LWT-FOOD SCIENCE AND TECHNOLOGY
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2024
·
IF 6
Huijuan Liu,Dong Xiang,Zhifei Cheng,Lin Ma,Shuyu Yang,Jiao Xie
Fresh D . rubrovolvata and P . impudicus are perishable due to their high water content and strong postharvest respiration, which seriously affect their edible value and economic benefits. Drying performs the crucial role in maintaining the quality of edible fungi and extending their shelf life, while the extraction method after drying is also important to the material composition and functional properties of edible fungi. According to the color difference and texture indicators, 45 °C was found to be the optimal temperature choice for both hot-air and vacuum drying. In addition, polysaccharide content in the extract of dried D . rubrovolvata was higher than that of P . impudicus, while the polyphenol abundance in the extract of dried P . impudicus was better than that of D . rubrovolvata. Hot water extraction or thermal ultrasonic treatment was beneficial for the antioxidant activity in D . rubrovolvata and P . impudicus extracts. Based on the correlation analysis, it was showed that polyphenols contributed significantly more to the antioxidant capacity of D . rubrovolvata and P . impudicus, especially P . impudicus, than that of polysaccharides. The related results provided new insights into the improvement of product quality and the development of functional products for D . rubrovolvata and P . impudicus.
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Frontiers in Bioengineering and Biotechnology
·
2024
·
IF 4.3
Lei Han,Chaoqian Zhao,Yufang Zhu,Huang Li
Introduction: Bone tissue engineering is considered the ideal approach for bone repair. Mesoporous bioactive glass (MBG) possesses the characteristics of high drug-loading capacity and bioactivity. Low-intensity pulsed ultrasound contributes to promoting fracture healing and bone defect repair, and dimethyloxalyl glycine (DMOG) is a small molecular inhibitor that can suppress prolyl hydroxylase, reducing the degradation of hypoxia-inducible factor. Methods: In this study, we proposed to prepare DMOG-loaded MBG/poly(D,L-lactide) composite scaffolds (DMOG-MBG/PDLLA) for promoting bone repair. The effects of ultrasound stimulation and DMOG release on the cell responses of rat bone marrow mesenchymal stem cells (BMSCs) and human umbilical vein endothelial cells (HUVECs) and bone repair in vivo were investigated. Results and Discussion: The results showed that both ultrasound stimulation and DMOG release could promote the proliferation, adhesion and differentiation of BMSCs and HUVECs, respectively. After the implantation of scaffolds in rat cranial bone defect model for 8 weeks, the results indicated that the combined ultrasound stimulation and DMOG release contributed to the highest ability for promoting bone repair. Hence, the DMOG-MBG/PDLLA scaffolds with ultrasound stimulation are promising for application in bone repair.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Jiying Zhu,Wei Jia,Jian Peng
Crocus sativus L. is a both medicinal and food bulbous flower whose qualities are geographically characterized. However, identification involving different places of origin of such substances is currently limited to single-omics mediated content analysis. Integrated metabolomics and proteomics, 840 saffron samples from six countries (Spain, Greece, Iran, China, Japan, and India) were analyzed using the QuEChERS extraction method. A total of 77 differential metabolites and 14 differential proteins were identified. The limits of detection of the method were 1.33 to 8.33 μg kg −1 , and the recoveries were 85.56% to 105.18%. Using homology modeling and molecular docking, the Gln84, Lys195, Val182 and Pro184 sites of Crocetin glucosyltransferase 2 were found to be the targets of crocetin binding. By multivariate statistical analysis (PCA and PLS-DA), different saffron samples were clearly distinguished. The results provided the basis for the selection and identification of high quality saffron from different producing areas.
