Food Bioscience
·
2021
·
IF 4.24
Jiao Yuan,Yuvaraj Ravikumar,Guoyan Zhang,Junhua Yun,Yufei Zhang,Hossain M. Zabed,Xianghui Qi
l -arabinose isomerases (L-AIS) are enzymes that mediate the conversion of l -arabinose into l -ribulose and D-galactose into D-tagatose. The potential of L-AI from Lactobacillus parabuchneri CICC 6004 (LPAI) in D-galactose biotransformation using E. coli whole-cells was studied. The parameters investigated in this study were 1) the optimum pH, temperature, and cofactor specificity for maximum enzyme activity ; 2) the D-tagatose biosynthesis from D-galactose using whole-cells containing LPAI. The results showed that with D-galactose, the purified LPAI showed the highest activity, 15.52 U/mg at pH 6.7, 45 °C, and necessitates 3 mM Ca 2+ as its cofactor. The K m , V max and K cat /K m values of LPAI for D-galactose were 5.4 mM, 35.5 U/mg and 2.8 mM −1 min −1 , respectively, while the respective values for l -arabinose were found to be 4.3 mM, 63.1 U/mg and 5.9 mM −1 min −1 .The applicability of LPAI for D-tagatose synthesis was shown by using whole-cell biocatalyst approach. Under the optimum conditions, during one-stage biotransformation of D-galactose by E. coli whole cells, 39.0 g/L of D-tagatose was produced from 140 g/L of D-galactose after 50 h, which corresponded to the yield and productivity of 0.28 g/g and 0.78 g/L/h, respectively. Further addition of new cells into the reaction mixture in the second stage produced 54.0 g/L of D-tagatose after 100 h. The D-tagatose total yield and productivity were 0.39 g/g and 0.54 g/L/h, respectively. The results attained in this study showed LPAI as a potential candidate for the industrial production of D-tagatose.
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Small
·
2021
·Vol. 18
·期 9
·2104120
·
IF 13.281
Ziyu Wu,Rui Wu,Xiaoyun Li,Xingwen Wang,Xueting Tang,Kaiyuan Tan,Mimi Wan,Chun Mao,Xingquan Xu,Huiming Jiang,Jiawei Li,Min Zhou,Dongquan Shi
Most of the current non-pharmacological treatment strategies for atherosclerosis (AS) suffer from poor penetration into the plaque and only aim at a certain factor in its formation process, resulting in limited therapeutic effect. Herein, a kind of nanomotor with dual-mode propulsion is constructed, which is sensitive to higher reactive oxygen species (ROS) at the AS site and near-infrared (NIR) laser by the covalent binding and self-assembly of β-cyclodextrin (β-CD) and L-arginine (LA) with immobilization of Au nanoparticles. NIR laser irradiation can be used as a driving force and to ablate inflammatory macrophages through the photothermal effect. The nitric oxide (NO) released by the nanomotors can be used as another driving force and a therapeutic agent to promote endothelial repair in the plaque site. LA can eliminate ROS in the inflammatory site, and β-CD can promote the removal of cholesterol from foam cells. In particular, the two driving modes of nanomotors synergistically promote their aggregation and penetration in the plaque. This kind of nanomotor can regulate the microenvironment of AS in multiple ways, including combination therapy for endothelial repair, lipid clearance, and reducing ROS, which is expected to become a potential non-pharmacological strategy in the treatment of AS.
