MICROCHIMICA ACTA
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2019
·Vol. 186
·期 5
·1-8
·
IF 5.479
Zhao Chen,Hong Cheng-yi,Lin Zheng-zhong,Chen Xiao-mei,Huang Zhi-Yong
A specific and sensitive colorimetric aptasensor is described for the determination of Malachite Green (MG). It is exploiting the inhibition of the peroxidase-like activity of gold nanoparticles (AuNPs). The AuNPs act as enzyme mimics that catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by H 2 O 2 to yield a dark blue solution. The catalytic activity is inhibited by hexadecyl trimethyl ammonium ion, specifically by cetyltrimethylammonium bromide (CTAB), which causes the aggregation of AuNPs. If a (negatively charged) RNA-aptamer against MG is added, it binds to the positively charged CTAB and prevents aggregation. This enhances the enzyme mimicking activity of the AuNPs and leads to the formation of a dark blue solution. However, in the presence of MG, the aptamer binds to MG, and leads to the aggregation of AuNPs again. The aggregated AuNPs possess a light blue color. A colorimetric method (best performed at 650 nm) was work out that can detect MG in a concentration range from 10 to 500 nmol L −1 . The detection limit based on 3σ/k criterion is 1.8 nmol L −1 . The assay is highly specific and accurate. Recoveries from spiked real samples (aquaculture water) ranged from 80% to 120%.
Based on the inhibition of cetyltrimethyal ammonium ion and the enhancement of RNA-aptamer, the differences of the peroxidase-like activities of AuNPs can be greatly enlarged with and without MG, by which a colorimetric aptasensor can be constructed for the detection of Malachite Green (MG).
研究领域:
分析化学
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纳米技术
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环境科学
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生物化学
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DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2021
·
IF 8.128
Changping Yang,Zhongwei Jiang,Qing Wu,Congyi Hu,Chengzhi Huang,Yuanfang Li,Shujun Zhen
Although a number of nanozymes have been developed, it is still difficult to develop single-component nanozyme with overall high multienzyme-like activities. In this study, the nanosized metal–organic frameworks (nano-MOFs) FePCN (PCN stands for porous coordination network) was synthesized by integrating zirconium and iron ions with different catalytic property on single-component MOFs and exhibited superior intrinsic multienzyme-like activities, namely oxidase-, peroxidase- and phosphatase-mimicking activity. The catalytic active sites of oxidase- and peroxidase-, and phosphatase-like activity of FePCN were Fe-centers and Zr-O clusters, respectively. Based on the intrinsic oxidase-like activity and the similarity of molecular structures between cytochrome P450 oxidase (CYP) cofactors and the organic linker in FePCN, FePCN exhibited high CYP-like activity to catalyze the oxidation of hypotensive drug 1,4-dihydropyridine (1,4-DHP) into diethyl 2,6-dimethylpyridine-3,5-dicarboxylate (DDPD) and the yield of DDPD reached over 80%. Moreover, as peroxidase- and phosphatase-mimics, FePCN was successfully applied to detecting H 2 O 2 under neutral condition and catalyzing the dephosphorylation of adenosine triphosphate (ATP), respectively. This study provides a feasible way for rational design one-component nanomaterials as multienzyme-mimics.
研究领域:
纳米技术
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材料科学
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生物化学
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药物化学
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2021
·
IF 6.558
Lvxia Zhang,Hui Ouyang,Dan Zhang,Zhifeng Fu
Novel cobalt-based metal-organic frameworks (Co MOFs) were synthesized by a facile “controlled synthesis” strategy. The MOFs displayed superior catalytic performance on the chemiluminescent (CL) reaction between N -(4-aminobutyl)- N -ethylisoluminol (ABEI) and H 2 O 2 . UV–vis absorption, CL spectrum, ESR, and radical scavenger experiments were conducted for clarifying the catalytic mechanism of Co MOFs. All results revealed that Co MOFs can accelerate decomposition of H 2 O 2 and production of OH • , O 2 • − as well as 1 O 2 radicals. The rapid reaction between these reactive oxygen species and ABEI resulted in the generation of ABEI-ox∗. The excited-state oxidation product emitted a very intensive CL signal with a maximal emission wavelength of 430 nm as it returned to the ground state. To explore their application potential in CL assay, Co MOFs were used as powerful CL reaction catalyst for establishing a very sensitive method for immunoassay of aflatoxin B 1 . The detection range was 0.05–60 ng mL −1 , and the limit of detection was 4.3 pg mL −1 . The result for detecting herbal medicine samples demonstrates the acceptable reliability of the Co MOFs-based CL immunoassay. The proof-of-principle work verifies the application potential of Co MOFs on boosting intensive CL signal, and meets the demand for high sensitivity in various bioassay fields.
