FOOD CHEMISTRY
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2017
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IF 4.529
Ruyan Hou,Mengmeng Tong,Wanjun Gao,Lei Wang,Tianxi Yang,Lili He
To investigate the degradation and penetration behaviors of the organophosphate insecticide dimethoate applied on spinach leaves, in situ SERS and LC-MS methods were used to detect dimethoate residue on&in spinach leaves picked on different days after treatment (DAT). The SERS and LC-MS methods determined that the dimethoate degradation rate followed first-order kinetics, with a half-life of 3.56 or 4.13 days, depending on the respective method. The correlation coefficient of quantification value of these two methods was 0.9562. With the SERS method, we detected most of the penetrated dimethoate in the depth of 60–110 μm. With the LC-MS method, we detected dimethoate up to 0.17 ng in leaves and that the dimethoate on:in ratio of spinach leaves ranged from 562.25 on 0 DAT to 5.23 on 14 DAT. The combination of these two methods facilitated a better understanding of the behavior and biological fate of pesticides in a complex biological system.
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NANO
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2017
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IF 1.025
Xiuhua Li,Jian Lin,Junhong Zhao
A novel composite nanostructure which is made up of TiO 2 three dimensionally ordered macroporous (3DOM) nanostructure and TiO 2 nanorods (NRs) has been successfully synthesized through a combination of colloidal crystal template technology and hydrothermal method, then we achieved its combination with Ag nanoparticles (NPs) via a silver mirror reaction. We studied the SERS (Surface-Enhanced Raman Scattering) performance of the obtained structure, the results show that our samples are very sensitive substrates when being used to detect dye R6G molecules, with a low detection concentration of 10 − 1 1 M. This proves that it is a promising material in the area of analyzing and molecule-level detecting as a kind of novel and low-cost SERS substrate.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2024
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IF 4.3
Zhu-Rui Zhang,Ying Chen,Zhi-Wei Wang,Chang-Ying Hu,Yuling Hu,Xiaowen Xu
Polylactic acid (PLA) straws hold eco-friendly potential; however, residual diisocyanates used to enhance the mechanical strength can generate carcinogenic primary aromatic amines (PAAs), posing health risks. Herein, we present a rapid, comprehensive strategy to detecting PAAs in 18 brands of food-grade PLA straws and assessing their migration into diverse food simulants. Surface-enhanced Raman spectroscopy was conducted to rapidly screen straws for PAAs. Subsequently, qualitative determination of migrating PAAs into various food simulants (4 % acetic acid, 10 % ethanol, 50 % ethanol) occurred at 70 °C for 2 h using liquid chromatography-mass spectrometry. Three PAAs including 4,4′-methylenedianiline, 2,4′-methylenedianiline, and 2,4-diaminotoluene were detected in all straws. Specifically, 2,4-diaminotoluene in 50 % ethanol exceeded specific migration limit of 2 μg/kg, raising safety concerns. Notably, PAAs migration to 10 % and 50 % ethanol surpassed that to 4 % acetic acid within a short 2-hour period. Moreover, PLA straws underwent varying degrees of shape changes before and after migration. Straws with poly(butylene succinate) resisted deformation compared to those without, indicating enhanced heat resistance, while poly(butyleneadipate-co-terephthalate) improved hydrolysis resistance. Importantly, swelling study unveiled swelling effect wasn’t the primary factor contributing to the increased PAAs migration in ethanol food simulant, as there was no significant disparity in swelling degrees across different food simulants. FT-IR and DSC analysis revealed higher PAAs content in 50 % ethanol were due to highly concentrated polar ethanol disrupting hydrogen bonds and van der Waal forces holding PLA molecules together. Overall, minimizing contact between PLA straws and alcoholic foods is crucial to avoid potential safety risks posed by PAAs.