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高影响因子
Redox Biology
·
2019
·
IF 7.793
Shen-Fei Wang,Xin Liu,Mo-Yu Ding,Shuangcheng Ma,Jing Zhao,Ying Wang,Shaoping Li
Reducing agents are crucial for the management of maladaptive inflammation-induced macrophage death and hematopoietic toxicity of chemotherapy. 2- O - β - d -glucopyranosyl- l -ascorbic acid (AA-2 β G), a unique AA (or vitamin C) derivative identified in Lycium barbarum , exhibited enhanced free radical scavenging activity compared with AA and its synthetic derivative AA-2 α G. AA-2 β G protected hydrogen peroxide-induced cell death in murine macrophage RAW264.7 cells. Treatment with AA-2 β G eliminated oxidative stress and the ratio of cellular glutathione to glutathione disulfide more effectively than AA and AA-2 α G. AA-2 β G also significantly reduced the fluorescent intensity of DCFH-DA triggered by chemotherapeutic agent camptotehcin-11 but not fluorouracil. AA, AA-2 α G, and AA-2 β G significantly decreased Keap-1expression, and increased the expression levels of nuclear factor E2-related factor 2 (Nrf2) and heme oxygenase-1. All compounds triggered the nuclear translocation of Nrf2, while the ability of AA-2 β G to enhance the Nrf2-DNA binding affinity was approximately two fold as those of AA and AA-2 α G. Sodium ascorbate cotransporters (SVCT) inhibitors, sulfinpyrazone, phloretin, and 3- O -methyglucose, potently abrogated the free radical scavenging activities of AA, AA-2 α G, and AA-2 β G. The cellular uptake efficacy of AA-2 α G and AA-2 β G was less than 10% of AA, while the inhibition of SVCT with sulfinpyrazone considerably diminished the uptake efficacy of these compounds. AA-2 α G and AA-2 β G are more stable in the Fenton reagents than AA. In summary, AA-2 β G from L. barbarum with excellent free radical scavenging activity is a promising natural AA derivative for further pharmacological evaluation.
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高影响因子
Journal of Translational Medicine
·
2025
·Vol. 23
·期 1
·1385
·
IF 7.5
Gong Jiaxin,Wei Feng,Wu Tianyu,Zhang Ningzhi,Zhang Yongqiao,Cui Zhaoqiang,Ge Junbo
Background Obesity-related cardiac injury is a serious global condition associated with excessive fat intake, which is strongly linked to chronic low-degrade inflammation. Pyroptosis, a pro-inflammatory form of cell death, has been implicated in obesity recently. L-Fucose (Fuc), a sulfated polysaccharide with anti-inflammatory and antioxidant properties, remains unexplored in the area of pyroptosis and obesity-related cardiac injury. Methods Six-week-old wide type C57BL/6 mice were subjected to high-fat diet (HFD) feeding for 20 weeks to induce obesity with or without Fuc gavaging, followed by assessment of metabolic related indicators. Echocardiography, histopathological analysis and enzymatic assays were used to assess cardiac function. The level of pro-inflammatory cytokines was detected by ELISA, qRT-PCR and CD68 staining. Pyroptosis-related protein including NLRP3, GSDMD, pro-caspase-1, cleaved-caspase-1, IL-1β, and ASC were evaluated by western blot. Oxidative stress was detected by ROS level. Cardiac mitochondrial structure was assessed by electron microscopy and mitotracker staining. Mitochondrial function was measured by mitochondrial membrane potential (MMP) and oxygen consumption rate (OCR) assay. Molecular docking and molecular dynamics (MD) simulations were conducted to look for the potential target of Fuc. Cellular thermal shift assay (CETSA) and western blot were used to investigate the interaction between Fuc and its target. Palmitic acid (PA) induced lipotoxicity model in AC16 cells was used to further confirm the protective effects of Fuc and its downstream signaling pathway against inflammation, pyroptosis and mitochondrial injury in vitro. Results In vivo, Fuc treatment alleviated obesity-related weight gain, hyperlipidemia, elevated systolic blood pressure and cardiac diastolic dysfunction. It also reduced the expression of pro-inflammatory cytokines and pyroptosis-related proteins, the level of oxidative stress, and mitochondrial abnormalities in the myocardium. Moreover, the vitro lipotoxicity model further confirmed the protective effect of Fuc. The molecular docking analysis identified TLR4 as the key target of Fuc. Fuc reduced the level of TLR4 and its downstream proteins MyD88 and p-NF-κB, while activating TLR4/MyD88/NF-κB pathway by TLR4 agonist Neoseptin3 reversed the protective function of Fuc in vitro. Conclusions These findings demonstrated that Fuc mitigated cardiac inflammation, pyroptosis and mitochondrial injury by inhibiting the TLR4/MyD88/NF-κB pathway, highlighting its potential as a therapeutic agent for obesity-associated cardiac injury.
