Experimental and Therapeutic Medicine
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2023
·Vol. 26
·期 2
·1-12
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IF 2.7
Xiongfei Shi,Xingzhi Yu,Liping Yang,Xiaohua Duan
Gastrodia elata Blume is a traditional Chinese medicine with a long history, which has numerous pharmacological activities, such as anti‑inflammation, anti‑oxidation and protection of nerves. The present study investigated the regulatory effect of ethyl acetate extract of Gastrodia elata (EEGE) on the β‑amyloid (Aβ) toxicity of Caenorhabditis elegans (C. elegans). First, the main components of EEGE were analyzed using high‑performance liquid chromatography, and the total phenols, total flavonoids and total antioxidant capacity of EEGE were determined. Next, the regulation effect of EEGE on Aβ‑induced toxicity of C. elegans was evaluated through experiments on nematode paralysis, lifespan, oxidative and heat stress, locomotor ability, reproductive ability, reactive oxygen species (ROS) level, Aβ aggregation test, malondialdehyde (MDA) level, catalase (CAT) activity and superoxide dismutase (SOD) activity. Finally, the mechanism of EEGE was elucidated using RNA sequencing (RNA‑Seq) and the expression levels of related genes were verified using quantitative PCR. The present study revealed that the main components of EEGE included phosphorylated (p)‑hydroxybenzyl alcohol, p‑hydroxybenzaldehyde and 4,4'‑dihydroxydiphenylmethane, possessing strong in vitro free radical scavenging and reducing abilities. In addition, after the intervention of EEGE, the paralysis of nematodes could be delayed, the survival time of the nematodes was prolonged, the survival rate of the nematodes under stress (high temperature and oxidation) conditions was improved, the activity capacity and reproductive capacity of the nematodes were improved, the activities of SOD and CAT were improved and the levels of ROS and MDA were reduced. Notably, EEGE directly inhibited Aβ plaque aggregation in nematodes. RNA‑Seq analysis showed that EEGE regulated metabolism and longevity‑related genes, and these genes were regulated by the insulin/IGF‑1 signaling (IIS) pathway. Therefore, the present study hypothesized that the regulatory mechanism of EEGE was significantly related to the IIS pathway. The present research results demonstrated that the protective effect of EEGE on transgenic C. elegans was to reduce Aβ protein aggregation, improve the in vivo antioxidant level, effectively remove free radicals and to regulate the expression of genes related to IIS pathway, thereby reducing Aβ‑induced toxicity and delaying nematode paralysis.
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GOLD BULLETIN
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2023
·
IF 2.2
Hong Qing,Jin Xinchun,Zhou Chenheng,Shao Jiahui
Protein oligomerization contributes to Alzheimer’s disease development (AD). A nanoparticle that can speed up the oligomerization of proteins is generally considered harmful. Gold nanoparticles (AuNPs) have been reported to be making headway in biological platforms, but they may also have the capacity to stimulate protein oligomerization. Our goal herein was to investigate the neurotoxicity and oligomerization of amyloid-β-1-42 (Aβ 1-42 ) in the presence of AuNPs. The precipitation approach was used to create AuNPs, which were then analyzed using transmission electron microscopy (TEM), ThT, Congo red, and CD spectroscopy. The results demonstrated that the 50-nm-sized fabricated AuNPs guided acceleration in Aβ 1-42 . In addition, cytotoxicity studies on PC 12 cells showed that Aβ 1-42 with AuNPs were less toxic than untreated oligomers Aβ 1-42 in terms of inducing cell death, oxidative apoptosis, stress, and membrane leakage. In conclusion, our investigation sheds light on how AuNPs stimulate the development of cytotoxic oligomers by binding to proteins in Alzheimer’s disease.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2023
·
IF 8.6
Jia Pang,Hongbin Wang,Caiyan Zhang,Weidong Fan,Yang Feng,Liting Yu,Lili Fan,Rongming Wang,Zixi Kang,Daofeng Sun
β-cyclodextrin (β-CD), with intrinsic nano-cavity, is a promising monomer to construct thin-film composite (TFC) membranes for molecular separations. Nevertheless, interfacially polymerize β-CD into continuous membranes is challenging due to the low reactivity of hydroxyl groups. To address this issue, in this work, ionic liquid (IL) has been introduced into the interfacial polymerization (IP) process of β-CD and trimesoyl chloride (TMC), forming high-quality TFC membranes for organic solvent nanofiltration (OSN). Investigations have been done into how IL effects membrane structure and separation performance. In contrast to traditional β-CD-TMC TFC membrane, the β-CD-IL-TMC TFC membrane possesses upgraded crosslinking, continuity, stability, and OSN separation property. The best β-CD-IL-TMC membrane has an ethanol permeance of up to 57.01 L m −2 h −1 MPa −1 and Naphthol green B rejection rate of 99.98% and can sustain a stable separation performance within 20-hour test, indicating the fabricated β-CD-IL-TMC membranes have advantage of separating and purifying organic solvent.
