ACS Applied Bio Materials
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2026
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IF 4.7
Hao Lyu,Niping Deng,Jiaqi Wang,Dandan Dai,Ziyang Sun,Chengchen Guo
The programmed polarization of macrophages, which exhibit remarkable plasticity from pro-inflammatory (M1) to anti-inflammatory (M2) phenotypes, serves as a key driver of skin wound healing. However, dysregulated macrophage polarization toward a dominant M1 phenotype can induce excessive inflammation and hinder wound healing. Current therapeutic strategies to promote M2 polarization, such as cytokines, anti-inflammatory drugs, and stem cell therapies, have limited effectiveness, complex manufacturing processes, and potential toxicity. Here, we report the development of mannose- and sulfonic acid-modified silk fibroin (SF) that are bioactive and promote M2 polarization by activating the MR-ERK/STAT6 signaling axis. In vitro studies showed increased expression of CD206 and anti-inflammatory gene markers, confirming their ability to regulate macrophage polarization without additional therapeutic agents. Moreover, the mannose- and sulfonic acid-modified SF films, used as wound dressings, enhanced wound healing by promoting M2 macrophage polarization, angiogenesis, collagen deposition, and wound closure. These findings highlight the potential of chemically modified SF as bioactive materials for immune modulation and tissue regeneration.
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Nature Energy
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2026
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IF 70.1
Zhao Qiangqiang,Zhang Bingqian,Hui Wei,Gao Kun,Ji Hongpei,Sun Xiuhong,Feng Xiaopeng,Peng Cheng,Wang Kaiyu,Gao Caiyun,Zhang Chenyang,Wang Han,Zhang Qi,Su Zhenhuang,Wang Xiao,Wang Li,Pang Shuping,De Wolf Stefaan,Wang Kai
Incorporating two-dimensional (2D) perovskite phases at the interfaces of three-dimensional (3D) perovskite solar cells improves device performance. These 2D structures form in the bulk and at interfaces of the perovskite film by adding long-chain ammonium salts into the perovskite, but achieving them exclusively at the buried interface remains challenging. Here we sequentially graft thioglycolic acid and oleylamine onto SnO 2 nanoparticles. The strong chemical bond between thioglycolic acid and oleylamine ensures that cation exchange with formamidinium iodide, a perovskite precursor, occurs only during thermal annealing, creating a 2D/3D perovskite structure solely at the bottom interface. This localized 2D layer accelerates 3D phase formation, enhances perovskite crystallization and reduces defect concentration at the interface by over tenfold. The resulting solar cells achieve power conversion efficiencies of 26.19% (certified 26.04%, 0.09 cm 2 ), 23.44% (aperture area of 21.54 cm 2 , certified 22.68%) and 22.22% (aperture area of 64.80 cm 2 ), respectively.
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CHEMICAL ENGINEERING JOURNAL
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2026
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IF 12.5
Yuanyuan Shen,Xuerui Zang,Haibin Zhao,Fei Xu,Jiajia Wu,Peng Wang
Marine biofouling presents substantial economic and ecological challenges to maritime operations and the broader marine industry, highlighting the urgent need for effective antifouling technologies. In this study, we present a robust and straightforward strategy to fabricate liquid-like antifouling coatings (CAP–PMHS) by grafting natural capsaicin (CAP) onto the surface of poly(methylhydrosiloxane) (PMHS) through a one-step hydrosilylation reaction. The resulting eco-friendly coating significantly reduces bacterial adhesion by 96.3 ± 0.4% within 10 min. Mechanistically, the CAP–PMHS surface inhibits the initial adhesion and biofilm formation of Pseudomonas aeruginosa (microfouling organism) by disrupting bacterial chemosensory system and associated metabolic pathways. Furthermore, CAP–PMHS markedly suppresses the sensory responses of Crassostrea gigas (macrofouling organism) by targeting the sensory organs (foot region). Specifically, it effectively inhibits larval settlement by interfering with the chemosensitive transient receptor potential melastatin subfamily member 8 (TRPM8) channel, which is highly expressed in foot tissues of pediveligers. This simple yet effective design strategy, combined with molecular-level insights into sensing and adhesion mechanisms of fouling organisms, opens new pathways for developing non-lethal multifunctional antifouling materials with cross-species applicability. It further establishes a theoretical foundation for antifouling through bio-behavior modulation, enabling sustainable applications in marine antifouling, drag reduction, and biomedical engineering.
