JOURNAL OF NANOBIOTECHNOLOGY
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2024
·Vol. 22
·期 1
·1-18
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IF 10.6
Jin Jiale,Xia Xiaowei,Ruan Chengxin,Luo Zhiyuan,Yang Yiqi,Wang Dongyu,Qin Yifang,Li Dongdong,Zhang Yong,Hu Yihe,Lei Pengfei
Macrophage metabolism dysregulation, which is exacerbated by persistent stimulation in infectious and inflammatory diseases, such as diabetic infectious bone defects (DIBD), eventually leads to the failure of bone repair. Here, we have developed an injectable, macrophage-modulated GAPDH-Silence drug delivery system. This microsphere comprises chondroitin sulfate methacrylate (CM) and methacrylated gelatin (GM), while the dimethyl fumarate (DMF)-loaded liposome (D-lip) is encapsulated within the microsphere (CM@GM), named D-lip/CM@GM. Triggered by the over-expressed collagenase in DIBD, the microspheres degrade and release the encapsulated D-lip. D-lip could modulate metabolism by inhibiting GAPDH, which suppresses the over-activation of glycolysis, thus preventing the inflammatory response of macrophages in vitro. While beneficial for macrophages, D-lip/CM@GM is harmful to bacteria. GAPDH, while crucial for glycolysis of staphylococcal species ( S. aureus ), can be effectively countered by D-lip/CM@GM. We are utilizing existing drugs in innovative ways to target central metabolism for effective eradication of bacteria. In the DIBD model, our results confirmed that the D-lip/CM@GM enhanced bacteria clearance and reprogrammed dysregulated metabolism, thereby significantly improving bone regeneration. In conclusion, this GAPDH-Silence microsphere system may provide a viable strategy to promote diabetic infection bone regeneration.
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Communications Biology
·
2024
·Vol. 7
·期 1
·1-13
·
IF 5.2
Lai Cuixin,Yang Lina,Pathiranage Vishaka,Wang Ruizhao,Subach Fedor V.,Walker Alice R.,Piatkevich Kiryl D.
Genetically encoded fluorescent biosensors became indispensable tools for biological research, enabling real-time observation of physiological processes in live cells. Recent protein engineering efforts have resulted in the generation of a large variety of fluorescent biosensors for a wide range of biologically relevant processes, from small ions to enzymatic activity and signaling pathways. However, biosensors for imaging sulfate ions, the fourth most abundant physiological anion, in mammalian cells are still lacking. Here, we report the development and characterization of a green fluorescent biosensor for sulfate named Thyone. Thyone, derived through structure-guided design from bright green fluorescent protein mNeonGreen, exhibited a large negative fluorescence response upon subsecond association with sulfate anion with an affinity of 11 mM in mammalian cells. By integrating mutagenesis analyses with molecular dynamics simulations, we elucidated the molecular mechanism of sulfate binding and revealed key amino acid residues responsible for sulfate sensitivity. High anion selectivity and sensitivity of Thyone allowed for imaging of sulfate anion transients mediated by sulfate transporter heterologously expressed in cultured mammalian cells. We believe that Thyone will find a broad application for assaying the sulfate transport in mammalian cells via anion transporters and exchangers.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-15
·
IF 15.7
Liu Lu,Li Xiurong,Chen Yongxian,Gao Junbin,Jiang Yuejun,Ye Yicheng,Wang Peng,Peng Fei,Tu Yingfeng
Uricase (UOx)-based gout treatments are generally limited due in part to the accumulation of H 2 O 2 in an arthrosis environment characterized by a sluggish metabolism. Here we develop a self-cascade nanomotor with high efficiency toward simultaneous uric acid (UA) degradation and H 2 O 2 elimination on the basis of UOx and sodium citrate loading in amine functionalized hollow mesoporous silica nanoparticles (AHMSNs). Due to the inherent asymmetry of AHMSNs, the developed nanomotors can be actuated by ionic diffusiophoresis induced by the enzymatic UA degradation, thus enlarging the diffusion range within the joint cavity. H 2 O 2 generated during UA decomposition can be simultaneously eliminated by the loaded sodium citrate, resulting in a considerable improvement in mammalian cell viability. In vivo studies display powerful therapeutic effect in gouty models using this self-cascade nanomotor system. These results present the potential of nanomotors as active therapeutic agents for gout, providing a perspective on the application of enzyme-driven micro/nanomotors.
