ADVANCED FUNCTIONAL MATERIALS
·
2016
·Vol. 27
·期 4
·1604258
·
IF 11.382
Zhifang Ma,Xiaodan Jia,Jing Bai,Yudi Ruan,Chao Wang,Jianming Li,Mengchao Zhang,Xiue Jiang
Premature leakage of photosensitizer (PS) from nanocarriers significantly reduces the accumulation of PS within a tumor, thereby enhancing nonspecific accumulation in normal tissues, which inevitably leads to a limited efficacy for photodynamic therapy (PDT) and the enhanced systematic phototoxicity. Moreover, local hypoxia of the tumor tissue also seriously hinders the PDT. To overcome these limitations, an acidic H 2 O 2 -responsive and O 2 -evolving core–shell PDT nanoplatform is developed by using MnO 2 shell as a switchable shield to prevent the premature release of loaded PS in core and elevate the O 2 concentration within tumor tissue. The inner core SiO 2 -methylene blue obtained by co-condensation has a high PS payload and the outer MnO 2 shell shields PS from leaking into blood after intravenous injection until reaching tumor tissue. Moreover, the shell MnO 2 simultaneously endows the theranostic nanocomposite with redox activity toward H 2 O 2 in the acidic microenvironment of tumor tissue to generate O 2 and thus overcomes the hypoxia of cancer cells. More importantly, the Mn(ΙΙ) ion reduced from Mn(ΙV) is capable of in vivo magnetic resonance imaging selectively in response to overexpressed acidic H 2 O 2 . The facile incorporation of the switchable MnO 2 shell into one multifunctional diagnostic and therapeutic nanoplatform has great potential for future clinical application.
研究领域:
生物医学工程
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肿瘤治疗学
·
纳米医学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Hongyu Chen,Qiujun Lu,Kaili He,Meiling Liu,Youyu Zhang,Shouzhuo Yao
We report a novel facile upconversion fluorescent and colorimetric dual-readout assay for H 2 O 2 as well as H 2 O 2 -related analytes via enzyme-controlled cyclic signal amplification strategy. As a proof of application demonstration, the highly sensitive detection of choline and acetylcholine chloride (ACh) was performed. This sensing strategy involves the reaction of ACh with acetylcholinesterase (AChE) to generate choline which is followed catalytically oxidized by choline oxidase (ChOx) to produce H 2 O 2 . The generated H 2 O 2 can transform Fe 2+ to Fe 3+ and free hydroxyl radical ( OH). The as-produced Fe 3+ and OH can simultaneously oxidize o -phenylenediamine (OPD) to 2,3-diaminophennazine (OPDox) and the Fe 3+ was reduced to Fe 2+ . The Fe 2+ produced from the redox processes between Fe 3+ and OPD could be cycle used to generate OPDox that can significantly quench upconversion nanoparticles (UCNPs) fluorescence based on inner filter effects (IFE) and cause a visible change from colorless to yellow color. The phenomenon is further designed as a colorimetric logic gate. Moreover, we also detected H 2 O 2 , glucose, lactate and uric acid (UA) with the higher sensitivity by utilizing the assay strategy, which revealed the extensive applications of the proposed sensing strategy in biomedical analysis.
研究领域:
生物化学
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分析化学
·
医学诊断
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产品关联
DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Fei Qu,Qianqian Yang,Bingjie Wang,Jinmao You
Recently, the aggregation-induced emission (AIE) of nanoclusters triggered by metal ions has been received great attentions. However, the good AIE efficiency usually requires excessive metal ions, which may result in an undesired competition between metal ions and targets. In this work, by the synergistic effect of Pb 2+ and Zr 4+ , a fewer amounts of metal ions can induce more aggregates of glutathione-capped Cu nanoclusters (CSH-CuNCs), resulting in a higher AIE efficiency. Next, by virtue of the oxidative property of H 2 O 2 , the AIE of GSH-CuNCs-Pb 2+ -Zr 4+ system quenches linearly with the concentration of H 2 O 2 from 1 to 60 μmol/L. Moreover, many biological substrates, such as glucose and cholesterol, can generate H 2 O 2 in the presence of their specific oxidases and O 2 . Therefore, the detection of glucose or cholesterol can also be achieved by the proposed method, and the limits of detection of glucose and cholesterol are 0.37 and 2.7 μmol/L, respectively. Finally, this method has been validated to be sensitive and selective for glucose or cholesterol detection in human serum samples.
