查找使用阿拉丁产品的科研文献

Hydrophobic charge-induction resin with 5-aminobenzimidazol as the functional ligand: preparation, protein adsorption and immunoglobulin G purification

Jun Yan,Qi-Lei Zhang,Hong-Fei Tong,Dong-Qiang Lin,Shan-Jing Yao

A new hydrophobic charge-induction chromatography resin was prepared with 5-aminobenzimidazol as functional ligand and polyacrylic ester beads as matrix. Adsorption isotherms and adsorption in columns were investigated using human immunoglobulin G and bovine serum albumin as model proteins, and the influence of pH and NaCl concentration was discussed. Results showed that the ligand density was 195 μmol/mL gel, and protein selectivity can be improved by controlling pH and salt addition. An optimized purification process (sample loading at pH 8.0 with 0.2 M NaCl and elution at pH 5.0) was performed to purify human immunoglobulin G from bovine serum albumin containing feedstock, which resulted in human immunoglobulin G purity of 99.7% and recovery of 94.6%. A similar process was applied for the purification of monoclonal antibody from cell culture supernatant, which showed antibody purity of 94.9% and recovery of 92.5%. The results indicated that the new resin developed had comparable performance as Protein A chromatography and would be suitable for antibody purification from complex feedstock.

Recyclable Magnetic Fe3O4-Supported Copper Oxide as Efficient Catalyst for Oxidation of 5-Hydroxymethylfurfural to 2,5-Furanediformic Acid

Peng Yang,Hualei Hu,Yong Yang,Guojun Lan,Soliman I. El-Hout,Qianquan Fang,Guowen Lu,Chunlin Chen,Jian Zhang

The nano-metal oxide-catalyzed oxidation of 5-hydroxymethylfurfural (HMF) with sodium hypochlorite (NaClO) is an effective and mild technique for synthesizing 2,5-furanediformic acid (FDCA). However, the rapid and large-scale separation of nanocatalysts remains a major challenge. In this study, we developed a magnetic and recyclable copper oxide-based catalyst supported on the mechanochemically synthesized Fe3O4. Benefiting from the introduction of the Fe3O4carrier and cetyltrimethylammonium bromide (CTAB) surfactant, the CuO-CTAB/Fe3O4catalyst owns smaller CuO particle sizes, which endows it with excellent catalytic activity in the oxidation of HMF, achieving a high FDCA yield of 90.3%. Furthermore, the rapid magnetic separation of the catalyst effectively inhibits the excessive conversion of FDCA. The recovered catalyst can be reused for at least five cycles without significant loss of catalytic performance, confirming the promising application prospect of the CuO-CTAB/Fe3O4catalyst.

Inhibition of p38 MAPK increases the sensitivity of 5‑fluorouracil‑resistant SW480 human colon cancer cells to noscapine

Zheng Han,Liu Meng,Xiaodong Huang,Jie Tan,Weijie Liu,Wei Chen,Yanli Zou,Yishan Cai,Shasha Huang,Aifang Chen,Ting Zhan,Min Huang,Xiaoli Chen,Xia Tian,Qingxi Zhu

A major cause of treatment failure in advanced colon cancer is resistance to chemotherapy. p38 mitogen‑activated protein kinase (MAPK) has been associated with cellular apoptosis and plays an important role in multidrug resistance (MDR) in cancer cells. In the present study the effect of p38 MAPK on the sensitivity of 5‑fluorouracil (5‑FU)‑resistant SW480 (SW480/5‑FU) human colon cancer cells to noscapine was investigated. Following p38 MAPK interference, the inhibitory effect of noscapine on cell viability and proliferation was increased in the SW480/5‑FU cells and there was also a decrease in the expression level of minichromosome maintenance proteins, recombinant Ki‑67 and proliferating cell nuclear antigen. Inhibition of p38 MAPK also enhanced noscapine‑induced G1‑phase cell cycle arrest in the SW480/5‑FU cells and there was also a decrease in the protein and mRNA expression level of cyclin D, cyclin E and cyclin‑dependent kinase 2, and an increase in the expression level of P57. Furthermore, p38 MAPK interference increased noscapine‑induced apoptosis of the SW480/5‑FU cells and there was an increase in the protein and mRNA expression level of caspases‑3 and 8 and Bax, and decreased Bcl‑2 expression level. The sensitivity of the SW480/5‑FU cells to noscapine was also increased following p38 MAPK interference, as demonstrated by MDR inhibition via decreased Akt activity and reduced protein expression level of the MDR proteins P‑glycoprotein, multidrug resistance protein 1 and ATP‑binding cassette G2. These observations indicated that inhibition of p38 MAPK increased the sensitivity of the SW480/5‑FU cells to noscapine by suppressing proliferation, induction of cell cycle arrest and apoptosis, and reversal of MDR in the SW480/5‑FU cells.

