MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 12
·1-11
·
IF 6.408
Wang Yue,Zhai Hongguo,Yin Jiaqi,Guo Qi,Zhang Yuhao,Yang Qingqing,Li Falan,Sun Xia,Guo Yemin,Zhang Yanyan
With the development of exonuclease, the exonuclease has been used to construct a variety of aptasensor and to realize the signal amplification. Among them, based on silver nanoparticles (Ag NPs) and exonuclease I (Exo I)-assisted cycle signal amplification strategy, we designed a novel high-sensitivity dual-target electrochemical biosensor to detect Pb 2+ or Hg 2+ in water. In the presence of Hg 2+ , the Hg 2+ was fixed to the aptamer chain by thymine-Hg 2+ -thymine (T-Hg 2+ -T), resulting in the decrease of signal. When Pb 2+ was present, DNA single strand S2 dissociated and was bound to Pb 2+ , which automatically triggered Exo I to selectively cut the single chain from the recognition site to achieve the cyclic amplification of the electrochemical signal. The interaction between aptamer and Exo I was investigated by gel electrophoresis. Under the optimum conditions in the scan range -0.20 to 0.60 V, the biosensor had high sensitivity with a linear range of 100 pg/L to 10.0 mg/L, Pb 2+ or Hg 2+ , and the detection limits were 17.0 pg/L ( R 2 = 0.993) and 12.0 pg/L ( R 2 = 0.993), respectively. The relative standard deviation (RSD) of the sensor was 0.5–2.6%, and the recovery of spiked standard solutions was between 98.3 and 110%. The cycle amplification strategy supported by this enzyme has promising applications in detection of the two metal ions in various fields. Graphical Abstract
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SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Chao Jiang,Yansheng Li,Hui Wang,Desheng Chen,Yongqiang Wen
We proposed a facile DNA-based hydrogel capillary sensor (DHCS) for sensitive detection of Pb 2+ . This proposed biosensor took advantage of the capillary action of the capillary tube controlled by the hydrogel film and the mesh size of the hydrogel based on target-responsive DNA. In the presence of Pb 2+ , the crosslinker substrate strand was cleaved leading the hydrogel fractured partly, and the mesh size of the hydrogel film plugging the end of the capillary tube changed. Owing to this, the behavior of the solution flowing through the capillary controlled by the hydrogel film was affected by the concentration of Pb 2+ . Therefore, through reading the distance and duration time by the naked eye, quantitative detection of Pb 2+ was achieved without any equipment. Additionally, and importantly, the tiny Pb 2+ responsive DNA hydrogel (0.02 μL) can be directly applied to analysis without encapsulation operations of nanoparticles or enzymes. Benefiting from the DNAzyme merits of high sensitivity and selectivity, in the range of 0.01 –50 μM, the developed method was able to detect as low as 10 nM Pb 2+ in less 1 h with the distance under 20 mm, meanwhile, the signals of blank and other high concentration metal ions were negligible. This sensor could be utilized to detect Pb 2+ in tap water with good reliability. Therefore, this proposed strategy provided a miniature, portable, sensitive and highly selective, visual platform for the detection of Pb 2+ in situ measurement.
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FOOD CHEMISTRY
·
2021
·
IF 7.514
Shuang Chen,Yujie Liu,Fei Zhai,Min Jia
A novel label-free fluorescence aptasensor was established for chloramphenicol (CAP) detection by DNA four-arm junction-assisted target recycling and SYBR Green I dye-aided fluorescence-signal amplification. The CAP aptamer was hybridized to its complementary strand (primer) to form a double-stranded primer/aptamer complex. In the presence of CAP, aptamers can specifically bind with CAP to dissociate primers, which can trigger the self-assembly of four hairpins to continuously generate DNA four-arm junctions. After digesting the excess hairpins using T7 exonuclease, SYBR Green I was inserted into the base pair-rich DNA four-arm junctions, which led to a significant increase in fluorescence intensity. Under optimal conditions, the developed aptasensor can detect CAP in a linear range of 1.0 pg mL −1 to 10 ng mL −1 with a detection limit of 0.72 pg mL −1 . The recovery rates in milk and honey ranged from 90.3% to 106.6%. Thus, the method shows substantial potential for CAP detection in food products.