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APPLIED SURFACE SCIENCE
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2026
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IF 6.6
Hanzhong Cui,Yuxi Chen,Meilin Zhang,Zhou Yang,Luyu Ji,Jin Zhang
Graphene film is a layered material with high thermal and electrical conductivity. Its properties depend on the alignment and packing of graphene sheets. In this work, capillary slit assisted self-assembly strategy was developed to fabricate freestanding graphene film with improved orientation and structural uniformity. A suspension of graphene oxide (GO) and commercial graphene (CG) was injected into a slit mold, where capillary forces and micro-convective flow promoted the alignment and dense stacking of graphene sheets during solvent evaporation. The resulting films were thermally reduced to produce reduced GO/CG composites, denoted as SrGO/G (with slit) and rGO/G (without slit). Structural characterization revealed that SrGO/G possesses a smoother surface, a more ordered layered structure, and a higher Herman’s orientation factor (0.91) compared to rGO/G (0.86). These structural advantages result in a higher in-plane thermal conductivity of 102.86 W m −1 K −1 for SrGO/G, compared with 26.74W m −1 K −1 for rGO/G. This enhancement is attributed to the more ordered and densely packed graphene sheets, which facilitate phonon transport along the in-plane direction. These results demonstrate that slit-assisted assembly is a simple and effective strategy to control graphene sheet alignment and enhance thermal properties.
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Food Chemistry-X
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2026
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IF 9.2
Hong Wang,Li Wan,Yu Wang,Yan Li,Wenli Zhao,Wenjuan Liang
Coffee “husks”, as primary by-products whose inadequate disposal causes environmental pollution, are rich in bioactive ingredients. The in-depth exploration and practical application of these by-products remain insufficient. A comprehensive investigation was performed with the combination of LC-MS n -IT-TOF analysis, antioxidant and xanthine oxidase (XO) inhibitory assays. A total of 55 main characteristic compounds were identified and their detailed mass spectrometry cleavage patterns were elucidated. Chlorogenic acid analogs as key bioactive constituents comprised over 66% of the identified phenols, which spontaneously bind to XO, with binding energy ranging from −4.46 to −3.32 kcal/mol. The antioxidant activities of di-substituted chlorogenic acids were significantly higher than those of mono-substituted molecules. Flavonoids from the “husks” with XO inhibitory activities were attributed to the flavone skeleton, in which the presence of a stable chromone group was vital. These results provided novel perspectives for discovery of XO inhibitors from widely discarded natural by-products like coffee “husks”.
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BIOMACROMOLECULES
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2026
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IF 5.4
Pengxiao Liu,Kai Wei,Yong Liu,Liangjiu Bai,Hou Chen,Wenxiang Wang,Lixia Yang,Huawei Yang,Donglei Wei
Soilless cultivation relies on hydrogel matrices for water and nutrient management, but conventional hydrogels lose performance at low temperature and show unstable sustained release. Here this study develops an antifreeze sustained-release hydrogel (P-GTCH) that integrates l-proline (L-P) with a humic-acid-modified cellulose nanocrystal nanocomposite (CNCs-HA) for nanoenabled controlled release. L-P suppresses ice nucleation via hydrogen bonding, while CNCs-HA boosts HA loading and enables controlled transport within the cross-linked network. HA shows slow, continuous release governed by Fickian diffusion, achieving 96.7% cumulative release over 12 days at 0 °C and mitigating poor fertilizer release in cold environments. P-GTCH provides mechanical support for plant roots, and the synergistic effects of L-P and HA maintain lettuce germination above 86% at 0 °C while promoting root growth. This biobased, biodegradable platform is scalable for low-temperature soilless cultivation.
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TALANTA
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2026
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IF 6.7
Jingjing Yin,Yuehua Wang,Shanshan Zhu,Xiaoxue Liu,Yujie Sun,Hong Yang,Yifei Ma,Jinhua Liu
The development of wavelength-tunable fluorescence immunoassays for sensitive biomarker detection remains a significant challenge in clinical diagnostics. In this work, we present an HRP-induced in situ fluorogenic immunoassay platform based on the rapid reaction between p-phenylenediamine (PPD) analogues and 6-hydroxyquinoline (6-Hy). The proposed mechanism involves HRP-catalyzed generation of hydroxyl radicals from H 2 O 2 , which subsequently oxidize PPD to form fluorescent copolymer nanoparticles (FCNPs) with 6-Hy. By strategically modifying the PPD structure with various substituents, we achieved discrete and tunable emission of FCNPs, with maxima spanning from 380 to 600 nm (Δλ ≈ 220 nm). Using cardiac troponin I (cTnI) as a model antigen and zinc-coordination nanoparticles (ZnNPs) as carriers, the immunoassay demonstrated exceptional performance, with a wide dynamic range (0.5-125 ng/mL) and an ultralow detection limit (0.17 ng/mL) for cTnI. The platform was successfully validated in human serum samples, showing excellent correlation with clinical standards. This work not only establishes a novel strategy for wavelength-programmable immunoassays but also provides a versatile platform for advancing biomarker detection in clinical diagnostics.