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Journal of Advanced Research
·
2023
·
IF 10.7
Wenjuan Tang,Yu Hu,Kaihui Tu,Zhengyan Gong,Man Zhu,Tianfeng Yang,Ammar Sarwar,Bingling Dai,Dongdong Zhang,Yingzhuan Zhan,Yanmin Zhang
Introduction Tumor-associated calcium signal transducer 2 (Trop2) has been used as a transport gate for cytotoxic agents into cells in antibody–drug conjugate designs because of its expression in a wide range of solid tumors. However, the specific role of Trop2 itself in breast cancer progression remains unclear and small molecules targeting Trop2 have not yet been reported. Objectives To screen small molecules targeting Trop2, and to reveal its pharmacological effects and the molecular mechanisms of action. Methods Small molecule targeting Trop2 was identified by cell membrane chromatography, and validated by cellular thermal shift assay and point mutation analyses. We investigated the pharmacological effects of Trop2 inhibitor using RNA-seq, human foreskin fibroblast (HFF)-derived extracellular matrix (ECM), Matrigel drop invasion assays, colony-forming assay, xenograft tumor model, 4T1 orthotopic metastasis model and 4T1 experimental metastasis model. The molecular mechanism was determined using immunoprecipitation, mass spectrometry, immunofluorescence, immunohistochemistry and Western blotting. Results Here we identified Bruceine D (BD) as the inhibitor of Trop2, and demonstrated anti-metastasis effects of BD in breast cancer. Notably, Lys307 and Glu310 residues of Trop2 acted as critical sites for BD binding. Mechanistically, BD suppressed Trop2-induced cancer metastasis by blocking the formation of Trop2/β-catenin positive loop, in which the Trop2/β-catenin complex prevented β-catenin from being degraded via the ubiquitin-proteosome pathway. Destabilized β-catenin caused by BD reduced nucleus translocation, leading to the reduction of transcription of Trop2, the reversal of epithelial-mesenchymal transition (EMT) process, and the inhibition of ECM remodeling, further inhibiting cancer metastasis. Additionally, the inhibitory effects of BD on lung metastatic colonization and the beneficial effects of BD on prolongation of survival were validated in 4T1 experimental metastasis model. Conclusions These results support the tumor-promoting role of Trop2 in breast cancer by stabilizing β-catenin in Trop2/β-catenin positive loop, and suggest Bruceine D as a promising candidate for Trop2 inhibition.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
·
IF 6.1
Zhen Qin,Sainan Yu,Kemin Zhang,Xiasen Wei,Junjie Li,Zheyi Zhang,Sibao Wan,Haiyan Gao
β-1,3-Glucan-degrading enzymes are widely used in fields such as food processing, plant protection, and breweries. In this work, we identified a glycoside hydrolase (GH) family 157 endo-β-1,3-glucanase (BsGlc157A) from Bacteroides sp. M27 and characterized its biochemical properties, structural model, and antifungal activity. Enzymological characterization indicated that BsGlc157A performs its optimal catalytic activity at pH 6.0 and 40 °C. BsGlc157A adopted the classic (β/α)8 TIM-barrel structure. Two catalytic residues, the nucleophile (Glu215) and the proton donor (Glu123), were confirmed via structural modeling and site-directed mutagenesis. Moreover, BsGlc157A hydrolyzed curdlan into a series of oligosaccharides with polymerization degrees 2–5 and exhibited inhibitory effects on the hyphal growth of typical fruit pathogenic fungi (Monilinia fructicola, Alternaria alternata, and Colletotrichum gloeosporioides), thereby illustrating effective biocontrol activity. These results revealed the catalytic properties and the application potential of GH family 157 β-1,3-glucanase, thus providing valuable biochemistry information about the group of carbohydrate-active enzymes.
