TALANTA
·
2019
·
IF 4.916
Muhammad Muhammad,Guohua Yao,Jie Zhong,Kuanglin Chao,Muhammad Hammad Aziz,Qing Huang
Fipronil is a phenylpyrazole insecticide commonly used in agriculture and residential applications. In this paper, we reported a novel label-free surface-enhanced Raman spectroscopy (SERS) method for detection of fipronil residues in chicken eggs (mostly accumulated on the egg membrane). We fabricated the SERS substrates composed of the SiO 2 @Au core/shell nanoparticles and probed the contamination of fipronil residue on the egg membrane. The identification of the characteristic Raman bands of fipronil was achieved with the aid of density functional theory (DFT) calculation, with which we could analyzed the trace amount of fipronil in a quantitative way. As such, this work may provide a practical solution to quick inspection of fipronil contamination in chicken eggs or other foods.
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DOI
高影响因子
METHODS
·
2025
·
IF 4.3
Biqing Chen,Jiayin Gao,Haizhu Sun,Yinghan Zhao,Yan Liu,Xiaohong Qiu
Traditional surface-enhanced Raman scattering (SERS) technology often struggles to achieve high-precision discrimination in the simultaneous detection of multiple exogenous hormones due to complex spectral overlap and matrix interference, limiting its application in trace analyte analysis within complex matrices (e.g., biological samples). This study developed a SERS substrate based on carboxyl-terminal polyethylene glycol (PEG)-modified iron oxide (Fe 3 O 4 ) nanoparticles (Fe 3 O 4 @PEG), integrated with artificial intelligence (AI)-driven high-throughput spectral analysis algorithms. This approach successfully enabled ultrasensitive detection and precise discrimination of multiple typical exogenous hormonal drugs, including tamoxifen, drospirenone, cyproterone acetate, medroxyprogesterone acetate, estradiol ester derivatives, and dydrogesterone. By optimizing the surface enhancement effect of Fe 3 O 4 @PEG nanocomposites and employing machine learning models (e.g., convolutional neural networks, CNN) for collaborative analysis, the weak Raman fingerprint features of target hormones in complex mixtures were effectively extracted and classified, achieving a detection limit at the corresponding to 10 −7 –10 −8 mg/mL level. In matrix-spiked serum and urine samples, which mimic complex biological matrices validations, the AI-SERS platform demonstrated exceptional performance in the identification and quantitative analysis of target exogenous hormones. This research provides an intelligent analytical strategy for rapid and highly sensitive detection of multiple trace exogenous hormones in complex matrices.
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DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Xuan Fu,Limei Yin,Yang Zhang,Zongbao Sun,Shanshan Xue,Heera Jayan,Hesham R. El-Seedi,Xiaobo Zou,Zhiming Guo
Aflatoxin B1 (AFB1) is a typical toxin found in maize, leaving severe threats to human health and livestock. A dual-signal SERS-fluorescence aptasensor was constructed to detect Aflatoxin B1. Hollow mesoporous silica nanoparticles functionalized with amino groups (AHMSN) were designed as carriers for the dual-signal molecule Rh6G. The aptamer, adsorbed onto the surface of AHMSN Rh6G via electrostatic interactions, acted as a “gate” to block the release of Rh6G. Simultaneously, the noble metal nanocomposite Au@Ag NRs was developed as the SERS substrate. The strong and selective interaction between the aptamer and AFB1 led to the release of Rh6G, resulting in SERS and fluorescence signals that were positively correlated with the concentration of AFB1. The LOD of SERS and fluorescence were 0.0092 ng/mL and 0.0050 ng/mL, respectively. At the same time, the sensor demonstrated good accuracy in detecting AFB1 in various maize meal/oil samples, showing strong practical applicability. This research developed a highly specific and sensitive dual-mode detection method for AFB1, circumventing the need for aptamer chemical modification or the incorporation of complementary strands. This approach presents a simplified, cost-efficient, and versatile strategy for AFB1 detection.
