ANALYST
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2024
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IF 3.6
Binbin Chen,Lizhu Fan,Chunyu Li,Lu Xia,Kaiwen Wang,Jinshu Wang,Dawei Pang,Zhouhao Zhu,Peijie Ma
In this work, Au/Bi2O3 was synthesized by loading Au nanoparticles (NPs) onto β-Bi2O3 by a simple solution reduction method. β-Bi2O3 was synthesized by a precipitation-thermal decomposition procedure, which results in significantly improved SERS detection limits down to 10−9 M for methylene blue (MB) and 10−7 M for methyl orange (MO) as probe molecules, comparable to those reported for the best semiconductor SERS substrates. In particular, further deposition of Au NPs (5.20% wt%) onto β-Bi2O3 results in a two-order-of-magnitude enhancement in detection sensitivity, achieving a detection limit of 10−11 M for MB and 10−9 M for MO. Under ultraviolet/visible irradiation, the Au/Bi2O3 hybrids substrate exhibits superior self-cleaning ability due to its photocatalytic degradation ability which can be applied repeatedly to the detection of pollutants. The advanced composite substrate simultaneously achieved ultra-low mass loading of Au NPs, outstanding detection performance, good reproducibility, high stability and self-cleaning ability. The development strategy of low load noble metal coupled high performance semiconductor β-Bi2O3 to obtain nano-hybrid materials provides a method to balance SERS sensitivity, cost effectiveness and operational stability, and can be synthesized in large quantities, which is a key step towards commercialization and has good reliability prospects.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
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IF 13.3
Tiancheng Li,Mengmeng Shu,Cheng Zhu,Yixin Li,Qicheng Liu,Zhiyu Zhu,Yangyang Jiang,Peng Mei,Yu Jin,Bing Fang,Lunguo Xia
In osteoarthritic chondrocytes, dysregulation of mitochondrial dynamics due to excessive intracellular reactive oxygen species (ROS) under inflammatory conditions is a significant contributory factor to poor treatment outcomes, because it leads to a reduced cell response to chondrogenic drugs during this state. The aim of this study was to develop a multifunctional Au@CeO 2 yolk-shell nanozyme (YSN) as a novel therapeutic nanomaterial and drug delivery system. Under photothermal conditions, Au@CeO 2 nanozymes neutralized excessive ROS, enhanced antioxidant capacity, and maintained mitochondrial homeostasis of osteoarthritic chondrocytes, thereby restoring cellular responsiveness to external stimuli. Furthermore, bioactive peptide CK2.1 loaded in YSNs (Au@CeO 2 -CK2.1) achieved ordered release under photothermal conditions, effectively promoting chondrocyte anabolic activity. In a mice osteoarthritis (OA) model, local Au@CeO 2 -CK2.1 photothermal therapy effectively enhanced cartilage repair and regeneration in vivo . Mechanically, our research uncovers that decreased chondrogenic drug response is associated with activation of extracellular regulated kinase-1 and -2 (ERK1/2) pathway, and Au@CeO 2 -CK2.1 YSNs regulates mitochondrial dynamics through inhibiting ERK1/2 and dynamin-1-like protein (DRP1) phosphorylation. The results of this study demonstrated an effective therapeutic strategy that rescued chondrogenic drug response in osteoarthritic chondrocytes, achieving anti-inflammation and cartilage regeneration.
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Science China-Chemistry
·
2010
·Vol. 53
·期 7
·1497-1501
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IF 0
Ma Hong,Nie Xin,Cai JiaYing,Chen Chen,Gao Jin,Miao Hong,Xu Jie
The catalytic oxidation of biomass-derived polyhydroxyl compounds provides an efficient and green route for biomass resource utility. Gold nanocrystals supported on Mg(OH) 2 were synthesized by sol-immobilization method, characterized by XRD, TEM, UV-vis DRS, and tested in the selective oxidation of 1,2-propanediol to lactic acid. The gold particle size and the ratio of Au to 1,2-propanediol strongly influenced the catalytic activity. Over Au/Mg(OH) 2 with the majority of gold particles concentrated in 14–18 nm, excellent catalytic performance with 94.4% conversion and 89.3% selectivity to lactic acid has been achieved under 0.3 MPa O 2 at 60 °C for 6 h.
