Advanced Materials Interfaces
·
2022
·Vol. 9
·期 16
·2102590
·
IF 6.389
Bin Zhou,Guohua Li,Mingxi Wu,Zhangkai Zhou,Xin Cai,Jingxuan Cai,Jianhua Zhou
A platform for real-time monitoring and comprehensively studying the adhesion process of microorganisms onto material surfaces as well as their bioactivity on the surface is very helpful to reveal the microbial contamination mechanism on drainage catheters, intravascular stents, and implanted sensors. Herein, a multifunctional sensor based on localized surface plasmon resonance of gold nano-match head arrays (GNMAs) is developed for monitoring the adhesion behavior of microorganisms (such as Candida albicans , i.e., C. albicans ) as well as its inhibitory activity. The sensor is fabricated through soft lithography processes using nanopatterned polydimethylsiloxane stamps, and modified with polymers of different charges for investigating the adhesion of C. albicans onto different charged surfaces. The spectroscopic study shows that C. albicans are more favorable to positively charged surfaces than negatively charged ones, which is also proven by the fluorescence images under microscope. Moreover, the authors demonstrate that the GNMA modified with poly- l -lysine (PLL) can effectively kill the adhered C. albicans due to the membrane destruction and the reactive oxygen species induced by PLL. These unique advantages suggest that the sensor is capable as both an antifungal study system and a real-time monitoring platform for antifungal performance evaluation.
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Food Chemistry-X
·
2025
·
IF 8.2
Haibin Yuan,Yanlin Li,Jianhang Tu,Tianyang Wang,Kaixian Zhu,Hongfeng Jia,Huachang Wu,Ping Dong
Since the key aroma compounds and bioactive profiles of Phyllanthus emblica fruit wine (PFW) driven by different yeast strains remain unclear, this study aimed to systematically evaluate the effects of five Saccharomyces cerevisiae strains on physicochemical properties, bioactive components, and volatile organic compound (VOC) profiles in PFW. Using an integrated flavoromics approach with chemometric analysis, we identified 110 VOCs. FR and D254 enhanced ester concentrations and antioxidant activity respectively, whereas strain L2323 significantly altered aroma complexity by promoting specific esters and alcohols. Multivariate analyses identified methyl nitrate and n-propyl acetate as discriminant markers among strains. Aroma-activity analyses further highlighted ethyl hexanoate, isoamyl acetate, and n-propyl acetate as major aroma contributors with distinctive strain-dependent distributions. These findings provide critical guidance for yeast strain selection and fermentation optimization to improve PFW quality, offering new insights for the development of functional fruit wines.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2025
·
IF 3.5
Yining Hao,Shishuai Yu,Bo Peng,Liping Qu
BACKGROUND Phyllanthus emblica L. is a medicinal and edible plant renowned for its potent antioxidant and anti-inflammatory activity. However, the digestive stability, bioaccessibility, and intestinal protective effects of its polyphenolic components remain unclear. This study aimed to evaluate systematically the in vitro digestion behavior, antioxidant capacity, and anti-colitis potential of P. emblica extract (PE). RESULTS The bioaccessibility of total phenolics in PE was 92.03%, indicating high digestive stability, whereas the bioaccessibility of flavonoids was 42.69%, indicating partial degradation during digestion. Liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS) identified 3- O -galloylmucic acid and gallic acid as the dominant and stable phenolics throughout all digestive phases. Phyllanthus emblica L. retained strong antioxidant capacity across the oral, gastric, and intestinal stages. In a zebrafish model of trinitrobenzenesulfonic acid (TNBS)-induced inflammatory bowel disease, PE significantly ameliorated intestinal injury, reduced gut lumen expansion, inhibited neutrophil recruitment, and restored goblet cell numbers. Gene expression analysis showed a dose-dependent downregulation of pro-inflammatory cytokines ( tnf-α, il-1β, il-6) ( P < 0.05). CONCLUSION Phyllanthus emblica L. extract exhibited high digestive stability and polyphenol bioaccessibility while maintaining antioxidant activity. Its pronounced anti-inflammatory and mucosal-protective effects in vivo provided evidence supporting the potential application of P. emblica as a functional food ingredient for intestinal health and inflammatory bowel disease prevention. © 2025 Society of Chemical Industry.
