INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Yue Zhu,Teng Chen,Xiujuan Wan,Guiqin Zhu,Zhiyong Chen,Zhoujie Yang,Jing Yang,Tingting Feng,Zuhua Wang,Ying Zhou
Persistent intraoperative hemorrhage is the major cause of trauma-related mortality, emphasizing the need for hemostatic dressings with improved efficacy. In this study, we designed a PLGA-CTS-TXA composite sponge, which was fabricated by grafting of poly (lactic- co -glycol acid) (PLGA) and hemostatic small molecule tranexamic acid (TXA) onto chitosan (CTS), for rapid hemostasis. The sponge integrates the advantages of CTS, PLGA and TXA, showcasing superior porosity (nearly 85 %) and water absorption capacity (3197 ± 105 % within 30 s). It also demonstrates good biocompatibility (hemolysis <1.2 %, RCV > 125 %), low blood clotting index (12.4 %) and abbreviated clotting times (<30 s). The incorporation of TXA substantially augmented the hemostatic capability of the composite sponge. Compared to CTS alone, PLGA-CTS-TXA demonstrated a significantly reduced hemostatic time in both rat tail and mouse liver hemostatic models. Its hemostatic mechanism was further analyzed from the perspective of the actual interface created by the interaction between the material and the biological fluid (ie., protein corona). The results demonstrated that the swift hemostatic mechanism involves modulation of erythrocyte and platelet aggregation/adhesion and fibrin degradation, etc. , thereby influencing the coagulation cascade. This research extends the utility of TXA in hemostasis and offers novel idea into the development and hemostatic mechanism of new high-efficiency hemostatic dressings.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Xiawei Zhao,Gaoshuai Zhang,Shuwen Zhang,Rui Liu,Meijing Zhang,Biao Wang,Ye Zhang,Jianhui Liu,Hao Wang
Hydrogel dressings have emerged as promising tools for wound healing applications. However, the complex chemical synthesis and high cost of components, such as cytokines and stem cells, in most existing designs challenge their practical application. In this study, we adopted a modular design approach and natural materials to overcome these limitations, dividing the hydrogels into three distinct functional modules. The base module consisted of an interpenetrating polymer network of gelatin and chitosan. The drug release module incorporated liposomes loaded with quercetin, while the auxiliary module utilized a complex of protocatechuic aldehyde and trivalent iron. The presence of Schiff base crosslinking and hydrogen bond formation between the three modules enhanced the self-healing properties and mechanical strength of the hydrogel. Notably, the modular hydrogel exhibited excellent antioxidant, antibacterial, macrophage differentiation, and collagen promotion capabilities. This multifunctional hydrogel demonstrated the potential to provide safe and effective treatment, accelerate wound repair processes, and serve as a promising dressing for diabetic wound management. By utilizing natural components and a modular design strategy, this hydrogel offers a practical and cost-effective solution for improving wound healing outcomes, particularly in drug-resistant and diabetic wounds.
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Nanomaterials
·
2024
·Vol. 14
·期 23
·1873
·
IF 4.4
Elena V. Svirshchevskaya,Valentina V. Kostenko,Anna A. Boyko,Maxim Shevtsov,Roman V. Kholodenko,Maria V. Grechikhina,Iuliia A. Gracheva,Alexey Yu. Fedorov,Alexander M. Sapozhnikov
Anti-cancer targeted therapy is a promising approach. However, the identification of target molecules over-expressed in a wide range of tumors remains a significant challenge. The aim of this study was to analyze the expression of cell membrane-exposed heat shock protein 70 kDa (mHSP70) on different tumor cells and to develop a nanoscale delivery system based on a monoclonal antibody (mAb) that recognizes mHSP70 and uses chitosan core–shell nanoparticles (NPs). Several types of tumor cells (breast, pancreas, colon, prostate cancers, and some lymphomas) expressed mHSP70 as was determined by flow cytometry and confocal microscopy both in 2D and 3D cultures. Core NPs were formed by chitosan (C) conjugated to allocolchicinoid, which was used as a model drug (D). mAbs (A) targeting mHSP70 were complexed with succinylchitosan and used as NP shells forming final CAD-NPs. These NPs were characterized by size, charge, and functional activity. CAD-NPs were shown to have additional toxicity in comparison with CD-NPs in mHSP7-positive cells. Taken collectively, this study shows that mAb to mHSP70 can be used as a targeting vector in antitumor therapy.
