BIOCONJUGATE CHEMISTRY
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2017
·
IF 4.818
Ludan Yue,Jinlong Wang,Zhichao Dai,Zunfu Hu,Xue Chen,Yafei Qi,Xiuwen Zheng,Dexin Yu
Multifunctional nanotheranostic agents have been highly commended due to the application to image-guided cancer therapy. Herein, based on the chemically disordered face centered cubic (fcc) FePt nanoparticles (NPs) and graphene oxide (GO), we develop a pH-responsive FePt-based multifunctional theranostic agent for potential in vivo and in vitro dual modal MRI/CT imaging and in situ cancer inhibition. The fcc-FePt will release highly active Fe ions due to the low pH in tumor cells, which would catalyze H2O2 decomposition into reactive oxygen species (ROS) within the cells and further induce cancer cell apoptosis. Conjugated with folic acid (FA), the iron platinum-dimercaptosuccinnic acid/PEGylated graphene oxide-folic acid (FePt-DMSA/GO-PEG-FA) composite nanoassemblies (FePt/GO CNs) could effectively target and show significant toxicity to FA receptor-positive tumor cells, but no obvious toxicity to FA receptor-negative normal cells, which was evaluated by WST-1 assay. The FePt-based multifunctional nanoparticles allow real-time monitoring of Fe release by T2-weighted MRI, and the selective contrast enhancement in CT could be estimated in vivo after injection. The results showed that FePt-based NPs displayed excellent biocompatibility and favorable MRI/CT imaging ability in vivo and in vitro. Meanwhile, the decomposition of FePt will dramatically decrease the T2-weighted MRI signal and increase the ROS signal, which enables real-time and in situ visualized monitoring of Fe release in tumor cells. In addition, the self-sacrificial decomposition of fcc-FePt will be propitious to the self-clearance of the as-prepared FePt-based nanocomposite in vivo. Therefore, the FePt/GO CNs could serve as a potential multifunctional theranostic nanoplatform of MRI/CT imaging guided cancer diagnosis and therapy in the clinic.
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BIOMACROMOLECULES
·
2018
·
IF 5.738
Weitong Sun,Xiaoyan Chen,Chao Xie,Yong Wang,Liteng Lin,Kangshun Zhu,Xintao Shuai
Drug resistance, developed through multiple mechanisms, is a major hindrance to successful chemotherapy of tumor. Combination therapy of chemotherapeutic drugs and siRNA represents an emerging strategy which may improve anticancer effect by synergistic actions. In this study, triblock copolymer of poly(ethylene glycol)-block-poly(l-lysine)-block-poly aspartyl (N-(N′,N′-diisopropylaminoethyl)) (PEG-PLL-PAsp(DIP)) was synthesized for the first time to enable the codelivery of BCL-2 siRNA and DOX. The system is supposed to not only bypass drug efflux but also down-regulate the antiapoptotic gene and consequently confronting against chemoresistance as well. Moreover, the pH responsive ability of the codelivery system can prevent drug leakage during circulation and guarantee swift drug release at tumors. The codelivered siRNA serves to suppress the expression of antiapoptotic BCL-2 and hence sensitize the cancer cells to anticancer drugs and produce improved therapeutic effect. Consequently, the codelivery of BCL-2 siRNA and anticancer drug DOX serves as a promising strategy against drug resistance in chemotherapy.
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INTERNATIONAL IMMUNOPHARMACOLOGY
·
2025
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IF 4.7
Dake Huang,Ke Shen,Li Gui,Qiongqiong Cao,Fangmei Yu,Yunxia Lu
Given resveratrol's (RES) potential as an anti-aging agent, this study explored its ability to inhibit the NOD-, LRR-, and pyrin domain-containing protein 3 (NLRP3) inflammasome through the miR-217/SIRT1 axis in senescent endothelial cells. Male C57BL/6 mice were administered a customized regular diet supplemented with 0.04 % RES for 64 consecutive weeks. Subsequently, the effects of RES on senescent human umbilical vein endothelial cells (HUVECs) induced by H 2 O 2 were evaluated. Gene expression analyses were conducted following SIRT1 knockdown in HUVECs treated with H 2 O 2 and RES, or transfected with miR-217 mimics and inhibitors. The study found that RES reduced body weight, improved endothelial function, enhanced SIRT1 expression, and suppressed the expression of miR-217, NADPH oxidase 4 (NOX4), and NLRP3 in the senescent thoracic aorta. Additionally, RES significantly reduced the expression of NOX4, X-box binding protein 1 spliced (XBP1s), and reactive oxygen species (ROS) production in a dose-dependent manner in senescent HUVECs. SIRT1 knockdown resulted in the activation of the NLRP3 inflammasome by upregulating NOX4 and XBP1s expression, an effect that was partially attenuated by RES pretreatment. RES also suppressed the expression of miR-217 induced by H 2 O 2 . The miR-217 mimic facilitated cellular senescence; however, RES mitigated this effect, which was further amplified by the miR-217 inhibitor.