Toluene adsorption on a novel hydrophobic ZSM-5(300)@LDH: Material synthesis, adsorption performances and moisture-resistant characteristics

Jingkai Hu,Huaxin Lin,Zhuowei Cheng,Jianmeng Chen,Ruiqi Zhu,Dongzhi Chen,Zhaoyang Lu

Volatile organic compounds (VOCs), particularly toluene, pose significant environmental and health challenges. This study addresses the limitations of ZSM-5 zeolite in humid environments by integrating Si/Al ratio optimization with hydrophobic modification via layered double hydroxide (LDH) coatings. H-ZSM-5 zeolites with Si/Al ratios of 25, 85, and 300 were synthesized using hydrothermal methods, revealing that higher Si/Al ratios enhance specific surface area (387.67 m 2 /g for Si/Al = 300) and mesopore volume (66.4 %). However, humidity reduced the adsorption capacity (e.g., ZSM-5(300) decreased from 85.37 ± 3.60 to 65.76 ± 2.26 mg/g at 80 % RH). To mitigate moisture interference, three LDH-coated composites were fabricated: self-assembled (ZZ-300@LDH), co-precipitated (GC-300@LDH), and sol-gel-derived (RN-300@LDH). Among these, GC-300@LDH exhibited superior hydrophobicity (water contact angle: 53°) and a hierarchical pore structure (mesopore volume: 0.3688 cm 3 /g), achieving a toluene adsorption capacity of 94.43 ± 1.43 mg/g under 80 % RH—43.6 % higher than ZSM-5(300). The dual adsorption sites and enhanced surface roughness contribute to prolonged breakthrough time (6.6 ± 0.3 min) and stable regeneration over five cycles. Comparative analysis with industrial zeolites highlights GC-300@LDH's potential for integration into zeolite rotor-RTO systems, offering a scalable solution for humid industrial VOC treatment.

3D printing silk-gelatin-propanediol scaffold with enhanced osteogenesis properties through p-Smad1/5/8 activated Runx2 pathway

Caiyun Liu,Zhenzu Bai,Jiayu Lin,Kai Jiang,Shan Huang,Weijia Zheng,Ruifen Chen,Yang Xiang,Xiaoyan Wang,Long Liu

The application of 3 D printing technology in tissue engineering has become increasingly important. However, due to the limitations of bio-ink, there are still some remaining problems. For example, the major challenge for ideal bio-ink is to maintain stable 3 D structure and good biocompatibility in the meantime while conventional gels are week and nearly unprintable. So, the development of new bio-ink material with improved rheological and mechanical properties is highly demanded to avoid compromising biocompatibility for tissue engineering. Silk fibroin (SF), a natural degradable polymer, is considered to be a proper material for the preparation of bio-inks. We used SF, gelatin, and polyols as raw materials to fabricate bio-inks and scaffolds. We evaluated the rheological properties and printability of bio-inks with a rotational rheometer and a 3 D printer. The scaffolds were prepared by crosslinking and freeze-drying technologies. The biocompatibility and osteoinductive functions of scaffolds were investigated by evaluating proliferation, osteogenic differentiation and related cell signaling of cultured MC3T3-E1 cells. The results showed that the scaffolds using SF, Gel and propanediol (PG) not only had good rheological properties and storage modulus, but also could better enhance osteogenic specific genes expression mediated by Smad1/5/8 and Runx2 pathways. What is more, morphological characterization showed that α-mem incubation could help scaffold form porous structure on its surface, which could shed a light on a new 3 D bio-printed bone repair scaffold with both naturally emerged and CAD-designed porous structure. Our findings provide a potential biomaterial for the treatment of bone tissue regeneration.

