Journal of Macromolecular Science Part A-Pure and Applied Chemistry
·
2014
·
IF 0.74
Yan Xue,Dafu Wei,Anna Zheng,Yong Guan,Huining Xiao
Cationic star polymers as drug/gene carriers have attracted increasing attention. Herein, environmental sensitivities and star-shaped architecture were combined into a well-defined gene carrier system, and further amphiphilic modification was conducted. The thermo- and pH-sensitive behaviors of poly[N,N-(dimethyl amino)ethyl methacrylate] (PDMAEMA) star polymers before and after amphiphilic modification in aqueous solutions were investigated. The observed lower critical solution temperature (LCST) increased with increasing arm length due to the increase of hydrophilicity. A significant decrease of the LCSTs with increasing pH and ionic-strength of the solution was found. The incorporation of MMA and BA lipophilic moieties decreased the LCST of the cationic star polymer due to enhanced hydrophobic interaction. As a potential gene carrier, the performance of star polymers on binding DNA was primarily evaluated via particle size, zeta potential, and gel retardation measurements. All the complexes formed stable small particles with diameter of 100∼150 nm at N/P ratio higher than 3.0. The electrostatic screening effect and facilitation for the interaction of complexes with cell membrane induced by the incorporation of lipophilic units were expected to enhance the gene transfection efficiency.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2026
·
IF 4.6
Yaoxuan Wang,Haiyan Wang,Xianchuan Dong,Jing Sui,Feifei Zhang,Zonghua Wang
Here presents a sensing strategy for the detection of miRNA-155 based on a multi-amplification strategy, which involves nucleic acid recognition-induced multiple DNA release and Exonuclease III (Exo III)-assisted target recycling. Initially, cDNA-magnetic bead bioconjugates were employed to capture the target miRNA, triggering the release of two methylene blue (MB)-modified messenger DNAs (mDNAs). This step effectively doubles the amount of mDNAs-MB released, contributing to initial signal amplification. Subsequently, the target miRNA is recycled via Exo III-mediated cleavage, leading to the release of additional mDNAs-MB and further enhancing the signal. Finally, each capture probe (P1) is designed to hybridize with two mDNAs-MB, enabling dual signal recognition. As a result, the square wave voltammetry (SWV) measurements detected a significantly amplified MB peak current. The overall detection strategy integrates three synergistic amplification approaches: the release of multiple mDNAs, Exo III-assisted target miRNA recycling, and dual hybridization of mDNAs to P1. The miRNA-155, a recognized biomarker for breast cancer diagnosis, was used as the target to evaluate the biosensor's performance. The proposed electrochemical biosensor demonstrated a broad detection range from 0.1 pM to 1 mM and achieved a low detection limit of 0.026 pM, confirming its high sensitivity and potential applicability in cancer diagnostics.
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Acta Biomaterialia
·
2026
·
IF 10.4
Yu Liu,Ziwen Xiao,Yu Zhang,Qian Chen,Siyu Luo,Yaoqing Wang,Zhaohua Miao,Zhenchao Tao,Deyan Gong
Acute liver failure (ALF) can result from the progressive development of acute liver injury triggered by acetaminophen (APAP) overdose. The core mechanisms involve an imbalance in the antioxidant system and upregulation of the inflammatory response, which collectively induce ferroptosis/apoptosis. Herein, nanozymes (PPy-Mg NPs) were synthesized through coordination reaction between polypyrrole nanoparticles (PPy NPs) and magnesium ions. The enriched PPy-Mg NPs were explored as active anti-inflammatory nanozymes for effective acute liver injury treatment. Enriched cationic PPy-Mg NPs in the liver effectively scavenged excess reactive oxygen and nitrogen species (RONS) and cell-free DNA. It is worth emphasizing that in the delayed AILI mouse models, bioactive PPy-Mg NPs not only reduced oxidative stress levels but also modulated multiple biological signaling pathways, such as NF-κB, Nrf2-Keap1, ferroptosis/apoptosis. The research indicates the possible medical application of PPy-Mg NPs nanozymes in efficient enrichment treatment for acute liver injury. Statement of Significance APAP-induced liver injury (AILI) can progress to acute liver failure (ALF), presenting a significant clinical challenge. Currently, N-acetylcysteine (NAC) is the only medication approved treatment; however, its efficacy in patients with advanced AILI remains unsatisfactory. Therefore, the development of new therapeutics for the treatment of AILI are crucial. This work developed a PPy-Mg NPs nanozyme for the efficient AILI treatment. PPy-Mg NPs provide constructive applications of polypyrrole-based nanozymes for AILI treatment, which covers upstream metabolic rescue (RONS scavenging), necrotic debris clearance (cfDNA removal), and residual cell protection (ferroptosis inhibition) across temporal and spatial dimensions, holds potential for extending therapeutic windows and enhancing protection rates in high-risk/complex cases.
