NANOTECHNOLOGY
·
2019
·Vol. 30
·期 35
·355604
·
IF 3.399
Keju Zhang,Dong Zhou,Zhiguo Wang,Yuhong Zhang,Peixin He
The synthesis of drug delivery systems based on surface-modified mesoporous silica hollow structures remains a huge challenge. In this paper, we have obtained hollow mesoporous silica nanoparticles (MSNs) by surfactant directed sol-gel assisted hydrothermal treatment. The MSNs have the inorganic-organic hybrid frameworks with uniform diameter distribution (260 nm), and their specific surface area, mesoporous size and pore volume are 540 m2 g−1, 3.7 nm, 0.58 cm3 g−1, respectively. It was proved that the preparation of hollow ethane-bridged nanospheres with two silicon source was due to the high crosslinking of the silicone interface and hydrothermal treatment, providing a new approach for the study of drug-loaded and controlled release behavior. Based on the synthesis of MSNs, MSNs were modified by methacryloxy propyl trimethoxyl silane (MPS) on the surface of MSNs. Then N-isopropylacryamide (NIPAM) and acrylic acid (AA) were grafted onto the surface of modified MSNs. The hollow ethane-bridged PNA–MSNs (poly (NIPAM-co-acrylic acid)-MSNs) with two silicon source were prepared successfully. Due to their distinctive hollow structure, PNA–MSNs demonstrated high drug encapsulation efficiency (70.4% ± 2.9%). The experiment results proved that the modified hollow nanoparticles not only had good biocompatibility and stability, but also possessed pH-/thermal-dual responsiveness in drug release.
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Analytical Methods
·
2019
·Vol. 11
·期 20
·2663-2668
·
IF 2.378
Dan Zhu,Shujuan Zhuo,Changqing Zhu,Ping Zhang,Weili Shen
In this study, we have presented a novel strategy to fabricate fluorescent Fe-doped carbon dots (Fe-CDs) by one-step hydrothermal carbonization, using methylthymol blue sodium salt (MTB) and FeCl3·6H2O as precursors. Multiple techniques were employed to characterize the properties of the obtained Fe-CDs. Importantly, the intrinsic peroxidase-like activity of the Fe-CDs was explored. Through catalyzing the oxidization of o-phenylenediamine (OPD) by hydrogen peroxide (H2O2), the Fe-CDs exhibited a superior catalytic performance over horseradish peroxidase (HRP) attributed to the doped Fe elements in the Fe-CDs. By the combination of favorable fluorescence properties and excellent catalytic activities, Fe-CD based ratiometric fluorescence sensing systems toward H2O2 and glucose have been designed in view of the inner filter effect (IFE) between Fe-CDs and 2,3-diaminophenazine (DAP, the oxidation product of OPD). Such responses can be used for well quantifying H2O2 and glucose in the ranges of 0–133 μM and 0–300 μM with detection limits of 0.47 and 2.5 μM (S/N = 3), respectively. The proposed sensing system has been successfully used for the assay of glucose in human serum samples, indicating great promise as a practical platform for blood glucose sensing.
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ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Jingmin Wang,Xiaolong Hu,Haizhen Ding,Xin Huang,Mingsheng Xu,Zhenzhen Li,Dong Wang,Xu Yan,Yang Lu,Yunjun Xu,Yan Chen,Paulo Cesar Morais,Yupeng Tian,Ren-Quan Zhang,Hong Bi
Commercial gadolinium-based materials have been widely used as contrast agents for magnetic resonance imaging (MRI), but the high toxicity of leaking free Gd3+ ions still raises biosafety concerns. Here, we develop a novel, safe, and efficient MRI contrast agent based on a stable Fe(III) complex of fluorine and nitrogen co-doped carbon dots (F,N-CDs) that was prepared from glucose and levofloxacin by a simple microwave-assisted thermal decomposition method. The obtained Fe3+@F,N-CD complex exhibits higher longitudinal relaxivity (r1 = 5.79 mM–1·s–1) than that of the control samples of the Fe3+@CD complex (r1 = 4.23 mM–1 s–1) and free Fe3+ (r1 = 1.59 mM–1 s–1) in aqueous solution, as assessed by a 1.5 T NMR analyzer. More importantly, the Fe3+@F,N-CD complex is very stable with a large coordination constant of 1.06 × 107 in aqueous medium. While incubated with HeLa cells, the Fe3+@F,N-CD complex shows clear MR images, demonstrating that it has potential to be an excellent MRI contrast agent. Furthermore, in vivo MRI experiments indicate that the Fe3+@F,N-CD complex provides high-resolution MRI pictures of 4T1 tumor bearing BALB/c mice 15 min after injection and can be completely excreted 2 h after administration. No cytotoxicity was observed with F,N-CDs and Fe concentration up to 0.2 mg/mL and 0.3 mM in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cell proliferation assay, respectively. The possible mechanism of the enhanced MRI effect of the Fe3+@F,N-CD complex is therefore proposed. The extremely low toxicity, high r1 relaxivity, strong photoluminescence, and low synthetic cost enable the Fe3+@F,N-CD complex to be a safe and promising T1-weighted MRI contrast agent for clinical applications.