研究领域:
分析化学
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材料科学
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生物化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2020
·
IF 2.87
Shujuan Guo,Yanjie Zhang,Yuansong Ye,Jinzhe Song,Mengru Li
Synthesis of a Ti-incorporated zeolite using a simple and economical method has recently become a focus of attention. The direct hydrothermal synthesis of Ti-MWW is most commonly applied; however, it is challenging to perform and exhibits low titanium utilization. An innovative strategy of synthesizing Ti-MWW is proposed in the present study by simply treating the ERB-1 precursor of an MWW-type boron silicate with a H2TiF6/HNO3 solution. This significantly shortens the Ti grafting process from 5 days to only a few hours and reduces the use of the structure-directing agent hexamethyleneimine (HMI); furthermore, no extraframework Ti is observed in the precursor, indicating good atomic economy. Typically, a piperidine (PI)-treated sample Ti-MWW2–1-PI exhibits a higher conversion (76.6%) than the original Ti-MWW (44.8%) in the epoxidation of 1-hexene. X-ray diffraction (XRD), inductively coupled plasma (ICP), and transmission electron microscopy (TEM) techniques are used to explain in detail the probable mechanism underlying the incorporation of Ti species into the MWW framework. X-ray photoelectron spectroscopy (XPS) is employed to study the coordinate state of the Ti and F species in the samples after treatment with a piperidine solution. This method can be applied to synthesize other kinds of lamellar-structured zeolites with heteroatoms.
研究领域:
催化化学
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材料科学
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DOI
PubMed
高影响因子
Catalysts
·
2026
·Vol. 16
·期 5
·417
·
IF 4.5
Ziyan Mi,Huayun Long,Yuhua Jia,Yue Ma,Cuilan Miao,Yan Xie,Xiaomei Zhu,Jiahui Huang
Developing effective catalysts for the epoxidation of propylene with H2and O2holds significant scientific and industrial significance. This study synthesized a series of Au/TS-1 catalysts modified with alkali metals (Na+, Cs+) and carefully examined their impact on gas-phase propylene epoxidation, with particular attention to the role of anions. The optimal Au–CsC(1:10)/TS-1 catalyst (Cs2CO3modified, Au/Cs molar ratio = 1:10) achieves a propylene conversion of 16.8%, a PO selectivity of 88.5%, an H2efficiency of 40.8%, a record PO formation rate of 383.9 gpo·kgcat−1·h−1, and unprecedented long term stability (>380 h without deactivation). To the best of our knowledge, no previous study has simultaneously achieved such balanced and outstanding performance across all these key indicators. Comprehensive characterization reveals that Cs+modification suppresses side reactions and coke formation, increases microporosity, tunes surface acid–base properties and hydrophobicity, restricts Au particle size, and stabilizes both Au0and tetra coordinated Ti sites, thereby inhibiting H2O2decomposition and PO isomerization while greatly enhancing reaction efficiency. This holistic advancement represents a significant leap forward for Au based catalysts in gas phase propylene epoxidation, offering both a theoretical foundation and practical guidance for the development of high performance epoxidation catalysts.
