ANALYTICAL CHEMISTRY
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2020
·
IF 6.785
Guohua Qi,Dan Sun,Yu Tian,Chen Xu,Ying Zhang,Dandan Wang,Kongshuo Ma,Shuping Xu,Yongdong Jin
Caspase-3 is considered as one of the key proteases that can spontaneously regulate the life activities of cells, and its activation (usually is a slow process) will execute the apoptosis process of cells. Rapid activation of caspase-3 on demand in living-cells is therefore highly desired toward precise cancer therapy but it is still a key challenge. Herein, we applied electrostimulus (ES) to achieve fast activation of caspase-3 and trigger cell apoptosis, and developed a smart magnetic-plasmonic assembly nanoprobes (A-nanoprobes) to real-time trace cellular caspase-3 activation at the single cell level. The designer core–satellite A-nanoprobe, working specific to the activated caspase-3 via a disassembly tactic, provides strong “hot spots” to improve the sensitivity and therefore enables SERS sensing of cellular caspase-3 upon activated by ES. Single-cell analysis revealed that the ES can rapidly activate the apoptosis pathway of caspase-3 on demand to make the DNA fragmentation and ultimately induce the cell apoptosis. Such method and nanoplatform were further used to monitor ES-triggered caspase-3 activation in cell apoptosis process of different cell types, revealing that more caspase-3 will be activated for cancerous cells than normal cells during the ES to induce cells apoptosis. This strategy and platform are promising for detecting cellular caspase-3 and other enzymes in the process of cancer diagnosis and treatments.
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高影响因子
Chemical Engineering and Processing-Process Intensification
·
2020
·
IF 3.731
Guangying Chen,Guangjie Chen,Fan Cao,Rui Zhang,Hongxia Gao,Zhiwu Liang
3-Diethylaminopropylamine (DEAPA), a diamine with high reaction rate and large absorption capacity, was investigated in this work to evaluate its CO 2 absorption mass transfer performance in a hollow fiber membrane contactor in terms of the CO 2 absorption flux (J CO2 ). A series of experiments were conducted at various operation conditions and the effects of several influential parameters on J CO2 were examined and discussed in detail, including the liquid flow rate, the inlet gas flow rate, amine concentration, feed liquid temperature, CO 2 partial pressure, lean CO 2 loading, and membrane contactor height. Experimental results indicated that lean CO 2 loading and membrane contactor height have negative effect on the total mass transfer performance since the value of J CO2 decreases with the increase of these two factors, while the other five parameters exhibit positive effect and their increase lead to the increase of J CO2 values. In addition, a numerical correlation was established to predict the overall gas phase mass transfer coefficient and the CO 2 absorption flux, and the average absolute deviation (AAD) is 6.84%.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2020
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IF 4.939
Fan Yang,Ting Dong,Xixin Zhang,Jingjing Liu,Wentao Tian,Yifei Zhang
Semiconductor ionic electrolytes have obtained much attention because of good ionic conductivity and electrochemical performance. Novel semiconductor ionic NSDC (Ce 0.8 Sm 0.2 O 2-δ -Na 2 CO 3 )-LCCN (LiCo 0·225 Cu 0·075 Ni 0·7 O 3-δ ) composite materials have been adopted as electrolyte membrane for the first time, in which symmetrical cell composed of NSDC-LCCN membrane is constructed with Ni 0·8 Co 0·15 Al 0·05 LiO 2 (NCAl)-pasted Ni foam electrodes. An open circuit voltage (OCV) above 1 V and improved power density are obtained in the NSDC-LCCN cells, which confirms the functionality of the proposed semiconductor ionic materials. Meanwhile, X-ray diffractometer (XRD) and Scanning electron microscope (SEM) analyses identify the phase purity and homogenous nanocomposite morphology of all the NSDC-LCCN materials samples with various mass ratios. The performance illustrated by much more steady instead of transient state evaluation reveals that 3NSDC-LCCN composite electrolyte is most optimum, and the corresponding cell exhibits a considerable maximum power density of 598 mW cm −2 at 550 °C, over five times of that of pure NSDC electrolyte cells . Short-term duration test of 3NSDC-LCCN cell at 550 °C shows that the cell could steadily operate up to ~9 h without obvious degradation at a remarkable current density of 469 mA cm −2 , which indicates that NSDC-LCCN composite electrolyte is a promising material for low temperature solid oxide fuel cells .