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ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Ding Wang,Yaqin Chai,Yali Yuan,Ruo Yuan
In this work, a rigid DNA tetrahedron (TDN) scaffold was synthesized to precisely control the interenzyme distance by randomly anchoring two pairs of horseradish peroxidase (HRP)/glucose oxidase (GOx) at the vertices of TDN, which could not only avoid the drawbacks of poor controllability and biocompatibility from traditional scleroid skeletons, but also overcome the defect of imprecise regulation for interenzyme distance caused by DNA origami. Impressively, by varying the side length of TDN scaffold, the interenzyme distance was precisely regulated, thus, an optimal TDN scaffold with highest catalytic efficiency was acquired and subsequently applied for constructing an ultrasensitive biosensor for DNA detection with a low detection limit of 3 fM. This strategy paved an avenue for developing new reliable scaffold to precisely regulate the catalytic efficiency of enzyme cascade reaction with ultimate applications in bioanalysis and clinical diagnosis.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 9
·1-8
·
IF 5.479
Peng Yage,Dong Wenfei,Wan Le,Quan Xiaosai
Molybdenum disulfide quantum dots (MoS 2 QDs) are used in a fluorometric method for the determination of folic acid (FA) based on fluorescence quenching. The MoS 2 QDs synthesized by a hydrothermal method possess bright blue fluorescence (with excitation/emission maxima of 325/415 nm), quantum yield of 3.7%, and excellent storage stability in solution (30 days in the refrigerator). Their fluorescence is quenched by FA, and intensity decreases linearly in the 0.1 to 125 μM FA concentration range. The detection limit is 0.1 μM (at S/ N = 3), and the relative standard deviation (for n = 5) is 2.8% for 25 μM concentrations of FA. Studies on the quenching mechanism suggest that the effect is due to static quenching. The FA in commercial FA tablets was successfully determined.
Schematic representation of the hydrothermal method for the preparation of molybdenum disulfide quantum dots (MoS 2 QDs) with about 2.7 ± 0.5 nm diameter using Na 2 MoO 4 and L-cysteine as Mo and S sources, and the fluorescence method for the determination of folic acid (FA) based on fluorescence quenching of MoS 2 QDs.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 8
·1-8
·
IF 5.479
Li Yuanyuan,Du Qianqian,Zhang Xiaodan,Cao Haiyan,Huang Yuming
The authors describe the preparation of gold nanoclusters (AuNCs) capped with kojic acid. The capped AuNCs exhibit bright green fluorescence (peaking at 500 nm upon excitation at 375 nm), a nanosecond lifetime (0.37 ns), and a quantum yield (QY) of 22% in aqueous solution. This is higher than most of the previously reported AuNCs. The QY increases to 58% due to aggregation-induced emission in ethanol solution, and the lifetime is prolonged to 1.3 ns. The fluorescence of the KA-AuNCs is quenched by Eu(III) ion but is recovered by addition of phosphate due to its stronger affinity for Eu(III). Under the catalytic action of alkaline phosphatase (ALP), ascorbic acid phosphate (AAP) is transformed to free phosphate. On this basis, a fluorogenic assay for ALP was established. Response is linear in the 0.2 to 20 U·L −1 activity range, and the detection limit is 0.04 U·L −1 (at S/ N = 3). The assay was successfully applied to the determination of the activity of ALP in spiked human serum and also to screen for its inhibitors.
Highly luminescent and stable gold nanoclusters (AuNCs) with aggregation-induced emission property were synthesized through non-thiolate ligand kojic acid (KA) and demonstrated as an efficient probe for screening for alkaline phosphatase (ALP) activity and its inhibitors.
