ANALYTICAL SCIENCES
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2023
·Vol. 39
·期 10
·1719-1726
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IF 1.6
Hong Li-Na,Cao Hui-Ting,Feng Yi-Xuan,Guo Li-Zhen,Liu Meng-Qian,Zhang Kun,Mai Xi,Li Na
Designing ratiometric sensors for cysteine (Cys) monitoring with high accuracy is of great significance for disease diagnosis and biomedical studies. The current ratiometric methods mainly rely on multiplex probes, which not only complicates the operation but also increases the cost, making it difficult for quantitative Cys detection in resource-limited areas. Herein, one-pot prepared gold nanoclusters (Au NCs) that glow red fluorescent were synthesized by employing glutathione as the stabilizer and reducing agent. When Fe 3+ is present with Au NCs, the fluorescence is quenched and the scattering is strong because of the aggregation of Au NCs. With introduction of Cys, Cys can efficiently compete with glutathione-modified Au NCs for Fe 3+ , which leads to increase of fluorescence and decrease of scattering. The ratiometric determination of Cys can be thereby realized by collecting the fluorescence and SRS spectrum simultaneously. The linear range for Cys was 5–30 µM with a detection limit of 1.5 µM. In addition, the sensing system exhibits good selectivity for Cys and shows potential application in biological samples. Graphical Abstract
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MACROMOLECULES
·
2023
·
IF 5.5
Dianyu Dong,Yuzhang Zhu,Wangxi Fang,Jian Jin
Ultrathin membranes by interfacial polymerization can efficiently purify high-salinity water, but the variety beyond classic polyamide is limited yet. Based on the modular and rapid feature of click chemistry, this work for the first time reports a “clickable” IP strategy to prepare polythioether ultrathin membranes for ion separation. Multiple sets of multifunctional thiol and alkene/alkyne monomers are proven to react at the water–oil interface via thiol–ene/yne addition polymerization. In particular, triallyl cyanurate-DL-dithiothreitol and triallyl isocyanurate-DL-dithiothreitol ultrathin membranes prepared on polyether sulfone substrates reach the separation accuracy of nanofiltration with rejections to Na2SO4 of over 95%. Their surfaces remain negatively charged in both acidic and alkaline environments, and thioether linkages even stand the challenge of long-term acid treatment with high concentration. This strategy would thus motivate growing attempts at developing new-type ultrathin membranes for ion removal or desalination.
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International Journal of Electrochemical Science
·
2023
·
IF 1.5
Hong-Wei Lv,Quan-Fu Li,Hui-Ling Peng
Sodium tetrafluorocuprate (Na 2 CuF 4 ) has been synthesized by the precipitation method. Then, Na 2 CuF 4 was applied to glucose electrocatalysis for the first time, and modified bare Au electrode to obtain Na 2 CuF 4 /Au. Na 2 CuF 4 /Au has strong anti-interference, extremely broad linear range of glucose detection, and great stability through stored for a long time at room temperature. A simple method was used to prepare Na 2 CuF 4 with different morphologies, and the effects of different morphologies for the glucose sensing performance were studied. The optimal experimental parameters of glucose sensing were obtained through parameter optimization, which leads to enhanced glucose sensing performance. The selected Na 2 CuF 4 was composed of large crystals and smaller crystals. The larger crystals sizes of selected Na 2 CuF 4 are in the range from 5 μm to 6 μm, and the smaller are in the range from 230 nm to 300 nm, the two are mixed together. The Na 2 CuF 4 /Au electrode showed high sensitivity and very wide linear range (the first 0.01-0.15 mM, the second 0.15-19.15 mM, the third 19.15-63.15 mM). The Na 2 CuF 4 /Au shows good accuracy, reproducibility and stability, as well as excellent selectivity. The properties of Na 2 CuF 4 indicate that it has a wide application prospect in the field of glucose electrocatalysis as a new glucose sensitive material.