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PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·
IF 12.6
Lizhen Lin,Xinghong Zhou,Baizhao Peng,Jing Ma,Zhuping Zheng,Cuiping Jiang,Zhuoen He,Shuyuan Li,Wen Fang,Chuanghai Wu,Zaihao Jiang,Yao Chen,Lina Zhong,Hiu Yee Kwan,Chunyan Shen,Shenhai Gong,Xiaoshan Zhao
Ulcerative colitis (UC) is characterised by chronic intestinal inflammation and its global prevalence is increasing. Although a variety of drugs have been approved for the clinical treatment of UC, their application is often limited by unsatisfactory long-term effects, side effects, and high treatment costs. Therefore, there is an urgent need for the development of effective drugs with fewer side effects. In this study, we found that the extracellular vesicles extracted and purified from rhizomes of Kaempferia galanga L. (KGEVs) exhibit promising therapeutic effects in treating UC disease. With an average diameter of 133.8 nm, KGEVs are rich in functional components, including lipids, proteins, and pharmacologically and immunologically active molecules. In vivo experiments revealed that KGEVs accumulated in the colorectal region 6 h after oral administration, demonstrating targeted enrichment at the site of enteritis. Moreover, we found that KGEVs effectively alleviate DSS-induced colitis in mice, as indicated by reductions in body weight loss, DAI score, spleen index and colon length shortening. Mechanistically, KGEVs may alleviate colitis by repairing the intestinal barrier, inhibiting oxidative stress and colonic inflammation regulating gut microbiota and inhibiting the polarisation of macrophages into pro-inflammatory M1 macrophages during the inflammatory response, indicating significant anti-inflammatory effects. These results suggest the potential of KGEVs as a promising, cost-effective, and efficient oral therapeutic agents for UC treatment.
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高影响因子
Journal of Macromolecular Science Part A-Pure and Applied Chemistry
·
2025
·
IF 2.4
Shenghua Xu,Weihong Guo
A novel magnetic nanocomposite was successfully synthesized by grafting Poly(L-glutamic acid) (PLGA) onto Fe3O4 nanoparticles, achieved through the carboxylation of a Fe3O4-graft-poly(γ-benzyl-L-glutamate) (Fe3O4-g-PBLG) precursor with trimethyliodosilane. The chemical structure of the resulting Fe3O4-graft-Poly(L-glutamic acid) (Fe3O4-g-PLGA) was unequivocally confirmed by 1H NMR and FT-IR spectroscopy. This composite exhibited superparamagnetic behavior (saturation magnetization: 6.73 emu/g) and enhanced hydrophilicity (contact angle: 36.6°). Porous scaffolds were fabricated via a well-established solution casting and particulate leaching technique using NaCl as a porogen. By precisely controlling the porogen amount (10 g and 15 g per gram of composite), scaffolds with tailorable macroporous architecture were obtained, featuring pore sizes of 250 ± 30.98 μm and 450 ± 39.72 µm, and interconnected porosities of 90.2 ± 2.95% and 96.9 ± 3.67%, respectively. A comprehensive evaluation of the scaffolds’ physical properties revealed high water retention capacities (1179 ± 73.26% and 1051 ± 69.35%), tunable compressive moduli (0.35 ± 0.05 MPa and 0.21 ± 0.02 MPa), and viscoelastic storage moduli ranging from 5500 to 6500 Pa. In vitro degradation studies in PBS over 20 wk demonstrated controllable biodegradation, with mass losses of 58.22 ± 2.36% and 68.69 ± 2.77%, correlating with the scaffold porosity. Finally, preliminary cytocompatibility assessment using adipose-derived stem cells indicated favorable cell adhesion on the scaffold surface, as observed by SEM and LCSM. The tailorable porosity, robust mechanical properties, and superparamagnetic nature of the Fe3O4-g-PLGA scaffolds render them a versatile functional material with broad potential in the field of polymeric biomaterials. However, its comprehensive biological evaluation, including direct cytotoxicity tests and in vivo performance assessment, is an important direction for future research.
