RSC Advances
·
2015
·Vol. 5
·期 29
·22343-22349
·
IF 3.84
Zhao Chen,Xiaochi Wang,Hua Li,Chao Li,Qinghua Lu,Guang Yang,Jiangang Long,Lingjie Meng
A facile but effective bottom-up method for the mass preparation of N-doped carbon dots (N–C-dots) was developed by the direct heating of a solid mixture of folic acid (FA) and sodium citrate (SC) for several minutes at 300 °C. The nitrogen content of the resulting N–C-dots could be easily and precisely tuned from 0% to 14% by adjusting the SC to FA mass ratio. Uniform N–C-dots with honeycomb-like ordered structures were obtained, with sizes ranging from 1 to 3 nm in diameter. The N-doping content affected not only the emission quantum yield but also the emission wavelength. The N–C-dots emitted strong blue-green fluorescence based on the excitation wavelength and N-doping content. Because of their excellent water solubility, low toxicity, powerful fluorescence, and high resistance to photobleaching, the N–C-dots can enter various cells and serve as ideal candidates for multicolour cell imaging.
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RSC Advances
·
2016
·Vol. 6
·期 95
·92739-92747
·
IF 3.289
Li Zhang,Wei Wan,Qiang Wang,Wen-Chao Lu,Bei-Hua Hou,Ping Chen
Catalysts for the oxygen reduction reaction (ORR) are crucial in metal–air batteries, fuel cells and other electrochemical devices. Developing heteroatom-doped carbon catalysts with high performance and stability in alkaline media for practical application of metal–air batteries or in acidic media for practical application of proton exchange membrane fuel cells is still difficult and challenging. Here, we report the N-, Fe-doped carbon sphere/oriented carbon nanofiber nanocomposite (NFe-CS/CNF). N-, Fe-doped carbon spheres (NFe-CS) were derived from p-phenylenediamine and oriented N-, Fe-doped carbon nanofibers (NFe-CNF) were derived from o-phenylenediamine. 3.89 at% of N and 0.38 at% of Fe were doped in a typical product, which has high surface area. The typical product exhibits a high ORR activity and stability in acidic media and reveals superior stability and tolerance to methanol than Pt/C. In alkaline media, the typical product shows superior activity to Pt/C. The performance of a zinc–air full cell under real battery operation conditions indicates that the typical product is a competitive alternative to commercial PtC. The unique structure of NFe-CS/CNF could create strong interactions between the nanospheres and oriented nanofibers, promote charger transfer at the interface and improve the conductivity. This work provides a new fundamental understanding and design strategy for excellent carbon-based ORR catalysts.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2016
·
IF 2.857
Lingmei Li,Chengjian Wang,Shan Qiang,Jixiang Zhao,Shuang Song,Wanjun Jin,Bo Wang,Ying Zhang,Linjuan Huang,Zhongfu Wang
Glycosylation of many proteins has been revealed to be closely related with food allergies, and screening and structural analysis of related glycoproteins and glycoallergens are essential for studies in this field. Herein, we describe detailed N-glycoform analysis of all glycoprotein fractions of soybean protein isolate (SPI) separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) to disclose structural features of the glycan moieties of more soybean glycoproteins. SPI was fractionated by SDS-PAGE, and the generated protein bands were recovered and subjected to in-gel N-glycan release and labeling using a one-pot method newly developed by our group, followed by detailed analysis by electrospray ionization mass spectrometry (ESI-MS) and online hydrophilic interaction liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HILIC-ESI-MS/MS). As a result, we found seven bands mainly containing oligomannose-type glycans; two mainly contain core α1,3-fucosylated glycans, and six have no glycans. This study is the first report that discovers core α1,3-fucosylated N-glycans in bands 1, 2, and 6 and discloses bands 3, 4, 5, and 7 as glycoproteins and their N-glycoforms. Therefore, it can expand our knowledge about soybean protein glycosylation and provide significant structural reference for research of soybean allergens.
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PLoS One
·
2017
·Vol. 12
·期 1
·e0170013
·
IF 2.806
Sheng Liu,Wenjie Gao,Yao Wang,Zhenyu He,Xiaojun Feng,Bi-Feng Liu,Xin Liu
Monitoring glycosylation of the mAbs have been emphasized and routinely characterized in biopharmaceutical industries because the carbohydrate components are closely related to the safety, efficacy, and consistency of the antibodies. In this study, the comprehensive glycan profiling of a biosimilar candidate of cetuximab was successfully characterized using Normal phase high-performance liquid chromatography (NP-HPLC) in combination with Matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). The presence of minor N-linked glycans containing sialic acid lactone residues (NeuAcLac) was observed in the biosimilar for the first time, which could influence the quantitative analysis of sialylated glycans and interfere with quantification of neutral glycans when it was analyzed by high performance liquid chromatography fluorescence (HPLC-FL). To overcome this issue, mild alkali treatment was used to hydrolyze lactone of the sialic acid to their neutral formation, which had no impact on the analysis of other glycans before and after the treatment. As a result, the mild alkali treatment might be helpful to obtain quantitative glycan profiling of the mAbs drugs with enhanced accuracy and robustness.