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ACS Applied Materials & Interfaces
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2024
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IF 8.3
Jiapu Li,Yuan Feng,Li Liang,Feng Liao,Wanxia Huang,Kuanguo Li,Guanglei Cui,Zewen Zuo
Flexible surface-enhanced Raman scattering (SERS) substrates are very promising to meet the needs for real-time and on-field detection in practical applications. However, high-performance flexible SERS substrates often suffer from complexity and high cost in fabrication, limiting their widespread applications. Herein, we developed a facile method to fabricate a flexible multicavity SERS substrate composed of a silver nanoparticle (AgNP)-decorated aluminum hydrous oxide nanoflake array (NFA) grown on a polydimethylsiloxane (PDMS) membrane. Strong plasmon couplings promoted by multiple nanocavities afford high-density hotspots within such a flexible AgNPs@NFA/PDMS film, boosting high SERS sensitivity with an enhancement factor (EF) of ∼1.54 × 109, and a limit of detection (LOD) of ∼7.4 × 10–13 M for rhodamine 6G (R6G) molecules. Furthermore, benefiting from the high sensitivity, high mechanical stability, and transparency of this substrate, in situ SERS detections of trace thiram and crystal violet (CV) molecules on the surface of cherry tomatoes and fish have been realized, with LODs much lower than the maximum allowable limit in food, demonstrating the great potential of such a flexible substrate in food safety monitoring. More importantly, the preparation processes are very simple and environmentally friendly, and the techniques involved are completely compatible with well-established silicon device technologies. Therefore, large-area fabrication with low cost can be readily realized, enabling the extensive applications of SERS sensors in daily life.
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Polymers
·
2024
·Vol. 17
·期 1
·81
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IF 4.7
Xinyi Liu,Hongji Li,Dandan Wang,Jian Lu,Yilin Wu,Wei Sun
Chrysoidin (CG) can be ingested into the human body through the skin and cause chronic toxicity, so the detection of CG levels in the environment is crucial. In this study, we synthesize F-Ag@ZIF-8/PVC molecular-imprinted membranes (FZAP-MIM) by an innovative combination of SERS detection, membrane separation, and a molecular-imprinted technique in order to perform the analysis of CG in water. The plasmonic MOF material as a SERS substrate helps to enrich the target and realize the spatial overlap of the target with the nanoparticle tip “hotspot”. To avoid the poor reproducibility of Raman signals caused by the random arrangement of the powder substrate, polyvinyl chloride (PVC) is used to provide support and protection for the powder substrate. PVC has excellent dirt immunity and chemical stability, enabling the substrate to maintain Raman performance under complex and extreme detection conditions. FAZP-MIM has outstanding sensitivity and selectivity and can quickly and accurately capture targets even in the presence of similar structural interferences. The method showed superior recoveries in spiked recovery tests of real water samples and is expected to be practically applied to the trace detection of organic dye molecules in the environment.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2024
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IF 5.4
Dechan Lu,Bohan Zhang,Zhikun Shangguan,Yudong Lu,Jingbo Chen,Zufang Huang
Exosomes, extracellular vesicles released by cells, hold potential as diagnostic markers for the early detection of lung cancer. Despite their clinical promise, current technologies lack rapid and effective means to discriminate between exosomes derived from adenocarcinoma in situ (AIS) and early-stage invasive adenocarcinoma (IAC). This challenge arises from the intrinsic structural heterogeneity of exosomes, necessitating the development of advanced methodologies for precise differentiation. Here, we demonstrate a novel approach for plasma exosome detection utilizing multi-receptor surface-enhanced Raman spectroscopy (SERS) technology to differentiate between AIS and early-stage IAC. To accomplish this, we synthesized a stable and uniform two-dimensional SERS substrate (BC/Au NPs film) by fabricating gold nanoparticles onto bacterial cellulose. We then enhanced its capabilities by introducing multi-receptor SERS functionality via modifying the substrate with both low-specificity and physicochemical-selective molecules. Furthermore, by strategically combining all capturer-exosome SERS spectra, comprehensive “combined-SERS spectra” are reconstructed to enhance spectral variations of the exosome. Combining these features with partial least squares regression-discriminant analysis (PLS-DA) modeling significantly improved discriminatory accuracy, achieving 90% sensitivity and 95% specificity in distinguishing AIS from early-stage IAC. Our developed SERS sensor provides an effective method for early detection of lung cancer, thereby paving a new way for innovative advancements in diagnosing lung cancer.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2025
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IF 9.7
Baichuan Zhao,Sijie Liao,Jun Cao,Aiguo Shen
The combination of in-situ SERS monitoring and photocatalysis could track reaction process, and deepen understanding of underlying mechanism of photocatalysis. The study of photocatalytic behavior driven by organic semiconductor is not as clear as plasmonic and inorganic semiconductor photocatalysis. Herein, an organic semiconductor hybrids reactor was constructed by decorating gold nanoparticles (Au NPs). The hybrids achieved in-situ SERS monitoring of photocatalytic degradation of rhodamine 6G with considerable sensitivity and uniformity. This has been attributed to the photocatalytic performance of organic semiconductor and the SERS effect of uniformly modified Au NPs. In particular, the hybrids monitored the photocatalytic reduction of 4-nitrobenzenethiol on its surface by in-situ SERS tracking the formation of intermediate product 4, 4′-dimercaptoazobenzene and end product 4-aminothiophenol. This work introduced organic semiconductor into photocatalysis and in-situ SERS monitoring field for enhancing understanding of the photocatalytic mechanism on organic surface, and developing the application potential of SERS technology.
