ACS Applied Materials & Interfaces
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2014
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IF 5.9
Pingsheng Huang,Jinjian Liu,Weiwei Wang,Chen Li,Junhui Zhou,Xue Wang,Liandong Deng,Deling Kong,Jianfeng Liu,Anjie Dong
Enabling nanocarriers to complete the sophisticated journey from the initial injection site to the targeted tumor cells and achieve “spatiotemporally pinpointed” drug release intracellularly is a challenging task in anticancer drug delivery. Herein, versatile shell-cross-linked nanoparticles (SCNPs) were prepared by one-step assembly of triblock zwitterionic copolymers, polycarboxybetaine methacrylate-block-poly(N-(2-(2-pyridyl disulde) ethyl methacrylamide-block-poly(2-(diisopropylamino) ethyl methacrylate) (PCB-b-PDS-b-PDPA, termed as PCSSD), which was well-defined via reversible additive fragment transfer (RAFT) polymerization, followed by functionalization with Arg-Gly-Asp (RGD). Thus, the RGD-PCSSD SCNPs cooperatively combine the ultra pH-sensitive PDPA core for efficient drug loading and pH-responsive drug release, the disulfide-cross-linked PDS shell that prevents premature drug release, the zwitterionic PCB corona to stabilize the SCNPs and prolong its systemic circulation, the RGD ligand for active tumor targeting and receptor-mediated endocytosis. Doxorubicin (DOX) was loaded as a model medicine (termed as RGD-PCSSD/DOX SCNPs). The dual-sensitivity studies showed that the pH-sensitivity of PDPA core could be adjusted by the shell-cross-linking density, accompanied by better control over premature drug release. Furthermore, results obtained by flow cytometry and fluorescence microscopy analysis demonstrated that once the RGD-PCSS10D/DOX SCNPs were internalized into tumor cells via receptor-mediated endocytosis, boost drug release was observed with considerable cytotoxicity in vitro. The results of ex vivo imaging studies further confirmed the successful drug delivery from the injection site to the tumor tissue. In summary, the well-constructed RGD-PCSS10D/DOX SCNPs with cooperative multifunctionality showed great potential as novel nanocarriers for tumor targeted anticancer drug delivery.
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RSC Advances
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2017
·Vol. 7
·期 41
·25694-25701
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IF 3.108
Xiaoju Shi,Guoyue Lv,Xiaodong Sun,Dianbo Cao,Guangyi Wang,Yulei Chang
Recently, several targeted drug delivery systems have shown promising antitumor activity towards different types of cancer. However, targeting the tumor microenvironment, cellular surface and/or only one organelle may not achieve the expected therapeutic effects. Herein, a targeted drug delivery with stepwise targeting and image-guided strategy was obtained for enhancing antitumor therapy and overcoming various problems caused by the random drug distribution of traditional medicine. In this design, a multifunctional nanoplatform, TPGS/PPG2L@IONPs@DOX/TPP–DOX, based on poly(ethylene glycol)-poly(ε-caprolactone)-poly(amidoamine)-lactose acid (mPEG-PCL-G2.0 PAMAM-LA, PPG2L) copolymer and D-α-tocopherol polyethylene glycol 1000 succinate (TPGS), as a co-carrier, incorporates iron oxide nanoparticles (IONPs) as imaging agents and loads a DOX/triphenylphosphonium (TPP)–DOX drug for chemotherapy. In this nanoplatform micelle, the LA as targeting moieties to hepatocarcinoma cells is the first targeting step. After the drug is released from the hybrid micelles, the incorporated DOX and TPP–DOX target delivery to nuclei and mitochondria for the next targeting step. Furthermore, with the help of MRI contrast agents of IONPs, the distribution of hybrid micelles was monitored. As a result, the hybrid micelles demonstrated enhanced antitumor cell activity in both HepG2 and HepG2/DOX cells. The results indicated that the multifunctional hybrid micelles showed great potential in enhancing antitumor activity under image-guided and stepwise targeted chemotherapy.
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INDUSTRIAL CROPS AND PRODUCTS
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2015
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IF 2.837
Li-Hong Tan,Dan Zhang,Gang Wang,Bao Yu,Sheng-Ping Zhao,Jian-Wei Wang,Ling Yao,Wei-Guo Cao
The aim of this study was to compare the flavonoids compositions, content and antioxidant activities of Hawk tea from six botanical origins for screening the most suitable botanical origins. The main flavonoids of Hawk teas from Litsea coreana Levl. var. lanuginose were identified, and six high contents of individual flavonoids (hyperin, isoquercitrin, quercitrin, astragalin, quercetin and kaempferol) were quantitative and comparative analysis. Moreover the potential antioxidant capacity of Hawk tea was measured by using in vitro antioxidant assays. The above experimental data were all used for the principal component analysis. The statistical results showed that the extracts from Machilus chuanchienensis S. Lee and L. coreana Levl. var. lanuginose appeared closely positioned in the positive regions, indicated the higher flavonoids and antioxidant activity compared with other species. Thus, they were temporarily considered as good sources of Hawk tea.
