AICHE JOURNAL
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2024
·
IF 3.5
Xin Zhao,Ruiqi Fang,Fengliang Wang,Yingwei Li
Here, we report the fabrication of atomic-metal catalysts for the facile cascaded transformation of lignin β-O-4 dimeric model compound into quinoline. A series of dual-metal single-atom (SA) moieties (MaC 4 and Mb 2 N 6 , where Ma and Mb represent two different metal SAs, C and N represent carbon and nitrogen atoms, respectively) are integrated on N-doped carbons with designed compositions and coordination configurations. Characterizations indicate the homogeneous distribution of uniform and modifiable SA sites, over which the electron distribution is significantly polarized due to synergistic interactions. The distinctive physicochemical properties of catalysts afford attractive performances in the cascaded synthesis of quinoline from lignin β-O-4 dimeric model compound through cascaded transformation. In a continuous flow instrument, CuC 4 /Co 2 N 6 @NC furnishes 93.7% yield of 2-phenylquinoline with a space-time yield of 75.7 g g cat h −1 . Control experiments and density functional theory calculations reveal the synergistic interactions between CuC 4 and Co 2 N 6 endow the catalyst with high efficiency in cascaded transformation.
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Journal of Water Process Engineering
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2024
·
IF 6.3
Yulin Yuan,Qiongfang Wang,Xin Zhang,Lei Dong,Cheng Peng,Min Zhang,Pinhua Rao,Naiyun Gao,Jing Deng
The residues of sulfonamide antibiotics in the environment would pose a threat to aquatic ecosystem and human health. In this study, β-FeOOH@ZnO was synthesized by using a hydrothermal method to activate peroxymonosulfate (PMS) for the degradation of the typical sulfonamide antibiotic sulfadimethoxine (SDM), in which 98.89 % of SDM was removed in 60 min with activating 0.5 mM PMS. Moreover, the influence of catalyst dosage, PMS dosage, solution pH and anions on the degradation efficiency of SDM was investigated in the system. The results indicated that the material exhibited good applicability and β-FeOOH@ZnO performed well in a wide pH range (5–11). 1 O 2 was the major reactive oxygen species (ROS) rather than ˙ OH , S O 4 ˙ − and O 2 ˙ − through free radical quenching experiments and electron paramagnetic resonance experiments (EPR). Furthermore, the mechanism was found that ZnO could enhance the electron transfer pathway of β-FeOOH, which accelerated the degradation of SDM. Possible intermediate products and degradation pathways were proposed through LC-MS analysis. In summary, this study offered a new strategy for the efficient degradation of sulfonamide antibiotics in water treatment.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Dong Cai,Xinmiao Wang,Qian Wang,Peiyong Tong,Weiyuan Niu,Xu Guo,Jinghan Yu,Xuan Chen,Xiaoyang Liu,Dayong Zhou,Fawen Yin
The freeze-drying approach was used to create inclusion complexes utilizing alkyl gallates and β-cyclodextrin, namely dodecyl gallate, octyl gallate, butyl gallate, and ethyl gallate, which are exemplary examples of phenolic esters. The everted-rat-gut-sac model demonstrated that the inclusion complexes released alkyl gallates, which were subsequently hydrolyzed to generate free gallic acid, as evidenced by HPLC-UV analysis. Both gallic acid and short-chain alkyl gallates were capable of permeating the small intestinal membrane. The transport rate of gallic acid (or alkyl gallates) exhibited an initial rise followed by a drop when the carbon-chain lengths varied. The inclusion complex groups exhibited a superior sustained-release effect compared to the comparable alkyl gallates groups, thus possibly leading to higher bioavailability and stronger bioactivity. Moreover, altering the length of the carbon chain will allow for the effortless achievement of regulated release of phenolic compounds and short-chain phenolic esters from such β-cyclodextrin inclusion complexes.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
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IF 5.7
Chengqiang Li,Zhuoning Cao,Hong Jiang,Francesco Secundo,Xiangzhao Mao
β-N-Acetylhexosaminidases have attracted much attention in the enzymatic synthesis of lacto-N-triose II (LNT2) as a backbone precursor of human milk oligosaccharides (HMOs). In this study, a novel glycoside hydrolase (GH) 20 family β-N-acetylhexosaminidase, FlaNag2353, from Flavobacterium algicola was biochemically characterized and applied to synthesize LNT2. FlaNag2353 displayed optimal activity to p-nitrophenyl N-acetyl-β-d-glucosaminide (pNP-GlcNAc) at 40 °C and pH 8.0. In addition to its excellent hydrolysis activity toward pNP-GlcNAc and chitooligosaccharides, FlaNag2353 showed trans-glycosylation activity. Under conditions of pH 9.0 and 55 °C for 2 h and utilizing 200 mM lactose and 10 mM pNP-GlcNAc, FlaNag2353 synthesized LNT2 with a conversion ratio of 4.15% calculated from pNP-GlcNAc. Moreover, when applied to LNT2 synthesis with 10 mM pNP-GlcNAc and 9.7% (w/v) industrial waste whey powder, FlaNag2353 achieved a conversion ratio of 2.39%. This study has significant implications for broadening the applications of GH20 β-N-acetylhexosaminidases and promoting the high-value utilization of whey powder.
