WATER RESEARCH
·
2025
·
IF 12.4
Shuangshuang Cheng,Rui Cui,Yuehong Sun,Yuxin Li,Yangjian Zhou,Zilong Song,Xin Yang
The degradation kinetics and mechanism of four free bases were comparatively investigated during UV/H 2 O 2 and UV/PDS water treatment processes. The UV/PDS system demonstrated superior removal efficiency for all four bases compared to the UV/H 2 O 2 process. Pyrimidine bases (cytosine and thymine) exhibited notably faster degradation rates than purine bases (adenine and guanine) in both advanced oxidation systems. Guanine (G) was then selected as a representative free base to investigate the internal and external reverse-reduction process. The time-resolved transient spectra shows that key intermediate product G(-H) • was formed in both processes, where this radical play critical role in initiating reverse-reduction process. During UV/H 2 O 2 process, the G’s antioxidant intermediates (e.g. 8-oxoG) trigger an internal reverse-reduction process, which can reduce G(-H) • back to their parent compounds. The second-order rate constant for the reaction between of G(-H) • and 8-oxoG was quantitatively determined to be (2.60 ± 0.12) × 10 8 M −1 s −1 . Conversely, during the UV/PDS process, the reverse-reduction process of G(-H) • is predominantly governed by external inhibition mediated by dissolved organic matter (DOM), with no competing internal pathway observed. Specifically, intrinsic intermediate products generated during UV/H 2 O 2 treatment initiate an internal reverse-reduction process, effectively suppressing DOM-driven external reduction. This may also explain why the degradation rates of free bases were lower in the UV/H₂O₂ system than in the UV/PDS system, and why DOM exhibited a stronger inhibitory effect on their degradation in the UV/PDS process compared to UV/H₂O₂. DFT calculations were conducted to verify the proposed mechanisms, which were applicable to the four studied bases. Studying both internal and external reverse-reduction processes can significantly enhance the understanding of compounds transformation pathways and enhance the accuracy of predicting degradation rates in real-world aqueous systems.
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高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Shuang Chen,Yujie Liu,Fei Zhai,Min Jia
A novel label-free fluorescence aptasensor was established for chloramphenicol (CAP) detection by DNA four-arm junction-assisted target recycling and SYBR Green I dye-aided fluorescence-signal amplification. The CAP aptamer was hybridized to its complementary strand (primer) to form a double-stranded primer/aptamer complex. In the presence of CAP, aptamers can specifically bind with CAP to dissociate primers, which can trigger the self-assembly of four hairpins to continuously generate DNA four-arm junctions. After digesting the excess hairpins using T7 exonuclease, SYBR Green I was inserted into the base pair-rich DNA four-arm junctions, which led to a significant increase in fluorescence intensity. Under optimal conditions, the developed aptasensor can detect CAP in a linear range of 1.0 pg mL −1 to 10 ng mL −1 with a detection limit of 0.72 pg mL −1 . The recovery rates in milk and honey ranged from 90.3% to 106.6%. Thus, the method shows substantial potential for CAP detection in food products.
