Advanced Biology
·
2025
·
IF 2.6
Xi-Ting Lv,Wen-Xiu Yang,Xiao Zhang,Bao-Ling Li,Wen-Ying Wang,Guo-En Wang
Guilongwan (GLW), a representative of traditional Chinese Medicine (TCM) has been utilized to treating diabetic foot ulcer (DFU)-related syndrome including an intolerance of cold with cold limbs, blood circulation disorder, and immune dysfunction for decades. However, the chemical and biological mechanisms of GLW remain unclear. This study aims to discover the biological mechanisms of GLW on DFU by using streptozotocin- and skin-puncher-induced DFU rat models, in vitro macrophage models, and in silico analysis. The alterations in pathology, Notch1 signaling, and macrophage polarization are detected. The results indicated that GLW promoted wound healing, cutaneous cell proliferation, and angiogenesis in DFU rats by inhibiting delta-like (DLL) 4/Notch1 signaling. In addition, GLW inhibited M1 polarization and promoted M2 polarization in diabetic wounds. Seventeen chemical compounds in GLW-medicated serum are identified. In silico analysis and in vitro experiments demonstrated that GLW-medicated serum and its main compounds inhibited the expression of DLL4 in matrix metalloproteinase-9-induced M1 macrophages. In conclusion, GLW ameliorated experimental DFU rats via the inhibition of DLL4/Notch1 signaling in M1 macrophages. This study provided a new biologic mechanism for GLW in the treatment of DFU.
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PubMed
高影响因子
ACS Applied Nano Materials
·
2025
·
IF 5.5
Caiwen Pan,Qinghong Shi,Xiaoyan Dong,Linling Yu,Yan Sun
Detoxification of the typical organophosphate pesticide methyl parathion (MP) is of great significance for environmental remediation and sustainable development. Hydrolysis of MP using organophosphate hydrolase (OPH) is an effective and environmentally friendly way to degrade MP, but the hydrolytic product, 4-nitrophenol (4-NP), is still toxic. The cascade conversion of MP to 4-NP followed by reduction to a low toxic product, 4-aminophenol (4-AP), is thus an attractive way to detoxify MP. Herein, we fabricated a highly active reduction-catalytic cupric metal–organic framework (MOF) (Cu-AZO), which exhibited over 6-fold higher activity than the previous Cu-AZB. Thus, a chemoenzymatic catalyst (OPH/Cu-AZO) for one-pot cascade MP conversion to 4-AP was constructed by in situ immobilization of the OPH in Cu-AZO. OPH/Cu-AZO displayed an enzyme loading capacity of 16–69 mg/g with an activity recovery of up to 252%, and an average cascade conversion rate of up to 41.7 μmol·min–1·gcatalyst–1. At the optimized conditions, OPH/Cu-AZO completely converted 50 μM MP to 4-AP within 3 min at a low reductant concentration (10 mM NaBH4) in the one-pot cascade reaction. Moreover, the hybrid catalyst retained 99% activity in 10 days storage and >65% activity in 10 recycles. The results proved that OPH/Cu-AZO was the most efficient chemoenzymatic catalyst reported so far in the cascade conversion of MP to 4-AP, underscoring its potential applications in the detoxification of MP.
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CRYSTAL GROWTH & DESIGN
·
2025
·
IF 3.4
Feiyan Xie,Junqiang Gu,Jie Zhang,Yintong Chen,Run Yin,Junhao Li
Due to the lack of red light, currently available commercial phosphor-converted white light-emitting diodes (pc-WLEDs) have a poor color rendering index. In order to improve the color rendering performance of pc-WLEDs, it is urgent to develop satisfactory bright red emission phosphors. Herein, we reported a bright red luminescent Na5Lu(WO4)4:Sm3+ phosphor. By studying the effect of Sm3+ doping concentration on luminescence performance, it was found that the concentration quenching effect was attributed to the dipole–dipole interaction. The quantum yield, color purity, and CIE coordinates of the optimal Sm3+ concentration activated Na5Lu(WO4)4:9%Sm3+ phosphor were 49.37%, 95.12%, and (0.653, 0.346), respectively. Furthermore, the phosphor had strong resistance to thermal quenching, and the luminous intensity at 425 K was only reduced by 10.20% compared with that at 300 K, while maintaining excellent color stability (Δε = 9.27 × 10–3). Finally, we used Na5Lu(WO4)4:9%Sm3+ phosphor as the red component, and additionally added commercial blue and green phosphors, combined with the 365 nm near-ultraviolet chip to produce a white LED device with a CRI of up to 90.5 and a CCT as low as 4581 K. These results indicated that Na5Lu(WO4)4:Sm3+ phosphors had broad potential applications in high color rendering index white LEDs excited by near-ultraviolet light.
