Small
·
2023
·Vol. 19
·期 29
·2207436
·
IF 13.3
Qing Yu Liu,Ying Wu,Zhen Qi Bu,Min Xia Quan,Jiao Yang Lu,Wei Tao Huang
Bimetallic nanomaterials (BNMs) have been used in sensing, biomedicine, and environmental remediation, but their multipurpose and comprehensive applications in molecular logic computing and information security protection have received little attention. Herein, This synthesis method is achieved by sequentially adding reactants under ice bath conditions. Interestingly, Ag-Cr NPs can dynamically selectively sense anions and reductants in multiple channels. Especially, ClO − can be quantitatively detected by oxidizing Ag-Cr NPs with detection limits of 98.37 nM (at 270 nm) and 31.83 nM (at 394 nm). Based on sequential-dependent synthesis process of Ag-Cr NPs, Boolean logic gates and customizable molecular keypad locks are constructed by setting the reactants as the inputs, the states of the resulting solutions as the outputs. Furthermore, dynamically selective response patterns of the Ag-Cr NPs can be converted into binary strings to exploit molecular crypto-steganography to encode, store, and hide information. By integrating the three dimensions of authorization, encryption, and steganography, 3 in 1 advanced information protection based on Ag-Cr nanosensing system can be achieved, which can enhance the anti-cracking ability of information. This research will promote the development and application of nanocomposites in the field of information security and deepen the connection between molecular sensing and the information world.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2023
·
IF 4.4
Haidong Teng,Kaitong Chen,Lan Wang,Jianping Wu,Gang Xu
l -syn-3-[4-(methylsulfonyl)] phenylserine is a key industrial intermediate for the synthesis of florfenicol, thiamphenicol , and other products. l -Threonine aldolase can synthesize l -syn-3-[4-(methylsulfonyl)] phenylserine from glycine and 4-methylsulphonyl benzaldehyde directly. However, the selectivity of free l -threonine aldolase was difficult to meet the requirement of industrialization. Here, we selected the suitable site to insert His tag as immobilized site to improve the selectivity of l -threonine aldolase based on the molecular dynamics simulation and prior protein engineering. The phenanthroline was coupled to the surface of magnetic nanoparticles coated with silicon and thus enabled the selective and oriented immobilization of His-tagged l -threonine aldolase. Due to magnetic nanoparticles coated with silicon as supports, it was easy to separate the residual reactant and impurities from the immobilized enzyme by magnetic attraction. Moreover, the immobilization was combined with the purification of free enzymes through directional immobilization, which avoided heterozyme linking supports and improved the utilization efficiency of supports. Our study demonstrates an effective approach: how to analyze and select enzyme-targeted ligation sites in conjunction with previous modifications of engineered bacteria, and the immobilized enzyme obtained also provided a good candidate for the industrial production of l -syn-3-[4-(methylsulfonyl)] phenylserine.
✓ 已验证
产品关联
DOI
高影响因子
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
·
2023
·
IF 6.7
Yong-Jun Huang,Xiao-Lin Zhong,Yi-Peng Zang,Ming-Han Yang,Jing Lin,Wei-Min Chen
The natural prenylated chalcone isobavachalcone ( IBC ) shows good antibacterial activity against Gram-positive bacteria but is ineffective against Gram-negative bacteria, most likely due to the outer membrane barrier of Gram-negative bacteria. The Trojan horse strategy has been shown to be an effective strategy to overcome the reduction in the permeability of the outer membrane of Gram-negative bacteria. In this study, eight different 3-hydroxy-pyridin-4(1 H )-one-isobavachalcone conjugates were designed and synthesized based on the siderophore Trojan horse strategy. The conjugates exhibited 8- to 32-fold lower minimum inhibitory concentrations (MICs) and 32- to 177-fold lower half-inhibitory concentrations (IC 50 s) against Pseudomonas aeruginosa PAO1 as well as clinical multidrug-resistant (MDR) strains compared to the parent IBC under iron limitation. Further studies showed that the antibacterial activity of the conjugates was regulated by the bacterial iron uptake pathway under different iron concentration conditions . Studies on the antibacterial mechanism of conjugate 1b showed that it exerts antibacterial activity by disrupting cytoplasmic membrane integrity and inhibiting cell metabolism. Finally, conjugate 1b showed a lower cytotoxic effects on Vero cells than IBC and a positive therapeutic effect in the treatment of bacterial infections caused by Gram-negative bacteria PAO1. Overall, this work demonstrates that IBC can be delivered to Gram-negative bacteria when combined with 3-hydroxy-pyridin-4(1H)-ones as siderophores and provides a scientific basis for the development of effective antibacterial agents against Gram-negative bacteria.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Yujie Chen,Ruhui Liu,Jingyan Li,Yanjie Wang,Chunfeng Guo,Xin Lü