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ANALYTICAL CHEMISTRY
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2026
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IF 6.7
Qianqian Cui,Huijuan Yang,Zizhe Li,Yexuan Mao,Meng Dang,Xianqing Huang,Lianjun Song,Tong Bu,Xiya Zhang
Benzene ring-based spacer arms have great application potential in hapten design for preparing high-affinity antibodies against small molecules. However, current studies have not systematically reported how different benzene ring spacer arm positions in the hapten regulate the antibody affinity to the coating antigen. In this work, 12 unique coating haptens with ortho, meta, and para benzene ring spacer arm positions were synthesized and coupled to carrier proteins to form coating antigens. This was done using 2-nitrobenzaldehyde derivatized semicarbazide (linear molecule), 3-amino-2-oxazolidinone (cyclic molecule), 1-aminohydantoin (cyclic molecule), and 3-amino-5-morpholinomethyl-2-oxazolidinone (bicyclic molecule) as targets to determine the influence of the spacer position at the benzene ring on the antibody affinity for the coating antigen. First, we evaluated the performance of eight prepared monoclonal antibodies (mAbs) by an indirect enzyme-linked immunosorbent assay (iELISA), indirect competitive ELISA (icELISA), and lateral flow immunoassay (LFA). Moreover, the interaction forces between antibodies and different coating haptens were examined by molecular docking to investigate the aforementioned regulatory mechanisms. Specifically, we confirmed that in coating hapten design (linear/cyclic molecule), the position (meta, para) of benzene ring spacer arms used for carrier protein conjugation enhanced antigen–antibody recognition via π–π or π–alkyl interactions, thereby modulating antibody affinity toward the derivatized compounds. Although the benzene ring spacer arm minimally contributed to antigen–antibody recognition in complex small molecules (e.g., bicyclic compounds), its primary role was to maintain a critical hapten–carrier protein distance and prevent steric hindrance. Finally, highly sensitive LFAs for detecting nitrofuran residues in fish and shrimp were developed, providing a versatile platform that can be extended to other chemical compounds.
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Cellular and Molecular Gastroenterology and Hepatology
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2026
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IF 7.4
Lu Chen,Yuxuan Yang,Yong Zhang,Xiangyu Yang,Xinmei Zhang,Lin Chen,Wenqing Li,Siyuan Zhang,Ruiyan Xiong,Jie Wang,Zhipeng Xu,Zhi-Yuan Zhang,Cailong Pan,Minjun Ji
Background & Aims Tuft cells, a type of epithelial cell in the gut, play a pivotal role in regulating type 2 immunity and maintaining the gut barrier. However, their role in cognitive impairments remains unclear. Methods We compared behavioral performance between male tuft cell-absent mice (Pou2f3 −/− ) and their wild-type littermates (WT). We analyzed gut microbiota using fecal 16S rRNA, measured gut permeability via FITC-dextran assay, and detected CD4 + -T cells and type 2 innate lymphoid cells by flow cytometry in both genotypes. Co-housing and fecal microbiota transplantation (FMT) experiments were conducted to explore the role of gut microbiota in cognitive diseases. Single-cell RNA sequencing and fluorescence imaging were used to examine tuft cell changes in the colon of WT and Alzheimer's disease (AD) model mice. Colonic organoids were used to assess the effect of β-amyloid on tuft cell differentiation. Succinic acid, a promoter of tuft cells, was administered, and tuft cell-deficient AD mice were generated to evaluate its impact on behavior and gut homeostasis. Results Increased gut permeability, immune imbalance, neuroinflammation, and cognitive dysfunction occurred in 10-month-old mice lacking tuft cells. These alterations were mediated by gut microbiota, evidenced by shifts in microbiota composition and abundance, and supported by co-housing and FMT experiments. AD model mice had fewer tuft cells and impaired type 2 immunity in the gut, potentially because of β-amyloid inhibiting tuft cell differentiation. Succinic acid, a tuft cell activator, restored cognitive function and gut homeostasis in AD mice. Conclusion Tuft cells may be necessary for maintaining gut homeostasis in cognitive disorders.