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DRUG DELIVERY
·
2019
·
IF 3.829
Fangyuan Guo,Qiafan Fu,Chenhao Jin,Xugang Ji,Qinying Yan,Qingliang Yang,Danjun Wu,Ying Gao,Weiyong Hong,Aiqin Li,Gensheng Yang
The limitations of anticancer drugs, including poor tumor targeting and weak uptake efficiency, are important factors affecting tumor therapy. According to characteristics of the tumor microenvironment, in this study, we aimed to synthesize matrix metalloproteinase (MMP)-responsive curcumin (Cur)-loaded nanoparticles (Cur-P-NPs) based on amphiphilic block copolymer (MePEG-peptide-PET-PCL) with MMP-cleavable peptide (GPLGIAGQ) and penetrating peptide (r9), modified to improve tumor targeting and cellular uptake. The average size of Cur-P-NPs was 176.9 nm, with a zeta potential of 8.1 mV, and they showed drug entrapment efficiency and a loading capacity of 87.07% ± 0.63% and 7.44% ± 0.16%, respectively. Furthermore, Cur release from Cur-P-NPs was sustained for 144 h at pH 7.4, and the release rate was accelerated under enzyme reaction condition. The MTT assay demonstrated that free P-NPs had favorable biosafety, and the anti-proliferative activity of Cur-P-NPs was positively correlated with Cur concentration in MCF-7 cells. Additionally, the results of cellular uptake, in vivo pharmacokinetics, and biodistribution showed that Cur-P-NPs had a good effect on cellular uptake and tumor targeting, resulting in the best bioavailability in tumor therapy. Therefore, Cur-P-NPs, as a promising drug delivery system, might lead to a new and efficient route for targeted therapy in clinical practice.
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2019
·
IF 3.973
Kaiyu Wang,Yiteng Lei,Donglin Xia,Peipei Xu,Tao Zhu,Zhongying Jiang,Yuqiang Ma
Natural cellular membranes, with the outstanding qualities of biocompatibility and specificity, have gained growing attentions in the system of drug delivery. Nanoparticles coated with cellular membranes are starting to be applied as drug-loaded-vehicles to target tumors. Here, neutrophil membranes were selected to apply in the treatment of inflammation because neutrophils can participate in various inflammatory responses and accumulate at inflammatory sites to eliminate pathogens. Through extracting neutrophil membranes from natural neutrophils without affecting their biological properties, nanoparticles loaded with sparfloxacin (SPX) were coated with these membranes and disguised as neutrophils. Compared with traditional nano-medicines, the neutrophil membrane-coated nanoparticles (NM-NP-SPX) possessed precise targeting ability just like the neutrophils could accumulate at inflammatory sites when inflammation burst. In addition, NM-NP-SPX could prolong the circulation time and had the property of controlled-release. Through in vivo experiments, we found that the concentration of three representative inflammatory cytokines in blood, bacteria and inflammatory cells in lungs of the mice with pneumonia reduced significantly in the initial 24 h after the injection of NM-NP-SPX, which meant that NM-NP-SPX could greatly reduce the risk of death for the patients with inflammation. Moreover, the infected lungs could recover rapidly without any side effects to other organs due to the low cytotoxicity of NM-NP-SPX against normal cells. Therefore, our developed drug delivery system has enormous advantages in treating inflammations. Not only that, this kind of bionic method may have greater value and application prospects in curing the inflammations arisen from cancers.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2019
·
IF 3.714
Ru-yi Li,Shaoman Wang,David Julian McClements,Yin Wan,Cheng-mei Liu,Gui-ming Fu
Small molecules with beneficial biological activities can be generated by controlled fermentation of natural food products. In this study, the fermentation products of tannic acid by Aspergillus carbonarius FCYN212 were isolated and identified. Two major chemical structures were obtained: gallic acid (GA) and 3,4,6-tri- O -galloyl- d -glucose (TGG). GA and TGG exhibited stronger free radical scavenging ability and ferric-reducing power than butylated hydroxyanisole (BHA). Additionally, TGG demonstrated significant dose-dependent enzyme inhibitory activities against α-amylase and α-glucosidase with IC 50 values of 334.6 ± 8.9 and 46.5 ± 1.6 μM, respectively. Notably, TGG was a mixed type inhibitor of α-amylase and α-glucosidase according to a Dixon plot. This study revealed the antioxidant and enzyme inhibitory potential of TGG isolated from a fermented extract of tannic acid. TGG may therefore serve as an antioxidant and antidiabetic ingredient in functional or medical foods.