1-Oleoyl-2-palmitoyl-3-linoleoylglycerol (OPL) is the most abundant triacylglycerol (TAG) molecule in Chinese human milk. The selectivity of three lipases including Rhizomucor miehei lipase (RML), Thermomyces lanuginose lipase (TL), and Candida antarctica lipase (CALB) in OPL synthesis were evaluated. The effects of different reaction conditions on selectivity were first investigated. Unexpectedly, the three sn -1,3 regioselectivity lipases, also have selectivity for the structure of product TAGs. Compared with symmetric TAGs, lipases had poor selectivity toward asymmetric OPL. Furthermore, molecular simulations were used to deeply understand the molecular factors affecting lipase selectivity. The results showed that hydrophobic interactions, lipases lid domain flexibility, and the stability of hydrogen bonds between lipases and TAGs were the main molecular factors responsible for the selectivity of lipases.
✓ 已验证
产品关联
DOI
高影响因子
Antioxidants
·
2022
·Vol. 12
·期 1
·64
·
IF 7.675
Ke Wang,Yan Li,Yimin Zhang,Jingxin Sun
The deterioration of wooden breast myofibrillar protein (WBMP) causes a decline in its processing performance, and the protein becomes easier to oxidize. Previous studies have revealed that the use of (-)-epigallocatechin-3-gallate (EGCG) may improve the physicochemical properties and oxidative stability of proteins in aqueous solutions. The effects of varying concentrations (0.01%, 0.02%, 0.03%, and 0.04%w/v) of EGCG on the physicochemical properties of a WBMP emulsion (1.2% WBMP/10% oil) and the inhibition of lipid and protein oxidation were studied. The results revealed that a moderate dose of EGCG (0.03%) could significantly (p< 0.05) improve the emulsion activity index (4.66 ± 0.41 m2/g) and emulsion stability index (91.95 ± 4.23%), as well as reduce the particle size of the WBMP emulsion. According to the micrographs and cream index, 0.03% EGCG retarded the phase separation by stopping the aggregation of droplets and proteins, thus significantly improving the stability of WBMP emulsions. During storage at 50 °C for 96 h, 0.03% EGCG inhibited lipid oxidation (lipid hydroperoxide and 2-thiobarbituric acid-reactive substance formation) and protein oxidation (carbonyl formation and sulfhydryl loss). In contrast, lower and higher EGCG concentrations (0.01%, 0.02%, and 0.04%) demonstrated shortcomings (such as weak antioxidant capacity or protein over-aggregation) in improving the quality and oxidation stability of the emulsion. In conclusion, a moderate dose of EGCG (0.03%) can be used to improve the quality and shelf life of WBMP emulsions.Keywords:wooden breast;myofibrillar protein;emulsion;(-)-epigallocatechin-3-gallate;physicochemical properties;oxidative stability
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of the Taiwan Institute of Chemical Engineers
·
2022
·
IF 5.477
Shangyan Zhou,Wei Liao,Zhengcheng Wang,Qian Zhou,Jin Long,Meida Chen,Qingmei Wang
Background Developing an economical and durable high-activity Pt-based electrocatalyst to alleviate the slow kinetics of the oxygen reduction reaction (ORR) is crucial for fuel cells. The shape-controllable Pt 3 Ni alloy catalyst with high electrocatalytic properties is regarded as one of the most efficacious electrocatalysts capable of accelerating ORR kinetics. Methods Here, sub-3 nm Pt-rich Pt 3 Ni dodecahedrons (DCs) has been developed via surfactant-driven shape evolution route as effective electrocatalysts for ORR. Significant findings The Pt 3 Ni DCs exhibit 4.3-fold improvement for mass activity and 4.7-fold improvement for specific activity respect to pristine Pt/C catalyst. Moreover, the remaining specific activity on Pt 3 Ni DCs is 6.7 times larger relative to pristine Pt/C catalyst after 20,000 scanning cycles. Such electrocatalytic performance enhancement is attributed to the ensemble effect including the dodecahedrons shape and synergistic effect between Pt and Ni atoms. We believe that our synthetic approach would provide new avenues toward the formation of polyhedron structures.