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产品关联
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2025
·
IF 6.9
Xiaoyi Rong,Shaojie Gong,Jinqing You,Lin Geng,Wenzhong Yang,Hui Xu
Surface-enhanced Raman scattering (SERS) stands out as a valuable tool for the rapid and precise detection of trace antibiotics. However, preparing SERS substrates with high sensitivity, homogeneity, and good stability remains a great challenge. In this study, we chemically functionalized graphene oxide (GO) with poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) through surface-initiated atom transfer radical polymerization (SI-ATRP) technique. Then, GO- g - q PDMAEMA was obtained by quaternization (the quaternized PDMAEMA is denoted as q PDMAEMA). Using a water/oil interface self-assembly approach, we fabricated GO- g - q PDMAEMA/AuNPs nanofilms by interfacial assembly between GO- g - q PDMAEMA in chloroform and AuNPs in water. The nanofilms exhibited remarkable SERS characteristics, including exceptional sensitivity, good homogeneity, reproducibility, and stability. Additionally, two commonly used antibiotics, i.e., sulfamonomethoxine (SMM) and enrofloxacin (ENR) were rapidly and sensitively detected using the prepared GO- g - q PDMAEMA/AuNPs nanofilms as a SERS platform. The developed GO- g - q PDMAEMA/AuNPs nanofilms hold significant promise for food safety monitoring and environmental analysis.
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高影响因子
TALANTA
·
2025
·
IF 6.1
Shuojiang Liu,Ying Chen,Xingliang Cheng,Gongke Li,Yuling Hu
Surface-enhanced Raman scattering (SERS) in conjunction with nanozymes has emerged as a powerful technique for bioanalysis due to their signal conversion and amplification effect. However, clinical applications continue to encounter challenges such as inadequate selectivity. Herein, we proposed a novel approach for the selective SERS detection of L-DOPA in human serum with dual enzyme-mimicking MOF-919(Fe–Cu). This material exhibits both catechol oxidase-like and peroxidase-like activities, enabling it to catalyze catecholic compounds to o -quinones and subsequently oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) molecules into SERS-active ox-TMB. Ultrasonic-assisted synthesis ensures uniform and dense loading of AgNPs within MOF-919(Fe–Cu), thereby enhancing SERS activity. A sensitive and selective method for SERS detection of L-DOPA was developed. The catechol oxidase-like activity of MOF-919(Fe–Cu) provides ideal selectivity and anti-interference to L-DOPA, while the cascade catalytic system transforms weak SERS signals of L-DOPA into amplified SERS signals of ox-TMB. The mechanism of dual enzyme-mimicking activity was further discussed. The detection process was optimized to achieve high sensitivity towards L-DOPA with a linear range 0.5–100 μmol/L and low limit of detection (LOD) of 0.16 μmol/L. Furthermore, it has been applied in serum sample of Parkinson's patients, demonstrating excellent recovery and low relative error compared to HPLC-UV. This approach offers an economical, efficient and accurate strategy for the detection of L-DOPA and provides valuable insights for the integration of selective multifunctional nanozyme and sensitive SERS detection method.
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Rongyang Liu,Li Jiang,Zizhen Yu,Xufeng Jing,Xiu Liang,Dan Wang,Bo Yang,Chengxing Lu,Wei Zhou,Shangzhong Jin
Recent years, two-dimensional transition metal carbonitrides, MXene, have emerged as SERS substrates due to their unique optical and electrical properties. In order to combine the advantages of metal nanoparticles and MXene for SERS substrates, this paper prepared an MXene (Ti 3 C 2 T x ) -Ag nanoparticles (NPs) hybrid biosensor through in-situ electrostatic self-assembly method, in which the citrate-coated Ag NPs are loaded on the negatively surface of Ti 3 C 2 T x flakes through positively charged PDDA polymer. The characterizations show that a large number of Ag NPs uniformly adsorb on both side of Ti 3 C 2 T x nanosheets. The prepared platform exhibits excellent SERS performance, long-term stability and good uniformity by detecting 4-MBA molecules. The electromagnetic field distribution was simulated to theoretically show the Raman enhancement mechanism of this platform. In order to explore the possible application of the sensor in clinical diagnosis and monitoring of biological environment, the platform was used for biomolecule detection. The detection limit of adenine molecules can be as low as 10 −8 M. For dopamine molecules with concentration ranging from 5 × 10 -6 M to 5 × 10 −8 M, the SERS results show a linear relationship between peak intensity and concentration of dopamine molecules. The biosensor was applied for serum detection. The results suggest that MXene-PDDA-Ag NPs hybrid platform could be used as a sensitive and uniform biosensor for label-free quantitative detection of bio-molecular based on SERS method, which could be applied in the field of biotechnology.