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高影响因子
Environmental Pollutants and Bioavailability
·
2025
·
IF 3.2
Hongchuan Li,Yusef Kianpoor Kalkhajeh,Peidong Cong,Jianjun Liu,Hongxiang Hu,Fuxiang Li
This study investigated the efficacy of GLDA (C9H9NNa4O8) for the remediation of moderately Cd-contaminated soil (total Cd, 0.47 mg/kg). A 50-day pot experiment was carried out with T. patula (Tagetes patula L.) subjected to five GLDA treatments (0, 0.25, 0.5, 1 and 2 mmol/kg). The GLDA doses were administered in two equal parts, with a 15-day time interval between them. Our results indicated that GLDA treatments significantly increased the Cd content in the aboveground and root of T. patula, as well as soil pH and soil DTPA-Cd content compared with the control treatment (CK) (P < 0.05). Under 2 mmol/kg, an average reduction of 9.85% occurred in soil Cd content compared with the CK. Furthermore, GLDA enhanced the transformation of residual and organic-bound Cd into exchangeable and iron-manganese oxide forms. At concentrations of 0 and 0.25 mmol/kg, GLDA increased the richness of soil bacterial communities, whereas inhibition occurred at higher application rates (1 and 2 mmol/kg). The Chao1 and Shannon indices of 1 and 0.5 mmol/kg significantly increased by 29.9% and 7.98%, respectively, compared with the CK (P < 0.05). Proteobacteria, Acidobacteria, Chloroflexia, and Myxococcota were the dominant bacterial phyla in Cd remediation by T. patula induced by GLDA, reflecting their high tolerance to Cd contamination. Altogether, this study elucidates how GLDA enhances Cd remediation through its impacts on soil Cd mobility/fractionation and soil microbial composition, presenting a novel and sustainable/ecofriendly phytoremediation strategy for moderately Cd-contaminated soils.
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ChemCatChem
·
2021
·Vol. 14
·期 3
·e202101489
·
IF 5.686
Lingling Peng,Wansen Tan,Yuting Lu,Ayan Yao,Dayuan Zheng,Le Li,Jingran Xiao,Lijun Li,Qingbiao Li,Shu-feng Zhou,Guowu Zhan
Graphical Immobilized Rha on MOFs : Herein, we explored five typical metal-organic frameworks (MOFs) as porous carriers to immobilize Rha, and the obtained Rha@Ce-BTC showed the highest enzymatic activity. Also, Rha@Ce-BTC manifested enhanced enzyme-substrate affinity, great reusability/storage stability, and catalytic efficiency compared to free Rha. Significantly, the as-prepared Rha@Ce-BTC might be assembled as a membrane reactor for efficient biocatalysis reactions. The α-L-Rhamnosidase (Rha) is a useful glycoside hydrolase for selectively hydrolyzing the terminal L-rhamnose residues in flavonoids, being vital to food and pharmaceutical industries. However, Rha suffers from low recyclability and poor stability in harsh environments. Herein, we explored five typical metal-organic frameworks (MOFs) as porous carriers to immobilize Rha, and the activities of the resultant Rha@MOF composites were compared with the free enzyme. The locations of the enzyme in MOFs were proved by a series of characterization techniques. It was found that Rha@Ce-BTC (with enzyme immobilization efficiency of 23 % and enzyme loading content of 8.8 %) showed the highest enzymatic activity. The immobilized Rha@Ce-BTC showed 80 % residual activity after five consecutive cycles, suggesting a limited leaching effect. Also, Rha@Ce-BTC manifested markedly enhanced enzyme-substrate affinity and catalytic efficiency compared to free Rha, as supported by Michaelis-Menten kinetic studies. Accordingly, the Ce-BTC would be an appealing carrier for enzyme immobilization, and the as-designed enzyme/Ce-BTC composites are promising candidates for industrial use with remarkably high activity, recyclability, and storage stability.