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APPLIED SURFACE SCIENCE
·
2025
·
IF 6.9
Zhicong Wei,Junnan Qu,Peilun Shen,Haokai Di,Yang Liu,Liuyang Dong,Yan Huan,Yanling Jin,Haiyun Xie,Qihao Gui,Dianwen Liu
Arsenopyrite, as the main carrier of precious metals and a companion mineral of sulfide ores, needs to be removed in advance before entering the metallurgy process to reduce its impact. The catalytic role of chalcopyrite in enhancing arsenopyrite oxidation under sulfate radical-based advanced oxidation processes (SR-AOPs) was systematically investigated. Through multi-scale characterization (XRD, SEM-EDS, XPS, FTIR, Tof-SIMS, EPR) and electrochemical analysis (OCP, CV, Tafel), this study demonstrates that chalcopyrite significantly accelerates arsenopyrite oxidation. Electrochemical analysis indicates that Chpy optimizes the electron transfer pathway and accelerates the oxidation process of pyrite. Surface analysis identified α-FeOOH/As–O oxide layers and Cu-As/S complexes as critical intermediates. The EPR and quenching experiments demonstrated that multiple activation pathways of chalcopyrite effectively promoted the free radical conversion rate of PDS and enhanced the oxidation efficiency of arsenopyrite. These findings provide mechanistic insights into chalcopyrite-driven arsenopyrite oxidation, offering a sustainable strategy for arsenic removal with reduced reagent consumption.
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JOURNAL OF FOOD SCIENCE
·
2026
·Vol. 91
·期 7
·e71261
·
IF 4.1
Eihab Hatem JadElrab,Muhammad Umair Khalid,Abdullah S. Seddiek,Dazhuang Liu,Musab Ali,Waleed Al-Ansi,Abdalbasit A. Mariod,Abdelaziz Elbarbary,Shaofeng Yuan,Hang Yu,Weirong Yao
This study developed a novel synergistic antibacterial system combining ε-poly-L-lysine (ε-PL) and oregano essential oil (OEO) as a natural, non-thermal preservation strategy for fruits. Among ε-PL combinations with seven different essential oils, OEO exhibited the strongest synergistic interaction, significantly enhancing antibacterial efficacy, compared to individual agents, likely through disruption of bacterial cell membranes and leakage of intracellular components. This effect was validated through integrated in vitro assays, in vivo application on strawberries, and in silico molecular docking analysis, which showed a binding energy of −7.8 kcal/mol. The ε-PL/OEO treatment reduced total viable counts to < 3.0 log CFU/g and extended shelf life by 5 days at room temperature. Furthermore, the treatment effectively preserved key quality parameters, including pH (2.69), titratable acidity (0.493%), total soluble solids (7.4 °Brix), and reducing sugars (0.772 g/100 g), while minimizing weight loss to < 2.0%. These findings demonstrate that the ε-PL/OEO combination represents a promising, sustainable alternative to synthetic preservatives, offering enhanced microbial safety and improved quality retention in perishable fruits.
