly sensitive monitoring of UAs in artificial urine. The corresponding detection limit is achieved at the ultralow level of 10 −11 M, which is nearly two orders of magnitude better than that of the unannealed sample. It should be attributed to the stronger synergistic coupling effect by thermal annealing treatment that gives rise to the higher-efficiency photoinduced electron transfer (PIET) between g-C 3 N 4 supports and plasmonic Au NPs. The present work provides a new opportunity for further promoting the SERS activity of prefabricating nano-substrates, holding great potential for practical monitoring of ultra-trace biomolecules in the near future.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
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IF 4.831
Lanxin Xu,Jiali Ma,Dong Chen,Chenjie Gu,Jun Zhou,Tao Jiang
Strapping plasmonic substrate with a reliable ability to anchor molecules and achieve reproducible result provides trustworthy opportunities for flourishing surface-enhanced Raman scattering (SERS) technique. Herein, a facile controllable in-situ anisotropic growth strategy was exploited to anchor gold nanowires (Au NWs) onto two-dimensional g-C 3 N 4 nanosheets (g-C 3 N 4 /Au NWs), facilitating a sensitive and recyclable SERS sensor for gaseous analytes. Benefiting from the attractive enrichment effect of the brush-like surface formed by numerous small Au NWs as well as their rich nanotips-mediated enhancement capability, the hybrid substrate showed an outstanding performance in SERS-based detection of trace 4-Aminothiophenol (4-ATP) molecules, demonstrating a monitoring limitation down to 10 −8 M even in atmosphere. Satisfyingly, under visible light illumination, the efficient green photocatalytic ability derived from the g-C 3 N 4 supporting matrix rendered reusable capability for the substrate, whose SERS signal was kept at a persistent high level throughout 6 cycles. Attributed to the narrow line width of SERS spectrum, the 4-ATP assay under the interference of 2-naphthalenethiol (2-NAT) was acquired in gas phase and the dependable recovery rates from 85.4 to 93.9% were confirmed as well. Thanks to the intriguing features including excellent sensitivity and recyclability, the g-C 3 N 4 /Au NWs substrate proposed here will pave the way toward the potential application of SERS technique in multiplexed gaseous detection.
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
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IF 4.831
Lingyi Zhao,Ruiqin Yang,Yi Wei,Yujing Guo,Qian Zhao,Hongwen Zhang,Weiping Cai
Rapid and flexible detection or accurate recognition of trace drugs is of great importance in cracking down on drug crimes, but it remains to be expected. Here, a solid chip is presented for the efficient detection and recognition of trace opioids (typically morphine) in aqueous solutions based on surface-enhanced Raman spectroscopy (SERS). Firstly, a Au-coated Si nano-cone array (Au-SNCA) is designed and fabricated via Si-based organic colloidal template etching and Au deposition. This Au-SNCA shows three-dimensional nanostructure with high densities of nanotips and deep nanogaps as well as high structural consistency, which exhibits strong SERS activity to morphine and outstanding stability. Then, such Au-SNCA is used as solid SERS chip to detect morphine in aqueous solutions. It has been demonstrated that using such solid chip, trace morphine in solutions could be recognized and detected within 1 min, and the detection limit is 10 -5 mg/mL (∼10 ppb), showing rapid and sensitive detection , which is much better than the previous reports. Meanwhile, the Au-SNCA chip also can be utilized to detect trace morphine in tap water and reservoir water, the recoveries range from 90.4% to 102.4%. Such excellent SERS performance of this Au-SNCA chip is attributed to its special structure which enhances not only local electromagnetic field but also molecular adsorption . The experimental results about the effects of immersion time and concentration show that the adsorption behavior of morphine molecules on such Au-SNCA chip can be explained by the pseudo-second-order kinetic model and Freundlich adsorption mode. Moreover, the Au-SNCA chip is also suitable for the identification of morphine homologues and the broad-spectrum detection of various common drugs. This study presents a practical solid chip and a simple approach for the efficient SERS detection and recognition of trace drugs in solutions. This is of significance to on-site detect drugs in forensic science.
