JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
·
2019
·Vol. 29
·期 4
·538-547
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IF 1.975
Sun L,Ye X,Ding D,Kai L.
The aim of the present study was to evaluate the effects of two well-known natural antioxidants vitamin C (VC) and vitamin E (VE) on the antifungal activity of honokiol against Candida albicans . The broth microdilution method was employed to test the antifungal activities of honokiol with or without antioxidants in the medium against C . albicans strain. Intracellular reactive oxygen species (ROS) and lipid peroxidation were determined by fluorescence staining assay. Mitochondrial dysfunction was assessed by detecting the mitochondrial DNA and the mitochondrial membrane potential. We observed that VC could significantly potentiate the antifungal activities of honokiol while VE reduced the effectiveness of honokiol against C . albicans . In addition, VC accelerated honokiol-induced mitochondrial dysfunction and inhibited glycolysis leading to a decrease in cellular ATP. However, VE could protect against mitochondrial membrane lipid peroxidation and rescue mitochondrial function after honokiol treatment. Our research provides new insight into the understanding of the action mechanism of honokiol and VC combination against C . albicans .
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CHEMICAL ENGINEERING JOURNAL
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2024
·
IF 13.3
Jiaren Du,Tengyue Wang,Yukun Li,Puxian Xiong,Yao Xiao,Ang Feng,Xiaomeng Wang,Kai Jiang,Hengwei Lin
Mechanoluminescence (ML) offers a novel approach to photon emissions without requiring extra electrical input. In recent decade, a variety of ML materials have afforded advantageous prospect for various potential applications. Nevertheless, the material composition optimization and application progress are limited by the time-consuming and complicated preparation methods. Here, a one-step rapid microwave-assisted solid-state (MASS) synthesis approach is firstly employed for the preparation of CaZnOS-based quaternary ML materials with lanthanide/transition metal dopants and variable compositions. Notably, this method only takes a total synthesis duration of 20-minute microwave radiation, which can greatly facilitate the efficient preparation of a variety of ML materials. Moreover, bright host emission from the CaZnOS matrix is unexpectedly induced, providing a new opportunity for manipulating multicolor luminescence. Finally, we have proposed that the people’s writing characteristics can be analyzed by using these ML materials, which offers a great potential for pressure visualization, individualized E-signature anti-counterfeiting applications and beyond. This work not only provides a facile one-step synthesis method for the preparation of CaZnOS-based quaternary ML materials that featuring unique host emission, but also suggests the ML materials to be applied in advanced E-signature and on-demand information identification applications, which is of significance for integrated optoelectronic devices in future.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2026
·
IF 4
Nan Xiang,Xinbo Guo
BACKGROUND Maize ( Zea mays L.), as the most widely cultivated cereal and a dietary cornerstone, provides not only carbohydrates but also essential micronutrients critical for human health, including vitamin C, vitamin E, and carotenoids. However, conventional maize varieties typically exhibit suboptimal levels of these compounds. This study investigated the dynamic accumulation of vitamin C, vitamin E, and carotenoids, along with their transcriptional regulation, during early kernel development (9–15 days after pollination (DAP)) in maize. RESULTS Combined metabolite and transcriptome analyses showed a 23-fold increase in vitamin C content, associated with enhanced expression of the gene VTC2 . Id1 and AGAMOUS-like MADS-box transcription factors (TFs) are putative regulators, targeting the promoters of GalDH and VTC2 to regulate vitamin C biosynthesis. Vitamin E showed isomer-specific fluctuations during early kernel development, mainly associated with the expression of VTE1 , VTE2 , and VTE4 , and potentially regulated by knotted 1 and the AGAMOUS-like MADS-box TFs via VTE2 and VTE3 , respectively. Total carotenoid content tripled, with zeaxanthin becoming dominant by 15 DAP, driven by upregulated PSY1 and CHYB-1 . ERF003 was identified as a candidate regulator of PSY3 . CONCLUSION The increases in vitamin C and carotenoid content, and the fluctuation of vitamin E isomers during early maize kernel development, were associated with the modulation of key genes and putative TFs. These findings reveal theoretical insights and provide candidate genetic targets for future breeding efforts aimed at enhancing micronutrient levels in maize. © 2026 Society of Chemical Industry.