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高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 12
·1-11
·
IF 6.408
Wang Yue,Zhai Hongguo,Yin Jiaqi,Guo Qi,Zhang Yuhao,Yang Qingqing,Li Falan,Sun Xia,Guo Yemin,Zhang Yanyan
With the development of exonuclease, the exonuclease has been used to construct a variety of aptasensor and to realize the signal amplification. Among them, based on silver nanoparticles (Ag NPs) and exonuclease I (Exo I)-assisted cycle signal amplification strategy, we designed a novel high-sensitivity dual-target electrochemical biosensor to detect Pb 2+ or Hg 2+ in water. In the presence of Hg 2+ , the Hg 2+ was fixed to the aptamer chain by thymine-Hg 2+ -thymine (T-Hg 2+ -T), resulting in the decrease of signal. When Pb 2+ was present, DNA single strand S2 dissociated and was bound to Pb 2+ , which automatically triggered Exo I to selectively cut the single chain from the recognition site to achieve the cyclic amplification of the electrochemical signal. The interaction between aptamer and Exo I was investigated by gel electrophoresis. Under the optimum conditions in the scan range -0.20 to 0.60 V, the biosensor had high sensitivity with a linear range of 100 pg/L to 10.0 mg/L, Pb 2+ or Hg 2+ , and the detection limits were 17.0 pg/L ( R 2 = 0.993) and 12.0 pg/L ( R 2 = 0.993), respectively. The relative standard deviation (RSD) of the sensor was 0.5–2.6%, and the recovery of spiked standard solutions was between 98.3 and 110%. The cycle amplification strategy supported by this enzyme has promising applications in detection of the two metal ions in various fields. Graphical Abstract
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Chao Jiang,Yansheng Li,Hui Wang,Desheng Chen,Yongqiang Wen
We proposed a facile DNA-based hydrogel capillary sensor (DHCS) for sensitive detection of Pb 2+ . This proposed biosensor took advantage of the capillary action of the capillary tube controlled by the hydrogel film and the mesh size of the hydrogel based on target-responsive DNA. In the presence of Pb 2+ , the crosslinker substrate strand was cleaved leading the hydrogel fractured partly, and the mesh size of the hydrogel film plugging the end of the capillary tube changed. Owing to this, the behavior of the solution flowing through the capillary controlled by the hydrogel film was affected by the concentration of Pb 2+ . Therefore, through reading the distance and duration time by the naked eye, quantitative detection of Pb 2+ was achieved without any equipment. Additionally, and importantly, the tiny Pb 2+ responsive DNA hydrogel (0.02 μL) can be directly applied to analysis without encapsulation operations of nanoparticles or enzymes. Benefiting from the DNAzyme merits of high sensitivity and selectivity, in the range of 0.01 –50 μM, the developed method was able to detect as low as 10 nM Pb 2+ in less 1 h with the distance under 20 mm, meanwhile, the signals of blank and other high concentration metal ions were negligible. This sensor could be utilized to detect Pb 2+ in tap water with good reliability. Therefore, this proposed strategy provided a miniature, portable, sensitive and highly selective, visual platform for the detection of Pb 2+ in situ measurement.
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CRYSTENGCOMM
·
2012
·Vol. 15
·期 1
·206-212
·
IF 3.842
Xin-Yu Zhao,Ying-Jie Zhu,Feng Chen,Bing-Qiang Lu,Jin Wu
In this paper, a surfactant-free rapid microwave-assisted hydrothermal synthesis of hydroxyapatite nanosheet-assembled flower-like hierarchical nanostructures (NFHNs) is reported. The effects of the experimental conditions on the morphology and crystal phase of the product are investigated. A possible formation mechanism of hydroxyapatite NFHNs is proposed. The morphology of the product can vary from flower-like to polyhedra by adjusting the microwave heating temperature. The protein and DNA adsorption properties of the as-prepared hydroxyapatite NFHNs are studied. The loading capacities of the as-prepared hydroxyapatite NFHNs for bovine serum albumin (BSA), hemoglobin (Hb) and fish sperm DNA are determined to be 165, 164 and 112 mg g−1, respectively. The protein release process is conducted at different pH values (pH 7.2, 5.5 and 4.8) in phosphate buffer saline (PBS), and the pH-controlled protein release behavior has been found. Thus, the as-prepared hydroxyapatite NFHNs are promising for protein drug delivery applications.
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APPLIED ORGANOMETALLIC CHEMISTRY
·
2018
·Vol. 32
·期 8
·e4423
·
IF 3.581
XueWen Liu,Jie Huang,YuXuan Tang,YouMing Shen,JiLin Lu
A new polypyridyl ligand containing a nitro group and two new ruthenium complexes of it were synthesized. The two complexes exhibited non-dppz DNA ‘light switch’ effects after interaction with calf thymus DNA. Introducing both electron-withdrawing group (─ NO 2 ) and electron-donating group (─ CH 3 ) may be the reason that the two complexes display DNA ‘light switch’ behaviors. Furthermore, one of the complexes showed higher photocleavage activity, topoisomerase I inhibition activity and DNA affinity than the other. The present work shows that the more active complex can be employed as a non-dppz DNA ‘light switch’, DNA photocleaver and topoisomerase I inhibitor. In addition, the two complexes have no or weak cytotoxic activities against Eca-109 and A549 cells.