研究领域:
分析化学
·
生物医学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Min Zhang,Jing Zheng,Jianping Wang,Jingli Xu,Tasawar Hayat,Njud S. Alharbi
In recent years, the fabrication of noble metal functionalized magnetic carbon nanotubes for a variety of novel biosensors has aroused considerable interest owing to their synergistic improved sensitivity. In the paper, the Fe 3 O 4 and Cu nanoparticles (NPs) were integrated into the NCNTs to obtain N-doped carbon@Fe 3 O 4 -Cu nanotubes (NCNTs@Fe 3 O 4 @Cu) through a one-pot high temperature decomposition procedure. Then, Au NPs were assembled on the magnetic NCNTs to obtain NCNTs@Fe 3 O 4 @Au composite by galvanic replacement with Cu NPs. The resultant composite had provided a friendly platform for enzyme immobilization to develop highly sensitive biosensors. After the cytochrome c(cyt c) was accumulated by NCNTs@Fe 3 O 4 @Au composite, the cyt c/NCNTs@Fe 3 O 4 @Au gathered to the surface of electrode with an external magnet. Thus, the sensitivity of designed biosensor could be further improved, and most of electrochemical interferences could be excluded. Benefiting from the unique hybrid structure of the composite, the direct electron transfer of cyt c was highly facilitated and the constructed biosensors exhibited good performance for the detection of H 2 O 2 with an extraordinary low detection limit of 0.3 μM, which revealed potential application for fabricating novel electrochemical biosensors.
研究领域:
生物传感技术
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材料科学
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电化学分析
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2018
·
IF 5.667
Jing Chen,Yunjing Gao,Qin Ma,Xiaoyan Hu,Yali Xu,Xiaoquan Lu
In the present work, the classical synthesis method of 5,10,15,20-(4-sulphonatophenyl) porphyrin (TPPS 4 ) was improved. The synthesis process became more environmentally friendly, and the yield was increased by 10%. We constructed a sensitive fluorescent sensor for detection hydrogen peroxide (H 2 O 2 ) and glucose (C 6 H 12 O 6 ) by using the hyperfluorescence of TPPS 4 . The TPPS 4 -Fe 3+ complex system of the sensor could generate electron transfer process (ET) in the process of hydrogen peroxide (H 2 O 2 ) and glucose oxidation, which quenched the TPPS 4 fluorescence. The system had a linear range of 0–42 μM for hydrogen peroxide, and the minimum detectable limit (LOD) was 1.24 nM (S/N = 3). Compared with other detection methods, the proposed method had the advantages of higher sensitivity, lower detection limit and more simple operation.
研究领域:
生物化学
·
分析化学
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2022
·
IF 14.224
Meijie Liu,Zipeng Xing,Huanan Zhao,Sijia Song,Yichao Wang,Zhenzi Li,Wei Zhou
The fabrication of an efficient photoFenton system without the addition of H 2 O 2 is still a challenge and is cost-effective and favorable for practical applications. In this work, a core@shell Z-scheme heterojunction nanoreactor was successfully fabricated, in which hierarchical two-dimensional (2D) ZnIn 2 S 4 nanosheets are coated on defective iron-based metal-organic frameworks (MOFs) (NH 2 -MIL-88B(Fe)), realizing efficient in-situ evolution of H 2 O 2 and constructing an optimal heterogeneous Fenton platform. The degradation rates of defective NH 2 -MIL-88B(Fe)@ZnIn 2 S 4 (0.4 g L −1 ) for bisphenol A and ofloxacin under visible light irradiation within 180 min reached 99.4% and 98.5%, respectively, and the photocatalytic hydrogen production efficiency was approximately 502 μmol h −1 g −1 . The excellent photoFenton performance was attributed to the introduction of ligand defects into the MOF, which can adjust the band structure to enhance the light absorption capacity, and the in-situ generation of H 2 O 2 accelerating the Fe 3+ /Fe 2+ conversion. In addition, the formation of the core@shell nanoreactor Z-scheme heterojunction structure promoted spatial charge separation. This strategy offers new ideas for constructing efficient photocatalysis and photoFenton systems.
