ANALYTICA CHIMICA ACTA
·
2017
·
IF 4.95
Haijiao Zheng,Tenggao Zhu,Xiqian Li,Jiutong Ma,Qiong Jia
A facile online method coupling polymer monolithic microextraction (PMME) with mass spectrometry (MS) was developed for the detection of Immunoglobulin G (IgG) galactosylation glycopeptides . A peanut agglutinin- β -cyclodextrin (PNA- β -CD) functionalized poly(hydroxyethyl methylacrylate-ethyleneglycol dimethacrylate) monolith was designed via a click reaction. Thanking to the specificity of PNA- β -CD for the targets, the material exhibited enhanced enrichment selectivity and extraction efficiency for IgG galactosylation glycopeptides. Under optimal conditions, the developed method gave a linear range of 0.005–5 pmol for IgG glycopeptides with the regression coefficient greater than 0.9990, and the detection limit of IgG galactosylation glycopeptides as low as 0.5 fmol was achieved. The PMME-MS method was applied to IgG galactosylation glycopeptides in real samples including human serum and acute myelogenous leukemia (AML) cell lysate. A series of unique IgG galactosylation glycopeptides were captured by the monolith in the complex samples, indicating satisfactory enrichment ability for IgG galactosylation glycopeptides. The quick and integrated online PMME-MS method exhibited high selectivity for IgG galactosylation, demonstrating its perspectives on the development and broad applications of MS in studying galactosylation proteins regulated biological processes .
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2017
·Vol. 40
·期 7
·1548-1555
·
IF 2.557
Yufeng Zhou,Haijiao Zheng,Jiutong Ma,Qiong Jia
In the present study, a novel poly(acrylamide- co - N -isopropylacrylamide- co -ethylene dimethacrylate) monolithic column modified with saline-modified delaminated layered double hydroxides was developed and exploited as the stationary phase in the polymer monolith microextraction of β-agonists. Transmission electron microscopy, Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy, and thermal gravimetric analysis with derivative thermogravimetric determination were employed to characterize the monoliths. By coupling with high-performance liquid chromatography determinations, extraction parameters affecting the extraction efficiency, including sample volume, flow rate, sample pH, eluent volume, and eluent flow rate were investigated with an orthogonal experiment design, L 16 (4 5 ). Results demonstrated that under the optimal experimental conditions, low limits of detection in the range of 0.025–0.280 ng/mL were obtained with relative standard deviations in the range of 4.3–7.6% (intraday) and 6.7–8.8% (interday). When the developed method was applied to the analysis of β-agonists in pork samples, recovery values were obtained in the range of 80.5−108.6%.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Polymer Chemistry
·
2013
·Vol. 4
·期 10
·3012-3019
·
IF 5.231
Haibo Wang,Fangming Xu,Yin Wang,Xiangsheng Liu,Qiao Jin,Jian Ji
The pH-triggered biodegradable micelles based on poly(β-amino ester)-graft-12-acryloyloxy dodecyl phosphorylcholine (PAE-graft-ADPC) were explored as an efficient acid-triggered anticancer drug delivery system. A pH-responsive biodegradable polymer was synthesized by using hydrophilic 12-acryloyloxy dodecyl phosphorylcholine (ADPC) and poly(β-amino ester) (PAE) via Michael addition reaction. Micelles with phosphorylcholine shells were prepared in aqueous solution and doxorubicin (DOX) was loaded into the core of the micelles with high efficiency (73%). Dynamic light scattering (DLS) and transmission electron microscopy (TEM) demonstrated that the size of the drug-loaded micelles was about 230 nm. DOX-loaded micelles were stable in the physiological environment, whereas they disassembled at pH 6.5 which is similar to the extracellular pH of most tumors. In vitro drug release showed that DOX release from micelles was faster at pH 6.5 than at 7.4. Intracellular uptake demonstrated that DOX was delivered into the cells effectively after the cells were incubated with DOX-loaded micelles at pH 6.5. Moreover, an in vitro cytotoxicity study indicated that the growth of HepG2 cells was inhibited remarkably when the cells were preincubated with DOX-loaded micelles at pH 6.5. The results indicated that the pH-triggered biodegradable micelles could serve as promising drug carriers for cancer therapy.