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Atmosphere
·
2024
·Vol. 15
·期 7
·825
·
IF 2.5
Chuang Li,Yue Li,Yingsheng Liu,Shanshan Zhong,Huanshi Zhang,Zhelun Xu,Zhongyi Xu,Daolin Du,Congyan Wang
One of the key reasons for the success of invasive plants is the functional differences between invasive plants and native plants. However, atmospheric nitrogen deposition may disrupt the level of available nitrogen in soil and the functional differences between invasive plants and native plants, which may alter the colonization of invasive plants. Thus, there is a pressing necessity to examine the effects of atmospheric nitrogen deposition containing different nitrogen components on the functional differences between invasive plants and native plants. However, the progress made thus far in this field is not sufficiently detailed. This study aimed to elucidate the effects of artificially simulated nitrogen deposition containing different nitrogen components (i.e., nitrate, ammonium, urea, and mixed nitrogen) on the functional differences between the Asteraceae invasive plantBidens pilosaL. and the Asteraceae native plantPterocypsela laciniata(Houtt.) Shih. The study was conducted over a four-month period using a pot-competitive co-culture experiment. The growth performance ofP. laciniata, in particular with regard to the sunlight capture capacity (55.12% lower), plant supporting capacity (45.92% lower), leaf photosynthetic area (51.24% lower), and plant growth competitiveness (79.92% lower), may be significantly inhibited under co-cultivation condition in comparison to monoculture condition.Bidens pilosaexhibited a more pronounced competitive advantage overP. laciniata, particularly in terms of the sunlight capture capacity (129.43% higher), leaf photosynthetic capacity (40.06% higher), and enzymatic defense capacity under stress to oxidative stress (956.44% higher). The application of artificially simulated nitrogen deposition was found to facilitate the growth performance of monoculturalP. laciniata, particularly in terms of the sunlight capture capacity and leaf photosynthetic area.Bidens pilosaexhibited a more pronounced competitive advantage (the average value of the relative dominance index ofB. pilosais ≈ 0.8995) thanP. laciniataunder artificially simulated nitrogen deposition containing different nitrogen components, especially when treated with ammonium (the relative dominance index ofB. pilosais ≈ 0.9363) and mixed nitrogen (the relative dominance index ofB. pilosais ≈ 0.9328). Consequently, atmospheric nitrogen deposition, especially the increased relative proportion of ammonium in atmospheric nitrogen deposition, may facilitate the colonization ofB. pilosavia a stronger competitive advantage.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Zhijiang Sun,Dahui Wang,Nailing Sun,Fen Ran
In recent years, a range of high-performance ultrafiltration membranes have been developed, but maintaining high retention without sacrificing high throughput remains a serious challenge. In this paper, functionalized halloysite nanotubes is constructed by copolymerization and by a one-step reaction of L -arginine and dopamine hydrochloride using halloysite nanotubes as carriers. Subsequently, modified polyethersulfone membranes are prepared by blending them as additives through non-solvent-induced phase transition separation. As the synthesized nanofillers is rich in functional groups, negative electronegativity, and unique structures, the study focuses on adding nanofillers’ effect on the membrane structure and physicochemical properties. The addition of appropriate nanofillers enhances the membrane surface wettability and negative electronegativity, and the enlarged internal pores and the dense layer that remains flat and homogeneous effectively promote the flux of small molecules, the retention of large molecules, and the cyclic stability of the membrane. The prepared polyethersulfone membranes demonstrate exceptional cycle stability, high separation efficiency (almost 100 % for Congo red), and significantly improved permeation performance (initial permeate flux up to 316.5 L m -2 h −1 ). The results demonstrate the high potential of the modified prepared polyethersulfone membranes for pertinent environmental separation processes.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2024
·
IF 3.1
Wenchan Deng,Rongrong Huang,Yuanjiang Pan,Cuirong Sun
The metabolism process of amino acids is closely related to the growth of normal and cancer cells. It is still not clear how L/D-configuration amino acids participate in the metabolism of colorectal cell. Herein, intra- and extra-cellular metabolic distribution of L/D-amino acids in colorectal cell (HCT116) and human normal intestinal epithelial cell (NCM460) were profiled utilizing HPLC-MS/MS coupled with a chiral probe. The results displayed the differential metabolic portrayal for the two cell lines. Compared with NCM460 cell, 13 kinds of significant differential amino acids were founded in a lower concentration within HCT116 cell, and L-Gln was even not detected for intra-cell; as for extra-cell culture medium, the HCT116 cell consumed more L-Gln, D-Phe and D-Leu, while L-Met was low ingested in HCT116 cell. L-Ala and Gly were excretion in both two cell lines, excepted L-Cit which was uptake in HCT116 and excretion in NCM460 cell. Furthermore, the dynamic changes of chiral amino acids displayed that phenylalanine, tyrosine and tryptophan biosynthesis and arginine biosynthesis is the major pathway for intra-cellular metabolites and extra-cellular metabolites, respectively. Moreover, with additional D-amino acids in culture medium, the results exhibited that high concentration of D-amino acids have no significant effect on the proliferation of NCM460 cell, but could influence the profiling of amino acids metabolites, and further affect the proliferation of HCT116 cell. This present work enhances the understanding of these differential amino acids metabolic network and depicts a dynamic process of metabolic dysregulation of HCT116 and NCM460 cell.