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Yu Wang,Yuxin He,Yujing Chi,Peiji Yin,Lishan Wei,Wenwen Liu,Xinyao Wang,Han Zhang,Haiyan Song
Photocatalytic H 2 O 2 production is a green and sustainable technology, which still exposes the issues of excessive dependence on organic electron donors and pure O 2 , and lacking effective use. It is necessary to develop more efficient and more economical photocatalysis system for H 2 O 2 production. Here, we construct a S-scheme p-n heterojunction from the p-type MnS and the n-type protonated g-C 3 N 4 (PCN) semiconductors for photocatalytic H 2 O 2 production and achieve the in situ use of H 2 O 2 for oxidative degradation of oxytetracycline (OTC). The PCN/MnS composite synthesized by a one-step method well maintains a nanosphere structure of α-MnS and load the PCN on the sphere surface to form a heterojunction with strong interaction. PCN/MnS exhibits the improved photogenerated charge separation and electron transfer efficiency. An optimal photocatalyst can produce 669.6 μM of H 2 O 2 for 6 h without using the electron donors and the pure O 2 gas. The mechanism proposed the contribution of a S-scheme p-n heterojunction forms between PCN and MnS. The electrons in conduction band (CB) of MnS are responsible for the major single-electron reduction of •O 2 - radicals and the auxiliary two-electron reduction of O 2 for H 2 O 2 production, while the holes in valence band (VB) of PCN further promote the separation of photocarriers. The photogenerated H 2 O 2 can be effectively used as an in-situ oxidant to achieve 82.2% degradation of OTC in water within 80 min. The •O 2 - radicals which originate from the in situ decomposition of H 2 O 2 over PCN/MnS mainly contribute to the OTC oxidation .
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DOI
高影响因子
Frontiers in Chemistry
·
2021
·
IF 5.221
Zhang Zhikun,Zhao Wenmeng,Hu Cuixia,Cao Yapeng,Liu Yumin,Liu Qingju
L-Histidine (L-His) is an essential amino acid, which is used to synthesize proteins and enzymes. The concentration of L-His in the body is controlled to regulate tissue growth and repair of tissues. In this study, a rapid and sensitive method was developed for colorimetric L-his detection using Cu2+ ions to inhibit the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB)–H2O2 system. H2O2 can oxidize TMB to oxTMB in the presence of copper, and the change in color from colorless (TMB) to blue (oxTMB) is similar to that observed in the presence of peroxidase. However, because the imidazole ring and carboxyl group of L-His can coordinate with Cu2+ ions to form stable L-His–Cu2+ complexes, the color of the TMB–H2O2 solution remains unchanged after the addition of L-His. Therefore, because L-His effectively hinders the colorimetric reaction of TMB with H2O2, this assay can be used to quantitatively determine the concentration of L-His in samples. Under optimized conditions, our colorimetric sensor exhibited two linear ranges of 60 nM to 1 μM and 1 μM to 1 mM for L-His detection and a detection limit of 50 nM (S/N = 3); furthermore, the assay can be performed within 20 min. Moreover, the proposed assay was used to determine the concentration of L-His in urine samples, suggesting that this convenient and label-free colorimetric method presents promising applications in bioanalytical chemistry and clinical diagnosis.
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2024
·
IF 4.1
Ting Yu,Ying Liang,Zejun Zhou,Fengjie Chen,Yingjun Wang,Yunjun Mei,Mengxi Cao,Bolei Chen,Pu Wang,Yawei Wang
The photocatalytic generation of hydrogen peroxide (H 2 O 2 ) using artificial synthetic methods is promising. However, the sluggish hole-induced water oxidation half-reaction that hinders charge separation severely limits H 2 O 2 generation efficiency. This study used the oxidation half-reaction of tetracycline (C 22 H 24 N 2 O 8 , TC) to accelerate charge separation over GaOOH/In(OH) 3 composites with Type II heterojunctions under sunlight irradiation to increase the H 2 O 2 generation efficiency. The results demonstrated that the H 2 O 2 generation efficiency significantly increased from 74.7 to 389.8 µmol L −1 h −1 as the TC degradation efficiency increased from 0.02 to 0.37 mg L −1 h −1 . Radical quenching experiments further indicated that TC degradation consumed holes, increasing the electron formation rate and enhancing H 2 O 2 generation. A comparison with density functional theory calculations revealed that H 2 O 2 generation was a one-step 2-electron route (O 2 + 2e − + 2H + → H 2 O 2 ). These findings are expected to provide new strategies for promoting photocatalytic synthesis of H 2 O 2 .