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DALTON TRANSACTIONS
·
2020
·Vol. 49
·期 31
·10874-10879
·
IF 4.174
Runqing Liu,Hongping Wu,Hongwei Yu,Zhanggui Hu,Jiyang Wang,Yicheng Wu
Three rare-earth orthoborates Ba2MgY2(BO3)4, Ba2CdY2(BO3)4, and Ba2CdSc(BO3)3 were synthesized via a high-temperature solution method and their structures were determined by single crystal X-ray diffraction for the first time. Ba2MgY2(BO3)4 and Ba2CdY2(BO3)4 are isostructural and both crystallize in the monoclinic crystal system, P21/c, while Ba2CdSc(BO3)3 crystallizes in the triclinic crystal system, P. The structures of Ba2MgY2(BO3)4 and Ba2CdY2(BO3)4 can be described as the three-dimensional (3D) frameworks composed of MO5 (M = Mg, Cd), YOn (n = 6, 7) polyhedra with isolated BO3 and Ba2+ cations residing in the space of the frameworks. The structure of Ba2CdSc(BO3)3 is composed of 2D [CdSc(BO3)3]∞ layers separated by Ba2+ cations. In addition, structural comparisons, thermal behaviors, UV-vis-NIR diffuse reflectance spectra, and infrared spectroscopy results are also reported.
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高影响因子
BULLETIN OF MATERIALS SCIENCE
·
2020
·Vol. 43
·期 1
·1-6
·
IF 1.392
Li Lili,Yang Lin,Fu Bowen,Li Zhiqiang
Herein, we demonstrate a hot-injection method towards the fabrication of \(\hbox {Sb}_{2}\hbox {S}_{3}\) . The evolution from amorphous \(\hbox {Sb}_{2}\hbox {S}_{3}\) nanoparticles to a sheaf of \(\hbox {Sb}_{2}\hbox {S}_{3}\) nanorods cross-linked together occurring with the increase of reaction temperature and time is studied. The structural, compositional and morphological features of \(\hbox {Sb}_{2}\hbox {S}_{3}\) products indicate the formation of crystalline \(\hbox {Sb}_{2}\hbox {S}_{3}\) with orthorhombic phase and high purity. As the reaction proceeds, it can be observed that the individual nanorod grows along the elongated direction ( c -axis), and it is noteworthy that its round cross-section could develop into the rectangular cross-section subsequently. In this study we also propose a possible formation mechanism for the growth process of \(\hbox {Sb}_{2}\hbox {S}_{3}\) , and it reveals that the present hot-injection could provide an ideal growth environment for synthesizing the nanostructured materials by optimizing the experimental parameters.
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World Journal of Stem Cells
·
2020
·
IF 3.231
Jun-Hao Lei,Wen Yan,Chun-Hua Luo,Yu-Ming Guo,Yang-Yang Zhang,Xing-Huan Wang,Xin-Jun Su
BACKGROUND With continuous advancement of industrial society, environmental pollution has become more and more serious. There has been an increase in infertility caused by environmental factors. Nonylphenol (NP) is a stable degradation product widely used in daily life and production and has been proven to affect male fertility. However, the underlying mechanisms therein are unclear. Thus, it is necessary to study the effect and mechanism of NP on spermatogonial stem cells (SSCs). AIM To investigate the cytotoxic effect of NP on SSCs via the phosphatidylinositol-3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/AKT/mTOR) pathway. METHODS SSCs were treated with NP at 0, 10, 20 or 30 µmol. MTT assay was performed to evaluate the effect of NP on the proliferation of SSCs. Flow cytometry was conducted to measure SSC apoptosis. The expression of Bad, Bcl-2, cytochrome-c, pro-Caspase 9, SOX-2, OCT-4, Nanog, Nanos3, Stra8, Scp3, GFRα1, CD90, VASA, Nanos2, KIT, PLZF and PI3K/AKT/mTOR-related proteins was observed by western blot, and the mRNA expression of SOX-2, OCT-4 and Nanog was detected by quantitative reverse transcription polymerase chain reaction. RESULTS Compared with untreated cells (0 μmol NP), SSCs treated with NP at all concentrations showed a decrease in cell proliferation and expression of Bcl-2, Nanog, OCT-4, SOX-2, Nanos3, Stra8, Scp3, GFRα1, CD90, VASA, Nanos2, KIT, and PLZF ( P < 0.05), whereas the expression of Bad, cytochrome-c, and pro-Caspase 9 increased significantly ( P < 0.05). We further examined the PI3K/AKT/mTOR pathway and found that the phosphorylation of PI3K, AKT, mTORC1, and S6K was significantly decreased by NP at all concentrations compared to that in untreated SSCs ( P < 0.05). NP exerted the greatest effect at 30 μmol among all NP concentrations. CONCLUSION NP attenuated the proliferation, differentiation and stemness maintenance of SSCs while promoting apoptosis and oxidative stress. The associated mechanism may be related to the PI3K/AKT/mTOR pathway.