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JOURNAL OF CONTROLLED RELEASE
·
2017
·
IF 7.786
Yingying Chi,Xuelei Yin,Kaoxiang Sun,Shuaishuai Feng,Jinhu Liu,Daquan Chen,Chuanyou Guo,Zimei Wu
This study aimed to develop redox-sensitive and CD44-targeted liposomes to improve chemotherapy of osteosarcoma . Cationic liposomes were prepared and stabilized with a novel detachable polyethylene glycol (PEG 2000 ) conjugated with cholesterol through a bio-reducible disulfide linker (Chol-SS-mPEG). Hyaluronic acid (HA, MW 20–40 kDa), a ligand to CD44, was non-covalently coated on the cationic liposomes. Doxorubicin (DOX) was actively loaded in the liposomes as a model drug. The roles of HA and Chol-SS-mPEG on intracellular drug delivery efficiency, and antitumor efficacy were studied. The structure of Chol-SS-mPEG was confirmed with Fourier-transform infrared and nuclear magnetic resonance ( 1 H NMR). The liposomes, Chol-SS-mPEG/HA-L had a mean diameter of 165 nm, zeta potential − 28.9 mV, and destabilized in reducing or acidic (pH 5–6) conditions. In vitro release of DOX was well-controlled at physiological conditions, but a burst release of 60% was observed in the presence of 10 mM glutathione (GSH), in contrast to non-redox sensitive liposomes (Chol-mPEG/HA-L and Chol-mPEG-L). MTT cell viability assay showed that the dual-functional Chol-SS-mPEG/HA-L with a drug loading of 15.0% (w/w) had significantly higher cytotoxicity to MG63 osteosarcoma cells compared with non-reduction sensitive or non-HA coated liposomes (p < 0.01), consistent with the cellular uptake and intracellular trafficking studies using confocal microscopy and flow cytometry. Furthermore, the HA-coated GSH-responsive liposomes preferentially internalized to MG63 over human liver cells LO2. In rats, liposomes stabilized with either Chol-SS-mPEG or Chol-mPEG, with or without HA, increased the half-life of DOX by > 10-fold. In a MG63 xenograft mouse model, Chol-SS-mPEG/HA-L showed the most effective tumor suppression with minimal uptake by the liver compared with other liposomes. All animals treated with liposomal formulations survived, in contrast to those free-DOX treated. In conclusion, the easily prepared Chol-SS-mPEG/HA-L was demonstrated as an excellent CD44-mediated intracellular delivery system capable of long-circulation and GSH-triggered cytoplasmic drug release. Further translational and multidisciplinary research is required to make it real clinical benefits to cancer patients.
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INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2017
·Vol. 52
·期 5
·1176-1184
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IF 1.64
Jing Li,Wenting Shang,Xu Si,Dandan Bu,Padraig Strappe,Zhongkai Zhou,Chris Blanchard
Summary Corn bran polysaccharides were extracted using an ultrasonic-assisted method with a yield of 33.50% and then carboxymethylated. The occurrence of modification was confirmed using Fourier transform infrared spectral analysis. The surface of polysaccharide particles became loosened following the carboxymethylation. The effect of sodium hydroxide concentration, reaction temperature, and the reaction time on the degree of substitution was investigated, and the relationship among the substitution degree, total phenolic content and antioxidant activity were studied. With sodium hydroxide concentration of 6.0 mol L −1 , keeping the reaction temperature of 60 °C and reaction time of 5.0 h, the modified polysaccharides possessed the highest degree of substitution and antioxidant activity. Cell culture model indicated that the carboxymethylated polysaccharide significantly inhibited the proliferation of A549 and HepG-2 cells, and these antitumour characteristics may be associated with the elevated expression of CASP3 , CASP8 , CASP9 , and p53 , as well as the significantly decreased expression of Bcl-2 and iNOS genes. The excellent antioxidant capacity and strong antitumour activity of these polysaccharides might provide a potential application as a naturally functional ingredient.