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JOURNAL OF ALLOYS AND COMPOUNDS
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2016
·
IF 3.014
Ting Lü,Shuang Zhang,Dongming Qi,Dong Zhang,Hongting Zhao
A class of thermosensitive and reusable magnetic nanoparticles (MNPs) was developed for removing emulsified oil droplets from aqueous media. Magnetic iron oxide (MIO) nanoparticles were prepared with a coprecipitation method, followed by coating with silica layer and further modification with γ-methacryloxypropyl triisopropoxidesilane (MPS). Poly( N -isopropylacrylamide) (PNIPAM) molecular chains were then anchored onto the surfaces of MNPs via a “grafting through” reaction. The efficiency of synthetic MNPs for treating emulsified oily wastewater was evaluated as a function of dosage, pH, temperature and reusability. PNIPAM-grafted MNPs exhibited high demulsification efficiency at lower temperature, while decreased dramatically when the temperature exceeded the lower critical solution temperature (LCST) of PNIPAM (∼33 °C); moreover, MNPs could be facilely regenerated by rinsing with hot water and could be reused up to 7 cycles. The results indicated that PNIPAM-grafted MNPs could potentially be a new promising and environmentally friendly approach for effectively treating emulsified oil in wastewater.
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Journal of Materials Chemistry B
·
2017
·Vol. 5
·期 22
·4052-4059
·
IF 4.543
Heping Wang,Fenglong Jiao,Fangyuan Gao,Junjie Huang,Yan Zhao,Yehua Shen,Yangjun Zhang,Xiaohong Qian
Protein glycosylations play important roles in various biological processes and in the disease progression of organisms. The development of specific enrichment materials and strategies before mass spectrometric analysis was a prerequisite to glycoproteomic analysis due to the difficulty caused by substoichiometric levels of glycoproteins. In this work, novel magnetic covalent organic frameworks (denoted as Fe3O4@TpPa-1) were first developed using only a two-step solvothermal reaction and then applied in the hydrophilic enrichment of glycopeptides. Sea urchin-type composites with super-paramagnetic properties were constructed by in situ growth of TpPa-1 covalent organic frameworks on the surface of magnetic nanoparticles. A total of 37 and 22 glycopeptides could be easily detected from IgG and HRP digests, respectively, by hydrophilic enrichment with the newly developed materials. An ultralow detection limit (28 fmol), satisfactory selectivity and high recovery could be achieved using Fe3O4@TpPa-1. The material's excellent enrichment performance was also demonstrated using glycopeptide analysis in real complex samples. Within three independent replicates, 228 glycopeptides corresponding to 114 glycoproteins could be detected from human serum digests, which is better than the performance obtained by commercial HILIC materials. The results suggest that covalent organic frameworks show potential for application in glycoproteomic studies.
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CARBOHYDRATE POLYMERS
·
2017
·
IF 4.811
Zehong Chen,Xinwen Peng,Xiaoting Zhang,Shuangshuang Jing,Linxin Zhong,Runcang Sun
Producing hierarchical porous N-doped carbon from renewable biomass is an essential and sustainable way for future electrochemical energy storage. Herein we cost-efficiently synthesized N-doped porous carbon from renewable cellulose by using urea as a low-cost N source, without any activation process. The as-prepared N-doped porous carbon (N-doped PC) had a hierarchical porous structure with abundant macropores, mesopores and micropores. The doping N resulted in more disordered structure, and the doping N content in N-doped PC could be easily tunable (0.68–7.64%). The doping N functionalities could significantly improve the supercapacitance of porous carbon, and even a little amount of doping N (e.g. 0.68%) could remarkably improve the supercapacitance. The as-prepared N-doped PC with a specific surface area of 471.7 m 2 g −1 exhibited a high specific capacitance of 193 F g −1 and a better rate capability, as well as an outstanding cycling stability with a capacitance retention of 107% after 5000 cycles. Moreover, the N-doped porous carbon had a high energy density of 17.1 W h kg −1 at a power density of 400 W kg −1 .
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ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Yingfang Yao,Yong You,Gaixia Zhang,Jianguo Liu,Haoran Sun,Zhigang Zou,Shuhui Sun
Tuna is one of the most rapid and distant swimmers. Its unique gill structure with the porous lamellae promotes fast oxygen exchange that guarantees tuna’s high metabolic and athletic demands. Inspired by this specific structure, we designed and fabricated microporous graphene nanoplatelets (GNPs)-based Fe/N/C electrocatalysts for oxygen reduction reaction (ORR). Careful control of GNP structure leads to the increment of microporosity, which influences the O2 adsorption positively and desorption oppositely, resulting in enhanced O2 diffusion, while experiencing reduced ORR kinetics. Working in the cathode of proton-exchange membrane fuel cells, the GNP catalysts require a compromise between adsorption/desorption for effective O2 exchange, and as a result, appropriate microporosity is needed. In this work, the highest power density, 521 mW·cm–2, at zero back pressure is achieved.