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POLYMER ENGINEERING AND SCIENCE
·
2024
·
IF 3.2
Honglin Li,Wanjun Liu,Zhize Chen
An efficient and scalable method to prepare oligolactic acid stereocomplex microplates (SC-OLA-MPs) is developed. Oligolactic acid was firstly synthesized via the melt polycondensation of lactic acid. Then, the oligo ( L -lactic acid) and oligo ( D -lactic acid) were crystallized with a mixture of dichloromethane and acetone, and consequently the stereocomplex microparticles were obtained in the form of circular microplates with a diameter of approximately 2 μm. As an eco-friendly nucleating agent, SC-OLA-MPs were introduced into poly ( L -lactic acid) (PLLA) via melt blending. The nonisothermal and isothermal crystallization of the SC-OLA-MPs/PLLA composites were investigated, and the crystal morphology and heterogeneous nucleation phenomena during the isothermal crystallization process were photographed with POM. SC-OLA-MPs show excellent nucleating effects on PLLA crystallization. With the incorporation of 10% SC-OLA-MPs, the crystallization half-time of the SC-OLA-MPs/PLLA composite decreased from 7.18 to 0.45 min at 100°C, compared to the neat PLLA. Through a short-time molding procedure, the thermal deformation temperature of PLLA is improved by the addition of SC-OLA-MPs. Highlights Polylactic acid stereocomplex microplates were fabricated by solution crystallization. This method is easy and scalable. This microplate is an effective nucleating agent for PLLA.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2025
·
IF 6.1
Lei Sun,Ya Liu,Yakun Feng,Zequn Fan,Lei Jiang,Can Lu,Cuiling Jiang
The presence of biodegradable microplastics (BMPs) alongside toxic metals in soil significantly threatens plant health. Current research mainly focuses on the effects of original BMPs. In contrast, the specific impacts of ultraviolet (UV)-aged BMPs and their interaction with Cadmium (Cd) on seed germination and growth are unclear. Therefore, this study incubated aged polylactic acid (PLA) MPs through a UV irradiation aging process and used an indoor hydroponic experiment to systematically investigate the single and combined effects of Cd and BMPs (virgin and aged) on pakchoi ( Brassica chinensis L. ) germination, photosynthesis, antioxidant systems, and Cd accumulation. The results showed that after 21 days of UV irradiation (UPLA MPs), PLA MPs formed a fractured surface, demonstrating more detrimental effects on pakchoi than virgin ones. UPLA MPs and Cd alone inhibited pakchoi germination, growth, and photosynthesis, while PLA MPs alone promoted these processes. Combined treatment with Cd and UPLA MPs significantly increased oxidative damage and reduced pakchoi root length, chlorophyll, Mg, Mn, and Zn content. Under the combination of Cd and BMPs, PLA MPs could effectively alleviate the toxic effect of Cd on pakchoi. The results unraveled here emphasized that UPLA MPs, especially aged BMPs, could trigger adverse effects on agro-systems with heavy metals. Therefore, the results of this study can provide a new perspective and reference for the ecological risk evaluation of Cd and BMPs pollution in agricultural soils.