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INORGANIC CHEMISTRY
·
2017
·
IF 4.857
Lan Wang,Xiaojiao Kang,Daocheng Pan
Assisted with polyethylenimine, 4.0 L of water-soluble AgInS2 quantum dots (AIS QDs) were successfully synthesized in an electric pressure cooker. As-prepared QDs exhibit yellow emission with a photoluminescence (PL) quantum yield up to 32%. The QDs also show excellent water/buffer stability. The highly luminescent AIS QDs are used to explore their dual-functional behavior: detection of hydrogen peroxide (H2O2)/glucose and cell imaging. The amino-functionalized AIS QDs show high sensitivity and specificity for H2O2 and glucose with detection limits of 0.42 and 0.90 μM, respectively. A linear correlation was established between PL intensity and concentration of H2O2 in the ranges of 0.5–10 μM and 10–300 μM, while the linear ranges were 1–10 μM and 10–1000 μM for detection of glucose. The AIS QDs reveal negligible cytotoxicity on HeLa cells. Furthermore, the luminescence of AIS QDs gives the function of optical imaging.
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PHYSICA SCRIPTA
·
2024
·Vol. 100
·期 1
·015024
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IF 2.6
Tingfei Chen,Yanzhi Huang,Xiaojun Liu,Chunnan Zhu,Chao Liu,Dongyun Zheng
The accurate and rapid detection of catechol which is a class of highly toxic organic phenolic compounds, is of great importance for the protection of environment and human health. In this work, a novel electrochemical sensor for catechol was constructed by modifying multi-walled carbon nanotube paste microelectrode with a cationic surfactant of cetyltrimethylammonium bromide (CTAB) through a simple and controllable adsorption method. Electrochemical experiment results show that the modified electrode has good sensitization effect to the electrochemical response of catechol. The electrochemical response mechanism of the sensor to catechol were investigated through using many analytical methods, including scanning electron microscopy, energy-dispersive spectroscopy, Fourier-transform infrared spectroscopy, Raman spectroscopy, and electrochemical techniques. Under the optimal fabrication and application conditions, the response current of catechol on the sensor exhibits a good linear relationship with its concentration from 1.0 μmol l−1 ∼ 7.0 mmol l−1 with a sensitivity of 1.33 nA/(μmol l−1) and a low detection limit of 15 nmol l−1 (S/N = 3). Applying the sensor in the detection of catechol in tap water samples and urine samples, the results were satisfactory, indicating its good application prospect.
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Discover Oncology
·
2024
·Vol. 15
·期 1
·1-13
·
IF 2.8
Deng Chunmin,Zhang Hao,Song Li
Nanoparticle-based photothermal therapy (PTT) has emerged as a promising approach in tumor treatment due to its high selectivity and low invasiveness. However, the penetration of near-infrared light (NIR) is limited, leading it fails to induce damage to the deep-seated tumor cells within the tumor tissue. Additionally, inefficient uptake of photothermal nanoparticles by tumor cells results in suboptimal outcomes for PTT. In this study, we utilized the adhesive properties of photothermal material, polydopamine (PDA), which can successfully load the photosensitizer indocyanine green (ICG) and chemotherapeutic drug doxorubicin (DOX) to achieve photothermal and chemotherapy synergy treatment (PDA/DOX&ICG), aiming to compensate the defects of single tumor treatment. To extending the blood circulation time of PDA/DOX&ICG nanoparticles, evading clearance by the body immune system and achieving targeted delivery to tumor tissues, a protective envelopment was created using erythrocyte membranes modified with folate acid (FA-EM). After reaching the tumor tissue, the obtained FA-EM@PDA/DOX&ICG nanoparticles can specific bind with folate acid receptors on the surface of tumor cells, which can improve the uptake behavior of FA-EM@PDA/DOX&ICG nanoparticles by tumor cells, and leading to the release of loaded DOX and ICG in response to the unique tumor microenvironment. ICG, as a typical photosensitizer, significantly enhances the photothermal conversion performance of FA-EM@PDA/DOX&ICG nanoparticles, thus inducing tumor cells damage. In vitro and in vivo experimental results demonstrated that the coordinated NIR treatment with FA-EM@PDA/DOX&ICG not only effectively inhibits tumor growth, but also exhibits superior biocompatibility, effectively mitigating DOX-induced tissue damage.
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Materials Today Bio
·
2025
·
IF 10.2
Dan Yin,Xuan Wu,Xu Chen,Jian-Li Chen,Xinyue Xia,Jianfang Wang,Xiuping Chen,Xiao-Ming Zhu
Encouraged by the clinical success of proteasome inhibitors treating hematological malignancy, continuous efforts are being made to improve their efficacy and expand their applications to solid tumor therapy. In this study, liposomes were used to encapsulate the proteasome inhibitor carfilzomib (CFZ) and calcium peroxide (CaO 2 ) nanoparticles for effective combination therapy targeting the interplay between calcium overload and oxidative stress. Low-dose CaO 2 synergistically enhances the anticancer effect of CFZ in the human glioblastoma U-87 MG cells. The reactive oxygen species (ROS) generation and glutathione depletion by low-dose CaO 2 complement CFZ-induced ubiquitinated protein accumulation further triggering endoplasmic reticulum (ER) stress leading to calcium overload and mitochondrial dysfunction. The liposome-based codelivery system is capable of transporting CFZ and CaO 2 simultaneously to the tumor, and results in a superior antitumor effect in U-87 MG tumor-bearing mice compared with monotherapy. Taken together, CaO 2 holds great potential to sensitize proteasome inhibitors in the treatment of solid tumors, and this work also presents a new combination therapy strategy targeting the crosstalk between proteasome inhibitors and oxidative stress for future cancer therapy.
