ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2015
·Vol. 408
·期 4
·1231-1236
·
IF 3.436
He Yi,Wang Zeru,Long Dengying
The increasing application of nanomaterials will inevitably lead to their release into the environment, which may pose a threat to the environment and human health. As such, there is an urgent need to detect various nanomaterials. In the present work, we present a novel, rapid, and simple visual detection of MnO 2 nanosheets in buffer solution and environmental water sample. In this assay, a redox reaction between MnO 2 nanosheets and 3,3′,5,5′-tetramethylbenzidine (TMB) occurred, leading to the oxidation of TMB to TMB diimine. This redox reaction generated different colors dependent on the concentration of MnO 2 nanosheets, including colorless, blue, green, and yellow, which allowed semiquantitative detection of MnO 2 nanosheets with the naked eye. The detection range of the visual assay was 2–15 μg/mL, and the minimum concentration of MnO 2 nanosheets can be visually detected at concentrations down to 2.0 μg/mL. Moreover, the developed visual assay showed a high selectivity to MnO 2 nanosheets over Mn 2+ ions, tetramethylammonium hydroxide, hydrogen peroxide, graphene oxide, and graphitic carbon nitride nanosheets.
Visual detection of MnO 2 nanosheets
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
·
2015
·Vol. 27
·期 1
·1-8
·
IF 2.587
Dong Hua,Tang Guannan,Ma Ting,Cao Xiaodong
In this paper, we report one-step fabrication of poly(lactide-co-glycolic acid)/titanium oxide (PLGA/TiO 2 ) hybrid microspheres with tunable surface texture via droplet-based microfluidics. Surface texture of microspheres can be continuously tuned by changing the mass ratio between titanium tetraisopropoxide (TTIP) and PLGA in the dispersed phase. The fast hydrolysis of TTIP on the droplet surface can generate a thin shell membrane, resulting in a wrinkled surface after extraction of organic solvent. In vitro drug release monitoring of tanshinone IIA-loaded PLGA/TiO 2 hybrid microsphere reveals that surface texture can affect the drug release rate to a large extent without sacrificing the drug encapsulation efficiency. Our finding might benefit the sustained drug delivery where variable drug release rate and high drug encapsulation efficiency are both required.
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MICROCHIMICA ACTA
·
2014
·Vol. 182
·期 5
·1071-1077
·
IF 3.719
Dong Yajuan,Su Ming,Chen Peiyun,Sun Hanwen
Aqueous solutions of carbon dots (C-dots) were prepared by microwave-assisted thermal carbonization of poly(ethylene glycol). They were investigated by transmission electron microscopy, absorption and fluorescence spectra. It is shown that diperiodato-nicklate(IV), a strong oxidant, induces the chemiluminescence (CL) of C-dots in strongly alkaline solution without use of an additional reagent. A mechanism for this reaction is suggested. It is also found that the CL of the system is quenched by paracetamol, L-cysteine and glutathione. Under the optimized conditions, the calibration plot is linear with a correlation coefficient (r) of >0.995. The limits of detection are 90, 8, and 60 µg L- 1 for paracetamol, L-cysteine, and glutathione, respectively. Spiked urine and serum samples were analyzed and gave recoveries in the range from 84.38 to 116.0 %, with an RSD of 1.2–2.7 %.
Use of diperiodato-nickelate(IV) induces the chemiluminescence of carbon dots in alkaline solution without use of an additional reagent. And using inhibitory effect of the target analyte on the CL achieved the determination of paracetamol, L-cysteine and glutathione.
