JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS
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2024
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IF 1.3
Lingjiao Wang,Mengqiang Zhao,Jiao Li,Hongtao Xu,Yang Song,Yuanyuan Zhao,Jing Yu,Chunhua Zhou
Background Therapeutic drug monitoring for antidepressants (ADs) is vital due to the potentially serious consequences and disputes related to medical events. Therefore, we created a quick and convenient analysis way for separation and quantification of ADs. Methods To ensure quantitative stability, we divided the 16 ADs or their metabolites into 4 pools (AD1-AD4), considering the hospital frequency that the clinician prescribed, the physicochemical properties of medicines, and the calibration range of selected ADs. After precipitation with methanol, the analytes were eluted for at least 3.5 min on a BEH C 18 analytical column by different gradient elution methods. Results: The LLOQ and LOD were 1.25–10 ng/mL and 0.42–5 ng/mL, respectively. High precision (<12 %) and accuracy (87.07–111.47 %) were demonstrated by quality control samples both within and between days. All the compounds were stable at room temperature and within −80 °C. Conclusion The method is of wide clinical and laboratory interest due to simpler sample cleanup, shorter chromatographic run times, and wider calibration range compared to other methods.
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高影响因子
CARBOHYDRATE RESEARCH
·
2025
·
IF 2.5
Wei Wang,Jiarong Zhu,Yizhou Wang,Liangkun Long,Qunying Lin,Jing Wang,Shaojun Ding
Two new ɑ -galactosidases PpAgl27B and PpAgl27C from Penicillium parvum 4–14 were functionally investigated in this study. Based on the analysis of catalytic domain and phylogenetic tree, PpAgl27B (435 aa) and PpAgl27C (543 aa) belong to glycoside hydrolase (GH) 27 family. After expression in Pichia pastoris , the recombinant PpAgl27B and PpAgl27C showed the highest activities at pH 3.5 and 65 °C, or 4.0 and 45 °C, respectively. Using p -nitrophenyl- α - d -galactopyranoside ( p NPGal) as substrate, the Michaelis constant were 0.90 mM for PpAgl27B and 2.54 mM for PpAgl27C. PpAgl27C had a low catalytic activity toward p NPGal and negligible activities on various natural substrates. Differently, PpAgl27B efficiently released galactose from the artificial substrate, raffinose family oligosaccharides, or galactomannans. Hydrolysis of corn bran arabinoxylan (CBAX) 1 or 2 were conducted by PpAgl27B alone or in combination with the enzyme blend E_CBAX1. PpAgl27B released a small amount of galactose (1.7–3.0 mg/g) from the both substrates. Compared with the individual enzymes, the liberations of galactose, xylose and arabinose from the substrates were significantly enhanced by combing PpAgl27B and E_CBAX1. The degrees of synergy of the enzyme combination for the saccharification of CBAX1 or CBAX2 were 1.20 and 1.13, respectively. PpAgl27B showed promising potential for the valorization of galactose-rich feedstocks as well as CBAX.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2024
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IF 4.9
Ning Wang,Xiaole Qi,Fengyan Xiao
Covalent organic frameworks (COFs), due to their unique structure and morphology, manifest great potential as active substrates to support noble metals for sustainable catalytic applications. However, it is still a challenge for efficient and effective catalysis using these kinds of COFs-based catalysts. Here, black phosphorus (BP) nanosheets modified spherical COFs (SCOFs) were synthesized, followed by the immobilization of Au nanoparticles (Au NPs) to fabricate Au NPs/BP/SCOFs microspheres . The as-prepared Au NPs/BP/SCOFs microspheres were then filled in a column for the continuous catalytic reduction of 4-nitrophenol. A fast and highly efficient flow-through catalysis of 4-nitrophenol with a reduction efficiency of 99 % under the flux of 2500 L m −2 h −1 was obtained. Furthermore, a stable catalytic performance under flow-through process was exhibited with only 0.13 wt% of total Au NPs releasing after testing for 24 h. The strategy for designing COFs-based substrates with enhanced electron transfer and controllable morphology would develop a new route to promote the application of these kinds of COFs-based catalysts.
