RSC Advances
·
2017
·Vol. 7
·期 29
·17711-17719
·
IF 3.108
Yingling Miao,Ruirui Yang,David Y. B. Deng,Li-Ming Zhang
Excellent biocompatibility and bioactivity are necessary requirements for a scaffold for nerve repair and regeneration. Natural plant protein zein was chosen as the primary material and poly(L-lysine), which is composed of common amino acids in the human body, was used to modify it. Poly(L-lysine) modified zein (ZPLL) with different PLL contents of 1.46%, 3.57%, and 6.18% was synthesized and nanofibrous membranes were prepared by electrospinning. The hydrophilicity of the membranes improved with an increase of PLL content. The biodegradability of the membranes was proved by in vitro experiments. Compared with pure zein membranes, ZPLL membranes can efficiently improve cell viability, adhesion, proliferation, and differentiation of neural stem cells (NSCs) and the effect of PLL content was further investigated. The results show that when the PLL content was 3.57%, cell adhesion and proliferation proved to be the best and most differentiated toward mature neurons with extensive neurite formation and astrocytes rather than oligodendrocytes. The ZPLL was made into nerve conduits for future study and they may be a promising biomaterial for nerve repair and regeneration.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Yuanqi Duan,Zhengyu Hu,Long Jin,Tieqiang Zong,Xiaohui Zhang,Yanan Liu,Pengcheng Yang,Jinfeng Sun,Wei Zhou,Gao Li
The degradation of Belamcanda chinensis (L.) DC. polysaccharides was carried out by five concentrations of trifluoroacetic acid (TFA) (1–5 mol/L), and their physicochemical properties, degradation kinetics and anticomplementary activity were investigated. The findings revealed a notable reduction in the molecular weight of BCP, from an initial value of 2.622 × 10 5 g/mol to a final value of 6.255 × 10 4 g/mol, and the water solubility index increased from 90.66 ± 0.42 % to 97.78 ± 0.43 %. The degraded polysaccharides of B. chinensis exhibited a comparable monosaccharide composition comprising Man, GalA, Glc, Gal, and Ara. As the concentration of TFA increased, the degradation rate constant increased from 1.468 × 10 −3 to 5.943 × 10 −3 , and the process followed the first-order degradation kinetic model (R 2 > 0.97) and the random fracture model (R 2 > 0.96). Furthermore, the five degraded polysaccharides still exhibit good thermal stability. In vitro experiments showed that DBCP-3 exhibited more potent anticomplementary activity than the original polysaccharides and positive drugs, which was strongly correlated with its Mw ( r = 0.6–0.8), inhibiting complement activation by blocking C2 and C4. These results indicated that TFA degradation has a positive effect on polysaccharides, of which DBCP-3 is expected to treat diseases involving hyperactivation of the complement system.
