Plants-Basel
·
2022
·Vol. 11
·期 19
·2487
·
IF 4.658
Yifei Zhang,Yishan Sun,Weiqing Li,Jiayu Li,Rongqiong Xu,Jiarui Du,Zesong Li,Guibin Li,Kejun Yang
To explore the effects of iminodisuccinic acid (a chelating agent) on maize (Zea maysL.) seed germination under lead (Pb) stress, we comparatively analyzed the effects of applying different concentrations of iminodisuccinic acid (0, 5, 20, and 100 mmol·dm−3) and combined an addition of exogenous substances regulating reactive oxygen species production on maize seed germination, seedling growth, H2O2content, NADPH oxidase activity, and antioxidant enzyme activities under Pb-stressed and Pb-free conditions. Iminodisuccinic acid (100 mmol·dm−3) significantly delayed seed germination under normal germination conditions and alleviated the inhibitory effects of Pb stress (20 mmol·dm−3) on seed germination. Under normal conditions (without Pb stress), the iminodisuccinic acid-induced inhibition of seed germination was enhanced by treatment with dimethylthiourea (a specific scavenger of reactive oxygen species) or diphenyleneiodonium chloride (a specific inhibitor of NADPH oxidase), but diminished by treatment with H2O2, CaCl2, diethyldithiocarbamic acid (a specific inhibitor of superoxide dismutase), or aminotriazole (a specific inhibitor of catalase). Under Pb stress, iminodisuccinic acid partially eliminated the excessive H2O2accumulation, improved superoxide dismutase and catalase activity, and weakened the high NADPH oxidase activity. In addition, Ca2+chelation may be essential for maintaining the reactive oxygen species’ balance and improving seed germination and seedling growth by iminodisuccinic acid supplementation in maize under Pb stress. The proposed iminodisuccinic acid supplementation-based method improved maize seed germination in Pb-polluted soil.Keywords:maize;chelator;lead stress;ROS;hydrogen peroxide;NADPH oxidase;seed germination
✓ 已验证
DOI
PubMed
高影响因子
Analytical Methods
·
2017
·Vol. 9
·期 47
·6689-6697
·
IF 1.9
Danyu He,Shehong Li,Pu Zhang,Hongxia Luo
A graphene platform electrode (GPE) was prepared directly by transferring chemical vapor deposited (CVD) graphene films from Cu foils to polyethylene terephthalate substrates with the aid of polymethyl-methacrylate, a distinct advantage over previously developed methods. L-Cysteine (L-Cys) was polymerized on the GPE by using a one-step electrodeposition technique to form a poly-L-Cys/GPE. As a negatively charged polymer, the poly-L-Cys could adsorb positively charged dopamine (DA) and repel negatively charged ascorbic acid (AA) under optimum conditions. As a result, a novel electrochemical sensor for the simultaneous determination of DA and AA was developed. The electrochemical behaviors of DA and AA were investigated by cyclic voltammetry. Differential pulse voltammetry was used for determining DA and AA in their mixture, where the separation between oxidation peak potentials of DA and AA was about 163 mV. The broad linear responses to DA and AA were determined by chronoamperometry, which were 0.2 to 423 μM and 1 to 580 μM with detection limits (S/N = 3) of 0.0531 μM and 0.2031 μM, respectively. In addition, the as-obtained sensor delivered good stability as well as high resistance to interference, and was successfully utilized in determining real samples. Consequently, the poly-L-Cys/GPE could be considered as a new promising DA and AA sensor, strongly indicating that CVD graphene could be used successfully as a material in electrochemistry.
✓ 已验证
DOI
高影响因子
Chemistry Africa-A Journal of the Tunisian Chemical Society
·
2023
·Vol. 6
·期 4
·1799-1810
·
IF 2.6
Gogoi Pinku,Kumar Amit,Das Aparoop,Baishya Gakul
The present study was aimed to investigate the role of Dillenia indica L. bark extracts and their kinetics of inhibition against the tyrosinase enzyme. The kinetics study of various concentrations of the extracts was executed by spectrophotometric assay. Out of four extracts, only hydro-ethanolic extract of D. indica barks (HEEBDI) showed the maximum radical scavenging capacity (36.73 ± 1.65 μg/ml) compared to ascorbic acid. Additionally, HEEBDI scavenging capacity and tyrosinase inhibitory properties showed a significant correlation. In HEEBDI, fifteen marker compounds were identified by LC-QTOF-MS method. The tyrosinase inhibitory activity of HEEBDI (IC 50 19.32 ± 0.57 μg/ml) showed a significant value compared to kojic acid (IC 50 22.08 ± 0.19 μg/ml). The inhibitory mechanism was confirmed in a mixed manner by enzyme kinetics. With minimal conformational energy, betulinic acid and kaempferol bind to the tyrosinase enzyme (PDB ID: 2y9x) as proved by their respective docking score and binding interaction.