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SENSORS AND ACTUATORS B-CHEMICAL
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2017
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IF 5.401
Ya-Ning Wang,Ying Zhang,Wen-Shu Zhang,Zhang-Run Xu
A novel surface-enhanced Raman scattering substrate was developed through in situ growing gold nanoparticles inside the mesoporous graphitic carbon nitride with a large surface area. The mesoporous structure afforded support for loading more gold nanoparticles, defined the nanoparticle diameter and ensured uniform distribution of the nanoparticles. Crystal violet used as a Raman probe was detected by SERS based on the hybrids with an enhancement factor of 5.0 × 10 5 , and the relative standard deviation of the peak intensity (at 1174 cm −1 and 1618 cm −1 ) were 6.2% and 6.1% (n = 30) for solid samples, and 5.1% and 5.0% (n = 11) for liquid samples. The high SERS activity was attributed to abundant hot spots formed by high-density gold nanoparticles and chemical enhancement from mpg-C 3 N 4 , and the good reproducibility was due to controlled size and uniform distribution of the gold nanoparticles. An antineoplastic drug, 6-thioguanine was detected with a wide linear range from 0.60 μM to 48 μM and a low detection limit of 0.24 μM. The mesoporous g-C 3 N 4 embedded with gold nanoparticles significantly improved the SERS sensitivity and reproducibility for 6-thioguanine detection, and it holds promising potential in noninvasive and ultrasensitive drug monitoring.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 2
·1-11
·
IF 5.3
Li Kaiwei,Jiang Han,Wang Liying,Wang Rui,Zhang Xuewei,Yang Libin,Jiang Xin,Song Wei,Zhao Bing
Semiconductor materials have become a competitive candidate for surface-enhanced Raman scattering (SERS) substrate. However, powdered semiconductors are difficult to execute a fast in situ detection for trace analytes. Here, we developed a new flexible semiconductor SERS substrate by in situ densely growing anatase TiO 2 nanoparticles on the surface of cotton fabric through a filtration-hydrothermal method, in which TiO 2 exhibits excellent controllability in size and distribution by regulating the ratio of water to alcohol in synthesis and the number of filtration-hydrothermal repetitive cycle. Cotton fabric/TiO 2 (Cot/TiO 2 ) substrate exhibits a high SERS activity and excellent spectral repeatability. The developed substrate has an ultra-high stability that can withstand long-term preservation; it can even resist the corrosions of strong acid and alkali, as well as high temperature up to 100 °C and low temperature down to − 20 °C. The flexible substrate can be used to carry out a rapid in situ detection for quinolone antibiotic (enrofloxacin and enoxacin) residues on the fish body surface by using a simple swabbing method, with high quantitative detection potential (up to an order of magnitude of 10 −7 M), and even for the simultaneous detection of both drug residues. The flexible substrate also exhibits an excellent recyclability up to 6 recycles in the actual SERS detection. Graphical Abstract
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Analytical Methods
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2024
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IF 2.7
Xiaoxia Lu,Wenlong Lu,Dong Hua
In the study, a novel surface-enhanced Raman scattering (SERS)-lateral flow assay (LFA) tray for the real-time detection of pyroptosis-associated miR-155-5p in breast cancer cells was established and validated. The SERS probe modified with monoclonal antibodies and functionalized HP1@5-FAM was first synthesized. When miR-155-5p was present, HP1@5-FAM on the SERS probe specifically recognized target miRNAs and hybridized with them, resulting in HP2 on the T line only capturing some SERS probes that were not bound to miR-155-5p. The T line appeared as a light orange band or there was no color change, and the corresponding Raman detection result showed a weak or insignificant Raman signal. The SERS probe showed high selectivity, satisfactory stability, and excellent reproducibility, and the limit of detection (LOD) for miR-155-5p was 7.26 aM. Finally, the proposed SERS-LFA tray was applied to detect miR-155-5p in MBA-MD-468 cells that underwent varying degrees of pyroptosis, and the detection results of SERS were consistent with those of the conventional real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay. The study demonstrated that the SERS-LFA tray was a convenient and ultrasensitive method for miR-155-5p real-time detection, which could provide more detailed information for pyroptosis and be of potential value in guiding the treatment of breast cancer.