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Scientific Reports
·
2017
·Vol. 7
·期 1
·1-10
·
IF 4.259
Zhang Zhan,Wang Di,Qiao Shanlei,Wu Xinyue,Cao Shuyuan,Wang Li,Su Xiaojian,Li Lei
Hepatocellular carcinoma (HCC) treatment remains lack of effective chemopreventive agents, therefore it is very attractive and urgent to discover novel anti-HCC drugs. In the present study, the effects of chlorogenic acid (ChA) and caffeic acid (CaA) on HCC induced by diethylnitrosamine (DEN) were evaluated. ChA or CaA could reduce the histopathological changes and liver injury markers, such as alanine transarninase, aspartate aminotransferase, alkaline phosphatase, total bile acid, total cholesterol, high density lipoprotein cholesterol and low density lipoprotein cholesterol. The underlying mechanisms were investigated by a data integration strategy based on correlation analyses of metabonomics data and 16 S rRNA gene sequencing data. ChA or CaA could inhibit the increase of Rumincoccaceae UCG-004 and reduction of Lachnospiraceae incertae sedis , and Prevotella 9 in HCC rats. The principal component analysis and partial least squares discriminant analysis were applied to reveal the metabolic differences among these groups. 28 different metabolites showed a trend to return to normal in both CaA and ChA treatment. Among them, Bilirubin, L-Tyrosine, L-Methionine and Ethanolamine were correlated increased Rumincoccaceae UCG-004 and decreased of Lachnospiraceae incertae sedis and Prevotella 9 . These correlations could be identified as metabolic and microbial signatures of HCC onset and potential therapeutic targets.
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JOURNAL OF NANOPARTICLE RESEARCH
·
2017
·Vol. 19
·期 5
·1-15
·
IF 2.02
Cheng Yang,Niu Helin,Chen Jingshuai,Song Jiming,Mao Changjie,Zhang Shengyi,Chen Changle,Gao Yuanhao
The hierarchical flower-like β-In 2 S 3 catalyst assembled from 2D nanosheets was prepared using an organic-component depletion method utilizing inorganic-organic hybrids indium diethyldithiocarbamate (In-DDTC) as a single-source precursor. The crystallization, morphology and composition of the as-synthesized β-In 2 S 3 were characterized by XRD, SEM, TEM, EDS and XPS, respectively. The β-In 2 S 3 possessed high specific surface area of 134.1 m 2 g -1 , adsorption capacity of 195.5 mg g -1 for methylene blue, and extreme photodecolorization speed under visible light irradiation for the complete removal of methyl orange (MO) dye within 15 min and tetracycline within 60 min. Although methyl orange concentration decreased quickly, the total organic carbon (TOC) decreased slowly. UV-vis and mass spectrometry (MS) were applied to analyze the intermediates coming from the photodecolorization of MO. In order to estimate the roles of active species during the decolorization of MO, trapping experiments were conducted to determine the main active species during the decolorization process. The results indicated that . O 2
− radicals and e -1 were the key intermediates. This enhanced activity was attributed to its unique structures assembled from 2D nanosheets with thickness of ca. 5-7 nm, leading to high specific surface area, wide range of pore size distribution and great efficiency in absorbing light and electron/hole separation. The hierarchical flower-like β-In 2 S 3 demonstrated great advantages in the treatment of various wastewater pollutants including textile dyes and antibiotics.
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JOURNAL OF MATERIALS SCIENCE
·
2017
·Vol. 52
·期 12
·7428-7438
·
IF 2.599
Chen Yipeng,Wang Hanwei,Yao Qiufang,Fan Bitao,Wang Chao,Xiong Ye,Jin Chunde,Sun Qingfeng
The surfaces of plants represent multifunctional interfaces between the organisms and the environment. In this paper, biomimetic taro leaf-like structures with superparamagnetic and superhydrophobic performances were exactly copied on the wood surface through the soft lithography to improve the wood properties. Fe 3 O 4 nanoparticles were mixed into poly(dimethylsiloxane) PDMS suspensions to obtain Fe 3 O 4 /PDMS suspensions that commonly endow coats magnetic and microwave absorption properties, which were then cast onto the wood surface and packaged by PDMS stamps replicated from fresh taro leaves. Fe 3 O 4 /PDMS films, which coexisted superhydrophobic surface and superparamagnetic property, were created on the wood surface after the being dried and stamps were peeled off. The as-prepared wood surface exhibited unique taro leaf-like micro- and nanostructures, microwave absorption, superparamagnetism performances with maximum saturation magnetization ( M
s ) of 22.9 emu g −1 and superior static superhydrophobicity with a water contact angle of 152° ± 2°. This research may provide a feasible pathway for constructing naturally biomorphic structures on the wood surface with tailored functions.