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高影响因子
ANALYTICA CHIMICA ACTA
·
2022
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IF 6.911
Huanhuan Li,Wenhui Geng,Suleiman A. Haruna,Md Mehedi Hassan,Quansheng Chen
In this study, a target-responsive release Surface-enhanced Raman scattering (SERS) sensor was developed for sensitive detection of tetracycline (TTC) using aptamer-gated HP-UiO-66-NH 2 nanochannel strategy. The hierarchically porous zirconium-based metal-organic frameworks (HP-UiO-66-NH 2 MOFs) with high surface areas and the conjugated π-electron system were first synthesized and served as the nanocontainers. The methylene blue (MB) and gold nanoparticles (AuNPs) were then loaded in the pores of HP-UiO-66-NH 2 as the signal probes and capping agent, respectively. Thereafter, aptamer was assembled on the surface of HP-UiO-66-NH 2 based on the π-π stacking interaction. In the presence of TTC, the aptamer “molecular gate” was opened, resulting in the “cargo release” of MB and AuNPs. Hence, the amount of TTC could be determined by monitoring the change of SERS intensity of the supernatant. Under the optimal conditions, a good linear correlation between SERS intensity (886 cm −1 ) and TTC concentration was observed with the concentration from 0.01 to 10000 ng/mL, resulting in a relatively low detection limit of 0.01 ng/mL. Moreover, this method showed a promising practical application in spiked real samples (milk and pork) with recoveries of 93.23–108.79%, which confirmed its great potential in antibiotic detection.
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高影响因子
Nanophotonics
·
2022
·Vol. 11
·期 12
·2875-2889
·
IF 7.923
Mengya Zhang,Yue Tian,Anxin Jiao,Hui Ma,Chang Wang,Linqi Zheng,Shuang Li,Ming Chen
Ultrasensitive trace-detection of toxic and carcinogenic polycyclic aromatic hydrocarbons (PAHs) can ceaselessly propel the environmental surveillance in aqueous ecosystems. Due to the intrinsic nonadsorptive feature of PAHs, the promising technique of surface-enhanced Raman scattering (SERS) spectroscopy has been restricted to diverse functional ligands-based surface modifications of nano-substrates. However, it is not suitable for practical ultralow liquid analysis. Herein, we propose an extraordinary strategy to boost liquid-near infrared (NIR)-SERS activity of plasmonic Au/Ag nano-urchins (NUs) by introducing extra 808 nm laser-triggered an additional strong electromagnetic enhancement into routine 785 nm laser-Raman system. The synergistic double laser-excited NIR-SERS of colloidal Au/Ag NUs enables the Raman signals of crystal violet to be significantly enhanced, approaching a maximum of ∼34-fold increase than that of traditional bare 785 nm laser-excitation. More importantly, the improved liquid-NIR-SERS enables the in-situ detection limit of pyrene molecules in lake water to be achieved at ∼10 −9 M, which is already better than many previous SERS results based on the complicated functionalized nano-substrates. The established double laser-boosted NIR-SERS can also be easily extended to the simultaneous trace-detection of three PAHs-contaminated mixtures, supporting well distinguishable capability. Undoubtedly, the present work opens a new versatile and innovative avenue for ultrasensitive NIR-SERS monitoring of nonadsorptive toxic pollutants in wastewater.
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高影响因子
COMPOSITES PART B-ENGINEERING
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2022
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IF 11.322
Xinyue Wang,Huan Cheng,Yuanzhi Min,Xian Li,Lijun You,Jumei Li
In this study, a crystalline mesoporous ZrO 2 interlayer allowing for enrichment capability and photocatalytic activity was introduced to develop a novel magnetic Fe 3 O 4 /m-ZrO 2 /Ag nanocomposite combined with excellent SERS activity and photocatalytic property. The coverage of Ag nanoparticles on Fe 3 O 4 /m-ZrO 2 /Ag was well controlled to tune their SERS sensitivity by varying the feed amount of AgNO 3 . Several characterization methods were utilized to investigate their composition, morphology and crystal structure. The SERS experiments demonstrated Fe 3 O 4 /m-ZrO 2 /Ag nanocomposites possessed markedly SERS sensitivity, exceptional signal reproducibility and excellent storing stability. They could sense R6G and Cr (VI) at the concentrations of 10 −9 and 5 × 10 −8 M, respectively. Beyond that, the Fe 3 O 4 /m-ZrO 2 /Ag nanocomposites effectively accomplished recyclable detecting R6G and Cr (VI) in 5 cycles through facile photocatalytic removal of pollutants under UV irradiation. Altogether, the novel Fe 3 O 4 /m-ZrO 2 /Ag nanocomposites prepared in this study have high SERS sensitivity and good photocatalytic performance, which is of great significance for environmental detection and ecological restoration.