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FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Jinqian Pang,Wenli Zhao,Yu Wang,Yan Li,Hong Wang,Weimin Zhang,Wenjuan Liang
Nephelium lappaceum L. is a tropical fruit with a characteristic hairy appearance, whose peel is used to reduce fever and diarrhea, while its leaves are employed to mitigate headaches. These advantages are mostly attributed to the accumulation of phenolic-related compounds in N. lappaceum . To investigate phenols accumulation and transformation in N. lappaceum peel, leaves and branches, this study investigated the major metabolites and their antioxidant and xanthine oxidase (XO) inhibitory activities. The results showed that the characteristic metabolites in peel, leaves and branches reflected a conversion process from simple phenols (C 6 ) to phenylpropanoids (C 9 ), and then to flavonoids (C 12 ). The peel extract exhibited the highest antioxidant activity, driven by its predominant simple phenols, gallic acid ( Ph7 ). The branch extracts displayed the strongest XO inhibitory activity, primarily attributed to the flavonoids: kaempferol 7- O - α - L -rhamnopyranoside ( Fl9 ), miquelianin ( Fl34 ) and procyanidin B2 ( Fl8 ). This study provides new directions for precisely utilization of N. lappaceum by-products.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Kai Yang,Yueshan Huang,Liyi Yao,Bin Huang,Weimin Zeng,Jin Huang
D-amino acid oxidase (DAAO) has long been recognized as a promising catalyst for chiral amino acid biosynthesis owing to its pronounced stereospecificity. However, its limited thermostability restricts industrial utility and poses challenges for protein engineering. Herein, we engineered a DAAO variant, M5 (S42D/T84D/H138N/A193R/E195Y/C234L), via a ProteinMPNN-guided multiple codon saturation mutagenesis approach. This variant exhibited superior catalytic efficiency (27.3 mM −1 s −1 , 29-fold relative to the starting enzyme) toward the non-natural compound D-glufosinate (D-PPT) and enhanced thermostability (519 min half-life at 50 °C, 208-fold relative to the starting enzyme). The evolution also expanded the enzyme's substrate scope toward several D-amino acids with different properties. The variant was proven able to completely transform 500 mM D-PPT in 7 h at a 100 mL scale with excellent stereospecificity. Molecular dynamics (MD) simulations gave the insight that the enhanced performance stems from altered surface charges and intramolecular/intermolecular networks. This strategy thus holds potential for engineering other enzymes involved in the biosynthesis of valuable products while reducing the screening costs during directed evolution.
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ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Jun Liu,Yan Tang,Xiangyun Lin,Cheng Zhang,Nan Ma,Lei Wang,Wei Qu,Meiyu Shao,Yuxia Zhao,Wei Zhang
Thrombus formation associated with blood-contacting catheters remains a prevalent clinical issue. Antithrombotic coatings capable of inhibiting coagulation activation without interfering with hemostasis are highly desired. Herein, we constructed thick and densely packed poly(ε-acryloyl l-lysine) (p(LysAA)) zwitterionic brushes on the inner wall of chemical inert polyethylene (PE) catheters via a recently developed universal, substrate-independent coating strategy that enables facile scaling-up for catheters with complex geometries. The densely packed l-lysine-derived zwitterionic brushes exhibited ultrahydrophilic properties, with water contact angles below 10°. As expected, fouling by bovine serum albumin, fibrinogen, bacteria, platelets, and red blood cells was effectively suppressed. Moreover, the brushes demonstrated excellent biocompatibility, as evidenced by relative cell growth rates of over 90%, a hemolysis rate of below 1%, and intradermal reaction scores of less than 1.0. Importantly, it showed antithrombotic efficacy both in vitro and an in vivo. In a rabbit arterio-jugular vein shunt model, no obvious clotting was observed after 1 h of circulation. Overall, the brushes hold significant clinical potential in reducing the level of thrombus formation for blood-contacting catheters. Furthermore, by leveraging the abundant reactive amine and carboxyl groups within the p(LysAA) brushes, this approach provides a platform for integrating bioactive molecules to further mitigate the thrombotic risk.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
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IF 5.4
Wenbin Zhang,Hongzhen Cai,Xiangsheng Han,Yu Zhao,Xu Huang
Cellulose nanofibers-based membranes with balanced mechanical strength, flux and rejection rate are highly demanded in the purification of contaminated water, nevertheless, which still hard to achieve. In this work, an interfacial engineering strategy was proposed to fabricate cellulose nanofibers-based composite membranes (LC/DA@ZIF x ) using aramid nanofibers (ANFs) as reinforcing agents, lignin as interfacial binder, and 2-dimensional leaf-shaped zeolitic imidazolate framework (ZIF-L) as pore modifier. Ascribing to the dense noncovalent interactions (e.g., H-bond and π-π stacking) among each components, as well as the hierarchically layered structure, the composite membranes simultaneously exhibited promising mechanical properties (a mechanical strength of ∼163 MPa and a toughness of ∼11.89 MJ/m 3 ), flux (61.95 L·m −2 ·h −1 ·bar −1 ), and rejection rates toward contaminated water (∼97.60 % for methyl violet, 98.68 % for rhodamine B, 97.12 % for rhodamine 6 G, 93.99 % for malachite green and 98.31 % for Coomassie brilliant blue). After 10 cycles of separation, the membranes can still retain a rejection rate of ∼69.98 %. Therefore, this study provided a simple and efficient strategy for manufacturing strong cellulose-based membranes that were suitable in the fast and efficient remediation of dyestuff effluent.