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INORGANICA CHIMICA ACTA
·
2022
·
IF 3.118
Xin Yan,Jia-Qian Chen,Mao-Lin Hu,Hiroshi Sakiyama,Mohd. Muddassir,Jian-Qiang Liu
Two different silver(I) complexes with diphenylphosphonic acid as peripheral ligand were designed and synthesized by using different solvents. The complexes were characterized by X-ray photoelectron spectroscopy (XPS), infrared spectroscopy (IR), elemental analysis (EA), thermogravimetric analysis (TG). Single crystal X-ray analysis established the final structure of both complexes. The interaction modes of the two clusters with DNA were investigated by fluorescence and electrochemical analysis. The theoretical result was justified with the help of theoretical data. The results established that the cluster with methanol solvent was more stable binding with the DNA and protein than the cluster with DMF molecule.
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MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 4
·1-9
·
IF 6.232
Cao Xiaodong,Zhang Kairui,Yan Wuwen,Xia Zihao,He Shudong,Xu Xuan,Ye Yongkang,Wei Zhaojun,Liu Songqin
A selective and sensitive fluorescence biosensor is described for determination of microRNA-167 using fluorescent resonant energy transfer (FRET) strategy. The FRET system comprises carbon dots (CDs, donor) labeled with probe DNA (pDNA) and polydopamine (PDA)-coated Fe 3 O 4 nanoparticles (Fe 3 O 4 @PDA NPs, acceptor). The CDs-pDNA can be absorbed onto the surface of Fe 3 O 4 @PDA NPs because of the strong π interaction between pDNA and PDA. With the enhanced adsorption ability of Fe 3 O 4 @PDA NPs by Ca 2+ , the fluorescence intensity of CDs at 445 nm (excitation at 360 nm) is quenched. In presence of microRNA-167, the hybridized complex of CDs-pDNA-microRNA-167 will be released from the surface of Fe 3 O 4 @PDA NPs due to the weak π interaction of the complex and PDA. This results in the fluorescence recovery of CDs. By application of twice-magnetic separation, the biosensor shows a wide linear range of 0.5–100 nM to microRNA-167 with a 76 pM detection limit. The method was applied to the determination of microRNA-167 in samples of total microRNA extractions from A. thaliana seedlings, and the recoveries ranged from 96.4 to 98.3%.
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ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Xunxun Deng,Shuo Wu,Zhipeng Li,Yanqiu Zhao,Chunying Duan
Fluorescence ratiometric biosensors are valuable tools for the accurate and sensitive prediction and diagnosis of diseases. However, seldom have fluorescence ratiometric biosensors for protein and DNA been reported because of the shortage of suitable nanoscale scaffolds. Herein, a tripyridinyl RuII complex–encapsulated SiO2@polydopamine (Ru–SiO2@PDA) nanocomposite was designed as a universal platform for fluorescence ratiometric detection of DNA and protein in serum samples. The Ru–SiO2@PDA nanocomposites have a narrow size distribution, exhibit good biosafety, and are convenient for the postmodification of biorecognition elements. Under irradiation, they can emit a stable and strong luminescence at 650 nm and simultaneously quench the fluorescence emitted from the fluorophores getting close to them. Once the capture probes such as single-stranded DNA and aptamer are assembled, the fluorophores labeled on them are then brought close to their PDA shell and quenched. However, the biorecognition behaviors change the probe’s configuration and take the fluorophore far away from the PDA shell. Correspondingly, the fluorescence recovers and its ratio to the constant fluorescence reference is linear to the targets’ concentration. Using a D-catalyst and thrombin as model analytes, the Ru–SiO2@PDA-based nanoplatform shows high sensitivity and good accuracy in the serum sample analysis. Regarding these attractive properties, the Ru–SiO2@PDA nanoplatform provides a new avenue for the accurate and sensitive fluorescence assay of a wide range of targets in complex systems.
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ACS Omega
·
2025
·
IF 4.3
Luyu Lan,Zhipeng Yang,Meijin Lan
Maintenance hemodialysis (MHD) is currently one of the treatments for uremia, but vascular calcification is the most common complication in MHD patients. Research has shown that peripheral blood microRNA-21 is abnormally expressed in uremic patients with vascular calcification. Therefore, it is necessary to detect miRNAs with sensitivity and specificity for early screening of uremia patients with vascular calcification complications. In this work, a light-activated DNA biosensor was constructed by using a catalytic hairpin assembly (CHA) signal amplification strategy and upconversion nanoparticles (UCNPs) for sensitive and specific determination of miRNA-21. A light-activated DNA hairpin (H1) was modified on the superficial layer of the UCNPs to trigger the analyte miRNA. In the presence of both NIR light and miRNA, H1 can hybridize with another DNA hairpin (H2) labeled with BHQ-2 and Cy5 at its two terminals, enabling fluorescence from “OFF” to “ON”. Furthermore, the released analyte miRNA triggers catalytic assembly, establishing an NIR-activated CHA amplification system. The constructed biosensor was used to detect miRNA-21, with a low detection limit (LOD) of 0.25 nM. Additionally, this biosensor can detect miRNA-21 in serum samples, with recoveries ranging from 96.7%–102.0%, suggesting that the current work provides an opportunity to develop NIR-activated signal amplification platforms for sensitive miRNA detection in biological samples.
