MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 6
·1-10
·
IF 6.232
Liu Xingyu,Li Xiangqian,Dong Pei,Wu Zhiming,Gao Jinwei,Wang Qianming
A low-molecular-weight molecule (4-(2-(3-(dicyanomethyl)-5,5-dimethylcyclohex-1-en-1-yl)vinyl)phenyl-benzoate, DDPB) has been developed. The organic framework possesses very weak fluorescence . The feasibility of the signal transduction has been performed via fluorometric titrations in solution. DDPB gives rise to responses to carboxylesterase 2 (CES2) based on “off-on” responses. The red emission at 670 nm has been derived from the enzyme-induced hydrolysis of ester linkages, thus suppressing the intramolecular charge transfer (ICT) effect and thereby generating the fluorescent segment. The optical excitation window for this probe is extended to the visible light range ( λ ex = 516 nm), and it will induce less harmful influence on biological substances. The detection limit for the measurement of CES2 concentration is as low as 2.33 mU/mL. The conventional studies concerning the activation process are generally performed within only a single liveing cell system. In this study, it is the first time that expression of carboxylesterase 2 in five kinds of cell lines (HeLa > C1498 > active T cell > Jurkat > unactive T cell) has been clarified by flow cytometry, Western blotting, and confocal microscopy analysis. The elucidation of CES2 and its variability in a variety of cells will open new ways for drug metabolism and disease prevention.
We reported a new “substrate-mediated light-on” strategy based on an ester bond cleavage reaction. Most of prepared nanomaterials and organic fluorophores possessed short wavelength emissions in the blue or green region which will not be difficult for cellular imaging. In this study, a novel functional molecule (DDPB) was considered as the substrate for CES2 and the optical “off-on” response was realized. DDPB was cell permeable and possessed very low cytotoxicity. Moreover, the identification of CES2 and their subtle changes in five different cells afforded the sequence for carboxylesterase-2 as Hela > C1498 > Active T cell > Jurkat > Unactive T cell. Inhibition studies showed that the hydrolysis of DDPB was effectively suppressed by bis-p-nitrophenyl phosphate and the cellular tracking results firmly supported this point. To our knowledge, the inter-individual variability for the CES2 expressions in five different cell lines has never been reported via the substrate induced optical changes.
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JOURNAL OF FLUORESCENCE
·
2020
·Vol. 30
·期 3
·679-685
·
IF 2.093
Chen Xuanming,Hu Na,Wei Huifeng,Wang Haibo
Chirality plays a very important role in medicine, biochemistry and other fields. Because the enantiomers of chiral drugs often show different pharmacology activity, metabolism, and toxicity, therefore, the recognition of chiral molecules is very important, and has become a hot spot and frontier of modern chemical research. In this paper, a new method for recognizing chiral molecular based on naphthalimide dye(NA)⊂cucurbit[5]uril(CB[7]) assembly is developed. NA as guest can be combined with the host CB[7] to form a 1:1 NA⊂CB[7] assembly. Furthermore, this assembly was used as a fluorescent probe to recognize D/L-phenylalanine and D/L-phenylalaninol by fluorescence titration. When D-phenylalanine or D-phenylalaninol was added to NA⊂CB[7] assembly, the fluorescent intensity of assembly was partially quenched, but when L-phenylalanine or L-phenylalaninol was added to NA⊂CB[7], the fluorescence intensity of the assembly almost unchanged. Herein, chiral recognition platform based on achiral NA⊂achiral CB[7] was constructed.
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CELLULOSE
·
2020
·Vol. 27
·期 15
·8843-8858
·
IF 4.21
Jiang Mengting,Zhang Yakang,Chen Jingwen,Liang Qian,Xu Song,Yao Chao,Zhou Man,Li Zhongyu
A series of morphology-controlled BiOBr nano/macrostructures was successfully synthesized via a novel biomass-route derived from bacterial cellulose (BC). In the BiOBr/BC system compared with pure BiOBr, the three-dimensional BC scaffolds not only effectively prevented the aggregation of BiOBr units by pore confinement, but also strengthened the controllability of BiOBr size with the help of abundant anchoring sites provided by cross-linked BC fibers. By associating the results of characterizations and the photodegradation of RhB dye, the relationship between morphology and photocatalytic activity of BiOBr/BC was investigated. In addition, a possible mechanism for the enhanced photocatalytic activity of the optimized BiOBr/BC has been discussed. This biomass-derived route provides a platform for further design of bismuth-based composite photocatalysts with potential advantages of being anti-aggregation, highly-dispersed, easily recovered and having enhanced catalytic performance.