✓ 已验证
DOI
高影响因子
Journal of Essential Oil Bearing Plants
·
2023
·
IF 2.4
Boyang Zhang,Hai Xin,Haiyao Shang,Wei Zhang,Xuyang Hei
As a natural medication and food plant resource, Liquidambar formosana Hance (L.) leaves are growing popularity in the fields of medicine and health care. The essential oil of L. leaves was extracted by continuous flow microreactor, and the extraction rate is 53.2%. 26 compounds from essential oil were identified by gas chromatography/mass spectrometry (GC/MS). The main components of these compounds are α-pinene, β-pinene and limonene. Ten of them are reported for the first time. The antibacterial activity of essential oil was investigated by the inhibition zone method, minimum inhibitory concentration and inhibition growth curve experiment. The results show that the essential oil had inhibitory effects on both Escherichia coli (E. coli) and Bacillus subtilis (B.subtilis), and the minimum inhibitory concentrations are 1.0 μg/mL and 1.2 μg/mL. The inhibition effect on gram-negative bacteria E.coli is stronger. The results of this study show that the essential oil contains rich medicinal value components and have great potential for exploitation. This research provided a scientific foundation for understanding essential oil while also providing a theoretical foundation for their full and rational utilization. Simultaneously, research into the antibacterial properties of essential oil provides a scientific basis for the further development of the anti-bacterial agents that exists in plants.
✓ 已验证
DOI
高影响因子
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
·
2014
·Vol. 20
·期 1
·109-121
·
IF 3.164
Zhang Zhongyu,Bi Caifeng,Fan Yuhua,Zhang Nan,Deshmukh Rahul,Yan Xingchen,Lv Xiuwen,Zhang Pengfei,Zhang Xia,Dou Q. Ping
Abstract Ubiquitin–proteasome system (UPS) plays a crucial role in many cellular processes such as cell cycle, proliferation and apoptosis. Aberrant activation of UPS may result in cellular transformation or other altered pathological conditions. Previous studies have shown that metal-based complexes could inhibit proteasome activity and induce apoptosis in certain human cancer cells. In the current study, we report that the cadmium and copper complexes with heterocycle-ornithine Schiff base are potent inhibitors of proteasomal chymotrypsin-like (CT-like) activity, leading to induction of apoptosis in cancer cells. Two novel copper-containing complexes and two novel cadmium-containing complexes with different heterocycle-ornithine Schiff base structures as ligands were synthesized and characterized. We found that complexes Cu1, Cd1 and Cd2 show proteasome-inhibitory activities in human breast cancer MDA-MB-231 and human prostate cancer LNCaP cells, resulting in the accumulation of p27, a natural proteasome substrate and other ubiquitinated proteins, followed by the induction of apoptosis. Our results suggest that metal complexes with heterocycle-ornithine Schiff base have proteasome-inhibitory capabilities and have the potential to be developed into novel anticancer drugs. Graphical Abstract
✓ 已验证
DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 9
·2688
·
IF 4.927
Tao Chen,Fei-Fan Shuang,Qing-Yue Fu,Yu-Xiong Ju,Chen-Man Zong,Wei-Guo Zhao,Dong-Yang Zhang,Xiao-Hui Yao,Fu-Liang Cao
Mulberry (Morus albaL.) fruit is a fruit with nutritional and medicinal value. It is widely cultivated in different regions of China, which may result in differences in its chemical composition. In this research, 25 mulberry fruit samples from six provinces in China were investigated. The contents of anthocyanins were evaluated by high-performance liquid chromatography (HPLC). The contents of two main anthocyanins, cyanidin-3-O-glucoside (C3G) and cyanidin-3-O-rutinoside (C3R), ranged from 0.656 ± 0.006 mg/g to 4.367 ± 0.243 mg/g and from 0.226 ± 0.007 mg/g to 1.649 ± 0.013 mg/g, respectively. Additionally, the contents of total phenolic, total flavonoid, vitamin C, titratable acids, reducing sugars and antioxidant capacity (FRAP, DPPH, scavenging and hydroxyl radical scavenging activity) were also assessed. The results and principal component analysis showed that the Zhongsang 5801 variety from Sichuan, Dechang had the greatest health value with the highest active compound contents. Based on our analysis, the variety from Sichuan, Dechang is a high-quality plant source for mulberry fruit cultivation. This research provides a basis for the rational development and utilization of mulberry fruit resources in China.Keywords:mulberry fruit;varieties and regions;anthocyanin;chemical composition;antioxidant activity
✓ 已验证
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2016
·
IF 3.902
Yang Hu,Shanshan Ma,Zhuohong Yang,Wuyi Zhou,Zhengshan Du,Jian Huang,Huan Yi,Chaoyang Wang