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NEW JOURNAL OF CHEMISTRY
·
2026
·
IF 2.6
Shilong Yang,Ying Tang,Mengyi Zhou,Wen Lu,Huayu Xue,Li Xu
Amentoflavone (Ame) is a kind of precious biflavonoids with various bioactivities. To further develop and use Ame, Ame was extracted and purified from ginkgo biloba leaves, and Ame complexes with metal ions (Mn 2+ /Fe 3+ /Co 2+ /Ni 2+ /Cu 2+ /Zn 2+ ) were first synthesized. The structures of Ame complexes were characterized by UV/Vis, FT-IR, HRMS and DFT calculation. The antioxidant capacity was evaluated by pyrogallol autoxidation and ABTS methods. DNA binding affinity were explored via fluorescence method and molecular docking. The structural analysis indicated the stoichiometric ratios of Ame and Mn 2+ /Co 2+ /Ni 2+ /Cu 2+ /Zn 2+ were 1:1, the chelation site was between 5''-hydroxyl and 4''-carbonyl. While the stoichiometric ratio of Ame and Fe 3+ was 2:2, and the chelation site was between 5''-hydroxyl and 4''-carbonyl, 5-hydroxyl and 4-carbonyl. The investigation of pyrogallol autoxidation and ABTS methods demonstrated Ame complexes had superior scavenging activities on O 2 -• and ABTS + • free radicals. What's more, scavenging free radical activity of some Ame complexes was stronger or similar comparing with VC. The results of fluorescence method indicated Ame complexes had stronger DNA binding affinity with intercalation mode. Molecular docking study confirmed Ame and its complexes interacted with DNA via Van der Waals, conventional hydrogen bond and π-π stacked effect. These results demonstrated bioactivities of Ame could be enhanced via forming complexes with metal ions and provided theoretical knowledge for biflavonoids acting as potential drug, dietary supplement and cosmetics.
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ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Jiacheng Dong,Yu Zhang,Zhongyu Yang,Yanhua Rao,Yueyue Feng,Liying Huang,Shiwei Xu,Guofeng Wang,Nannan Liu
Endocrine-disrupting chemicals, particularly estradiol (E2), pose significant risks to environmental and human health due to their persistence and bioactivity. In this study, we developed a highly sensitive and label-free electrochemical sensor for E2 detection, based on a glass micropipette (GMP) filled with polydopamine nanotubes (PDA-NTs) and coupled with a small molecule-induced DNA strand dissociation strategy. PDA-NTs self-assemble into a densely packed structure at the tip of the GMP, providing abundant binding sites for E2 aptamer (Apt) immobilization and achieving the first signal amplification, which yields a detection limit of 1.29 pM. To further enhance sensitivity, a partially complementary DNA (cDNA) strand was hybridized with the aptamer to form an Apt-cDNA duplex. Upon E2 binding, the Apt in the Apt-cDNA duplex undergoes a conformational change that releases the sterically large cDNA, resulting in a significant increase in ionic current and enabling the second signal amplification. This second amplification improves the detection limit by 112-fold, reaching as low as 11.5 fM. The sensor can detect E2 in the detection range of 10 fM–10 nM and demonstrates high selectivity in complex mixed solutions such as fetal bovine serum (FBS) and environmental water. Beyond highly sensitive detection of E2, this dual-signal amplification also allows the detection of other small molecules through aptamer sequence replacement.