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METALLURGICAL AND MATERIALS TRANSACTIONS A-PHYSICAL METALLURGY AND MATERIALS SCIENCE
·
2020
·Vol. 51
·期 10
·5328-5338
·
IF 2.05
Zou Yongshun,Gu Huazhi,Huang Ao,Fu Lvping
Sintered magnesia refractories combined by in situ intergranular CaZrO 3 phases were synthesized using natural MgO-containing natural minerals with CaO and SiO 2 impurities and nano-sized ZrO 2 additive. A homogenous distribution of intergranular CaZrO 3 , independent from the intergranular CaO-MgO-SiO 2 phases, was formed in situ within the sintered magnesia aggregates by introducing 0.75 wt pct nano-sized ZrO 2 into the magnesite. The formation mechanism of the in situ intergranular CaZrO 3 phases was determined. The nano-sized ZrO 2 was introduced and uniformly distributed at grain boundaries of the magnesia due to the micron-nano-sized particles composite system and wetting grinding process. Then the CaO in impurities were prior to SiO 2 to react with the ZrO 2 for generating CaZrO 3 at the grain boundaries by increasing sintering temperature. Nevertheless, the nano-sized ZrO 2 particles were encapsulated in the MgO crystallites with similar particle size decomposed from brucite and prevented from reacting with CaO impurities in magnesite. The mixing homogeneity of magnesite particles and ZrO 2 particles and the direct contact between ZrO 2 and CaO impurities in magnesite has a crucial effect on the formation of intergranular CaZrO 3 phases. Furthermore, the intergranular CaZrO 3 phases could enhance the bonding of magnesia grains and have great potential for improving the service performance of magnesia.
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Journal of Advanced Ceramics
·
2020
·Vol. 9
·期 6
·693-702
·
IF 2.889
Xu Jie,Lu Qiling,Lin Jinfeng,Lin Cong,Zheng Xinghua,Lin Tengfei,Wu Xiao
Er 3+ -doped Ba 0.85 Ca 0.15 Ti 0.9 Zr 0.1 O 3 ( x Er-BCTZ, x = 0, 0.005, 0.01, 0.015) multifunctional thick films were prepared by the tape-casting method, using sol-gel-derived nano-sized powders as the matrix material. The surface morphologies, photoluminescence, and electrical properties were investigated. Dense microstructures with pure perovskite structure were obtained in the thick films. By doping an appropriate amount of Er 3− , the samples exhibit superior up-conversion photoluminescence performance and simultaneously enhanced electrical performances. In addition, relatively higher texture fractions (with the largest value of 83.5%) were realized through introducing plate-like BaTiO 3 templates to make the thick film grow by the [001] c orientation. And the ferro-/piezoelectric properties of the thick films were further improved, showing potential in the applications of micro-optoelectronic devices.
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Nature Communications
·
2020
·Vol. 11
·期 1
·1-13
·
IF 12.121
Jia Chao,Li Lei,Liu Ying,Fang Ben,Ding He,Song Jianan,Liu Yibo,Xiang Kejia,Lin Sen,Li Ziwei,Si Wenjie,Li Bo,Sheng Xing,Wang Dongze,Wei Xiaoding,Wu Hui
Advanced ceramic sponge materials with temperature-invariant high compressibility are urgently needed as thermal insulators, energy absorbers, catalyst carriers, and high temperature air filters. However, the application of ceramic sponge materials is severely limited due to their complex preparation process. Here, we present a facile method for large-scale fabrication of highly compressible, temperature resistant SiO 2 -Al 2 O 3 composite ceramic sponges by blow spinning and subsequent calcination. We successfully produce anisotropic lamellar ceramic sponges with numerous stacked microfiber layers and density as low as 10 mg cm −3 . The anisotropic lamellar ceramic sponges exhibit high compression fatigue resistance, strain-independent zero Poisson’s ratio, robust fire resistance, temperature-invariant compression resilience from −196 to 1000 °C, and excellent thermal insulation with a thermal conductivity as low as 0.034 W m −1 K −1 . In addition, the lamellar structure also endows the ceramic sponges with excellent sound absorption properties, representing a promising alternative to existing thermal insulation and acoustic absorption materials.