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NEW JOURNAL OF CHEMISTRY
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2017
·Vol. 41
·期 24
·15187-15194
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IF 3.269
Jing Wu,Yue Xing,Huan Wang,Hongjing Liu,Mei Yang,Jingli Yuan
Although molecular luminescent probes capable of detecting target analytes in an organelle of interest are especially appreciated, lanthanide complex-based organelle-targetable luminescent probes have rarely been developed. Herein, by using an anthryl-substituted terpyridine derivative, 4′-(9-anthryl)-2,2′:6′,2′′-terpyridine (ATPY), as a singlet oxygen (1O2)-recognition ligand, and a diphenyl-containing β-diketone coligand, 6-(1′,1′′-diphenyl-4′-yl)-1,1,1,2,2,3,3-heptafluoro-4,6-hexanedione (DHH), a new β-diketonate–Eu3+ complex-based luminescent probe, Eu(DHH)3(ATPY), has been designed and synthesized for the time-gated luminescence imaging detection of 1O2 in the mitochondria of living cells. The probe showed only weak luminescence due to the quenching effect of the 9-anthryl group. After the specific reaction of the 9-anthryl group with 1O2, the corresponding endoperoxide derivative that exhibited great quantum yield (Φ = 53%), Eu(DHH)3(EP-ATPY), was formed, accompanied by a remarkable luminescence enhancement. The luminescence response behaviors of Eu(DHH)3(ATPY) to 1O2 were investigated using two different 1O2 reaction systems in aqueous media. In addition, the time-gated luminescence imaging study has demonstrated the applicability of the probe for monitoring the 1O2 generation in 5-aminolevulinic acid (a drug for photodynamic therapy) loaded HepG2 cells with high sensitivity and selectivity. Furthermore, by coloading the probe and a mitochondrial indicator, Mito-Tracker Green, into HepG2 cells, the mitochondria-targetable feature of the probe has also been confirmed by confocal luminescence imaging measurements.
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Journal of Materials Chemistry B
·
2024
·Vol. 12
·期 45
·11696-11707
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IF 6.1
Songyan Guo,Youbei Qiao,Chaoli Wang,Yuming Zhang,Tiehong Yang,Hong Wu
Nanoparticles (NPs) derived from branched copolymers of poly (β-L-malic acid) (PMLA) have been extensively investigated for drug delivery due to their high density of pendant carboxyl groups. This abundant functional group availability enhances their potential as effective drug delivery systems; however, the strong negative charge of PMLA poses a challenge in its uptake by cancer cells due to electrostatic repulsion. In this study, we developed novel enzyme- and pH-sensitive nanoparticles (EP-NPs) based on PMLA, demonstrating tumor-specific behavior and selective activation within tumor tissues. To enhance the cellular internalization of the nanoparticles, we incorporated transactivator of transcription (TAT). In summary, long-chain polyethylene glycol (PEG) was conjugated to PMLA to confer specificity to the TAT peptide. This was achieved using a tetrapeptide linker: alanine–alanine–asparagine–leucine (AANL), which serves as a substrate for legumain. Legumain is a highly conserved cysteine protease primarily found in lysosomes and blood vessels, initially discovered in legumes. It is markedly overexpressed in numerous solid tumors, as well as in endothelial cells and tumor-associated macrophages. The release of doxorubicin in tumor cells was sustained due to the low pH (5.0–5.5) and degradation of PMLA. The PEG modification optimized the particle size and shielded the nanoparticles from plasma proteins and detection by the reticuloendothelial system, thereby prolonging their long circulation time. Once the nanoparticles reached the tumor microenvironment, the AANL was cleaved by legumain, exposing the TAT peptide on the surface, which enhances cellular internalization. Both in vitro and in vivo efficacy studies demonstrated that these EP-NPs significantly inhibited tumor growth while exhibiting negligible systemic toxicity, thereby suggesting that the developed enzyme/pH-sensitive PMLA-based nanoparticle holds great promise as an anti-tumor drug delivery system.