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高影响因子
TALANTA
·
2025
·
IF 6.1
Zhihui Zeng,Zihan Yan,Hao Jiang,Anyi Li,Junyuan Yang,Xuefei Lv
Prostate cancer is one of the most common malignancies worldwide and a leading cause of cancer-related death in men. MicroRNAs (miRNAs) have emerged as promising and highly specific biomarkers for its detection. However, conventional miRNA-based biosensors face challenges of limited specificity, high false positives in single-target assays, and inadequate sensitivity. Here we developed an integrated pre-amplified DNA logic gate platform with cascaded signal amplification (IPL-CAFS-PCmi) for catalytic fluorogenic sensing of prostate cancer miRNAs. The system uses an AND-gate architecture, combining rolling circle amplification (RCA) for pre amplification, hybridization chain reaction (HCR) for signal enhancement and HCR-assembled multi-component nucleic acid enzymes (MNAzymes) for final cleavage-based amplification. The platform achieved detection limits of 2.21 fM for miR-141 and 2.66 fM for miR-375, with excellent specificity in discriminating single-base mismatches. In diluted human serum, recovery rates ranged from 98.51% to 99.16%, demonstrating strong robustness. By integrating RCA with HCR-mediated MNAzyme assembly in a programmable DNA logic framework, this design provides multi-level cascade amplification at room temperature, significantly improving both sensitivity and specificity. The platform thus represents a versatile tool with strong potential for precision oncology, clinical diagnostics, and point-of-care testing.
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ANALYTICAL SCIENCES
·
2015
·Vol. 31
·期 7
·669-675
·
IF 1.394
Yan Kai,Zhu Yuhan,Cheng Ling,Zhang Jingdong
A novel photoelectrochemical strategy for the sensitive determination of p-nitrophenol (PNP) was developed using a glassy carbon electrode (GCE) modified with CdSe quantum dots (QDs) and DNA composite film (CdSe-DNA/GCE). Various surface analytical techniques, including X-ray diffraction (XRD), transmission electron microscopy (TEM) and scanning electron microscopy (SEM), were employed to characterize the synthesized CdSe QDs and CdSe-DNA modified electrode. The interfacial behaviors of the modified electrodes were analyzed by electrochemical impedance spectroscopy (EIS), and the interaction between PNP and DNA was studied by UV-visible spectrometry. Due to the PNP-DNA interaction, CdSe-DNA/GCE exhibited a sensitive photocurrent response toward PNP under visible-light irradiation. The influencing factors, such as the concentration of DNA used for fabricating CdSe-DNA/GCE and the bias potential applied in the photoelectrochemical measurement, were investigated. Under the optimized conditions, the photocurrent on CdSe- DNA/GCE was linearly increased with the PNP concentration from 0.7 to 50 μmol L −1 , with a detection limit (3 S/N ) of 0.27 μmol L −1 . The proposed photoelectrochemical strategy was successfully applied to monitoring the degradation of PNP.
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MICROBIAL PATHOGENESIS
·
2024
·
IF 3.3
Tongyu Zhang,Yawen Tian,Xiao Zhang,Wenfeng Wang,Yingkai He,Chongbo Ge,Futing Jia,Zhannan Wang,Yanlong Jiang
The H9N2 avian influenza virus (AIV) is spreading worldwide. Presence of H9N2 virus tends to increase the chances of infection with other pathogens which can lead to more serious economic losses. In a previous study, a regulated delayed lysis Salmonella vector was used to deliver a DNA vaccine named pYL233 encoding M1 protein, mosaic HA protein and chicken GM-CSF adjuvant. To further increase its efficiency, chitosan as a natural adjuvant was applied in this study. The purified plasmid pYL233 was coated with chitosan to form a DNA containing nanoparticles (named CS233) by ionic gel method and immunized by intranasal boost immunization in birds primed by oral administration with Salmonella strain. The CS233 DNA nanoparticle has a particle size of about 150 nm, with an encapsulation efficiency of 93.2 ± 0.12 % which protected the DNA plasmid from DNase I digestion and could be stable for a period of time at 37°. After intranasal boost immunization, the CS233 immunized chickens elicited higher antibody response, elevated CD4 + T cells and CD8 + T cells activation and increased T-lymphocyte proliferation, as well as increased productions of IL-4 and IFN-γ. After challenge, chickens immunized with CS233 resulted in the lowest levels of pulmonary virus titer and viral shedding as compared to the other challenge groups. The results showed that the combination of intranasal immunization with chitosan-coated DNA vaccine and oral immunization with regulatory delayed lytic Salmonella strain could enhance the immune response and able to provide protection against H9N2 challenge.
