Nature Communications
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2025
·Vol. 16
·期 1
·1-19
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IF 15.7
Qu Ying,Chen Chen,Sun Feifei,Liu Shijia,Zhao Guozhi,Zhao Zhongxi,Liu Chang,Jiang Xinyi,Li Tao
Epi-immunotherapy appears promising for hepatocellular carcinoma (HCC) treatment, but immunosuppressive macrophages limit the capacity of epigenetic regulation to activate T cell-mediated tumoricidal immunity. Here we report an epi-immune nanosatellite (stEiNS) that co-delivers siRNA targeting the YTH N6-methyladenosine RNA binding protein 1 (YTHDF1) alongside the histone deacetylase IIa inhibitor TMP195, enabling epigenetic reprogramming of HCC tumor cells and M2 macrophages to enhance the immunotherapeutic response. stEiNS assembles size-mismatched nanoparticles via dynamic locks in a satellite-like structure, enabling deep tissue penetration. Knockdown of YTHDF1 by stEiNS in HCC cells, along with stEiNS-driven antitumor macrophage phenotype induction, intensifies macrophages-cytotoxic T lymphocytes interactions with tumor cells. stEiNS suppresses TNF/NF-κB signaling in tumor cells to inhibit CCL2-driven recruitment of myeloid-derived suppressor cells while activating the IFNγ/STAT1 pathway in M2-phenotype macrophages to promote their polarization toward an M1 phenotype. Collectively, these effects trigger robust tumoricidal immunity, leading to efficient tumor eradication, as validated in patient-derived tumor organoids, orthotopic HCC models, and recurrence models. In summary, we establish a dual-targeting stEiNS with promising epi-immunotherapeutic potential against advanced HCC and diverse malignancies.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-9
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IF 15.7
Jia Ming-Yang,Zhou Yue-Wen,Yang Jun-Lei,Liu Qinlei,Cai Zhen-Feng
Ullmann-type coupling reactions are one of the most important transformations in organic synthesis. Achieving catalyst-free Ullmann couplings under mild conditions is crucial to extending the scope of traditional carbon-carbon and carbon-heteroatom couplings. Herein, we show that Ullmann couplings can be effectively achieved at room temperature without the need for metal catalysts in MeOH/H 2 O microdroplets. Mechanistic investigations reveal that the Ullmann reaction in microdroplets is driven by •OH radicals and involves a single-electron transfer pathway via nitrogen-centered radicals. This work not only provides fundamental insights into the •OH radical-mediated coupling reactions in microdroplets but also offers a new strategy for catalyst-free Ullmann couplings.
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JOURNAL OF MATERIALS SCIENCE
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2025
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IF 3.9
Wang Kexin,Li Mingyang,Geng Yiming,Fu Xiao,Chen Zhanwei
Chronic non-healing wounds typically arise from bacterial adhesion and colonization. Plumbagin (PLB), a secondary metabolite derived from Droseraceae , Plumbaginaceae , and Ebenceae, exhibits potent antibacterial activity. In this study, zeolite imidazole framework-8 (ZIF-8) was utilized to encapsulate Plumbagin, constructing the nanoparticle PLB@ZIF. In both in vitro antibacterial experiments and in vivo wound healing assays , PLB@ZIF demonstrated effective synergistic therapy against methicillin-resistant S. aureus (MRSA) and Escherichia coli ( E. coli ). In addition, compared to PLB or ZIF-8 alone, PLB@ZIF exhibited superior antibacterial efficacy. These results suggested that ZIF-8 significantly enhances the antibacterial efficiency of Plumbagin in wound healing, highlighting the broad application potential of PLB@ZIF for treating wound infection caused by MRSA and E. coli .
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Scientific Reports
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2025
·Vol. 15
·期 1
·1-11
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IF 3.9
Yu Xiang-Fang,Gurung Chetali,Yu Zhongjia,Xiao Wei,Kerem Goher,Teng Bin,Zhang Jian V.,Su Zhe,Zhou Yongjin,Ren Pei-Gen
Awareness of the intestinal microflora involved in insulin resistance and type 2 diabetes (T2DM) has now become more evident. However, direct mechanical insight is required to illustrate the contribution of intestinal microflora in the disease progression. In this study, we aimed to precisely assess the changes in intestinal bacteria translocation (IBT) from gut to the pancreas using combinations of FISH, 16S rRNA amplicon sequencing, and deep learning-assisted methods to track IBT in diet-induced obese (DIO) and antibiotic-induced microbiota disruption (AIMD)-DIO mouse models. Our analysis showed deep learning-assisted quantification enhanced the accuracy and objectivity of bacterial tracking. The DIO mice exhibited increased IBT, likely due to excessive intestinal lipid accumulation and compromised intestinal barrier integrity. Elevated bacterial loads in the pancreas were associated with worsened pancreatic function, indicated by higher fasting blood glucose, impaired glucose tolerance, and dysfunctional insulin secretion. In contrast, the AIMD-DIO group lowered the IBT, maintained the islet structure and improved glucose homeostasis. Comparative study between DIO and AIMD-DIO models revealed a strong correlation between number of translocated bacteria and T2DM severity. These findings provide objective evidence of bacterial migration from the intestine to the pancreas and establishes its pathological relationship with pancreatic impairment and dysfunction and underscores to utilize AI techniques more successfully in the future for evaluating IBT.
