ORGANIC & BIOMOLECULAR CHEMISTRY
·
2026
·
IF 2.8
Lin Chen,Shuang Xu,Zhiming Chen
Direct α-C(sp3)−H arylation of 4-aminopyrazolones involving arynes, enabled by dual phase-transfer catalysis and ultraviolet irradiation, has been developed. The dual phase-transfer catalytic pathway powered by triaminocyclopropenium salts gave rise to the generation of structurally diverse 4-amino-4-arylpyrazolones in moderate to excellent yields, while the photochemical protocol furnished the products with moderate yields. Notably, these products are tentatively proposed as candidates for carbamate pesticides based on the preliminary structural analysis. The synthetic potentials of the methods were shown by gram-scale reactions, and the possibilities for greater structural diversity of the product were highlighted by derivatizations. The results of control experiments indicated that the timely capture of the transient arynes by the activated 4-aminopyrazolone nucleophiles is the key to the success of the reactions.
✓ 已验证
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2026
·
IF 7.3
Ziyao Li,Nan Wang,Bo Li,Xuzhuo Sun,Jing Chen
The electrocatalytic conversion of 5-hydroxymethylfurfural (HMF) to 2,5-furandicarboxylic acid (FDCA) holds significant research value and broad application potential. The catalyst, comprising cobalt nanoparticles (Co NPs) encapsulated within nitrogen-doped carbon nanotubes (NCNTs) grown in situ on carbon paper (Co@NCNT/CP), was successfully developed. The catalyst achieved paired electrocatalytic reactions for HMF oxidation coupled with 4-nitrophenol (4-NP) reduction. Both HMF and 4-NP exhibited conversion rates exceeding 99%. The FDCA yield and selectivity reached 97%, with a Faradaic efficiency of 99%. The 4-aminophenol (4-AP) yield and Faradaic efficiency were 98%. Mechanistic studies revealed that electrochemically generated Co3+ species serve as the primary active sites for HMF oxidation. Electron interactions between the NCNTs and encapsulated Co NPs further stabilize the cobalt valence state. The structure facilitates HMF adsorption on Co NP surfaces, primarily via the Eley–Rideal mechanism, promoting catalytic conversion under high HMF concentrations. The paired electrochemical system exhibited outstanding catalytic performance and faster reaction rates for synergistic HMF oxidation and 4-NP hydrogenation than for individual reactions.
✓ 已验证
产品关联
DOI
高影响因子
PROCESS BIOCHEMISTRY
·
2026
·
IF 4.4
Liyang Xu,Yujia Wang,Feiyu Yan,Wenjing Pan,Yingjia Tong,Jinsong Gong,Xiaomei Zhang,Jinsong Shi,Zhenghong Xu,Hui Li
Mycolicibacterium is a crucial industrial host for steroid biotransformation, yet its genetic engineering has long been limited by inefficient homologous recombination (HR) and strong non-homologous end joining (NHEJ) activity. Here, we established and optimized a suicide plasmid-based genome editing system for Mycobacterium sp. LY-1. The constructed vector p2NILB, combining kanamycin selection with sacB-mediated counter-selection, enabled precise and markerless allelic exchange. Suppression of NHEJ through deletion of ku or ligD , together with optimization of homology arm length, increased gene knockout efficiency to over 70%. This optimized platform was subsequently applied to metabolic engineering of sterol degradation to enhance 9α-hydroxyandrostenedione (9α-OH-AD) biosynthesis. Sequential deletion of five kstD isoenzymes blocked downstream steroid degradation, with kstD1 identified as the major contributor to product loss. Functional analysis revealed KshA2 as the key 9α-hydroxylase, and its overexpression increased the 9α-OH-AD titer to 3.41 g/L. In addition, deletion of the branched-chain acid transporter gene mmpL3 further enhanced 9α-OH-AD production to 4.19 g/L. To overcome substrate utilization limitations under high phytosterol loading conditions, a soybean oil‑Tween 80 solvent system overcame substrate limitation, enabling 15.71 g/L 9α‑OH‑AD from 30 g/L phytosterols. This study provides a robust genome editing framework and an effective strategy for engineering steroid-producing actinobacteria.