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FOOD CHEMISTRY
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2025
·
IF 9.8
Zi-Qiang Luo,Tao Peng,Ya Hu,Bin-Yang Yang,Jing Wang,Tao Zou
The selection of suitable porous materials as the adsorbent of mycotoxin is of great significance for food safety. In this work, hierarchical porous materials with high specific surface area and suitable pore size distribution were prepared by one-step carbonization method using chitosan and KMnO 4 as carbon precursors and activators, respectively. The best adsorption performance was achieved using the hierarchical porous materials prepared at carbonization temperature of 600 °C. The adsorption capacity and Brunauer-Emmett-Teller specific surface area was 8.4 μg/mg and 1800.76 m 2 g −1 , respectively. The adsorption of patulin on hierarchical porous materials conforms to the Langmuir and pseudo-second-order model. Moreover, the hierarchical porous materials did not have a significant effect on the nutrients of the apple juice. The adsorbents prepared herein has been found to be a good solution for the adsorption of patulin, owing to their easy preparation, low cytotoxicity, high efficiency, and good reusability.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Yicheng Yang,Qinan Huang,Lipei Miao,Qihong Zhang,Chongyang Wu,Weike Su,Jingbo Yu
Crocetin (CT), a natural carotenoid dicarboxylic acid known for its high medicinal value, faces challenges such as poor water solubility, low bioavailability and instability, limiting its therapeutic applications. To overcome these challenges, this study introduces a novel Pickering emulsion gel stabilized by naturally sourced chitosan (CS) and γ -polyglutamic acid ( γ -PGA) nanoparticles (CS/ γ -PGA NPs) as a potential transdermal carrier for CT. The CS/ γ -PGA NPs were synthesized using an electrostatic crosslinking method pretreated by mechanical ball-milling, producing uniform and stable nanoparticles. These NPs stabilized a CT-loaded Pickering emulsion gel (CT-PE) with cornmint oil as the oil phase, achieving up to 98.53 % encapsulation efficiency. The CT-PE exhibited enhanced rheological properties, storage stability, sustained and targeted in vitro transdermal release, potent antioxidant activity, and effective antimicrobial effects with no cytotoxicity. Microstructural analysis revealed a dense network of aggregated particles forming a moderately strong gel structure, wherein encapsulated CT enhances emulsion stability through interaction with the gel network. The synergy between CS and γ -PGA facilitated a biocompatible and efficient carrier system, markedly boosting CT's therapeutic effectiveness. These results highlight CT-PE's potential as an effective transdermal carrier for CT, promising for future dermatological treatments and drug delivery systems.
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JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY
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2025
·
IF 1.9
Qifan Yu,Yifei Li,Yuxin Wang,Hanyu Zhang,Menghua Qi,Liangjie Song,Lei Chen,Zhishan Bai,Bingjie Wang
Multichannel integrated microfluidic technology retains the precise control characteristics of microfluidics. This technology is expected to replace traditional droplet preparation techniques for the batch production of adsorption materials with high performance and high monodispersity. In this study, porous chitosan microspheres (PCSM) were successfully synthesized using a self-developed modularized microfluidic system. As-prepared PCSMs were employed in adsorption separation of methyl orange (MO). The results proved that the average droplet size of PCSMs was 892 μm with the lowest coefficient of variation (CV), only 2.75%, while, the droplet flux was up to 210 mL·h−1. Batch static adsorption experiments showed that the saturated adsorption capacity of the adsorbent reached 215.26 ± 2.22 mg·g−1. Besides, the adsorption kinetics and equilibrium adsorption isotherm fitted pseudo-second-order kinetic model and Langmuir isotherm model well, respectively, indicating that the adsorption process is dominated by chemical reaction. Moreover, continuous adsorption experiment showed that under the conditions of initial solution concentration of 40 mg·L−1, bed diameter of 15 mm and filling height of 9.3 mm, the breakthrough time was 140 min, and the maximum adsorption capacity was up to 233.3 ± 4.30 mg·g−1. In addition, PCSMs exhibited stable regeneration performance and efficient selective adsorption for MO dyes in recycling (73.4% reabsorption productivity after 5 cycles). This study can provide theoretical support for the microfluidic batch synthesis and industrial application evaluation of high-performance adsorbents.