✓ 已验证
产品关联
DOI
高影响因子
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2013
·Vol. 236
·期 5
·817-826
·
IF 1.436
Zhang Hongzhi,Li Wei,Rui Xin,Sun Xingmin,Dong Mingsheng
A novel food-grade strain Lactobacillus plantarum 70810 producing β-galactosidase with high transgalactosylation activity was isolated from Chinese paocai. The galactooligosaccharides (GOS) were synthesized by using this enzyme with a maximum yield of 44.3 % (w/w) from 400 g/L lactose at 45 °C for 10 h. The β-galactosidase from this strain was purified to homogeneity by ammonium sulfate precipitation, anion exchange chromatography and gel filtration chromatography. It was a heterodimer arrangement of approximately 105 kDa composed of two subunits of 35 and 72 kDa. The optimal pH of the purified β-galactosidase was 8.0 for both o -nitrophenyl-β- d -galactopyranoside ( o NPG) and lactose hydrolysis, and optimal temperature was 60 °C and 55 °C, respectively. Its K
m
and V
max values for o NPG and lactose were 0.89 ± 0.05 mM, 194 ± 3.0 μmoL/min/mg protein, and 9.88 ± 0.16 mM, 15.88 ± 0.21 μmoL/min/mg protein, respectively. This enzyme was slightly inhibited by the hydrolysis products, that is, glucose and galactose. Since the β-galactosidase from L. plantarum 70810 exhibited higher transgalactosylation activity, strong affinity for lactose and low end-product inhibition, it was suggested to be a potential candidate for the synthesis of prebiotic GOS.
✓ 已验证
产品关联
DOI
高影响因子
POWDER TECHNOLOGY
·
2012
·
IF 2.08
Wanyue Shao,Ruinian Hua,Wei Zhang,Yue Tian,Jun Zhao,Liyan Na,Jicheng Yu,Zhengang Sun
Carboxylic acid-functionalized Yb 3 + /Er 3 + co-doped α - and β -NaYF 4 nano-/micro-spheres were prepared through a simple poly(acrylic acid) (PAA)-assisted hydrothermal process. Bovine serum albumin (BSA) protein was conjugated with Yb 3 + /Er 3 + co-doped α -( β -)NaYF 4 upconversion phosphors via free carboxylic acid groups on the surface of phosphors. The final products were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), infrared (IR) spectrophotometer, ultraviolet (UV) spectrophotometer and photoluminescence spectroscopy (PL). XRD results showed that pure α -NaYF 4 :Yb 3 + , Er 3 + nanospheres could be obtained via PAA assisted hydrothermal process at the pH value of 4–5 and 160 °C for 1–4 h. The obtained product is a mixture of α and β phases NaYF 4 :Yb 3 + , Er 3 + when the reaction time increased from 5 to 24 h. When the solution was treated at 200 °C for 8–24 h at the pH value of 8–9, pure β -NaYF 4 :Yb 3 + , Er 3 + microspheres were obtained. The FE-SEM results showed that the morphology of pure α -NaYF 4 :Yb 3 + , Er 3 + was spherical nanoparticles with an average diameter of about 70 nm and the pure β -NaYF 4 :Yb 3 + , Er 3 + was microsphere with an average diameter of about 700 nm, which is composed of spherical nanoparticles (30 nm). The FT-IR and UV spectra showed that BSA had been conjugated with α -NaYF 4 :Yb 3 + , Er 3 + upconversion phosphors. The luminescence properties of α -( β -) NaYF 4 :Yb 3 + , Er 3 + phosphors were also studied. Under 980 nm excitation, the upconversion luminescence (UCL) intensity of β -NaYF 4 :Yb 3 + , Er 3 + microspheres was much stronger than that of α - NaYF 4 :Yb 3 + , Er 3 + nanoparticles. Moreover, both red and green upconversion were ascribed to the two-photon process.