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Nano Research
·
2023
·Vol. 16
·期 8
·11042-11047
·
IF 9.9
Wang Lei,Zhang Yuehan,Sun Xiaoxuan,Li Yunhui,Zhai Junfeng,Dong Shaojun
Eco-friendly chemical oxygen demand (COD) sensors are highly desired with respect to the importance of COD determination in environmental protection. In this work, a new FTO/TiO 2 /PbO 2 (FTO = fluorine-doped tin oxide) electrode was fabricated with a two-step method and used as an eco-friendly electrochemical COD sensor. The interlayer TiO 2 was employed to strengthen the adhesion of PbO 2 on the FTO substrates by providing a large TiO 2 /PbO 2 interface area. The effects of the factors including applied potential, supporting electrolyte concentration and stirring speed on the sensing performance were investigated. Under the optimized conditions, linear responses to the COD of water with different COD sources were achieved, and a linear range from 5 to 120 mg/L was obtained in the case of sucrose as the COD source. The relative standard deviations (RSD) were determined to be less than 9% for the glucose solutions with the COD of 7.5, 12.5 and 17.5 mg/L. For real sample analysis, the obtained results were comparable with those measured with the conventional dichromate method, with a relative error less than 11%.
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TALANTA
·
2023
·
IF 6.1
Pengcheng Wu,Jiaren Song,Wanchao Zuo,Jiaying Zhu,Xiangming Meng,Jun Yang,Xinmei Liu,Hui Jiang,Donghui Zhang,Jianjun Dai,Yanmin Ju
Protein glycosylation and other post-translational modifications are involved in many biological processes including growth, development and immune responses, and glycoproteins are also known as biomarkers for cancer, diabetes and cardiovascular diseases. In traditional lateral flow immunoassay (LFIA) for glycoprotein detection, capture antibody (CA) is often required to label targets. However, the production of CA is complicated and expensive, restricting the wide application of LFIA. In this study, we developed a universal boronate affinity CA-independent LFIA method for glycoprotein detection. 4-Mercaptophenylboronic acid (4-MPBA)-modified Au nanoparticles (namely 4-MPBA-AuNPs) were used as LFIA labels, which could generate colorimetric signal and showed outstanding capability to bind glycoprotein. Compared with CA, 4-MPBA molecular as a glycoprotein recognition element had more prominent advantages, e.g., low cost, easy availability and good quality controllability. Take carcinoembryonic antigen (CEA) as model glycoprotein, the limit of detection of this CA-independent LFIA was 1.25 ng/mL by naked eyes, which was 8-fold lower than conventional CA-dependent sandwich LFIA. Significantly, the developed 4-MPBA-AuNPs-based CA-independent LFIA successfully detected 23 CEA-positive samples from 64 suspected human serum samples within 50 min in a nonlaboratory environment, with a 100% accuracy compared to clinical detection method. Therefore, this diagnostic platform could provide an effective tool for point-of-care glycoprotein detection with excellent reproducibility and high specificity.
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Materials Today Communications
·
2023
·
IF 3.8
Lili Jin,Miao He,Ying Chen,Changsheng Liu,Hongyan He
With the burst of pandemic disease in recent years, the demand of microneedle patches (MNPs) has increased rapidly for patient-friendly therapeutic treatment to alleviate the patient suffering. Traditional fabrication processes are complicated and time-consuming, greatly limiting their biomedical applications. To obtain MNPs with designable types for clinic needs, a reverse milling-sputtering technology (RMST) was developed in this work to fabricate scalable masters with gold sputtering for better demoldability. The RMST simplicity allowed for the encapsulation of the clinical analgesic lidocaine into the cryo-MNP (C-MNP), which delivered the drug through the skin to achieve the analgesic efficiency of 96.3%, implying the great potential for clinic analgesia. In addition, RMST also enables the fabrication of MNPs with well-defined shape (50-μm tip diameter), a large area of 35 × 35 mm 2 and various polymer materials. RMST can be a scalable and cost-effective strategy to develop MNPs with individual design, holding great potential for transdermal therapy.
