ANALYTICAL CHEMISTRY
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2021
·
IF 6.986
Jiamin Wu,Xia Zhou,Ping Li,Xiaoling Lin,Jinhua Wang,Ziwei Hu,Pengcheng Zhang,Dong Chen,Huaihong Cai,Reinhard Niessner,Christoph Haisch,Pinghua Sun,Yun Zheng,Zhengjin Jiang,Haibo Zhou
Sensitive and simultaneous detection of multiple cancer-related biomarkers in serum is essential for diagnosis, therapy, prognosis, and staging of cancer. Herein, we proposed a magnetically assisted sandwich-type surface-enhanced Raman scattering (SERS)-based biosensor for ultrasensitive and multiplex detection of three hepatocellular carcinoma-related microRNA (miRNA) biomarkers. The biosensor consists of an SERS tag (probe DNA-conjugated DNA-engineered fractal gold nanoparticles, F-AuNPs) and a magnetic capture substrate (capture DNA-conjugated Ag-coated magnetic nanoparticles, AgMNPs). The proposed strategy achieved simultaneous and sensitive detection of three miRNAs (miRNA-122, miRNA-223, and miRNA-21), and the limits of detection of the three miRNAs in human serum are 349 aM for miRNA-122, 374 aM for miRNA-223, and 311 aM for miRNA-21. High selectivity and accuracy of the SERS biosensor were proved by practical analysis in human serum. Moreover, the biosensor exhibited good practicability in multiplex detection of three miRNAs in 92 clinical sera from AFP-negative patients, patients before and after hepatectomy, recurred and relapse-free patients after hepatectomy, and hepatocellular carcinoma patients at distinct Barcelona clinic liver cancer stages. The experiment results demonstrate that our SERS-based assay is a promising candidate in clinical application and exhibited potential for the prediction, diagnosis, monitoring, and staging of cancers.
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ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Jiangtao Xu,Cheng Cheng,Songmin Shang,Wei Gao,Ping Zeng,Shouxiang Jiang
Conventionally, surface-enhanced Raman spectroscopy (SERS)-active materials mainly include nanosized noble metals, semiconductors, or the complex of both, most of which are limited in practical applications because of their symbiotic materials, complex and difficult to control fabrication processes, and reuse and sampling challenges. To address these issues, novel SERS substrates have been developed in this study by anchoring zeolitic imidazolate framework-67 (ZIF-67) and derivatives of ZIF-67 to cotton fabric. The designed SERS substrates show extraordinary flexibility, an excellent enhancement factor, and reusable performance. By adjusting the lowest unoccupied molecular orbital and highest occupied molecular orbital of ZIF-67 through a doping process with different metal ions, the substrates exhibit a high enhancement factor of 6.07 × 106 and a low limit of detection of 10–8 M, as well as reusability resulting from photocatalysis. The enhancement process is studied based on charge transfer resonance, interband transition resonance, ground state charge transfer, and the light coupling effect. The results contribute to the approaches in designing SERS substrates by using ZIFs as unique SERS-active materials, and provide new insights into the development of novel SERS-active materials, along with promoting the use of SERS detection in the real world by improving the flexibility of substrates.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2020
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IF 7.1
Congming Sun,Weiru Dong,Junxiang Peng,Xiaoliang Wan,Zhenli Sun,Dehao Li,Suhua Wang
Increasingly serious uranium pollution necessitates a new method for rapid qualitative and quantitative uranium detection. Some methods based on fluorescence spectroscopy and surface-enhanced Raman scattering (SERS) have been reported to detect uranyl ions (UO 2 2+ ), the main species of uranium. However, various factors affect the fluorescence selectivity and SERS quantitative analysis. Herein we report on a composite material for a dual-mode fluorescence–SERS detection protocol for simultaneous quantitative and qualitative analysis of UO 2 2+ , based on a layer-by-layer assembly strategy, combining the advantages of the two complementary techniques. The composite material consists of a Fe 3 O 4 @SiO 2 core coated by gold nanoparticles (FA) via electrostatic adsorption. The CdTe quantum dots (CdTe QDs) outside the FA are linked with amide bonds to form a satellite Fe 3 O 4 -Au@CdTe nanostructure (FA-CdTe). The fluorescence signal of FA-CdTe was selectively quenched by UO 2 2+ , with a detection limit as low as 1.2 nM; the material was also used as a SERS substrate to detect the Raman mode of uranyl at 828 cm −1 . Furthermore, this method has been applied for UO 2 2+ detection in seawater, river water, and tap water, demonstrating the broad application prospects and providing novel development concepts.
