CEREAL CHEMISTRY
·
2017
·Vol. 94
·期 3
·464-470
·
IF 0.978
Na-Na Wu,Bin Tan,Sha-Sha Li,Xiao-Hong Tian,Ming Liu,Yan-Xiang Liu,Li-Ping Wang,Xiao-Tong Zhai
The effect of extruded brown rice flour (EBR) contents (0–50%) on antioxidant activity, phenolics, in vitro digestibility, color, and cooking quality of noodles containing mixtures of wheat and EBR was investigated. The antioxidant activity and phenolic content increased, especially ferulic and coumaric acids in bound forms, whereas the in vitro glycemic index, optimal cooking time, water absorption, hardness, and color were diminished in noodles with the addition of EBR; cooking loss increased as a function of the EBR percentage. The partial replacement of wheat flour with EBR can be favorably used in the wheat noodle formulation. The results provide the basis for the development of staple foods with nutritional characteristics for today's functional food markets.
研究领域:
食品科学
·
营养学
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产品关联
DOI
高影响因子
MOLECULAR PLANT-MICROBE INTERACTIONS
·
2020
·
IF 3.696
Huanpeng Li,Jiaojiao Wu,Xiaofeng Shang,Miaomiao Geng,Jing Gao,Shuqing Zhao,Xiumei Yu,Daqun Liu,Zhensheng Kang,Xiaojie Wang,Xiaodong Wang
In Arabidopsis , both pathogen invasion and benzothiadiazole (BTH) treatment activate the nonexpresser of pathogenesis-related genes 1 ( NPR1 )-mediated systemic acquired resistance, which provides broad-spectrum disease resistance to secondary pathogen infection. However, the BTH-induced resistance in Triticeae crops of wheat and barley seems to be accomplished through an NPR1 -independent pathway. In the current investigation, we applied transcriptome analysis on barley transgenic lines overexpressing wheat wNPR1 ( wNPR1-OE ) and knocking down barley HvNPR1 ( HvNPR1-Kd ) to reveal the role of NPR1 during the BTH-induced resistance. Most of the previously designated barley chemical-induced ( BCI ) genes were upregulated in an NPR1 -independent manner, whereas the expression levels of several pathogenesis-related ( PR ) genes were elevated upon BTH treatment only in wNPR1-OE. Two barley WRKY transcription factors, HvWRKY6 and HvWRKY70 , were predicted and further validated as key regulators shared by the BTH-induced resistance and the NPR1 -mediated acquired resistance. Wheat transgenic lines overexpressing HvWRKY6 and HvWRKY70 showed different degrees of enhanced resistance to Puccinia striiformis f. sp. tritici pathotype CYR32 and Blumeria graminis f. sp. tritici pathotype E20. In conclusion, the transcriptional changes of BTH-induced resistance in barley were initially profiled, and the identified key regulators would be valuable resources for the genetic improvement of broad-spectrum disease resistance in wheat.
研究领域:
植物病理学
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分子生物学
·
遗传学
·
农学
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产品关联
DOI
PubMed
高影响因子
MOLECULAR PLANT-MICROBE INTERACTIONS
·
2018
·Vol. 32
·期 2
·
IF 3.588
Dandan Zhang,Caijuan Tian,Kangquan Yin,Wenyi Wang,Jin-Long Qiu
Stomata are leaf pores that regulate gas exchange and water transpiration in response to environmental cues. They also function in innate immunity by limiting pathogen entry through actively closing in so-called stomatal defense. However, roles of stomata in plant disease resistance are not fully elucidated, especially in monocots. Here, we report that non–race specific resistance of the rice abscisic acid-deficient mutant Osaba1 to Xanthomonas oryzae pv. oryzae is due to increased stomatal conductance. Reducing stomatal conductance in the Osaba1 mutant increases its susceptibility to X. oryzae pv. oryzae . Artificial opening of stomata in wild-type plants leads to enhanced resistance to X. oryzae pv. oryzae . The rice mutant es1-1 with constitutively higher stomatal conductance exhibits strong resistance to X. oryzae pv. oryzae . Additionally, Osaba1 and es1-1 are resistant to X. oryzae pv. oryzicola . The data support that open stomata confer postinvasive resistance against bacterial pathogens in rice, and such resistance probably results from decreased leaf water potential. Our findings reveal a novel role of stomata in plant immunity through modulation of leaf water status, which provides physiological insight into the interactions between plant, pathogen, and environment.