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MOLECULES
·
2024
·Vol. 29
·期 5
·1046
·
IF 4.2
Xiurong Ju,Tao Chen,Yutao Ding,Dan Yu,Jingyu Zhang,Ruyuan Zhang,Yang Zhang,Xinyu Wang,Tao Xu,Jiayou Li
TheHibiscus manihotL. (HML) Medic, an edible hibiscus of the Malvaceae family, is abundant with flavonoids. The study investigated howRhizopus-arrhizus-31-assisted pretreatment affects the extraction and bioactivity of flavonoids from HML. The fiber structure of the fermented flavonoid sample (RFF) appears looser, more porous, and more disordered than the unfermented flavonoid sample (RUF). RFF demonstrates milder conditions and yields higher extraction rates. According to the Box–Behnken response surface optimization experiment, the optimal conditions for RFF include a material–liquid ratio of 1:41 g/mL, a 2 h extraction time, a 57% ethanol concentration, and an extraction temperature of 800 °C, resulting in a 3.69% extraction yield, which is 39.25% higher than that of RUF. Additionally, RFF exhibits greater activity than RUF in the radical-scavenging system. The IC50 values for DPPH, OH, and ABTS radicals are 83.43 μg/mL and 82.62 μg/mL, 208.38 μg/mL and 175.99 μg/mL, and 108.59 μg/mL and 75.39 μg/mL for RUF and RFF, respectively. UPLC-QTOF-MS analysis of the active components in the HML flavonoid sample revealed significant differences in the chromatograms of RUF and RFF, indicating that biofermentation led to substantial changes in composition and content from HML.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 3
·1-11
·
IF 5.3
Zhang Wei,Zhang De,Wang Pu,Li Xiaoming,Wang Zhetao,Chen Qiang,Huang Jie,Yu Zhi,Guo Fei,Liang Pei
An ultrasensitive surface-enhanced Raman spectroscopy (SERS) aptamer sensor (aptasensor) using a noble metal nanoparticle-magnetic nanospheres composite was developed for L-theanine detection. It makes use of Fe 3 O 4 @Au MNPs and Au@Ag NPs embedded with the Raman reporter 4-mercaptobenzoic acid (4MBA). Au@4MBA@Ag NPs modified by aptamer and Fe 3 O 4 @Au MNPs modified by cDNA created the aptasensor with the strongest Raman signal of 4MBA through the specific binding of the aptamer. With the preferred binding of L-theanine aptamer to L-theanine, Au@4MBA@Ag NPs were released from Fe 3 O 4 @Au MNPs, causing a linear decrease in SERS intensity to achieve the SERS detection of the L-theanine. The SERS peak of 4MBA at 1078 cm −1 was used for quantitative determination. SERS intensity showed a good log-linear relationship within the range 10 −10 to 10 −6 M of L-theanine. The aptasensor has a high selectivity for L-theanine compared with other twelve tested analytes. Hence, this aptasensor is a promising analytical tool for L-theanine detection. The developed method was applied to the analysis of real samples, demonstrating excellent performance. The comparison with the standard liquid chromatography mass spectrometry method showed an error within 20%. Graphical abstract
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Frontiers in Bioengineering and Biotechnology
·
2024
·
IF 4.3
You Xiaoxiao,Wang Zhongke,Wang Li,Liu Youbo,Chen Hongmei,Lan Xiaorong,Guo Ling
The construction of an antibacterial biological coating on titanium surface plays an important role in the long-term stability of oral implant restoration. Graphene oxide (GO) has been widely studied because of its excellent antibacterial properties and osteogenic activity. However, striking a balance between its biological toxicity and antibacterial properties remains a significant challenge with GO. ε-poly-L-lysine (PLL) has broad-spectrum antibacterial activity and ultra-high safety performance. Using Layer-by-layer self-assembly technology (LBL), different layers of PLL/GO coatings and GO self-assembly coatings were assembled on the surface of titanium sheet. The materials were characterized using scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and contact angle test. The antibacterial properties of Porphyromonas gingivalis (P.g.) were analyzed through SEM, coated plate experiment, and inhibition zone experiment. CCK-8 was used to determine the cytotoxicity of the material to MC3T3 cells, and zebrafish larvae and embryos were used to determine the developmental toxicity and inflammatory effects of the material. The results show that the combined assembly of 20 layers of GO and PLL exhibits good antibacterial properties and no biological toxicity, suggesting a potential application for a titanium-based implant modification scheme.