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Nature Photonics
·
2024
·Vol. 18
·期 2
·162-169
·
IF 32.3
Du Xinyuan,Zhao Shan,Wang Lu,Wu Haodi,Ye Fan,Xue Kan-Hao,Peng Shaoqian,Xia Jianlong,Sang Ziru,Zhang Dongdong,Xiong Zuping,Zheng Zhiping,Xu Ling,Niu Guangda,Tang Jiang
Efficient and fast scintillators are in high demand in a variety of fields, such as medical diagnostics, scientific instruments and high-energy physics. However, the trade-off between high scintillation efficiency and fast timing properties is a common challenge facing almost all scintillators. To overcome this limitation, we have developed a strategy for organic scintillators by directing all hot excitons into fast singlet emission states without involving the lowest triplet states. Our scintillator, 1,1,2,2-tetrakis(4-bromophenyl)ethylene, shows an ultrafast radiative lifetime of 1.79 ns and a light yield of ∼ 34,600 photons per MeV, exhibiting an excellent combination of high light yield and short decay time. Our work provides a method to design efficient and ultrafast scintillators, and paves the way towards exciting applications for ultrafast detection and imaging.
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Journal of Neuroimmune Pharmacology
·
2026
·Vol. 21
·期 1
·53
·
IF 6.1
Zhao Jun,Bai Yiming,Wang Chao,Guo Pengfei,Wang Zhe,Du Guanhua,Liu Ailin
Therapeutic for Alzheimer’s disease (AD) remains a pressing medical challenge in the field of neuroscience. Natural compounds with structural diversity and multi-target regulatory properties have attracted increasing attention for the treatment of complex neurodegenerative disorders. Fraxetin, previously identified through computer-aided virtual screening, C. elegans assays, and in vitro cellular models as a potential anti-AD natural compound, was further investigated in this study using established in vivo and in vitro systems to evaluate its efficacy in ameliorating cognitive function and elucidate underlying mechanisms. A sporadic AD mouse model was established by intracerebroventricular injection of STZ (3 mg/kg). Fraxetin (3, 10, and 30 mg/kg/day, po.) was administered until the mouse sacrifices. Cognitive performance was evaluated using the novel object recognition, passive avoidance, and Morris water maze tests. STZ induced cerebral energy metabolism disturbances accompanied by mitochondrial dysfunction, neuroinflammation, oxidative stress, neuronal and synaptic loss, and cholinergic system impairment. Behavioral tests confirmed that fraxetin ameliorated cognitive deficits in STZ-induced mice. Further studies revealed that fraxetin preserved mitochondrial structural integrity and enhanced mitochondrial biogenesis, accompanied by upregulation of the Sirt1/PGC-1α/Nrf1/TFAM pathway. In parallel, fraxetin attenuated microglial overactivation and neuroinflammatory responses. Consistently, in LPS-stimulated BV2 microglial cells, fraxetin inhibited nitric oxide and inflammatory mediator release, which was associated with modulation of the TLR2/MYD88/NF-κB signaling pathway, while restoring cellular bioenergetic status, indicating coordinated regulation of inflammation-associated metabolic dysfunction. Importantly, fraxetin attenuated neurodegenerative alterations in STZ-treated mice, as evidenced by reduced neuronal loss and synaptic degeneration. These effects were associated with activation of the BDNF/TrkB/ERK/CREB neurotrophic pathway and inhibition of acetylcholinesterase activity, supporting preservation of synaptic and cholinergic function. This study provides experimental evidence supporting fraxetin as a promising natural multi-target candidate for the intervention of AD.