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NATURE
·
2024
·Vol. 631
·期 8019
·73-79
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IF 50.5
Kong Lingmei,Sun Yuqi,Zhao Bin,Ji Kangyu,Feng Jie,Dong Jianchao,Wang Yuanzhi,Liu Zirui,Maqbool Shabnum,Li Yunguo,Yang Yingguo,Dai Linjie,Lee Wanhee,Cho Changsoon,Stranks Samuel D.,Friend Richard H.,Wang Ning,Greenham Neil C.,Yang Xuyong
Light-emitting diodes (LEDs) based on metal halide perovskites (PeLEDs) with high colour quality and facile solution processing are promising candidates for full-colour and high-definition displays 1 , 2 , 3 , 4 . Despite the great success achieved in green PeLEDs with lead bromide perovskites 5 , it is still challenging to realize pure-red (620–650 nm) LEDs using iodine-based counterparts, as they are constrained by the low intrinsic bandgap 6 . Here we report efficient and colour-stable PeLEDs across the entire pure-red region, with a peak external quantum efficiency reaching 28.7% at 638 nm, enabled by incorporating a double-end anchored ligand molecule into pure-iodine perovskites. We demonstrate that a key function of the organic intercalating cation is to stabilize the lead iodine octahedron through coordination with exposed lead ions and enhanced hydrogen bonding with iodine. The molecule synergistically facilitates spectral modulation, promotes charge transfer between perovskite quantum wells and reduces iodine migration under electrical bias. We realize continuously tunable emission wavelengths for iodine-based perovskite films with suppressed energy loss due to the decrease in bond energy of lead iodine in ionic perovskites as the bandgap increases. Importantly, the resultant devices show outstanding spectral stability and a half-lifetime of more than 7,600 min at an initial luminance of 100 cd m −2 .
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Nature Communications
·
2024
·Vol. 15
·期 1
·1-19
·
IF 14.7
He Liangrui,Wang Liyang,Yu Xujiang,Tang Yizhang,Jiang Zhao,Yang Guoliang,Liu Zhuang,Li Wanwan
The poor 5-year survival rate for bladder cancers is associated with the lack of efficient diagnostic and treatment techniques. Despite cystoscopy-assisted photomedicine and external radiation being promising modalities to supplement or replace surgery, they remain invasive or fail to provide real-time navigation. Here, we report non-invasive fractionated photodynamic therapy of bladder cancer with full-course real-time near-infrared-II imaging based on engineered X-ray-activated nanotransducers that contain lanthanide-doped nanoscintillators with concurrent emissions in visible and the second near-infrared regions and conjugated photosensitizers. Following intravesical instillation in mice with carcinogen-induced autochthonous bladder tumours, tumour-homing peptide-labelled nanotransducers realize enhanced tumour regression, robust recurrence inhibition, improved survival rates, and restored immune homeostasis under X-ray irradiation with accompanied near-infrared-II imaging. On-demand fractionated photodynamic therapy with customized doses is further achieved based on quantifiable near-infrared-II imaging signal-to-background ratios. Our study presents a promising non-invasive strategy to confront the current bladder cancer dilemma from diagnosis to treatment and prognosis.
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Nano Research
·
2024
·
IF 9.5
Su Jihao,Yang Ling,Yu Dengbin,Dong Shaojun
A significant challenge in developing high-performance hybrid supercapacitors (HSCs) is the need to reasonably construct advanced architectures that consist of various components and exhibit superior electrochemical capacitance performance. The FeCoNi-layered double hydroxide (FeCoNi-LDH) porous material has a specific capacitance of 1960 F·g −1 when used as the anode material at 1 A·g −1 . The FeCoNi-LDH material exhibits nanoplates with a distinct spindle morphology on their surface. Due to the combined action of the three metals and abundant oxygen vacancies, they exhibit unique rate performance and cycle stability. The electronic structure of LDH and the regulation of oxygen vacancy were confirmed by density functional theory (DFT) calculations. This suggests that the strength of hydroxide can reduce the energy required for oxygen vacancy formation in FeCoNi-LDH nanosheets and enhance ion and charge transfer, as well as electrolyte adsorption on the electrode surface. The FeCoNi-LDH//activated carbon (AC) HSC has an energy density of 53.2 Wh·kg −1 at a power density of 800 W·kg −1 , surpassing other devices composed of comparable materials during the same timeframe. This study made significant advances in the design and synthesis of a ternary LDH porous structure with distinct oxygen vacancies, as well as its potential application in electrochemical energy storage.