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Cell Communication and Signaling
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2026
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IF 8.9
Qiu Yueping,Liu Yijie,Ding Haiying,Xin Wenxiu,Lu Shuanghui,Hu Yan,Chen Jing,Weng Qinjie,Wang Jiajia,Fang Luo
Osimertinib is a first-line treatment for advanced non-small cell lung cancer with epidermal growth factor receptor (EGFR)-sensitive mutations; however, the severe hepatotoxicity of osimertinib is a challenge that has attracted attention. In this study, we found that osimertinib directly caused hepatocyte damage, with cells showing morphological features of vacuolization after treatment. Using transmission electron microscopy and other analytical techniques, we found that excessive activation of the autophagy-lysosome pathway was the main cause of cell vacuolation. Mechanistically, osimertinib causes dephosphorylation of transcription factor EB (TFEB), leading to its translocation into the nucleus to exert transcriptional regulatory functions. TFEB in the nucleus induces autophagy and lysosome biogenesis, thereby causing excessive activation of the autophagy-lysosome pathway subsequently leading to vacuolation-associated cell death. Through this study, we have identified S-adenosyl-L-methionine as a clinical hepatoprotective agent that could mitigate osimertinib-induced hepatocyte damage by inhibiting TFEB activation. Collectively, our findings not only lay the groundwork for addressing the clinical challenges posed by osimertinib hepatotoxicity but also broaden our understanding of drug-induced hepatocyte injury, thereby offering a novel research pathway for the study of drug-induced liver injury.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2026
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IF 3.8
Lu Xueyun,Huo Xiaohe,Tian Yafei,Xiao Dan,Zhou Cuisong
The development of feasible, low-cost, and long-persistent chemiluminescence (CL) systems is highly desirable for enhancing detection reproducibility and accuracy. Here, we present a space-confined CL system based on the LHC@G capsules, which are prepared via a one-pot ultrasound-assisted method to co-encapsulate glucose oxidase (GOx), hemin (a peroxidase mimic), and luminol within an oil-in-water structure. The LHC@G capsules are used for producing glow-type CL emission. Specifically, glucose is catalyzed by GOx on the capsule surface, yielding H 2 O 2 . The H 2 O 2 then diffuses into the oil core and undergoes hemin-catalyzed in situ blue emission of luminol. The LHC@G capsule-based CL system exhibits intensive and prolonged blue emission for over 1500 s, with a nearly threefold enhancement in CL intensity compared to that of luminol solution. Mechanistic studies reveal that the excellent CL performance stems from a highly efficient cascade reaction and regulated diffusion. The proposed capsule CL system exhibits excellent reproducibility and storage stability, and has been successfully developed for simple, fast, and sensitive visual glucose detection with a LOD of 1.21 μM. This work provides a convenient strategy for constructing long-persistent and accurate CL systems in the field of point-of-care testing (POCT). Graphical abstract
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Materials Today Bio
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2026
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IF 11
Hongyun Xuan,Zihao Liu,Keyu Lu,Yao Chen,Haonan Gu,Biyun Li,Jingjun Sun,Yan Jin,Yumin Yang,Huihua Yuan
Diabetic wound repair remains a formidable clinical challenge due to impaired healing and heightened infection risks associated with conventional sutures. To address these limitations, this study introduces a novel injectable, self-healing, and antibacterial polysaccharide hydrogel (PGHAA), synthesized from borated peach gum and oxime-modified hyaluronic acid. By leveraging dynamic boronic ester bonds and metal coordination, PGHAA demonstrates enhanced tissue adhesion, self-healing capabilities, and antibacterial activity with immunomodulatory capacity to reprogram chronic wounds into a regenerative state. The incorporation of arginine as a cross-linking agent further improves both biocompatibility and functional performance. In vitro and in vivo evaluations indicate that PGHAA facilitates rapid hemostasis, robust tissue adhesion, and macrophage polarization toward a pro-regenerative phenotype, resulting in accelerated diabetic wound healing. Using an in vivo study with chronic diabetic skin we demonstrated that PGHAA induced wound healing via modulation of IL-17/NF-κB signaling—a pathway repurposed from its classical inflammatory role to drive tissue regeneration. This work redefines diabetic wound therapy by introducing a first-in-class hydrogel that leverages immune modulation for tissue repair, offering a transformative solution for regenerative medicine.
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