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Cell Reports
·
2025
·
IF 6.9
Zu-Guo Zheng,Yi-Ping Zhang,Xiao-Yu Zhang,Meng-Yao Qin,Yin-Yue Xu,He Wu,Run-Qing Liu,Qiu-Yi Wu,Ming-Su Wang,Chong Zhang,Yue-Qin Zheng,Jian-Ye Dai,Ping Li,Hua Yang
Sterols target sterol-sensing domain (SSD) proteins to lower cholesterol and circulating and hepatic triglyceride levels, but the mechanism remains unclear. In this study, we identify acyl-coenzyme A (CoA) synthetase long-chain family member 1 (ACSL1) as a direct target of ergosterol (ES). The C-terminal domain of ACSL1 undergoes conformational changes from closed to open, and ES may target the drug-binding pocket in the acetyl-CoA synthetase-like domain 1 (ASLD1) of ACSL1 to stabilize the closed conformation and maintain its activity. Moreover, ES is mainly enriched in the mitochondria and promotes fatty acid β-oxidation through ACSL1 allosteric activation. Structure-activity relationship analysis reveals how different structural sterols interact with the sterol-sensing domain-containing protein (SCAP) and ACSL1, explaining their regulatory effects on lipid metabolism. Moreover, our findings reveal that the combination of SCAP inhibitor 25-hydroxycholesterol (25-HC) and ES has a stronger lipid-lowering effect than alone.
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MATERIALS RESEARCH INNOVATIONS
·
2024
·
IF 0
Mengyuan Zhao,Yunhang Liu,Jiale Han,Chaopu Yang,Hongyuan Zhao
In this work, we prepared a sensing platform by utilising the nanocomposite of Vulcan XC-72 R carbon nanoparticles and β-cyclodextrin (β-CD/VXC-72 R) to decorate glassy carbon electrode for detecting MP sensitively. For the β-CD/VXC-72 R composite, VXC-72 R possesses high electroactive area and exceptional electrical conductivity, which is conducive to the generation of interwoven carbon conductive structures. β-CD can effectually facilitate the homogeneous dispersion of VXC-72 R and also enhance the accumulate of MP at the sensing interface. Furthermore, the presence of VXC-72 R compensates for the inherent inferior conductivity of β-CD. The collaborative interaction of VXC-72 R and β-CD enabled the prepared sensor to obtain excellent MP detection properties with a low limit of detection (LOD) of 6.7 nM in MP concentration range of 0.1–10 μM. In addition, the β-CD/VXC-72 R modified glassy carbon electrode (GCE) displayed satisfactory repeatability and reproducibility.