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BIOORGANIC CHEMISTRY
·
2026
·
IF 5.1
Wenyu Xie,Ke Wu,Lin Zhang,Xiyu Feng,Shangshen Yang,Siyu Jia,Yutong Li,Xiaoming Wang
Hydrogen sulfide (H₂S) plays a crucial neuroprotective role in Parkinson's disease (PD). Cystathionine-β-synthase (CBS), a key enzyme involved in H₂S biosynthesis, exhibits expression deficiencies that are closely linked to PD progression. This suggests that enhancing the CBS–H₂S signaling axis to restore H₂S homeostasis may be a critical approach for preventing and treating PD. In the present study, the H₂S donor sulforaphane (SFN) was investigated to elucidate its neuroprotective mechanisms through activation of the CBS–H₂S axis. siRNA-mediated silencing of Nrf2 and CBS was employed to clarify the role of each in SFN's effects. Our findings demonstrate that SFN promotes CBS expression and H₂S synthesis, activates mitophagy to clear damaged mitochondria, reduces mitochondrial-derived reactive oxygen species (mtROS) levels, and inhibits the activation of NLRP3 inflammasomes and caspase-1. In the MPTP-induced PD mouse model, SFN improved motor performance, increased the survival rate of tyrosine hydroxylase (TH)-positive dopaminergic neurons in the substantia nigra, and restored dopamine metabolism in the striatum, normalizing the DOPAC/DA and 5-HIAA/5-HT ratios. Electron microscopy revealed that SFN facilitated the clearance of damaged mitochondria through autophagosomes and blocked mtROS-mediated NLRP3 inflammasome activation. In the MPP + -induced BV-2 microglial cell model, SFN upregulated CBS expression, enhanced H₂S synthesis, increased the LC3-II/I ratio, and inhibited p62 degradation, thereby promoting the recovery of mitochondrial membrane potential and reducing ROS release. These effects were still observed under Nrf2 silencing conditions, indicating that SFN's neuroprotective effects are mediated through the CBS–H₂S axis independently of Nrf2 signaling. Collectively, these findings indicate that SFN reshapes the CBS–H₂S signaling axis, activates mitochondrial autophagy, and suppresses inflammation, offering novel insights into multi-target therapeutic approaches for PD and underscoring the essential role of H₂S in neuroprotection.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2026
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IF 5.4
Bin Yao,Huiyun Luo,Mengdie Qu,Xingyan Liu,Lirong Mo,Panyin Wang,Li Wen,Youzhou He,Yuyu Fang,Min Fu,Bowen Lei
Preparation of upconversion efficient In-TCPP(Zn) via an " internally-empowered " Strategy • Synergistic enhancement of light absorption and charge carrier migration via the upconversion effect and IEF • The IZT has highly efficient photocatalytic production capacity of H 2 . In recent years, most current studies on upconversion photocatalysts mainly focus on improving light harvesting, while their deeper role in regulating charge carrier dynamics remains insufficiently explored. Herein, high-photosensitivity Zn-porphyrin (ZnTCPP) was used as a ligand to synthesize In-TCPP(Zn) (IZT-X%, X represents the content of doped metal salts) doped with upconversion elements (Tm 3+ ). Among the series of synthesized materials, IZT-3% exhibited the most outstanding photocatalytic performance (1775 μmol·g −1 ·h −1 ), which is 8.07 times that of pristine In-TCPP(Zn). In-situ X-ray photoelectron spectroscopy ( in-situ XPS), time-resolved photoluminescence (TRPL), upconversion photoluminescence (UCPL) and DFT analyses reveal that the incorporation of Tm 3+ improves the charge distribution and enhances the built-in electric field (IEF) in In-TCPP(Zn), thereby accelerating charge carrier migration. Simultaneously, Tm 3+ can upconvert low-energy near-infrared (NIR) light to visible light that can be absorbed by In-TCPP(Zn), thereby significantly enhancing its photocatalytic performance. This study highlights the key role of upconversion doping in modulating charge carrier dynamics and provides new insights into the synergistic effect of upconversion and the IEF for improving photocatalytic hydrogen evolution efficiency, thereby broadening the utilization of near-infrared light. Download: Download high-res image (205KB) Download: Download full-size image