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JOURNAL OF IRON AND STEEL RESEARCH INTERNATIONAL
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2025
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IF 3.6
Jin Hu-lin,Ma Yan,Wang Zhou-fu,Liu Hao,Dong Yun-jie,Yi Nian,Wang Xi-tang,Deng Cheng-ji,Xia Zhong-feng,Quan Zheng-huang,Zhang Ling,Tong Guang-da,Fu Wei-dong
MgO–CaO refractories have attracted much attention in the field of clean steel due to the ability of their internal CaO to adsorb elements such as S and P in molten steel. However, there are still some problems such as difficult sintering and insufficient corrosion resistance existing in this system. Different contents of Y 2 O 3 were introduced into MgO–CaO system to prepare MgO–CaO–Y 2 O 3 ternary refractories via traditional and induction sintering methods. The influence of microstructural regulation on the slag-resistant properties of the refractories was investigated. The results show that the introduction of Y 2 O 3 in the MgO–CaO refractories prepared via the two sintering methods leads to the grain boundary reconstruction effect. Under the condition of traditional sintering, when a smaller amount of Y 2 O 3 is introduced into the MgO–CaO refractories, Y 2 O 3 is able to activate the lattice, promote sintering, and improve the densification of the refractories. However, when more Y 2 O 3 is introduced, the excess Y 2 O 3 hinders the sintering densification process. Combined with lamellar intergranular phase generated in the refractories, Y 2 O 3 -based solid solution can react with the slag, increase the slag viscosity and inhibit the penetration of the slag into the refractories. Under the condition of induction sintering, the solid solution of yttrium ions in CaO is increased by using the coupling of electromagnetic and thermal fields. Compared with the MgO–CaO refractories with high Y 2 O 3 content prepared by traditional sintering, the induction sintered refractories have higher densification, which further increases the corrosion resistance. The results provide a new path for developing long-life MgO–CaO based refractories.
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BIOLOGICAL TRACE ELEMENT RESEARCH
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2025
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IF 3.6
Liu Kan,Tao Tao,Li Ranhong,Wang Huanping,Wang Li,Wu Haiying
Arsenic exposure is well-established to cause neurotoxicity, but its underlying molecular mechanisms remain unclear. This study investigates the effects of early-life arsenic exposure on the nervous system of offspring. Female mice were exposed to NaAsO₂, and alterations in learning, memory, and hippocampal lesions in offspring were assessed. Transcriptome analysis, protein interaction studies, and cellular experiments were integrated to identify key regulatory factors in arsenic-induced neurotoxicity. Arsenic interacts with HUWE1, significantly downregulating its protein expression. HUWE1 inhibition enhances GPX8 stability by reducing its ubiquitination. GPX8 disrupts histone epigenetic modifications by promoting GSH synthesis and arsenic methylation, leading to elevated H3K9 levels. HUWE1 also interacts with KDM3A, and its inhibition reduces KDM3A-mediated H3K9me1/me2 demethylation, amplifying H3K9me2-mediated transcriptional repression of LARS2 and triggering PANoptosis. This study provides novel molecular insights into arsenic-induced neurotoxicity and highlights potential therapeutic targets to mitigate arsenic-related nervous system damage.