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高影响因子
ACS Infectious Diseases
·
2021
·
IF 5.084
Maochao Zheng,Huanchang Lin,Wancong Zhang,Shijie Tang,Daojun Liu,Jianfeng Cai
Multifunctional antimicrobial peptides that combine the intrinsic microbicidal property of cationic polypeptide chains and additional antibacterial strategy hold promising applications for the treatment of infections caused by antibiotic-resistant bacteria, especially “superbugs”. In the present study, star-shaped copolymers ZnPc-g-PLO with a zinc phthalocyanine (ZnPc) core and four poly(l-ornithine) (PLO) arms were designed, synthesized, and evaluated as dual-functional antimicrobial agents, that is, intrinsic membrane damage and photothermal ablation capacity. In an aqueous solution, amphiphilic ZnPc-g-PLO molecules self-assemble into nanosized polymeric micelles with an aggregated ZnPc core and star-shaped PLO periphery, where the ZnPc core exhibits appreciable aggregation-induced photothermal conversion efficiency. In the absence of laser irradiation, ZnPc-g-PLO micelles display potent and broad-spectrum antibacterial activities via physical bacterial membrane disruption as a result of the high cationic charge density of the star-shaped PLO. Upon laser irradiation, significant improvement in bactericidal potency was realized due to the efficacious photothermal sterilization from the ZnPc core. Notably, ZnPc-g-PLO micelles did not induce drug-resistance upon subinhibitory passages. In summary, dual-functional ZnPc-g-PLO copolymers can serve as promising antibacterial agents for the treatment of infectious diseases caused by antibiotic-resistant bacteria.
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高影响因子
Separations
·
2021
·Vol. 8
·期 10
·186
·
IF 2.777
Zili Guo,Shuting Xiong,Yuanyuan Xie,Xianrui Liang
Ellagic acid is a phenolic compound that exhibits both antimutagenic and anticarcinogenic activity in a wide range of assays in vitro and in vivo. It occurs naturally in some foods such as raspberries, strawberries, grapes, and black currants. In this study, a valid and reliable method based on mechanochemical-assisted extraction (MCAE) and macroporous adsorption resin was developed to extract and prepare ellagic acid fromPhyllanthus urinariaL. (PUL). The MCAE parameters, acidolysis, and macroporous adsorption resin conditions were investigated. The key MCAE parameters were optimized as follows: the milling time was 5 min, the ball mill speed was 100 rpm, and the ball mill filling rate was 20.9%. Sulfuric acid with a concentration of 0.552 mol/L was applied for the acidolysis with the optimized acidolysis time of 30 min and acidolysis temperature of 40 °C. Additionally, the XDA-8D macroporous resin was chosen for the purification work. Both the static and dynamic adsorption tests were carried out. Under the optimized conditions, the yield of ellagic acid was 10.2 mg/g, and the content was over 97%. This research provided a rapid and efficient method for the preparation of ellagic acid from the cheaply and easily obtained PUL. Meanwhile, it is relatively low-cost work that can provide a technical basis for the comprehensive utilization of PUL.Keywords:Phyllanthus urinariaL.;ellagic acid;mechanochemical-assisted extraction;macroporous adsorption resin
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高影响因子
Acta Biomaterialia
·
2016
·
IF 6.008
Weijun Xu,Junmin Qian,Yaping Zhang,Aili Suo,Ning Cui,Jinlei Wang,Yu Yao,Hejing Wang