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International Journal of Electrochemical Science
·
2023
·
IF 1.5
Chun Feng,Lijuan Zhu,Yaqiong Cao,Yin Di,Zongxue Yu,Guhui Gao
This study reports a new strategy for enhancing the anti-corrosion properties of epoxy coatings. For this purpose, beta-cyclodextrin-graphene oxide (β-CD-g-GO) nanosheets were synthesized via anchoring beta-cyclodextrin on a graphene oxide surface in the presence of 3-glycidoxypropyltrimethoxysilane (KH560). The structure of the material was characterized by SEM, EDX, XRD, FT-IR and TGA. The inclusion of 2 wt. % GO and β-CD-g-GO nanosheets in epoxy resin remarkably enhanced the corrosion protection performance of the material relative to that of pure epoxy coating. Electrochemical impedance spectroscopy (EIS) measurements revealed that the impedance of the epoxy coating was improved after the addition of 2 wt. % GO and β-CD-g-GO nanosheets. The most pronounced improvement in the properties of the epoxy coating was obtained using β-CD-g-GO nanosheets. Scratches tests confirmed the reduced steel substrate corrosion in the case of epoxy coating that included β-CD-g-GO nanosheets compared to the corrosion obtained for other samples.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
·
IF 8.2
Tongtong Hou,Xu Shao,Minling Ding,Kun Mei,Xin Wang,Ping Guan,Xiaoling Hu
Photoinduced modulation of A β 42 aggregation has emerged as a therapeutic option for treating Alzheimer's disease (AD) due to its high spatiotemporal controllability, noninvasive nature, and low systemic toxicity. However, existing photo-oxidants have the poor affinity for A β 42 , low depolymerization efficiency, and difficulty in crossing the blood-brain barrier (BBB), hindering their application in the treatment of AD. Here, through hydrophobic interactions and hydrogen bonding, we integrated the near-infrared (NIR) photosensitizer indocyanine green with transferrin (denoted as TF-ICG), a protein with a high affinity for A β 42 , and demonstrated its anti-amyloid activity in vitro. TF-ICG was shown to bind to A β 42 residues via hydrophobic interaction, impeding π-π stacking of A β 42 peptide monomers and disassembling mature A β 42 protofibrils in a concentration-dependent manner. More importantly, under NIR (808 nm, 0.6w/cm 2 ) irradiation, TF-ICG completely inhibited the fibrillation process of A β 42 to generate amorphous aggregates, with an inhibition rate of 96 % at only 65 nM. Meanwhile, TF-ICG could photo-oxidize rigid A β 42 aggregates and break them down into small amorphous structures. Tyrosine fluorescence assay further demonstrated the intrinsic affinity and targeting of TF-ICG to A β 42 fibrils. In vitro studies validated the anti-amyloid activity of TF-ICG, which provided a theoretical basis for further in vivo application as a BBB-penetrating nanotherapeutic platform.
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PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2023
·Vol. 25
·期 19
·13474-13486
·
IF 3.3
Xin Guan,Xiao-li Wang,Xue-wen Zhu,Hui Yu,Ming Yang,Xiang-ting Dong,Ying Yang,Long Xia
β-Bi2O3 demonstrates excellent photocatalytic activity under visible light, but it has a very high photogenerated e−–h+ recombination rate and quite low quantum efficiency. AgBr also shows excellent catalytic activity but Ag+ is easily reduced to Ag under light radiation, which limits its application in the photocatalysis field, and there are few reports about the application of AgBr in photocatalysis. In this study, the spherical flower-like porous β-Bi2O3 matrix was first obtained, and then the spherical-like AgBr was embedded between the petals of the flower-like structure to avoid direct light radiation. The only light through the pores on the β-Bi2O3 petals could be transmitted onto the surfaces of AgBr particles to form a nanometer point light source, which photo-reduced Ag+ on the surface of the AgBr nanospheres to construct the Ag-modified AgBr/β-Bi2O3 embedded composite and a typical Z-scheme heterojunction was constructed. Under this bifunctional photocatalyst and visible light, the RhB degradation rate reached 99.85% in 30 min, and the photolysis water hydrogen production rate reached 6.288 mmol g−1 h−1. This work is as an effective method for not only the preparation of the embedded structure, quantum dot modification and flower-like morphology but also for the construction of Z-scheme heterostructures.
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INDUSTRIAL CROPS AND PRODUCTS
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2023
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IF 5.9
Qiufeng Deng,Rui Zhai,Baohua Chen,Xiaoxiao Jiang,Haixiang Li,Chen Li,Mingjie Jin
Developing value-added lignin-based biomaterials for enzyme immobilization has drawn great attention. This study developed a cost-effective and eco-friendly one-pot strategy to synthesize the rod-like lignin@zeolitic imidazolate framework-8 (ZIF-8) hybrid nanomaterial by using lignin derived from lignocellulosic biofuel production. The incorporation of lignin into the ZIF-8 network not only altered the morphology, but also modified the surface properties of the material, making it an ideal support for enzyme immobilization. The synthesized nanoscale hybrid materials were used to immobilize beta-glucosidase (BG) with high immobilization capacity and about 92–166 mg/g of BG was immobilized through physical adsorption. The immobilized BG exhibited good stability, catalytic activity and recycling properties, and was reused under acidic conditions for more than 8 cycles with more than 60% activity kept. The as-prepared hybrid material can serve as a great carrier for immobilizing various biomolecules.