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Journal of Energy Chemistry
·
2024
·
IF 14
Shicheng Deng,Songfan Wang,Yuanyuan Wang,Qian Xiao,Yuena Meng,Dongxing Kou,Wenhui Zhou,Zhengji Zhou,Zhi Zheng,Sixin Wu
Kesterite Cu 2 ZnSn(S,Se) 4 (CZTSSe) solar cells suffer from severe carrier recombination, limiting the photovoltaic performance. Unfavorable energy band alignment at the p-n junction and defective front interface are two main causes. Herein, oxygen incorporation in CZTSSe via absorber air-annealing was developed as a strategy to optimize its surface photoelectric property and reduce the defects. With optimized oxygen incorporation conditions, the carrier separation and collection behavior at the front interface of the device is improved. In particular, it is found that oxygen incorporated absorber exhibits increased band bending, larger depletion region width, and suppressed absorber defects. These indicate the dynamic factors for carrier separation become stronger. Meanwhile, the increased potential difference between grain boundaries and intra grains combined with the decreased concentration of interface deep level defect in the absorber provide a better path for carrier transport. As a consequence, the champion efficiency of CZTSSe solar cells has been improved from 9.74% to 12.04% with significantly improved open-circuit voltage after optimized air-annealing condition. This work provides a new insight for interface engineering to improve the photoelectric conversion efficiency of CZTSSe devices.
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Catalysts
·
2024
·Vol. 14
·期 3
·183
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IF 3.8
Jiarong Ma,Run Zhou,Yu Tu,Ruixin Ma,Daimei Chen,Hao Ding
Ni3Si2O5(OH)4rods (NS) were synthesized via a hydrothermal method, employing natural wollastonite as a template. The hierarchical Ni3Si2O5(OH)4rods exhibited vertically oriented nanosheets, resulting in a substantial increase in the specific surface area (from 2.24 m2/g to 178.4 m2/g). Subsequently, a CdS/Ni3Si2O5(OH)4composite photocatalyst (CdS/NS) was prepared using a chemical deposition method. CdS was uniformly loaded onto the surface of the Ni3Si2O5(OH)4nanosheets, successfully forming a heterojunction with Ni3Si2O5(OH)4. The CdS/NS photocatalyst in the presence of lactic acid as a sacrificial agent demonstrated an impressive H2production rate of 4.05 mmol h−1g−1, around 40 times higher than pure CdS. The photocorrosion of CdS was effectively solved after loading. After four cycles, the performance of CdS/NS remained stable, showing the potential for sustainable applications. After photoexcitation, electrons moved from Ni3Si2O5(OH)4to the valence band of CdS, where they interacted with the holes via an enhanced interface contact. Simultaneously, electrons in CdS transitioned to its conduction band, facilitating hydrogenation. The enhanced performance was attributed to the improved CdS dispersion by Ni3Si2O5(OH)4loading and efficient photogenerated carrier separation through the heterojunction formation. This work provides new perspectives for broadening the applications of mineral materials and developing heterojunction photocatalysts with good dispersibility and recyclability.
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APPLIED ORGANOMETALLIC CHEMISTRY
·
2018
·Vol. 32
·期 3
·e4208
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IF 3.581
Yan Gao,Jiasheng Fang,Yiwei Zhang,Chao Zhang,Shuo Zhao,Yuming Zhou,Mengqiu Huang,Xiaoli Sheng
A facile strategy is reported for the fabrication of Pt-loaded core–shell nanocomposite ellipsoids (Fe 2 O 3 -Pt@DSL) consisting of ellipsoidal Fe 2 O 3 cores, double-layered La 2 O 3 shells and deposited Pt nanoparticles (NPs). The formation of the doubled-shelled structure uses Fe 2 O 3 -Pt@mSiO 2 as template sacrificial agent and it involves the re-deposition of silica and self-assembly of metal oxide units. The preparation methods of double-shelled metal oxides avoid repeated coating and etching and could be utilized to fabricate other shaped double-shelled composites. Characterization results indicated that the Fe 2 O 3 -Pt@DSL nanocomposites possessed mesoporous structure and tunable shell thickness. Moreover, due to the formation of Fe 2 O 3 and La 2 O 3 composites, Pt NPs can also be stabilized via deposition on chemically active oxides with a synergistic effect. Therefore, as a catalyst for the reduction of 4-nitrophenol, Fe 2 O 3 -Pt@DSL showed superior catalytic activity and reusability due to structural superiority and enhanced composite synergy. Finally, well-dispersed Pt NPs were encapsulated into the void between the shell layers to construct the Fe 2 O 3 -Pt@DSL-Pt catalyst.
