CORROSION SCIENCE
·
2024
·
IF 7.4
Yiqian Lv,Jingmao Zhao,Baomin Fan,Hassan Murtaza,Jingbao Wang,Weixiao Jing,Liuwei Chao
Synergistical passive barrier enhancement and active self-healing has been an emerging tactic for developing prominent anti-corrosion coatings. Herein, a cerium/2-methylimidazole metal-organic framework (Ce-MOF) was that is in-situ grown on ultrathin α-ZrP nanosheets with outstanding dispersibility. The parallel arrangement of α-ZrP-based nanosheets within the epoxy matrix was driven by shear force from blade-coating technology optimizing the coating's physical barrier and self-repairing capability. This advancement may herald a new era in designing smart coatings and offering potent protection against diverse corrosion types such as filiform corrosion.
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高影响因子
Arabian Journal of Chemistry
·
2024
·
IF 5.3
Chaofeng Qin,Naidong Chen,Jiao Li
This work was aimed to investigate the α-glucosidase inhibitory potential of methanol, petroleum ether, ethyl acetate, n -butanol, and water extracts/fractions of four Dendrobium species , which are traditional medicinal plants over the centuries, and discovery some important polyphenols in terms of lowering blood sugar based our previous research. Furthermore, the enzyme kinetics, HPLC, fluorescence spectra, and molecular docking were performed to provide additional insights into the binding mechanisms between individual polyphenol and α-glucosidase. All the organic extracts/fractions showed promising results on α-glucosidase with IC 50 values of 4.23–52.53 μg/mL. The EtOAcF of D. chrysotoxum (EtOAcF-DC) possessed the lowest IC 50 value (4.23 ± 0.19 μg/mL) against α-glucosidase. And an obvious correlation existed between the total phenol contents and α-glucosidase inhition capacity. Schaftoside, isoschaftoside, 2,4,7-trihydroxy-9,10-dihydrophenanthrene (TD), dihydroresveratrol, coelonin, nudol (NU), gigantol (GT), erianin, and tristin were identified as the main polyphenol components in these four Dendrobium plants. However, only TD, NU, coelonin, and GT had significant inhibitory activity on α-glucosidase. TD, NU and GT were discovered simultaneously in the EtOAc fractions (EtOAcFs) of the four Dendrobium species and occupied higher contents than other components, thus being selected as objectives for deep research. The TD from Dendrobium species was first reported as α-glucosidase inhibitor with an IC 50 value of 52.46 ± 2.34 μg/mL, which was almost 6.5 times stronger than that of acarbose (IC 50 = 338.84 ± 18.57 μg/mL), signifying it was a potential candidate for treating diabetes. NU and GT acted as uncompetitive inhibitors against α-glucosidase, while TD and EtOAcF-DC exhibited mixed-type inhibition. The fluorescence spectra analysis demonstrated that these inhibitors could quenched the fluorescence spectrum of α-glucosidase significantly. Interestingly, the NU quenched fluorescence by an unusual static quenching method. The molecular simulation illustrated these three polyphenols interacted with α-glucosidase primarily through hydrogen bonds and van der Waals interactions, and the binding sites between polyphenols and α-glucosidase supported the obtained results of inhibition kinetics. This study revealed that the phenolic-rich extracts/fractions and polyphenol compounds from Dendrobium plants could be potential α-glucosidase inhibitors.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Fuqiang Liang,Keyu Meng,Xinran Pu,Yubo Cao,Yumeng Shi,Jiayi Shi
This study investigated the molecular mechanism underlying the binding interaction between apigenin (API) and α-glucosidase (α-glu) by a combination of experimental techniques and computational simulation strategies. The spontaneously formation of stable API-α-glu complex was mainly driven by hydrogen bonds and hydrophobic forces, leading to a static fluorescence quenching of α-glu. The binding of API induced secondary structure and conformation changes of α-glu, decreasing the surface hydrophobicity of protein. Computational simulation results demonstrated that API could bind into the active cavity of α-glu via its interaction with active residues at the binding site. The important roles of key residues responsible for the binding stability and affinity between API and α-glu were further revealed by MM/PBSA results. In addition, it can be found that the entrance of active site tended to close after API binding as a result of its interaction with gate keeping residues. Furthermore, the structural basis for the binding interaction behavior of API was revealed and visualized by weak interaction analysis. The findings of our study revealed atomic-level mechanism of the interaction between API, which might shed light on the development of better inhibitors.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Qihui Lu,Hui Liao,Zedong Jiang,Yanbing Zhu,Yijuan Han,Lijun Li,Hui Ni,Qingbiao Li