In this study, we develop a facile one-pot approach to the fabrication of poly(L-lactic acid) (PLLA) microsphere-incorporated calcium alginate (ALG-Ca)/hydroxyapatite (HAp) porous scaffolds based on HAp nanoparticle-stabilized oil-in-water Pickering emulsion templates, which contain alginate in the aqueous phase and PLLA in the oil phase. The emulsion aqueous phase is solidified by in situ gelation of alginate with Ca 2+ released from HAp by decreasing pH with slow hydrolysis of d -gluconic acid δ-lactone (GDL) to produce emulsion droplet-incorporated gels, followed by freeze-drying to form porous scaffolds containing microspheres. The pore structure of porous scaffolds can be adjusted by varying the HAp or GDL concentration. The compressive tests show that the increase of HAp or GDL concentration is beneficial to improve the compressive property of porous scaffolds, while the excessive HAp can lead to the decrease in compressive property. Moreover, the swelling behavior studies display that the swelling ratios of porous scaffolds reduce with increasing HAp or GDL concentration. Furthermore, hydrophobic drug ibuprofen (IBU) and hydrophilic drug bovine serum albumin (BSA) are loaded into the microspheres and scaffold matrix, respectively. In vitro drug release results indicate that BSA has a rapid release while IBU has a sustained release in the dual drug-loaded scaffolds. In vitro cell culture experiments verify that mouse bone mesenchymal stem cells can proliferate on the porous scaffolds well, indicating the good biocompatibility of porous scaffolds. All these results demonstrate that the PLLA microsphere-incorporated ALG-Ca/HAp porous scaffolds have a promising potential for tissue engineering and drug delivery applications.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2018
·
IF 3.115
Wenhao Xu,Meiqun Hu,Qihong Zhang,Jingbo Yu,Weike Su
Ethnopharmacological relevance Cassia occidentalis Linn. is a traditional ayruvedic edible shrub containing anthraquinones (AQs) as the principle active constituents. In folk medicine, it has a variety of uses including treatment of whooping cough (‘pertussis’) and inflammatory diseases . Despite these applications, limited data are available to validate the effects of C. occidentalis AQs on airways inflammation in asthma. Aim of the study To explore the anti-inflammatory potential of AQs extracted from C. occidentalis using an in vivo model of ovalbumin (OVA)-induced asthma. Materials and methods Extraction and optimization of AQs from C. occidentalis was performed by mechanochemistry. Allergic asthma in BALB/c mice was sensitized and challenged by OVA, and the effects of AQs investigated in a mouse model. OVA-specific IgE concentrations in serum, and Th1/Th2 cytokine (IL-4, IL-5, IL-13 and IFN- γ ) concentrations, inflammatory cell counts and classification in bronchoalveolar lavage fluid (BALF) were determined. Histopathological evaluation of lung tissue was performed using hematoxylin and eosin (H&E), and periodic acid-schiff (PAS) staining. Th1/Th2 cytokine mRNA expression was analyzed using the 2 -ΔΔCt method. Results Treatment with AQs decreased inflammatory cell counts and production of Th2 cytokines (IL-4, IL-5 and IL-13) in BALF, and OVA-specific IgE in serum. In contrast,Th1 cytokine IFN- γ production in BALF was promoted. AQs also decreased mRNA expression of Th1/Th2 cytokine in lung tissue. Histological studies demonstrated that AQs substantially inhibited OVA-induced cellular infiltration, mucus hypersecretion and goblet cell hyperplasia in the lung. Conclusions These findings demonstrated the inhibitory effects of AQs, derived from C. occidentalis, on OVA-induced allergic asthma in mice. The results suggest a promising ethnopharmacological use for AQs in patients with asthma.
✓ 已验证
DOI
PubMed
高影响因子
Journal of Pain Research
·
2022
·
IF 2.832
Tiansheng Liu,Bin Su
Introduction This research was to evaluate the beneficial effects of Styphnolobium japonicum (L.) Schott flower extract (SJF) on the adjuvant-induced arthritis rat model.Methods Arthritis was evoked by injection of complete Freund’s adjuvant (CFA) in the hind paw. SJF (150 or 300 mg/kg/day) or Celecoxib (5 mg/kg/day) were administered intragastrically from the 0th day to the 28th day. The arthritis symptoms (paw edema, arthritic scores, mechanical hyperalgesia, and thermal hyperalgesia), inflammation biomarkers (RT and CRP), related enzymes (MMP1 and MMP13), oxidative stress markers (CAT, SOD, GPx, and MDA), and inflammatory cytokines (IL-6, IFN-γ, TNF-α, and IL-1β) of SJF-treated CFA rats were evaluated.Results CFA rats exhibited severe arthritis symptoms, increased oxidative stress, and inflammatory cytokines. Interestingly, SJF treatment relieving arthritis symptoms and restored body weight gain compared with those in the CFA group. SJF treatment decreased the levels of CRP, RF, MMP1, and MMP13 in the CFA group. Besides, SJF treatment increased the activities of antioxidant enzymes and decreased the MDA content and inflammatory cytokines compared with those in the CFA group. Moreover, SJF could increase the mRNA expression of GPx-1 and CAT and inhibit the mRNA expression of IL-6 and TNF-α in the ankle tissue of CFA rats.