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SENSORS AND ACTUATORS B-CHEMICAL
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2024
·
IF 8
Honglian Wu,Ruolin Zhang,Huang Lai,Luogen Lai,Yi Liu,Yadi Fan,Yu Zhang,Jingyu Shi,Mo Yang
Endotoxins or lipopolysaccharides (LPS) are potent inflammatory agents that pose serious health risks even at low concentrations, thus requiring sensitive and reliable detection methods. Current methods for endotoxin detection, primarily the Limulus Amebocyte Lysate (LAL) assay and Enzyme-linked Immunosorbent Assays (ELISA) are limited by complex procedures, prolonged assay time, and susceptibility to interference. To address these challenges, we developed a single-reporter probe-based dual-mode biosensor integrating MXene-Au nanosheets with the CRISPR/Cas12a system for endotoxin detection. The sensing platform was constructed by conjugating ssDNA-Cy5 reporters onto MXene-Au nanosheets. MXene-Au nanosheets served dual functions, including efficient quenching of fluorescence and strong enhancement of SERS signals. In the presence of target endotoxins, CRISPR/Cas12a remains inactive, leaving the ssDNA-Cy5 reporters intact on the MXene-Au surface. The close proximity of Cy5 to the MXene-Au surface results in quenched fluorescence (“FL off”) but enhanced SERS signals (“SERS on”). Conversely, in the absence of endotoxins, activated CRISPR/Cas12a cleaves the ssDNA-Cy5, releasing Cy5 from the surface, thus leading to fluorescence recovery (“FL on”) but diminished SERS signals (“SERS off”). By measuring the changes in SERS and FL signals and analysing the ratiometric I SERS /I FL signals, the biosensor achieved a lower limit of detection (LOD) of 15.9 pg/mL within 30 minutes compared to single detection modes. Validation studies in complex matrices, including spiked water and milk samples, showed performance comparable to commercial endotoxin detection kits. Overall, our CRISPR-Cas12a-mediated fluorescent-SERS dual-mode biosensor not only improves detection sensitivity but also provides built-in quality control, improving the reliability of results through cross-validation.
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TALANTA
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2024
·
IF 5.6
Xiatong Wang,Shaohe Chang,Xiaoling Zhang,Xuhua Guo,Yongjun Xu,Di Yang,Linpin Luo,Jiamin Wang,Haofen Wu,Yan Cui,Jianlong Wang,Yanwei Ji
The conventional lateral flow immunoassay (LFIA) based on gold nanoparticles (Au NPs) is limited by low sensitivity due to the insufficient brightness of Au NPs. To address this problem, noble metal nanomaterials with localized surface plasmon resonance (LSPR) and synthetic tunability are potential signal outputs for LFIA, which can achieve better optical properties by adjusting the preparation conditions. Herein, this study prepared the hollow silver/gold nano-stars (HAg/Au NSts) as LFIA signal output via the galvanic replacement method. HAg/Au NSts with anisotropic hollow alloy nanostructures exhibit a wide visible light absorption band and great NIR thermal conversion efficiency (η = 37.32%), which endows them with enhanced colorimetric and photothermal signals. Further, we constructed a colorimetric-photothermal (CM-PT) dual-signal HAg/Au NSts-LFIA and chose staphylococcal enterotoxin B as the target analyte. The linear range of HAg/Au NSts-LFIA is 0.19 – 100 ng mL -1 , and the limit of detection (LOD) is up to 0.29 ng mL -1 and 0.09 ng mL -1 in the colorimetric and photothermal modes respectively. Compared with the conventional Au NPs-LFIA, HAg/Au NSts-CM/PT-LFIA effectively improved the detection performance of LFIA. In addition, HAg/Au NSts-LFIA also showed satisfactory sensitivity (vLOD = 0.78 ng mL -1 ) and recovery (89.06 - 114.74%) in milk and pork samples. Therefore, this work provides a new shape design idea for noble metal nanomaterials in biosensor applications.