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Molecular Catalysis
·
2023
·
IF 4.6
Bi-Han Guan,Wu-Tong Yin,Bo-Han Cao,Chen Zhao,Jiang Pan,Jian-He Xu
L-Carnosine (L-Car) is a biologically active dipeptide with antioxidant and anti-aging effects, formed by condensation of β-alanine (β-Ala) with L-histidine (L-His), which is used widely in the pharmaceutical and cosmetic industries. Here we report a dipeptide hydrolase originating from Bacillus megaterium , designated as Bm PepD, whose synthetic activity reached 6.5 times that of Sm PepD, which is the highest active dipeptidase reported so far for L-Car biosynthesis in vitro . By site-directed saturation mutagenesis, we obtained a significantly improved variant (T171N) of Bm PepD. It showed a specific activity of 22.9 U mg −1 protein , reaching 1.4 times that of wild-type Bm PepD or 9.1 times that of Sm PepD, which is the highest ever reported among this enzyme class. After optimizing the reaction conditions of variant Bm PepD T171N , we performed the synthetic reaction of L-Car simply from the two free amino acids (0.2 M L-His and 6.5 M β-Ala), achieving a titer of 31.3 mM L-Car with a specific productivity of 283 g Car · g catalyst −1 . This work provides a highly active and substrate (β-alanine)-tolerant dipeptide hydrolase for the facile synthesis of dipeptide L-Car via reversed hydrolysis.
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Biomed Research International
·
2017
·
IF 2.476
Li Lv,Chunxia Liu,Zhengrong Li,Fangming Song,Guocheng Li,Xingzhen Huang
The purpose of this study was to evaluate the potential of micelle to change the pharmacokinetics of quercetin (QUT), with a primary goal of enhancing its oral bioavailability. QUT-loaded methoxy poly(ethylene glycol)--poly(L-lactic acid) micelle (QUT-loaded MPEG--PLLA micelle) was prepared by a thin-film hydration method, resulting in a particle size of 88.5 nm. A liquid chromatography tandem-mass spectrometry (LC-MS/MS) method was developed and validated for determination of QUT in rat plasma. The chromatographic separation was performed on an Agilent Eclipse-C18 (4.6 mm 50 mm, 3.5 m) with an isocratic mobile phase system consisting of water and methanol (, ) at a flow rate of 0.4 mL/min. Calibration curves were linear over the concentration ranges of 2.5–2000 ng/mL for QUT. The micelle was orally administered at a single does in rats, and the pharmacokinetic parameters were evaluated and compared with that administered with the QUT aqueous suspension. The results show that the micelle was able to increase the QUT’s oral bioavailability 9-fold compared to the QUT aqueous suspension. These results suggest that methoxy poly(ethylene glycol)--poly(L-lactic acid) is a potential carrier for the oral delivery of QUT.