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2025
·
IF 7.7
Lei Shen,Ze Zhang,Xinying Guo,Zelin Zhong,Jia Zhang,Chengcheng Zhang,Hongmei Liu,Shuxiang Wang,Jinchao Zhang,Caixia Yin,Yutao Yang
Epilepsy is a chronic brain disease with various causes, among which oxidative stress-induced neuronal damage is a significant pathological factor and has been widely studied as one of the key pathogenic mechanisms. Hydrogen peroxide (H₂O₂), a representative reactive oxygen species (ROS), plays an important role in epilepsy. However, real-time imaging capable of revealing the distribution of H₂O₂ in the living brain remains limited. To address this, we developed a feasible fluorescent probe library based on the unique reaction between the α-ketoester moiety and H₂O₂, combined with dicyanoisophorone derivatives possessing excellent optical performance. In consequence, DCI-BT-PY was optimized as a novel fluorescent probe for H₂O₂ through parallel testing of its physicochemical and optical properties. Notably, due to its good blood-brain barrier (BBB) penetration, DCI-BT-PY was used to monitor H₂O₂ upregulation in the brains of epileptic mice, revealing a close relationship between H₂O₂ and epilepsy. Furthermore, using the Sombati epilepsy model of hippocampal neuronal cells and the mouse model of epilepsy, the neuroprotective effect of curcumin on oxidative stress injury of nerve cells was explored, showing that it may alleviate epilepsy by activation of the Nrf2/HO-1 signaling pathway for suppressing the neurotoxic damage. This finding offers new insights into the pathogenesis and treatment of epilepsy. Additionally, this work provides a reliable tool for evaluating H 2 O 2 fluctuations in the brains of epilepsy-induced mice, thereby establishing a solid foundation for antioxidant therapies targeting epilepsy.
研究领域:
神经科学
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药理学
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生物化学
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分子生物学
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医学影像学
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神经病学
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DOI
高影响因子
Journal of Water Process Engineering
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2024
·
IF 6.3
Feng Liu,Huanhuan Zhang,Nian Liu,Junxia Wang,Maolin Chen,Feiyue Qian
Wafer surface treatment is a crucial step in the integrated circuit packaging process, which would generate high-strength organic wastewater using propylene glycol monomethyl ether (PGME) as a photoresistor thinner. This study investigated the performance of Fenton oxidation and catalytic ozonation (O 3 /H 2 O 2 /FeOOH) for removing refractory organics in wafer surface treatment wastewater by optimizing operation and combining utilizations. Gas chromatography–mass spectrometry was employed to analyze intermediates composition in the effluents treated by various advanced oxidation processes , and the toxicity of organics was predicted using the Quantitative Structure-Activity Relationship tool. Fenton oxidation outperformed O 3 /H 2 O 2 /FeOOH in the removal efficiencies of chemical oxygen demand (COD) and PGME, but produced 9.60 kg·m −3 of chemical sludge . Additionally, 3-hydroxy- N -phenylaniline, an acutely toxic compound, was dominant in the intermediates of Fenton oxidation, indicating the ecological risks of chemical sludge. O 3 /H 2 O 2 /FeOOH followed by Fenton oxidation (O 3 /H 2 O 2 /FeOOH + Fenton) exhibited higher total organic carbon removal (77.7 %) with less chemical sludge production (8.47 kg·m −3 ) than the reverse combination (Fenton + O 3 /H 2 O 2 /FeOOH). Although the intermediates of aniline, ketone, and ester exhibited considerable acute and developmental toxicity , the significant improvement in biodegradability of the effluents (BOD 5 /COD ratio of 0.36 > 0.02 for raw wastewater) favored subsequent biological treatment. These findings provide guidance for the cost-effective treatment of wafer surface treatment through rational designing combined processes.