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高影响因子
SENSORS
·
2011
·Vol. 11
·期 8
·7749-7762
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IF 1.774
Ning Gan,Jianguo Hou,Futao Hu,Yuting Cao,Tianhua Li,Zhiyong Guo
An ultrasensitive and renewable electrochemiluminescence (ECL) immunosensor was developed for the detection of tumor markers by combining a newly designed trace tag and streptavidin-coated magnetic particles (SCMPs). The trace tag (RuL@SiO2-Au∼RuL-Ab2) was prepared by loading Ru(bpy)32+(RuL)-conjuged secondary antibodies (RuL-Ab2) on RuL@SiO2(RuL-doped SiO2) doped Au (RuL@SiO2-Au). To fabricate the immunosensor, SCMPs were mixed with biotinylated AFP primary antibody (Biotin-Ab1), AFP, and RuL@SiO2-Au∼RuL-Ab2 complexes, then the resulting SCMP/Biotin-Ab1/AFP/RuL@SiO2-Au∼RuL-Ab2 (SBAR) sandwich-type immunocomplexes were absorbed on screen printed carbon electrode (SPCE) for detection. The immunocomplexes can be easily washed away from the surface of the SPCE when the magnetic field was removed, which made the immunosensor reusable. The present immunosensor showed a wide linear range of 0.05–100 ng mL−1for detecting AFP, with a low detection limit of 0.02 ng mL−1(defined as S/N = 3). The method takes advantage of three properties of the immunosensor: firstly, the RuL@SiO2-Au∼RuL-Ab2 composite exhibited dual amplification since SiO2could load large amount of reporter molecules (RuL) for signal amplification. Gold particles could provide a large active surface to load more reporter molecules (RuL-Ab2). Accordingly, through the ECL response of RuL and tripropylamine (TPA), a strong ECL signal was obtained and an amplification analysis of protein interaction was achieved. Secondly, the sensor is renewable because the sandwich-type immunocomplexes can be readily absorbed or removed on the SPCE’s surface in a magnetic field. Thirdly, the SCMP modified probes can perform the rapid separation and purification of signal antibodies in a magnetic field. Thus, the present immunosensor can simultaneously realize separation, enrichment and determination. It showed potential application for the detection of AFP in human sera.Keywords:[Ru(bpy)3]2+@SiO2-Au;alpha-fetoprotein;sandwich-type immunoreaction;screen printed carbon electrode;electrochemiluminescence
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高影响因子
ELECTROCHIMICA ACTA
·
2017
·
IF 4.798
Rong Wang,Shengxia Zhang,Yun Su,Junxia Liu,Ye Ying,Feng Wang,Xiaowei Cao
A semi-solid state electrochromic device with deep eutectic solvent gel as the electrolyte, Ethyl-viologen as the electrochromic compound, and K 4 Fe(CN) 6 as the electron donor was reported. Four kinds of silica were simply mixed with ethaline for preparing the gel electrolyte, and the hydrophilic ones were found to be capable of being used as the gelling agent . To enhance the electron transfer at the electrode/gel interface and keep the transparency of the electrode, tiny amount of Au was electrochemically deposited on the FTO electrode. The as-prepared electrochromic device shows a coloration time of ca. 60 s at −0.7 V with the coloration efficiency of 91 cm 2 /C, and a bleaching time of ca. 150 s at 0 V with the efficiency of 194 cm 2 /C. The reversible response of the electrochromic device up to 820 cycles with Δ T max > 60% was also demonstrated.
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RUSSIAN JOURNAL OF APPLIED CHEMISTRY
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2024
·
IF 0.6
Li Hui,Shen Dongxue,Yang Zhe,Ji Guojia,Zhang Zhiwei,Lu Xulin,Zhang Jingmei,Zhao Zhongbao
Abstract Au nanoparticles loaded on biochar (PDA-BC/Au) was prepared using adhesion and reduction properties of polydopamine by in-situ reduction method. PDA-BC/Au was characterized by the specific surface area analyzer (BET), the scanning electron microscope (SEM), the transmission electron microscopy (TEM), X-ray diffractometer (XRD), X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectrometry (FTIR). Analysis results prove the successful synthesis of composites decorated with Au nanoparticles in the biochar matrix coating with polydopamine layer. PDA-BC/Au was employed as an adsorbent to remove the cationic dyes (MB and MG) from aqueous solution. The tests confirm that PDA-BC/Au can remove up to 99.0% of MB within 30 min and achieve removal rate over 97.2% of MG after 60 min adsorption. Adsorption kinetics and isotherm models reflect that the adsorption of dyes by PDA-BC/Au has a better fit with pseudo-secondary kinetic equation and the Langmuir adsorption model, displaying chemisorption-dominated single-molecule layer adsorption mechanism. Additionally, after five recycling cycles, the removal rates of PDA-BC/Au for MB and MG only dropped by 3.10 and 2.58% respectively, making clear good stability for long term use. Collectively, the developed PDA-BC/Au adsorbent indicates enormous promise for cationic dye removal and efficient utilization of waste biomass resources.