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JOURNAL OF ELECTROCERAMICS
·
2024
·
IF 1.7
Chen Binbin,Hu Yang,Zhang Huazhang,Chen Wen,Zhou Jing
The fatigue behavior of Bi 0.5 Na 0.4 K 0.1 TiO 3 -based ceramics depends on the polarity. While the non-ergodic relaxor ceramics have large residual polarization but poor fatigue behavior, the ergodic relaxor ceramics have excellent fatigue resistance but tiny residual polarization. Therefore, obtaining ferroelectric ceramics with high residual polarization and excellent fatigue resistance is challenging due to the trade-off between non-ergodic relaxor and ergodic relaxor. Here, we modulate the free energy barrier by doping relaxant Nb to achieve the coexistence of non-ergodic and ergodic relaxor phases. At 0.6% Nb doping, the residual polarization is large at 2 P r = 49.3 µC/cm 2 , increased to 54.23 µC/cm 2 after 10 2 cycles and decreased to 53.04 µC/cm 2 after 10 5 cycles, indicating good fatigue resistance behavior. The large residual polarization is due to the metastable ferroelectric state, while the excellent fatigue resistance may be attributed to the field-induced ferroelectric-relaxor phase transition.
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MOLECULAR NEUROBIOLOGY
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2025
·
IF 4.3
Yan Qian,Li Xiao-Jun,Wang Qi-Qi,Jia Wei,Wang Shu-Ling
Approaches of promoting a neural milieu permissive for plasticity and resilience against neuronal injury are important strategies for the treatment of a range of neurological disorders. Fibroblast growth factor 21 (FGF21) which is known for its role as a potent regulator of glucose and energy metabolism has also proved to be neuroprotective against various mental diseases. However, the underlying molecular mechanisms remain elusive. Here, we report a study of the neuroprotective effects of FGF21 by promoting 5-HT 1A R-FGFR1 heteroreceptor formation and triggering MEK 1/2 -ERK 1/2 signaling pathway in normal or abnormal neurological conditions. First, the in vitro cellular experiments demonstrated that FGF21 exerted a protective effect against glutamate-induced cytotoxicity and promoted cell differentiation and growth. Then, in wild-type and FGF21 −/− mice, exogenous FGF21 promoted FGFR1-5-HT 1A R heteromers formation in the CA3 and dentate gyrus region of the hippocampus and activated MEK 1/2 -ERK 1/2 signaling. Coordinately, FGF21 exerted similar influences in the hippocampi of IBA-induced neurological injury mice or combined stress-exposed mice. Besides, FGF21 treatment activated the phosphorylation of FGFR1 and elevated the expression of synaptophysin in these mice with neurological injury or combined stress exposure. These results illustrated that FGF21 alleviated neurological impairment through FGFR1-5-HT 1A R heteromer and ERK 1/2 signal activation and suggested that the regulation of FGFR1-5-HT 1A R heteromers and MEK 1/2 /ERK 1/2 pathway may play a key role in mediating the neuroprotective effects of FGF21 against various neurodegeneration conditions.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-7
·
IF 15.7
Yao Aiping,Xu Hongliang,Shao Kuizhan,Sun Chunyi,Qin Chao,Wang Xinlong,Su Zhongmin
Soft porous crystals, recognized as the third generation of smart porous materials, can undergo structural deformations in response to external stimuli, such as temperature, pressure, and guest molecules. Currently, the dynamic phase transformations of soft porous crystals are predominantly determined through quantitative modeling based on gas adsorption and powder X-ray diffraction. Herein, we investigate the single-crystal-to-single-crystal structural transformation of covalent organic soft porous crystal modeled on COF-300 and identified nine distinct conformational isomers induced by different guest molecules at room and high temperatures. Notably, COF-300 can maintain its single-crystal structure even at 280 °C and efficiently absorbs polycyclic aromatic hydrocarbons in their molten state. The kinetics of structural transformations among conformational isomers are investigated by combining PXRD and theoretical calculations. The structural transformation from a high-energy state to a low-energy state is a rapid, energetically favorable process, while the reverse transformation is a slow process driven by concentration gradients.