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高影响因子
JOURNAL OF RAMAN SPECTROSCOPY
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2022
·Vol. 53
·期 8
·1371-1379
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IF 2.727
Xueliang Lin,Xianggang Jia,Jin Yong Lin,Ping Hui Wu,Youliang Weng,Shangyuan Feng
Human blood contains a variety of biomolecules that are highly related to the occurrence, progression, and prognosis of diseases, including cancer. The development of rapid and accurate methods for the detection of these biomarkers with extremely low concentration is vital for improving cancer screening. Herein, a label-free surface-enhanced Raman scattering (SERS) technology was employed to detect human serum samples from healthy volunteers ( n = 51) and breast cancer patients ( n = 58) with the aim of exploring the differences in bio-components between them. Moreover, the influence of measurement location during SERS assay was evaluated due to the occurrence of “coffee-ring (CR)” effect associated with this work. Results showed that the SERS spectra obtained from the CR area can better reflect the overall biochemical information of serum samples and exhibited excellent diagnostic sensitivity (94.8%) and specificity (98%) for identifying breast cancer from healthy groups using principal component analysis and linear discriminant analysis (PCA-LDA) algorithm. This work showed a preliminary study of the serum SERS method for breast cancer detection, providing a potential rapid and accurate liquid biopsy strategy for clinical cancer screening.
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
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IF 4.831
Fu Wan,Yu Lei,Changding Wang,Xinyuan Zhang,Honglin He,Lei Jia,Tingting Wang,Weigen Chen
Accurately evaluating the aging state of oil paper insulation in electrical equipment is a key to ensure the safe operation of the power transformer. For achieving highly sensitive in-situ detection of dissolved furfural in transformer oil with good reproducibility, flower-like silver nanoparticles modified with carbon nanotubes (CNTs@Ag-F-AgNPs) was synthesized by a combination of electroless silver plating and redox method. The large specific surface area and strong adsorption capacity of CNTs@Ag promoted the formation of more “hot spots”. CNTs@Ag-F-AgNPs were adsorbed on Si-Au substrate via mercapto groups on the coupling agent 1′4 phenyldimercaptan molecule (BDT). Using rhodamine 6G (R6G) as probe molecule, the enhanced factor reached 6.96 × 10 9 . Then, the substrate was used for in-situ SERS detection of transformer oil-dissolved furfural at different concentrations and the detection limit was 2.25 mg/L at 1703 cm −1 (Stretching vibration of C = O in furfural molecule), fulfilling requirements of furfural content detection after severe aging of transformer (4 mg/L). Besides, the relative standard deviation (RSD) of characteristic peak intensity at ten different positions was only 1.74%. These results exhibite that three-dimensional nanostructure with high sensitivity and good reproducibility exhibited a wide application range for in situ detection of dissolved trace furfural in transformer oil.