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL PAPERS
·
2014
·Vol. 68
·期 11
·1521-1531
·
IF 1.193
Wang Qian-Wen,Liu Jia-Jia,Liu Xiong,Zha Hong-Wei,Ren Na
A high-efficient purification method of flavonoids from Lithocarpus polystachyus Rehd leaves is reported. Two adsorbents functionalized with glucosamine (GA) and β -cyclodextrin ( β -CD) were synthesized and characterized by the Fourier transform infrared (FTIR) spectra. Also, adsorption properties and purification effect of flavonoids on different adsorbents were investigated. Two typical commercial adsorbents, AB-8 and D101, were employed as the reference materials. The adsorption capacity was enhanced remarkably by the introduction of GA and β -CD groups to the adsorbents. Moreover, the isotherms were well fitted by the Freundlich model. After a one-step column chromatographic separation, the purity of phloridzin increased significantly from 14.65 % to 79.48 % for PS-CD, to 66.56 % for PS-GA, 44.68 % for D101, and to 52.84 % for AB-8. This study provides a novel alternative matrix for the purification of phloridzin from Lithocarpus polystachyus Rehd leaves extracts.
✓ 已验证
产品关联
DOI
高影响因子
MATERIALS RESEARCH BULLETIN
·
2013
·
IF 1.913
Lin Gan,Zhi-Yong Mao,Xiong-Hui Zeng,Yu-Qiang Zhang,Yang Zhao,Fang-Fang Xu,Ying-Chun Zhu,Xue-Jian Liu
Eu 2+ -doped SiAlON phosphors with the composition of Eu x Si 6− z Al z O z N 8− z (0.5 ≤ z ≤ 3) at a fixed x = 0.01 were synthesized by the gas pressure sintering method. Dependence of luminescence properties on the phase compositions in β-SiAlON:Eu 2+ phosphors has been examined via fluorescence microscope and scanning electron microscope equipped with a cathodoluminescence spectrometer and an energy dispersive spectrometer. Bimodal emission (green and violet) from β-SiAlON phase is observed in the samples with z ≥ 2, indicating co-existence of two different kinds of coordination for Eu 2+ ions in the host lattice.
✓ 已验证
产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2014
·
IF 4.28
Ping Shao,Jiafeng Zhang,Zhongxian Fang,Peilong Sun
Chlorogenic acid (CGA) is a polyphenol commonly existed in fruits and vegetables with a variety of bioactivities. To increase its stability and potential broader industrial application, CGA/cyclodextrin (CD) complexes were prepared using β-cyclodextrin (β-CD) and its derivative (2-hydroxypropyl)-β-cyclodextrin (2-HP-β-CD). Their inclusion complexation behavior and characterization were investigated by FT-IR, XRD, SEM, and 1 H NMR spectroscopy. The morphologic and spectral studies indicated the formation of inclusion complexes. Moreover, spatial arrangements of inclusion complexes were proposed based on 1 H NMR results. The antioxidant activities of the complexes were improved compared with the CGA without inclusion, and the CGA/HP-β-CD complexes showed the highest antioxidant activity. In addition, the CGA/CD complexes were added in grape juice to explore the potential on the preservation of anthocyanin and color quality. Results showed that the degradation of anthocyanins was reduced when CGA and CGA/CDs inclusions were added in grape juice due to copigmentation effect, and their copigmentation strength showed in an order of CGA/HP-β-CD complex > CGA/β-CD complex > free CGA.
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2013
·Vol. 3
·期 31
·12726-12734
·
IF 2.562
Yanying Wu,Zhiyu Dou,Ying Liu,Guojun Lv,Tao Pu,Xingquan He
A stable electroactive film of β-cyclodextrin-poly(N-isopropylacrylamide) (CD-PNIPAM) was successfully prepared on a glassy carbon electrode (GCE) surface using an electroadsorption method for the determination of dopamine (DA). The electrochemical behavior of the fabricated sensor towards the catalytic oxidation of DA was investigated by a cyclic voltammetry (CV) method in pH 5.0 phosphate buffer solution. The CD-PNIPAM modified electrode exhibited a good electrocatalytic activity towards the oxidation of dopamine, and led to a significant decrease in the anodic overpotentials compared with bare GCE. Using a differential pulse voltammetry (DPV) method, the sensor gave a linear response to DA over the concentration range 0.1–60 μM with a detection limit (S/N = 3) of 3.34 × 10−8 M. The sensitivity of the sensor was 564.2 μA mM−1. It was found that CD-PNIPAM possessed an inclusive property resulting from the β-CD in the structure of the polymer and could form an inclusion complex with dopamine molecules by hydrogen bonding interactions, which improved the detection limit and sensitivity for DA. The proposed sensor exhibits good sensitivity, selectivity and stability and has shown potential for the determination of DA in real samples.