研究领域:
植物学
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分子生物学
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植物病理学
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产品关联
DOI
PubMed
高影响因子
PHYTOPATHOLOGY
·
2020
·Vol. 110
·期 5
·
IF 3.234
Ping Yang,Fang-Jing Li,Shi-Wen Huang,Man Luo,Wei Lin,Gao-Qing Yuan,Qi-Qin Li
Berberine, a botanical drug, has great ability to inhibit the growth of Xanthomonas oryzae pv. oryzae . However, the antibacterial mechanism of berberine against X. oryzae pv. oryzae remains poorly understood. In this study, we investigated the physiological and transcriptional response of X. oryzae pv. oryzae to berberine. When strain X. oryzae pv. oryzae GX13 was treated with berberine (10 µg/ml), the hypersensitive response in tobacco, virulence to rice, pathogen population in the rice xylem, production of extracellular polysaccharide (EPS), and activity of extracellular hydrolases decreased, but the levels of pyruvate and ATP increased. Moreover, biofilm formation was inhibited, and the cell membrane was damaged. Transcriptome sequencing analysis showed downregulated expression of gspD , gspE , and gspF , involved in the type II secretion system (T2SS); hrcC , hrcJ , hrcN , and others, involved in the type III secretion system (T3SS); gumB and gumC , associated with EPS; zapE , ftsQ , and zapA , associated with cell division; lpxH , lpxK , kdtA , and others, associated with the membrane; and pyk , pgk , and mdh , encoding pyruvate kinase, phosphoglycerate kinase, and malate dehydrogenase, respectively. Upregulated expression was observed for nuoA , nuoB , and nuoH , encoding the NADH dehydrogenase complex, and atpF , atpC , and atpB , encoding ATP synthase. An adenylate cyclase ( CyaA ) fusion assay showed that berberine affects type three effector protein secretion via the T3SS and reduces effector translocation in X. oryzae pv. oryzae . It is speculated that the negative growth and virulence phenotypes of berberine-treated X. oryzae pv. oryzae GX13 may involve differentially expressed genes associated with cytoarchitecture and energy metabolism, and these effects on primary cell function may further dampen virulence and result in differential expression of T3SS- and T2SS-related genes.
研究领域:
植物病理学
·
微生物学
·
分子生物学
·
药理学
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产品关联
DOI
PubMed
高影响因子
MOLECULAR PLANT-MICROBE INTERACTIONS
·
2021
·
IF 4.171
Xingtao Bao,Rui Yang,Shilong Jiang,Jinping Zhao,Delu Wang,Dongxue Li,Xian Wu,Baoan Song,Zhuo Chen
Diseases caused by fungi can affect the quality and yield of the leaves of tea [ Camellia sinensis (L.) Kuntze]. At present, the availability of highly effective and safe fungicides for controlling tea plants remains limited. The objectives of this study were to identify novel compounds with antifungal activities and to determine their molecular mechanisms. A series of sulfone compounds containing 1,3,4-oxadiazole were evaluated in China for their antifungal activities against several pathogens causing foliar diseases and high production losses. Transcriptomics and bioinformatics were used to analyze the differentially expressed genes of Lasiodiplodia theobromae treated with a representative compound, jiahuangxianjunzuo (JHXJZ). Moreover, the effects of JHXJZ on ergosterol content, membrane permeability, cell structure, and seven key genes involved in the ergosterol biosynthetic pathway were investigated. JHXJZ had a strong antifungal activity against L. theobromae in vitro, with an effective concentration giving 50% inhibition of 3.54 ± 0.55 μg/ml, and its curative efficacies on detached tea leaves reached 41.78% at 100 μg/ml. JHXJZ upregulated 899 genes ( P < 0.05) and downregulated 1,185 genes ( P < 0.05) in L. theobromae . These genes were found to be associated with carbohydrate metabolic processes, which are closely related to steroid biosynthesis in the Kyoto Encyclopedia of Genes and Genomes pathways. Because JHXJZ regulates the key genes of sterol biosynthesis, it decreased the ergosterol content, increased cell-membrane permeability, changed the cellular structure, enhanced the roughness of the surface of the hyphae, and resulted in degradation of the hyphal nuclei and necrosis of the hyphal cytoplasm. Our study demonstrates that JHXJZ is a fungicide with a novel mechanism of action that differs from that of triazole fungicides. JHXJZ has potential for applications in controlling tea plant diseases.