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高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2012
·Vol. 16
·期 8
·2837-2843
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IF 2.131
Meng Xiaomeng,Yin Huanshun,Xu Minrong,Ai Shiyun,Zhu Jianying
Nonylphenol (NP) was determined using electrochemical method based on ionic liquid-functionalized graphene nanosheet modified electrode. The fabricated electrode was characterized by electrochemical impedance spectroscopy. The different influence parameters, such as pH value, scan rate, accumulation potential, and time, were investigated. Under the optimum conditions, the linear relationship between peak current value and concentration of NP was obtained with differential pulse voltammetry in two ranges from 0.5 to 30 μM and 30 to 200 μM with the detection limit of 0.058 μM ( S/N = 3). Moreover, this modified electrode was applied to NP determination in water and soil samples with the recoveries from 94.8 to 104 %, which showed the method could be applied to determine NP in environment. In addition, to explore the damage to DNA, the interaction between NP and DNA was investigated with the association constant ( β ) of 0.4867 and the Hill coefficient ( m ) of 3.75 × 10 5 M −1 , respectively.
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TALANTA
·
2026
·
IF 6.7
Linna Li,Yandi Hui,Zhen Li,Xingtong Liu,Rauf Ayesha,Lei Tian,Fujun Yao,Xiaofeng Kang,Yanli Guo
Adenosine phosphates (ATP, ADP, and AMP) play critical roles in cellular processes, yet their simultaneous detection remains challenging due to high structural similarity. To overcome this limitation, we developed a nanopore-aptamer sensing platform capable of tracking single-molecule binding dynamics in real time. This method leverages the specific interactions between aptamers and each adenosine phosphate, enabling discrimination among ATP, ADP, and AMP at the single-molecule level. We demonstrate the high sensitivity and selectivity of this approach, which supports high-throughput analysis even in complex matrices. The platform was successfully applied to quantify ATP, ADP, and AMP in human serum, with excellent recovery rates (85.33%-97.53%) and precision (RSD < 7.03%), demonstrating its practical utility for multiplexed detection in biologically relevant environments. This work provides a powerful strategy for precise metabolite analysis and facilitates new studies on nucleotide-dependent pathways.
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高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·Vol. 415
·期 12
·2271-2280
·
IF 4.3
An Yaqian,Yu Zhiqi,Liu Di,Han Lirong,Zhang Xian,Xin Xuelian,Li Cuiping
The detection of methyltransferase (MTase) activity is of great significance in methylation-related disease diagnosis and drug screening. Herein, a HpaII-assisted and linear amplification-enhanced exponential amplification strategy is proposed for sensitive and label-free detection of M.SssI MTase activity. The P1 probe contains self-complementary sequence 5′-CTAGCCGGCTAG-3′ at 3′-terminal. After denaturation and annealing, P1 probes hybridize with itself to generate P1 duplexes. M.SssI MTase induces methylation of cytosine at 5′-CG-3′ in P1 duplexes, and thus, HpaII fails to cleave at 5′-CCGG-3′ due to methylation sensitivity, leaving P1 duplex intact. Then, these intact P1 duplexes are extended along 3′-terminal through Vent (exo − ) DNA polymerase to generate dsDNA, which is recognized and nicked at the recognition sites by Nt.BstNBI, releasing two copies of primer X. Primer X hybridizes with X′ at the amplification template T1 (X′-Y′-X′) and then serves as primers to trigger the exponential amplification reaction (EXPAR). The point of inflection (POI) values of real-time fluorescence curves is linearly correlated with the logarithm of M.SssI MTase concentration in the range of 0.125 \(-\) 8 U mL −1 with a low detection limit of 0.034 U mL −1 . In the absence of M.SssI, P1 duplexes are cut by HpaII and separated into ssDNA under the executed temperature of EXPAR and thus unable to trigger the amplification. The strategy provides good selectivity against other types of MTases and protein and is able to detect M.SssI activity in human serum. Furthermore, the analytical method has the generality and can be extended to the analysis of other types of DNA MTases. Graphical Abstract
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高影响因子
MACROMOLECULAR RAPID COMMUNICATIONS
·
2023
·Vol. 44
·期 23
·2300192
·
IF 4.6
Zhaoxu Wang,Qiujun Liu,Qian Liu,Hao Qi,Yuesheng Li,Dong-Po Song
Microspheres bearing large pores are useful in the capture and separation of biomolecules. However, pore size is typically poorly controlled, leading to disordered porous structures with limited performances. Herein, ordered porous spheres with a layer of cations on the internal surface of the nanopores are facilely fabricated in a single step for effective loading of DNA bearing negative charges. Triblock bottlebrush copolymers (BBCPs), (polynorbornene- g -polystyrene)- b -(polynorbornene- g -polyethylene oxide)- b -(polynorbornene- g -bromoethane) (PNPS- b -PNPEO- b -PNBr), are designed and synthesized for fabrication of the positively charged porous spheres through self-assembly and in situ quaternization during an organized spontaneous emulsification (OSE) process. Pore diameter as well as charge density increase with the increase of PNBr content, resulting in a significant increase of loading density from 4.79 to 22.5 ng µg −1 within the spheres. This work provides a general strategy for efficient loading and encapsulation of DNA, which may be extended to a variety of different areas for different real applications.