Glycosylation plays a critical role in regulating activity, stability, and correct folding of enzymes. In this study, recombinant Aspergillus niger α-L-rhamnosidase (r-Rha1) was employed to explore the impact of glycosylation in Pichia pastoris on the enzymatic properties and protein folding . β-elimination reaction and deglycosylase treatment assays demonstrated that r-Rha1 undergoes primarily N-glycosylation. The deglycosylated r-Rha1 was prepared in two ways: treating with Endoglycosidase F 1 after expression (referred to as r-Rha1- vitro ), or inhibiting intracellular glycosylation using tunicamycin (referred to as r-Rha1- vivo ). Deglycosylation resulted in a 0.22-fold decrease in activity for r-Rha1- vitro and due to its slower turnover rate, r-Rha1- vivo showed a 0.73-fold decrease in activity. r-Rha1- vitro maintained the similar optimal temperature as r-Rha1, r-Rha1- vivo displayed a 10 °C lower optimal temperature. Compared to the decreased extent of r-Rha1- vitro in t 1/2 at 55 °C, 60 °C, and 65 °C and T m , chemical interferent deglycosylation in vivo showed a more profound impact on r-Rha1. Analyses based on circular dichroism, fluorescence spectroscopy, and differential scanning calorimetry revealed significant changes in the structure and thermodynamic stability of r-Rha1- vivo , accounting for its marked decline in activity and stability. The significant and unpredictable structure changes of r-Rha1- vivo proved the essential role of glycosylation for appropriate folding in P. pastoris .
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Yao Lu,Di Liu,Long Wang,Yongai Ma,Tai-Ping Fan,Huaxiang Deng,Yujie Cai
(−)-α-Bisabolol exhibits analgesic, anti-inflammatory, and skin-soothing properties and is widely applied in the cosmetic and pharmaceutical industries. The use of plant essential oil distillation or chemical synthesis to produce (−)-α-bisabolol is both inefficient and unsustainable. Currently, the microbial production of (−)-α-bisabolol mainly relies on Escherichia coli and Saccharomyces cerevisiae as chassis strains; however, high concentrations of (−)-α-bisabolol have certain toxicity to the strain. This study uses synthetic biology and metabolic engineering strategies to redesign a solvent-tolerant Serratia marcescens for the efficient production of (−)-α-bisabolol. By introducing the Haloarchaea-type mevalonate (MVA) pathway and the (−)-α-bisabolol biosynthesis pathway, we successfully constructed a strain capable of producing (−)-α-bisabolol. The coexpression of the chaperone protein DnaK/J significantly enhanced the soluble expression of the (−)-α-bisabolol synthase, resulting in a 10% increase in (−)-α-bisabolol titer. Furthermore, knockout of the PhoA gene, which reduced the formation of the byproduct farnesol (FOH), further increased the (−)-α-bisabolol titer to 3.21 g/L. In a 5 L bioreactor, S. marcescens achieved a final (−)-α-bisabolol titer of 30.2 g/L, representing the highest titer reported to date. This research provides guidance for the production of (−)-α-bisabolol in nonmodel microorganisms without the requirement for induction.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Nana Li,Ying Han,Wanqiu Huang,Liangqiang Chen,Mengdi Hao,Huabin Tu,Hao Shen,Shaoning Yu,Fan Yang
In natural world, sophisticated cascade reactions occur in compartmentalized organelles. However, simulating cascade processes in living systems remains problematic, and the drawbacks of enzymes, including dissatisfactory stability, reusability, and sensitivity in extreme microenvironments, have hampered further applications. Here, we report a confined multi-enzyme system in which Mn Fe metal-organic framework (MFM) is applied to encapsulate enzymes through shell-by-shell epitaxial-overgrowth in diverse compartments. MFM possessed excellent peroxidase-mimicking activity and biocompatibility, enabling it to serve not only as a reliable carrier for multiple enzymes, but also as a high-performance nanozyme in cascades. Compared with free enzymes, this system exhibited significantly improved bioactivity and environmental tolerance. On this basis, the confined multi-enzyme system was applied for selective α-amylase analysis in complicated fermentation specimens with a broad linear range (5–500 U·L −1 ) and low detection limit (2.14 U·L −1 ). This work sheds new light on the construction of efficient biocatalytic cascades to accelerate applications in food manufacturing.