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BMC BIOTECHNOLOGY
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2025
·Vol. 25
·期 1
·1-20
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IF 3.4
Long Shengyu,Wang Wentong,Chen Yongcheng,Wang Zhihua,Duan Hao,Yuan Ping,Xu Yunrong,Li Denghui,Zhang Wan,Wang Weizhou,He Fei
Background The repair of bone defects remains a significant clinical challenge. Although magnesium (Mg)-based biomimetic scaffolds are widely utilized for bone defect repair, the release of Mg²⁺ ions often leads to an alkaline microenvironment, thereby adversely affecting bone regeneration. Regenerative medicine strategies that leverage the recruitment of endogenous bone marrow mesenchymal stem cells (BMSCs) offer a novel approach to treating bone defects.Methods In this study, we employed poly(L-lactic acid) (PLLA) and polyethylene glycol (PEG) as shell materials and nanomagnesium oxide (nMgO) combined with gelatin (G) as core materials to fabricate coaxial fibre membranes with a “core‒shell” structure via coaxial electrospinning technology. Additionally, we grafted the BMSC-affinitive peptide E7 (EPLQLKM) onto the fibres to achieve specific recruitment of endogenous BMSCs.Results Morphological and structural analyses confirmed the successful formation of the “core‒shell” structure of the fibre membranes. Grafting E7 peptides enhanced the hydrophilicity and mechanical properties of the fibre membranes and maintained pH stability in vitro. In vitro experiments demonstrated that the functionalized fibre membranes significantly promoted BMSC proliferation, migration, and osteogenic differentiation. When implanted into a rat cranial defect model, we observed the formation of new bone tissue and the repair of the bone defect.Conclusion sE7 peptide-functionalized coaxial fibre membranes effectively facilitated bone defect repair by promoting the recruitment and osteogenic differentiation of BMSCs, demonstrating substantial potential for tissue engineering applications.
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CLINICAL & EXPERIMENTAL METASTASIS
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2025
·Vol. 42
·期 5
·1-20
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IF 3.2
Liu Jinren,Wang Xiangni,Cui Yixin,Lu Jiajia,He Zhirou,Xu Yulin,Li Rongrong,Xu Guimin,Gao Lingge,Fan Xiaolin,Wu Xili,Shi Xingmin,Zhang Guanjun
Ovarian cancer remains a significant challenge in oncology due to its aggressive nature, late-stage diagnosis, and high rates of chemoresistance, particularly to platinum-based therapies like cisplatin. The epithelial-mesenchymal transition (EMT) is a key driver of ovarian cancer metastasis and drug resistance, highlighting the need for novel therapeutic strategies. Cold atmospheric plasma (CAP) and plasma-activated liquids (PAL), including plasma-activated medium (PAM) and saline (PAS), have emerged as promising anticancer agents, generating reactive oxygen and nitrogen species (RONS) that selectively target cancer cells. This study investigates the potential of PAL to inhibit the invasion and metastasis of cisplatin-resistant ovarian cancer cells and explores its synergistic effects with cisplatin. In vitro , PAM reduced proliferation, migration, and invasion of cisplatin-resistant ovarian cancer cells (A2780/DDP and SKOV3/DDP) while downregulating EMT-related proteins (N-cadherin, β-catenin, vimentin). H 2 O 2 in PAM inhibit the PI3K/AKT/GSK3β pathway, promoting degradation of EMT regulators Snail, Slug, and β-catenin. Combining PAM with cisplatin enhanced therapeutic efficacy, reducing cell viability and metastatic potential. In vivo studies using an orthotopic mouse model further confirmed that PAS combined with low-dose cisplatin effectively suppressed tumor growth and metastasis with minimal side effects. These findings underscore the potential of PAL as an adjuvant therapy for cisplatin-resistant ovarian cancer, offering a novel approach to overcome drug resistance and inhibit metastasis. Future research should focus on optimizing treatment protocols and elucidating the molecular mechanisms underlying the synergistic effects of PAL and cisplatin.
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WATER AIR AND SOIL POLLUTION
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2025
·Vol. 236
·期 12
·1-16
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IF 3
Liu Yucan,Wang Baoyan,Hu Jing,Sun Hongwei,Lv Xintao,Qu Zhiming,Yang Xiaoyong,Wang Gang,Zhang Yanxiang,Zhang Shujun
Per– and polyfluoroalkyl substances (PFAS), especially perfluorobutane sulfonate (PFBS), are persistent organic pollutants with significant ecological risks. This study investigates the effect of PFBS on the properties of coastal sediment, including physicochemical characteristics microbial enzymatic activities, and community structure, in a tidal marsh on the Shandong Peninsula, China. 0, 0.1, 10, and 1000 µg/g PFBS were added to the sediments and incubated at 20 °C for six weeks. To evaluate changes in total organic carbon (TOC), total nitrogen (TN), microbial metabolites, enzyme activities, and community diversity. The results showed that PFBS concentration has a strong effect on TOC accumulation, pore water TOC decreased to 0.69 times the control level at 0.1 µg/g PFBS ( p < 0.05) but increased sharply 2.95 times the control (37.74 ± 3.31 mg/L) at 1000 µg/g PFBS. Enzymatic stress response, with urease and phosphatase activities increased 2.91 times and 2.95 times, respectively, at the highest PFBS concentration (1000 µg/g, p < 0.05), suggesting microbial compensation mechanisms. Furthermore, PFBS significantly altered microbial community composition, reducing the relative abundance of Nitrososphaerota (a key nitrifying phylum) by 76% at 1000 µg/g PFBS. These results highlight the potential of PFBS to disrupt microbial dynamics and nutrient cycling in coastal sediments, and underscoring the importance of monitoring short‒chain perfluorinated compounds (PFCs) in the environments due to their potential long‒term ecological risks.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