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产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2018
·
IF 4.439
Wenfei Zhou,Mo Yang,Zhi Zhao,Shaopeng Li,Zhiqiang Cheng,Jinsong Zhu
Surface-grafted polymer brushes have been long reported as a desired matrix for biosensing applications, owing to enhanced immobilization capacity and anti-fouling properties. However, simultaneous optimizing binding signal and minimizing non-specific adsorption using polymer matrix is still a challenge, due to the lack of systematic structural and composition optimization at molecular level. In this research, a series of block copolymer brushes with controlled hierarchical architectures, poly [oligo (ethylene glycol) methacrylate- b -glycidyl methacrylate] (POEMGA- b -PGMA) were prepared by two-stage surface-initiated polymerization. Surface plasmon resonance imaging (SPRi), as a real-time and label-free biosensing technology, was used as means to evaluate their detecting performances of bio-interactions. In contrast to one-layer poly (glycidyl methacrylate) (PGMA), 4.7-fold enhancement of signal-to-background ( S / B ) was achieved in bi-layer POEGMA- b -PGMA surface, by using rabbit IgG (R-IgG, in 10% serum) and a microarray of goat-anti-rabbit IgG (G-R-IgG) as the system of study. Interestingly, the binding signals and anti-fouling performances of the polymer brushes could be enhanced simultaneously by increasing the length of the monomer in the primary layer. Theoretically, it mainly owed to the formation of a densely packing protective primary layer against non-specific physical adsorption and a spatially optimized secondary layer of minimized steric hindrance that allowed optimum binding of analytes.
研究领域:
材料科学
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生物传感器
·
高分子化学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
SPECTROSCOPY LETTERS
·
2025
·
IF 1.6
Ju Wei,Bingyu Luo,Jiao Fu,Shunli Zhou,E. Li,Xiaodong Su,Xiaotong Gao,Zhenfu Jia,Xiaojiang Li
To overcome the interference caused by the inner filter effect, this study optimally selected red fluorescence carbon dots as the signal source and constructed a novel dual-mode Ag@Red-CDs nanoprobe for the highly selective detection of Fe³+ using fluorescence and colorimetry. This probe is based on a unique reaction between Fe3+ and silver nanoparticles (AgNPs) that weakens the static quenching effect of AgNPs on R-CDs; at the same time, the depletion of AgNPs induces a change in the absorbance of the system. Under optimal conditions, the probe exhibited a linear fluorescence response in the Fe³+ concentration range of 0.10–25.00 μg/mL and a linear colorimetric response of 0.18–8.00 μg/mL. The detection limits for fluorescence and colorimetry were 0.31 μg/mL and 0.56 μg/mL, respectively. In spiked real samples, the fluorescence method achieved Fe³+ recovery rates of 99.10–102.34%, while the colorimetric method showed recovery rates of 99.83–101.42%. Overall, this research significantly broadened the potential applications of R-CDs-based materials for the quantitative detection of heavy metal ions in water bodies.