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Nanomaterials
·
2023
·Vol. 13
·期 5
·915
·
IF 5.3
Jing-Jing Yang,Ying-Zhuo Shen,Zheng Wang,Bo Zhou,Xiao-Ya Hu,Qin Xu
In this study, β-Bi2O3nanosheets functionalized with bisphenol A (BPA) synthetic receptors were developed by a simple molecular imprinting technology and applied as the photoelectric active material for the construction of a BPA photoelectrochemical (PEC) sensor. BPA was anchored on the surface of β-Bi2O3nanosheets via the self-polymerization of dopamine monomer in the presence of a BPA template. After the elution of BPA, the BPA molecular imprinted polymer (BPA synthetic receptors)-functionalized β-Bi2O3nanosheets (MIP/β-Bi2O3) were obtained. Scanning electron microscopy (SEM) of MIP/β-Bi2O3revealed that the surface of β-Bi2O3nanosheets was covered with spherical particles, indicating the successful polymerization of the BPA imprinted layer. Under the best experimental conditions, the PEC sensor response was linearly proportional to the logarithm of BPA concentration in the range of 1.0 nM to 1.0 μM, and the detection limit was 0.179 nM. The method had high stability and good repeatability, and could be applied to the determination of BPA in standard water samples.Keywords:β-Bi2O3;nanosheet;PEC sensor;bisphenol A;molecularly imprinted
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Small
·
2023
·Vol. 19
·期 30
·2300154
·
IF 13.3
Rong Tu,Xinyu Li,Qingfang Xu,Tenghua Gao,Xian Zhang,Bao-Wen Li,Song Zhang,Takashi Goto,Lianmeng Zhang
The oriented growth of β-Ga 2 O 3 films has triggered extensive interest due to the remarkable and complex anisotropy found in the β-Ga 2 O 3 bulks. Remarkable properties, including stronger solar-blind ultraviolet (SBUV) absorption, better mobility, and higher thermal conductivity, are usually observed along <010> direction as compared to other low-index axes. So far, <010>-oriented β-Ga 2 O 3 film growth has been hindered by the lack of suitable substrates and higher surface energy of the (010) crystal plane. Herein, the first growth of uniquely <010>-oriented β-Ga 2 O 3 films on quartz substrates by laser chemical vapor deposition (LCVD) are reported. By investigating the effects of deposition temperature ( T dep ) and O 2 flow rate ( R O2 ) on the growth of β-Ga 2 O 3 films, it is found that the formation of <010> orientation is closely related to the higher stability of oxygen close-packed planes under O-rich condition. As a result, a grain size of up to ≈2 µm and a deposition rate of up to ≈ 40 µm h −1 are obtained. Metal-semiconductor-metal (MSM) type detector based on <010>-oriented β-Ga 2 O 3 film exhibits ultra-fast response speed, 1–2 orders of magnitude higher than those of most detectors based on β-Ga 2 O 3 films with other orientations.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2023
·
IF 4.2
Xuejuan Wei,Xiaopeng Chen,Linlin Wang,Xiaojie Wei,Yang Yang,Jiaqi Liang,Jiezhen Liang
Isothermal vapor–liquid equilibrium data of the binary system (−)-α-pinene + (−)-β-pinene and the quaternary system (−)-α-pinene + (−)-β-pinene + water + phenoxyacetic acid were measured at 313.2, 323.2, and 333.2 K using headspace gas chromatography. The experimental data showed that the addition of phenoxyacetic acid and water promoted the separation of (−)-α-pinene and (−)-β-pinene. The experimental data were correlated using the UNIQUAC and NRTL models. The two models showed good correlation for the binary system, whereas the UNIQUAC model gave more accuracy for the quaternary system. Moreover, the COSMO-RS model can be an efficacious method for predicting VLE data of the determined binary and quaternary systems. In addition, the optimal spatial configuration and binding energy between the molecules were obtained by DFT, and the effects on (−)-α-pinene and (−)-β-pinene with the addition of phenoxyacetic acid and water were discussed on the molecular scale.
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