A mutually beneficial waste-to-biochar strategy: Mn-mediated dual enhancement of 5-HMF production and catalytic performance

Yan Sun,Ranxin Li,Qiubo Wang,Wen-da Oh,Mingjie Huang,Xiaohui Wu,Tao Zhou

The concept of converting biowaste to energy is popular worldwide; however, overcoming the trade-off between bio-oil recovery and obtaining functional biochar remains challenging. This study investigated the effects of three transition metal ions (Mn 2+ , Cu 2+ and Fe 2+ ) throughout the entire process of recovering 5-hydroxymethylfurfural (5-HMF) from forestry waste and on the catalytic abilities of biochar derived from the oil-extracted residue. The Cu 2+ , Fe 2+ , and Mn 2+ ions significantly increased 5-HMF yields by 93- to 158-fold compared to the metal-free control. However, only marginal improvements in the catalytic ability of the residue-derived biochar for persulfate activation were observed for Cu- or Fe-doped biochar, despite the general benefit of transition metal doping for biochar functionalization. Interestingly, a dual Mn-mediated promotion was achieved beyond enhancing 5-HMF production. The Mn-doped biochar (Mn-Biochar) exhibited excellent peroxydisulfate (PDS) activation capability, enhancing the degradation of the antibiotic sulfamethoxazole (SMX) by 6.6- to 15.0-fold. Characterizations revealed that incorporated nitrogen was responsible for this enhancement. N-doping transformed the Mn species into MnO, which tripled the Mn loading capacity and enhanced the electrical conductivity of the biochar. The formed Mn-N x structure in the biochar (Mn-N-FBBC) facilitated polarization of the O O bond and cleavage of the S O bond in PDS, generating 1 O 2 for SMX degradation. Life cycle assessment (LCA) indicated that this dual Mn-mediated process for 5-HMF recovery and biochar production offers synergistic benefits, realizing the “waste-resource-material-treatment” paradigm in biomass conversion.

Inhibitory effect of protonic bis(5-amino-1,10-phenanthroline) on proliferation of hepatocellular carcinoma and its molecular mechanism

Zizhen Zhao,Chen Fu,Yuping Zhang,Yingying Zhang,Xiaoxi Yang,Ailing Fu

The incidence of HCC continues to increase rapidly in recent years. the principle of developing anti-HCC drugs is to specifically kill tumor cells, without affecting the function of normal cells. Here we synthesized a novel protonic compound bis(5-amino-1,10-phenanthroline) (P-BAP), and the antitumor activity was explored in vitro and in vivo . The results showed that P-BAP could selectively inhibit the proliferation of tumor cells, and induce HCC apoptosis. In HCC-bearing mice, P-BAP could effectively retard the tumor growth, even completely eliminate the tumors at the dose of 5 mg/kg, and meanwhile P-BAP had no significant effect on mouse body weight. Mechanism analysis revealed that P-BAP could down-regulate the protein expressions of pleomorphic adenoma gene like-2 (PLAGL2), HIF-1α and β-catenin, and up-regulate the levels of pro-apoptotic protein BAX and BNIP3. In addition, P-BAP could reduce mitochondrial respiratory chain complex activities, leading to insufficient ATP production. The study provides a new approach for designing selective antitumor drugs, and suggests that P-BAP would be a potential candidate for HCC therapy.