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Communications Biology
·
2020
·Vol. 3
·期 1
·1-12
·
IF 4.165
Zhou Lulu,Chen Lv,Hu Xiaochun,Lu Yonglin,Liu Wenjie,Sun Yanting,Yao Tianming,Dong Chunyan,Shi Shuo
Despite its great potential in cancer therapy, phototherapy, including photothermal therapy (PTT) and photodynamic therapy (PDT), often cause metastasis of tumors. Immunotherapy has revolutionized the cancer treatment owing to the capability of activating immune system to eliminate tumors. However, the integration of phototherapy and immunotherapy in a single nanoagent for cancer therapy is still a challenging task. Here, we fabricated (Cu 9 S 5 @mSiO 2 -PpIX@MnO 2 @CpG (CSPM@CpG)) as a synergistic therapeutic model for phototherapy enhanced immunotherapy. The intracellular uptake of cytosine-phosphate-guanine (CpG) promoted the infiltration of cytotoxic T lymphocytes (CTLs) in tumor tissue, further stimulating the production of interferon gamma (IFN-γ) and remarkably elevating the immune response level. Excellent anti-tumor effects have been achieved by synergistic PTT/PDT/immunotherapy. The metastasis of tumors was effectively inhibited by the immune response of CpG. Thus, our proposed work provides a strategy to combine phototherapy with immunotherapy to enhance the therapeutic efficiency and further inhibit metastasis of tumors.
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Nano Research
·
2020
·Vol. 13
·期 8
·2106-2117
·
IF 8.183
Li Zhongtao,Tang Jiaoqing,Wu Hongfeng,Ling Zhixin,Chen Siyu,Zhou Yong,Guo Bo,Yang Xiao,Zhu Xiangdong,Wang Lin,Tu Chongqi,Zhang Xingdong
Melanoma is a highly malignant skin tumor which is prone to recurrence and metastasis. Hydroxyapatite nanoparticles (nHAPs) were reported to possess a suppressive effect on proliferation of various tumor cells in vitro. This study aimed to assess in vitro and in vivo anti-tumor ability and biosafety of the nHAPs used in the treatment of melanoma. Three types of nHAPs with different morphology and crystallinity were synthesized. In vitro cell viability and proliferation studies demonstrated that all three types of nHAPs can inhibit viability and proliferation of A375 and SK-MEL-28 melanoma cells in a concentration-dependent manner. In addition, the rod-shape nHAPs with a crystallinity of 45.60% had the most prominent suppressive effect on the two melanoma cells tested. An important positive regulator of G1/S phase transition in cell cycle, Cyclin D1 protein, was reduced by nHAPs treatment in vivo. We further discovered that the migration ability of the nHAPs treated melanoma cells was greatly decreased. RNA sequencing result revealed that melanoma metastasis related genes were down-regulated by nHAPs, including MMP2, MMP14, ITGA9, ITGB3, ITGB4 and S100B. High concentration of nHAPs treatment in melanoma-bearing nude mice showed a strong inhibitory effect on tumor size and weight. Most importantly, hemolysis, electrolyte disturbance or inflammation response was not discovered in the experimental animals from nHAPs treated groups. We proved that the nHAPs synthesized in the current study has a selective effect to suppress melanoma tumor proliferation and was safe with regard to normal cells and tissue.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2020
·Vol. 27
·期 24
·30778-30788
·
IF 3.056
Wang Guanghui,Luo Qiuyan,Dai Jialing,Deng Nansheng
In this work, the β-cyclodextrin modified magnetic graphene oxide (β-CD/MGO) composite was fabricated by the in situ co-precipitation method and characterized by Fourier transform-infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), vibrating sample magnetometer (VSM), and particle size analysis. The adsorption behavior of dichromate ions on the β-CD/MGO was investigated, and the mechanism of adsorption was also studied using FT-IR and XPS. The results from SEM and TEM showed that the graphene oxide (GO) layer became rough, and many fine particles were attached after compounding with ferroferric oxide and β-cyclodextrin. The characterization results of FT-IR and XPS show that that β-cyclodextrin and ferroferric oxide have been perfectly compounded to the graphene oxide layer and β-CD/MGO has a particle size of about 460 nm, a specific surface area of 252.3 m 2 g −1 , and a saturation magnetization of 73.5 emu g −1 . The adsorption amount of dichromate ions on the β-CD/MGO is affected by pH, adsorbent dosage, and adsorption time. Kinetic studies showed that the adsorption process followed a pseudo-second-order kinetic model. Equilibrium data agreed very well with the Langmuir model, the maximum adsorption amount of dichromate ions on the β-CD/MGO was 49.95 mg g −1 . After five successive adsorbent reuses, the reuse rate is still 73%, indicating the excellent potential reusability of β-CD/MGO adsorbent. β-CD/MGO exhibits excellent adsorption performance for dichromate ions. As an environmentally friendly magnetic adsorbent, β-CD/MGO is suitable for the treatment of dichromate-containing wastewater.