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2021
·
IF 5.268
Can Wang,Bingxian Lin,Yunren Qiu
A novel modified polysulfone (PSF) is successfully prepared for hemodialysis by grafting with a well-defined heparin-like polymer, sulfonated dihydroxypropyl chitosan (SDHPCS), which is obtained in proper sequence via alkalization of chitosan, etherification and sulfonation . PSF is modified via chloroacetyl chloride , and then, the chloroacylated polysulfone (CAPSF) with pristine PSF is transformed into CAPSF/PSF blend membrane via the phase inversion, followed introducing amino group into CAPSF on the surface and taking glutaraldehyde as bridge between modified PSF membrane and SDHPCS. The result of 1 H NMR spectrum of prepared CAPSF indicates that the degree of the substitution of chloroacetyl group . The SEM, EDS mapping, FTIR and XPS show that SDHPCS-g-PSF membranes are successfully prepared. The hydrophilicity of the membrane modified by SDHPCS is improved obviously, and the contact angle remarkably reduced from 87 ° to below 45°, exhibiting much better hydrophilicity. The hemocompatibility characterizations including BSA adsorption, Plasma recalcification time (PRT), hemolysis ratio (HR), activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT) also certificates that SDHPCS-g-PSF possesses lower BSA adsorption and enhanced blood compatibility.
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JOURNAL OF POLYMER RESEARCH
·
2022
·Vol. 29
·期 10
·1-17
·
IF 3.061
Shikhani Amani,Karam Sami,Said Majd,Atassi Yomen,Sarhan Hala
In this work, a hemostatic hydrogel is synthesized from biodegradable and biocompatible natural resources, namely chitosan and lactic acid. Without harsh chemicals as catalysts, the graft polymerization of lactic acid onto the chitosan backbone is carried out utilizing a domestic microwave to stimulate the grafting reaction, and create a physically cross-linked hydrogel. Structural characterization of the prepared hydrogel is performed using Fourier transformation infrared spectroscopy (FTIR) and X-ray diffraction (XRD), whereas morphological changes are determined using scanning electron microscopy (SEM). In vitro and in vivo tests are conducted to investigate the hemostatic performance of the hydrogel. The results of In vitro results reveal the effectiveness of the hydrogel in hemostasis, where it exhibits a significant low value of blood coagulation index (3.3%), a remarkable decrease in blood clotting time (110 s), and a good ability to adsorb red blood cells (45.6%) in comparison with the control samples. Furthermore, the findings of blood coagulation index and erythrocytes adsorption tests are remarkably better than those of some commercially available hemostatic products (GelSpon and Gas Hydrophel bandage). In addition, the in vivo test on mice shows that the hydrogel reduces blood coagulation time (58 s) in comparison with the regular physiological conditions without using any hemostatic material (93 s).
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Polymers
·
2022
·Vol. 14
·期 11
·2286
·
IF 4.967
Meiyan Wu,Wangfang Deng,Yidong Zhang,Chao Chen,Zhexuan Liu,Pedram Fatehi,Bin Li
It is highly desirable to develop a safe, highly efficient, and biodegradable drug carrier with an enhanced drug transport efficiency. Cellulose nanofibrils (CNF) and chitosan (CS) composite hydrogels are promising candidate carriers with biological compatibility and non-cytotoxicity. Herein, the CNF/CS composite beads were prepared by dissolving cellulose and CS in LiBr molten salt hydrate and regenerating in ethanol. This preparation method is facile and efficient, and the obtained porous CNF/CS beads with the weight ratio of 8:2 exhibited a large specific surface area, uniform micro-nano-sized pores, strong mechanical property, and water absorption-resistance. Moreover, these beads as drug (tetracycline hydrochloride, TH) carriers showed a higher encapsulation efficiency (47.4%) at the TH concentration of 5 mg/mL in 24 h, and a higher drug loading rate (12.0%) than pure CNF and other CNF/CS beads prepared with different ratios. In addition, the TH releasing behavior of CNF/CS (8:2) beads fitted well into the zero-order, first-order, and Higuchi models under an acid condition, indicating that the drug release of these pH-sensitive beads was mainly affected by drug concentration under an acid condition. Therefore, these CNF/CS beads have great potential to be used as drug carriers for medical applications.Keywords:cellulose nanofibrils;chitosan;drug carrier;tetracycline hydrochloride;drug release