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 47
·期 5
·2257-2263
·
IF 3.925
Shiyu Nie,Fengfeng Chen,Tianbo Shen,Tingting Wang,Ningyu Di,Junkuo Gao
Adjusting the internal porous environment of MOFs to enhance their adsorption properties is considered an effective strategy to improve their gas separation performance. Herein, 1-ethyl-3-methyl-imidazolium acetate ([Emim][Ac]) was encapsulated in UiO-66 by an impregnation method for the improvement of carbon dioxide (CO2) capture performance. The synthesis and detailed characterizations of the developed [Emim][Ac]@UiO-66 are reported, and the effect of the loading amount of [Emim][Ac] on the CO2 separation performance was also studied. The obtained [Emim][Ac]@UiO-66 could efficiently show the synergistic effect of the adsorption of CO2 between [Emim][Ac] and UiO-66. The optimized [Emim][Ac]@UiO-66 exhibited a much higher CO2 adsorption capacity at 298 K and 1.0 bar, affording 2 times the CO2 uptake than its original UiO-66. In addition, the CO2/N2 selectivity and CO2/CH4 selectivity of [Emim][Ac]@UiO-66-3.5% are significantly higher than those of UiO-66. This study for designing various MOF-encapsulated ionic liquids potentially paves the way for the development of highly efficient absorbents for gas separations.
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2022
·Vol. 11
·期 24
·4073
·
IF 5.561
Yingrui Ji,Dongming Lan,Weifei Wang,Kok Ming Goh,Chin Ping Tan,Yonghua Wang
With the prevalence of edible diacylglycerol (DAG) oil, which is beneficial to human, the generation of 3-monochloropropanediol esters (3-MCPDE) and glycidyl esters (GE) as well as the stability of physical properties during heat-induced processing still need to be explored. In this study, the experiment used olive-based edible oil with different contents of DAG (40, 60, and 80%) to make crackers and fry chicken. They were heated at 160 and 180 °C to determine the changes in 3-MCPDE and GE, the crackers’ hardness and gumminess, and the physical properties of the oil. During baking and frying, 3-MCPDE decreased, while the content of GE slightly increased with the prolonged heating duration. Finally, 3-MCPDE and GE were lower than 1.25 mg/kg and 1.00 mg/kg, respectively. The AV increased proportionally as duration increased and POV was below 0.30 g/100 g. In general, the changes in 3-MCPDE and GE were related to the heating temperature and duration, and not significantly (p> 0.05) related to the content of DAG.Keywords:3-MCPD ester;glycidyl ester;diacylglycerol;crackers;frying oil
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2022
·Vol. 57
·期 45
·21009-21019
·
IF 4.682
Lin Lingyin,Zhou Yanfang,Chen Piaoyi,Xiao Qinglin,Ye Guodong
Transcatheter arterial embolization is an established treatment for hepatocellular carcinoma (HCC). However, the existing embolic materials still have some shortcomings, e.g., particle clumping can cause catheter occlusion. In this study, after the synthesis of monomer glyceryl triallyl ether monomer (GE), amorphous embolic agent (PGE) was synthesized by photopolymerization in the presence of GE and photoinitiator. Combined with RT-IR, TG and GPC, we verified that the polymerization followed a photo-driven radical-mediated [3 + 2] cyclopolymerization mechanism (PRMC) through experimental and modeled NMR. The macromolecular properties such as polymerization profile and morphology were studied by TG and SEM–EDS. Finally, the degradation, swelling and migration behavior of PGE were characterized, which indicated that PGE could embolize HCC by blocking the blood supply. In this study, PRMC prevented GE polymerization from degradative chain transfer with 70% yield in an hour, and 200–350 µm PGE was synthesized using this mechanism suitable for clinical use. Graphical abstract
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Xiaoya Tao,Cheng Chen,Yaochang Li,Xinguang Qin,Haizhi Zhang,Yuanyuan Hu,Zhengqi Liu,Xiaoming Guo,Gang Liu