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INFLAMMOPHARMACOLOGY
·
2020
·Vol. 28
·期 6
·1705-1716
·
IF 3.238
Zheng Kai,Jia Jia,Yan Shihai,Shen Hong,Zhu Ping,Yu Jiangyi
Immunological tolerance is critical for maintaining gut homeostasis. An imbalance between interleukin-17 (IL-17)-producing T helper 17 (T H 17) cells and regulatory T cells (T reg cells) is involved in ulcerative colitis (UC) pathogenesis. Dendritic cells (DCs) are able to induce T cell differentiation. Paeoniflorin (PF) is a monoterpene glucoside that is commonly used for treatment of autoimmune disease. However, the immunological mechanism of PF involvement in UC treatment is unclear. The present study aimed to explore whether PF can restore the T H 17/T reg balance by modulating DCs. The effects of PF on DCs, T H 17 cells and T reg cells were measured. Furthermore, PF-treated DCs were injected into mice with 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis. PF inhibited MHC-II and CD86 expression on the DC surface ( P < 0.05), decreased interleukin (IL)-12 secretion in vitro and in vivo ( P < 0.05), and restored the T H 17/T reg ratio in the mouse model of colitis ( P < 0.05). PF-treated DCs diminished T H 17 differentiation (4.26% in vitro and 1.64% in vivo) and decreased IL-17 expression ( P < 0.05) while inducing CD4 + CD25 + Foxp3 + T reg differentiation (7.82% in vitro and 6.85% in vivo) and increasing Foxp3 and IL-10 production ( P < 0.05). Additionally, both PF and PF-treated DCs improved colonic histopathology in the mouse model of colitis ( P < 0.05). In conclusion this study suggested that PF can ameliorate TNBS-induced colitis by modulating the DC-mediated T H 17/T reg balance.
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JOURNAL OF POLYMER RESEARCH
·
2020
·Vol. 27
·期 9
·1-9
·
IF 2.426
Luo ChunHui,Zhao Yufei,Sun Xinxin,Luo FaLiang
Fabricating robust and multi-functional hydrogels is of great importance and challenge. In this work, chitosan (CS) and polyvinyl alcohol (PVA) were used to design antiseptic, conductive and robust hydrogels by a two-step method. Chemical structures of gels, the degree of crystallinity, the state of water in hydrogels, as well as their microstructures were characterized via a combination of FT-IR, XRD, DSC and SEM. Segment lengths of cross-linking points were calculated from elastic rubber theory. Their mechanical properties were evaluated on the electronic testing machine. It was shown that the tensile strength and elongation at break of single PVA hydrogel were only 200 kPa and 135%, respectively, due to the heterogenous structure with pore sizes between 1.5 ~ 8.2 μm. By introducing CS into PVA matrix followed with soaking in a saturated NaCl solution, the network became homogeneous with a pore size of 0.5 ~ 1.1 μm. Moreover, free water changed to bond water, and frictions between polymer chains increased because of hydrophobic associations and entanglements of CS segments. As a result, the tensile stress and strain increased to 3800 kPa and 270%, respectively. The gel also exhibited antiseptic property, electrical conductivity and swelling-resistant properties. The strength after reaching swell equilibrium was 3400 kPa, much higher than most gels at swollen states. This gel might find applications in bionic cartilage, sensors, food preservation and wearable devices.
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JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
·
2020
·Vol. 326
·期 1
·185-192
·
IF 1.137
Hu Ke,Liu Zhirong,Xiu Taoyuan,Zhou Limin,Wang Yun
The Cu 3 (BTC) 2 (BTC = 1,3,5-benzenetricarboxylic acid) was synthesized by water/solvent heat method. The Cu 3 (BTC) 2 was characterized by SEM/EDS, Zeta potential and XRD. Batch static adsorption experiments investigated the various factors affecting the adsorption of Th(IV) in aqueous solution such as pH, solid–liquid ratio, contact time, initial concentration and temperature, and the adsorption mechanism is also discussed. The adsorption process was in line with the pseudo-second-order kinetic model and the Langmuir isotherm model. At the same time, compared with other adsorbents. The results showed that Cu 3 (BTC) 2 had good adsorption for Th(IV) with a maximum adsorption of 757.58 mg g −1 .
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