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CHEMICAL ENGINEERING JOURNAL
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2026
·
IF 12.5
Hao Chen,Donghua Yang,Yunhao Hou,Aiguo Feng,Xiyong Chen,Kai Chen,Xinpeng Wang
This report presents the first application of an amide linkage strategy in pyrene-based imine COFs. • Amide groups enhance the polarity and coordination ability of TFPPY-DA-COF channels. • The TFPPY-DA-COF achieved an Au adsorption capacity of 2040 mg g −1 . Pyrene-based covalent organic frameworks (COFs) have received considerable attention for gold recovery, yet challenges remain in terms of adsorption capacity and economic cost. Herein, we initially employed an amide linkage strategy to construct a pyrene-based imine COF for gold recovery. The introduction of amide groups enhanced the polarity and coordination ability of the pore walls, enabling amide-linked COF to exhibit an Au uptake of 2040 mg g −1 , which is approximately twice that of its non-amide counterpart, and superior to most previously reported pyrene-based COFs. Moreover amide-linked COF also demonstrates excellent selectivity (distribution coefficient for Au exceeding 2.74 × 10 5 ; maximum separation factor > 6000) and outstanding performance in practical applications. Notably, the ligands used to synthesize both pyrene-based COFs are low-cost, and the captured Au particles can reach micrometer-scale sizes, which facilitates their separation and recovery. Further performance tests, combined with characterization and DFT calculations, reveal that the coordination of Au ions by the carbonyl groups in the amide linkages plays a dominant role during adsorption, and the reduction effect of the protonated imine bonds also contributes to improving the adsorption performance. Overall, this work provides a new approach for using COF materials to selectively capture and recover gold, offering potential for practical applications. Download: Download high-res image (141KB) Download: Download full-size image
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Nature Communications
·
2026
·Vol. 17
·期 1
·2565
·
IF 18.1
He Mingbin,Wang Weiwei,Zhou Haiwu,Liu Cong,Zhao Chunbei,Li Jian,Han Yuewen,Qin Yali,Chen Mingzhou
Pyroptosis plays a crucial role in immune defense against infections and endogenous threats by eliminating harmful cells and modulating the immune response through inflammation. However, the natural activation of pyroptosis involves intricate signaling pathways, posing significant challenges for its artificial manipulation in research and therapies. Here, we present DAMAGE ( D e a th Ma nipulation Ge ne), an innovative system that integrates gasdermins within the type III-E CRISPR framework, enabling the specific recognition of target RNA (tgRNA) and triggering pyroptosis. This mechanism allows DAMAGE to selectively identify and eliminate virus-infected, cancerous, and senescent cells, all of which exhibit altered RNA transcriptomes. Additionally, DAMAGE exhibits considerable promise as a platform for mRNA-LNP therapy. Our study highlights the potential of this CRISPR-based system in the controllable induction of pyroptosis, offering an innovative therapeutic strategy for treating RNA-heterogeneous diseases.
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ANALYST
·
2026
·
IF 3.6
Xiangyu Feng,Haibing Zhu,Chuanli Ren,Fu-an Wang,Zhanjun Yang,Guiling Li
Vitamin E plays a vital role in maintaining human health as well as in the prevention and treatment of related diseases. However, current methods for detecting vitamin E suffer from complex instrumentation, high cost, and cumbersome operation, making it difficult to meet the demand for rapid detection in serum samples. Herein, we designed an iron-doped cuprous oxide (Fe-Cu2O) nanozyme for the colorimetric detection of vitamin E in human serum. Impressively, the Fe-Cu2O nanozyme exhibits a 7.2-fold enhancement in peroxidase (POD)-like activity compared with the pure Cu2O nanozyme. In the presence of hydrogen peroxide (H2O2), the Fe-Cu2O nanozyme catalyzes the decomposition of H2O2 to generate hydroxyl radicals (˙OH), thereby oxidizing colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into a blue product (ox TMB). As a reducing agent, vitamin E can fade the blue ox TMB, enabling the quantitative determination of vitamin E. Under optimized experimental conditions, the as-prepared Fe-Cu2O nanozyme achieves a linear detection range of 2–80 µM toward vitamin E with a limit of detection (LOD) of 1.89 µM. This approach holds promise as a straightforward, economical, and highly effective strategy for clinical diagnosis and health screening of vitamin E and other bioactive molecules.