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2024
·
IF 6
Yanpeng Zhang,Qiudie Liu,Jiahua Wang,Wei Xu,Guozhen Wang,Zhixiong Hu,Chun Hu
The purpose of this study was to fabricate the binary complexes stabilized emulsion delivery systems for β-carotene using sodium caseinate (NaCas) and high methoxy pectin (HMP), low methoxy pectin (LMP) and ι-carrageenan (CA), and to study the stability and digestibility of the complex emulsion systems. Compared to NaCas emulsion, the complex emulsion systems had higher particle size and the absolute value of ζ-potential, and exhibited better physical stability and better protection of β-carotene from thermal degradation. It was demonstrated that the increase in viscosity of the complex emulsions and the improvement of the interfacial viscoelasticity of the NaCas-HMP and NaCas-LMP complexes were conducive to the retention of β-carotene. In vitro digestion experiments showed that the complex emulsions could pH-responsively release β-carotene in the gastrointestinal tract at a release rate of more than 70%, and the bioaccessibility of β-carotene in complex emulsions was up to 72.6%, 76.6% and 80.2% respectively, all of which were related to the interfacial and rheological properties of the complex emulsion. These results illustrate the relationship between the physicochemical properties of the complex emulsions and the stability and digestibility of the emulsion system, which facilitate the design of an emulsion delivery system for bioactive substances.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Nan Ma,Rong Li,Gui-Fang Zhang,Ruo-Han Gao,Dong-Jie Zhang
Quinoa β-glucan (QBG) has shown potential benefits in treating type 2 diabetes mellitus (T2DM); however, comprehensive evaluations of its effects remain limited. This study investigates the impact of QBG—derived from hot water extraction (Q-) and microbial fermentation enrichment (Q+)—on serum glucose levels, lipid profiles, appetite-regulating hormones, fecal short-chain fatty acids (SCFAs), and gut microbiota composition and function in streptozotocin/high-fat diet (STZ/HFD)-induced T2DM mice. The results indicate that QBG treatment significantly reduced fasting blood glucose, insulin levels, triglycerides (TG) and total cholesterol (TC), while concurrently increasing high-density lipoprotein cholesterol (HDL C) levels. Additionally, liver and pancreatic function improved, as evidenced by decreased levels of malondialdehyde (MDA), aspartate transaminase (AST), and alanine transaminase (ALT). SCFA levels were significantly higher in QBG-treated groups compared to MC group. QBG treatment also reduced the abundance of Firmicutes and Patescibacteria, along with the Firmicutes/Bacteroidota ratio, while increasing levels of Bacteroidota and Actinobacteria. These findings suggest that QBG can regulate the dysbiosis of SCFAs production in T2DM mice and may indirectly modulate the secretion of appetite-regulating hormones by influencing gut microbiota composition. Furthermore, PICRUSt analysis revealed that QBG treatment, particularly Q + _H, could enhance disrupted metabolism and improve gut microbiota functions, helping restore normal physiological function.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Tao Gou,Wenxiu Li,Shenglin Chen,Chao Yi,Yu Guo,Zheng Cao,Lei Zhou,KangJu Lee,Mingrui Chen,Yaowen Liu
In this study, a hydrophobic and antibacterial pad was prepared to preserve Channel Catfish ( Ictalurus punctatus ). The pad composite the microfibrillated cellulose and β-cyclodextrin/nisin microcapsules. The hydrophobic pad ensures a dry surface in contact with the fish, reducing microbial contamination. The pad has a low density and high porosity, making it lightweight and suitable for packaging applications, while also providing a large surface area for antibacterial activity. Results demonstrated that this antibacterial pad exhibits an ultralow density of 9.0 mg/cm 3 and an ultrahigh porosity of 99.10%. It can extend the shelf life of Channel Catfish fillets to 9 days at 4 °C, with a total volatile base nitrogen below 20 mg/100 g. The study proposes a novel solution for preserving aquatic products by combining antibacterial substances with the natural base material aerogel. This approach also extends the utilization of aerogel and nisin in food packaging.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2024
·Vol. 104
·期 11
·6584-6593
·
IF 3.3
Junli Lv,Mengmeng Tan,Jiali Zou,Yueying Yun,Yan Ren,Fucheng Guo,Guoze Wang
BACKGROUND The biological functions of ferulic acid (FA) have garnered significant interest but its limited solubility and stability have led to low bioavailability. Hydroxypropyl- β -cyclodextrin (HP- β -CD), with its distinctive hollow structure, offers the potential for encapsulating hydrophobic molecules. The formation of an inclusion complex between FA and HP- β -CD may therefore be a viable approach to address the inherent limitations of FA. To investigate the underlying mechanism of the FA/HP- β -CD inclusion complex formation, a combination of spectral analyses and computer simulation was employed. RESULTS The disappearance of the characteristic peaks of FA in Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) confirmed the formation of an inclusion complex between FA and HP- β -CD. Thermogravimetry-derivative thermogravimetry (TG-DTG) studies demonstrated that the thermal stability of FA was enhanced due to the encapsulation of FA within HP- β -CD. Molecular dynamics simulation also provided evidence that FA successfully penetrated the HP- β -CD cavity, primarily driven by van der Waals interactions. The formation of the complex resulted in more compact HP- β -CD structures. The bioavailability of FA was also strengthened through the formation of inclusion complexes with HP- β -CD. CONCLUSIONS The findings of this study have contributed to a deeper understanding of the interactions between FA and HP- β -CD, potentially advancing a delivery system for FA and enhancing the bioavailability of insoluble active components. © 2024 Society of Chemical Industry.
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