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Foods
·
2024
·Vol. 13
·期 12
·1865
·
IF 4.7
Jiayu Lu,Zheng Jiang,Jingjie Dang,Dishuai Li,Daixin Yu,Cheng Qu,Qinan Wu
Chamomile has become one of the world’s most popular herbal teas due to its unique properties. Chamomile is widely used in dietary supplements, cosmetics, and herbal products. This study aimed to investigate the volatile aromatic components in chamomile. Two analytical techniques, gas chromatography–mass spectrometry (GC-MS) and an ultra-fast gas chromatography electronic nose, were employed to examine samples from Xinjiang (XJ), Shandong (SD), and Hebei (HB) in China, and imported samples from Germany (GER). The results revealed that all chamomile samples contained specific sesquiterpene compounds, including α-bisabolol, bisabolol oxide, bisabolone oxide, and chamazulene. Additionally, forty potential aroma components were identified by the electronic nose. The primary odor components of chamomile were characterized by fruity and spicy notes. The primary differences in the components of chamomile oil were identified as (E)-β-farnesene, chamazulene,α-bisabolol oxide B, spathulenol andα-bisabolone oxide A. Significant differences in aroma compounds included geosmin, butanoic acid, 2-butene, norfuraneol,γ-terpinene. This study demonstrates that GC–MS and the ultra-fast gas chromatography electronic nose can preliminarily distinguish chamomile from different areas, providing a method and guidance for the selection of origin and sensory evaluation of chamomile. The current study is limited by the sample size and it provides preliminary conclusions. Future studies with a larger sample size are warranted to further improve these findings.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Yun Tian,Na Wang,Huanyu Liu,Tongxin Qiu,Chuan Chen,Xia Liu,Yuanqiang Zhu
Curcuminoids originated from the plant Curcuma longa L. show high medical values. Herein, ionic liquids (ILs) were synthesized using fatty acids and alcohol amines to extract curcuminoids. Through single-factor experiments and response surface methodology (RSM) optimization, the extraction amount of curcuminoids by [TEA][FA-C 7 ] aqueous solution reached 34.89 mg/g at a liquid-solid ratio of 16.36 mg/g and an extraction temperature of 36 °C, surpassing that of methanol. The extraction capacity of [TEA][FA-C 7 ] to curcuminoids was 299.33 mg/g. Furthermore, the separation of extracts and recovery of fatty acids were studied. Quantum chemical calculations and molecular dynamics simulations were performed to investigate the extraction mechanism profoundly. This study not only proposed a novel green solvent for the extraction of curcuminoids from the plant Curcuma longa L. but also provided the possibility of applying fatty acid-based ILs to extract natural medicine efficiently.
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FIBERS AND POLYMERS
·
2024
·
IF 2.2
Zhang Qi,Yang Ce,Gu Mingqi,Qin Shaoxuan,Yin Jianhua,Wang Xiangrong,Zhang Wei
The integration of natural dyes and biomass-based dyeing auxiliaries holds huge potential to address the environmental sustainability challenges, which the textile industry encounters. This research employed Portulaca oleracea L. extract as a natural dye, utilizing laccase as a catalyst for polymerizing the dye, thereby creating a precursor that enhances its eco-friendly dyeing attributes on cotton fabrics. The initially exhibited suboptimal direct dyeing performance on cotton fabrics, resulting in a faint yellow hue and a K/S value of 0.66. However, following laccase catalysis, the depth of color on the dyed fabric significantly increased, with a K/S value reaching 4.41, and the dye uptake rate increased from 14.54% to 33.69%. The resulting dyed fabric exhibited excellent fastness to soap washing, light, and rubbing, all scoring above levels 4 to 5. Analysis of dyeing kinetics and adsorption models indicated that the dyeing process was consistent with both the pseudo-first-order and pseudo-second-order kinetic models, suggesting physical and chemical bonding between the precursor and the fiber. Further investigations employing ultraviolet spectrum analysis, matrix-assisted laser desorption/ionization time of flight mass spectrometry, and gas chromatography-mass spectrometer revealed the fundamental composition of the natural dye, the molecular weight, and structural characteristics of the precursor, substantiating that the catalytic mechanism involves laccase-catalyzed oxidation. Additionally, the dyed cotton yielded an ultraviolet protection factor value of 230 and a long-lasting herbal fragrance, accurately identified by an electronic nose. These results confirm the viability of intensifying fabric color using the laccase-catalyzed system. This technique significantly expands the environmental applications of its extract.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2025