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Frontiers in Toxicology
·
2021
·
IF 0
Hu Xing,Luo Xiaoqiao,Zhou Zhisheng,Wang Rui,Hu Yaqin,Zhang Guimei,Zhang Guowen
1–Naphthaleneacetic acid (NAA), having high-quality biological activity and great yield-increasing potential in agricultural production, is a broad-spectrum plant growth regulator. Although NAA is of low toxicity, it can affect the balance of the human metabolism and damage the body if it is used in high quantity for a long time. In this study, the interaction of NAA with calf thymus DNA (ctDNA) was investigated under simulated human physiological acidity (pH 7.4) using fluorescence, ultraviolet-visible absorption, and circular dichroism spectroscopy combined with viscosity measurements and molecular simulation techniques. The quenching of the endogenous fluorescence of NAA by ctDNA, observed in the fluorescence spectrum experiment, was a mixed quenching process that mainly resulted from the formation of the NAA–ctDNA complex. NAA mainly interacted with ctDNA through hydrophobic interaction, and the binding constant and quenching constant at room temperature (298 K) were 0.60 × 105 L mol−1 and 1.58 × 104 L mol−1, respectively. Moreover, the intercalation mode between NAA and ctDNA was verified in the analysis of melting point, KI measurements, and the viscosity of ctDNA. The results were confirmed by molecular simulation, and it showed that NAA was enriched near the C–G base of ctDNA. As shown in circular dichroism spectra, the positive peak intensity of ctDNA intensified along with a certain degree of redshift, while the negative peak intensity decreased after binding with NAA, suggesting that the binding of NAA induced the transformation of the secondary structure of ctDNA from B-form to A-form. These researches will help to understand the hazards of NAA to the human body more comprehensively and concretely, to better guide the use of NAA in industry and agriculture.
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BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Haihua Wang,Mengjie Li,Yingning Zheng,Tao Hu,Yaqin Chai,Ruo Yuan
Though preferable progresses have been achieved to improve the photoelectric performance of fullerene (C 60 NPs) by sensitized structure in photoelectrochemical (PEC) field, further application inevitably suffers from the inherent scarcities of heavy metal-involved quantum dots as sensitizers containing restricted sensitization effect, complex preparation and biological toxicity. In this work, a PEC biosensor based on [Ru(dcbpy) 2 dppz] 2+ /Rose Bengal dyes co-sensitized C 60 NPs was constructed for ultrasensitive DNA (a fragment sequence of p53 gene) detection. With the merits of low toxicity and accessible operation, [Ru(dcbpy) 2 dppz] 2+ /Rose Bengal dyes exhibited a further sensitization efficiency towards C 60 NPs. Through modifying wide band gap C 60 NPs with two narrower band gap dyes ([Ru(dcbpy) 2 dppz] 2+ and Rose Bengal) to form a cascade-type energy band structure , the photoelectric conversion of C 60 NPs was significantly improved and the visible light absorption was markedly promoted, leading to an exceptional photocurrent signal. Additionally, Nt.BstNB I enzyme-assisted target recycling amplification was employed to convert a limited quantity of target to numerous SiO 2 NPs-labeled DNA sequences (the signal quencher), resulting in a sharp decrement of photocurrent since a dramatic increment of steric hindrance on the modified electrode surface, which was performed to quantitatively estimate target. The proposed PEC biosensor for DNA detection possessed a wide linear range from 0.1 fM to 1 nM with a calculated detection limit of 37 aM. This work opened up an intriguing avenue for determination of various targets such as DNAs, microRNAs and proteins, and exhibited desirable application potential in the clinic researches, cancer therapies and other related subjects.
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