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FEMS MICROBIOLOGY LETTERS
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2018
·Vol. 365
·期 5
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IF 1.735
Ke Xia,Xia Xiao-Yuan,Zheng Ren-Chao,Zheng Yu-Guo
The Erg28p protein is localized to the endoplasmic reticulum, where it acts as a scaffold to tether the C-4 demethylase complex involved in the sterol biosynthesis pathway of Saccharomyces cerevisiae. However, due to the challenges involved in characterizing the interactions of membrane proteins, the precise region of Erg28p that is responsible for the assembly of this enzyme complex remains unknown. To address this question, mutants with serial truncations in the C-terminus of Erg28p were constructed based on a topology prediction of its transmembrane domain. Sterol profiles demonstrated that intermediates involved in the stepwise removal of the two C-4 methyl groups from the tetracyclic sterol ring were accumulated in the ERG28Δ135-447 strain. Homologous alignment of Erg28p further identified a highly conserved 10-amino acid sequence (63LS/QARTFGT/LWT72) within the truncated region of ERG28Δ136-273. Complementation of the BY4741/erg28 strain with the ScERG28Δ175-204 plasmid resulted both in a significant growth inhibition and a reduction of ergosterol biosynthesis compared with the plasmid without the Δ175-204 truncation. Furthermore, homology modeling of the Erg28p mutant indicated that the deletion of residues 63–72 significantly disrupted the 3D structure of the four parallel helices in Erg28p. Taken together, the data indicate that the region spanning amino acids 63–72 constitutes a key consensus motif within Erg28p that is required for sterol C-4 demethylation during ergosterol biosynthesis in S. cerevisiae.
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MATERIALS CHEMISTRY AND PHYSICS
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2018
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IF 2.21
Jianwei Fu,Shaomin Wang,Jianhua Zhu,Kai Wang,Meng Gao,Xuzhe Wang,Qun Xu
Highly dispersed Au-Ag bimetallic nanoparticles were facilely synthesized on the multi-amino poly(cyclotriphosphazene-co-polyethyleneimine) (PCP) microspheres through in situ co-reduction method and then used as catalysts for the reduction of 4-nitrophenol (4-NP). Characterization results showed that the as-synthesized PCP microspheres possessed regular spherical morphology and relatively smooth surface, which could be well modified by highly dispersed Au-Ag bimetallic nanoparticles with size of 2–4 nm. For the reduction of 4-NP, it was found that the as-synthesized PCP@Au-Ag composites showed much better catalytic activity than PCP microspheres supported monometallic counterparts, which could be ascribed to a synergistic effect between Au and Ag in Au-Ag bimetals . For the PCP@Au-Ag catalysts, the rate constant k and intrinsic activity parameter κ of the reaction could be up to 2.87 × 10 −3 s −1 and 144 s −1 g −1 , respectively, and the conversion of the 4-NP could still reach 83% after six cycles. In addition, the possible catalytic mechanism of PCP@Au-Ag composites towards the reduction of 4-AP was also proposed.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 6
·1-13
·
IF 5.3
Guo Wenjie,Yu Zhiyong,Li Tianxu,Lu Lingfei,Lin Huiqi,Liao Ying,Zheng Yanghao,Liu Yuntao,Alevtinovna Glaskovich Maria,Barysavets Dzmitry S.,Chen Jinping,Zan Jie,Lu Jiandong
Urine retinol-binding protein 4 (RBP4) has recently been reported as a novel earlier biomarker of chronic kidney disease (CKD) which is a global public health problem with high morbidity and mortality. Accurate and rapid detection of urine RBP4 is essential for early monitor of impaired kidney function and prevention of CKD progression. In the present study, we developed a time-resolved fluorescence immunochromatographic test strip (TRFIS) for the quantitative and rapid detection of urine RBP4. This TRFIS possessed excellent linearity ranging from 0.024 to 12.50 ng/mL for the detection of urine RBP4, and displayed a good linearity ( Y = 239,581 × X + 617,238, R 2 = 0.9902), with the lowest visual detection limit of 0.049 ng/mL. This TRFIS allows for quantitative detection of urine RBP4 within 15 min and shows high specificity. The intra-batch coefficient of variation (CV) and the inter-batch CV were both < 8%, respectively. Additionally, this TRFIS was applied to detect RBP4 in the urine samples from healthy donors and patients with CKD, and the results of TRFIS could efficiently discern the patients with CKD from the healthy donors. The developed TRFIS has the characteristics of high sensitivity, high accuracy, and a wide linear range, and is suitable for rapid and quantitative determination of urine RBP4.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Taotao Zhao,Chenyang Shen,Gui Liu,Jun Yao,Xianghao Wang,Huajie Tao,Yu He,Yuchi Liu,Zhewei Zhang,Feifei Mei,Xiangke Guo,Luming Peng,Yan Zhu,Xuefeng Guo,Nianhua Xue,Weiping Ding
We report here a high-performance catalyst ReMoS x @HSSZ-39 compounded with SAPO-34 for conversion of CO 2 to C 2-4 hydrocarbons, in which the ReMoS x clusters are responsible for highly efficient hydrogenation of CO 2 to methanol as the intermediate and then the surrounding zeolitic acid sites for further conversion of methanol to hydrocarbons. The investigation finds that uniform ReMoS x clusters can be reliably fabricated in zeolitic cages by co-introduction of rhenium and molybdenum species into the HSSZ-39 zeolite and followed by sulfidation, which constitutes an intimate bifunction catalyst through better cooperation of ReMoS x clusters and the zeolitic acid sites. Compared with MoS x @HSSZ-39, the introduction of rhenium significantly suppresses CO generation. By mixing some SAPO-34 zeolite, with increased density of protonic acid, more than 86 % C 2-4 hydrocarbons in organic products and only 21 % CO at 32.5 % of CO 2 conversion are obtained and the composite catalyst shows excellent stability with more than 95 % C 2-4 hydrocarbons in organic products, less than 30 % CO and above 26.6 % of CO 2 conversion after 1000 h on stream.