To mimic the structure of breast tumor microenvironment, novel double-network poly(N ɛ -acryloyl l -lysine)/hyaluronic acid (pLysAAm/HA) hydrogels were fabricated by a two-step photo-polymerization process for in vitro three-dimensional (3D) cell culture. The morphology, mechanical properties , swelling and degradation behaviors of pLysAAm/HA hydrogels were investigated. The growth behavior and function of MCF-7 cells cultured on the hydrogels and standard 2D culture plates were compared. The results showed that pLysAAm/HA hydrogels had a highly porous microstructure with a double network and that their mechanical properties, swelling ratio and degradation rate depended on the degree of methacrylation of HA. The results of in vitro studies revealed that the pLysAAm/HA hydrogels could support MCF-7 cell adhesion , promote cell proliferation , and induce the diversification of cell morphologies and overexpression of VEGF, IL-8 and bFGF . The MCF-7 cells cultured on 3D hydrogels showed significantly increased migration and invasion abilities as compared to 2D-cultured cells. Preliminary in vivo results confirmed that the 3D culture of MCF-7 cells resulted in greater tumorigenesis than their 2D culture. These results indicate that the pLysAAm/HA hydrogels can provide a 3D microenvironment for MCF-7 cells that is more representative of the in vivo breast cancer. Statement of Significance Traditional 2D cell cultures cannot ideally represent their in vivo physiological conditions. In this work, we reported a method for preparing double-network poly(N ɛ -acryloyl l -lysine)/hyaluronic acid hydrogel, and demonstrated its suitability for use in mimicing breast tumor microenvironment. Results showed the prepared hydrogels had controllable mechanical properties, swelling ratio and degradation rate. The MCF-7 cells cultured in hydrogels expressed much higher levels of pro-angiogenic growth factors and displayed significantly enhanced migration and invasion abilities. The tumorigenic capability of MCF-7 cells pre-cultured in 3D hydrogels was enhanced significantly. Therefore, the novel hydrogel may provide a more physiologically relevant 3D in vitro model for breast cancer research. To our knowledge, this is the first report assessing a HA-based double-network hydrogel used as a tumor model.
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高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
·
2024
·Vol. 42
·期 5
·570-578
·
IF 4.1
Sun Xiao-Fei,Zhang Xu,Song Shu-Ping,Yao Ya-Qun,Zhang Yan,Wang Cheng-Liang,Sun Jing-Jiang,Wang Qing-Fu
L -glutamic acid (LA) is a bio-based, non-toxic, environmentally friendly material derived from biomass. The present study reports the application of Passerini three-component polymerization (P-3CP) for the straightforward preparation of LA-based light-responsive polyesters (PLTDs) under mild conditions. PLTDs with molar masses up to 8500 g/mol and high yields exceeding 90% are obtained. The chemical structures and light-responsive self-immolative behavior of PLTDs are comprehensively characterized by employing ultraviolet-visible (UV-Vis) spectroscopy, size exclusion chromatography (SEC), nuclear magnetic resonance (NMR) spectroscopy, and liquid chromatography mass spectrometry (LC-MS). Meanwhile, monodisperse PLTD-based doxorubicin-loaded nanoparticles (PLTD-DOX-NP) (size=193 nm, PDI=0.018) are formulated by nanoprecipitation method. Upon light-induced depolymerization, the PLTD-DOX-NP undergoes rapid decomposition, resulting in a burst release of 80% cargo within 13 s. Furthermore, according to biological toxicity tests, the PLTD-NP possesses adequate biosafety, both before and after irradiation. Overall, the incorporation of P-3CP with biorenewable LA-based monomer adheres to the principles of green chemistry, significantly simplifying the synthetic pathway of light-responsive polymers.
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