研究领域:
分析化学
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环境科学
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材料科学
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产品关联
DOI
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2024
·
IF 4.8
Danilo de Souza Costa,Rayssa A. Cajas,Carla Monteiro Leal,Lara S. Aleixo de Carvalho,Lara Cruz de Souza,Lucas Fukui-Silva,Josué de Moraes,Ademar A. Da Silva Filho
Ethnopharmacological relevance Acmella oleracea (L.) R. K. Jansen, commonly referred to as “Jambu”, is a valuable medicinal plant native to the Amazon regions. Inflorescences of A. oleracea is utilized as local anesthetic properties and for its insecticidal, antiparasitic, and anthelmintic attributes. Aims of this study This study aimed to evaluate the in vitro and in vivo antiparasitic properties of A. oleracea extract and spilanthol (SPL) against Schistosoma mansoni . Materials and methods The ethanolic extract of A. oleracea inflorescences (AoE) was prepared, analyzed by HPLC-DAD, and characterized by UHPLC-HRMS/MS. SPL was isolated from AoE by chromatographic fractionation. The antischistosomal properties of AoE and SPL were evaluated in vitro against adult schistosomes and in preclinical assays using murine models of patent S. mansoni infection. Results Through UHPLC-HRMS/MS analysis, 14 alkamides were annotated in AoE. HPLC-DAD analysis of AoE revealed a peak with a substantial relative area of ∼85%, which was isolated and identified as SPL. AoE and SPL caused mortality of adult schistosomes ex vivo , showing EC 50 values of 32.6 μg/mL and 27.8 μM, respectively, without affected Vero cells or Caenorhabditis elegans . In preclinical studies, the oral administration (400 mg/kg) of AoE and SPL resulted in significant reductions in worm burden of 28% and ∼42%, respectively. Additionally, SPL exhibits remarkable effects on oogram, decreasing egg burden and the number of immature eggs by over 80%, while also promoting significant reductions in hepato- and splenomegaly. Conclusions The research underscores the antischistosomal activity of A. oleracea highlighting the potential of SPL as a lead for the development of new schistosomicidal drugs and encouraging further studies to validate the traditional anthelmintic use of A. oleracea inflorescences.
研究领域:
药理学
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寄生虫学
·
药物开发
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Huajian Xu,Zhongtao Hu,Zongwei Hao,Changyue Deng,Xiaowen Pi,Binjia Zhang
The effects of amino acids with different R-groups, namely glutamic acid (GLU), glutamine (GLN), and theanine (THE), on the pasting and structure properties of corn starch (CS) were investigated. During gelatinization, GLU decreased viscosity of CS. GLN and THE increased pasting enthalpy of CS from 7.62 to 8.72 and 9.50 J/g, respectively. All starch-amino acid systems had higher storage and loss modulus. Amino acids could adhere to starch granule surface. After co-gelatinization, GLU increased short-range order degree of starch, while GLN and THE decreased. Both infrared spectroscopy and quantum computation showed that non-covalent interaction occurred between amino acids and starch. Molecular dynamics revealed that the interaction between GLU and starch weakened hydrogen bonding between starch and water, promoting cross-linking between starch chains. GLN and THE changed dihedral angle torsion of glycoside bonds, and mainly interacted with starch by electrostatic interaction (50.06 kJ/mol) and van der Waals force (50.30 kJ/mol), respectively.
研究领域:
食品科学
·
化学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF POLYMER RESEARCH
·
2017
·Vol. 24
·期 5
·1-9
·
IF 1.615
Xue Sheng,Yang Haicun,Ma Wenzhong,Gong Fanghong,Tao Guoliang,Liu Chunlin,Pan Ji
Poly(methyl methacrylate) brushes grafted from attapulgite nanoparticles (ATP@PMMA) were prepared by R-Supported reversible addition-fragmentation chain transfer polymerization (RAFT). ATP was firstly activated, and allowed to react with γ-aminopropyltriethoxysilane (APTES), 4,4′-azobis(4-cyanovaleric acid) (ACVA) and bis(thiobenzoyl) disulfide stepwise to afford 4-cyanopentanoic acid dithiobenzoate functionalized ATP (ATP-CPADB). Then, RAFT polymerizations of MMA mediated by the different systems were conducted and compared systemically. The grafting process was verified by FTIR, XRD, XPS, TGA and TEM data. Kinetic behavior indicated that the anchored CPADB is more effective before diffusing into the bulk phase. The grafting ratio ( G
r ), while M
n of grafted and free polymer increased linearly with increasing monomer conversion in presence of free CPADB, giving the hybrid particles with more homogeneous distribution of grafted polymer layer.
