JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
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2025
·Vol. 36
·期 25
·1-13
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IF 2.8
Zhang Yuexing,Feng Wenlin,Ullah Arif
To address the need for high-sensitivity carbon monoxide (CO) gas sensing at room temperature, a CO gas sensor based on Au-modified tin oxide/zinc oxide (SnO₂/ZnO) nanocomposites is fabricated. SnO₂ nanoparticles, ZnO nanosheets, and Au nanoparticles (AuNPs) were synthesized using sacrificial template, hydrothermal, and chemical reduction methods, respectively. The resulting nanocomposite was then drop-casted onto interdigitated electrodes and dried to obtain the CO gas sensor. The performance of the sensor was evaluated using a custom airtight chamber and a semiconductor parameter analyzer. The n–n heterojunction between SnO₂ and ZnO significantly increased oxygen vacancy in the composite material, enhancing surface chemisorption of oxygen. Moreover, the catalytic sensitization effect of AuNPs facilitated electron migration, while their inherent spillover effect promoted the activation of gas molecule interactions. Consequently, the sensor exhibited a high response (22.11%) to 70 ppm CO at room temperature, along with excellent stability, selectivity, and anti-interference capabilities. This work offers a promising approach for developing highly sensitive room-temperature gas sensors.
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Virology Journal
·
2025
·Vol. 22
·期 1
·1-11
·
IF 3.8
Zeng Huiran,Wang Yuan,Tian Yachen,Yao Xiaoting,Li Yixuan,Xu Linan,Yang Xiangpeng,Zhang Xinglin,Ma Junfei
The high-level colonization of vancomycin-resistant Enterococci (VRE) in the gastrointestinal tract could lead to systemic infections such as bacteremia, endocarditis, and urinary tract infections, particularly in hospitalized patients. Given the potent bactericidal activity and host specificity of bacteriophages, phage therapy represents a promising alternative strategy for controlling VRE infections. In this study, we isolated and characterized phage A155, which targets vancomycin-resistant Enterococcus faecalis (VR-Efs) V583. Genomic analyses revealed that it is a member of the Kochikohdavirus genus, while functional characterization defined its optimal multiplicity of infection (MOI), one-step growth kinetics, and stability under varying thermal (20–50 °C) and pH (3.0–11.0) conditions. The phage demonstrated a broad lytic spectrum and effective in vitro antibacterial activity. Furthermore, phage A155 could significantly reduce the VRE intestinal colonization loads by 1.13 orders of magnitude in a mouse model. These findings exhibit the characterization and genome analysis of a novel VR-Efs phage A155, highlighting its therapeutic potential against E. faecalis colonization.
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Journal of Translational Medicine
·
2025
·
IF 7.5
Liuzhu Chen,Linpei Zhuo,Jie Zheng,Xiaoyun Sun,Jing Sun,Jiaming Liu
Background Recent studies have highlighted the vital role of gut microbiota in the pathogenesis of Ischemic stroke (IS). However, the effects and underlying mechanisms of atorvastatin on IS via regulating gut-brain axis remain unclear. Thus, this study aimed to explore the relationship between atorvastatin, gut microbiota and IS through animal experiments, clinical trials and Mendelian randomization (MR) analysis. Methods Male mice were induced with bilateral common carotid artery occlusion (BCCAO) to establish an IS animal model, and then intragastrically treated with atorvastatin. Neurological deficits, microglia activation, and the levels of NLRP3 inflammasome and NF-κB pathway-related proteins were detected. Meanwhile, gut microbiota composition and intestinal barrier integrity were evaluated. In this prospective study, we recruited IS patients undergoing atorvastatin treatment, evaluated their functional outcomes, collected fecal samples, and assessed gut microbiota functions. Moreover, the causal relationships between specific bacteria, inflammation and IS were assessed via MR analysis. Results Our results showed that atorvastatin treatment significantly improved neurobehavioral deficits, suppressed activation of microglia, and inhibited NF-κB pathway as well as the formation of the NLRP3 inflammasome, reduced the release of inflammatory cytokines, including IL-1β and IL-18, which were reversed by antibiotics treatment. We further identified an increase in the genus Lachnospiraceae NK4A136 in atorvastatin-treated mice. Subsequent clinical experiments were conducted to explore the effects by analyzing the characteristic bacteria, such as Ruminococcus torques and Lachnospiraceae NK4A136 . The higher abundances of Ruminococcus torques and Lachnospiraceae NK4A136 were associated with a good outcome in atorvastatin-treated IS patients. MR analysis further revealed that these microbes were negatively correlated with inflammatory factor levels, and showed inhibitory effects on the Akt/NF-κB/NLRP3 pathway and PLA2G7 gene expression. Conclusion These findings demonstrated the roles of atorvastatin in regulating Akt/NF-κB/NLRP3 pathway to inhibit neuroinflammation through specific bacteria, which implied a novel way for IS treatment. Supplementary Information The online version contains supplementary material available at 10.1186/s12967-025-06803-w.