·Vol. 26
·期 4
·1558
·
IF 4.9
Jiabin Huang,Haotian Wu,Ranran Gao,Lan Wu,Mengyue Wang,Yang Chu,Yuhua Shi,Li Xiang,Qinggang Yin
L-ascorbic acid (vitamin C, AA) is widely present in plants, but humans lack the ability to synthesize it independently. As a potent reducing agent, AA is susceptible to oxidation, making the enhancement of its stability crucial. 2-O-β-D-glucopyranosyl-L-ascorbic acid (AA-2βG) is a stable natural derivative of AA with glycosylation, initially discovered in the fruits ofLycium barbarum. Understanding the biosynthesis of AA-2βG is crucial for enhancing its production inL. barbarum. While the established biosynthesis pathway of AA constitutes the upstream of AA-2βG biosynthesis, the conclusive step of β-glycosylation remains unclear. We identified aL. barbarumcultivar by UPLC, ZN01, with a high content of AA-2βG, and compared its leaves, immature fruits, and mature fruits to a normal AA-2βG contentL. barbarumcultivar for metabolomic and transcriptomic analysis. The RNA-seq and RT-qPCR analysis revealed that the expression levels of genes involved in the AA biosynthesis pathway did not consistently correlate with AA-2βG content, suggesting that the final glycosylation step may be a key determinant of AA-2βG accumulation. Subsequently, utilizing phylogenetic and co-expression analysis, we identified ten UDP-glycosyltransferases (UGTs) and three β-glucosidases (BGLUs) which may be involved in the crucial step of the conversion from AA to AA-2βG, and the UGTs’ activities were predicted through molecular docking. Lastly, we speculated that the presence of the glycosylation process of AA might have a crucial role in maintaining AA homeostasis inL. barbarum, and deliberated on potential correlations between AA, carotenoids, and anthocyanins. Our integrated multi-omics analysis provides valuable insights into AA-2βG biosynthesis inL. barbarum, identifying thirteen candidate genes and highlighting the complex interplay between AA, carotenoids, and anthocyanins. These findings have implications for improving AA-2βG content inL. barbarum.
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MOLECULES
·
2024
·Vol. 29
·期 24
·6011
·
IF 4.2
Ting Zhou,Luyao Zang,Xia Zhang,Xia Liu,Zijie Qu,Guodong Zhang,Xiufeng Wang,Fang Wang,Zhiqing Zhang
Gold nanoclusters (AuNCs) have been widely investigated because of their unique photoluminescence properties. However, the applications of AuNCs are limited by their poor stability and relatively low fluorescence. In the present work, we developed nanocomposites (L-Cys-AuNCs@ZIF-8) with high fluorescence and stability, which were constructed by encapsulating the water-dispersible L-Cys-AuNCs into a ZIF-8 via Zn2+-triggered growth strategy without high temperature and pressure. The maximum emission wavelength of the L-Cys-AuNCs@ZIF-8 composite was at 868 nm, and the fluorescence intensity of L-Cys-AuNCs@ZIF-8 was nearly nine-fold compared with L-Cys-AuNCs without the ZIF-8 package. The mechanism investigation by fluorescence spectroscopy and X-ray photoelectron spectroscopy showed that L-Cys-AuNCs@ZIF-8 impeded ligand rotation, induced energy dissipation, and diminished the self-quenching effect, attributing to the spatial distribution of L-Cys-AuNCs. Based on the high fluorescence efficiency of L-Cys-AuNCs@ZIF-8, a “signal off” detective platform was proposed with copper ions as a model analyte, achieving a sensitive detection limit of Cu2+at 16.7 nM. The quenching mechanism was confirmed, showing that the structure of the L-Cys-AuNCs@ZIF-8 nanocomposites was collapsed by the addition of Cu2+. Attributing to the strong adsorption ability between copper ions and pyridyl nitrogen, the as-prepared L-Cys-AuNCs@ZIF-8 was shown to accumulate Cu2+, and the Zn2+in ZIF-8 was replaced by Cu2+.