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高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2011
·
IF 3.068
Jingpeng Yuan,Yongchao Lai,Junling Duan,Quanqin Zhao,Jinhua Zhan
A mono-6-thio-β-cyclodextrin-modified silver film was synthesized via galvanic displacement on copper foil. The prepared silver films could enrich non-polar polychlorinated biphenyls (PCBs) molecules from hydrophilic phase using thiolate β-cyclodextrins (SH-β-CDs) as receptors. The components of as-prepared Ag-coated-Cu (Ag–Cu) film were confirmed by powder X-ray diffraction (XRD). Both surface-enhanced Raman spectroscopy (SERS) and energy dispersive X-ray spectroscopy (EDS) measurements gave strong evidences that the thiolated β-cyclodextrins (SH-β-CDs) had been immobilized on the surface of silver film. Compared to the substrates prepared in the absence of SH-β-CD, the surface morphology of the CD-modified Ag films was obviously changed. The interfacial enrichment and the capability of substrates to form inclusion complexes with PCBs molecules were tested by using PCB-15 (4,4′-dichlorobiphenyl) as the probe molecules via SERS technique. The measured SERS spectra could distinguish the PCB-15 molecules at micro-molar level according to the most intense CCC bending in-plane mode of PCBs. The enhancement factor (EF) of the SERS substrates for PCB-15 was 1.2 × 10 5 , which was comparable with a number of previous reports.
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高影响因子
ACS Applied Bio Materials
·
2021
·
IF 0
Menghui Wan,Haodong Zhao,Lichao Peng,Yanbao Zhao,Lei Sun
The fabrication of highly active and free-standing surface-enhanced Raman scattering (SERS) substrates in a simple and low-cost manner has been a crucial and urgent challenge in recent years. Herein, SiO2 nanofiber substrates modified with size-tunable Ag nanoparticles were prepared by the combination of electrospinning and in situ chemical reduction. The results demonstrate the presence and uniform adsorption of Ag nanoparticles on the SiO2 matrix surface. The free-standing composite nanofibrous substrates show high-performance SERS response toward 4-mercaptophenol and 4-mercaptobenzoic acid, and the detection limit can be as low as 10–10 mol/L. Most importantly, the as-prepared substrate as a versatile SERS platform can realize label-free detection of bio-macromolecules of bacteria, i.e., Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). Furthermore, the substrates also possess outstanding antibacterial activities against S. aureus and E. coli. Briefly, the significance of this study is that size-tunable Ag nanoparticles can be decorated on SiO2 nanofiber surfaces with triethanolamine as a bridging and reducing agent through a one-pot reaction, and the as-prepared nanofibrous membranes are expected to act as a candidate for label-free SERS detection as well as antibacterial dressing.
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DOI
JOURNAL OF RAMAN SPECTROSCOPY
·
2019
·Vol. 50
·期 11
·1679-1690
·
IF 2.809
Hang Zhao,Wuliji Hasi,Nan Li,Xuanyu Sha,Siqingaowa Han
This paper reports a “bottom-up” substrate preparation method using a two-phase interface self-assembly technology that combines silver-coated gold core–shell nanorods with the thermally shrinkable polystyrene (TSP) support material for surface-enhanced Raman spectroscopy (SERS), that is, TSP-SERS substrate. The gold nanorods with long absorption wavelength were used as the core, and the silver shells were coated to obtain the core–shell nanostructures with a stronger resonance with the wavelength of the light source. The density of the nanostructures and numbers of “hot spots” within the light spot increased via the three-dimensional folding feature formed by thermal shrinkage. The combined effect of the two factors increases the enhancement factor by an order of magnitude to 10 7 after thermal contraction. The detection concentration of 4-mercaptobenzoic acid can reach 10 −9 M, and the maximum relative standard deviation is only 8.9%. In fact, the detection limit of benzimidazole on the surface of apple can reach 0.5 mg/L, and the recovery deviation is controlled within the range of 11.7%. The practical detection of benzimidazole pesticide residues showed that this method has wide application prospects in the detection of pesticide residues.