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高影响因子
Nanomaterials
·
2022
·Vol. 12
·期 3
·421
·
IF 5.719
Rongyuan Cai,Lijun Yin,Qian Huang,Ruiyun You,Shangyuan Feng,Yudong Lu
In this work, we developed a new type of SERS probe, which was composed of glass-SiO2[email protected]@Ag nanoparticles (NPs) three-dimensional Surface-enhanced Raman spectroscopy (SERS) substrate. When the laser passed through the quartz glass sheet, on the one hand, the SiO2NPs supporting the[email protected]@Ag NPs increase the roughness of the substrate surface, resulting in a large number of hot spots among nanoparticles. On the other hand, based on the focusing effect of silicon dioxide nanospheres, the laser can better focus on the surface of nanoparticles in the inverted SERS probe, thus showing better SERS enhancement. Furthermore, the[email protected]@Ag NPs core-shell structure was used with 4-mercaptobenzoonitrile (MBN) as an internal standard molecule, and the quantitative determination of tyrosine and urea was realized by internal standard correction method. The standard working curves of the two had good linear correlation with R2above 0.9555. The detection limits of tyrosine and urea were in the range of 2.85 × 10−10M~7.54 × 10−6M, which confirms that this design can be used for quantitative and specific detection of biological molecules, demonstrating great practical significance for the research of diseases such as skin lesions and endocrine disorders.Keywords:SERS;endoscope;internal standard;tyrosine;urea;sweat
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Qinzhi Wang,Yijian Zhao,Tong Bu,Xin Wang,Zhihao Xu,Hui Zhangsun,Li Wang
Surface-enhanced Raman scattering (SERS) with its outstanding performance in ultra-sensitivity has been applied in contaminants monitoring. Herein, a facile strategy of integrating oriented layered MOF nanoarray with controlled electrodeposition of gold nanoparticles via semi-sacrificial template method was proposed to fabricate a versatile self-supporting MOF chip ( [email protected] ) for efficient SERS detection of food contaminants. The S-MOF was ingeniously formed by directly using flexible Ni sheet as both metal source and scaffold, which in favour of greatly improving intrinsic electrical conductivity during electrodeposition process as well as endowing prepared chip with uniform distribution of gold nanoparticles and high stability. Furthermore, amounts of SERS “hot spots” in the oriented layered [email protected] can yield strong electromagnetic field for Raman amplification with superior reproducibility. By virtue of good flexibility and pre-concentration effect of [email protected] , tartrazine (TA), chloramphenicol (CP), imidacloprid (IDP) and crystal violet (CV) in food samples were sensitively detected using three collected approaches (drip coating, soaking and wiping) with low limits of detection (1.0 × 10 −9 M for TA, 8.0 × 10 −11 M for CP, 6.4 × 10 −11 M for IDP and 5.0 × 10 −12 M for CV), wide linear detection ranges (1.0 × 10 −8 – 1.0 × 10 −3 M for TA, 1.0 × 10 −10 – 1.0 × 10 −6 M for CP, 1.0 × 10 −10 – 1.0 × 10 −7 M for IDP and 1.0 × 10 −11 – 1.0 × 10 −7 M for CV) and satisfactory recoveries (96.2 – 125.1%). This method provides a novel strategy to develop an advanced SERS platform for efficient food contaminants monitoring.
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高影响因子
MOLECULES
·
2021
·Vol. 26
·期 21
·6522
·
IF 4.412
Shen Zhang,Zhihui Jiang,Yijin Liang,Yili Shen,Hongmin Mao,Huijuan Sun,Xin Zhao,Xiaoping Li,Wusheng Hu,Guoding Xu,Zhaoliang Cao
Surface-enhanced Raman scattering (SERS) has been widely reported to improve the sensitivity of Raman spectra. Ordinarily, the laser is focused on the sample to measure the Raman spectrum. The size of the focused light spot is comparable with that of micro-nano structures, and the number of micro-nano structures contained in the light spot area (defined as duty cycle) will severely affect the spectrum intensity. In this study, flower-like silver nanostructures were fabricated with a soft lyotropic liquid crystal template in order to investigate the effect of duty cycle. They were observed under a scanning electron microscope, and their spectrum enhancement factor was computed with the obtained Raman spectrum. Then, their duty cycles were measured using a SERS substrate at different locations. A formula was derived to represent the relation between the duty cycle of the nanoflowers and the Raman spectral intensity. This work could promote the actual applications of SERS in high-sensitivity spectrum testing.Keywords:surface-enhanced Raman scattering;lyotropic liquid crystal;duty cycle;silver nanoflower
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高影响因子
危险品化学品经营许可证（带存储）