✓ 已验证
产品关联
DOI
高影响因子
ELECTROPHORESIS
·
2014
·
IF 3.161
Ying Feng,Tingting Wang,Zhengjin Jiang,Bezhan Chankvetadze,Jacques Crommen
A series of eight chiral β-blocker drugs, acebutolol, atenolol, carazolol, carteolol, carvedilol, propranolol, sotalol, and talinolol, have been enantioseparated using two single-component anionic β-CD derivatives, namely heptakis (2,3-di- O -methyl-6-sulfo)-β-CD (HDMS-β-CD) and heptakis (2,3-di- O -acetyl-6-sulfo)-β-CD (HDAS-β-CD), in aqueous CE and NACE. The influence of the nature of substituents (methyl or acetyl) in positions 2 and 3 on the CD derivatives and of the electrophoretic medium (water or methanol) on the enantioselectivity and enantiomer affinity pattern (EAP) of these structurally related compounds was systematically studied. All eight β-blockers could be enantioseparated at least partially in the four CE systems, except sotalol with HDMS-β-CD in NACE. In general, lower affinity and enantioselectivity were obtained in the presence of HDMS-β-CD compared to HDAS-β-CD. Reversals of EAPs were observed for all compounds. EAPs toward these two CDs were found to be opposite to each other in NACE for all compounds except carvedilol and in aqueous CE for atenolol, carteolol, talinolol, and sotalol. It is particularly noteworthy that opposite EAPs were also observed using the same CD derivative when the aqueous BGE was replaced with the methanolic one: for carazolol, carvedilol, and propranolol in the presence of HDMS-β-CD and for acebutolol and carvedilol with HDAS-β-CD.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2014
·Vol. 6
·期 24
·9684-9690
·
IF 1.938
Xu Chen,Yong Luo,Bo Shi,Xianming Liu,Zhigang Gao,Yuguang Du,Weijie Zhao,Bingcheng Lin
β-Agonists are a group of illegal but widely used feed additives in the stockbreeding industry. In order to achieve simple-to-use, fast and high-throughput testing of this banned chemical, herein we suggest a paper-based analytical device on which a chemiluminescence diminishment method was performed. In this approach, extracts from swine hair samples as well as luminescent reagents, such as luminol and potassium periodate solution, in a low volume were applied to our device. It was found that the light emission was diminished by the β-agonists extracted from the swine hair samples. The degree of diminishment is proportional to the concentration of the β-agonists from 1.0 × 10−5 to 1.0 × 10−8 mol L−1. Also, the concentrations of solutions for chemiluminescence were optimized. The mechanism and reaction kinetics of chemiluminescence were discussed as well. The detection limit was obtained as 1.0 × 10−9 mol L−1, and recoveries from 96% to 110% were achieved, both of which suggested that our method will be favourable in field applications for swine hair samples.
✓ 已验证
产品关联
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2015
·
IF 2.711
Congli Wang,Lizhen Huang,Shengmei Song,Bassam Saif,Yehong Zhou,Chuan Dong,Shaomin Shuang
The β-cyclodextrin assemblied magnetic Fe 3 O 4 nanoparticles (β-CD-MNPs) were successfully fabricated via a layer-by-layer method. Possessing an average size 14 nm, good stability and super-paramagnetic response (Ms 64 emu/g), the resultant nanocomposites could be served as a versatile biocompatible platform for selective loading, targeted delivery and pH-responsive release of stereoisomeric doxorubicin (DOX) and epirubicin (EPI). 1 H-nuclear magnetic resonance ( 1 H NMR) and the computer simulation further give the evidence that partial anthracene ring of drug molecule is included by β-CD. In addition, non-toxic β-CD-MNPs have excellent biocompatibility on MCF-7 cells, and cellular uptake indicate that different amounts of DOX or EPI can be transported to targeting site and released from the internalized carriers. The results demonstrate that as-prepared β-CD-MNPs could be a very promising vehicle for DOX and EPI.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）