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JOURNAL OF MOLECULAR LIQUIDS
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2016
·
IF 2.74
Jinshou Wang,Jing Jin,Yanbin Tan,Zhen Shi,Shenghu Zhang
We have constructed the pseudoternary phase diagram of surfynol465/ n -butanol/ethyl acetate/water with mass ratio of surfynol465 to n -butanol being 1.0. In the O/W microemulsion region, we have measured the rate of a first-order hydrolysis reaction of tert-butyl bromide near and far away from the critical point. In the noncritical region, the rate constants of the reaction obey respective Arrhenius equation. In the critical region, the rate constants did not deviate from Arrhenius equation, and no critical effect was apparent, which agreed with the Griffiths–Wheeler rule. This work extended the studies of critical effect on the chemical reaction to more complicated O/W microemulsion media and added to the current interest in microemulsion system functioning as media for chemical reactions.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Sisi Fan,Minggang Zhao,Longjiang Ding,Ye Ma,Jingjing Liang,Xingtao Wang,Yawen Song,Shougang Chen
Eggshell membranes were used as biotemplate to fabricate a 3D hierarchical porous Co 3 O 4 /ZnO p-n junction film. Multiple catalysis and accelerated electron transfer were achieved on the Co 3 O 4 /ZnO-GOx electrode by introducing the p-n junction interface into enzyme loading matrix. A stable and well-defined oxidation peak of glucose was obtained with a largely decreased detection potential of 0.25 V. Furthermore, an enhanced sensitivity (116.64 μA·mM −1 ·cm −2 ) was also achieved comparing with the simple biotemplate-synthesized Co 3 O 4 and ZnO film. The results demonstrated it is a novel strategy to improve the biosensing performance by introducing p-n junction interface into enzyme loading matrix.
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ADVANCED FUNCTIONAL MATERIALS
·
2016
·Vol. 26
·期 9
·1400-1410
·
IF 11.382
Tao Ji,Qian Liu,Rujia Zou,Yangang Sun,Kaibing Xu,Liwen Sang,Meiyong Liao,Yasuo Koide,Li Yu,Junqing Hu
A multicolor photodetector based on the heterojunction of n-Si(111)/TiO 2 nanorod arrays responding to both ultraviolet (UV) and visible light is developed by utilizing interface engineering. The photodetector is fabricated via a consecutive process including chemical etching, magnetron sputtering, hydrothermal growth, and assembling. Under a small reverse bias (from 0 to ≈−2 V), only the photogenerated electrons in TiO 2 are possible to tunnel through the low barrier of Δ E C , and the device only responses to UV light; as the reverse bias increases, the photogenerated holes in Si also begin to tunnel through the high barrier of Δ E V . As a result, the device is demonstrated to have the capacity to detect both UV and visible lights, which is useful in the fields of rapid detection and multicolor imaging. It has been also observed that the crystal orientation of Si affects the characteristics of bias-controlled spectral response of the n-Si/TiO 2 heterojunctions.
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SENSORS
·
2016
·Vol. 16
·期 11
·1874
·
IF 2.033
Qi Xiao,Shuangyan Lu,Chusheng Huang,Wei Su,Shan Huang
Based on N-doped carbon dots/β-cyclodextrin nanocomposites modified glassy carbon electrodes (N-CDs/β-CD/GCE), an effective electrochemical sensor for enantioselective recognition of tryptophan (Trp) enantiomers was developed by differential pulse voltammograms (DPVs). Fluorescent N-CDs were synthesized through a hydrothermal method and characterized by spectroscopic approaches. The N-CDs/β-CD nanocomposites were efficiently electrodeposited on the surface of GCE through C–N bond formation between N-CDs and electrode. The obtained N-CDs/β-CD/GCE was characterized by multispectroscopic and electrochemical methods. Such N-CDs/β-CD/GCE generated a significantly lower Ip and more negative Ep in the presence ofl-Trp in DPVs, which was used for the enantioselective recognition of Trp enantiomers. The N-CDs/β-CD nanocomposites showed different binding constants for tryptophan enantiomers, and they further selectively bonded withl-Trp to form inclusion complexes. This N-CDs/β-CD/GCE combined advantages of N-CDs with strong C–N binding ability and β-CD with specific recognition of Trp enantiomers to fabricate a novel sensing platform for enantioselective recognition of Trp enantiomers. This strategy provided the possibility of using a nanostructured sensor to discriminate the chiral molecules in bio-electroanalytical applications.Keywords:N-doped carbon dots;β-cyclodextrin;tryptophan enantiomers;enantioselective recognition;differential pulse voltammetry
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