✓ 已验证
DOI
高影响因子
BIOCHEMICAL PHARMACOLOGY
·
2026
·
IF 6.5
Ping Lv,Dandan Li,Wei Song,Jizhe Wang
Allergic rhinitis (AR) is a chronic inflammatory disorder characterized by type 2 T helper (Th2) cell-dominant immune responses. NEDD4 like E3 ubiquitin protein ligase (NEDD4L) plays a role in regulating T cell activation and Th cell differentiation. Dipeptidyl peptidase 4 (DPP4) is involved in Th2 inflammatory responses in AR. In this study, NEDD4L was found to be downregulated in peripheral blood CD4 + –enriched T cells from AR patients. In a BALB/c mouse model of AR, established by intraperitoneal sensitization with 25 μg ovalbumin (OVA) followed by intranasal challenge with 500 μg OVA, NEDD4L expression was also reduced in splenic T cells enriched in CD4 + populations. T cell-specific overexpression of NEDD4L, achieved via tail vein injection of lentivirus carrying the CD3δ promoter, significantly alleviated AR symptoms, as shown by reduced sneezing and nose-wiping frequency, and decreased serum levels of OVA-specific immunoglobulin E (IgE) and histamine. Moreover, NEDD4L overexpression attenuated nasal mucosal thickening, inflammatory cell infiltration, and goblet cell hyperplasia. In vitro , lentiviral-mediated NEDD4L overexpression in T cells, enriched in CD4 + populations, is associated with suppression of Th2-linked inflammation, as indicated by decreased GATA-binding protein 3 (GATA-3) and JunB proto-oncogene (JunB) protein levels, reduced secretion of Th2 cytokines, and a lower proportion of CD4 + IL-4 + cells. Mechanistically, NEDD4L ubiquitinates DPP4 at the K583 site to promote its degradation, and DPP4 overexpression reversed the anti-inflammatory effects of NEDD4L. Collectively, NEDD4L is consistent with acting as a negative regulator of Th2-linked inflammation, potentially via ubiquitination-mediated degradation of DPP4.
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2026
·
IF 7.8
Jingye Yao,Qingrong Zhang,Mengnan Zhang,Zhao Zhou,Shentian Zou,Shixuan Chen,Yuhui Cai,Yi Zhang
Chronic wound healing remains a persistent challenge clinically, particularly in diabetic wounds, where hyperglycemia drives sustained activation of the AGE-RAGE-associated signaling within the wound bed, precipitating excessive ROS generation and persistent inflammation that together stall tissue repair and erode quality of life. To address this, we developed a core-shell microneedle (GS + IL-4@MN) patch loading Gymnema sylvestre (GS) extract and interleukin-4 (IL-4) as a promising therapeutic strategy. The patch comprised a 10 × 10 array of conical needles and the optimized shell-loading concentration of GS was determined to be 1.0 mg/mL. The GS-loaded outer shell can suppress the release of pro-inflammatory mediators, scavenge ROS, and strengthen antioxidant defenses, thereby attenuating local inflammation; the IL-4-encapsulated core induces macrophage polarization toward the M2 phenotype, mitigates the pro-inflammatory microenvironment, and modulates T-cell immunity, while enhancing cellular proliferation and migration to support tissue regeneration. In diabetic wound models, GS + IL-4@MN achieved wound-closure rates of 64.72 ± 6.3% at day 3 and 85.80 ± 3.6% at day 7, and re-epithelialization reached 91.52 ± 2.1% by day 14. Moreover, collagen deposition increased to 79.38 ± 4.3%, CD31-positive neovascularization reached 70.50 ± 3.7%, and the M1/M2 ratio decreased to 0.3, indicating marked inflammatory resolution and regenerative activation. In summary, this core-shell MN system achieves multidimensional regulation of the pathological microenvironment of diabetic wounds through a dual mechanism: outer-layer inflammatory suppression and inner-layer microenvironment reset, and offers a new avenue for promoting diabetic wound healing.