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ELECTROANALYSIS
·
2012
·Vol. 24
·期 3
·691-698
·
IF 2.872
Fangyuan Zhao,Le Wang,Yanling Liu,Ge Song,Fei Wang,Baoxian Ye
A new voltammetric sensor for matrine was fabricated via the polymerization of L -cysteine on graphene oxide, which served as templates. The fabricated electrode can improve the oxidation of matrine intensively, and enable the determination of matrine in the range from 4×10 −6 to 1×10 −4 mol L −1 , with a detection limit of 2×10 −6 mol L −1 . The oxidation process of matrine was studied and some dynamic parameters were calculated using various electrochemical techniques. This present sensor displayed good stability, reproducibility and sensitivity for matrine. It was successfully applied to the quantitative determination of matrine in matrine injection with satisfactory results.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2018
·
IF 2.88
Shi-Ting Zhang,Panpan Li,Caiyun Liao,Tingting Luo,Xingming Kou,Dan Xiao
A ruthenium(II) bipyridyl complex conjugated with functionalized Schiff base (RuA) has been synthesized and functioned as a luminescent probe. The luminescence of RuA was greatly quenched by Cu 2+ due to its molecular coordination with paramagnetic Cu 2+ . Subsequently, the addition of l -Histidine can turn on the luminescence of the RuA-Cu(II) ensemble, which can be attributed to the replacement of RuA in RuA-Cu(II) ensemble by l -Histidine. On the basis of the quenching and recovery of the luminescence of RuA, we proposed a rapid and highly sensitive on-off-on luminescent assay for sensing Cu 2+ and l -Histidine in aqueous solution. Under the optimal conditions, Cu 2+ and l -Histidine can be detected in the concentration range of 5 nM–9.0 μM and 50 nM–30 μM, respectively, and the corresponding detection limits were calculated to be 0.35 and 0.44 nM (S/N=3), separately. The proposed luminescent probe has been successfully utilized for the analysis of Cu 2+ and l -Histidine in real samples (drinking water and biological fluids). Furthermore, the probe revealed good photostability, low cytotoxicity and excellent permeability, making it a suitable candidate for cell imaging and labeling in vitro.
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JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Xiaoqing Wang,Shangkun Liu,Shaolei Chen,Xubin He,Wenjing Duan,Siyuan Wang,Junzi Zhao,Liangquan Zhang,Qing Chen,Chunhua Xiong
Given the necessity and urgency in removing organic pollutants such as malachite green (MG) from the environment, it is vital to screen high-capacity adsorbents using artificial neural network (ANN) methods quickly and accurately. In this study, a series of ZIF-67 were synthesized, which adsorption properties for organic pollutants, especially MG, were systematically evaluated and determined as 241.720 mg g −1 (25 ℃, 2 h). The adsorption process was more consistent with pseudo-second-order kinetics and Langmuir adsorption isotherm , which correlation coefficients were 0.995 and 0.997, respectively. The chemisorption mechanism was considered to be π-π stacking interaction between imidazole and aromatic ring. Then, a Python-based neural network model using the Limited-memory BFGS algorithm was constructed by collecting the crucial structural parameters of ZIF-67 and the experimental data of batch adsorption. The model, optimized extensively, outperformed similar Matlab-based ANN with a coefficient of determination of 0.9882 and mean square error of 0.0009 in predicting ZIF-67 adsorption of MG. Furthermore, the model demonstrated a good generalization ability in the predictive training of other organic pollutants. In brief, ANN was successfully separated from the Matlab platform, providing a robust framework for high-precision prediction of organic pollutants and guiding the synthesis of adsorbents.
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CHEMOSPHERE
·
2024
·
IF 8.1
Peicheng Huang,Dan Zou,Chunhua Dong,Chongjian Tang,Qingzhu Li,Pengwei Zhao,Ping Zhang,Qi Liao,Zhihui Yang
Selenium (Se) fortification is witnessed to simultaneously inhibit absorbing Cadmium (Cd) and Arsenic (As) by rice plants, but the mechanism is unclear. Here, the effects of Se on the root morphology, iron plaque (IP) content, soil Fe 2+ content, radial oxygen loss (ROL), and enzyme activities of the rice plants in the soil contaminated by Cd and As were intensively investigated through the hydroponic and soil experiments. Se effectively alleviated the toxic effects of Cd and As on the plants and the dry weight, root length, and root width were increased by 203.18%, 33.41%, and 52.81%, respectively. It also elucidated that ROL was one of the key factors to elevate IP formation by Se and the specific pathways of Se enhancing ROL were identified. ROL of the plants in the experiment group treated by Se was increased 36.76%, and correspondingly IP was magnified 50.37%, compared to the groups with Cd and As. It was owing to Se significantly increased the root porosity (62.11%), facilitating O 2 transport to the roots. Additionally, Se enhanced the activities of catalase (CAT) and superoxide dismutase (SOD) to promote the catalytic degradation of ROS induced by Cd and As stress. It indirectly increased O 2 release in the rhizosphere , which benefit to form more robust IP serve as stronger barrier to Cd and As. The results of our study provide a novel molecular level insight for Se promoting root IP to block Cd and As uptake by the rice plants.
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