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高影响因子
Nanoscale
·
2017
·Vol. 9
·期 25
·8879-8888
·
IF 7.367
Xiu Liang,Yi-Shuo Wang,Ting-Ting You,Xiao-Juan Zhang,Nan Yang,Guang-Sheng Wang,Peng-Gang Yin
Interfacial self-assembly of ordered nanostructures at oil–water interfaces towards the fabrication of nanofilms has attracted the interest of plenty of scientists, since its discovery in 2004. Herein, further developments have been achieved, and we report a new strategy for the synthesis of a three-dimensional (3D) hierarchical nanostructure, through an interfacial synthesis driven microemulsion process. Thus, the synthesis route has been simplified, with the rigorous experimental conditions of traditional compositing technology. Combined with a two-step seed-mediated growth method for preparing uniform Ag-NPs, a plasmonic 3D MoS2-NS@Ag-NP nanostructure was successfully developed as a Surface-Enhanced Raman Scattering (SERS) active substrate, with plenty of surface hot spots, leading to an enhancement factor (EF) of 1.2 × 108 derived from both electromagnetic mechanism (EM) and chemical mechanism (CM) effects. The 3D MoS2-NS@Ag-NP nanostructure can be applied to detect trace thiram in apple juice and local lake water, with a detection limit as low as 10 ppb (42 nM), which is much lower than the maximal residue limit (MRL) of 7 ppm in fruit prescribed by the U.S. Environmental Protection Agency (EPA). Furthermore, quantitative analysis was achieved in the range of 10 ppb–1 ppm with good homogeneity and selectivity.
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DOI
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 191
·期 1
·1-10
·
IF 5.7
Chen Jiawei,Zeng Yan,Zhang Dun,Qi Peng,Liu Xuguang,Song Ruiguo,Wang Peng
A direct and ultra-sensitive surface-enhanced Raman scattering (SERS) immunoassay method is introduced for the detection of Escherichia coli and Staphylococcus aureus . This methodology is based on a sandwich-structured complex probe (SCP) mechanism, combined with target-induced strand displacement. Moreover, by leveraging the amplified SERS signal from gold nanoparticles (AuNPs) corresponding to an increase in bacterial count, we achieve quantitative determination. The SCP demonstrates remarkable specificity, sensitivity, and anti-interference capability in bacterial detection. The detection limits for both bacterial strains are as low as 10 CFU/mL. In our selectivity tests, all peak intensities had standard deviations ( n = 3) below 6%. Recoveries in normal human serum were 101–110% for E. coli and 96–101% for S. aureus . In milk, the recoveries were 102–105% for E. coli and 100–105% for S. aureus , respectively, demonstrating a high level of accuracy and resistance to interference. In addition, the SCP offers a dual-detection capability, enabling simultaneous diagnosis of multiple targets, which greatly simplifies the testing procedure. The findings underscore that this immunoassay platform fulfills the demand for rapid and precise pathogenic bacterial diagnosis, holding substantial potential for practical applications. Graphical abstract
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Linlin Chen,Qunlong Wang,Yuting Jing,Ruijing Wang,Xuefeng Wang
Excessive food pigments can be harmful to human health and in severe cases can cause cancer. Unlike conventional and time-consuming detection techniques, surface enhanced Raman scattering (SERS) is a fast, sensitive and accurate method. Ag NPs@NiO foam is developed as an efficient SERS substrate, featuring a large specific surface area and suitable charge transfer pathways. R6G can be detected at a concentration as low as 10 −14 M with an analytical enhancement factor (AEF) as high as 1.682 × 10 12 , demonstrating the high sensitivity of Ag NPs@NiO foam. In practical applications, Ag NPs@NiO foam is successfully used for the detection of food blue, amaranth, allura red in beverages, with the limit of detection lower than the safety limits in China. This facile and highly sensitive substrate provides a solid foundation for fast and accurate food analysis.
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）