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APPLIED AND ENVIRONMENTAL MICROBIOLOGY
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2026
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IF 4.2
Xiaofang Li,Sen Yang,Yuping Liu,Hua Huang,Xinshuai Zhang,Yin Zhong,Lorenz Adrian,Ping’an Peng
Reductive dehalogenases (RDases) are a family of enzymes that employ a cobamide cofactor and two iron-sulfur clusters to selectively cleave carbon-halogen bonds. The identification of yet-uncharacterized RDases is crucial for advancing our understanding of dehalogenation processes in both natural and engineered environments, particularly for bioremediation. In this study, we developed a heterologous expression system in Escherichia coli DH5α to produce a chloroform-reducing RDase (TmrA) from Dehalobacter sp. UNSWDHB, using a pTrcHisA vector without requiring an additional vector for vitamin B12 transporters. By co-expressing the pRSFDuet-BtuCEDFB plasmid, we further enhanced active TmrA production without relying on the T7 promoter system. Using this system, we achieved the first heterologous expression of an RDase (the debrominase Ros_A3X954) from a marine heterotrophic Roseobacter strain, without chemical cofactor reconstitution. Ros_A3X954 catalyzes conversion of 2,6-dibromophenol to 2-bromophenol with a specific activity of 21.8 ± 1.0 nmol s−1 mg−1. The enzyme exhibited optimal activity at pH 6.0 and demonstrated significant thermal stability by maintaining full catalytic capacity across a broad temperature range (20–50°C), a key advantage for applications in thermally fluctuating marine environments. These findings expand our understanding of RDase functional diversity in common marine microbiota and highlight the significant application potential of Roseobacter for bioremediation of bromophenol pollution.
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Nature Communications
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2026
·
IF 18.1
Yidan Chen,Shu Wang,Xilu Ye,Chenghui Lv,Jialin Wei,Yingxin Zhang,Jianfeng Ping,Yibin Ying,Lingyi Lan
The human fingertip skin, with its interlocked epidermal-dermal architecture and dense tactile receptors, enables unique perception and inspires the design of innovative electronic skin (e-skin). However, most e-skin materials fail to replicate the hierarchical integration of composition, structure, and function found in natural skin. Drawing direct inspiration from the dermal extracellular matrix–a composite hydrogel reinforced by a collagen-hyaluronic acid, here we report an e-skin that mimics key composition and architectural features of human skin. By incorporating sodium lactate and montmorillonite nanosheets into a gelatin matrix, we engineer a biomimic gel with mechanical and hydration properties similar to those of natural skin through dynamic ionic crosslinking and hydrogen bond networks. The microstructure resembling the dermal papillae is designed using a sandpaper-templating strategy. The resulting iontronic sensor achieves high sensitivity (466.3 kPa −1 ), rapid response (47 ms), and a wide pressure detection range (20 Pa–2000 kPa). We further develop a stretchable, ultrathin, hand-shaped iontronic sensor array that integrates seamlessly with a dexterous robotic hand, achieving precise, nondestructive grasping and high-fidelity, multichannel pressure mapping. Hydrogels are promising materials for electronic skins and devices, though it is challenging to mimic the composition and architectural features of human skin. Here the authors use a sandpaper templating strategy for a gelatin based hydrogel electronic skin.
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ADVANCED MATERIALS
·
2026
·
IF 29.1
Huan Yang,Hengtian Zhu,Wenyu Teng,Heyu Huang,Zuoxiu Tie,Zixu Li,Zhenning Qi,Songtao Yuan,Zizhong Hu,Fei Xu
Precise intraocular pressure (IOP) management and rapid intervention preserve glaucoma vision, yet current ocular delivery systems lack in situ dosage verification, causing treatment blind spots. Here, we develop a wireless therapeutic smart contact lens (SCL) containing a rapid voltage-triggered drug delivery system with a real-time dosage monitoring sensor. The e-interwoven design integrates drug delivery and sensing electrodes in an ultra-thin (17.4 µm) tri-interdigital configuration, which enables the electric field to interact with the drug-loaded hydrogel in the same spatial region. A frequency-matching strategy boosts voltage coupling to drive a brimonidine tartrate-loaded hydrogel to achieve high concentrations in the aqueous humor in 20 min, significantly faster than topical eye drops. The built-in drug dose sensor has a high accuracy <2.8 µg, benefiting from the great linear correlation between wireless frequency drift and release dosage. In acute glaucoma rabbit models, this SCL suppresses peak IOP elevation by 85.7% compared to eye drops. This SCL presents a highly integrated therapeutic platform demonstrating potential for rapid and precise glaucoma treatment.