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COMBUSTION AND FLAME
·
2020
·
IF 4.57
Qiang Lu,Wen-luan Xie,Bin Hu,Ji Liu,Wei Zhao,Bing Zhang,Ti-peng Wang
Pyrolysis of lignin involves complex unimolecular decomposition and bimolecular interaction mechanisms. Unimolecular decomposition of lignin model compounds has been widely investigated, while bimolecular interactions are far from well elucidated. Currently, only two radical reaction-based interactions, i.e., hydrogen abstraction and hydrogen-bond-induced homolysis mechanisms, have been proposed to explain the interactions in the lignin pyrolysis process, which ignores the concerted reaction-based interactions involving the pyrolytic products of lignin. Particularly, a large number of phenolics are produced in the pyrolysis of lignin, which can further affect the concerted pyrolysis reactions of lignin. In the present work, a novel interaction mechanism, phenolics-assisted hydrogen transfer (phenolics-AHT) process for the concerted reactions, is confirmed and carefully investigated. Phenylethyl phenyl ether (PPE), a typical β -O-4 lignin dimer, is used as the model compound to reveal the phenolics-AHT mechanism in the lignin pyrolysis system. Density functional theory (DFT) calculation, electrostatic potential (ESP) analysis, and fast pyrolysis experiment results are combined to reveal that the phenolic products participate in the hydrogen transfer process of the concerted Maccoll elimination reaction by means of their phenolic hydroxyl structures and significantly reduce the energy barrier, thus promoting the cleavage of the β -O-4 linkage. On the contrary, phenolics-AHT has no obvious effect on the concerted retro-ene fragmentation. At medium pyrolysis temperatures, the hydrogen transfer processes induced by phenolic products play a more significant role in the dissociation of the β -O-4 linkage than the hydrogen abstraction induced by radicals, due to the limited amount of free radicals . In addition, DFT calculation results further suggest that decarbonylation , decarboxylation , dehydration, tautomerism , and other concerted reactions can also be promoted by phenolics-AHT.
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高影响因子
Current Drug Delivery
·
2015
·Vol. 12
·期 6
·726-735
·
IF 1.478
Chenhui Wang,Changhui Yu,Kongtong Yu,Lesheng Teng,Jiaxin Liu,Xuesong Wang,Fengying Sun,Youxin Li
The use of biodegradable polymers such as PLGA to encapsulate therapeutic proteins for their controlled release has received tremendous interest. However, an acidic environment caused by PLGA degradation productions leads to protein incomplete release and chemical degradation. The aim of this study was to develop novel PCADK/PLGA microspheres to improve protein stability and release behavior. Bovine serum albumin (BSA) incubated in PCADK and PLGA degradation products was investigated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), size exclusion chromatography (SEC-HPLC), circular dichroism (CD) and fluorescence spectroscopy. Blended microspheres of PCADK/PLGA were prepared in different ratios and the release behaviors of the microspheres and the protein stability were then measured. The degradation properties of the microspheres and the pH inside the microspheres were systematically investigated by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) to examine the mechanism of autocatalytic degradation and protein stability. BSA was more stable in the presence of PCADK monomers than it was in the presence of PLGA monomers, revealing that PCADK is highly compatible with this protein. PCADK/PLGA microspheres were successfully prepared, and 2/8 was determined to be the optimal ratio. Further, 43% of the BSA formed water-insoluble aggregates in the presence of PCADK/PLGA microspheres, compared with 57% for the PLGA microspheres, demonstrating that the BSA encapsulated in PCADK/PLGA blended microspheres was more stable than in PLGA microspheres. The PCADK/PLGA blended microspheres improved protein stability and release behavior, providing a promising protein drug delivery system.
