Nanomaterials
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2024
·Vol. 14
·期 23
·1873
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IF 4.4
Elena V. Svirshchevskaya,Valentina V. Kostenko,Anna A. Boyko,Maxim Shevtsov,Roman V. Kholodenko,Maria V. Grechikhina,Iuliia A. Gracheva,Alexey Yu. Fedorov,Alexander M. Sapozhnikov
Anti-cancer targeted therapy is a promising approach. However, the identification of target molecules over-expressed in a wide range of tumors remains a significant challenge. The aim of this study was to analyze the expression of cell membrane-exposed heat shock protein 70 kDa (mHSP70) on different tumor cells and to develop a nanoscale delivery system based on a monoclonal antibody (mAb) that recognizes mHSP70 and uses chitosan core–shell nanoparticles (NPs). Several types of tumor cells (breast, pancreas, colon, prostate cancers, and some lymphomas) expressed mHSP70 as was determined by flow cytometry and confocal microscopy both in 2D and 3D cultures. Core NPs were formed by chitosan (C) conjugated to allocolchicinoid, which was used as a model drug (D). mAbs (A) targeting mHSP70 were complexed with succinylchitosan and used as NP shells forming final CAD-NPs. These NPs were characterized by size, charge, and functional activity. CAD-NPs were shown to have additional toxicity in comparison with CD-NPs in mHSP7-positive cells. Taken collectively, this study shows that mAb to mHSP70 can be used as a targeting vector in antitumor therapy.
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MACROMOLECULAR BIOSCIENCE
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2024
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IF 4.4
Dingkun Liu,Jinbing Chen,Linjuan Gao,Xing Chen,Liujun Lin,Xia Wei,Yuan Liu,Hui Cheng
Controlling infections while reducing the use of antibiotics is what doctors as well as researchers are looking for. As innovative smart materials, photothermal materials can achieve localized heating under light excitation for broad-spectrum bacterial inhibition. A polydopamine/chitosan/β-glycerophosphate temperature-sensitive hydrogel with excellent antibacterial ability is synthesized here. Initially, the hydrogel has good biocompatibility. In vitro experiments reveal its noncytotoxic property when cocultured with gingival fibroblasts and nonhemolytic capability. Concurrently, the in vivo biocompatibility is confirmed through liver and kidney blood markers and staining of key organs. Crucially, the hydrogel has excellent photothermal conversion performance, which can realize the photothermal conversion of hydrogel up to 3 mm thickness. When excited by near-infrared light, localized heating is attainable, resulting in clear inhibition impacts on both Staphylococcus aureus and Escherichia coli , with the inhibition rates of 91.22% and 96.69%, respectively. During studies on mice's infected wounds, it is observed that the hydrogel can decrease S. aureus ’ presence in the affected area when exposed to near-infrared light, and also lessen initial inflammation and apoptosis, hastening tissue healing. These findings provide valuable insights into the design of antibiotic-free novel biomaterials with good potential for clinical applications.
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JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY
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2025
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IF 1.9
Qifan Yu,Yifei Li,Yuxin Wang,Hanyu Zhang,Menghua Qi,Liangjie Song,Lei Chen,Zhishan Bai,Bingjie Wang
Multichannel integrated microfluidic technology retains the precise control characteristics of microfluidics. This technology is expected to replace traditional droplet preparation techniques for the batch production of adsorption materials with high performance and high monodispersity. In this study, porous chitosan microspheres (PCSM) were successfully synthesized using a self-developed modularized microfluidic system. As-prepared PCSMs were employed in adsorption separation of methyl orange (MO). The results proved that the average droplet size of PCSMs was 892 μm with the lowest coefficient of variation (CV), only 2.75%, while, the droplet flux was up to 210 mL·h−1. Batch static adsorption experiments showed that the saturated adsorption capacity of the adsorbent reached 215.26 ± 2.22 mg·g−1. Besides, the adsorption kinetics and equilibrium adsorption isotherm fitted pseudo-second-order kinetic model and Langmuir isotherm model well, respectively, indicating that the adsorption process is dominated by chemical reaction. Moreover, continuous adsorption experiment showed that under the conditions of initial solution concentration of 40 mg·L−1, bed diameter of 15 mm and filling height of 9.3 mm, the breakthrough time was 140 min, and the maximum adsorption capacity was up to 233.3 ± 4.30 mg·g−1. In addition, PCSMs exhibited stable regeneration performance and efficient selective adsorption for MO dyes in recycling (73.4% reabsorption productivity after 5 cycles). This study can provide theoretical support for the microfluidic batch synthesis and industrial application evaluation of high-performance adsorbents.