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JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
·
2025
·
IF 3.2
Wang Haitao,Liu Xiang,Gu Xin,Wang Yao,Wang Zhenyu,Chen Zihao,Liang Xiaolu,Luo Yuping,Tan Songlin
Y 2 O 3 : MgO (YMO) composite materials exhibit outstanding optical and thermal stability, and are widely used in optics, catalysis, ceramics and other fields. Attaining high-performance YMO materials hinges upon the use of high purity finely ground raw materials, precise component ratios, and uniform distribution. In this study, YMO composite nanoparticles with a volume ratio of 50:50 were synthesized using oxidizer (magnesium nitrate hexahydrate and yttrium nitrate hexahydrate) and fuel (citric acid). Optimizing the particle size and specific surface area of the nanoparticle was achieved by adjusting the oxidizer to fuel ratio ( O/F ) in the precursor (the molar ratio ranges from 0.18 to 0.33), and the optimal O/F was obtained. When the molar ratio is at 0.28, nanoparticles with particle size of 14 nm and specific surface area of 44 m 2 /g are synthesized. Furthermore, this study revealed that nanoparticles synthesized at different molar ratios of O/F produce different agglomeration degree after calcination at 800 °C, with the agglomeration factor (AF) gradually decreasing as the molar ratio of citric acid to nitrate increases; the results show that AF is at least 2.0 when O/F = 0.28.
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BMC Medicine
·
2025
·Vol. 23
·期 1
·1-14
·
IF 8.3
Cheng Xin,Wang Zerui,Liu Yadong,Ma Liyan,Wang Zhiyi,Qiao Yuxin,Du Wenbin,Guo Shuilong,Chen Dongwei,Su Jianrong
Background Heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) is associated with suboptimal glycopeptide treatment outcomes. However, conventional antimicrobial susceptibility testing fails to distinguish hVISA from vancomycin-susceptible Staphylococcus aureus (VSSA), potentially delaying effective therapy. The gold standard population analysis profiling with area under the curve (PAP-AUC) method is labor-intensive and time-consuming, necessitating rapid detection alternatives. Methods This study introduces a microfluidic platform based on fluorescence-activated droplet sorting (FADS) for the rapid detection and isolation of hVISA. Through systematic optimization of key parameters such as droplet generation conditions, bacterial suspension concentration, fluorescence probe selection, fluorescence duration, and vancomycin screening concentration, we established a rapid hVISA screening system. The efficacy of this system was evaluated using the hVISA standard strain Mu3, followed by validation with 15 clinical hVISA isolates. Genomic analysis further elucidated the genetic basis of drug resistance in hVISA strains. Results We developed a high-throughput detection platform for hVISA by integrating a microfluidic system with FADS. Based on a Poisson distribution theoretical model, the bacterial suspension concentration was optimized to 10 6 CFU/mL, achieving a single-bacterium droplet encapsulation rate of approximately 30%. The selected fluorescent probe exhibited detectable fluorescence signals as early as 6 h post-cultivation, with stability maintained for at least 1 week. Drug concentration validation demonstrated that 4 μg/mL vancomycin effectively inhibited susceptible bacteria without interfering with fluorescence detection. This platform successfully isolated resistant subpopulations from the hVISA reference strain Mu3 within 12 h, and its efficacy was further validated using 15 clinical hVISA isolates. Conclusions This study innovatively integrated a microfluidic detection platform and FADS to achieve rapid detection of hVISA. This technology reduced the detection time to just 12 h and significantly improved the enrichment efficiency of drug-resistant bacterial populations.