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DIAMOND AND RELATED MATERIALS
·
2025
·
IF 5.1
Chenxi Wu,Mohammad Jahidul Alam,Shengzhou Qiu,Sima Akter,Shaoli Hong,Kazi N. Islam,Huihong Liu,Sakil Mahmud
Hydrochlorothiazide (HCT), a widely used thiazide diuretic, faces detection challenges due to low sensitivity, interference, and complex analysis methods. This study explores an innovative electrochemical sensor based on cobalt oxide-modified screen-printed carbon electrodes (CoO/SPCE) to enhance HCT detection. CoO was electrodeposited onto SPCE, optimizing parameters like the CoO concentration and electrodeposition time through cyclic voltammetry. Microscopic analysis confirmed smoother CoO/SPCE surfaces, indicative of improved structural integrity. The modified electrode demonstrated an oxidation peak at 0.242 V with a current of 5.8 μA for HCT, outperforming unmodified electrodes. Electrochemical impedance spectroscopy revealed a 495-fold decrease in charge transfer resistance after modification, underscoring enhanced electron transfer. The sensor exhibited a low limit of detection, minimal relative standard deviation for signal stability (RSD = 3.38 %), and consistent multi-electrode performance (RSD = 3.32 %). The linear relationship between peak current and HCT concentration, I = 0.01941c + 0.4319 (R 2 = 0.996), confirms the reliability of the method. This CoO/SPCE sensor offers simplicity and rapid detection, marking a significant advancement for in-field HCT analysis.
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JOURNAL OF FLUORESCENCE
·
2024
·
IF 2.6
Chen Xian,Zhang Menghan,Zheng Lang,Deng Xiaoqin,Chen Qiang,Han Wendi,Huang Zhengjun,Weng Shaohuang
The establishment of a convenient and efficient testing method is crucial and needed for the monitoring of diosmin. In this study, nitrogen doped carbon dots (N-CDs) with the particle size distribution of 2.5–5.7 nm and the average diameter of 4.1 nm were successfully synthesized using a simple strategy. N-CDs exhibited excellent and stable fluorescence performance with the quantum yield of 22.33%. Correspondingly, a fluorescence analytical method was developed for diosmin determination using N-CDs as probe. UV-vis absorbance spectroscopy and the evaluation of internal filtering parameters verified that the mechanism causing the quenching of N-CDs was an internal filtering effect (IFE). The concentration of diosmin can be directly evaluated based on the quenched fluorescence intensities. After optimizing experimental conditions, it was found that the fluorescence quenching efficiency ((F 0 -F)/F 0 ) of N-CDs exhibited a good linear relationship with the concentration of diosmin (C Diosmin ) in the range of 3.0–50 µg/mL. The limit of detection (LOD) was 0.86 µg/mL based on 3σ/slope ( n = 13). This method was successfully applied to accurately determine the content of diosmin in diosmin tablets and human plasma samples with good reproducibility. It stands out for its simplicity, speed, and acceptable determination performance.
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ACS Nano
·
2025
·
IF 16
Jia Liu,Liuting Zheng,Xinyue Li,Wei Tang,Manyu Guo,Yuxing Wang,Xiaoqi Tan,Jiajia Chang,Huiyue Zhao,Dongsheng Zhu,Yu-qiang Ma,Da Huo
Understanding the interaction between nanomaterials and cellular structures is crucial for nanoparticle applications in biomedicine. We have identified a subtype of stress granules, called nanomaterial-provoked stress granules (NSGs), induced by gold nanorods (AuNRs). These NSGs differ from traditional SGs in their physical properties and biological functions. Uptake of AuNRs causes reactive oxygen species accumulation and protein misfolding in the cell, leading to NSG formation. Physically, NSGs have a gel-like core and a liquid-like shell, influenced positively by HSP70 and negatively by HSP90 and the ubiquitin-proteasome system. AuNRs promote NSG assembly by interacting with G3BP1, reducing the energy needed for liquid–liquid phase separation (LLPS). NSGs impact cellular functions by affecting mRNA surveillance and activating Adenosine 5′-monophosphate (AMP)-activated protein kinase signaling, crucial for a cellular stress response. Our study highlights the role of LLPS in nanomaterial metabolism and suggests NSGs as potential targets for drug delivery strategies, advancing the field of nanomedicine.
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