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MICROCHEMICAL JOURNAL
·
2020
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IF 3.594
Zhengdong Shen,Haiying Wang,Qian Yu,Qiang Li,Xiaomin Lu,Xianming Kong
The accumulated polycyclic aromatic hydrocarbons (PAHs) in edible oil can bring serious health concerns because of their carcinogenic features. PAHs could accumulate in edible oil from contaminated environment or food stuff. In this study, the combination uses of on-site thin layer chromatography and surface enhanced Raman scattering (SERS) spectroscopy has been successfully displayed to identify PAHs from edible oil samples. Diatomite which is a kind of porous biosilica was used to construct stationary phase on TLC plate, the photonic crystal feature of diatomite is beneficial for SERS enhancement. The highly-porous stationary phase composed of porous diatomite with void shell structure could promote mobile phase interaction with the stationary phase, which leading to improved mass transfer, uniformity and analyte resolution compare with the conventional silica gel plate. The edible oil (1 µL) was directly applied to the diatomite plate without pretreatment, and subjected to TLC separation. The polarity of target analytes was calculated for eluent optimization, on which four compounds of PAHs(benzo[a]pyrene, pyrene, anthracene and indeno[1,2,3-cd] pyrene) were successfully separated by using hexane/chloroform (15/1, v/v) as eluent, including compounds with similar polarity(benzo[a]pyrene 0.0477, indeno[1,2,3-cd] pyrene 0.6545, pyrene and anthracene are 0). 2 µL of Au colloid at 2.4 × 10 −7 M were transferred onto the diatomite plate for the SERS measurement. The limit of detection was found to be nearly at 1 ppm. The obtained results can easily identify the four compounds of PAHs in edible oil by their own characteristic Raman peaks. This proposed method provides a simple, instant and effective strategy for on-site identification PAHs from food samples.
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Nano-Micro Letters
·
2021
·Vol. 13
·期 1
·1-17
·
IF 16.419
Peng Yusi,Lin Chenglong,Long Li,Masaki Tanemura,Tang Mao,Yang Lili,Liu Jianjun,Huang Zhengren,Li Zhiyuan,Luo Xiaoying,Lombardi John R.,Yang Yong
Nb 2 C and Ta 2 C MXenes exhibit remarkable SERS performance with the enhancement factors of 3.0 × 10 6 and 1.4 × 10 6 , which is synergistically enabled by the PICT resonance enhancement and electromagnetic enhancement.
The excellent SERS sensitivity endows Ta 2 C MXene with the capability to sensitively detect and accurately identify the SARS-CoV-2 spike protein, which is beneficial to achieve real-time monitoring and early warning of novel coronavirus.
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JOURNAL OF HAZARDOUS MATERIALS
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2020
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IF 9.038
Jiajia Shan,Yituo Zhang,Jian Wang,Tao Ren,Mengke Jin,Xue Wang
Microplastics (MPs) have been proven to concentrate hydrophobic organic contaminants (HOCs) from seawater as the sorbent phase, and the concentration of HOCs in aqueous solutions could be estimated from MPs preloaded with HOCs by equilibrium partition coefficient. This study firstly proposed to in situ quantify fluoranthene (a representative HOCs) pre-concentrated on MPs using surface enhance raman scattering (SERS) in combination with mathematical models, as an efficient monitoring tool for fluoranthene pollution in the aquatic environment. AgNPs-coated quartz (AgNPs@SiO2) substrate was fabricated. The SERS substrate was tested using fluoranthene standard solution with the minimal detectable concentration of 1 ng/mL achieved. Applying SERS for the detection of fluoranthene sorbed on MPs, the detection limit of fluoranthene on MPs was 3.3 ng/g, where the concentration in the corresponding equilibrium seawater was 0.97 ng/mL. Since more than one fluoranthene peak was observed, the quantitative detection was investigated by interval partial least square model. Eight characteristic peak ranges were selected to develop the model for predicting fluoranthene concentration, with R 2 c and R 2 v of 0.90 and 0.82, respectively. The study provides a promising solution to monitor trace level of contaminations in aquatic environment, using MPs as the passive sampler.