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CERAMICS INTERNATIONAL
·
2024
·
IF 5.1
Shengqian Wang,Junqiang Gu,Jinying Liu,Feiyan Xie,Chaochao Tao,Shuo Wang,Hualan Xu,Shengliang Zhong
Phosphors with high luminescence efficiency, tunable emission and high color rendering index (CRI) are vital importance for the quality of white light emitting diodes (w-LEDs) in real application. In this work, a new single phase color-tunable Na 5 Lu(WO 4 ) 4 :Tb 3+ ,Eu 3+ phosphor with outstanding luminescent properties was designed and synthesized. Its crystal structure, electronic structure, and luminescence features were studied in detail. The band gap of the host was calculated to be about 4.68 eV. The differential charge distribution after Tb, Eu doping through density functional theory simulation theoretically proved the electron redistribution in the W-O bond upon substitution of Tb 3+ /Eu 3+ . The energy transfer of Tb 3+ →Eu 3+ enabled tunable emission of luminescent colors from green to orange-red due to the quadrupole-quadrupole interaction. The optimal Na 5 Lu(WO 4 ) 4 :60%Tb 3+ ,20%Eu 3+ phosphor exhibited excellent photoluminescence quantum yield of 84.19 % and good luminescent thermal stability (I 423K/298K = 75.90%) with an activation energy of 0.1976 eV. Finally, a white LED device with color coordinates of (0.3371, 0.3636), color rendering index of 89.4 and correlated color temperature of 5334 K was fabricated. These findings demonstrated that Na 5 Lu(WO 4 ) 4 :60%Tb 3+ ,20%Eu 3+ phosphor could be a potential orange-red-emitting color converter for white LEDs. This work not only assisted in the understanding of experimental observations but also provided a theoretical basis for the rational design of phosphors.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Zhaoyan Deng,Yuhan Chen,Qiang Tian,Tianyi Guo,Yanjuan Zhang,Zuqiang Huang,Huayu Hu,Tao Gan
Modulation of the electronic structure of heterogeneous catalysts by introducing heterojunctions is an effective means to improve their catalytic performance. Although Co-based nanocatalysts have been widely used in the reduction of 4-nitrophenol (4-NP) wastewater, Co single-atom catalysts (SACs) have rarely been reported. Herein, we successfully fabricate Co SACs supported on CNT/MoS 2 heterojunction nanocomposite (Co 1 -MoS 2 /CNT) by a hydrothermal synthesis combined with the subsequent impregnation method. The catalytic performance of Co 1 -MoS 2 /CNT is evaluated using the reduction of 4-NP in an aqueous media containing sodium borohydride. Complete reduction of 4-NP catalyzed by Co1-MoS 2 /CNT occurs within 5 min at room temperature, and the apparent rate constant k can reach 1.16 min −1 , which is 4.1 and 38.6 times higher than those of Co 1 -CNT and Co 1 -MoS 2 , respectively. The improved catalytic performance of Co 1 -MoS 2 /CNT is attributed to the p-n heterojunctions of MoS 2 /CNT increasing the electron density of the Co single-atom sites, as well as the CNTs with excellent conductivity, accelerating the electron transfer. Moreover, Co 1 -MoS 2 /CNT exhibits excellent stability and favorable applicability, endowing its potential for practical applications. Our findings not only provide useful insights into the enhancement of catalytic performance of SACs, but also open a new avenue for the treatment of 4-NP wastewater.
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