研究领域:
环境科学
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化学工程
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
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2024
·
IF 4.9
Wenfang Huang,Shulong Wang,Min Wu,Xinping Huang,Qifei Mo,Jiayao Xu,Rong Wang,Qingmin Wei
Hydrogen peroxide (H 2 O 2 ) plays a crucial role as a signaling molecule within cells. The concentration of H 2 O 2 differs in inflammation, cancer, cardiovascular and cerebrovascular diseases and neurodegenerative diseases compared to normal tissue. Therefore, the accurate determination of H 2 O 2 is important for the diagnosis and understanding of pathogenesis of disease. However, to accurately determine H 2 O 2 levels in vivo, highly sensitive biosensors and deep tissue penetrating imaging techniques are required. To address this challenge, we developed a biocatalytic porous structure composed of metal–organic frameworks (MOFs) that encapsulates horseradish peroxidase (HRP) and 3, 3′, 5, 5′-Tetramethylbenzidine (TMB) in situ, enabling biomineralization while maintaining the catalytic activity and stability of natural enzymes. This leads to the formation of a hypersensitive biological probe BSA@HRP@TMB@ZIF-8 (BHTZ). BHTZ is an activatable near-infrared probe and its absorption signal in the second near-infrared window (1030 nm) is activated in the presence of H 2 O 2 . The findings indicate that the BHTZ probe has high sensitivity, low toxicity and high stability, and can be utilized to detect H 2 O 2 levels in mice with drug-induced liver injury, such as that caused by lipopolysaccharide (LPS) in vivo. These results not only provide a highly sensitive and deep imaging method for monitoring physiological changes in H 2 O 2 levels in vivo, but also broaden the potential applications of MOFs in biomedical research and diagnostics.
研究领域:
生物医学工程
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分析化学
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分子生物学
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成像科学
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产品关联
DOI
高影响因子
ACS Omega
·
2024
·
IF 3.7
Yuting Wang,Xiaochen Shi,Wenwen Li,Yajun Wang,Wenqing Yao
The generation of H2O2 through photocatalysis is increasingly recognized as a viable approach for addressing the energy and environmental challenges encountered in industrial production processes. In this research, we synthesized ultrathin (1 nm) poly(heptazine imide) (PHI) nanosheets as a photocatalyst by a one-step KCl molten salt process. The utilization of Fourier transform infrared and X-ray photoelectron spectroscopy substantiated that the interlayer-bonded potassium atoms induce polarization in water molecules, facilitating the attachment of hydroxyl groups on the surfaces of nanosheets. These groups serve as self-sacrificing entities, promoting the reaction that leads to the generation of H2O2. The preparation temperature and KCl doping amount factors for the H2O2 generation rate were investigated, and the mechanism of the KCl-bonded structure on photogeneration charge separation transport was analyzed. Owing to the elevated crystallization and the presence of surface self-sacrificing hydroxyl groups, the rate of H2O2 production reaches 6117.5 and 308.35 μmol·g–1·h–1 under visible-light irradiation (λ ≥ 420 nm) in isopropanol solution and pure water, respectively. These rates are 30 and 18.7 times higher than those observed for bulk g-C3N4, respectively. The photocatalytic kinetic processes for H2O2 formation and decomposition were also calculated to investigate the catalyst activities.
研究领域:
材料科学
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环境科学
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化学
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产品关联
DOI
PubMed
高影响因子
WATER RESEARCH
·
2019
·
IF 7.913
Ying Wan,Pengchao Xie,Zongping Wang,Jiaqi Ding,Jingwen Wang,Songlin Wang,Mark R. Wiesner
In this study, ultraviolet based advanced oxidation processes (UV-AOPs) including UV/persulfate (UV/PS), UV/chlorine, and UV/H 2 O 2 were employed to alleviate ultrafiltration membrane fouling during the treatment of algae-laden water. The results show that UV/PS pretreatment exhibited the best performance on fouling control, followed by the UV/H 2 O 2 pretreatment . The fouling mitigation performance improved with the increase of oxidant dose. However, UV/chlorine pretreatment aggravated membrane fouling, and the irreversible fouling resistance increased by five times compared with that of raw water. The dissolved organic carbon (DOC) in the algae-laden solution was reduced after UV/PS pretreatment, while either UV/chlorine or UV/H 2 O 2 pretreatment had little influence on the DOC of feed water. UV/PS and UV/H 2 O 2 pretreatments were effective in the degradation of fluorescent compounds, thus reducing the deposition of organic matter on the membrane surface. Additionally, the decreased concentration of hydrophobic organics, algal cells, and debris in feed water after UV/PS pretreatment was also contributed to the fouling alleviation. The aggravated irreversible fouling after UV/chlorine pretreatment was probably ascribed to the increased accumulation of hydrophobic fractions in the membrane pores. Modeling result indicates that membrane fouling during the filtration of raw algae-laden water was dominated by intermediate blocking and cake filtration mechanisms. Both UV/PS and UV/H 2 O 2 pretreatments transformed the combined fouling mechanism into standard blocking, while UV/chlorine pretreatment aggravated the pore blocking in the initial filtration period.