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2026
·
IF 5.1
Yin Pang,Zhuolin Qi,Lei Cao,Wei Qi,Junfeng Mao,Yukun Huang,Haifeng Cong,Xingang Li,Jiawei Wen
Dual-site modulated P204-P507/XAD-4 resin separates Pt/Co efficiently. • Alkaline activation of P-OH groups significantly boosts Co(II) capture. • HFIP effectively shields P=O sites to suppress Pt(IV) co-adsorption. • The Co/Pt separation factor achieves a nearly ten-fold improvement. • DFT and XPS reveal decoupled pathways via synergy-through-inhibition. The efficient recovery of platinum (Pt) and cobalt (Co) from spent proton exchange membrane fuel cell (PEMFC) catalysts is vital for the sustainable hydrogen economy, yet their deep separation from acidic leachates is hindered by the inherent affinity property of organophosphorus extractants. In this study, a dual-site regulation strategy was developed using a synergistic P204-P507/XAD-4 solvent-impregnated resin (SIR). By combining alkaline activation to enhance P–OH cation-exchange capacity (boosting Co(II) uptake from 40.19 ± 0.31 to 47.26 ± 0.58 mg/g at pH 7.0) as well as the hexafluoroisopropanol (HFIP) masking to selectively shield P=O sites via competitive hydrogen bonding (suppressing Pt(IV) adsorption from 7.60 ± 0.66 to 3.78 ± 0.47 mg/g at pH 3.0), the competing adsorption pathways were effectively decoupled. Experimental results showed that the dual-modified resin achieved a Co(II) adsorption capacity of 47.26 ± 0.58 mg/g and an exceptional separation factor (SF Co/Pt ) of 248.85 ± 10.19 at pH 7.0, representing an approximately ten-fold improvement over the unmodified composite. Mechanistic investigations via XPS and DFT calculations indicated that Co(II) is predominantly captured through P–OH sites, while the binding energy shifts in P=O groups are primarily attributed to a secondary inductive effect rather than direct coordination. Furthermore, in continuous fixed-bed operation, the resin exhibited a wide separation window with Co(II) breakthrough delayed to 55 bed volumes, delivering a Pt-enriched effluent with 99.1% purity at 97.0% recovery, while the retained Co(II) was quantitatively eluted at 96.6% purity. The resin also retained over 90% of its initial Co(II) adsorption efficiency after three consecutive adsorption–desorption cycles. and offering a mechanism-driven approach for precision metal adsorbent design.
✓ 已验证
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2026
·Vol. 14
·期 2
·122147
·
IF 7.5
Kai Wu,Shubing Yang,Jianren Ouyang,Yue Sun,Zhaojie Wang,Peizeng Yang,Yuefei Ji,Jing Chen,Junhe Lu
This study demonstrates the efficacy of the peracetic acid (PAA)/bromide (Br - ) system for degrading 4-chlorophenol (4-CP) under freezing conditions, highlighting both performance and associated byproduct risks. Freezing significantly enhanced 4-CP removal across a range of Br - concentrations, driven by freeze-concentration effects. The reaction followed pseudo-first-order kinetics, with the rate constant ( k ) increasing with Br - concentrations. This enhancement is attributed to solute enrichment within the unfrozen brine phase, which promotes bromine activation and accelerates oxidation kinetics. Mechanistic analyses indicate that free bromine specie (HOBr) is responsible for 4-CP transformation, leading to the formation of brominated intermediates. In addition to enhanced contaminant removal, freezing also promoted the formation of brominated disinfection by-products (DBPs), including haloacetic acids (HAAs) and trihalomethanes (THMs). Notably, the dichloroacetic acid (DCAA) yields increased more than twentyfold compared to room temperature. These results demonstrate a dual effect of freezing in bromide-containing systems, simultaneously enhancing pollutant degradation and DBP formation. The findings highlight the importance of considering freezing-induced chemical processes in cold-region water treatment and environmental risk assessment.