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Food Bioscience
·
2024
·
IF 4.8
Qin Zhou,Yishi Wei,Yijing Liao,Xing Hu,Deming Gong,Guowen Zhang
Identification of new α -glucosidase ( α -Glu) inhibitors from natural sources is very important to the management of type 2 diabetes. In this work, liquiritigenin (LTG), a flavonoid compound derived from liquorice, was found to have excellent inhibition of α -Glu (IC 50 = 27.91 ± 3.48 μM) in a mixed manner. LTG reduced the endogenous fluorescence of α -Glu in a static mode by spontaneously forming stable complexes with the α -Glu-substrate through hydrophobic forces and hydrogen bonding. LTG decreased the α -helix and β -sheet contents of α -Glu while increasing the β -turn and random coil contents. Computer simulation showed that LTG interacted non-covalently with Gly161, Glu422, Glu429, and Asn235 residues of α -Glu, causing structural unfolding of the enzyme, which affected the substrate-enzyme interaction and ultimately reduced the release of the product. While acarbose, a competitive inhibitor of α -Glu, spontaneously hydrogen-bonded Tyr158, His280, Asp242, and Asp352 residues of the LTG− α -Glu complex. Moreover, a combination of LTG (6.00 μM) and acarbose (30.00 μM) had a remarkable synergistic inhibition on α -Glu, which may be because LTG made the structure of α -Glu loosening and reduced its catalytic activity through allosteric effect. And the occupation of the active cavity by acarbose may lead to a conformational change in the LTG− α -Glu complex, which effectively hindered the binding between the substrate and α -Glu. These findings may offer a new theoretical foundation for the development of LTG as a glucose-lowering ingredient in functional foods.
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ACS Infectious Diseases
·
2024
·
IF 4
Shu Li,Zhaopeng Wang,Shibo Song,Yuanyuan Tang,Jingjing Zhou,Xiaojing Liu,Xingjiao Zhang,Min Chang,Kairong Wang,Yali Peng
Multidrug resistance against conventional antibiotics has dramatically increased the difficulty of treatment and accelerated the need for novel antibacterial agents. The peptide Tat (47–57) is derived from the transactivating transcriptional activator of human immunodeficiency virus 1, which is well-known as a cell-penetrating peptide in mammalian cells. However, it is also reported that the Tat peptide (47–57) has antifungal activity. In this study, a series of membrane-active hydrocarbon-stapled α-helical amphiphilic peptides were synthesized and evaluated as antibacterial agents against Gram-positive and Gram-negative bacteria, including multidrug-resistant strains. The impact of hydrocarbon staple, the position of aromatic amino acid residue in the hydrophobic face, the various types of aromatic amino acids, and the hydrophobicity on bioactivity were also investigated and discussed in this study. Among those synthesized peptides, analogues P3 and P10 bearing a l-2-naphthylalanine (Φ) residue at the first position and a Tyr residue at the eighth position demonstrated the highest antimicrobial activity and negligible hemolytic toxicity. Notably, P3 and P10 showed obviously enhanced antimicrobial activity against multidrug-resistant bacteria, low drug resistance, high cell selectivity, extended half-life in plasma, and excellent performance against biofilm. The antibacterial mechanisms of P3 and P10 were also preliminarily investigated in this effort. In conclusion, P3 and P10 are promising antimicrobial alternatives for the treatment of the antimicrobial-resistance crisis.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2024
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IF 3.1
Xinlin Chen,Ying Wu,Sifang Wu,Yucheng Gu,Jianguang Luo,Lingyi Kong
Identifying medicinally relevant compounds from natural resources generally involves the tedious work of screening plants for the desired activity before capturing the bioactive molecules from them. In this work, we created a paper-based ligand fishing platform to vastly simplify the discovery process. This paper-based method exploits the enzymatic cascade reaction between α -glucosidase (GAA), glucose oxidase (GOx), and horseradish peroxidase (HRP), to simultaneously screen the plants and capture the GAA inhibitors from them. The designed test strip could capture ligands in tandem with screening the plants, and it features a very simply operation based on direct visual assessment. Multiple acylated flavonol glycosides from the leaves of Quercus variabilis Blume were newly found to possess GAA inhibitory activities, and they may be potential leads for new antidiabetic medications. Our study demonstrates the prospect of the newly discovered GAA ligands as potential bioactive ingredients as well as the utility of the paper-based ligand fishing method.