研究领域:
环境科学
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化学
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材料科学
·
光催化
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 5
·1-11
·
IF 5.833
He Yanlei,Li Ning,Liu Xiangwei,Chen Wei,Zhu Xixi,Liu Qingyun
Urchin-like peroxidase mimics 5,10,15,20-tetrakis (4-carboxyl phenyl) porphyrin–functionalized CuCo 2 O 4 nanospheres (Por-CuCo 2 O 4 ) has been fabricated as an excellent visual biosensor. X-ray diffractometry (XRD), scanning electron microscopy (SEM), and X-ray photoelectron spectroscopy (XPS) have been employed to characterize the composition, morphologies, and elemental analysis of the as-synthesized Por-CuCo 2 O 4 . The catalytic activity of Por-CuCo 2 O 4 was evaluated by the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) with the aid of H 2 O 2 , which exhibited a visual blue change with an absorption maximum at 652 nm for only 10 s. The peroxidase-like behaviors of Por-CuCo 2 O 4 conformed to the Michaelis-Menten equation. Electrochemistry, radical scavenger, and fluorescence probe experiments verified that electron transfer, •O 2 − radicals, and holes (h + ) are the important factors during the catalytic oxidation of TMB. Based on the inhibition of dopamine (DA) on TMB oxidation, the Por-CuCo 2 O 4 -based colorimetric biosensor has been successfully constructed for sensitive determination of DA witha detection limit (LOD) of 0.94 μΜ. In addition, colorimetry was validated to detect DA in serum samples with high sensitivity and good selectivity. Graphical abstract
5,10,15,20-tetrakis (4-carboxyl phenyl) porphyrin–functionalized urchin-like CuCo 2 O 4 (Por-CuCo 2 O 4 ) with excellent peroxidase activity, ascribed to the synergistic effect between •O 2 − radicals and holes (h + ). A fast colorimetric sensor on the basis of Por-CuCo 2 O 4 has been constructed to quantitatively determine dopamine concentration in human serums.
研究领域:
纳米技术
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生物传感器
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分析化学
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材料科学
·
生物化学
✓ 已验证
DOI
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2024
·Vol. 22
·期 1
·1-17
·
IF 10.6
Chen Wenting,Hu Fangfang,Gao Qian,Zheng Caiyun,Bai Que,Liu Jinxi,Sun Na,Zhang Wenhui,Zhang Yanni,Dong Kai,Lu Tingli
Chemodynamic therapy (CDT) based on intracellular Fenton reaction to produce highly cytotoxic reactive oxygen species (ROS) has played an essential role in tumor therapy. However, this therapy still needs to be improved by weakly acidic pH and over-expression of glutathione (GSH) in tumor microenvironment (TEM), which hinders its future application. Herein, we reported a multifunctional bimetallic composite nanoparticle MnO2@GA-Fe@CAI based on a metal polyphenol network (MPN) structure, which could reduce intracellular pH and endogenous GSH by remodeling tumor microenvironment to improve Fenton activity. MnO2 nanoparticles were prepared first and MnO2@GA-Fe nanoparticles with Fe3+ as central ion and gallic acid (GA) as surface ligands were prepared by the chelation reaction. Then, carbonic anhydrase inhibitor (CAI) was coupled with GA to form MnO2@GA-Fe@CAI. The properties of the bimetallic composite nanoparticles were studied, and the results showed that CAI could reduce intracellular pH. At the same time, MnO2 could deplete intracellular GSH and produce Mn2+ via redox reactions, which re-established the TME with low pH and GSH. In addition, GA reduced Fe3+ to Fe2+. Mn2+ and Fe2+ catalyzed the endogenous H2O2 to produce high-lever ROS to kill tumor cells. Compared with MnO2, MnO2@GA-Fe@CAI could reduce the tumor weight and volume for the xenograft MDA-MB-231 tumor-bearing mice and the final tumor inhibition rate of 58.09 ± 5.77%, showing the improved therapeutic effect as well as the biological safety. Therefore, this study achieved the high-efficiency CDT effect catalyzed by bimetallic through reshaping the tumor microenvironment.Graphical Abstract
研究领域:
癌症治疗
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纳米技术
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生物医学工程
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药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Royal Society Open Science
·
2022
·Vol. 9
·期 9
·
IF 3.653
Ye He,Panlin Wang,Xiaojing Chen,Yahuang Li,Jiajun Wei,Guoxi Cai,Kiyoshi Aoyagi,Wenxiang Wang
A simple strategy to rapidly detect glucose was developed by utilizing core (Fe3O4)–shell (Pt) magnetic nanoparticles (Fe3O4@Pt NPs) as a nanoenzyme and a paper-based colorimetric sensor. In the presence of H2O2, Fe3O4@Pt NPs catalyze the redox reaction of 3,3′,5,5′-tetramethylbenzidine (TMB) and generate a colour change from colourless to blue. On this basis, a colorimetric glucose sensing method assisted by glucose oxidase (GOx) was developed. Under the optimal conditions, the detection limits of the proposed assay for H2O2 and glucose were 0.36 µM and 1.27 µM, respectively. Furthermore, the fabricated colorimetric method was successfully applied to analyze glucose concentrations by using a paper device as a measuring platform without a spectrometer. In addition, this method exhibited satisfactory recovery for glucose detection in human serum samples and urine samples, which satisfied the requirements for normal detection of real samples. This study provides a good candidate for health monitoring of glucose and also expands the applications of nanoenzymes and paper-based colorimetric assays in point-of-care testing.
研究领域:
纳米技术
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分析化学
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生物传感器
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即时检测
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DOI
高影响因子
危险品化学品经营许可证（带存储）