Construction of Hierarchical High Acid-Capacity ZSM-5 for Enhancing Mass Transfer and Aromatization of Lignite Pyrolysis Volatiles

Nai-Yu Yao,Jing-Pei Cao,Shi-Jie Cai,Chen-Xu Chen,Xiao-Yan Zhao,Xin-Bo Pang,Min Xu,You Lu,Xiao-Bo Feng,Tungalagtamir Bold

ZSM-5 is an effective catalyst for the catalytic reforming of volatiles from lignite. An increased density of acidic sites significantly enhances catalytic activity. However, its inherent microporous structure and abundant external surface acids lead to issues including catalyst deactivation. In this work, a nondiscriminatory etching method using NH4F was employed to simultaneously remove Si and Al species from highly acidic zeolites. This process introduces mesopores while reducing external surface acidity, maintains high crystallinity, and preserves the total acid content essentially unchanged. The mesoporous surface area of HZ5-6.5 reaches 129 m2/g. This significantly enhances the mass transfer efficiency of volatile component molecules within the zeolite, effectively improving the accessibility of active sites. Furthermore, compared to the parent zeolite, the modified HZ5-6.5 exhibits a 50% reduction in acidic sites on its crystal surface while maintaining essentially unchanged total acidity. Concurrently, light aromatic yields increase, while coke deposition decreases by 40%.

Preparation and performance evaluation of asphalt modifier from catalytic pyrolysis of waste plastics using Ni modified HZSM-5

Juhao Wu,Hong Tian,Fan Gu,Hai Ning,Junjie Huang,Binbin Chen,Yanlin Zeng,Huimin Mao,Xinmiao Wang

Pyrolysis technique can effectively convert waste plastics into high-performance asphalt modifiers, improving the asphalt pavement performance, thus achieving the resourceful utilization of waste plastics as building materials. Selecting Polypropylene (PP) and polystyrene (PS) as raw materials, pyrolysis experiments were conducted to explore their synergistic interaction. Using Thermogravimetric analysis (TGA), tube furnace, and gas chromatography-mass spectrometry (GCMS), the effects of plastics mixing ratio and Ni loading in HZSM-5 on the pyrolysis products and heavy oil composition were investigated. Based on the optimal heavy oil, the high and low temperature performance and aging resistance of the heavy oil modified asphalt were quantitatively evaluated. The results showed that the PP/PS co-pyrolysis exhibited higher conversion efficiency, with a 1:3 ratio maximizing liquid yield at 74.26 %. Ni-modified HZSM-5 effectively boosted the production of aromatic hydrocarbons in heavy oil while inhibiting the generation of phenols, acids, organosilicons and other non-hydrocarbon byproducts. The optimal Ni loading was 10 wt%, which maximized aromatic hydrocarbon content at 81.36 % within 90.6 % polycyclic aromatic hydrocarbons (PAHs), achieving the best modulation of pyrolysis heavy oil components. The addition of heavy oil significantly improved the high-temperature stability and aging resistance of asphalt, though impairing low-temperature cracking resistance. The optimal oil content was 6 wt%, making the softening point increased by 10.3°C. This advancement promoted the conversion of waste plastic heavy oil into high-performance asphalt modifiers to avoid difficult combustion, providing theoretical support and technical guidance for its high-value application in the asphalt pavement field.

Formulation of pH-sensitive ZIF-8 (MOF) impregnated amino-chitosan biocomposite beads for sustained release of 5-fluorouracil

Ahmed M. Omer,Eman M. Abd El-Monaem,Heba S. Hassan,Mahmoud A. Elmeligy,Angela Kleinová,Abdelazeem S. Eltaweil,Abolfazl Heydari

pH-sensitive carriers have emerged as an optimal choice for the oral administration of anticancer drugs, exhibiting the desired release performance. This study aims to develop biocomposite beads for the sustained release of the anticancer drug 5-fluorouracil (5-FU). Here, 5-FU was encapsulated within a ZIF-8 metal-organic framework (MOF), which was subsequently impregnated into amino-chitosan beads. The resulting Am-CS@ZIF-8 biocomposite beads were characterized for their chemical structure, surface morphology, elemental composition, crystallinity, surface charges, and mechanical properties utilizing Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), zeta potential analysis, and a texture analyzer, respectively. The developed beads exhibited a textured surface, denser composition, and a positively charged surface with a maximum zeta potential of +12.4 at pH 7.4. In comparison to Am-CS beads, the Am-CS@ZIF-8 biocomposite beads demonstrated superior mechanical properties, achieving a maximum stress value of 5.668 MPa compared to the 1.874 MPa recorded for pure Am-CS beads. pH sensitivity was assessed through the investigation of water uptake behavior at pH 1.2 and pH 7.4. The Am-CS@ZIF-8 biocomposite beads demonstrated a high encapsulation efficiency of approximately 81.6 % for 5-FU. They exhibited pH-responsive release behavior, with only 17 % of the drug released at pH 1.2 after 2 h and a sustained release of 52 % at pH 7.4 over 24 h. The release mechanism was predominantly governed by Fickian diffusion, as closely fitted by the Peppas-Sahlin kinetic model. The formulated carrier confirmed its biodegradability under enzymatic physiological conditions, suggesting its potential applicability as an effective system for the oral delivery of anticancer drugs.