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ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Shen-Zhen Ren,Dan Zhu,Xiao-Hua Zhu,Bin Wang,Yu-Shun Yang,Wen-Xue Sun,Xiao-Ming Wang,Peng-Cheng Lv,Zhong-Chang Wang,Hai-Liang Zhu
Responsive nanocarriers with biocompatibility and precise drug releasing capability have emerged as a prospective candidate for anticancer treatment. However, the challenges imposed by the complicated preparation process and limited loading capacities have seriously impeded the development of novel multifunctional drug delivery systems. Here, we developed a novel and dual-responsive nanocarrier based on a nanoscale ZIF-8 core and an organosilica shell containing disulfide bridges in its frameworks through a facile and efficient strategy. The prepared ZIF-8@DOX@organosilica nanoparticles (ZDOS NPs) exhibited a well-defined structure and excellent doxorubicin (DOX) loading capability (41.2%) with pH and redox dual-sensitive release properties. The degradation of the organosilica shell was observed after 12 h incubation with a 10 mM reducing agent. Confocal imaging and flow cytometry analysis further proved that the nanocarriers can efficiently enter cells and complete intracellular DOX release under the low pH and high glutathione concentrations, which resulted in an enhanced cytotoxicity of DOX for cancer cells. Meanwhile, subcellular localization experiments revealed that the ZDOS NPs entered cells mainly by endocytosis and then escaped from lysosomes into the cytosol. Moreover, in vivo assays also demonstrated that the ZDOS NPs exhibited negligible systemic toxicity and significantly enhanced anticancer efficiencies compared with free DOX. In summary, our prepared pH and redox dual-responsive nanocarriers provide a potential platform for controlled release and cancer treatment.
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Biomaterials Science
·
2018
·Vol. 6
·期 8
·2172-2188
·
IF 5.831
Haifeng Sun,Dinglingge Cao,Yanhong Liu,Hui Wang,Xue Ke,Tianyuan Ci
Tumor metastasis has become a major obstacle for the clinical treatment of malignant breast cancer. Thus, a delivery system capable of both antitumor and anti-metastasis efficacy is desired. In this work, a low molecular weight heparin (LMWH)-based reduction-sensitive delivery system was developed, aiming to combine the properties of both the antitumor drug and active excipients to achieve a synergistic antitumor and anti-metastatic efficiency as well as resolving the potential adverse effects of both doxorubicin and LMWH. That is, the efficiency of DOX could be enhanced and its toxicity could be lowered. Meanwhile, the biological properties of LMWH could be strengthened and its bleeding risk could also be alleviated. Briefly, drug-loaded crosslinked nanoparticles (DOX/cLLHC2) had a longer blood circulation time and exhibited a rapid reduction-triggered release of DOX. The in vitro assays revealed that DOX/cLLHC2 exhibited a higher cellular uptake and cytotoxicity compared to free DOX. Meanwhile, for the existence of LMWH, DOX/cLLHC2 inhibited cell migration and invasion, as well as effectively inhibited the tube-like formation of human umbilical vein endothelial cells. DOX/cLLHC2 possessed a superior tumor accumulation compared to lipoic acid unmodified nanoparticles and free DOX. Also, DOX/cLLHC2 exhibited encouraging antitumor and anti-metastasis efficiency in an orthotopic breast cancer model. Overall, DOX/cLLHC2 could exert antitumor, anti-metastasis, and anti-angiogenesis efficacy simultaneously as well as having lower systemic toxicity, which makes it suitable for the therapy of metastatic breast carcinoma.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2018
·Vol. 410
·期 20
·4805-4813
·
IF 3.307
Wang Yanying,Liu Yaqin,Ding Fang,Zhu Xiaoyan,Yang Li,Zou Ping,Rao Hanbing,Zhao Qingbiao,Wang Xianxiang
In this study, we developed a simple colorimetric approach to detect glutathione (GSH). The proposed approach is based on the ability of CuS-PDA-Au composite material to catalytically oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to ox-TMB to induce a blue color with an absorption peak centered at 652 nm. However, the introduction of GSH can result in a decrease in oxidized TMB; similarly, it can combine with Au nanoparticles (Au NPs) on the surface of CuS-PDA-Au composite material. Both approaches can result in a fading blue color and a reduction of the absorbance at 652 nm. Based on this above, we proposed a technique to detect GSH quantitatively and qualitatively through UV-Vis spectroscopy and naked eye, respectively. This approach demonstrates a low detection limit of 0.42 μM with a broad detection range of 5 × 10 −7 –1 × 10 −4 M with the assistance of UV-Vis spectroscopy. More importantly, this approach is convenient and rapid. This method was successfully applied to GSH detection in human serum and cell lines.
A colorimetric approach has been developed by exploiting the peroxidase-like activity of CuS-polydopamine-Au composite for sensitive glutathione detection.
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危险品化学品经营许可证（带存储）