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高影响因子
Nanoscale
·
2018
·Vol. 10
·期 9
·4500-4514
·
IF 7.233
Xin Liang,Lei Shen
In2O3(ZnO)k natural superlattices (where k is an integer), consisting of relatively earth abundant and non-toxic elements with coarsening-resistant nanostructures, are environmentally friendly materials with the potential for high temperature thermoelectric applications. Herein, we report our investigation of the high temperature thermoelectric properties of the In2O3(ZnO)4 superlattice bulk polycrystals that were singly and dually doped with Al and Ce. Transport property measurements revealed that Al and Ce did not only enter the ZnO blocks but also modified the InO2 single atomic layers. The effective electron potential barrier height of the superlattice interfaces can be adjusted by doping, and the optimal value that maximizes the power factor is of the order of kBT above the Fermi level. The interfacial thermal (Kapitza) resistance of the InO2 atomic sheets dramatically increased with doping, primarily accounting for the bulk thermal conductivity reduction. At the optimal crossing of the interfacial thermal resistance and the effective potential barrier height, a maximum ZT of ≈0.22 was achieved at 800 °C in the 1.6 mol% Al-doped superlattice, which was an enhancement of ∼200% over the pristine In2O3(ZnO)4. This work provides a new perspective on enhancing the high temperature thermoelectric performance of nanostructured oxides by synergistically optimizing the interfacial phonon and electron transport properties.
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INORGANIC CHEMISTRY
·
2021
·
IF 5.165
Jun Sun,Tushagu Abudouwufu,Congcong Jin,Zhiyong Guo,Min Zhang
A new alkali-metal iodate, K6(IO6H4)(HI2O6)(HIO3)2(IO3)4·2H2O (KIOH), was successfully grown at room temperature by a slow evaporation method. To our knowledge, the title compound is the first alkali-metal iodate containing isolated [I5+O3] and [I7+O6] units in one structure. Both the bond valence sum and X-ray photoelectron spectroscopy confirmed this phenomenon, which is consistent with the single-crystal data. Also, the theoretical calculation results showed that the title compound is a potential birefringent material. What is more, the low-cost growth of centimeter-sized crystals for the title compound greatly enriches the structural chemistry of the iodate system.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2026
·
IF 9.1
Jiaxi Jin,Yuyang Xiao,Hao Lai,Ruixing Fu,Peilun Shen,Dianwen Liu,Xinyi Liang,Bo Peng
The efficient separation of chalcopyrite from sphalerite remains a significant challenge in flotation due to their similar surface properties. In this study, Trisodium 4-carboxy-5-mercapto-3-hydroxy-isothiazole (TCMHI) was introduced as a novel, eco-friendly, and selective organic depressant for sphalerite. Micro-flotation tests demonstrated that TCMHI (73 mg/L) could selectively depress sphalerite flotation (recovery <14%) at pH 7.0 while maintaining high chalcopyrite recovery (> 94%). Successful separation was achieved in artificially mixed mineral tests, yielding a concentrate with a Cu grade of 32.01% and a recovery of 96.65%. To validate its industrial applicability, bench-scale flotation tests were conducted on a real Cu Zn sulfide ore. The results showed that TCMHI achieved metallurgical performance comparable to the conventional depressant zinc sulfate (ZnSO 4 ), maintaining a copper recovery of 71.32% while effectively suppressing zinc recovery to 21.18% in the concentrate. Surface characterization techniques, including contact angle, zeta potential, and Atomic Force Microscopy (AFM), revealed that TCMHI preferentially adsorbs onto the sphalerite surface, forming a dense hydrophilic layer that hinders collector adsorption. X-ray Photoelectron Spectroscopy (XPS) and Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) analyses confirmed that TCMHI chemically adsorbs on sphalerite through the formation of stable Zn-TCMHI surface complexes. Density Functional Theory (DFT) calculations further elucidated the mechanism, showing that TCMHI forms a stable five-membered chelate ring with surface Zn atoms via its nitrogen and endocyclic sulfur atoms. Crucially, the adsorption energy of TCMHI on sphalerite (−209.76 kJ/mol) was found to be significantly more negative than that of the collector (−95.88 kJ/mol), explaining its superior depression performance. This work provides a promising reagent for the sustainable and efficient flotation separation of Cu Zn sulfide ores.
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.7
Rui-Lin Zhang,Jia-Qing Wang,Sara Taha Abdelkhalek,Shan-Cheng Yi,Yuping Zha,Man-Qun Wang
The olfactory system is pivotal for insect behavior, with odorant-binding proteins (OBPs) serving as primary detectors for semiochemicals. Previous studies demonstrated that DhelOBP4 in Dastarcus helophoroides is essential for detecting herbivore-induced plant volatiles (HIPVs). Here, we demonstrated through dual-luciferase reporter assays, yeast one-hybrid (Y1H) assays, and electrophoretic mobility shift assays (EMSA) that transcription factors BarH1/2 directly activate DhelOBP4 expression by binding to its promoter. RNAi knockdown revealed sex-specific regulation: BarH1 silencing significantly reduced DhelOBP4 expression only in females, whereas BarH2 knockdown affected both sexes. Y-tube olfactometer assays showed that BarH1 depletion abolished female attraction to α-pinene and 2-undecyloxy-1-ethanol, as well as male attraction to α-pinene. BarH2 knockdown eliminated attraction in both sexes and reversed the female response to α-pinene from attraction to repulsion. These findings establish DhelOBP4 as a key detector of these volatiles, under sex-specific regulation by BarH1/2 to modulate behavior, revealing novel mechanisms in tritrophic interactions.