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ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Huadong Zhang,Huasheng Lai,Xiangrong Wu,Gongke Li,Yufei Hu
Fast and accurate practical sample detection is a great challenge in on-site detection. Herein, we developed a CoFe2O4@HNTs/AuNPs substrate for rapid and efficient magnetic solid-phase extraction (MSPE) surface-enhanced Raman scattering (SERS) detection of aromatic amines and nitrofuran in real samples all-in-one. Magnetic CoFe2O4 beads filled inside halloysite nanotubes (HNTs) can avoid aggregation of particles, endow the substrate with the rapid magnetic separation ability to simplify the pretreatment procedure, and reduce complex matrix interference. Meanwhile, outer surface AuNPs can generate electromagnetic enhancement and hot spots to amplify Raman signals of target molecules enriched/concentrated by HNTs. The CoFe2O4@HNTs/AuNPs substrate exhibited excellent SERS activity (high sensitivity, good reproducibility, and repeatability), pH stability (3.0–11.0), and good MSPE ability (fast magnetic enrichment/separation ability within 5 min). The CoFe2O4@HNTs/AuNPs MSPE SERS substrate can be applied for the determination of 4,4′-thioaniline and nitrofurantoin with a linear range of 0.054–21.7 mg/L and 0.05–1.0 mg/L, and the limits of detection were down to 0.026 mg/L and 0.014 mg/L, respectively. Furthermore, the enhancement factor (EF) of the substrate to 4,4′-thioaniline is up to 2.7 × 107. Besides, the substrate can realize practical SERS determination of trace 4,4-thioaniline in cosmetics and nitrofurantoin in fish feed and aquatic samples. The recoveries were varied from 71.6% to 103.6% for 4,4-thioaniline in hair dyes and 81.9% to 116.3% for nitrofurantoin in fish feed and aquatic samples, respectively. Such a robust and efficient MSPE SERS substrate possesses great potential in rapid detection (within 15 min) for a practical sample, and it also provides a methodology for the preparation of other HNTs-based composites.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2020
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IF 3.232
Nisar Hussain,Hongbin Pu,Abid Hussain,Da-Wen Sun
Existing approaches for the screening of unsafe materials in food matrices are time-consuming, tiresome and destructive in nature. Therefore, in the current study, a surface-enhanced Raman spectroscopy (SERS) method based on octanethiol-functionalized core-shell nanoparticles (Oct/Au@AgNPs) was established for rapid detection of ziram in apple and pear fruits. The morphology of substrate was evaluated using high-resolution TEM images and superimposed HAADF-STEM-EDS elemental mapping images, which confirmed that Au@AgNPs having gold (Au) core size of 28 nm in diameter and silver (Ag) shell of 5.5 nm in thickness were successfully grafted with octanethiol. The SERS method with the sensitive nanoparticles could detect ziram of up to 0.015 and 0.016 ppm in apple and pear with high coefficients of determination (R 2 ) of 0.9987 and 0.9993, respectively. Furthermore, satisfactory recoveries (80–106%) were also accomplished for the fungicide in real samples. This work demonstrated that the functionalized silver-coated gold nanoparticles were easy to prepare and could be used as sensitive SERS platforms for monitoring of other agrochemicals in foods.
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JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
·
2021
·Vol. 99
·期 3
·614-626
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IF 2.326
Xiao Yuwei,Wang Chaoyang,Liu Kui,Wei Lingyu,Luo Zhihui,Zeng Min,Yi Yong
In this work, a new preparation method of highly pure gold aerogel using cellulose triacetate as a template was developed. Gold aerogel with high-purity, low density, and elevated specific surface area was successfully prepared by the proposed fast and simple method as a typical example of noble metal aerogels. In this process, the ligament, crystal grain size, and specific surface area of the product could be controlled by adjusting the concentration of the reducing agent. After complete removal of the template, the obtained gold aerogel with 99% purity showed a moderate catalytic effect toward organic pollutants but with great modification potential. The Raman intensity of pure gold aerogel was 3.2-fold higher than that of the conventional porous gold substrate. The excellent effect of the as-prepared nanogold aerogel was attributed to its enhanced surface plasmon resonance effect induced from high purity. In sum, these findings look promising for future application of high-purity gold aerogels in many fields.