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ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
M.S. Bacchu,M.R. Ali,S. Das,S. Akter,H. Sakamoto,S.-I. Suye,M.M. Rahman,K. Campbell,M.Z.H. Khan
An efficient platform for the detection of Salmonella enterica serovar Typhi ( S. Typhi ) is essential for early-stage diagnosis of typhoid to prevent and contain outbreaks. Here, we fabricated an electrochemical DNA biosensor for selective identification of S. Typhi in real samples. The biosensor has been fabricated by immobilizing an amine labelled S. Typhi specific single-strand capture probe on the surface of gold nanoparticles (AuNP) and poly cysteine (P-Cys) modified screen-printed electrode. Differential pulse voltammetry (DPV) of anthraquinone-2-sulfonic acid monohydrate sodium salt (AQMS) as a signal indicator was monitored to detect S. Typhi by hybridization of target DNA with the probe DNA . The fabricated biosensor shows a detection range of 1 × 10 −6 to 1 × 10 −22 molL −1 with a LOD of 6.8 × 10 −25 molL −1 in S. Typhi complementary linear target and 1.8 × 10 5 to 1.8 CFUml −1 with a LOD of 1 CFUml −1 in a real S. Typhi sample. The biosensor shows excellent discrimination ability to some bases mismatched and different bacterial cultures (same and distant genera). The most beneficial points of the proposed DNA biosensor are the lower limit of detection and the ability to reuse the biosensor more than 6 to 7 times. In addition, the practicability of the biosensor was investigated via detecting S. Typhi in blood, poultry feces, egg, and milk whereby excellent recoveries ranging from 96.54 to 103.47% were demonstrated indicating that this biosensor might be the most promising diagnostic tool for monitoring S . Typhi in clinical and food samples.
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BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Wei Wang,Ting Bao,Xi Zeng,Huayu Xiong,Wei Wen,Xiuhua Zhang,Shengfu Wang
In this work, a novel and ultrasensitive electrochemical biosensor was constructed for DNA detection based on functionalized gold clusters/graphene nanohybrids (AuNCs/GR nanobybrids) and exonuclease III (Exo III)-aided cascade target recycling. By utilizing the capacity of GR as universal template, different metal nanoclusters including AuNCs/GR nanobybrids and PtNCs/GR nanohybrids were synthesized through convenient ultrasonic method. Exo III-aided cascade recycling was initiated by target DNA, generating the final cleavage product (S2), which acted as a linkage between capture probe and the functionalized metal nanoclusters/GR conjugates in the construction of the biosensor. The AuNCs/GR-DNA-enzyme conjugates acted as interfaces of enzyme-catalyzed silver deposition reaction, achieving DNA detection ranging from 0.02 fM to 20 pM with a detection limit of 0.057 fM. In addition, PtNCs/GR-DNA conjugates presented peroxidase-like activity and the functionalized PtNCs/GR nanohybrids-based electrochemical biosensor also realized DNA detection by catalyzing the 3,3′,5,5′-tetramethylbenzidine-hydrogen peroxide (TMB-H 2 O 2 ) system to produce electrochemical signal. This metal clusters/GR-based multiple-amplified electrochemical biosensor provided an universal method for DNA detection.
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高影响因子
ANALYTICAL SCIENCES
·
2018
·Vol. 35
·期 4
·441-448
·
IF 1.355
Wang Xiaojing,Yang Mei,Liu Qingyan,Yang Siyi,Geng Xintong,Yang Yixia,Fa Huanbao,Wang Yongzhong,Hou Changjun
In this paper, an ultrasensitive electrochemical biosensor based on carboxylated multi-walled carbon nanotube/molybdenum disulfide composites (MWCNTs-COOH/MoS 2 ) for the detection of KRAS gene is described. An easy, low-cost method, named one-step hydrothermal, was used for the synthesize of MWCNTs-COOH/MoS 2 nanocomposites, and scanning electronic microscopy (SEM), high resolution transmission electron microscopy (HRTEM), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) were used for characterizing the prepared composites. Furthermore, cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were employed for an electrochemical performance study of this biosensor. Under optimal conditions, the detection limit of target DNA achieved down to 3 fM ( S / N = 3) with high sensitivity; the linear range with the logarithm of the concentrations of target DNA varied from 1.0 × 10 −14 to 1.0 × 10 −7 M. Finally, the practicality of our proposed sensor was verified by a determination of the KRAS gene in human serum samples with good accuracy and high precision due to the excellent conductivity and large active surface area of the MWCNTs-COOH/MoS 2 nanocomposites. This proposed biosensor thus provides a practical method for the rapid and sensitive analysis of gene detection.