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Antibiotics-Basel
·
2026
·Vol. 15
·期 4
·377
·
IF 5.5
Jinlong Li,Liushen Lu,Kai Chen,Ting Qin,Jun Xie,Ping Fang,Bingwen Xi
Background/Objectives: The frequent use of antibiotics has led to increasing drug resistance inAeromonas hydrophila; therefore, there is an urgent need to develop novel antimicrobial agents to prevent and control bacterial diseases in aquaculture. Allicin E (ALE) is derived from garlic (Allium sativumL.), a plant extensively used in traditional medicine for treating infections. This study aimed to evaluate the potential of ALE againstA. hydrophila, a major aquaculture pathogen, by investigating its antibacterial efficacy, mechanisms of action, and in vivo protective effects.Methods: The minimum inhibitory and bactericidal concentrations (MIC/MBC) were determined by broth microdilution. Antibacterial mechanisms were investigated through ROS detection, electron microscopy, fluorescent staining, and content leakage measurement. In vivo efficacy was evaluated inCarassius auratus gibelioby monitoring survival rates and bacterial loads, analyzing immune and antioxidant biomarkers, and histopathological analysis afterA. hydrophilachallenge.Results: ALE exhibited potent antibacterial activity (MIC = MBC = 8 μg/mL), achieving complete bacterial elimination within 1 h and showing a low resistance propensity. Mechanistically, ALE induced ROS accumulation, causing oxidative damage that disrupted membrane integrity and facilitated the leakage of cellular contents. In vivo, ALE significantly enhanced fish survival, reduced bacterial loads, modulated inflammatory cytokines, boosted antioxidant enzyme activities (SOD and CAT), and alleviated tissue damage.Conclusions: ALE possesses potent in vitro antibacterial activity and exerts an inhibitory effect on bacteria-induced inflammatory responses, effectively combatingA. hydrophilathrough a multi-target mechanism and enhancing host resistance.
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ACS Applied Nano Materials
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2026
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IF 5.8
Kun Lu,Liyu Chen,Yaqi Fan,Wenhao Zhang,Qian Liu,Shasha Qu,Xiao Kong,Yanhang Ma
Zeolite Beta, a prototypical intergrowth zeolite with a three-dimensional (3D) system of intersecting 12-member-ring micropore channels, is extensively employed as a versatile solid-acid catalyst. Its structure typically appears as a nanoscale intergrowth of polymorphs arising from the stacking disorder of topologically equivalent layers. Rational regulation of the Beta polymorph composition is, therefore, crucial for optimizing catalytic performance. Here, we report a heterostructure seed-assisted crystallization strategy to synthesize a polymorph E-enriched Beta zeolite (Beta-ER). The 3D electron diffraction, high-resolution transmission electron microscopy imaging, and DIFFaX simulation show that Beta-ER is an intergrown structure of polymorph E/D with polymorph E as the main phase (∼80%). Powder X-ray diffraction, Fourier transform infrared, Raman, and 19F magic-angle spinning nuclear magnetic resonance characterizations suggest that ITQ-1 seeds partially deconstruct in the early stage of crystallization and generate secondary structural building units, which subsequently promote the formation of Beta-ER. In the etherification of FA, Beta-ER with moderate acidity and open pore channels delivers a higher furfuryl ethyl ether (FEE) yield (75.4%) after 30 min at 393 K, outperforming the commercial ZSM-5 (14.2%), Beta (55.9%), and BEC (57.2%) zeolites. Beta-ER maintained stable activity after four regeneration cycles. Density functional theory calculations confirm that substituting framework Al with Ge increases the energy barrier for the ring opening of FEE, thereby suppressing the formation of byproducts. In addition, kinetic analysis and molecular dynamics simulations reveal that the interconnected channel system of Beta-ER facilitates the diffusion of substrates and products.
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ADVANCED FUNCTIONAL MATERIALS
·
2026
·
IF 19.9
Ziyi Gong,Ke Zheng,Qian Wang,Ruoyu Lai,Jian Zou,Xin Guo,Xiao Chen,Wei Wu
The growing demand for integrated temperature-pressure sensing in human-machine interaction and health monitoring makes smart textiles an ideal medium due to their comfort, breathability, and flexibility. However, a key challenge is finding a reliable method to integrate high-performance dual-mode sensors into textile substrates while ensuring effective signal decoupling. Herein, a flexible, ultrathin, and signal-decoupled temperature-pressure dual-mode sensing electronic textile is proposed. The resistive temperature sensing unit utilizes a multi-stage phase transition mechanism to achieve an ultrahigh temperature coefficient of resistance (up to 125.9%/°C) and a broad temperature sensing range (0°C–50°C). The capacitive pressure sensing unit exhibits high sensitivity (up to 62.3 kPa −1 ) and rapid pressure response and recovery times (30 ms/60 ms). By integrating machine learning algorithms, precise recognition of different objects being grasped is achieved. Furthermore, through the fabrication of a sensor array, spatial mapping of pressure and temperature distributions on a simulated body surface is successfully realized, offering a promising route toward developing versatile health-monitoring platforms.
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