✓ 已验证
产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2026
·
IF 9.1
Jiaxi Jin,Yuyang Xiao,Hao Lai,Ruixing Fu,Peilun Shen,Dianwen Liu,Xinyi Liang,Bo Peng
The efficient separation of chalcopyrite from sphalerite remains a significant challenge in flotation due to their similar surface properties. In this study, Trisodium 4-carboxy-5-mercapto-3-hydroxy-isothiazole (TCMHI) was introduced as a novel, eco-friendly, and selective organic depressant for sphalerite. Micro-flotation tests demonstrated that TCMHI (73 mg/L) could selectively depress sphalerite flotation (recovery <14%) at pH 7.0 while maintaining high chalcopyrite recovery (> 94%). Successful separation was achieved in artificially mixed mineral tests, yielding a concentrate with a Cu grade of 32.01% and a recovery of 96.65%. To validate its industrial applicability, bench-scale flotation tests were conducted on a real Cu Zn sulfide ore. The results showed that TCMHI achieved metallurgical performance comparable to the conventional depressant zinc sulfate (ZnSO 4 ), maintaining a copper recovery of 71.32% while effectively suppressing zinc recovery to 21.18% in the concentrate. Surface characterization techniques, including contact angle, zeta potential, and Atomic Force Microscopy (AFM), revealed that TCMHI preferentially adsorbs onto the sphalerite surface, forming a dense hydrophilic layer that hinders collector adsorption. X-ray Photoelectron Spectroscopy (XPS) and Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) analyses confirmed that TCMHI chemically adsorbs on sphalerite through the formation of stable Zn-TCMHI surface complexes. Density Functional Theory (DFT) calculations further elucidated the mechanism, showing that TCMHI forms a stable five-membered chelate ring with surface Zn atoms via its nitrogen and endocyclic sulfur atoms. Crucially, the adsorption energy of TCMHI on sphalerite (−209.76 kJ/mol) was found to be significantly more negative than that of the collector (−95.88 kJ/mol), explaining its superior depression performance. This work provides a promising reagent for the sustainable and efficient flotation separation of Cu Zn sulfide ores.
✓ 已验证
产品关联
DOI
高影响因子
POLYMER
·
2026
·
IF 5.1
Yao Long,Jiahao Zhou,Xupeng Han,Min Zhang,Yayan Wang,Jiaping Tan,Meilin Liu,Lijun Li,Kun Liu
To develop myrcene-based polymers with both functionalization characteristics and controllable structure, this paper employs 1-(vinylbenzyl) piperidine (VBMP) as a tertiary amine functional monomer and achieves the controlled copolymerization of VBMP with β -myrcene (MY) under mild conditions above room temperature in cyclohexane using living anionic polymerization. By leveraging the dual role of VBMP as both a comonomer and a polarity regulator, and optimizing the monomer feed ratio, poly(MY- co -VBMP) copolymers with controllable molecular weight and narrow molecular weight distribution (1.11 < PDI < 1.23) are successfully prepared. 1 H NMR and FTIR characterizations confirm that VBMP is uniformly inserted into the polymer backbone via in-situ copolymerization, achieving a quantitative functionalization. The reactivity ratios are determined using the Fineman-Ross and Kelen-Tüdős methods, yielding r MY = 4.62-4.80 and r VBMP = 0.43-0.54. Moreover, DSC analysis shows that with increasing VBMP content, the glass transition temperature ( T g ) of the copolymers gradually rises from −18 °C to 25 °C, accompanied by significantly enhanced chain segment regularity. In addition, the LAP chain-end active center coordination mechanism and the N-Li coordination synergy mechanism within the polymer chain are proposed to rationalize the experimental results. Overall, this polymerization system not only exploits the weak regulatory effect of the tertiary amine group of VBMP on anionic polymerization but also achieves quantitative functionalization of β -myrcene copolymers, providing an efficient route to functional materials possessing both elastic properties and reactive sites.