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Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Xinyi Liu,Huiyan Wei,Meiqi Ju,Chunying Pang,Dandan Wang,Yuming Liu,Shuhua Zhang,Hongji Li
Tryptophan is a potential biomarker for a variety of diseases, so rapid and accurate detection of tryptophan levels in the human body is essential. In this work, surface-enhanced Raman scattering (SERS) detection was combined with acid-catalyzed silicon-based molecularly imprinted polymers to synthesize Cu 2 O@Ag-MIPs (CA-MIPs) for selective detection of L-tryptophan(L-Trp). Compared with traditional detection methods, SERS detection has the advantages of being sensitive, fast and nondestructive. Here, Ag nanoparticles are uniformly loaded on {100} surface-dominated Cu 2 O sub-microcubes to excite strong localized surface plasmon resonance, which exhibits good SERS performance. Considering the complexity of practical detection, an acid-catalyzed method was adopted to load silicon-based imprinted polymers with high biocompatibility on the Cu 2 O@Ag surface, which improved the drawbacks of the common silicon-based imprinting method, such as loose structure and long reaction time. The thickness of the imprinted layer can be controlled to ensure selectivity while ensuring superior SERS sensitivity. The prepared CA-MIPs were shown good linearity with L-Trp concentration in the range of 10 −3 –10 −10 mol L −1 , and the sensor also exhibited excellent selectivity for the determination of L-Trp in the presence of other interfering amino acids. In addition, the method was shown good recoveries in spiked recovery tests of simulated urine samples and was expected to be practically applied to the detection of clinical disease markers.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Xinya Zhang,Qiong Ning,Xiao Meng,Mengping Zhang,Wenwen Chen,Ming Shang,Xiaomei Li,Cuijuan Wang
Rapid detection of antidepressant intoxication is of significant clinical importance for accurate diagnosis and timely resuscitation. Herein, we developed a point-of-care diagnostics (POCD) approach that integrates surface-enhanced Raman spectroscopy (SERS) and efficient adsorption. This point-of-use sensor was constructed by modifying silver octahedral hollow cages (AgOHC) onto a porous melamine sponge (MS) in a 96-well plate. The etching conditions were optimized to create a high density of nanogaps on the AgOHC surface, thereby generating sufficient hotspots. Clear trends were observed between the SERS strength and the concentrations of duloxetine and escitalopram using this sensor. This system exhibits detection limits for duloxetine and escitalopram in gastric juice, urine, and serum as low as 1.04–45.23 ng/mL, sufficiently sensitive for clinical diagnosis of antidepressant poisoning. Furthermore, the feasibility was evaluated by applying it to serum samples from patients. The results obtained using this method are in accordance with those obtained using liquid chromatography–mass spectrometry. Compared with conventional methods, this approach represents an effective method with satisfactory recovery and rapid detection time (∼1 min), rendering it suitable for POCD. This cost-effective and user-friendly SERS-based POCD approach provides a novel alternative for rapid detection in non-laboratory settings and holds considerable promise for clinical applications.
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