研究领域:
环境科学
·
水处理工程
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2019
·
IF 3.973
Tingting Liu,Jing Tian,Lin Cui,Qingyun Liu,Lili Wu,Xiaomei Zhang
Nanozymes, nanomaterial-based artificial enzymes, have attracted researchers’ enormous interest due to their unique properties compared with natural enzymes. To mimic the catalytic function of natural enzymes, designing high-efficient, novel nanozymes is crucial yet challenging task. In this article, we described the synthesis and functions of a metalloporphyrin-based porous organic polymer, namely FePPOPs-SO 3 H. FePPOPs-SO 3 H was synthesized effortlessly via an extensive aromatic electrophilic substitution and the following sulfonation reactions. This strategy was cost-efficient without the participation of precious metal catalysts. The resultant FePPOPs-SO 3 H is intriguing since the framework itself is constructed by covalently linked porphyrin units, which could serve as a built-in catalyst and strengthen the stability of polymer. With sulfonic acid side groups, FePPOPs-SO 3 H is well water-dispersive. Owing to these unique characteristics, FePPOPs-SO 3 H exhibited excellent peroxidase-like activity toward a classical peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB) to produce a blue product only within 20 s. The peroxidase-mimicking performance of FePPOPs-SO 3 H outperforms the ferric porphyrin monomer and normal Fe 3 O 4 nanoparticles. Based on the excellent catalytic activity of FePPOPs-SO 3 H, two visual colorimetric sensors for ultrafast detecting H 2 O 2 and glucose, respectively, were constructed with a wide linear range of 50–1800 μM (for H 2 O 2 ) and 200–1500 μM (for glucose), as well as a relative lower limit of detection (LOD) [26.70 μM (for H 2 O 2 ) and 16.38 μM (for glucose)]. Our strategy highlights opportunities for the design of new metalloporphyrin-based porous organic polymers with built-in catalytic skeletons and inherently excellent peroxidase-mimicking performance.
研究领域:
材料科学
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纳米技术
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分析化学
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生物材料
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生物化学
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DOI
PubMed
高影响因子
Redox Biology
·
2026
·
IF 16.2
Li He,Jia-Mi Li,Meng-Ting Li,Cai-Ju Zhang,Yu-Fan Lv,Jiao-Jiao Ma,Mao-Lin Zou,Bo Wu,Shi-Wen Huang,Gang Liu,Yong-Chang Wei,Dan Xu,Kai Deng
The sequential monitoring of two pathological processes has clinical significance for early detection of drug-induced acute kidney injury (AKI), but remains challenging due to the lack of spatiotemporal probes. Here, we developed a hydrogen peroxide (H 2 O 2 ) and myeloperoxidase (MPO) programmable responsive magnetic resonance imaging (MRI) probe (PAH-Gd) for spatiotemporal monitoring of renal oxidative stress and inflammation. Upon exposure to H 2 O 2 , PAH-Gd transformed into a kidney-targeting moiety, H–Gd, which selectively accumulated in kidneys and moderately enhanced renal T1-weighted imaging (T1 WI) signal. Subsequently, the H–Gd could be oxidized by MPO to form dimers or adducts with nearby proteins, further enhancing renal T1 WI signal. We found that PAH-Gd could perform spatiotemporal monitoring of renal oxidative stress and inflammation at 12 h and 24 h post-Cisplatin (DDP) administration, enabling early detection of DDP-induced AKI at least 60 h earlier than that of standard clinical assays and permitting dynamic monitoring of renal injury progression. The spatiotemporal T1 WI overcame the limitations in detecting diverse pathological processes with a single MRI probe, accurately reporting the drug-induced AKI in the early stage and contributing to AKI prevention and treatment in the clinic.
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DOI
PubMed
高影响因子
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