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FOOD CHEMISTRY
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2025
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IF 9.8
Zi-Qiang Luo,Tao Peng,Ya Hu,Bin-Yang Yang,Jing Wang,Tao Zou
The selection of suitable porous materials as the adsorbent of mycotoxin is of great significance for food safety. In this work, hierarchical porous materials with high specific surface area and suitable pore size distribution were prepared by one-step carbonization method using chitosan and KMnO 4 as carbon precursors and activators, respectively. The best adsorption performance was achieved using the hierarchical porous materials prepared at carbonization temperature of 600 °C. The adsorption capacity and Brunauer-Emmett-Teller specific surface area was 8.4 μg/mg and 1800.76 m 2 g −1 , respectively. The adsorption of patulin on hierarchical porous materials conforms to the Langmuir and pseudo-second-order model. Moreover, the hierarchical porous materials did not have a significant effect on the nutrients of the apple juice. The adsorbents prepared herein has been found to be a good solution for the adsorption of patulin, owing to their easy preparation, low cytotoxicity, high efficiency, and good reusability.
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FOOD CHEMISTRY
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2025
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IF 9.8
Jie Chen,Hui Zhang,Yuxin Lin,Jinyu Liu,Yisong Chen,Xiaoyan Zhang,Hetong Lin,Da-Peng Yang
Food preservation technologies that combine antimicrobial activity with controlled-release functionality are essential to mitigate postharvest losses. Herein, chitosan (CS)-based coatings incorporating S-nitroso- N -acetyl-DL-penicillamine (SNAP) as a nitric oxide (NO) donor were developed for sustained antimicrobial release and fruit preservation. The CS/SNAP coatings showed high transparency (79.5–79.9 %), strong UV-blocking (T 350 nm < 1 %), and rapid water solubility, with flexibility increasing alongside SNAP loading. The coatings released NO over 15 days, exhibiting potent antibacterial effects against E. coli (24.64 mm inhibition zone), S. aureus (28.86 mm), and antifungal activity against B. cinerea (20.20 mm). When applied to tomatoes, the CS/SNAP12 coating reduces weight loss, maintains fruit firmness and inhibits ethylene production, thereby extending shelf life to 15 days. Cytotoxicity and seed germination tests confirmed biocompatibility. This scalable, eco-friendly solution synergistically controls respiration, microbial growth, and moisture loss, offering a promising approach to perishable food preservation.
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CARBOHYDRATE POLYMERS
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2022
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IF 10.723
Zhuqing Wan,Qinyuan Dong,Xiaodong Guo,Xiaoqiang Bai,Xiao Zhang,Ping Zhang,Yunsong Liu,Longwei Lv,Yongsheng Zhou
Delayed inflammatory reaction and poor osteogenesis are the two main causes of failure for bone-defect healing. Accordingly, in the present study, a dual-responsive hydrogel composite was successfully fabricated in which near-infrared (NIR)-light-responsive polydopamine-coated magnesium‐calcium carbonate microspheres are incorporated into a thermo-responsive hydroxybutyl chitosan hydrogel to provide sequential delivery of the anti-inflammatory drug aspirin and osteogenic bone morphogenetic protein 2 (BMP-2). By initially releasing aspirin rapidly, the hydrogel composite efficiently ameliorates early-stage inflammatory reaction and promotes transition to the regenerative phase. Then, the hydrogel composite allows NIR-light-responsive release of BMP-2, which maximizes its osteoinductive effects. Using an SD rat calvaria-defect model, the sequential and controllable release achieved by the hydrogel is demonstrated to promote new-bone formation. Thus, the current study provides an efficient alternative strategy for developing multifunctional therapeutic biomaterials for bone tissue engineering.