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MACROMOLECULES
·
2020
·
IF 5.918
Rui Qi,Yulin Zhu,Liang Han,Meijing Wang,Feng He
Fabrication of two-dimensional (2D) nanomaterials by conjugated polymers attracts increasing interest on account of their potential applications in optoelectronic devices. Precise control over the shape and size of the 2D nanostructures, however, is still challenging. We have developed a series of 2D rectangular micelles by hierarchical self-assembly of poly(3-hexylthiophene) (P3HT)-b-poly(ethylene glycol) (PEG) in i-PrOH. The aspect ratios and the sizes of the 2D rectangular micelles are controlled by the ratios of P3HT and PEG blocks, and the sizes of these 2D micelles are strongly concentration-dependent. Large and complex assembled architectures can be obtained in highly concentrated solutions. It is found that the block copolymers first assemble into fibers and then reorganize into 2D rectangular micelles with π–π interactions playing a key role in the formation of the 2D micelles. This offers a new method to prepare 2D nanostructures with controllable shapes and sizes for organic electronic devices.
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OPTICAL MATERIALS
·
2019
·
IF 2.687
Jidong Deng,Jiao Xun,Rongxing He
All-inorganic cesium lead halide perovskite nanocrystals (NCs) are promising photoelectric materials for their high photoluminescence quantum yield (PLQY), narrow emission width and tunable optical band gap. Traditionally, the hot injection (HI) method has been utilized to prepare perovskite NCs. However, the HI method requires high temperature, protection under inert gas and other harsh synthetic conditions, which severely hinders large-scale production of these NCs. Herein, we directly synthesized CsPbBr 3 perovskite NCs by a modified LARP (ligand-assisted re-precipitation) approach, and then conducted a specific anion exchange reaction with inexpensive, readily available, non-toxic, and non-polluting KX (X = Cl, I) salts aqueous solution to obtain various compositions of CsPbX 3 perovskite NCs at room temperature (RT). The obtained RT-NCs have high PLQY and outstanding stability, and their photoluminescence can be precisely regulated across the whole visible region (419–670 nm). The use of inorganic salts for the ion exchange provides an efficient method for the design of various halogenated perovskite NCs and their large-scale synthesis at RT.
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MOLECULES
·
2023
·Vol. 28
·期 23
·7823
·
IF 4.6
Ke Deng,Yafei Wang,Lei Wang,Xianli Fan,Zhenyu Wu,Xue Wen,Wen Xie,Hong Wang,Zheng Zhou,Pengfei Chen,Xianggui Chen
Phase transition behaviors of poly(N-isopropylacrylamide) nanogels with different compositions induced by (−)-epigallocatechin-3-gallate (EGCG) and ethyl gallate (EG) has been investigated systematically. Monodisperse poly(N-isopropylacrylamide-co-N-hydroxymethyl acrylamide) (P(NIPAM-co-NMAM)) and poly(N-isopropylacrylamide-co-2-hydroxyethyl methacrylate) (P(NIPAM-co-HEMA)) nanogels with different feeding monomer ratios were prepared by emulsion polymerization. P(NIPAM-co-NMAM) nanogels exhibit rapid isothermal phase transition behavior in EGCG solutions with low concentration (10−3mol/L) in less than 10 minutes. The thermosensitive phase transition behaviors of nanogels are affected not only by the copolymerized monomers but also by the concentrations of EGCG and EG in aqueous solutions. Nanogels remain in a shrunken state and do not exhibit thermosensitive phase transition behaviors in EGCG solutions (≥5 mmol/L), whereas they display thermo-responsive phase transition behaviors in EG solutions. The volume phase transition temperature (VPTT) shifts to lower temperatures with increasing EG concentration. The diameters of P(NIPAM-co-NMAM) nanogels decrease with increasing EG concentration at temperatures between 29 and 33 °C. In contrast, the diameters of P(NIPAM-co-HEMA) nanogels increase with increasing EGCG concentration at temperatures between 37 and 45 °C. The results demonstrate the potential of nanogels for simple detection of EG and EGCG concentrations in aqueous solutions over a wide temperature range, and EGCG can serve as a signal for the burst-release of drugs from the P(NIPAM-co-NMAM)-based carriers at physiological temperature.Keywords:thermo-responsive nanogel;poly(N-isopropylacrylamide-co-N-hydroxymethyl acrylamide);poly(N-isopropylacrylamide-co-2-hydroxyethyl methacrylate);(−)-epigallocatechin-3-gallate;phase transition
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高影响因子
ANALYST
·
2023
·
IF 4.2
Yueyuan Zhuang,Han Dong,Tianqing Liu,Yongmei Zhao,Yan Xu,Xiaojuan Zhao,Dan Sun