研究领域:
材料科学
·
高分子化学
✓ 已验证
DOI
高影响因子
Bioresources and Bioprocessing
·
2019
·Vol. 6
·期 1
·1-12
·
IF 0
Zhang Can,Song Wei,Liu Jia,Chen Xiulai,Liu Liming
( R )- or ( S )-2-Hydroxy-4-(methylthio)butanoic acid (HMTBA) is used as a poultry nutritional supplement and to treat renal failure disease. Herein, we report an artificially designed biocatalytic cascade process, which uses l -methionine to synthesize ( R )- or ( S )-HMTBA. This biocatalysis cascade comprises a basic module and two different extender modules and operates in a modular assembly manner. The basic module responsible for the transformation of l -methionine to α-keto-γ-methylthiobutyric acid (KMTB) is comprised of the l -amino acid deaminase. Two different extender modules responsible for the transformation of KMTB to ( R )- or ( S )-HMTBA are comprised of the R/S-specific lactate dehydrogenase in combination with the formate dehydrogenase, respectively. Engineered Escherichia coli catalysts, one containing the basic module, the other containing the one of two different extender modules, produced 97.6 g L −1 ( R )-HMTBA and 96.4 g L −1 ( S )-HMTBA with a yield of 96.9% and 95.8% at the large scale (1 L) using a two-stage strategy in one pot, respectively. Therefore, this biocatalytic process lays the foundation for the industrial-scale conversion of low-cost l -amino acids to corresponding high-value enantiopure chiral 2-hydroxy acids.
研究领域:
生物技术
·
酶工程
·
代谢工程
·
合成生物学
✓ 已验证
DOI
Journal of Ginseng Research
·
2022
·
IF 5.735
Hong-Yan Guo,Yue Xing,Yu-Qiao Sun,Can Liu,Qian Xu,Fan-Fan Shang,Run-Hui Zhang,Xue-Jun Jin,Fener Chen,Jung Joon Lee,Dongzhou Kang,Qing-Kun Shen,Zhe-Shan Quan
Background Ginseng possesses antitumor effects, and ginsenosides are considered to be one of its main active chemical components. Ginsenosides can further be hydrolyzed to generate secondary saponins, and 20( R )-panaxotriol is an important sapogenin of ginsenosides. We aimed to synthesize a new ginsengenin derivative from 20( R )-panaxotriol and investigate its antitumor activity in vivo and in vitro . Methods Here, 20( R )-panaxotriol was selected as a precursor and was modified into its derivatives. The new products were characterized by 1 H-NMR, 13 C-NMR and HR-MS and evaluated by molecular docking, MTT, luciferase reporter assay, western blotting, immunofluorescent staining, colony formation assay, EdU labeling and immunofluorescence, apoptosis assay, cells migration assay, transwell assay and in vivo antitumor activity assay. Results The derivative with the best antitumor activity was identified as 6,12-dihydroxy-4,4,8,10,14-pentamethyl-17-(2,6,6-trimethyltetrahydro-2 H -pyran-2-yl)hexadecahydro-1 H -cyclopenta[a]phenanthren-3-yl( tert -butoxycarbonyl)glycinate ( A11 ). The focus of this research was on the antitumor activity of the derivatives. The efficacy of the derivative A11 (IC 50 < 0.3 μM) was more than 100 times higher than that of 20( R )- panaxotriol (IC 50 > 30 μM). In addition, A11 inhibited the protein expression and nuclear accumulation of the hypoxia-inducible factor HIF-1α in HeLa cells under hypoxic conditions in a dose-dependent manner. Moreover, A11 dose-dependently inhibited the proliferation, migration, and invasion of HeLa cells, while promoting their apoptosis. Notably, the inhibition by A11 was more significant than that by 20( R )-panaxotriol ( p < 0.01) in vivo . Conclusion To our knowledge, this is the first study to report the production of derivative A11 from 20( R )-panaxotriol and its superior antitumor activity compared to its precursor. Moreover, derivative A11 can be used to further study and develop novel antitumor drugs.
研究领域:
药理学
·
肿瘤学
·
有机化学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）