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INORGANIC CHEMISTRY COMMUNICATIONS
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2023
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IF 3.8
Feiyan Wang,Linhong Liao,Xiaohui Liu,Jie Zhang,Fengshou Wu
Glucose is regarded as a clinical biomarker, and the abnormal level of glucose in blood would cause endocrine metabolic diseases. Thus, it is of great significance to develop a simple, accurate, and sensitive method for glucose detection. In this work, we synthesized an iron porphyrin-based porous organic framework (Fe-POF) through a facile alkylation reaction between 5,10,15,20-tetra(4-aminophenyl) porphyrin (TAPP) and cyanuric chloride. Fe-POF exhibited excellent peroxidase-like activity and could be used to detect hydrogen peroxide and the molecules associated with hydrogen peroxide such as glucose. The structure of Fe-POF was confirmed through a series of methods, such as FT-IR, SEM, EDS, TEM, and XPS. Fe-POF exhibited excellent peroxidase-like activity, which could catalyze the oxidization of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into blue product (ox-TMB) in the presence of hydrogen peroxide (H 2 O 2 ). Based on this phenomenon, a simple colorimetric assay was constructed for the detection of H 2 O 2 and glucose with high selectivity and sensitivity. The detection limit of H 2 O 2 and glucose was calculated to be 1.8 and 1.39 μM, respectively. Finally, Fe-POF was applied as a colorimetric sensor for rapid detection of glucose in diluted serum samples.
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TALANTA
·
2023
·
IF 6.1
Zhi-Yong Guo,Chen Zhang,Lin-min Chen,Mei-huang Zeng,Qiu-hong Yao,Ting-Xiu Ye,He-zhou Luo,Xiao-mei Chen,Xi Chen
Shell-isolated colloid plasmonic nanomaterials-based nanoreactor is a well-established platform widely applied in catalyst or Surface Enhanced Raman Scattering (SERS) sensors. The potentials versatility of nanoreactor platform is mainly implemented by the well-defined and tailorable structure of colloid plasmonic nanomaterials . Currently, a competitive conjugative-mediated nanoreactor is introduced to determine glucose with SERS. Glucose-conjugating nanoreactor, as convertors of the sensors, are constructed by coordinated deposition colloidal gold nanoparticles with sodium nitroprusside framework (Au@SNF) and covalently bonded 4-mercaptopyridine (4-Mpy) with self-assembly strategy. The nanoreactor contained the signal-amplifier Au@SNF NPs, conjugative-mediated signal receiver 4-Mpy, and signal internal standard molecular CN − . In addition to well-defined morphology and functionality, conjugative-mediated and internal standards method are also employed to benefit the nanoreactor. The two-parameter strategy significantly improves the signal indication and correction. Using this proposed platform, the competitive-mediated nanoreactor provides a quantitative SERS detection of glucose, and extends the applicability of SERS in more complicated and reproducibility analysis. Meanwhile, the nanoreactor based sensors also exhibited better properties to detect glucose in various food samples and bio-samples which provided strongly appliance for glucose sensors.
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EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2023
·Vol. 249
·期 12
·3097-3103
·
IF 3.3
Li Yangyang,Zhu Zhengwei,Qu Wenli,Yang Qing,Liu Yan,Wang Qiao,Duan Shuo,Wu Jine,Gong Zhiyong,Xu Lin
Lead ions are a toxic metal that can enter the human body through the diet and negatively impact human health. A wide variety of foods, such as vegetables, meat, aquatic product, and drinking water, can be contaminated with lead ions. In this study, a highly sensitive immunochromatographic assay (ICA) based on antibody-oriented probe and silver enhancement was established for the qualitative and quantitative determination of lead ions in drinking water. The recombinant protein A (PrA) was used in probe preparation to improve antibody bioactivity while silver enhancement method was applied to enhance the signal reporter of ICA. It was determined that the visual limit of detection and the scanning limit of quantification of the ICA based on antibody-oriented probe and silver enhancement for lead ions in drinking water were 0.2 ng/mL and 0.08 ng/mL, respectively. The results obtained from developed ICA strip and ICP-MS had no obvious difference using statistical analysis of t test. Compared to previously reported ICA methods for lead ions, the developed ICA method enabled analyzing lead ions in drinking water with a superior sensitivity and detection range.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Min Fan,Yan Li,Jingbo Chen,Yuxiang Lin,Shuxia Lai,Shirun Peng,Duo Lin,Jing Wang,Yudong Lu,Shangyuan Feng
Surface-enhanced Raman spectroscopy (SERS) technology has been increasingly integrated with lateral flow immunoassay (SERS-LFA) to enable sensitive and quantitative detection, but SERS-LFA still faces notable challenges. One of the biggest problems is signal variations caused by random distributions of plasmonic SERS immunoprobes. Herein, we develop a sensitive, accurate quantification platform that overcomes this problem with a plasmonic internal standard (PIS), referred to as PIS-LFA. The PIS is achieved by decorating nitrocellulose membranes with Raman reporter molecule-embedded silver-gold alloy nanoparticles . By simultaneously measuring signals of plasmonic immunoprobes and IS and calculating their intensity ratios for signal normalization, we can mitigate signal variations and improve assay sensitivity. Comparing with SERS-LFA in carcinoembryonic antigen (CEA) detection, we show that PIS-LFA achieves a 1.8-fold higher uniformity and 3.2-fold lower limit of detection. We further demonstrate that PIS-LFA exhibits accurate quantification capability in duplex detection of CEA and neuron-specific enolase (NSE) in human sera by performing spike-recovery experiments and validating with electrochemiluminescence immunoassays. As a proof-of-concept, PIS-LFA differentiates levels of serum CEA and NSE between 12 early-stage lung cancer patients and 12 healthy individuals. We believe that PIS-LFA will open up avenues for affordable, rapid, and non-invasive disease screening in point-of-care settings.