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IMMUNOBIOLOGY
·
2023
·
IF 2.8
Zepeng Chen,Qinglong Gu,Ruichao Chen
Ulcerative colitis (UC) is a chronic inflammatory disease affecting the colon that can be influenced by microRNAs (miRNAs). This study aims to investigate the impact of miR-146a-5p on lipopolysaccharide (LPS)-induced Caco-2/HT-29 cell autophagy and NLRP3 inflammasome activation and the underlying mechanism, with the aim of identifying potential therapeutic targets. We used LPS to establish Caco-2/HT-29 cell models and measured cell viability by CCK-8. The levels of miR-146a-5p, RNF8, markers of NLRP3 inflammasome activation and autophagy, proteins involved in the Notch1/mTORC1 pathway, and inflammatory factors were assessed by RT-qPCR, Western blot, and ELISA. Intestinal epithelial barrier function was evaluated by measuring transepithelial electrical resistance. Autophagic flux was measured using tandem fluorescent-labeled LC3. miR-146a-5p was highly-expressed in LPS-induced Caco-2/HT-29 cells, and autophagy flux was blocked at the autolysosomal stage after LPS induction. Inhibition of miR-146a-5p suppressed NLRP3 inflammasome activation, reduced intestinal epithelial barrier damage, and facilitated autophagy inhibition in LPS-induced Caco-2/HT-29 cells. The autophagy inhibitor NH4Cl partially nullified the inhibitory effects of miR-146a-5p inhibition on NLRP3 inflammation activation. miR-146a-5p targeted RNF8, and silencing RNF8 partly abrogated the action of miR-146a-5p inhibition on promoting autophagy and inhibiting NLRP3 inflammasome activation. miR-146a-5p inhibition suppressed the Notch1/mTORC1 pathway activation by upregulating RNF8. Inhibition of the Notch1/mTORC1 pathway partially nullified the function of silencing RNF8 on inhibiting autophagy and bolstering NLRP3 inflammasome activation. In conclusion, miR-146a-5p inhibition may be a potential therapeutic approach for UC, as it facilitates autophagy of LPS-stimulated Caco-2/HT-29 cells, inhibits NLRP3 inflammasome activation, and reduces intestinal epithelial barrier damage by upregulating RNF8 and suppressing the Notch1/mTORC1 pathway.
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Reaction Kinetics Mechanisms and Catalysis
·
2023
·Vol. 136
·期 4
·2021-2037
·
IF 1.8
Wang Zhongze,Shi Haonan,Shu Sihao,Zhang Xing,Feng Shangzhen,Chen Jixiang
A series of Ni x In y -BTC metal organic frameworks (x/y denotes the Ni/In atomic ratio) were prepared through the solvothermal method, and then carbonized at 400–700 °C in a N 2 flow followed by the H 2 reduction at 550 °C to prepare carbon-coated Ni–In IMCs catalysts. It has been found that the H 2 reduction is favorable for the formation of Ni–In IMCs and the Ni–In IMCs phases in Ni x In y @C are determined by the Ni/In atomic ratio. Interestingly, a thin layer of carbon (~ 2 nm) coats the Ni–In IMC particles. NiIn 3 C 0.5 IMC forms in Ni 2 In 1 @C, and its crystallite size increases with carbonization temperature. In addition, raising the carbonization temperature promotes the degree of graphitization. In in-situ aqueous phase selective hydrogenation of methyl palmitate to hexadecanol using methanol as hydrogen donor, all the NiIn 3 C 0.5 IMC, NiIn IMC and Ni 2 In 3 IMC dominatingly give hexadecanol, however, Ni 2 In 3 IMC is hydrothermally unstable. Therein, Ni 2 In 1 @C carbonized at 600 °C (i.e., Ni 2 In 1 @C-600/550) exhibits the best performance with the hexadecanol yield of 90.3%, and the hexadecanol yield maintains ~ 90% during 5 times recycling. Such high activity and stability are ascribed to stable NiIn 3 C 0.5 IMC phase, thin carbon layer and suitable degree of graphitization in Ni 2 In 1 @C-600/550.