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MOLECULES
·
2019
·Vol. 24
·期 10
·1897
·
IF 3.06
Andreea David,Fang Wang,Xiaoming Sun,Hongna Li,Jieru Lin,Peilei Li,Gang Deng
In the present study, the composition of essential oil isolated from the roots ofVetiveria zizanioides(L.) Nash, harvested in China, was studied, along with the bioactivities. A green novel method using an eco-friendly solvent, CO2-pressurized ethanol, or carbon dioxide expanded ethanol (CXE) was employed to isolate the essential oil from the root ofVetiveria zizanioides(L.) Nash with the purpose of replacing the traditional method and supercritical fluid extraction (SFE). After investigating the major operating factors of CXE, the optimal conditions were obtained as follows: 8.4 MPa, 50 °C, 5 mL/min ethanol, and 0.22 mole fraction of CO2, presenting an extraction oil that ranged from 5.12% to 7.42%, higher than that of hydrodistillation (HD) or indirect vapor distillation (IVD). The Gas Chromatography-Mass Spectrometry (GC-MS) analysis showed that three major components, including valerenol (18.48%), valerenal (10.21%), and β-Cadinene (6.23%), are found in CXE oil, while a total of 23 components were identified, 48 components less than using conventional hydrodistillation. Furthermore, the antimicrobial activities of root oils were evaluated by the microdilution method, which showed that CXE oil exhibited an ability against Gram-positive bacteria, especiallyStaphylococcus aureus, approximately equivalent to traditional samples. Additionally, the DPPH free radical scavenging assay demonstrated that the antioxidant abilities of root oils were sorted in the descending order: IVD > HD > CXE > SFE. In conclusion, after a comprehensive comparison with the conventional methods, the CXE-related technique might be a promising green manufacturing pattern for the production of quality vetiver oil, due to the modification of ethanol by the variable addition of non-polar compressible CO2, ultimately resulting in a prominent dissolving capability for the extraction of vetiver solutes.Keywords:vetiver essential oil;CO2expanded ethanol;hydrodistillation;chemical composition;antimicrobial activity;DPPH free radical scavenging assay
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JOURNAL OF MOLECULAR LIQUIDS
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2020
·
IF 5.065
Lian Yu,Yao Meng,Ze-Lin Wang,Liu Cao,Chang Liu,Ming-Zhu Gao,Chun-Jian Zhao,Yu-Jie Fu
Nowadays, developed an efficient and sustainable method to extract bioactive compounds from plants biomass has gained more and more attention. As surfactant aqueous possesses an obviously advantage of highly extraction efficiency from the raw plants, it is naturally to emerge as an alternative to traditional extractant. In order to obtain the maximum yield in the shortest time, the surfactant-based microwave-assisted extraction was applied to extract five target bioactive compounds from fig ( Ficus carica L.) leaves. Under optimal conditions, the extraction yields of caffeoylmalic acid, psoralic acid-glucoside, rutin , psoralen and bergapten reached 9.72 mg/g, 5.95 mg/g, 4.77 mg/g, 15.65 mg/g and 3.49 mg/g, respectively. These results showed that the final extraction yields were much higher than those by traditional methods. The present study indicated that the surfactant (PEG8000)-based microwave-assisted extraction method was extremely sustainable and efficient for extraction of active ingredient from fig leaves.
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Nutrients
·
2025
·Vol. 17
·期 15
·2456
·
IF 5
Ming Chu,Yingying Tong,Lei Zhang,Yu Zhang,Jun Dang,Gang Li
Background: The metabolic dysfunction-associated steatotic liver disease (MASLD) represents an escalating global health concern, with effective treatments still lacking. Given its complex pathogenesis, multi-targeted strategies are highly desirable. Methods: This study reports the isolation of four flavone C-glycosides (FCGs) from Dianthus superbus L. and explores their potential in treating MASLD. The bioactivity and underlying mechanisms of FCGs were systematically evaluated by integrating network pharmacology, molecular docking, and zebrafish model validation. Results: Network pharmacology analysis revealed that FCGs may modulate multiple MASLD-related pathways, including lipid metabolism, insulin signaling, inflammation, and apoptosis. Molecular docking further confirmed strong binding affinities between FCGs and key protein targets involved in these pathways. In the zebrafish model of MASLD induced by egg yolk powder, FCGs administration markedly attenuated obesity, hepatic lipid accumulation, and liver tissue damage. Furthermore, FCGs improved lipid metabolism and restored locomotor function. Molecular analyses confirmed that FCGs upregulated PPARγ expression to promote lipid metabolism, restored insulin signaling by enhancing INSR, PI3K, and AKT expression, and suppressed inflammation by downregulating TNF, IL-6 and NF-κB. Additionally, FCGs inhibited hepatocyte apoptosis by elevating the BCL-2/BAX ratio. Conclusions: These findings highlight the multi-pathway regulatory effects of FCGs in MASLD, underscoring its potential as a novel therapeutic candidate for further preclinical development.
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