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TALANTA
·
2023
·
IF 6.1
Jinpeng Liu,Yanhui Bi,Wenjun Tai,Yong Wei,Qiang Zhang,Anna Liu,Qiongzheng Hu,Li Yu
Due to the serious risks of lead pollution to human health, it plays a great role in constructing a simple, inexpensive, portable, and user-friendly strategy for Pb 2+ detection in environmental samples. Herein, a paper-based distance sensor is developed to detect Pb 2+ assisted with the target-responsive DNA hydrogel. Pb 2+ can activate DNAzyme to cleave its substrate strand, which results in the hydrolysis of the DNA hydrogel. The released water molecules trapped in the hydrogel can flow along the patterned pH paper due to the capillary force. The water flow distance (WFD) is significantly influenced by the amount of water released from the collapsed DNA hydrogel triggered by the addition of various Pb 2+ concentrations. In this way, Pb 2+ can be quantitatively detected without using specialized instruments and labeled molecules, and the limit of detection (LOD) of Pb 2+ is 3.0 nM. Additionally, the Pb 2+ sensor works well in lake water and tap water. Overall, this simple, inexpensive, portable, and user-friendly method is very promising for quantitative and in-field detection of Pb 2+ with excellent sensitivity and selectivity .
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TALANTA
·
2023
·
IF 6.1
Ying Yu,Zhijia Gu,Maokang Li,Yongfu Pu,Ariadni Geballa Koukoula,Jeroen Peters,Ying Yang,Xiaodi Hong
DNA aptamer superparamagnetic photonic crystals (DSPCs), enriched with a highly selective cytosine-rich mismatched single-stranded DNA aptamer (CRDA), were successfully employed in a novel visual detection strategy for the detection of silver ions (Ag + ). The technologies of superparamagnetic colloidal nanospheres (SCNs), DNA aptamer, and photonic crystals were combined to fabricate DPSCs. The aptamer was immobilized via electrostatic adsorption with amino groups that were chemically introduced on the surface of the SCNs, forming D–NH–SCNs. The detection is achieved by forming an Ag + complex (C–Ag + –C) between Ag+ and D–NH–SCN. The DSPCs assembled under a magnetic field by D–NH–SCNs effectively detected Ag + in the range of 1 μg/L to 5 mg/L, corresponding to the critical concentration range for heavy metals in drinking water . During the detection, the DSPC exhibited a wavelength blueshift from 652.8 nm to 626.4 nm (26.4 nm), as well as changes in reflection intensity. Notably, when detecting Ag+, a change in DSPC color from orange to yellow was observed. In summary, the developed visual detection material facilitates direct Ag + sensing. In the future, different DNA aptamers will be modified further to detect various targets in the fields of medicine, environmental monitoring, and food safety.
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高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Zhihong Yan,Hong Li
A Cobalt (III) trisphenanthroline complex ([Co(phen)3] 3+ ) has been adsorption assembled on a glassy carbon electrode (GCE) modified with DNA decorated with graphene oxide (GO) by multiple sweep voltammetry. In the present protocol, GO is served as a functional unit of DNA to greatly improve the efficient immobilization of DNA on the GC electrode and also offer a favorable microenvironment to keep the DNA structure of double-stranded (dsDNA), which facilitates the intercalation of redoxactive [Co(phen)3] 3+ . The evidences from scanning electron microscope, X-ray diffraction, cyclic voltammetry, electrochemical impedance spectroscopy, emission spectroscopy and differential pulse voltammetry reveal that [Co(phen)3] 3+ can be assembled on the surfaces of GO-decorated dsDNA driven by electrostatic attraction, intercalation and π-stacking interaction. Furthermore, the highly stable [Co(phen)3] 3+ /GO-dsDNA/GCE is used to the highly selective determination of 6- mercaptopurine (6-MP) in the human blood with satisfactory. The reduction peak current was linear to 6-MP concentration in the range from 5×10 -8 to 2×10 -6 M 6-MP, and the detection limit is 1.5×10 -8 M ( S / N = 3)
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