Advanced Healthcare Materials
·
2026
·
IF 11
Peng-Wei Chen,Yu-Meng Zhang,Lin-Song Li,Bang-Bang Liu,Han-Lu Sun,Liping Chen,Xiaojing Yang,Mei-Xia Zhao
The combination of phototherapy with oxaliplatin (OXA) represents a potent strategy for triggering immunogenic cell death (ICD) and elicit robust antitumor immunity. In this study, we construct IROTD nanoparticles (IROTD NPs) through self-assembly of a norcantharidin (NCTD)-OXA-conjugated polymer (P2) and the near-infrared (NIR) photosensitizer DTIR780. Under 808 nm laser irradiation, IROTD NPs generate heat and reactive oxygen species (ROS) while release OXA and NCTD, result in severe DNA damage and potent induction of ICD within tumor cells. Concurrently, NCTD inhibits DNA repair mechanisms and down-regulates PD-L1expression, thereby increase the infiltration of cytotoxic T-cell and amplify systemic antitumor immunity. Leverage the enhanced permeability and retention (EPR) effect, IROTD NPs efficiently accumulate at tumor sites, result in remarkable inhibition of tumor growth and recruitment of CD4 + and CD8 + T lymphocytes, which collectively contribute to the activation of a systemic antitumor immunity. In a bilateral tumor model, IROTD NPs effectively inhibit both primary and distant tumor growth. Overall, this work presents a rationally designed combinatorial nanoplatform that effectively ignites systemic immune response, thus establish a prospective chemo-immunotherapeutic stratagem for cancer treatment.
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TALANTA
·
2026
·
IF 6.7
Hengxin Yu,Guohai Lin,Jing Jiang,Miaomiao Cai,Yue Qiao,Huo Xu
Lead ions (Pb 2+ ), as a persistent and bioaccumulative environmental toxin, pose a significant threat to public health, demanding ultrasensitive and field-deployable detection methods. Conventional sensors often struggle with a trade-off between sensitivity and stability, especially when employing hard nanomaterial enhancers like gold nanoparticles. However, the practical utility of these inorganic nanoprobes is often compromised by their susceptibility to surface passivation (biofouling) and aggregation in salt-rich or protein-rich environments. Herein, we introduce a novel concept of a soft-matter DNA nanoreactor for engineering a high-performance colorimetric biosensor. This nanoreactor is self-assembled from a single palindromic DNA strand into a DNA nanosphere (DS), creating a spatially confined microenvironment. By anchoring a Pb 2+ -specific DNAzyme system onto this platform, we developed the DS-S-Dz probe. The nanoreactor architecture dramatically enhances the catalytic efficiency of the DNAzyme through a profound spatial confinement effect, which accelerates the Pb 2+ -triggered cleavage of a substrate strand and subsequent release of a G-rich sequence. This liberated sequence then folds into a G-quadruplex/hemin DNAzyme, catalyzing a robust colorimetric reaction. This strategy effectively suppresses background noise while amplifying the target signal, achieving a remarkable limit of detection of 0.96 nM, a 50-fold improvement over the non-amplified system (48 nM) and well below the EPA safety limit (72 nM). The sensor demonstrated exceptional selectivity against a panel of interfering metal ions and proved its robustness and accuracy in complex real-world samples like tap water and milk. This work presents a paradigm shift from simple nanoparticle enhancement to the rational design of programmable DNA nanoreactors, offering a powerful and elegant platform for sensitive and reliable environmental monitoring.
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BIOSENSORS & BIOELECTRONICS
·
2026
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IF 10.5
Xuewen Peng,Sufen Yang,Zhengzhang Huang,Niu Feng,Hetong Zhang,Yiqi Li,Yating Han,Caiyang Zhang,Jimei Ma,Yiping Chen
Pathogens have long presented a significant threat to human lives and environmental security, and the rapid detection of infectious pathogens is therefore vital for mitigating such risks. However, the practical application of biosensors is often hampered by complex sample matrices and limited sensitivity. To address these challenges, we established magnetic nanoparticle (MNP) functionalized with phenylboronic acid (PBA) (MNP-PBA) that efficiently and non-destructively captures Staphylococcus aureus ( S. aureus ) from complex samples within 20 min, achieving 92.21% capture rate at 10 8 CFU/mL. In addition, an aggregation-induced emission luminogen, TTQ-PF 6 , was introduced as a detection probe. TTQ-PF 6 exhibits a large Stokes shift (265 nm), intense red fluorescence, and exceptional anti-photobleaching ability—representing a 5.67-fold and 9.40-fold enhancement compared to Rhodamine X, respectively. Moreover, TTQ-PF 6 allows multiple fluorophores to be conjugated to a single DNA strand without the need for chemical reagents. The combination of MNP-PBA and red-emitting TTQ-PF 6 provides strong resistance to matrix interference in bacterial detection, realizing a broad detection linear range for S. aureus from 10 2 to 10 7 CFU/mL, with a limit of detection as low as 13.74 CFU/mL. Overall, this work provides a robust and efficient platform for pathogen detection in complex environments.