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PubMed
高影响因子
ORGANIC & BIOMOLECULAR CHEMISTRY
·
2026
·
IF 2.8
Lin Chen,Shuang Xu,Zhiming Chen
Direct α-C(sp3)−H arylation of 4-aminopyrazolones involving arynes, enabled by dual phase-transfer catalysis and ultraviolet irradiation, has been developed. The dual phase-transfer catalytic pathway powered by triaminocyclopropenium salts gave rise to the generation of structurally diverse 4-amino-4-arylpyrazolones in moderate to excellent yields, while the photochemical protocol furnished the products with moderate yields. Notably, these products are tentatively proposed as candidates for carbamate pesticides based on the preliminary structural analysis. The synthetic potentials of the methods were shown by gram-scale reactions, and the possibilities for greater structural diversity of the product were highlighted by derivatizations. The results of control experiments indicated that the timely capture of the transient arynes by the activated 4-aminopyrazolone nucleophiles is the key to the success of the reactions.
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PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2026
·
IF 11.3
Guiping Liu,Xin Yu,Xuya Wu,Dexuan Tang,Shengqiang Ying,Shui Wang,Jiandong Jiang,Wenjing Qiao
4-Hydroxy-chlorothalonil (4-OH-TPN), the main metabolite of the widely used fungicide chlorothalonil, has recently been reported to be reductively dechlorinated by an anaerobic consortium containing Dehalogenimonas. While the pure culture of the Dehalogenimonas has not been isolated and the reductive dehalogenase genes (rdhAs) responsible for the 4-OH-TPN dechlorination remain unclear. The assembled genome of Dehalogenimonas contained a 1,2-dichloroethane (1,2-DCA) reductive dehalogenase gene (dcpA) ortholog. In this study, by alternating the substrate from 4-OH-TPN to 1,2-DCA, a pure culture of Dehalogenimonas, designated as 4OHTPN1, was finally isolated. Strain 4OHTPN1 potentially represents a novel species in the genus Dehalogenimonas and contains 33 rdhA genes and 4 rdhB genes. The novel rdhA26 gene (designated htpnA) was identified as the key gene involved in 4-OH-TPN dechlorination. Its transcriptional and protein expression levels were 25.0-fold and 10.2-fold higher than the average of all rdhA genes when the culture was solely amended with 4-OH-TPN. In contrast, the dcpA ortholog, rdhA25, exhibited 10.1-fold and 9.8-fold higher transcriptional and protein expression levels when only amended with 1,2-DCA, highlighting a substrate-specific regulatory response to a distinct electron acceptor. This study expands our knowledge of the rdhA gene for 4-OH-TPN dechlorination in the pure culture of Dehalogenimonas.
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高影响因子
JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
·
2020
·
IF 3.905
Qiang Lu,Guan Zhang,Zhen-xi Zhang,Hu Bin,Kai Li
A novel way was put forward with 9,10-dihydroanthracene (DHA) as a solid hydrogen donor to improve the selective production of 4-ethyl phenol (4-EP) from catalytic fast pyrolysis (CFP) of bagasse over activated carbon (AC) catalyst. Both pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) and lab-scale CFP experiments were carried out on the 4-EP formation affected by various factors, in order to determine the optimal conditions for 4-EP production. The results indicated that DHA exhibited promising hydrogenation capabilities to improve 4-EP formation. In Py-GC/MS test, the 4-EP reached a maximal yield of 3.21 wt% with its corresponding selectivity of 22.96 % at 350 °C under AC-to-bagasse (AC-to-BA) and DHA-to-bagasse (DHA-to-BA) ratios of 6:1 and 1:5, respectively. Whereas, the maximal 4-EP yield was only 2.18 wt% in the absence of DHA, indicating a yield increase of 47.2 % by DHA. Moreover, lab-scale experiments attained the highest 4-EP yield and the corresponding selectivity of 3.05 wt% and 16.31 % with DHA, compared with the yield of 1.67 wt% without DHA. In addition, the possible hydrogenation mechanism of DHA in facilitating 4-EP formation was revealed.
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