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INFLAMMATION
·
2026
·
IF 5.4
Wang Zhengwei,Lin Jiani,Tan Guisen,Zhang Weimin,Chen Tao,Gao Ming,Liu Jiacheng,Wang Jianwei,Pan Tingzheng,Li Qingzhong,Zhang Hongtian,Shen Hongwei,He Xiaosheng,Liu Jiangang,Liu Zhuohao
The post-ischemic immune microenvironment critically balances neuroinflammation and tissue repair, making macrophage polarization a pivotal therapeutic target in cerebral infarction. Umbilical cord mesenchymal stromal cells (UCMSCs) modulate macrophage polarization primarily through paracrine signaling, while the key mediators and underlying mechanisms remain incompletely elucidated. Using proteomic array-based screening of co-culture supernatants, we identified soluble factors secreted by UCMSCs that regulate macrophage polarization. Stable soluble tumor necrosis factor receptor 1 (sTNFR1)-overexpressing (oe-sTNFR1) UCMSCs were generated via lentiviral transduction. The mechanisms by which sTNFR1 regulates macrophage polarization were investigated in vitro using immunoblotting, RT-qPCR, immunofluorescence, and flow cytometry. The therapeutic efficacy was evaluated in a rat model of middle cerebral artery occlusion (MCAO) via intracranial delivery of oe-sTNFR1 UCMSCs, with outcomes assessed via histological (Nissl and 2,3,5-triphenyltetrazolium chloride) staining, behavioral tests, and magnetic resonance imaging. To circumvent risks of genetic modification, a TNF-α-targeting nanobody (TNF-α Nb) was developed and tested in combination with wild-type UCMSCs. Proteomic analysis identified sTNFR1 as a key mediator secreted by UCMSCs for reprogramming macrophage polarization. oe-sTNFR1 UCMSCs enhanced macrophage chemotaxis and promoted reparative polarization in a paracrine-dependent manner. Mechanistically, UCMSC-derived sTNFR1 suppressed the NF-κB axis, which not only attenuated pro-inflammatory polarization but also derepressed SOCS3 signaling, thereby promoting the reparative phenotype. In MCAO rats, intracranial delivery of oe-sTNFR1 UCMSCs reshaped the post-ischemic brain microenvironment toward an anti-inflammatory and pro-repair state, improving neurological recovery through reduced infarct volume and improved functional outcomes. Furthermore, co-administration of the TNF-α Nb with wild-type UCMSCs enhanced a reparative myeloid phenotype and improved neurological outcomes compared to UCMSCs alone. This study establishes UCMSC-derived sTNFR1 as a key paracrine mediator of macrophage polarization via the sTNFR1/NF-κB/SOCS3 axis, facilitating functional recovery after ischemic stroke. The strategy combining a TNF-α Nb with UCMSCs offers a novel and clinically promising immunotherapeutic approach.
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MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 11
·729
·
IF 5.4
Wang Mengjun,Huang Rong,Yang Xinwen,Zeng Minghao,Wang Huizhen,Luo Xiaojun
A ratiometric fluorescence probe was developed by integrating a covalent organic framework (TP-BDB-COF) with N-doped quantum dots (N-CDs) encapsulated in zeolitic imidazolate framework-8 (N-CDs@ZIF-8), enabling highly sensitive and selective detection of hypochlorite (ClO⁻) in aqueous environments. The yellow-orange-emissive TP-BDB-COF, synthesized by the condensation of 2,4,6-triformylphloroglucinol (TP) and 3,3’-dimethyl-[1,1’-biphenyl]-4,4’-diamine (BDB), exhibited specific fluorescence quenching at 570 nm upon interaction with ClO⁻. The blue-emissive N-CDs@ZIF-8 served as a stable reference signal at 430 nm, enabling self-calibration via the ratiometric signal (F 430 /F 570 ). This dual-signal strategy significantly improved detection accuracy and reliability. This sensor exhibited a broad linear fluorometric response range of 0.05 to 200 µM for ClO⁻, with an exceptionally low detection limit of 22 nM. Moreover, its practical applicability was validated through analysis of real water samples, yielding satisfactory spike recoveries between 98.53% and 101.30%. This approach not only offers a robust method for monitoring ClO⁻ in complex matrices but also provides a versatile framework for constructing high-performance ratiometric sensors. Graphical abstract
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Journal of Neuroimmune Pharmacology
·
2024
·Vol. 19
·期 1
·1-17
·
IF 5.2
Lin Yun,Liu Tian,Chen Hong,Zeng Ming,Hu Shunwei,Yu Xiaoning,Chen Yonghua,Xia Chunmei,Wang Jin,Wang Jijiang