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JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2012
·Vol. 16
·期 8
·2837-2843
·
IF 2.131
Meng Xiaomeng,Yin Huanshun,Xu Minrong,Ai Shiyun,Zhu Jianying
Nonylphenol (NP) was determined using electrochemical method based on ionic liquid-functionalized graphene nanosheet modified electrode. The fabricated electrode was characterized by electrochemical impedance spectroscopy. The different influence parameters, such as pH value, scan rate, accumulation potential, and time, were investigated. Under the optimum conditions, the linear relationship between peak current value and concentration of NP was obtained with differential pulse voltammetry in two ranges from 0.5 to 30 μM and 30 to 200 μM with the detection limit of 0.058 μM ( S/N = 3). Moreover, this modified electrode was applied to NP determination in water and soil samples with the recoveries from 94.8 to 104 %, which showed the method could be applied to determine NP in environment. In addition, to explore the damage to DNA, the interaction between NP and DNA was investigated with the association constant ( β ) of 0.4867 and the Hill coefficient ( m ) of 3.75 × 10 5 M −1 , respectively.
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NEW JOURNAL OF CHEMISTRY
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2026
·
IF 2.6
Shilong Yang,Ying Tang,Mengyi Zhou,Wen Lu,Huayu Xue,Li Xu
Amentoflavone (Ame) is a kind of precious biflavonoids with various bioactivities. To further develop and use Ame, Ame was extracted and purified from ginkgo biloba leaves, and Ame complexes with metal ions (Mn 2+ /Fe 3+ /Co 2+ /Ni 2+ /Cu 2+ /Zn 2+ ) were first synthesized. The structures of Ame complexes were characterized by UV/Vis, FT-IR, HRMS and DFT calculation. The antioxidant capacity was evaluated by pyrogallol autoxidation and ABTS methods. DNA binding affinity were explored via fluorescence method and molecular docking. The structural analysis indicated the stoichiometric ratios of Ame and Mn 2+ /Co 2+ /Ni 2+ /Cu 2+ /Zn 2+ were 1:1, the chelation site was between 5''-hydroxyl and 4''-carbonyl. While the stoichiometric ratio of Ame and Fe 3+ was 2:2, and the chelation site was between 5''-hydroxyl and 4''-carbonyl, 5-hydroxyl and 4-carbonyl. The investigation of pyrogallol autoxidation and ABTS methods demonstrated Ame complexes had superior scavenging activities on O 2 -• and ABTS + • free radicals. What's more, scavenging free radical activity of some Ame complexes was stronger or similar comparing with VC. The results of fluorescence method indicated Ame complexes had stronger DNA binding affinity with intercalation mode. Molecular docking study confirmed Ame and its complexes interacted with DNA via Van der Waals, conventional hydrogen bond and π-π stacked effect. These results demonstrated bioactivities of Ame could be enhanced via forming complexes with metal ions and provided theoretical knowledge for biflavonoids acting as potential drug, dietary supplement and cosmetics.
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CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2026
·
IF 1.4
Ruiting Tian,Min Shi,Tao Fang,Uchkun Ishimov,Awais Ihsan,Jun Li,Syed Aftab Naqvi,Xianju Huang,Han Cheng
This study presents the development of an ultrasensitive electrochemical sensor based on a ruthenium nanoparticle-modified carbon fiber microelectrode (RuNPs/CFME) for the detection of aesculetin and investigation of its interaction with DNA. The RuNPs/CFME exhibited significantly enhanced electrocatalytic activity, attributed to the large specific surface area and excellent conductivity of RuNPs, resulting in 54.6% decrease in charge transfer resistance compared to the bare electrode. The sensor demonstrated a linear response to aesculetin in the concentration range of 0.01-1 μM, with a detection limit (LOD) of 1.62 nM and a quantification limit (LOQ) of 5.44 nM. The electrode process was adsorption-controlled and involved a two-electron transfer. The modified sensor displayed high selectivity against common interferents, excellent reproducibility (RSD < 2%), and stability (>95% signal retention after 10 days). Furthermore, the platform was applied to study the interaction between aesculetin and calf thymus dsDNA, revealing a binding mechanism dominated by electrostatic interactions, as evidenced by a negative shift in oxidation potential and current attenuation. The sensor was successfully employed for aesculetin detection in human serum samples, achieving recoveries of 95.7-102.6%, demonstrating its potential for pharmacological applications and drug-DNA interaction studies.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Runzi Zhang,Xiaoyu Yang,Shunbi Xie,Bo Wu,Yao Liu,Yi He