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JOURNAL OF THROMBOSIS AND THROMBOLYSIS
·
2025
·
IF 2.2
Lin Shan,Li Yingjie,Zhang Yue,Wang Qi,Qu Xinrui,Wang Na,Zhong Xinrui,Zhang Shuang,Zhang Daiyan,Wang Zeyu,Li Sen
Atherosclerosis is the main cause of cardiovascular diseases. These diseases can lead to mortality and morbidity worldwide. Macrophage apoptosis plays an important role in the progression of atherosclerosis. ASA-X 3 has anticancer effects by inducing significant apoptosis in gastric cancer cells. We conducted a preliminary experiment and reported that ASA-X 3 promotes macrophage apoptosis. Therefore, we hypothesize that ASA-X 3 might play a role in anti-atherosclerosis by inducing macrophage apoptosis. The effects of ASA-X 3 and ASA on RAW264.7 cells (in vitro) and mice (in vivo) were investigated. In vitro, RAW264.7 cell viability and apoptosis rates were assessed, and the levels of PARP, caspase-9, p-JNK, and p53 in cells treated with the two substances were compared. In vivo, Terminal-deoxynucleoitidyl Transferase Mediated Nick End Labeling (TUNEL) assays revealed macrophage apoptosis in mice treated with medium- or high-dose ASA-X 3 or ASA. Aortic plaques in mice treated with either drug were examined using oil red O and H&E staining. Masson and collagen staining was performed on the aortic rings of mice treated with high-dose ASA-X 3 or ASA. In vitro, unlike ASA, which had no effect, ASA-X 3 markedly reduced RAW264.7 cell viability. ASA-X 3 induced apoptosis at a rate about four times higher than ASA, with increased levels of PARP, caspase-9, phosphorylated JNK, and p53. In vivo, medium- and high-dose ASA-X 3 significantly increased macrophage apoptosis in mice compared with ASA. In addition, ASA-X 3 (at medium and high doses) reduced the number of aortic plaques, as shown by oil red O and H&E staining. Masson and collagen staining of aortic rings revealed similar results for the high-dose ASA-X 3 and ASA treatments. ASA-X 3 appeared to exhibit anti-atherosclerosis effects via a mechanism that involves the induction of macrophage apoptosis and the lowering of blood plasma lipid levels, eventually resulting in reduced aortic plaque. ASA-X 3 could, therefore, be considered a promising candidate for the treatment of this cardiovascular disease. Graphical
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JOURNAL OF MATERIALS SCIENCE
·
2025
·
IF 3.9
Zhao Xueying,Tang Peng,Wu Guohua,Li Mang,Du Jiawei,Zhang Xuyang,Liu Nana,Wang Bo,Zhang Yaohong,Wang Xiangwei
Despite its high conductivity and lightweight properties, carbon black (CB) suffers from insufficient electromagnetic wave (EMW) dissipation and severe particle agglomeration, limiting its performance in advanced microwave absorption applications. To overcome these challenges, we developed a methylammonium lead iodide perovskite (MAPbI 3 )/CB composite system featuring micron-structured MAPbI 3 frameworks via a simple milling process. The micrometer-scale MAPbI 3 acts as a scaffold, effectively suppressing CB aggregation while simultaneously optimizing dielectric loss and impedance matching. This synergistic design enhances microwave absorption performance by improving EMW attenuation and broadening the effective absorption bandwidth (EAB). At a filling mass of 24 wt.%, the MCB-16/8 composite with CB fixed at 8 wt.% and MAPbI 3 share of 16 wt.% exhibits excellent EAB absorption properties, with an EAB reaching a wide absorption of 5.44 GHz at 2.05 mm and a minimum reflection loss as low as −64.22 dB at 2.3 mm. Also, the simulation results show the power loss density distribution within the MCB-16/8 and confirm a significant reduction of the radar cross-section (RCS), with excellent agreement between simulation and experimental data. This study presents a novel approach to address the agglomeration of carbon-based materials by constructing a micron-nanometer step heterostructure, offering an effective pathway for developing high-performance EMW absorption composites. Graphical
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MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 12
·1-9
·
IF 6.232
Zhao Yunfei,Chen Jun,Zhong Hangtian,Zhang Chengli,Zhou Yuan,Mao Weiran,Yu Chao
The development of a novel signal amplification system is described for sensitive determination of α2,6-sialylated glycans (α2,6-sial-Gs), an important prognostic tumor biomarker. First, Fe-based metal-organic frameworks (Fe-MOFs) with silver nanoparticles (AgNPs) decorated onto the outer surface were designed and synthesized with controlled octahedron structures. The new Ag/Fe-MOFs nanocomposite possessed strong conductivity and a large surface area to carry more nanoprobes. To connect the Ag/Fe-MOFs nanocomposite with more groups, the nanocomposite was functionalized by -COOH with SH-PEG-COOH to bind with an α2,6-sial-Gs catcher, M-APBA, via -CONH- bonds. More importantly, the Ag/Fe-MOFs also exhibited an excellent endogenous redox mediator property to produce electrons, which is the fundamental mechanism underlying amplification of an electronic signal. A gold electrode was used to accelerate electron transfer and immobilize the α2,6-sial-Gs lectin (SNA). After the sandwich-type catcher recognition (SNA/α2,6-sial-Gs/M-APBA), the current peak response was provoked in the process of oxidizing AgNPs to Ag + in the forward anodic potential sweep, while Cl − in a PBS solution was transferred into Ag + to maintain charge neutrality. Optimized particles were employed for direct fabrication of the sandwich-type affinity biosensor, which was found to show a linear detection range from 1 fg mL −1 to 1 ng mL −1 with a detection limit of 0.09 fg mL −1 . Furthermore, the biosensor exhibited excellent specificity and stability, indicating that such a novel nanobiotechnology platform can be used to initiate potential utility for monitoring biomarkers in serum. Graphical abstract
(A)Schematic presentation of synthesis and surface modification of Ag/Fe-MOFs. The new Ag/Fe-MOFs nanocomposite possessed commendable conductivity and large surface area to carry more nanoprobe; after functionalizing the Ag/MOFs with SH-PEG-COOH, the functionalized endogenous redox mediator (c-Ag/MOFs) realized the possibility that can connect with the biological catcher. (B) Schematic diagram of electrode construction for detecting α2,6-sialylated glycans (α2,6-sial-Gs). By using the c-Ag/Fe-MOFs functional endogenous redox mediator, we successfully implemented the electrochemical detection of α2,6-sial-Gs.