The occurrence of major asthma symptoms is largely attributed to airway vagal hypertonia, of which the central mechanisms remain unclear. This study tests the hypotheses that endothelin-1-mediated brainstem glial activation produces asthmatic airway vagal hypertonia via enhanced action of adenosine 5’-triphosphate on neuronal purinergic P2X4 receptors. A rat model of asthma was prepared using ovalbumin. Airway vagal tone was evaluated by the recurrent laryngeal discharge and plethysmographic measurement of pulmonary function. The changes in the brainstem were examined using ELISA, Western blot, luciferin-luciferase, quantitative reverse transcription-polymerase chain reaction, enzyme activity assay and immunofluorescent staining, respectively. The results showed that in the medulla of rats, endothelin receptor type B and P2X4 receptors were primarily expressed in astrocytes and neurons, respectively, and both of which, along with endothelin-1 content, were significantly increased after ovalbumin sensitization. Ovalbumin sensitization significantly increased recurrent laryngeal discharge, which was blocked by acute intracisternal injection of P2X4 receptor antagonist 5-BDBD, knockdown of brainstem P2X4 receptors, and chronic intraperitoneal injection of endothelin receptor type B antagonist BQ788, respectively. Ovalbumin sensitization activated microglia and astrocytes and significantly decreased ecto-5’-nucleotidase activity in the medulla, and all of which, together with the increase of medullary P2X4 receptor expression and decrease of pulmonary function, were reversed by chronic BQ788 treatment. These results demonstrated that in rats, allergic airway challenge activates both microglia and astrocytes in the medulla via enhanced endothelin-1/endothelin receptor type B signaling, which subsequently causes airway vagal hypertonia via augmented adenosine 5’-triphosphate/P2X4 receptor signaling in central neurons of airway vagal reflex. Graphical Abstract
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Journal of Neuroinflammation
·
2024
·Vol. 21
·期 1
·1-17
·
IF 9.3
Gao Dandan,Zou Bin,Zhu Kunyuan,Bi Shijun,Zhang Wenxu,Yang Xinyu,Lai Jieyu,Liang Guobiao,Pan Pengyu
Background Subarachnoid hemorrhage (SAH) is a severe cerebrovascular disorder primarily caused by the rupture of aneurysm, which results in a high mortality rate and consequently imposes a significant burden on society. The occurrence of SAH initiates an immune response that further exacerbates brain damage. The acute inflammatory reaction subsequent to SAH plays a crucial role in determining the prognosis. Th17 cells, a subset of T cells, are related to the brain injury following SAH, and it is unclear how Th17 cells are cleared in the brain. Meningeal lymphatic vessels are a newly discovered intracranial fluid transport system that has been shown to drain large molecules and immune cells to deep cervical lymph nodes. There is limited understanding of the role of the meningeal lymphatic system in SAH. The objective of this research is to explore the impact and underlying mechanism of drainage Th17 cells by meningeal lymphatics on SAH.Methods Treatments to manipulate meningeal lymphatic function and the CCR7-CCL21 pathway were administered, including laser ablation, injection of VEGF-C geneknockout, and protein injection. Mouse behavior was assessed using the balance beam experiment and the modified Garcia scoring system. Flow cytometry, enzyme-linked immunosorbent assays (ELISA), and immunofluorescence staining were used to study the impact of meningeal lymphatic on SAH drainage. Select patients with unruptured and ruptured aneurysms in our hospital as the control group and the SAH group, with 7 cases in each group. Peripheral blood and cerebrospinal fluid (CSF) samples were assessed by ELISA and flow cytometry.Results Mice with SAH showed substantial behavioral abnormalities and brain damage in which immune cells accumulated in the brain. Laser ablation of the meningeal lymphatic system or knockout of the CCR7 gene leads to Th17 cell aggregation in the meninges, resulting in a decreased neurological function score and increased levels of inflammatory factors. Injection of VEGF-C or CCL21 protein promotes Th17 cell drainage to lymph nodes, an increased neurological function score, and decreased levels of inflammatory factors. Clinical blood and CSF results showed that inflammatory factors in SAH group were significantly increased. The number of Th17 cells in the SAH group was significantly higher than the control group. Clinical results confirmed Th17 cells aggravated the level of neuroinflammation after SAH.Conclusion This study shows that improving the drainage of Th17 cells by meningeal lymphatics via the CCR7-CCL21 pathway can reduce brain damage and improve behavior in the SAH mouse model. This could lead to new treatment options for SAH.
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