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JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
·
2018
·Vol. 133
·期 2
·859-867
·
IF 2.209
He Yu,Zhang Nan,Yuan Yanping,Cao Xiaoling,Sun Liangliang,Song Yanlin
In this study, iron oxide nanoparticles (α-Fe 2 O 3 ) have been firstly used as a nucleating agent, which simultaneously reduces the supercooling degree of sodium acetate trihydrate (SAT) and improves its thermal conductivity. A series of SAT composite phase change materials (PCMs) for potential latent heat thermal energy storage applications were prepared by a ball milling method using carboxymethyl cellulose as a thickening agent and sodium dodecyl sulfonate as a dispersant. In order to investigate the effect of the mass ratio of α-Fe 2 O 3 nanoparticles on the supercooling degree of SAT, various α-Fe 2 O 3 contents (0.2, 0.4, 0.6, 0.8, and 1.0 mass%) were added into the SAT matrix. It was found that the supercooling degree of the SAT composite PCM was reduced to 0 °C at a α-Fe 2 O 3 content of 0.8 mass%. Furthermore, no chemical reaction between SAT and α-Fe 2 O 3 occurred, and the presence of α-Fe 2 O 3 had no effect on the energy storage capability of SAT. The thermal conductivity of the SAT composite PCM was improved by 22.5% due to the addition of 0.8 mass% α-Fe 2 O 3 . After 60 melting–freezing cycles, the composite PCMs retained excellent stability with a small reduction in the phase change temperature (0.33 °C) and low latent heat loss rate (0.796%).
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Andi Suo,Gongjian Fan,Caie Wu,Tingting Li,Xiaojing Li,Dandan Zhou,Kaiping Cong,Xin Cheng,Wenjuan Sun
Dielectric barrier discharge (DBD) was a commonly used non-thermal plasma (CP) technology. This paper aimed to enhance the biological activity of apricot polysaccharides (AP) by using dielectric barrier discharge (DBD-CP) assisted H 2 O 2 -VC Fenton reaction for degradation. The degradation conditions were optimized through response surface methodology . The molecular weight (Mw) of degraded apricot polysaccharides (DAP) was 19.71 kDa, which was 7.25 % of AP. The inhibition rate of DAP (2 mg/mL) was 82.8 ± 3.27 %, which was 106.87 % higher than that of AP. DBD-CP/H 2 O 2 -VC degradation changed the monosaccharide composition of AP and improved the linearity of polysaccharide chains. In addition, a novel apricot polysaccharide DAP-2 with a Mw of only 6.60 kDa was isolated from DAP. The repeating units of the main chain of DAP-2 were →4)-α-D-Gal p A-(1 →, the branch chain was mainly composed of α-D-Gal p A-(1 → 2)-α-L-Rha p -(1→ connected to O-3 position →3,4)-α-D-Gal p A-(1→. The complex structure formed by the combination of DAP-2 and α-glucosidase was stable. DAP-2 had a higher α-glucosidase binding ability than the acarbose . These results suggested that DAP-2 had the potential to be developed as a potential hypoglycemic functional food and drug.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Zhao-Rong Li,Rui-Bo Jia,Yurong Mo,Haozheng Wang,Donghui Luo,Chunxia Zhou,Mouming Zhao
The current study compared the radical scavenging and α-glucosidase inhibition potentials of ellagic acid (EA) and its metabolites, urolithins (Uros), and further explored the structure–activity relationship. The outcomes indicated that urolithin M5 (Uro-M5), EA, and urolithin M6 (Uro-M6) exhibited superior 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity; EA and urolithin D (Uro-D) expressed better ABTS scavenging ability, and EA and Uro-M5 showed preferable α-glucosidase inhibition activity. The results of CD spectra and fluorescence spectral analysis explained the interaction between Uros and α-glucosidase. Correlation analysis indicated that hydroxyl groups were crucial for the antioxidative effect, while C-8 OH contributed greatly to the α-glucosidase inhibition activity. Quantum mechanical analysis showed that both EA and Uros exhibited strong electrophilic properties. These comparative results showed a biological discrepancy between Uros and provided essential information for exploring the bioactive application of EA as a functional ingredient or dietary supplement.
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