✓ 已验证
DOI
PubMed
高影响因子
Applied Materials Today
·
2016
·
IF 0
Huishang Yang,Guanglin Zhu,Yicheng Huang,Xuetao Shi,Yingjun Wang
This study reports a novel method in which polycaprolactone(PCL)/polyaniline(PANi) nanofibre mesh is used to promote the differentiation of pheochromocytoma (PC12-L) cells into neuron-like cells. Stainless steel wire mesh (304) was used as a collector in the rapid fabrication of alignment-patterned PCL/PANi nanofibre mesh. Successful incorporation of PANi improved the electrical conductivity of the nanofibre mesh. Culturing low-differentiated rat pheochromocytoma (PC12-L) cells on the electrically conductive nanofibre mesh significantly promoted the differentiation of PC12-L cells into neuron-like cells by the synergetic effect of the alignment patterns and the electrical conductivity of PANi. The electrically conductive nanofibre mesh described in this study may provide a promising platform for the study of nerve regeneration.
✓ 已验证
DOI
Nano Research
·
2016
·Vol. 9
·期 12
·3683-3694
·
IF 8.893
Deng Jun,Zheng Honghao,Zheng Xiaowen,Yao Mengyun,Li Zheng,Gao Changyou
Chirality is one of the most distinctive biochemical signatures of life, and plays crucial roles in maintaining normal functions of living cells or organisms. Pioneering work from another group has demonstrated the dependency of cell differentiation on the chirality of nano-coated substrates, but the effect of the chiral surface of nanoparticles on stem cell fates has not been investigated. In this study, the influence of molecular chiral poly(acryloyl-L(D)-valine) (L(D)- PAV)-anchored gold nanoparticles (L(D)-PAV-AuNPs) on the differentiation of mesenchymal stem cells (MSCs) was investigated. Though osteogenic differentiation of MSCs was not affected by D-PAV-AuNPs, it was significantly promoted by L-PAV-AuNPs in terms of calcium deposition, alkaline phosphatase (ALP) activity, and expression of collagen type I and osteocalcin (OCN) at both mRNA and protein levels. L-PAV-AuNPs could activate the P38 mitogen-activated protein kinase (MAPK) pathway, and may exert mechanical stress on MSCs because of high amounts of internalization. These results provide new insights on surface chirality at the nanoscale as a direct regulator to guide the differentiation of MSCs, and the use of these nanomaterials for strategic regenerative medicine.
✓ 已验证
DOI
高影响因子
ELECTRONIC JOURNAL OF BIOTECHNOLOGY
·
2020
·
IF 2.894
Longxing Wang,Wenjun Zhu,Zhen Gao,Hua Zhou,Fei Cao,Min Jiang,Yan Li,Honghua Jia,Ping Wei
Background L- tert -Leucine has been widely used in pharmaceutical, chemical, and other industries as a vital chiral intermediate. Compared with chemical methods, enzymatic methods to produce L- tert -leucine have unparalleled advantages. Previously, we found a novel leucine dehydrogenase from the halophilic thermophile Laceyella sacchari ( Ls LeuDH) that showed good thermostability and great potential for the synthesis of L- tert -leucine in the preliminary study. Hence, we manage to use the Ls LeuDH coupling with a formate dehydrogenase from Candida boidinii ( Cb FDH) in the biosynthesis of L- tert -leucine through reductive amination in the present study. Result The double-plasmid recombinant strain exhibited higher conversion than the single-plasmid recombinant strain when resting cells cultivated in shake flask for 22 h were used. Under the optimized conditions, the double-plasmid recombinant E. coli BL21 (pETDute-FDH-LDH, pACYCDute-FDH) transformed 1 mol·L -1 trimethylpyruvate (TMP) completely into L- tert -leucine with greater than 99.9% ee within 8 h. Conclusions The Ls LeuDH showed great ability to biosynthesize L- tert -leucine. In addition, it provided a new option for the biosynthesis of L- tert -leucine. How to cite Wang L, Zhu W, Gao Z, et al. Biosynthetic L- tert -leucine using Escherichia coli co-expressing a novel NADH-dependent leucine dehydrogenase and a formate dehydrogenase. Electron J Biotechnol 2020;47. https://doi.org/10.1016/j.ejbt.2020.07.001
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）