Herein, target-induced catalytic hairpin assembly (CHA) integrate with a pH-responsive DNA triple helix structure is first used to construct a recyclable layered covalent organic framework ( L -COF) @Au as a SERS substrate for the ultrasensitive detection of ofloxacin (OFL). Firstly, a large specific surface area and abundant -NH 2 groups of the L -COF serves as an ideal carrier for Au NPs, enabling the generation of a strong and uniform electromagnetic field. Meanwhile, target-triggered catalytic hairpin assembly (CHA) is utilized to activate an efficient DNA walker system, thereby achieving significant amplification of the SERS signal. Notably, pH-responsive folding and unfolding of the DNA triplex can enables capture and release of the SERS tag (Au@4-NTP@Ag) to achieve regeneration of the SERS substrate, thus allowing multiple reuse cycles. Furthermore, a synergistic plasmonic enhancement effect was realized between the COF@Au substrate and the SERS tag, resulting in a broad dynamic detection range from 1.0 × 10 −13 mol/L to 1.0 × 10 −6 mol/L and a low limit of detection (LOD) of 5.79 × 10 −14 mol/L. The biosensor demonstrates accurate quantification in real food samples, confirming its practical applicability. In addition, the methodology simplifies experimental procedures and reduces operational costs. Overall, this study presents a highly selective and responsive sensing approach for constructing renewable SERS-based biosensors, providing a robust and prospective platform for the monitoring of antibiotics in food safety applications.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2026
·
IF 4.6
Yaoxuan Wang,Haiyan Wang,Xianchuan Dong,Jing Sui,Feifei Zhang,Zonghua Wang
Here presents a sensing strategy for the detection of miRNA-155 based on a multi-amplification strategy, which involves nucleic acid recognition-induced multiple DNA release and Exonuclease III (Exo III)-assisted target recycling. Initially, cDNA-magnetic bead bioconjugates were employed to capture the target miRNA, triggering the release of two methylene blue (MB)-modified messenger DNAs (mDNAs). This step effectively doubles the amount of mDNAs-MB released, contributing to initial signal amplification. Subsequently, the target miRNA is recycled via Exo III-mediated cleavage, leading to the release of additional mDNAs-MB and further enhancing the signal. Finally, each capture probe (P1) is designed to hybridize with two mDNAs-MB, enabling dual signal recognition. As a result, the square wave voltammetry (SWV) measurements detected a significantly amplified MB peak current. The overall detection strategy integrates three synergistic amplification approaches: the release of multiple mDNAs, Exo III-assisted target miRNA recycling, and dual hybridization of mDNAs to P1. The miRNA-155, a recognized biomarker for breast cancer diagnosis, was used as the target to evaluate the biosensor's performance. The proposed electrochemical biosensor demonstrated a broad detection range from 0.1 pM to 1 mM and achieved a low detection limit of 0.026 pM, confirming its high sensitivity and potential applicability in cancer diagnostics.
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Acta Biomaterialia
·
2026
·
IF 10.4
Yu Liu,Ziwen Xiao,Yu Zhang,Qian Chen,Siyu Luo,Yaoqing Wang,Zhaohua Miao,Zhenchao Tao,Deyan Gong
Acute liver failure (ALF) can result from the progressive development of acute liver injury triggered by acetaminophen (APAP) overdose. The core mechanisms involve an imbalance in the antioxidant system and upregulation of the inflammatory response, which collectively induce ferroptosis/apoptosis. Herein, nanozymes (PPy-Mg NPs) were synthesized through coordination reaction between polypyrrole nanoparticles (PPy NPs) and magnesium ions. The enriched PPy-Mg NPs were explored as active anti-inflammatory nanozymes for effective acute liver injury treatment. Enriched cationic PPy-Mg NPs in the liver effectively scavenged excess reactive oxygen and nitrogen species (RONS) and cell-free DNA. It is worth emphasizing that in the delayed AILI mouse models, bioactive PPy-Mg NPs not only reduced oxidative stress levels but also modulated multiple biological signaling pathways, such as NF-κB, Nrf2-Keap1, ferroptosis/apoptosis. The research indicates the possible medical application of PPy-Mg NPs nanozymes in efficient enrichment treatment for acute liver injury. Statement of Significance APAP-induced liver injury (AILI) can progress to acute liver failure (ALF), presenting a significant clinical challenge. Currently, N-acetylcysteine (NAC) is the only medication approved treatment; however, its efficacy in patients with advanced AILI remains unsatisfactory. Therefore, the development of new therapeutics for the treatment of AILI are crucial. This work developed a PPy-Mg NPs nanozyme for the efficient AILI treatment. PPy-Mg NPs provide constructive applications of polypyrrole-based nanozymes for AILI treatment, which covers upstream metabolic rescue (RONS scavenging), necrotic debris clearance (cfDNA removal), and residual cell protection (ferroptosis inhibition) across temporal and spatial dimensions, holds potential for extending therapeutic windows and enhancing protection rates in high-risk/complex cases.