✓ 已验证
DOI
高影响因子
BIOMASS & BIOENERGY
·
2026
·
IF 7
Jing Bai,Mengqi Wang,Yang Xiao,Xiangyu Cheng,Weiyu Chen,Haoran Wu,Chun Chang
A single-step removal of γ-carbinol from lignin β-O-4 linkages was reported. • Key parameters for γ-carbinol removal were systematically screened and optimized. • Ru/C system enabled efficient upgrading of monophenols from birch-derived bio-oil. • The selectivity and yield of 2,6-dimethoxy-4-ethylphenol reach 53.02% and 39.33%. • A promising route for lignin valorization was proposed. Development of efficient strategies for converting the C3 β-O-4 motif of lignin into a simplified C2 β-O-4 structure is of great significance, yet it remains a formidable challenge. Herein, we report the use of Ru/C as a catalyst to convert α-hydroxy-free C3 β-O-4 fragments into C2 β-O-4 fragments via a one-step strategy for the removal of the γ-carbinol group. A series of lignin-derived phenolic model compounds were selected to systematically investigate the effects of key reaction parameters, including temperature, reaction time, catalyst dosage, and substituent structure on the removal of the γ-carbinol group. On the basis of the obtained reaction rules and optimized parameters, the Ru/C-catalyzed system was successfully applied to the upgrading of monophenolic components in bio-oil obtained from the depolymerization of authentic birch lignin. Under optimized reaction conditions, 4-(3-hydroxypropyl)-2,6-dimethoxyphenol in the bio-oil achieved a conversion of 74.18%, affording the target product 2,6-dimethoxy-4-ethylphenol with a selectivity of 53.02% and a yield of 39.33%. This study provides a novel and efficient approach for the valorization of lignin-derived bio-oil, laying a solid foundation for the sustainable utilization of lignin resources. Download: Download high-res image (376KB) Download: Download full-size image
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Lifang Zou,Zhaoming Wang,Xingguang Chen,Shasha Zheng,Feiran Xu,Kezhou Cai,Hui Zhou,Guiran Xiao,Baocai Xu
Saltiness–enhancing peptides are a novel class of salt substituents capable of partially replacing sodium chloride (NaCl) in food products. The current study describes the isolation and identification of saltiness–enhancing peptides from chicken skin collagen hydrolysate. Six of the peptides, including GERGPKGPKGETGPT (GT15), GPAGPPGARGQAG (GG13), GPPGPTGPA (GA9), GPPGPTGPAGER (GR12), VGPTGPAGPR (VR10), and VGSP (VP4), were found to enhance the saltiness intensity of a 0.35 % ( w / v ) solution of NaCl. These six peptides exhibited an umami taste, with GT15 and GR12 additionally having a salty taste. Molecular dynamics simulation revealed that these peptides can stably bind to the salty taste receptor transmembrane channel–like 4 via hydrogen bonds. In vitro studies revealed that these peptides exhibited antioxidant activities. These six saltiness–enhancing peptides can therefore be potentially applied for augmenting the saltiness of NaCl solution and partially replacing salt in food products while preventing the oxidative damage typically caused by high amounts of salt.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LUMINESCENCE
·
2025
·Vol. 40
·期 6
·e70219
·
IF 3
Junxia Wang,Ying Qin,Na Li,Yu He,Gongwu Song
2, 4-dichlorophenoxyacetic acid is a widely used pesticide, necessitating the development of a low-cost, reliable, and rapid detection method. In this work, BCNO quantum dots were synthesized by a one-step microwave method. A fluorescence “turn-off-on” probe for 2, 4-dichlorophenoxyacetic acid detection was developed using MnO 2 nanosheets due to their strong oxidation and broad absorption spectrum. MnO 2 nanosheets can quench the fluorescence of BCNO quantum dots through internal filter effect. Alkaline phosphatase hydrolyzes trisodium L-ascorbate-2-phosphate to produce ascorbic acid, which decomposes MnO 2 nanosheets, restoring the fluorescence of BCNO quantum dots. With the increase of alkaline phosphatase concentration, the ascorbic acid produced gradually increased; thus, the fluorescence intensity of BCNO quantum dots increased. 2, 4-dichlorophenoxyacetic acid inhibits alkaline phosphatase activity, preventing the decomposition of MnO 2 nanosheets and hindering fluorescence recovery. The probe exhibits a linear detection range of 1.0–40.0 mg/L and a detection limit of 0.067 mg/L. Color changes corresponding to 2, 4-dichlorophenoxyacetic acid concentration were analyzed using a smartphone application, establishing a digital image colorimetry method. The results demonstrated a good linear relationship within the range of 0–50 mg/L. The method was validated with real samples, demonstrating its utility in environmental and food safety monitoring.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）