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JOURNAL OF NANOPARTICLE RESEARCH
·
2024
·Vol. 26
·期 8
·1-18
·
IF 2.1
Liu Xiangyang,Wang Jin,Fang Longhui,Ban Yutao,Li An,He Maogang
Membrane is a potential candidate to solve the challenge of air separation to meet the huge industrial demand of N 2 or O 2 . In this contribution, four types of prominent metal organic frameworks (MOFs) with different pore sizes and series, including ZIF-8 (3.4 Å), ZIF-71 (4.2 Å), UiO-66 (6 Å) and MIL-53(Al) (16.3 Å) were synthesized, and used as a nanoporous filler combined with polydimethylsiloxane (PDMS) substrate to prepare MOFs/PDMS mixed matrix membranes (MMMs) for air separation, which were compared to derive the optimal MOFs with high O 2 /N 2 selectivity ( α O2/N2 ) and O 2 permeability ( P O2 ). Gas permeation test was done to analyze the influences of MOFs content, feed gas pressure and gas composition on the O 2 /N 2 separation performance. 10 wt% ZIF-71/PDMS MMMs exhibits the optimal separation performance ( α O2/N2 = 3.14, P O2 = 630 Barrer), while pressure has small effect on O 2 /N 2 separation performance. P O2 is higher under pure gas test conditions, while α O2/N2 is better under mixed gas conditions, which is caused by the competitive adsorption-diffusion of O 2 and N 2 molecules. Further, ZIF-71/PDMS/PVDF composite membrane that could produce 30 vol% oxygen-enriched permeate stream was prepared, and the P O2 (1047 Barrer) is improved by 66% compared with MMMs.
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npj Flexible Electronics
·
2024
·Vol. 8
·期 1
·1-16
·
IF 12.3
Deng Mengkang,Du Chentao,Fang Jiayuan,Xu Changshun,Guo Chenhui,Huang Jiantao,Li Kang,Chen Lei,Zhang Yuan-Ting,Chang Yu,Pan Tingrui
Continuous hemodynamic monitoring in a wearable means can play a crucial role in managing hypertension and preventing catastrophic cardiovascular events. In this study, we have described the fully wearable tonometric device, referred to as flexible adaptive sensing tonometry (FAST), which is capable of continuous and accurate monitoring of hemodynamic parameters within the medical-grade precision. In particular, the FAST system integrates a 1 × 8 unit array of highly sensitive and highly flexible iontronic sensing (FITS) with 1 mm spatial resolution and a closed-loop motion system. The flexible tonometric architecture has been used to determine the radial arterial position with high sensitivity and high conformability, which simplifies the biaxial searching process of the traditional applanation tonometry into a highly efficient uniaxial applanation while keeping the medical-precision assessments. Importantly, a self-calibration algorithm can be automatically implemented during the applanation process, from which the intra-arterial blood pressure wave can be continuously predicted within the medical-grade precision, and subsequently, multi-parametric hemodynamic analysis can be performed in real-time. Experimental validations on health volunteers have demonstrated that the FAST measurements are all within the required accuracy of the clinical standards for continuous pulse wave assessments, blood pressure monitoring as well as other key hemodynamic parameter evaluations. Therefore, the FAST system, by integrating the flexible iontronic sensing array, provides a real-time, medical-grade hemodynamic monitoring solution in a continuously wearable manner, from which remote patient-centered monitoring can be delivered with both medical precision and convenience.
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JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2024
·
IF 2.6
Hui Yuchen,Fei Cheng,Zhang Zhongguo,Fan Shuang,Wang Huixin,Fan Wei,Chang Wei
This study fabricated a flexible and washable self-powered biosensor—based on cellulose textiles—for sensing glucose in sweat. The sensor is integrated with T-shirts for practical use. The cellulose fiber was modified with carbon nanotubes and reduced oxide graphene to decrease its electron transfer resistance and ohmic resistance. Due to their low resistance, the bioelectrodes display high electrocatalytic efficiency of glucose oxidation and oxygen reduction. The biosensors are assembled by packaging the bioanodes and gel electrolytes together. The assembled biosensors have a low limit of detection (6.7 µM) and a wide linear range (0.02–0.5 mM). The great sensing performances are strongly associated with the location of bioanodes and biocathodes. The outer biocathode protects the central bioanode that comes from oxygen and ensures the structural integrity of the device during bending cycles. It also reduces the damage caused by the washing. The biosensor also exhibited selectivity, repeatability, and great stability in different pHs and during long-term storage. Finally, the average recoveries in real sweat are 104.9 to 106.0%. Thus, the flexible and washable self-powered biosensors have great potential for diabetes diagnosis at an early stage by sensing glucose in sweat. Graphical abstract
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危险品化学品经营许可证（带存储）