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ACS Applied Bio Materials
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2019
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IF 0
Jiangying Zhu,Guohua Jiang,Gao Song,Tianqi Liu,Cong Cao,Yuhui Yang,Yajing Zhang,Wenjie Hong
Development of proper skin wound dressing is a vital step for wound repair, especially for those patients with serious skin injuries. Herein, zinc-doped bioactive glass (ZBG)/succinyl chitosan (SCS)/oxidized alginate (OAL) composite hydrogels (Gel-ZBG) have been developed as wound dressings to accelerate wound closure. Schiff-based linkages have been introduced into the composite hydrogels, which provide a humid microenvironment for the proliferation of cells on wound sites. The amino groups from SCS and Zn2+ released from ZBG exhibited excellent antibacterial properties to composite hydrogels, confirmed by the antibacterial tests in vitro. Si4+ and Ca2+ ions are essential factors that stimulate fibroblasts to secrete beneficial factors for angiogenesis and wound closure. The epidermal growth factor (EGF) was further embedded into the hydrogels to improve cell proliferation and tissue remodeling in the wound bed. Finally, the formation of granulation tissue, deposition of collagen and myofibril, the release of anti-inflammatory factors, and angiogenesis have been investigated to determine the healing mechanism of the composite hydrogels as the wound dressings.
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CHEMICAL ENGINEERING JOURNAL
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2018
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IF 6.735
Yixuan Chen,Jianqing Yu,Qinfei Ke,Youshui Gao,Changqing Zhang,Yaping Guo
Effective repair of bone defects mainly depends on the osteoinductivity and biocompatibility of implants. Herein, we for the first time reported the bioinspired fabrication of carbonated hydroxyapatite/chitosan (CHA/CS) nanohybrid scaffolds using CaCO 3 /CS porous scaffolds as sacrificial templates, followed by loading TWS119 drugs (CHA/CS/TWS119). After treatment with phosphate buffer solutions, the CaCO 3 /CS porous scaffolds were in situ converted into CHA/CS nanohybrid scaffolds via the dissolution-precipitation reaction. The as-formed CHA nanoplates with thicknesses of ∼20 nm and widths of 1.0–2.0 μm were perpendicular to the scaffold surfaces. The three-dimensional interconnected macropores supported the adhesion and spreading of bone mesenchymal stem cells (BMSCs), and the mesopores within the CHA nanoplates as drug delivery channels improved the drug loading-release properties. The controlled drug release of the CHA/CS/TWS119 nanohybrid scaffolds significantly enhanced the osteogenic differentiation of BMSCs by the activated GSK3β/β-catenin pathway. Moreover, rat cranial defect models revealed the enhancing effects of the CHA/CS/TWS119 scaffold on bone tissue regeneration. The micro-CT, haematoxylin and eosin (H&E) staining, Van Gieson (VG) staining, Masson staining and double fluorochrome labelling results demonstrated that more new bone tissues were formed in the CHA/CS/TWS119 nanohybrid scaffolds than the control group without loading TWS119. Therefore, the CHA/CS/TWS119 nanohybrid scaffold is a promising therapeutic material for bone tissue regeneration.
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JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
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2020
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IF 2.69
Chaochen Zhao,Yang Li,Gongze Peng,Xiongxin Lei,Guifeng Zhang,Yi Gao
A bioreactor filled with functional hepatocytes is a crucial portion of the bio-artificial liver device. However, it is a difficult task to maintain sufficient cell quantity and active hepatocellular function. In this work, we developed a promising scaffold for hepatocyte culture by coating porcine liver extracellular matrix (ECM) on chitosan (CTS) fabrics. Porcine Liver was decellularized using 1% Triton X-100. Solubilized liver ECM was immobilized on CTS fibers surface through cross linking of ECM and CTS with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-Hydroxysuccinimide (NHS). Then the scaffold was characterized by Fourier transformed infrared spectroscopy in attenuated total reflection mode (ATR–FTIR), X-photoelectron spectroscopy (XPS) and water contact angle measurement. The efficacy of modified scaffolds to maintain C3A hepatocytes adhesion, proliferation, bioactivity and functionality in vitro was detected. FTIR spectra and XPS demonstrated the presence of ECM coating on CTS fabric surface. Covalently attached coating significantly improved the binding efficiency between ECM and CTS fabrics, in comparison to the coating by physical absorption. Furthermore, C3A hepatocytes cultured on coated scaffolds showed enhanced cell bioactivity and liver-specific function, such as albumin secretion and urea synthesis, compared with those cultured on untreated scaffolds(p < 0.05). As a promising hepatocyte culture carrier, the ECM coated CTS fabrics could be applied in the biological artificial liver reactor.