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Materials Today Communications
·
2023
·
IF 3.8
Xiaotong Huang,Mingxia Jiang,Hui Zeng,Junxian Wu,Jiaxin Wu,Xiaojing Liu,Lihua Zhou,Yong Yuan
The high-value conversion of waste resources to carbon materials offers an opportunity to alleviate the resource crisis. Owing to their low cost and easy preservation, iron-based nanoenzymes are receiving increasing attention in the fields of catalysis, detection and biomedicine. In this work, for the first time, paper mill sludge (PMS), which contains iron ions from a flocculant used in paper mill wastewater treatment, is used as a precursor to produce iron-doped carbon quantum dots (Fe-CQDs) by oil bath pyrolysis process. The synthesized Fe-CQDs with carboxyl groups and abundant carbon-deficient structure, exhibit higher peroxidase-like activity. Compared to horseradish peroxidase (HRP), Fe-CQDs have a better affinity for the substrate and better stability. Based on the good affinity of Fe-CQDs for H 2 O 2 , a rapid colourimetric assay for ascorbic acid (AA) detection was developed with a wide range of 1–120 μM and a detection limit of 0.435 μM (3σ/k). The peroxidase-like activity of Fe-CQDs has been proved to originate from their ability to catalyze H 2 O 2 to produce·OH, and successfully applied to determine AA content in the roots and leaves of sweet potato. This work indicates the reliability of the method in practical application and provides a greening strategy for the value-added utilization of solid sludge.
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JOURNAL OF MEMBRANE SCIENCE
·
2023
·
IF 9.5
Faizal Soyekwo,Changkun Liu,Hui Wen,Saleem Raza
Polymeric membranes are the most practical separation technology for extracting target ionic species necessary for water purification and clean energy, but are limited by the multi-scale heterogeneity, and transient and dynamic free volume pores . Modulated interfacial polymerization techniques are emergent concepts for preparing polyamide composite membranes with reduced thickness and homogeneous pore structures to satisfy the requirements for efficient ion separation applications. Herein, N–Cyclic cation-assisted kinetically regulated interfacial polymerization strategy to generate polyamide nanofilm composite membranes with tuned pore network and asymmetric structures for water purification is reported. Experimental characterization techniques and molecular dynamic simulations revealed that the N-Cyclic cations control the kinetic diffusion behaviour of the amine monomers to the aqueous/organic interface conducing to the formation of nanofilm membranes with fine-tuned polyamide network structures. Meanwhile, the N–Cyclic cation–regulated IP process not only affords to decrease the thickness of the formed polyamide nanofilm selective layers but also effectively narrows the effective pore size of the membranes. These membranes with thinner, homogeneous and asymmetric polyamide network structures exhibit enhanced water permeance of 55.7–60.42 L m −2 h −1 bar −1 , and showed Na 2 SO 4 rejection of 99.59–99.66% and MgCl 2 rejection of 98.94–98.96% in nanofiltration separations, thereby outperforming the permeance–selectivity performance of the state-of-the-art polyamide-based membranes. The membranes also exhibit improved forward osmosis performances. We envision that this research will provide insightful foundations for the molecular tuning and construction of the next-generation structurally modulated polyamide nanofilms for various applications in molecular separation and water purification.
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