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Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Yuxin Wang,Yueqi Chen,Fengjun Shan,Tingting Zhang,Zhenbin Zhang,Meijun Liu
Hexavalent chromium (Cr(VI)) pollution in aquatic environments has triggered constant worries. As one of the popular adsorbents, graphene oxide (GO) still confronts shortcomings in the Cr(VI) removal, including easy aggregation, limited adsorption amounts, monotonous type of functional groups, constant negative charge and unable valence conversion. Herein, L-glutamic acid (L-Glu) was utilized for GO functionalization through the solvothermal method to fabricate L-Glu/GO with the trait of anti-stacking for overcoming the above-mentioned drawbacks. Results proved that L-Glu/GO possessed the relatively large specific surface area and average pore diameters, various functional groups and variable surface charges. The as-prepared L-Glu/GO exhibited satisfying adsorption amounts with the changeable acid environment, adsorbent dosages and initial Cr(VI) concentrations. Besides, Cr(VI) adsorption process conformed to the pseudo-second-order kinetic model and the Langmuir isotherm model with q m for Cr(Ⅵ) of 71.12 mg·g –1 , almost 27 times higher than that of GO (2.64 mg·g –1 ) in pH = 2. The adsorption thermodynamics result portrayed the adsorption process was a spontaneous endothermic process. Additionally, L-Glu/GO possessed excellent reusability with q e of Cr(Ⅵ) of 57.19 mg·g –1 after five cycles, with the economic cost of ¥0.498·g –1 . More importantly, Cr(VI) was changed into Cr(III) during adsorption to accomplish the toxicity elimination due to existence of amine groups from L-Glu. On the whole, pore filling, anion-π interaction, electrostatic interactions, adsorption-reduction and coordination interaction jointly contributed to the Cr(VI) adsorption. This work provided the strategy for effectively enhancing the Cr(VI) adsorption capacity of GO and deepened the adsorption mechanism towards hazardous metal ions elimination.
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CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Bin Zhao,Yu Ren,Kexin Zhang,Yuchuan Dong,Keke Wang,Nannan Zhang,Jing Li,Maosen Yuan,Jinyi Wang,Qin Tu
Diabetes-related slow healing of wounds is primarily driven by bacterial infections and angiogenesis disorder and presents a substantial hurdle in clinical treatment. To solve the above problems, an advanced multifunctional hydrogel system based on natural polymer was created here to facilitate wound healing in patients with chronic diabetes. The prepared dressing was composed of an outer hydrogel containing polyvinyl alcohol and hydroxypropyl methyl cellulose in dimethyl sulfoxide and water as binary solvents, and an inner hydrogel containing chitosan quaternary ammonium salt , flaxseed gum, and polyvinyl alcohol . Thus, a polysaccharide based bilayer hydrogel (BH) with superior mechanical strength and biocompatibility was created. This bilayer hydrogel could easily bind to dynamic tissue surfaces, thereby generating a protective barrier. Meanwhile, L-arginine-modified polyoxometalate (POM@L-Arg) nanoclusters were loaded in the inner hydrogel. They released NO when stimulated by the peroxide microenvironment of diabetic wounds. NO as a signal molecule regulated vascular tension and promoted cell proliferation and migration. Additionally, because of the synergistic effect of NO and the chitosan quaternary ammonium salt, the hydrogel system exhibited excellent antibacterial performance. The NO released reduced the levels of proinflammatory factors IL-6 and TNF-α in the diabetic wounds, which thus accelerated wound healing. In short, BH + POM@L-Arg is expected to serve as an ideal wound dressing as it exerts a good promotion effect on diabetes-related wound healing.
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