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Qi Zheng,Yuyan Cao,Yang Chen,Wei Zhu,Shenping Zhang,Qing Ye,Chaohui Zhou,Yang Liu,Nengqin Jia
Rapid analysis of xylazine (XYL), a type of drug residue in food of animal origin, is essential for on-site dairy quality monitoring. The conventional analytical methods are encumbered by high costs, complicated sample processing, or cumbersome operational requirements. Here, a gold nanoparticles (Au NPs)/reduced graphene oxide (rGO)-modified screen-printed carbon electrode (SPCE) was prepared via a two-step electrochemical reduction process. The excellent electrical conductivity of Au NPs and the large specific surface area of rGO significantly increase the signal intensity in differential pulse voltammetry (DPV) curves. The XYL was specifically recognized via molecular imprinting technology, utilizing methacrylic acid as a functional monomer and ethylene glycol dimethacrylate as a cross-linker. A portable electrochemical test strip based on this SPCE is capable of rapid detection of XYL in the linear range of 0.1–10 5 μg L −1 with a detection limit as low as 0.02 μg L −1 . This test strip benefits from miniaturization and portability, rendering it suitable for prospective real-time, on-site detection requirements.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Minghan Wan,Bing Xu,Lei Shi,Tian Zhou,Nianben Zheng,Zhiqiang Sun
Nanofluids-based direct absorption solar collectors are promising candidates for medium–high-temperature solar energy harvesting . However, nanofluids’ complicated preparation process and undesirable high-temperature stability have hindered their practical applications. Herein, we propose a facile method for synthesizing gold/carbon quantum dots (Au-CQDs) nanofluids by directly carbonizing the base fluid and spontaneously assembling with Au nanoparticles (AuNPs) triggered by high temperatures. The results indicate that the self-assembled Au-CQDs nanofluids can maintain high stability at 110 °C for 100 h without precipitation and keep excellent photothermal conversion performance under 10 sun irradiation. The concentration and particle size of AuNPs are crucial factors affecting the self-assembly process. By modulating the microscopic morphologies of the self-assembled nanoparticles , the extinction coefficient of the prepared nanofluids is up to 88.7 % at a low loading of 30 ppm. The nanofluids can reach an equilibrium temperature of 50 °C under 1 sun irradiation, 10.4 °C higher than the base fluid due to the enhanced plasmonic effects and stability resulting from the CQDs dotted AuNPs. This work offers a new strategy to fabricate highly stable nanofluids with excellent light absorption properties for efficient solar thermal applications.
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高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Jieyuan Zhang,Ting Wang,Yuxuan Du,Yue Wu,Xinhui Jiang,Hecun Zou,Zhengwei Zhang
Mercury metal can be absorbed into human body through the food chain, and cause considerable harm to human health. Herein, a novel smartphone-enabled ratiometric fluorescence sensing system, which was made up of nitrogen-sulfur co-doped carbon dots (NSCDs) and Au nanoclusters (AuNCs), had developed for rapid detection of mercury ion (Hg 2+ ). The NSCDs/AuNCs displayed dual-emission fluorescence at 406/690 nm. After introducing Hg 2+ , the fluorescence at 690 nm was obviously quenched, while the signal around 406 nm was slightly attenuated, causing a color change from pink-purple to blue. Under the most suitable conditions, the ratiometric fluorescence probe had two linear ranges of 0.05–3.0 and 3.0–7.0 μM with a low limit of detection (LOD) of 18 nM. The approach was utilized to detect Hg 2+ in real water samples. Furthermore, a portable smartphone-assisted sensing platform based on NSCDs/AuNCs provides a simple, affordable, and efficient method for monitoring Hg 2+ in the environments and other fields.
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高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Xiaoping Bai,Cong Tang,Xiaojing Liang,Xue Yu,Qing Shen,Shijun Shao,Guangxiu Liu,Shuqing Dong
Given the increasingly grave situation of bacterial infections and antibiotic resistance, there is an urgent demand for the development of rapid, straightforward, and highly sensitive techniques for bacterial detection and imaging. Here, a highly sensitive and label-free PEC biosensor was proposed for Gram-positive bacteria detection based on the vancomycin (Van) functionalized ITO/BiVO 4 /Au photoelectrode. The Gram-positive bacteria can be captured by the specific recognition of Van, which was immobilized AuNPs of the ITO/BiVO 4 /Au photoelectrode surface, thus enabling extreme acceleration of the detection procedure. Van not only acts as a sensitizer to increase the photocurrent but also acts as a probe molecule to specifically capture Gram-positive bacteria, achieving the signal “On-Off” response with minimized background noise. In addition, electrochemical impedance spectroscopy and scanning photoelectrochemical microscopy were used to illustrate the detection mechanism. The low detection limit of the new PEC biosensor was 15 colony forming units per milliliter (CFU/mL), and the detection range was 50–5 × 10 7 CFU/mL. Moreover, the PEC sensor had good stability and reproducibility, which was conducive to promoting further application and providing a foothold for ultrasensitive bacterial detection in food and environment.
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