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FOOD RESEARCH INTERNATIONAL
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2025
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IF 8
Huanyu Zheng,Ji Zhang,Zhouliang Sun,Mengqi Wang,Yufei Zhang,Mingming Zheng
Nature employs non-catalytic scaffold proteins to achieve precise enzyme positioning and enhanced catalysis. However, mimicking this approach with food-grade macromolecular materials remains a significant challenge. Herein, we introduce a pH-tunable gliadin-chitosan matrix designed to act as a bioinspired scaffold. It spontaneously self-assembles with lipase into stable ternary complex, gliadin-chitosan-lipase (GCL), through robust hydrogen bonding and hydrophobic interactions (∆G_binding = −331.7 kJ‧mol −1 ) immobilize lipase AYS in an optimally active conformation. Upon addition to an oil-water mixture at pH 7, GCL particles adsorb at the interface, creating a highly efficient Pickering interfacial biocatalysis (PIB) system. When applied to the synthesis of sterol esters via interesterification with flaxseed oil, the GCL-stabllized PIB system achieved an 81.4 % yield within 6 h, a relative conversion rate of almost three times that of free lipase (31.8 %), and maintained 73.4 % of its initial activity after five consecutive cycles. Its compatibility extended to six commercial oils. Molecular dynamics results indicated that lipase acts as a regulatory element, suppressing global fluctuations to generate stable structures with high catalytic activity, thereby preparing the interface for biocatalysis. This work establishes a sustainable, scalable platform for functional lipid production and provides a blueprint for next-generation biomacromolecular enzyme carriers.
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Gels
·
2025
·Vol. 12
·期 1
·20
·
IF 5.3
Jun An,Liyou Zheng,Shuzhen Xuan,Xinyi He,Tao Yang
As a step towards the substitution of saturated fats with camellia oil in foods, a camellia oil-based oleogel was prepared using a walnut protein isolate–chitosan (WPI-CS) composite via an emulsion template method. The preparation process, structural characteristics, and stability of the oleogel were systematically analyzed. Our findings showed that varying the ratio of WPI-CS to camellia oil (CO) effectively regulated the emulsion particle size, zeta potential, and viscosity, thereby subsequently influencing the oil-holding capacity (OHC), rheological properties, and thermal stability of the oleogel. When the WPI-CS:CO ratio was 13:7, the oleogel exhibited superior performance, including relatively high OHC, improved rheological properties, and excellent thermal stability. In addition, the OHC of the oleogel varied significantly with temperature, and high oxidative stability was observed at WPI-CS ratios such as 13:7–10:10. Application tests in cake formulations demonstrated that the oleogel has potential as a partial butter replacement. This study provides a theoretical basis for the construction of WPI-based oleogels and offers new insights for the development of healthy fat substitutes.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Yu Liu,Jie Chen,Chunyan Hu,Baojiang Liu
Adhesive conductive hydrogels have a broad application prospect in the field of flexible sensors, but existing adhesive hydrogels often struggle to balance adhesion performance and mechanical strength, and their adhesion stability is poor, which limits their long-term applications. In this study, a dual-network-structured hydrogel with durability, conductivity, and adhesion was prepared using a photoinitiated polymerization process, and its potential application in flexible sensors was explored. The hydrogel is based on thiosemicarbazone-modified chitosan (CS-SCM) as the backbone, introducing a reducible group into the system, acrylic acid (AA), tannic acid (TA), and Fe3+ are incorporated, endowing the hydrogel with excellent electrical conductivity and adhesion durability through multiple hydrogen bonds, electrostatic interactions, and metal-phenol complexation effects. The experimental results show that the hydrogel is able to maintain stable and long-lasting adhesion on the surface of various substrates, and its adhesion to plank remains above 90 kPa after 5 days of storage and more than 20 kPa after 13 days, which is significantly better than traditional hydrogels. Moreover, the durable adhesion of this hydrogel is highly valuable for research and applications in flexible sensors, smart wearables, and bioelectronic devices.
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