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ACS Applied Materials & Interfaces
·
2026
·
IF 7.8
Yang Hongqun,Li Longhao,Niu Mu,Wang Hong,Zhang Yingwei
The development of highly sensitive and reliable biosensors is essential for the early diagnosis and therapeutic monitoring of cancer. Herein, we report a dual-mode fluorescence–colorimetric biosensing platform for the ultrasensitive detection of miRNA-21, integrating AuNP-supported dual-stage rolling circle amplification (RCA) with an RCA-generated multipedal binding-and-cleavage mechanism. The sensing hierarchy is initiated by target-dependent activation of the primary RCA on the gold nanoparticle (AuNP) surface, producing long DNA strands with multiple repeated recognition domains that function as numerous walker “legs” and form a DNA-rich nanogel matrix. Through these repeated domains, the RCA-generated multipedal walker repeatedly and multivalently binds fluorophore-labeled hairpin substrates on the AuNP surface, thereby facilitating APE1-mediated substrate cleavage and fluorescence recovery. The APE1-generated cleavage fragments subsequently initiate the secondary RCA following the addition of the T2 template. The two successive RCA reactions ultimately produce an Au@RCA DNA nanogel containing abundant G-quadruplex/hemin DNAzymes that catalyze a visually detectable color change. By integrating the multipedal walker-mediated multivalent binding-and-cleavage process with dual-stage RCA, the platform enables dual-mode fluorescence–colorimetric detection with efficient signal amplification and low background, achieving limits of detection of 9.854 and 790 fM for the fluorescence and colorimetric modes, respectively. Furthermore, the two complementary outputs provide independent signal readouts for cross-validation. The biosensor demonstrated excellent specificity and distinguished the endogenous responses of MCF-7, HeLa, and HEK293T cell lysates. These results support the potential of the platform for ultrasensitive tumor-associated biomarker detection, while the cell-lysate experiments represent a preliminary proof-of-concept evaluation in complex biological matrices.
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TALANTA
·
2026
·
IF 6.7
Linna Li,Yandi Hui,Zhen Li,Xingtong Liu,Rauf Ayesha,Lei Tian,Fujun Yao,Xiaofeng Kang,Yanli Guo
Adenosine phosphates (ATP, ADP, and AMP) play critical roles in cellular processes, yet their simultaneous detection remains challenging due to high structural similarity. To overcome this limitation, we developed a nanopore-aptamer sensing platform capable of tracking single-molecule binding dynamics in real time. This method leverages the specific interactions between aptamers and each adenosine phosphate, enabling discrimination among ATP, ADP, and AMP at the single-molecule level. We demonstrate the high sensitivity and selectivity of this approach, which supports high-throughput analysis even in complex matrices. The platform was successfully applied to quantify ATP, ADP, and AMP in human serum, with excellent recovery rates (85.33%-97.53%) and precision (RSD < 7.03%), demonstrating its practical utility for multiplexed detection in biologically relevant environments. This work provides a powerful strategy for precise metabolite analysis and facilitates new studies on nucleotide-dependent pathways.
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ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Jiacheng Dong,Yu Zhang,Zhongyu Yang,Yanhua Rao,Yueyue Feng,Liying Huang,Shiwei Xu,Guofeng Wang,Nannan Liu
Endocrine-disrupting chemicals, particularly estradiol (E2), pose significant risks to environmental and human health due to their persistence and bioactivity. In this study, we developed a highly sensitive and label-free electrochemical sensor for E2 detection, based on a glass micropipette (GMP) filled with polydopamine nanotubes (PDA-NTs) and coupled with a small molecule-induced DNA strand dissociation strategy. PDA-NTs self-assemble into a densely packed structure at the tip of the GMP, providing abundant binding sites for E2 aptamer (Apt) immobilization and achieving the first signal amplification, which yields a detection limit of 1.29 pM. To further enhance sensitivity, a partially complementary DNA (cDNA) strand was hybridized with the aptamer to form an Apt-cDNA duplex. Upon E2 binding, the Apt in the Apt-cDNA duplex undergoes a conformational change that releases the sterically large cDNA, resulting in a significant increase in ionic current and enabling the second signal amplification. This second amplification improves the detection limit by 112-fold, reaching as low as 11.5 fM. The sensor can detect E2 in the detection range of 10 fM–10 nM and demonstrates high selectivity in complex mixed solutions such as fetal bovine serum (FBS) and environmental water. Beyond highly sensitive detection of E2, this dual-signal amplification also allows the detection of other small molecules through aptamer sequence replacement.
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