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2025
·Vol. 36
·期 24
·1-20
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IF 2.8
Li Yuzhen,Li Shuo,Xia Yunsheng,Sun Zhaoxin,Lan Borong
Photocatalytic technology had attracted attention for the abatement of organic contaminants in wastewater with solar energy. In this study, a binary supported heterojunction photocatalyst (AgIn(MoO 4 ) 2 /g-C 3 N 4 ) was constructed by a facile impregnation method, and the catalyst was utilized for the efficient degradation of tetracycline (TC) under visible light. The structure, micro-morphology and optical properties of the photocatalyst were also characterized and analyzed. Compared with g-C 3 N 4 and AgIn(MoO 4 ) 2 , the composites exhibited excellent photocatalytic performance. Among them, superoxide radicals, hydroxyl radicals and holes were participated in the degradation reaction. In addition, the composites had good cyclic stability and a broad pH usage range. The enhanced photocatalytic degradation performance could be attributed to the Z-scheme electron transfer mechanism, which both promoted the separation of photogenerated carriers and utilized the strongest redox capacity in the monomer. Overall, this work provided a feasible strategy for constructing g-C 3 N 4 -based heterojunction catalysts for efficient removal of TC from polluted water.
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JOURNAL OF POLYMER RESEARCH
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2025
·Vol. 32
·期 9
·1-17
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IF 2.8
Zhang Rui,Ma Haihong,Zhou Zhengfa,Xu Weibing,Ren Fengmei
A modified silicone oil containing nitrogen, phosphorus, and sulfur was synthesized by the reaction of n-butyl thiophosphoric triamine (NBPT) and epoxidized silicone oil (ETSO), and named NBSO. The successful synthesis of NBSO was confirmed by Fourier infrared spectroscopy (FT-IR) and nuclear magnetic resonance spectroscopy (NMR). The flame retardant and smoke suppression properties of NBSO were investigated by open flame ignition method and cone calorimetry test (CCT). The results showed when the molar ratio of NBPT to ETSO was 1:1, the flame height of the modified silicone oil was significantly reduced, and compared with ETSO, the total smoke release (TSR) and the total heat release (THR) of NBSO was reduced by 67.6% and 68%, respectively. Thermogravimetric analysis (TGA), infrared thermogravimetric coupling (TG-IR), thermal field emission scanning electron microscopy (SEM), and X-ray photoelectron spectroscopy (XPS) techniques were used to analyze the mechanism of flame retardant and smoke inhibition. The results indicate that compared with ETSO. NBSO has a slower thermal decomposition rate and releases less flammable gas.In the condense phase, nitrogen, phosphorus, silicon, and sulfur could improve the carbon forming ability through synergistic effect. At the same time, a silicon oxide layer was formed on the outer surface of the carbon layer, which could increase the flame retardation and smoke suppressing effect.
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Environmental Earth Sciences
·
2025
·Vol. 84
·期 16
·1-13
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IF 2.8
Cao Qing,You Bensheng,Xu Haibo,Liu Weijing,Ma Shuzhan
Copper (Cu) and cadmium (Cd) are commonly found in polluted water bodies due to intensive anthropogenic activities, but their combined toxicity on Microcystis aeruginosa remains unknown. The present study conducted a 96-h cultivation experiment to investigate the toxicity effects of Cu and Cd, both individually and in mixture, on the growth, oxidative response and gene expression of Microcystis aeruginosa . Results showed that Cd alone had no negative impact on M. aeruginosa growth; whereas growth was significantly inhibited by Cu alone and by the mixture. High concentrations of Cu (200 µg L − 1 ) and Cd (50 µg L − 1 ), as well as their mixture, induced oxidative stress in M. aeruginosa . The ftsH gene, prx gene, fabZ gene and recA gene were all up-regulated by high concentrations of Cu (200 µg L − 1 ), Cd (50 µg L − 1 ) and their mixtures (100 + 25 and 200 + 50 µg L − 1 ), which however, decreased the expression of the psbA gene. The combined toxicity assessment suggested that synergistic interaction occurred in treatments with low Cu and Cd concentrations (100 + 25 µg L − 1 ). Our results suggest that the toxicity effects of Cu and Cd are exacerbated when they co-exist in the natural environment at relatively low concentrations.
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