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JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Hongmei Deng,Meiling Liu,Zhaodong Liu,Minhua Su,Yue Gao,Danxing Yang,Yuxuan Liu,Liang Ma,Zhiqiang Yu,Tangfu Xiao,Jun Luo
Speciation of thallium (Tl) controls its fate and biogeochemical behaviors. Thus, a sensitive and accurately approach for Tl monitoring is of great demand due to its ultra-low concentration and sensitivity to redox change. In this study, diffusive gradients in thin-films technique (DGT) assembled with novel titanium peroxide (TP) binding gels (TP-DGT) was developed for in situ measurement of dissolved Tl(I) and Tl(III) in waters and sediments. Laboratory test showed a linear mass accumulation of Tl(I) and Tl(III) on the TP binding gels with the deployment time from 4 to 72 h. A fascinating performance of this novel DGT was achieved in a pH range of 4–9, ionic strength range of 0.1–200 mmol L −1 , and humic acid concentration of 0–30 mg L −1 with a low detection limit of 0.3 ng L −1 for Tl(I) and 0.6 ng L −1 for Tl(III). The TP binding gels own excellent stability (1–365 d) and high capacity (73.5 μg Tl disc −1 ) which are suitable for long-term monitoring. Field application in a river indicated that TP-DGT could work effectively, comparable to the grab sampling in waters. For the first time, the TP-DGT coupled with oxygen optode was successfully applied to map 2D distribution patterns of Tl and oxygen simultaneously in the rhizosphere of M. verticillatum L. This study confirms TP-DGT is a promising tool for routine monitoring of Tl in waters and for investigating biogeochemical processes of Tl in sediments.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2023
·
IF 3.4
Yutao Lou,Mengting Cheng,Qin Cao,Kening Li,Hui Qin,Meihua Bao,Yuan Zhang,Sisi Lin,Yiwen Zhang
Mirabegron and vibegron, both newly identified beta-3 adrenergic agonists, have significantly improved the quality of life for patients suffering from overactive bladder. In order to comprehensively assess the plasma exposure levels of these agents, the development of a rapid and highly sensitive bioanalytical method becomes imperative. The primary objective of this study was to establish a robust high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the concurrent quantification of mirabegron and vibegron in human plasma. The analytes were extracted from a 100 μL plasma sample through protein precipitation, employing 300 μL of methanol. Subsequently, samples underwent separation and quantification using a Waters XBridge C 18 column (2.1 × 100 mm, 3.5 µm), with a mobile phase consisting of 0.1% formic acid in water and 0.1% formic acid in acetonitrile. The mass analysis was conducted using positive electrospray ionization (ESI+) operated in a multiple reaction monitoring (MRM) mode. The proposed method was meticulously validated in accordance with the guidelines set forth by the U.S. Food and Drug Administration (FDA) for bioanalytical method validation. The regression equations demonstrated exceptional linearity for both mirabegron (r² ≥ 0.994) and vibegron (r² ≥ 0.996) across the concentration range of 0.5 – 200 ng/mL. Furthermore, the assay exhibited accuracy (inter-day relative error ≤ 6.90%) and precision (inter-day coefficient of variation ≤ 8.88%). The average recoveries of the analytes were found to range from 81.94% to 102.02%, with mean matrix effects falling within the range of 89.77% to 110.58%. As a result, this method was deemed highly suitable for the precise determination of the concentrations of both mirabegron and vibegron in the context of therapeutic drug monitoring and bioequivalence studies.
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Journal of Materials Chemistry C
·
2021
·Vol. 9
·期 27
·8537-8544
·
IF 7.393
Zhongyuan Guan,Haihang Ye,Peiwen Lv,Lijin Wang,Jing Zhang,Bin Zou,Aiwei Tang
Multinary Cu-based chalcogenide nanocrystals (NCs) are excellent candidates for optoelectronic applications, but their growth mechanism and its impacts on the NCs’ optical properties remain controversial. Herein, we employed a simple colloidal chemistry method for the growth of multinary Cu-based NCs including ternary Cu–In–S NCs, quaternary Cu–In–Se–S NCs, and five-component Cu–In–Zn–Se–S NCs, to explore their growth mechanism and investigate their optical properties. It is found that the ternary Cu–In–S NCs contain a large number of copper vacancies, which affect the major acceptor level for the exciton recombination. With the high reaction activity of Se, the quaternary Cu–In–Se–S NCs form at low reaction temperature and have a red-shift of the photoluminescence (PL) maximum due to the recombination from the conduction band to the acceptor level. The formation process of five-component Cu–In–Zn–Se–S NCs highly depends on the Zn amount, which shows a competitive relationship between the NCs’ growth and diffusion of Zn, leading to the red- and blue-shift of PL maximum, respectively. Our study provides a clear map for the growth of multinary Cu-based NCs and shows their impacts on optical properties.
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