Protein-polysaccharide-polyphenol delivery systems function as a promising tool to deliver bioactive ingredients aiming to improve their solubility and bioavailability. In this study, whey protein isolate (WPI), short-chain inulin (SCI), and cyanidin-3-glucoside (C3G) were first used to stabilize Pickering emulsions. The physicochemical properties and stability of curcumin encapsulated or not in Pickering emulsions were explored. Results showed that glycosylation and C3G reduced surface and interfacial tension on protein surfaces and inhibited the aggregation of emulsion droplets, thereby reducing the emulsion's particle size. WPI-SCI/C3G stabilized Pickering emulsion had the best stability. The CLSM results showed that the WPI-SCI and WPI-SCI/C3G stabilized emulsions were uniformly dispersed, suggesting that glycosylation and the interaction between protein and C3G enhanced the adsorption capacity of the interfacial protein and improved the stability of the Pickering emulsion. The retention rates of curcumin-loaded WPI-SCI- (67.34 %) and WPI-SCI/C3G- (77.07 %) stabilized Pickering emulsions on day 8 of storage were higher than those in WPI- (33.97 %) and WPI/C3G- (37.02 %) stabilized emulsions, and the degradation half-life was also extended from 7 days to >15 days. These findings provide a theoretical basis for the application of WPI Pickering emulsion and indicate a useful means for the delivery of bioactive components.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL THERMODYNAMICS
·
2023
·
IF 2.6
Mingcheng Li,Xiaojie Liu,Chunying Zhu,Taotao Fu,Xiqun Gao,Youguang Ma
Densities and viscosities were measured for binary solutions MEA (monoethanolamine) + 3DMA1P (3-dimethylamino-1-propanol), 3DMA1P + H 2 O and ternary solutions MEA + 3DMA1P + H 2 O at P = 0.101 MPa, T = 293.15 K ∼ 333.15 K. Based on the experimental densities of solutions, the volume properties of the excess molar volume, partial molar volume, apparent molar volume and limiting partial molar volume were calculated. Moreover, the viscosity deviation and viscous flow activation energy were calculated based on the experimental viscosity. Additionally, the intermolecular interactions of the mixed solutions were analyzed and discussed, and the Redlich-Kister equation was used to correlate the excess molar volume and viscosity deviation of the binary solutions.
✓ 已验证
产品关联
DOI
高影响因子
CELLULAR SIGNALLING
·
2023
·
IF 4.8
Hao Zhou,Jiangbo Huang,Fang Wang
Background Studies are ongoing to examine the versatile functions of circular RNAs (circRNAs) in human diseases. This research investigates the effects of hsa_circ_0000644 (circ_644) and its related molecules on the malignant behavior of bladder cancer (BCa) cells. Methods Abundant bioinformatics analyses were performed to screen the key circRNA and its related molecules in BCa. Tumor tissues and the para-tumorous tissues were collected from 58 patients with BCa. Expression of RUNX family transcription factor 3 (RUNX3), circ_644, microRNA-143-3p (miR-143-3p), and musashi RNA binding protein 2 (MSI2) in BCa tissues or cells was determined. Molecular interactions were confirmed by chromatin immunoprecipitation, RNA pull-down, and luciferase assays. Gain and loss-of function assays were performed using two BCa cell lines (T24 and HT1376). Results Circ_644 was highly expressed whereas RUNX3 , which could suppress circ_644 transcription, was lowly expressed in BCa tissues and cells. Upregulation of RUNX3 suppressed proliferation, colony formation, migration and invasion, and tumorigenicity of BCa cells and induced cell cycle arrest . However, the tumor-suppressive effects of RUNX3 were blocked by circ_644 upregulation. Circ_644 served as a sponge for miR-143-3p, and miR-143-3p bound to MSI2 mRNA. The rescue experiments showed that miR-143-3p inhibition or MSI2 overexpression restored the malignant behaviors of BCa cells induced by circ_644 knockdown or RUNX3 overexpression. Conclusion This study demonstrates that transcriptional activation of circ_644 upon RUNX3 downregulation drives the malignant development of BCa through the miR-143-3p/MSI2 axis. RUNX3 restoration or specific inhibition of circ_644 or MSI2 may help block BCa progression.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）