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Advanced Science
·
2020
·Vol. 7
·期 13
·2000684
·
IF 15.84
Jinpeng Han,Yuchen Cui,Xinpeng Han,Chenyu Liang,Wenguang Liu,Dan Luo,Dayong Yang
Engineering dynamic systems or materials to respond to biological process is one of the major tasks in synthetic biology and will enable wide promising applications, such as robotics and smart medicine. Herein, a super-soft and dynamic DNA/dopamine-grafted-dextran hydrogel, which shows super-fast volume-responsiveness with high sensitivity upon solvents with different polarities and enables creation of electric circuits in response to microbial metabolism is reported. Synergic permanent and dynamic double networks are integrated in this hydrogel. A serials of dynamic hydrogel-based electric circuits are fabricated: 1) triggered by using water as switch, 2) triggered by using water and petroleum ether as switch pair, 3) a self-healing electric circuit; 4) remarkably, a microbial metabolism process which produces ethanol triggering electric circuit is achieved successfully. It is envisioned that the work provides a new strategy for the construction of dynamic materials, particularly DNA-based biomaterials; and the electric circuits will be highly promising in applications, such as soft robotics and intelligent systems.
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Acta Pharmaceutica Sinica B
·
2021
·
IF 11.614
Fangfang Wang,Sora Jin,Franklin Mayca Pozo,Danmei Tian,Xiyang Tang,Yi Dai,Xinsheng Yao,Jinshan Tang,Youwei Zhang
DNA damage response (DDR) is a highly conserved genome surveillance mechanism that preserves cell viability in the presence of chemotherapeutic drugs. Hence, small molecules that inhibit DDR are expected to enhance the anti-cancer effect of chemotherapy. Through a recent chemical library screen, we identified shikonin as an inhibitor that strongly suppressed DDR activated by various chemotherapeutic drugs in cancer cell lines derived from different origins. Mechanistically, shikonin inhibited the activation of ataxia telangiectasia mutated (ATM), and to a lesser degree ATM and RAD3-related (ATR), two master upstream regulators of the DDR signal, through inducing degradation of ATM and ATR-interacting protein (ATRIP), an obligate associating protein of ATR, respectively. As a result of DDR inhibition, shikonin enhanced the anti-cancer effect of chemotherapeutic drugs in both cell cultures and in mouse models. While degradation of ATRIP is proteasome dependent, that of ATM depends on caspase- and lysosome-, but not proteasome. Overexpression of ATM significantly mitigated DDR inhibition and cell death induced by shikonin and chemotherapeutic drugs. These novel findings reveal shikonin as a pan DDR inhibitor and identify ATM as a primary factor in determining the chemo sensitizing effect of shikonin. Our data may facilitate the development of shikonin and its derivatives as potential chemotherapy sensitizers through inducing ATM degradation.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Chunying Li,Chunying Wei
We have developed a label-free fluorescent probe using DNA-templated silver nanoclusters (Ag NCs). The DNA-Ag NCs probe was a simple, non-toxic, and sensitive method and could be used to detect biothiols and the activity of acetylcholinesterase (AChE). The fluorescent intensity of DNA-Ag NCs was quenched significantly in the prescence of biothiols, which might be attributed to the formed nonfluorescent complex between Ag NCs and biothiols, the aggregation of Ag NCs, and the efficient energy transfer from DNA-Ag NCs to thiol group. The cysteine (Cys) and glutathione (GSH) could be detected as low as 0.134 μM and 0.172 μM, respectively, within a good linear range from 0–5.0 μM ( R 2 = 0.991) for Cys, and 0.5–4.5 μM ( R 2 = 0.983) for GSH. In addition, the proposed method could also detect selectively the activity of AChE in the range from 0–4 U/L, with a detection limit of 0.071 U/L. We believed that the proposed approach laid a foundation for the researchers in bioimaging, pharmacology, diagnostics, and other fields.
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