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International Journal of Environmental Research
·
2024
·Vol. 18
·期 5
·1-11
·
IF 2.6
Yu Ying,Kang Wenhui,Song Jianan,He Zhenli,Wang Jianguo,Liu Yu,Yan Zhiyu
Thermally-enhanced soil vapor extraction (T-SVE) combined with sand mixing is an alternative technology for remediating low-permeable soil polluted by organic contaminants. In this study, a T-SVE apparatus with a large heating cylinder was constructed for exploring removal mechanisms of typical petroleum hydrocarbons of n -C 11 and C 13−16 alkanes, and dynamics of heat propagation within soils during T-SVE operation was simulated by CMG-STARS software. After 6 days of T-SVE, most of the soil concentration-gradient curves of n -alkanes almost coincided with their isothermal contours, suggesting the crucial role of heat conductivity on T-SVE remediation efficiency. The instantaneous concentration of n -alkanes in extracted gas showed great fluctuations, and higher than 90% of spiked n -alkanes was removed from soils. n -alkanes were more easily eliminated from soil with lower organic matter due to the less retention ratio despite the trivial impact of organic matter on heat transfer. By contrast, the higher soil moisture adopted in the study retarded heat transfer in the initial 2 days due to thermal consumption induced by water evaporation. During the last 4 days, however, the average temperature of more humid soil was obviously elevated, especially in the zones receiving less energy input. It was attributed to the fact that soil temperature was mainly determined by heat conduction, and soil pores still occupied by water were more beneficial for heat conduction, leading to the promoted evaporation of n -alkane and therefore eventual remediation efficiency. Suitable original soil moisture is important for T-SVE remediating low-permeable soil combined with sand mixing.
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JOURNAL OF MATERIALS SCIENCE
·
2024
·
IF 3.5
Yu Fengqin,Xie Qiang,Zhang Zhijun,Yang Shuai,Liu Jinchang,Ren Qiang,Liang Dingcheng
Zeolites are widely used for removing volatile organic compounds (VOCs) in industrial processes. However, the understanding of the competitive adsorption behavior of multicomponent VOCs on zeolites remains insufficient. This study employed grand canonical Monte Carlo simulation to investigate the adsorption behaviors of nonpolar benzene and polar ethyl acetate in both pure and mixed forms on FAU, LTA and MFI zeolites. The effects of zeolite topology, Si/Al ratio, and compensating cations on competitive adsorption were explored. The results revealed that the adsorption capacities of benzene and ethyl acetate on FAU and LTA zeolites gradually increased with increasing Si/Al ratio, consistent with the increasing trends in their specific surface areas and pore volumes. However, the MFI zeolite shows no significant change in these properties with varying Si/Al ratios. Zeolites modified with Ca 2+ significantly improved the benzene and ethyl acetate adsorption capacities, while impact on ethyl acetate adsorption is minimal. Zeolites modified with K + reduced the adsorption capacities for both benzene and ethyl acetate. For binary mixtures, Na-FAU-1, Na-LTA-1, and Na-MFI-15 exhibited high selectivity for ethyl acetate. Increasing the Si/Al ratio and K + -modification reduced the adsorption selectivity of these zeolites for ethyl acetate. Ca 2+ -modification reduces the selectivity of LTA-1 toward ethyl acetate, with minimal impact on FAU-1 and MFI-15. Dynamic adsorption experiments with commercial zeolites confirm the reliability of the simulation results in predicting benzene and ethyl acetate adsorption performance. This study is expected to provide guidance for selecting appropriate zeolites for VOCs mixtures in practical applications. Graphical abstract
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Scientific Reports
·
2025
·Vol. 15
·期 1
·1-13
·
IF 3.9
Dong Yao,Zhou Jiazhi,Tian Hairui,Gai Zhonghui,Zou Kang,Wei Quanwei,Han Mei