Research on the Effect of 5-Aminoindole on Polymerization Behaviors and Mechanical Properties of Poly(Aldehyde Functional Benzoxazine)

Yi Xu,Zhengbai Li,Nan Yang,Yujia Liu,Qiang He,Wenfeng Yang,Chao Li

5-ALA, DTA-6, and Nitrogen Mitigate NaCl Stress by Promoting Photosynthesis and Carbon Metabolism in Rice Seedlings

Yaxin Wang,Chaolu Tan,Yinghao Li,Fengyan Meng,Youwei Du,Shuyu Zhang,Wenxin Jiang,Naijie Feng,Liming Zhao,Dianfeng Zheng

A large number of dead seedlings can occur in saline soils, which seriously affects the large-scale cultivation of rice. This study investigated the effects of plant growth regulators (PGRs) and nitrogen application on seedling growth and salt tolerance (Oryza sativaL.), which is of great significance for agricultural production practices. A conventional rice variety, “Huang Huazhan”, was selected for this study. Non-salt stress treatments included 0% NaCl (CK treatment), CK + 0.05 g N/pot (N treatment), CK + 40 mg·L−15-aminolevulinic acid (5-ALA) (A treatment), and CK + 30 mg·L−1diethylaminoethyl acetate (DTA-6) (D treatment). Salt stress treatments included 0.3% NaCl (S treatment), N + 0.3% NaCl (NS treatment), A + 0.3% NaCl (AS treatment), and D + 0.3% NaCl (DS treatment). When 3 leaves and 1 heart emerged from the soil, plants were sprayed with DTA-6 and 5-ALA, followed by the application of 0.3% NaCl (w/w) to the soil after 24 h. Seedling morphology and photosynthetic indices, as well as carbohydrate metabolism and key enzyme activities, were determined for each treatment. Our results showed that N, A, and D treatments promoted seedling growth, photosynthesis, carbohydrate levels, and the activities of key enzymes involved in carbon metabolism when compared to the CK treatment. The A treatment had the most significant effect, with increases in aboveground dry weight and net photosynthetic rates (Pn) ranging from 17.74% to 41.02% and 3.61% to 32.60%, respectively. Stomatal limiting values (Ls) significantly decreased from 19.17% to 43.02%. Salt stress significantly inhibited seedling growth. NS, AS, and DS treatments alleviated the morphological and physiological damage of salt stress on seedlings when compared to the S treatment. The AS treatment was the most effective in improving seedling morphology, promoting photosynthesis, increasing carbohydrate levels, and key enzyme activities. After AS treatment, increases in aboveground dry weight, net photosynthetic rate, soluble sugar content, total sucrose synthase, and amylase activities were 17.50% to 50.79%, 11.39% to 98.10%, 20.20% to 80.85%, 21.21% to 33.53%, and 22.17% to 34.19%, respectively, when compared to the S treatment. In summary, foliar sprays of 5-ALA, DTA-6, and additional nitrogen fertilizer enhanced rice seedling growth, increased photosynthesis, lowered Ls values, and improved seedling salt tolerance. Spraying two regulators, 5-ALA and DTA-6, quantitatively increased the effect of nitrogen fertilizer, with comparable effects on NaCl stress regulation. This study provides the basis for efficient agricultural production.