Caspase-3 is an important biomarker for the process of apoptosis, which is a key target for cancer treatment. Due to its low concentration in single cells and the structural similarity of caspase family proteins, it is exceedingly challenging to accurately determine the intracellular caspase-3 during apoptosis in situ. Herein, a biosensing strategy based on the target-induced SERS “hot spot” formation has been developed for the simultaneous highly sensitive and selective detection of intracellular caspase-3 level. The nanosensor is composed of gold nanoparticles modified with the probe molecule 4-mercaptophenylboronic acid (4-MPBA) and a peptide chain. The well-designed peptide chain contains two distinct functional domains, one with a sulfhydryl group for bonding to the gold nanoparticles and the other a fragment specifically recognized by caspase-3. When caspase-3 is present, the negatively charged segment (NH2–Asp–Asp–Asp–Glu–Val–Asp–OH) of the peptide chain is specifically hydrolyzed, leaving a positively charged fragment coated on the surface of the gold nanoparticles. At this time, the golden nanoparticles undergo significant coupling aggregation due to the electrostatic interaction, resulting in a large number of SERS “hot spot” formation. The SERS signal of the 4-MPBA located at the nano-gap is significantly boosted because of the local plasma enhancement effect. The highly sensitive determination of caspase-3 can be achieved according to the altered SERS signal intensity of 4-MPBA. The turn-on of the SERS signal-induced target contributes to the excellent selectivity and the formation of the SERS “hot spot” effect that further improves the sensitivity of caspase-3 detection. The advantages of this biosensing technique allow for the precise in situ monitoring of the dynamic changes in caspase-3 levels during apoptosis. In addition, the differences in caspase-3 levels during the apoptosis of various cell types were compared. Monitoring the caspase-3 levels can be used to track the cellular apoptosis process, evaluate the effect of drugs on cancer cells in real time, and provide guidance for the selection of the appropriate drug dosage.
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JOURNAL OF MEDICINAL CHEMISTRY
·
2021
·
IF 7.446
Hong Yao,Giuseppe Uras,Pengfei Zhang,Shengtao Xu,Ying Yin,Jie Liu,Shuai Qin,Xinuo Li,Stephanie Allen,Renren Bai,Qi Gong,Haiyan Zhang,Zheying Zhu,Jinyi Xu
Based on a multitarget strategy, a series of novel tacrine–pyrimidone hybrids were identified for the potential treatment of Alzheimer’s disease (AD). Biological evaluation results demonstrated that these hybrids exhibited significant inhibitory activities toward acetylcholinesterase (AChE) and glycogen synthase kinase 3 (GSK-3). The optimal compound 27g possessed excellent dual AChE/GSK-3 inhibition both in terms of potency and equilibrium (AChE: IC50 = 51.1 nM; GSK-3β: IC50 = 89.3 nM) and displayed significant amelioration on cognitive deficits in scopolamine-induced amnesia mice and efficient reduction against phosphorylation of tau protein on Ser-199 and Ser-396 sites in glyceraldehyde (GA)-stimulated differentiated SH-SY5Y cells. Furthermore, compound 27g exhibited eligible pharmacokinetic properties, good kinase selectivity, and moderate neuroprotection against GA-induced reduction in cell viability and neurite damage in SH-SY5Y-derived neurons. The multifunctional profiles of compound 27g suggest that it deserves further investigation as a promising lead for the prospective treatment of AD.
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CERAMICS INTERNATIONAL
·
2023
·
IF 5.2
Teng Su,Huanle Chen,Zhiyang Wei,Mingyuan Hao,Xiaodong Wang,Yufeng Liu,Chao Ma,Yang Miao,Feng Gao
In this study, based on the highly disordered multi-component system with high-entropy and traditional perovskite with high dielectric constant, a series of the Al 3+ -doped high-entropy perovskite (Zn 1/6 Ba 1/6 Ca 1/6 Sr 1/6 La 1/3 )Ti 1-x Al x O 3 (0 ≤ x ≤ 0.1) ceramics was designed and synthesized by using a solid state reaction method to explore the effects of different doping amounts on microwave dielectric characteristics together with the microstructures. The single-phase structure in space group Pm-3m was determined in the sintering temperature range of 1325 °C–1400 °C and both had high dielectric constant (from 74.46 to 93.44). With the increase of Al doping amount, the variation trend of dielectric constant was closely related to the density and ionic polarizability. The Q × f value of doped samples was higher than that of undoped samples, which was mainly due to the interaction of oxygen vacancy content and grain size. Due to the high-entropy effect resulting in [Ti-O6] octahedral torsion, the τ f value was greatly reduced compared with traditional perovskite, which exhibited outstanding microwave dielectric properties (ε r = 74.46, Q × f = 4108 GHz at 4.31 GHz, and τ f = +184 ppm/°C). The present work provided an effective approach to improve the properties of microwave dielectric ceramics .
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