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TALANTA
·
2021
·
IF 6.057
Yuanyuan Yao,Hongding Zhang,Tongtong Tian,Yixin Liu,Rendan Zhu,Ji Ji,Baohong Liu
With the emergence of microRNA (miRNA) as a key player in early clinical disease diagnosis, development of rapidly sensitive and quantitative miRNA detection methods are imperative. Herein, a label-free SERS assay coupled with duplex-specific nuclease (DSN) signal amplification strategy was proposed for facilely ultrasensitive and quantitative analysis of miRNA-21. Firstly, magnetic beads assembled with excessive capture DNA were utilized to hybridize the target miRNA-21. These DNA-RNA heteroduplexes were cleaved by DSN to generate small nucleotide fragments into the supernatant and the miRNA-21 released and rehybridized another DNA, going to the next DSN cycle. Consequently, numerous of small nucleotide fragments of capture DNA were released from magnetic beads and the miRNA-21 signal was transferred and amplified by the SERS signals of total phosphate backbones which are abundant in nucleotide. Furthermore, iodide-modified Ag nanoparticles (AgINPs) was employed to generate a strong and reproducible SERS signal. The proposed method displayed excellent performance for miRNA-21 detection with the linear range from 0.33 fM to 3.3 pM, and a lower detection limit of 42 aM. Moreover, this strategy exhibited effectively base discrimination capability and was successfully applied for monitoring the expression levels of miRNA-21 in different cancer cell lines and human serum.
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OPTICAL MATERIALS
·
2021
·
IF 3.08
Guoqi Sui,Xiuyun Yang,Hongji Li,Yunhui Li,Leijiao Li,Jing Shao,Yi Li,Junfu Wang,Yong Xue,Jinyue Zhang,Shuo Dong
A novel method for the detection of antibiotics is developed by combining surface enhanced Raman scattering (SERS) with molecularly imprinted polymers (MIPs) on membrane materials. In this test, eggshell membrane (ESM) is selected as support material, which shows the advantages of low price, easy preparation and more functional groups comparing with other common synthetic membranes. Firstly, Ag nanoparticles with different morphologies are deposited on the surface of ESM by in-situ reduction, and then imprinted polymer is coated by precipitation polymerization to promote high specific sensitivity. The results present that the sensitivity of SERS imprinted membrane is mainly affected by Ag morphology. Under the optimal conditions, it presents a good linear relationship between SERS intensity and spiramycin (SP) concentration, and the coefficient of determination (R 2 ) is 0.9981. It also exhibits the ability of selective detection and retrievability. It can be recycled at least 10 times. This method provides a cheap, stable and sensitive substrate, which broadens the research field of SERS detection of antibiotics.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 19
·4775-4784
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IF 4.157
Gao Ningning,Wang Qing,Tang Jun,Yao Shengyuan,Li Hongmei,Yue Xiaxia,Fu Jihong,Zhong Furu,Wang Tao,Wang Jing
Surface-enhanced Raman scattering (SERS), as a rapid, reliable and non-destructive spectral detection technology, has made a series of breakthrough achievements in screening and pre-diagnosis of various cancerous tumors. In this paper, high-performance gold nanoparticles/785 porous silicon photonic crystals (Au NPs/785 PSi PhCs) active SERS substrates were specially designed for serum testing, and realized highly sensitive detection of serum from healthy people, patients with cervical cancer and breast cancer. Based on the SERS spectra of the three groups of serum, the significant differences between the healthy group and cancer group at 1030 cm −1 and 1051 cm −1 were analyzed, and the similar but different serum SERS spectra of cervical cancer and breast cancer patients were compared. In addition, the spectral difference detected by SERS technology combined with a multivariate statistical algorithm was used to distinguish three kinds of serum. The serum SERS spectral sensitive bands were extracted by recursive weighted partial least squares (rPLS), and the three classification diagnosis models were established by combining orthogonal partial least squares discriminant analysis (OPLS-DA), linear discriminant analysis (LDA) and principal component analysis support vector machine (PCA-SVM) for synchronous classification and discrimination of the three groups of serum. The diagnostic results showed that the overall screening accuracy of three models were 93.28%, 97.77% and 94.78%, respectively. These above results confirmed that the Au NPs/785 PSi PhCs can realize super-sensitive detection of serum, and the established diagnostic model has great potential for pre-diagnosis and simultaneous screening of cervical cancer and breast cancer. Graphical abstract
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危险品化学品经营许可证（带存储）