This study aimed to investigate the effects of probiotic Bifidobacterium longum subsp. longum BL21 on reproductive health in zebrafish ( Danio rerio ), focusing on hormonal modulation, sperm vitality, and overall reproductive function improvement. Adult male zebrafish of the wild-type AB strain were divided into four groups: control (CTL), model control with cyclophosphamide-induced oligospermia (CS), probiotic-treated (BL21), and a positive control treated with clomiphene citrate (CC). All groups, except CTL, were exposed to cyclophosphamide from day 1 to day 9 to induce reproductive dysfunction, with subsequent monitoring until day 16. Key metrics assessed included body and testicular weight, sperm vitality, male courtship behavior, hormone levels, sperm DNA fragmentation, and gene expression of PCNA, NANOG, ZBTB16, mTOR, DDX4, CYP26A1, and ALDH1A2. In this study, BL21 demonstrated significant therapeutic potential in treating reproductive dysfunction in zebrafish. Metabolomic analysis revealed that BL21 influenced crucial pathways, notably upregulating mTOR signaling and isoflavone biosynthesis, while downregulating the TCA cycle and pyruvate metabolism, suggesting a broad biochemical impact. The probiotic treatment notably improved testicular weight ( p < 0.01) and sperm count and vitality ( p < 0.001) compared to the cyclophosphamide-induced model control, underscoring its efficacy in enhancing reproductive parameters. Additionally, BL21 intervention led to a marked increase in chasing behavior and sex hormone levels, surpassing those of the positive control. The treatment also significantly reduced sperm DNA fragmentation ( p < 0.001) and increased the expression of genes crucial for spermatogenesis and testicular function ( p < 0.05), confirming its potential to restore reproductive health at multiple biological levels. These results highlight the promise of BL21 as a multi-faceted agent for improving reproductive health, warranting further investigation in clinical settings. Probiotic BL21 enhances reproductive parameters in zebrafish by modulating hormone levels, improving sperm quality, and positively affecting reproductive behavior. These findings suggest potential therapeutic applications of probiotics in managing reproductive health issues.
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BMC Oral Health
·
2025
·Vol. 25
·期 1
·1-11
·
IF 3.1
Wang Yuxi,Tang Mengzhen,Yang Xinyu,Yang Jian
Background This study evaluated the penetration, interfacial adaptation, and push-out bond strength of four bioceramic-based root canal sealers (iRoot SP, Well-Root ST, C-Root SP, and KP-Root SP). Methods A total of ninety mandibular first premolar teeth were used in this study, with eighty teeth randomly divided into eight groups ( n = 10). Four groups were designated for sealer penetration analysis, using each of the four sealers mentioned above mixed with 0.1% rhodamine B and applied using the single-cone technique. Horizontal root sections were prepared at 2 mm (apical), 5 mm (middle), and 8 mm (coronal) from the root apex, resulting in a total of 120 slices. Penetration was evaluated using confocal laser scanning microscopy. The other four groups were used for marginal adaptation analysis, with the same sealers applied without rhodamine B, and adaptation was assessed using scanning electron microscopy on sections prepared at the same depths. The remaining ten teeth were used to evaluate push-out bond strength, with 30 dental slices prepared from the middle third, each drilled with four 1 mm diameter holes and randomly filled with one of the four sealers; bond strength was measured using a universal testing machine. Results There was no statistically significant difference in the depth and circumference of dentin tubule penetration between different materials ( P > 0.05). However, the coronal third was significantly higher than the apical third ( P < 0.001). For iRoot SP, the percentage of dentin tubule penetration circumference at the middle third was significantly higher than that at the apical third ( P < 0.05). Additionally, Well-Root ST demonstrated superior adaptability for interfacial adaptation than C-Root SP at all the sites ( P < 0.05). However, the adaptability of iRoot SP was superior to C-Root SP at the coronal and middle thirds ( P < 0.05). Moreover, the push-out bond strength conformed to the following order: Well-Root ST > iRoot SP > KP-Root SP > C-Root SP, with notable variations ( P < 0.05). Conclusion The Well-Root ST sealer demonstrated the best interface adaptation and push-out bonding strength, as well as iRoot SP showed better permeability.
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