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Polymer Chemistry
·
2022
·Vol. 13
·期 12
·1751-1762
·
IF 5.364
Yan Wang,Yuyong Ai,Xiaolin Liu,Bowei Chen,Yong Zhang
Herein, a novel indole-modified cross-linked chitosan aerogel (IAA-CTSA) was fabricated by grafting 3-indoleacetic acid onto chitosan and adding glutaraldehyde as a crosslinking agent through a facile two-step one pot method. The uptake properties and mechanism of uranium (U(VI)) by the constructed IAA-CTSA were discussed. IAA-CTSA was discovered to be a powerful and effective adsorbing material for the capture of U(VI) at pH 6.5. Equilibrium was achieved within 6 h, and the adsorption process accorded well with the pseudo-second-order kinetic model, inferring the chemical adsorption was the rate controlling step. The maximum capture capacity for U(VI) reached 847.5 mg g−1 calculated from the Langmuir model at 289 K. The removal efficiency for U(VI) of IAA-CTSA was strongly pH-dependent but insensitive to ionic strength. Furthermore, the removal efficiency of IAA-CTSA for U(VI) was maintained above 80% after 4 successive cycles using HCl and NaOH as eluents. The practical potential was tested using simulated U(VI) wastewater and illustrated that the resulting IAA-CTSA could be a competitive candidate for the elimination of U(VI) in simulated water environments. FTIR and XPS results illustrated that complexation, electrostatic and cation–π interactions were involved in the capture of U(VI). Briefly, the IAA-CTSA possesses good application prospects for wastewater remediation.
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RSC Advances
·
2019
·Vol. 9
·期 11
·6287-6298
·
IF 3.049
Yuanfeng Wei,Jianjun Zhang,Yazhen Zheng,Yaxiang Gong,Meng Fu,Chengran Liu,Liang Xu,Changquan Calvin Sun,Yuan Gao,Shuai Qian
The present study aims to develop cubosomes with surface cross-linked chitosan for sustained drug delivery and enhanced oral bioavailability of vinpocetine (VPT). GMO based liquid cubosomes with VPT loading were prepared by the high pressure homogenization method. In order to enhance the anti-digestion effect, chitosan was cross-linked on cubosomes by the Schiff reaction, followed by solidification via spray drying. The obtained spray-dried cubosomes (chito-cubosomes) are spherical microspheres with nano-sized holes on the surface. After reconstitution, the particle size and zeta potential of chito-cubosomes were determined to be ∼250 nm and +35.9 mV, respectively. In comparison to unmodified liquid cubosomes, chito-cubosomes exhibited a significant anti-digestion effect with a typical sustained release profile. In comparison to a VPT suspension, liquid cubosomes showed a 2.5-fold higher Cmax and 3.0-fold higher AUC0–∞, while chito-cubosomes further enhanced bioavailability (5.0-fold) with prolonged MRT (2.2-fold) and delayed Tmax (2.8-fold). The results suggested that chito-cubosomes could be a promising drug carrier for enhancing oral absorption with sustained release behavior.
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Desalination and Water Treatment
·
2016
·
IF 1.272
Manfeng Li,Zhaomei Wang,Bingjie Li
A cellulose/chitosan hydrogel bead was prepared by extruding and regenerating the blends from ionic liquid 1-ethyl-3-methylimidazolium acetate ([Emim]Ac) in ethanol. FT-IR, scanning electron microscopy (SEM) and X-ray powder diffraction techniques were used to prove the successful blending of both natural polymers (cellulose and chitosan). Brunauer–Emmet–Teller surface area and pore diameter analysis showed that the beads were nanoporous with pores measuring from 10 to 20 nm. Batch adsorption experiments demonstrated that the bead had a maximum adsorption capacity of 40 mg/g for congo red (CR) dye removal from aqueous solutions, which was more efficient than the most reported natural biosorbents. The CR adsorption capacity at equilibrium was related to initial CR concentration, dosage of beads and pH values of the CR solution. When the initial concentration of CR was 30 mg/L, and the adsorbent dosage was 2.0 g/L, the equilibrium of adsorption was reached within 115 min with the removal rate of 89.6%. The Langmuir model represented the adsorption isotherm data and the experimental results followed a pseudo-second-order rate model, indicating that intraparticle diffusion dominated the adsorption process. The cellulose/chitosan hydrogel beads can be used as an efficient adsorbent for dye contaminant removal from wastewater.
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